This invention is directed to methods of reducing incidence or reducing severity or preventing progression of or preventing or treating or preventing
pathogenesis of a
cancer in a subject, wherein said subject is predisposed to, showing early indications of or suffering from
cancer, or methods of reducing
intracellular bacterial infection in subjects at risk for or suffering from
carcinoma, or from pre-neoplastic or metaplastic tissue changes, comprising contacting at least one
cell in an affected tissue in said subject with an agent reducing
intracellular bacterial infection in affected tissue, reducing localization of
intracellular bacteria proximally to nuclei in cells of affected tissue, or a combination thereof. This invention is further directed to methods of personalized optimized diagnosis and therapy in a subject, comprising screening a subject predisposed to, or at risk for
cancer and identifying whether the pre-cancerous or cancerous tissue in the subject is intracellularly infected with
bacteria, optionally assessing the intracellular localization of the
bacteria-containing compartment to determine a distance of same from a
nucleus in the
cell; optionally assessing the
oncogene profile expression in the subject, and further scoring the subject in terms of the prognosis and potential for therapy and then treating the subject to reduce the intracellular bacterial burden, promote re-localization of the bacterial compartment to be at a further distance from the
nucleus, successfully eradicate intracellular bacterial infection, prevent or reduce
cell-to-cell spread of the bacteria or any combination thereof. Compositions and kits for effecting the methods of this invention are also provided. This invention still further provides a
system for identifying patients who would benefit from adjunctive
antimicrobial therapies, enhancing treatment outcomes by providing comprehensive microbial feature characterization, including
spatial localization of key microbes associated with
treatment resistance in an integrated platform with advanced
machine learning algorithms, ultimately providing a personalized
patient assessment, enhancing treatment outcomes and optimizing therapeutic strategies.