268 results about "Colloidal gold" patented technology

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting feline immunodeficiency virus p24 protein and application thereof. The monoclonal antibody pair is monoclonal antibody 1D8 and monoclonal antibody 1H10, which can specifically recognize feline immunodeficiency virus p24 protein, and the heavy chain and light chain variable region complementarity determining region sequences of 1D8 and 1H10 are clear, and are respectively shown as SEQ ID NO. 1-12. The antibody pair can effectively avoid cross reaction, and significantly improve the accuracy and sensitivity of detection; the colloidal gold detection test strip constructed based on the antibody pair is simple and fast in operation, is suitable for early antigen detection of FIV infection, solves the problem that existing antibody detection cannot distinguish natural infection and vaccine immunization, and provides a reliable technical means for on-site screening and disease prevention and control of FIV.

The invention relates to the technical field of biotoxin detection, and discloses a multidimensional data analysis-based fungaltoxin six-in-one detection card intelligent interpretation system and method.The method comprises the following steps: collecting first toxin detection data of six fungaltoxins, including a fluorescence response time sequence, a colloidal gold response time sequence and an environment variable time sequence; performing feature extraction on the second toxin detection data to obtain second toxin detection data; then establishing a historical detection database, constructing and training a mycotoxin classification model, and obtaining a preliminary recognition result of the six mycotoxins according to the second toxin detection data and the classification model; and finally, carrying out weighted fusion on the preliminary recognition result in combination with an environment variable time sequence to obtain an accurate recognition result. According to the invention, intelligent interpretation of mycotoxin detection is realized, and the detection accuracy and efficiency are greatly improved.

The invention relates to the technical field of colloidal gold detection, and discloses a colloidal gold multi-index synchronous detection system for AI multi-task scheduling. The system comprises a spectral feature decoupling module which separates overlapped spectral responses based on a graph convolution network and generates a spectral fingerprint spectrum; the multi-index quantization module analyzes the nonlinear mapping relation through a variational auto-encoder to generate a quantization decision vector; the fluid dynamic modeling module is combined with a Navier-Stokes equation to invert a sample diffusion path; the signal drift suppression module suppresses background interference by applying a generative adversarial network; the task scheduling engine module adopts a Monte Carlo tree search strategy to allocate computing resources; the cross interference compensation module generates a compensation coefficient matrix by using a tensor decomposition algorithm; and the feedback module controls the micro-valve array to optimize the detection synchronism. All the modules cooperate to achieve multi-index synchronous detection, the detection precision, efficiency and anti-interference capability are improved, and the system is suitable for the fields of medical diagnosis, food safety detection and the like.

The invention belongs to the technical field of biology, and relates to a latex immunochromatography test strip for rapidly detecting porcine epidemic diarrhea virus (PEDV) and a preparation method of the latex immunochromatography test strip. The test strip comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, a quality control line C and a detection line T are arranged on the nitrocellulose membrane. A latex microsphere labeled PEDV N protein monoclonal capture antibody is sprayed on the combination pad, a detection line T is coated with a PEDV N protein monoclonal detection antibody 1E1, and a quality control line C is coated with goat anti-mouse IgG. The test strip can specifically recognize PEDV, and does not react with other porcine intestinal coronaviruses with similar clinical symptoms. The test strip is good in specificity, relatively high in sensitivity and simple to operate, a result is interpreted within 10 minutes, and compared with a traditional colloidal gold detection technology, the stability and the color development effect are obviously improved.

The embodiment of the invention provides a preparation method of a photoelectric biosensor and the photoelectric biosensor, and relates to the technical field of photoelectric sensing, and the preparation method comprises the following steps: preparing a biomass derived hierarchical porous carbon PBAC material, preparing PBAC interface blending adsorption layer carbon slurry according to the biomass derived hierarchical porous carbon PBAC material, and preparing working electrode slurry; performing chemical etching on ITO conductive glass to prepare an electrode substrate, printing the working electrode slurry on the electrode substrate by using a screen printing technology, and printing PBAC interface blending adsorption layer carbon slurry on the electrode substrate to obtain a working electrode; a colloidal gold solution is dropwise added to the working electrode, after drying, a probe solution is dropwise added to the surface of the working electrode again, incubation is conducted in a constant-temperature box, and the photoelectrochemical biosensor is obtained after the reaction is completed. According to the invention, the biocompatibility of the photoelectric biosensor is enhanced, and the stability and sensitivity of the sensor are improved.

The invention discloses a trimethoprim hapten as well as synthesis and application thereof. The structure of the trimethoprim hapten is as shown in a formula I. The trimethoprim hapten provided by the invention can fully expose the antigenic determinant of trimethoprim and maintain the spatial conformation of trimethoprim, has good immunogenicity, and is mild in synthesis conditions, few in reaction steps, high in yield and capable of reducing the cost of raw materials. The trimethoprim colloidal gold detection product provided by the invention has the characteristics of strong specificity and high sensitivity, can realize accurate and rapid detection of trimethoprim in a sample, and has no cross reaction with other drugs. And the formula I of the # imgabs0 # is shown in the specification.

The invention relates to a line elimination method immunochromatography test paper, which is formed by bonding a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad on a bottom plate in a mutual overlapping manner in sequence, and the nitrocellulose membrane is provided with a C line for contrast and a T line for detection, a colloidal gold compound marked by streptavidin and a colloidal gold compound marked by rabbit IgG (immunoglobulin G) are sprayed on the combination pad; a T line on the nitrocellulose membrane is coated with an FITC labeled antibody, and a C line on the nitrocellulose membrane is coated with anti-IgG. The line elimination method immunochromatography test paper can eliminate lines when the cutting proportion of the probe reaches 50%, so that the detection sensitivity is greatly improved, and the line elimination method immunochromatography test paper can be applied to target gene detection products in a CRISPR (clustered regularly interspaced short palindromic repeats) mode, including genotyping of pathogenic microorganisms, SNP (Single Nucleotide Polymorphism), insertion and deletion, structural variation, methylation modification site detection or drug resistance gene identification.

The invention provides an anti-influenza A virus nano antibody and application thereof, the nano antibody comprises a heavy chain variable region, the heavy chain variable region comprises CDR1, CDR2 and CDR3, and the CDR3 of the heavy chain variable region is selected from an amino acid sequence as shown in SEQ ID NO: 8 or SEQ ID NO: 16. The anti-influenza A virus nano antibody can be combined with various influenza A viruses, including subtype influenza viruses such as H1N1, H2N2, H3N2 and H7N9, has strong broad spectrum, has different binding epitopes of the two nano antibodies, and can be applied to double antibody sandwich method detection, enzyme-linked immunosorbent assay and colloidal gold detection. According to the invention, multivalent transformation is also carried out on the nano antibody to obtain a dodecavalent hexamer cyclic structure nano antibody, so that the application scene of the anti-influenza A virus nano antibody can be further extended.

The invention provides a naked eye visual detection method based on CRISPR / Cas reaction system nucleic acid detection and a kit thereof, in the method, a solution of a CRISPR / Cas reaction system is prepared, the solution contains a Cas enzyme with trans-cleavage ability, crRNA and an SSA capture probe, the SSA capture probe is constructed as a recognition element by combining a CRISPR / Cas signal amplification system, and the SSA capture probe is used for detecting nucleic acid in the CRISPR / Cas reaction system. An artificially programmed CG probe with a color developing function, which specifically responds to an SSA probe, is used as a signal transduction element, the SSA probe is used for effectively capturing the CG probe, target nucleic acid is used for activating the activity of CRISPR / Cas trans-cleavage, and the SSA and the CG probe are cleaved, so that colloidal gold is in a free state in a solution, and after magnetic separation is observed by naked eyes, the CG probe and the SSA probe are separated by magnetic force. When the detected sample is positive, the supernatant color is pink, the supernatant of the negative sample and the supernatant of the blank sample are in a colorless state, and the target nucleic acid is detected by distinguishing the colors.

The invention discloses a double-recognition colloidal gold detection method for o-dihydroxy octadecenoic acid, which is characterized in that phenylboronic acid probe molecules are introduced on a colloidal gold probe, and a detection line is coated with a quaternary ammonium salt compound, so that double capture and signal amplification of o-dihydroxy octadecenoic acid metabolites in tears are realized; therefore, the o-dihydroxy octadecenoic acid metabolite in the tears can be rapidly and quantitatively analyzed. Complex sample pretreatment steps and expensive equipment requirements are reduced, so that the detection process is more convenient.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting feline coronavirus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 3C6 and 6H9, 3C6 serves as a coating antibody, 6H9 is used for colloidal gold labeling, and efficient and specific sandwich detection of feline coronavirus NP protein can be achieved. According to the invention, the monoclonal antibody combination is correspondingly applied to an immunodetection platform, and a rapid detection test strip or test paper card based on a colloidal gold immunochromatography technology is constructed. The test paper has high sensitivity to FECV-NP and FIBV-NP recombinant proteins, has no cross reaction with other proteins, and is suitable for rapid and on-site detection.

The invention belongs to the technical field of colloidal gold immunochromatography test strips, and particularly relates to an enhanced colloidal gold immunochromatography test strip pretreatment solution and a preparation method thereof.The pretreatment solution comprises a sample pad pretreatment solution and a conjugate pad pretreatment solution, and the sample pad pretreatment solution comprises trehalose, Tween 20, BSA, a Proclin300 preservative and a borate buffer solution; the conjugate pad pretreatment solution comprises trehalose, Tween 20, BSA, PEG20000, a Proclin300 preservative, polyvinyl alcohol, chitosan and a borate buffer solution, and the enhanced colloidal gold immunochromatography test strip pretreatment solution can enhance the binding force between an antibody on the conjugate pad and colloidal gold while improving the permeability and diffusivity of a sample pad, and maximally maintain the activity of the antibody; and the detection sensitivity and accuracy are always kept at excellent levels, so that the overall performance of a colloidal gold immunochromatography detection product is improved.

The invention discloses detection test paper and a detection kit for detecting telomerase activity and application, the detection test paper comprises a nitrocellulose membrane, the surface of the nitrocellulose membrane is provided with a test area, and a first detection line and a second detection line are printed in the test area; wherein the first detection line and the second detection line are respectively formed by printing a first capture probe sequence and a second capture probe sequence of streptavidin-biotinylation, the first capture probe is used for capturing a DNA neck ring structure extending after telomerase reaction, and the second capture probe is used for capturing a DNA sequence with a fluorophore replaced after telomerase reaction. Meanwhile, the invention discloses a detection kit, which contains a colloidal gold nucleic acid probe solution, can realize rapid dual visual detection of telomerase activity through colloidal gold color development and fluorescence detection, and has the advantages of sensitive and rapid response to telomerase extension products and high sensitivity. The problem of false positive rate in telomerase activity detection can be greatly reduced.

The invention discloses an asymmetric sulfone polymer nanoscale filter membrane as well as a preparation method and application thereof, and belongs to the technical field of membrane materials. The filter membrane comprises a filter membrane main body, the filter membrane main body comprises a pre-filtering layer and a separation layer for intercepting impurities, the average thickness of the separation layer is 0.5-12 microns, and the porosity is 40-80%; the ratio of the maximum aperture of the PMI to the minimum aperture of the PMI of the filter membrane is 1.2-3.5, the retention rate of the filter membrane to 2nm colloidal gold is greater than or equal to 90%, and the ratio of the standard deviation of the area value of the brightness displacement spectrum to the average value of the area value is 0.01-2 when the filter membrane with the retention rate of 2nm colloidal gold is subjected to section brightness measurement; the separation layer structure which is thin and uniform in thickness and the proper pore size and pore size distribution of the filter membrane are combined with high porosity, so that the filter membrane can be ensured to have high flux and high cleanliness while having high interception efficiency, and the filter membrane is suitable for nanoscale purification of a liquid treatment agent used in a semiconductor wet process.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody 3C7 for detecting BK virus VP1 protein and its applications. The amino acid sequences of the heavy chain variable regions CDR1, CDR2, and CDR3 of the monoclonal antibody are SEQ ID NO. 1–3, and the amino acid sequences of the light chain variable regions CDR1, CDR2, and CDR3 are SEQ ID NO. 4–6, respectively. This antibody exhibits high specificity and can recognize the BK virus VP1 protein. The test strip includes a backing plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane, and absorbent paper. The detection method provided by this invention is simple to operate, highly sensitive, and requires no instruments, enabling rapid and visual detection of BK virus VP1 in urine or recombinant protein samples. It is suitable for on-site screening and point-of-care testing and can supplement existing detection methods such as nucleic acid testing.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting canine parainfluenza virus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 5H10 and 1H7, the 5H10 serves as a coating antibody, the 1H7 is used for colloidal gold labeling, and efficient and specific sandwich detection of CPIV / NP antigens can be achieved. The heavy chain and light chain variable region CDR sequences of the two antibodies are clear (SEQ ID NO. 1-12). The invention further provides a rapid detection test strip based on the antibody combination, the rapid detection test strip can be used for on-site screening, early diagnosis and epidemiological monitoring of the canine parainfluenza virus, the problem that an existing detection technology is lack of a high-specificity recognition tool is solved, and the rapid detection test strip has a good application prospect.

The invention discloses a trichina detection kit based on a CRISPR / Cas12a system and click chemistry as well as a use method and application thereof, and belongs to the technical field of immunological detection. The invention provides a trichina detection kit in order to solve the technical problems that a trichina detection method in the prior art is time-consuming and labor-consuming, low in sensitivity and easy to cause leak detection, a CRISPR / Cas12a system signal output probe and a manual click enzyme are complex in preparation process, and a nano material is not easy to store and transport. The kit comprises a CRISPR / Cas12a system, a click reaction system, a 96-well reaction plate, a magnetic bead probe, a colloidal gold probe, a scrubbing solution, a CuSO4 solution, an SA solution and a standard substance. The trichina detection kit provided by the invention has the advantages of high specificity, long-term stability, strong practicability, controllable quality, simplicity in operation, high sensitivity, easiness in storage and the like, can be applied to trichina detection, and has a good market prospect.

The invention discloses preparation and application of a gold electrode and an electrochemical detection device. The preparation method of the modified gold electrode comprises the following steps: activating a polished and ground bare gold electrode with nitric acid, and immersing the polished and ground bare gold electrode into a deoxidized cysteamine (Cyst) solution for 2 hours; washing with double distilled water and soaking for about 12 hours, and removing physically adsorbed Cyst to obtain a Cyst modified gold electrode; immersing the Cyst modified gold electrode into a nanogold solution for 18 hours, taking out and airing to obtain a colloidal gold-Cyst modified gold electrode; and finally, immersing the colloidal gold-Cyst modified gold electrode into Cyst for 2 hours, taking out and airing to obtain the target modified gold electrode. The modified gold electrode prepared by the method, a platinum wire electrode and a silver-silver chloride electrode form a three-electrode system. The modified gold electrode in the three-electrode system has a good electrochemical catalytic oxidation function on the uric acid, the peak current and the content of the uric acid are in a linear relation, and the system can be directly used for detecting the uric acid in the urine.

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The embodiment of the utility model provides a nucleic acid extraction and colloidal gold detection all-in-one machine which is beneficial to reducing artificial participation and improving the working efficiency of nucleic acid extraction and detection. The nucleic acid extraction and colloidal gold detection all-in-one machine comprises a bottom plate; the reagent assembly and the sampling tube assembly are both arranged on the bottom plate, the sampling tube assembly is used for containing a sample, and the reagent assembly comprises a kit, a deep hole plate and a colloidal gold card box; the nucleic acid extraction assembly is movably connected to the bottom plate in the first direction and can stretch into the deep hole plate or be separated from the deep hole plate in the second direction, the second direction is the thickness direction of the bottom plate, and the first direction is perpendicular to the second direction; the liquid transferring assembly is configured to transfer liquid among the sampling tube assembly, the kit, the deep hole plate and the colloidal gold card box.

The disclosure provides a kind of recognition positioning method and system of colloidal gold particles in cryo-EM image, the scheme includes: obtaining the electron microscope image to be identified, and carries out corresponding pretreatment;Wavelet transform is handled to the image after pretreatment based on Atrous algorithm, obtains corresponding wavelet image;For the wavelet image obtained, in turn, binaryzation processing, noise point removal and clustering operation are carried out, obtain the corresponding several clusters of colloidal gold particles in binary image;From the several clusters, the cluster satisfying the preset constraint is screened out, and the average template is obtained based on the obtained cluster satisfying the preset constraint;Template matching is carried out using the average template and the several clusters respectively, and the cluster whose matching result is lower than the preset threshold is filtered out, and the remaining cluster is used as the candidate colloidal gold particle target;The position and size estimation of the candidate colloidal gold particle target obtained are obtained by corresponding the original image.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

A Trichinella detection kit based on the CRISPR / Cas12a system and click chemistry, as well as its usage method and application, belong to the technical field of immunological detection. To solve the technical problems in the prior art that the Trichinella detection methods are time-consuming and laborious, have low sensitivity, are prone to missed detection, and the signal output probes of the CRISPR / Cas12a system, the complex preparation process of artificial click enzymes, and the difficulty in storing and transporting nanomaterials, the present invention provides a Trichinella detection kit, which includes: a CRISPR / Cas12a system, a click reaction system, a 96-well reaction plate, magnetic bead probes, colloidal gold probes, washing solution, CuSO4 solution, SA solution, and standards. The Trichinella detection kit provided by the present invention has the advantages of high specificity, long-term stability, strong practicability, controllable quality, simple operation, high sensitivity, easy preservation, etc., and can be applied to Trichinella detection, with good market prospects.

The utility model relates to a reaction device for preparing colloidal gold, which comprises a sealed reaction cavity communicated with a condensing device. The stirring device comprises a stirring driving device, a stirring rod and a stirrer; the stirring driving device is arranged outside the reaction cavity; the stirring rod is connected with the stirring driving device and extends into the reaction cavity; the stirrer is arranged at the free end of the stirring rod; the center of the stirring rod and the center of the stirrer are hollow bodies which are communicated with each other, and pores are formed in the surface of the stirrer; the reaction kettle further comprises a feeding device, the feeding device is arranged outside the reaction cavity, an outlet of the feeding device is connected with a feeding pipe, and the free end of the feeding pipe extends into the hollow body in the stirring rod, so that materials are added into the stirrer through the stirring rod, and the stirring rod is connected with the stirring rod. And further, the gas enters the reaction cavity through pores in the surface of the stirrer. When the reaction device is used for preparing colloidal gold, the product difference among different batches of production can be reduced.

The invention relates to the technical field of biological detection, in particular to a teletamine detection card as well as a preparation method and application thereof. The invention provides a tiletamine detection card. The tiletamine detection card comprises a bottom plate, a sample loading pad, a colloidal gold pad, an NC (nitrocellulose) membrane and a water absorption pad, a sample loading pad, a colloidal gold pad, an NC (nitrocellulose) membrane and a water absorption pad are sequentially arranged on the bottom plate along the liquid chromatography direction; the colloidal gold pad is coated with a colloidal gold labeled teletamine monoclonal antibody and a colloidal gold labeled first antibody; a detection line and a quality control line are sequentially arranged on the NC membrane along the liquid chromatography direction; the detection line is coated with a teletamine conjugate; the quality control line is coated with a second antibody; the second antibody can be specifically combined with the first antibody. The teletamine detection card and the colloidal gold detection kit provided by the invention have the advantages of good application effect, good specificity and good repeatability.

The invention belongs to the technical field of food safety monitoring and immunoassay detection, and particularly relates to a colloidal gold immunochromatography test strip, a preparation method and application thereof, the colloidal gold immunochromatography test strip comprises a bottom plate, a nitrocellulose membrane, absorbent paper, a conjugate pad and a sample pad; a mycobacterium paratuberculosis monoclonal antibody marked by colloidal gold is adsorbed on the combination pad; a detection line coated with the mycobacterium paratuberculosis monoclonal antibody and a quality control line formed by goat anti-mouse-IgG are arranged on the nitrocellulose membrane; and the bottom plate is a polyethylene chloride bottom plate, so that the MAP in the fresh milk can be rapidly and accurately detected.

The application discloses a method for constructing a multiplex visual nucleic acid detection system, which comprises the following steps: S1, selecting specific conservative gene sequences of multiple target detection objects; S2, designing specific LAMP primers according to the specific conservative gene sequences of the multiple target detection objects selected in S1; S3, marking the specific loop primers designed in S2 and designing specific assimilation probes; S4, placing the primers, the marked primers, the assimilation probes and multiple nucleic acid templates formed in S2 and S3 into the same system for LAMP amplification to form amplification products containing multiple markers; S5, placing the amplification products containing multiple markers in S4 into a binding pad of a detection strip; and S6, under the action of chromatography, the amplification product-marked colloidal gold complex is captured by corresponding antibodies through a detection belt to realize color development. The application realizes double color development by using a visual LFD detection system, namely, UU and MH use different color development systems respectively, so that UU and MH can be rapidly and simply detected.

The invention provides a detection system, in particular, a background fluorescence enhanced quenching immunochromatography method is adopted, laser with smaller light spots is used as an excitation source, and compared with a common background fluorescence quenching immunochromatography method for quantitative detection of the content of a certain antigen, better analysis performance is achieved, and sensitivity is remarkably improved. When the quenching agent colloidal gold is replaced by the nano microspheres with the Raman spectrum signals, the positive and negative properties can be quickly judged by observing the color intensity of the T line with naked eyes, accurate quantification can be realized by measuring the fluorescence signals on the T line, and the detection technology of three signal output modes of measuring the Raman spectrum signals on the T line by using a Raman spectrometer can be used; the detection sensitivity is further improved. In addition, by arranging two detection lines, the effect of joint detection is achieved while high sensitivity is achieved. Therefore, the test piece and the corresponding detection system have very excellent analysis performance.