148 results about "Colloidal gold" patented technology

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting feline immunodeficiency virus p24 protein and application thereof. The monoclonal antibody pair is monoclonal antibody 1D8 and monoclonal antibody 1H10, which can specifically recognize feline immunodeficiency virus p24 protein, and the heavy chain and light chain variable region complementarity determining region sequences of 1D8 and 1H10 are clear, and are respectively shown as SEQ ID NO. 1-12. The antibody pair can effectively avoid cross reaction, and significantly improve the accuracy and sensitivity of detection; the colloidal gold detection test strip constructed based on the antibody pair is simple and fast in operation, is suitable for early antigen detection of FIV infection, solves the problem that existing antibody detection cannot distinguish natural infection and vaccine immunization, and provides a reliable technical means for on-site screening and disease prevention and control of FIV.

The invention relates to the technical field of colloidal gold detection, and discloses a colloidal gold multi-index synchronous detection system for AI multi-task scheduling. The system comprises a spectral feature decoupling module which separates overlapped spectral responses based on a graph convolution network and generates a spectral fingerprint spectrum; the multi-index quantization module analyzes the nonlinear mapping relation through a variational auto-encoder to generate a quantization decision vector; the fluid dynamic modeling module is combined with a Navier-Stokes equation to invert a sample diffusion path; the signal drift suppression module suppresses background interference by applying a generative adversarial network; the task scheduling engine module adopts a Monte Carlo tree search strategy to allocate computing resources; the cross interference compensation module generates a compensation coefficient matrix by using a tensor decomposition algorithm; and the feedback module controls the micro-valve array to optimize the detection synchronism. All the modules cooperate to achieve multi-index synchronous detection, the detection precision, efficiency and anti-interference capability are improved, and the system is suitable for the fields of medical diagnosis, food safety detection and the like.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting feline coronavirus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 3C6 and 6H9, 3C6 serves as a coating antibody, 6H9 is used for colloidal gold labeling, and efficient and specific sandwich detection of feline coronavirus NP protein can be achieved. According to the invention, the monoclonal antibody combination is correspondingly applied to an immunodetection platform, and a rapid detection test strip or test paper card based on a colloidal gold immunochromatography technology is constructed. The test paper has high sensitivity to FECV-NP and FIBV-NP recombinant proteins, has no cross reaction with other proteins, and is suitable for rapid and on-site detection.

The invention discloses an asymmetric sulfone polymer nanoscale filter membrane as well as a preparation method and application thereof, and belongs to the technical field of membrane materials. The filter membrane comprises a filter membrane main body, the filter membrane main body comprises a pre-filtering layer and a separation layer for intercepting impurities, the average thickness of the separation layer is 0.5-12 microns, and the porosity is 40-80%; the ratio of the maximum aperture of the PMI to the minimum aperture of the PMI of the filter membrane is 1.2-3.5, the retention rate of the filter membrane to 2nm colloidal gold is greater than or equal to 90%, and the ratio of the standard deviation of the area value of the brightness displacement spectrum to the average value of the area value is 0.01-2 when the filter membrane with the retention rate of 2nm colloidal gold is subjected to section brightness measurement; the separation layer structure which is thin and uniform in thickness and the proper pore size and pore size distribution of the filter membrane are combined with high porosity, so that the filter membrane can be ensured to have high flux and high cleanliness while having high interception efficiency, and the filter membrane is suitable for nanoscale purification of a liquid treatment agent used in a semiconductor wet process.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody 3C7 for detecting BK virus VP1 protein and its applications. The amino acid sequences of the heavy chain variable regions CDR1, CDR2, and CDR3 of the monoclonal antibody are SEQ ID NO. 1–3, and the amino acid sequences of the light chain variable regions CDR1, CDR2, and CDR3 are SEQ ID NO. 4–6, respectively. This antibody exhibits high specificity and can recognize the BK virus VP1 protein. The test strip includes a backing plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane, and absorbent paper. The detection method provided by this invention is simple to operate, highly sensitive, and requires no instruments, enabling rapid and visual detection of BK virus VP1 in urine or recombinant protein samples. It is suitable for on-site screening and point-of-care testing and can supplement existing detection methods such as nucleic acid testing.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting canine parainfluenza virus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 5H10 and 1H7, the 5H10 serves as a coating antibody, the 1H7 is used for colloidal gold labeling, and efficient and specific sandwich detection of CPIV / NP antigens can be achieved. The heavy chain and light chain variable region CDR sequences of the two antibodies are clear (SEQ ID NO. 1-12). The invention further provides a rapid detection test strip based on the antibody combination, the rapid detection test strip can be used for on-site screening, early diagnosis and epidemiological monitoring of the canine parainfluenza virus, the problem that an existing detection technology is lack of a high-specificity recognition tool is solved, and the rapid detection test strip has a good application prospect.

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The embodiment of the utility model provides a nucleic acid extraction and colloidal gold detection all-in-one machine which is beneficial to reducing artificial participation and improving the working efficiency of nucleic acid extraction and detection. The nucleic acid extraction and colloidal gold detection all-in-one machine comprises a bottom plate; the reagent assembly and the sampling tube assembly are both arranged on the bottom plate, the sampling tube assembly is used for containing a sample, and the reagent assembly comprises a kit, a deep hole plate and a colloidal gold card box; the nucleic acid extraction assembly is movably connected to the bottom plate in the first direction and can stretch into the deep hole plate or be separated from the deep hole plate in the second direction, the second direction is the thickness direction of the bottom plate, and the first direction is perpendicular to the second direction; the liquid transferring assembly is configured to transfer liquid among the sampling tube assembly, the kit, the deep hole plate and the colloidal gold card box.

The disclosure provides a kind of recognition positioning method and system of colloidal gold particles in cryo-EM image, the scheme includes: obtaining the electron microscope image to be identified, and carries out corresponding pretreatment;Wavelet transform is handled to the image after pretreatment based on Atrous algorithm, obtains corresponding wavelet image;For the wavelet image obtained, in turn, binaryzation processing, noise point removal and clustering operation are carried out, obtain the corresponding several clusters of colloidal gold particles in binary image;From the several clusters, the cluster satisfying the preset constraint is screened out, and the average template is obtained based on the obtained cluster satisfying the preset constraint;Template matching is carried out using the average template and the several clusters respectively, and the cluster whose matching result is lower than the preset threshold is filtered out, and the remaining cluster is used as the candidate colloidal gold particle target;The position and size estimation of the candidate colloidal gold particle target obtained are obtained by corresponding the original image.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

The invention relates to the technical field of biological detection, in particular to a teletamine detection card as well as a preparation method and application thereof. The invention provides a tiletamine detection card. The tiletamine detection card comprises a bottom plate, a sample loading pad, a colloidal gold pad, an NC (nitrocellulose) membrane and a water absorption pad, a sample loading pad, a colloidal gold pad, an NC (nitrocellulose) membrane and a water absorption pad are sequentially arranged on the bottom plate along the liquid chromatography direction; the colloidal gold pad is coated with a colloidal gold labeled teletamine monoclonal antibody and a colloidal gold labeled first antibody; a detection line and a quality control line are sequentially arranged on the NC membrane along the liquid chromatography direction; the detection line is coated with a teletamine conjugate; the quality control line is coated with a second antibody; the second antibody can be specifically combined with the first antibody. The teletamine detection card and the colloidal gold detection kit provided by the invention have the advantages of good application effect, good specificity and good repeatability.

The application discloses a method for constructing a multiplex visual nucleic acid detection system, which comprises the following steps: S1, selecting specific conservative gene sequences of multiple target detection objects; S2, designing specific LAMP primers according to the specific conservative gene sequences of the multiple target detection objects selected in S1; S3, marking the specific loop primers designed in S2 and designing specific assimilation probes; S4, placing the primers, the marked primers, the assimilation probes and multiple nucleic acid templates formed in S2 and S3 into the same system for LAMP amplification to form amplification products containing multiple markers; S5, placing the amplification products containing multiple markers in S4 into a binding pad of a detection strip; and S6, under the action of chromatography, the amplification product-marked colloidal gold complex is captured by corresponding antibodies through a detection belt to realize color development. The application realizes double color development by using a visual LFD detection system, namely, UU and MH use different color development systems respectively, so that UU and MH can be rapidly and simply detected.

The present application relates to the technical field of immunochromatography, and in particular, to a test strip for detecting β-amyloid protein and the use thereof. In the present application, colored microspheres are conjugated to a β-amyloid monoclonal antibody via covalent bonds, and then the conjugate is coated onto a conjugate pad. Compared with colloidal gold, the colored microspheres exhibit greater stability and higher sensitivity. Additionally, the colored microspheres feature bright and diverse colors, uniform particle sizes, good monodispersity, and strong reproducibility of detection results. The test strip provided by the present application has the advantages of simplicity, rapidity and high timeliness, requires no additional reagents, instruments and professional personnel, and supports on-site operation. By means of simply applying a to-be-detected sample to a sample loading port of the test strip, a detection result can be interpreted within 15 min. The result interpretation is visual, intuitive, accurate and straightforward, with a low risk of human errors such as false positives and false negatives.

The invention discloses a universal probe for multiple nucleic acid detection, a probe combination and application of the universal probe and the probe combination, aiming at the limitation that the existing nucleic acid detection technology depends on expensive equipment, is complicated to operate and is difficult to realize multiple detection. A bridging detection system is formed by innovatively designing a general probe, a specific probe series A (two ends of which are fixed in sequence and are used for connecting the general probe and a quality control line) and a specific probe series B (one end of which is fixed on a detection line); the test strip structurally comprises a sample pad, a colloidal gold pad (for fixing a universal probe), an NC (nitrocellulose) membrane (containing a plurality of detection lines and quality control lines) and absorbent paper. The method is suitable for single-stranded nucleic acid (such as NASBA amplification products) detection, has high sensitivity, high specificity (no false positive), rapidness (completion within 10 minutes) and multiple detection capability, does not need complex instruments, is low in cost, and is easy to popularize in a basic level.

The application belongs to the field of biological detection, and particularly relates to a specific monoclonal antibody of an AKK bacterial efficacy protein Amuc_1100, a kit, a detection method and application thereof. The antibody pair is 3E10 and 2F7 monoclonal antibodies, the heavy chain and light chain variable region amino acid sequences of 3E10 are SEQ ID NO. 2 and SEQ ID NO. 4, and the heavy chain and light chain variable region amino acid sequences of 2F7 are SEQ ID NO. 6 and SEQ ID NO. 8. The antibody pair has high specificity and high affinity, a colloidal gold rapid detection kit and a double antibody sandwich ELISA quantitative detection kit based on the antibody pair realize rapid screening and accurate quantification of the Amuc_1100 protein, have good linearity, high precision and excellent accuracy, and can be used for detection of the protein in mucoid Akkermansia and quality control and activity evaluation of related probiotic preparations, thereby providing key technical support for related basic research and industrial application.

The present invention belongs to the field of biological detection technology, and specifically relates to a monoclonal antibody combination and application for the detection of Candida albicans enolase protein. The monoclonal antibody combination comprises monoclonal antibodies 5G7 and 5B1. The amino acid sequences of the CDR regions of monoclonal antibodies 5G7 and 5B1 are shown in SEQ ID NO.1-12. The antibody combination has high specificity and binding activity and can be used for the efficient detection of Candida albicans enolase. The colloidal gold detection test strip constructed based on the combination has good sensitivity and specificity, with a minimum detection limit of up to 500pg / ml, and does not cross-react with other common fungi. The present invention provides a reliable tool for the early diagnosis and rapid detection of invasive Candida infections, and has good clinical application prospects.

The application discloses a kind of Gui Shiwei Chinese herbal medicine organophosphorus pesticide rapid detection method and detection kit, by the sample of Gui Shiwei medicine powder to be measured is extracted by acetonitrile solution containing acetic acid, salting-out stratification is taken supernatant;With colloidal gold labeled anti-organophosphorus pesticide small peptide antibody incubation, carry out competition immune response in the microwell plate coated with organophosphorus hapten-protein complex;After washing, the absorbance is determined at 450 to 550nm wavelength, calculate competition inhibition rate and compare with preset first threshold value to determine whether it is over standard;The method is simple and rapid;In addition, for the detection needs of different organophosphorus pesticides, by setting different organophosphorus hapten-protein complex coating area in the microwell plate, the simultaneous detection of multiple organophosphorus pesticides is realized, and the applicability of organophosphorus pesticide residue detection is further improved.

This invention relates to a method for the large-scale production of "I"-shaped test strips for hygiene products, belonging to the field of medical test strip processing. The "I"-shaped test strip is an improvement and breakthrough of conventional test strips, reducing the foreign body sensation and other discomfort caused to users when used in hygiene products without affecting the test strip's detection function. The large-scale production process includes cutting colloidal gold-nitrocellulose boards, attaching colloidal gold-nitrocellulose strips to a substrate, attaching sample pads and absorbent pads to the substrate, laminating a top waterproof membrane, and rolling the formed test strips. This process can continuously produce high-quality "I"-shaped test strip rolls, meeting the diverse production needs of the hygiene product industry.

The invention provides a medetomidine artificial hapten, an artificial antigen as well as a preparation method and application of the medetomidine artificial hapten and the artificial antigen. Specifically, the invention provides a medetomidine artificial hapten of formula (X), the artificial hapten maximally retains the characteristic structure of medetomidine, has an active group capable of being coupled with carrier protein, can be used as an antigenic determinant, and has high sensitivity during immunoassay; furthermore, the invention provides a medetomidine artificial antigen based on the medetomidine artificial hapten, and the medetomidine artificial antigen can be applied to colloidal gold immunochromatography detection of medetomidine and has relatively high sensitivity, detection gradient and relatively good specificity.

The invention relates to a large yellow croaker pyramidal worm rapid detection kit based on an MIRA technology, and belongs to the field of aquaculture pathogen biological diagnosis. The rapid detection kit for the large yellow croaker pyramidal worm comprises a nucleic acid releasing agent, a primer probe group for MIRA amplification and a nucleic acid detection test strip for detecting an MIRA amplification product in a matched manner, the nucleic acid releasing agent is used for releasing nucleic acid in a sample to be detected; the primer probe group comprises an upstream primer, a downstream primer and a probe; the nucleic acid test strip is a colloidal gold lateral flow chromatography test strip. The rapid detection kit for the Pseudosciaena crocea pyramidal can rapidly, efficiently and sensitively detect the Pseudosciaena crocea pyramidal, and provides an effective technical means for differential diagnosis of the Pseudosciaena crocea pyramidal.

The invention discloses a yeast immunochromatography rapid detection test strip and a preparation method thereof, and relates to the technical field of immunodetection.The test strip comprises a functionalized sample pad, a combination pad, a reaction film and a water absorption pad which are in lap joint in sequence; the sample pad is pretreated by a treating fluid containing beta-1, 3-glucanase, casein and TritonX-100, so that in-situ wall breaking and interference resistance can be realized; the reaction film detection line is coated with mannose binding lectin and a saccharomycetes specific monoclonal antibody, the binding pad fixes different epitope detection antibodies labeled by colloidal gold, the preparation method comprises the steps of functionalizing the sample pad, coating the reaction film, preparing the binding pad and assembling the test strip, and the matched kit comprises the test strip and a phosphate buffer diluent with a specific pH value. The method can realize extraction-free one-step rapid detection of saccharomycetes, has both specificity and stability, and is suitable for multi-scene screening.

The invention discloses a fracturing tracing monitoring method based on nano colloidal gold particles, and relates to the technical field of fracturing tracing. The nano colloidal gold particles prepared through special design serve as a tracer agent to be injected into a stratum along with fracturing fluid, a flowback signal is tested, and the fracturing tracing monitoring task which cannot be achieved by a common tracer agent at present is completed. The method comprises the implementation steps of tracer agent injection, flowback fluid sampling, tracer agent signal detection, curve drawing, fracture development degree evaluation and the like, and data support can be provided for fracture volume inversion, fracture complexity and fracture effect evaluation through implementation of the method. The method has the characteristics of rapid detection, accurate quantitative analysis, high detection sensitivity, accurate data, no background interference and the like, and has unique advantages in the field of fracturing tracing monitoring.

This invention relates to the field of protein extraction technology, and in particular to a highly efficient method for extracting and preserving natural BICC1 protein from serum, comprising the following steps: 1) preparation of aminated magnetic Fe3O4 nanoparticles; 2) preparation of Fe3O4@Au nanoparticles; 3) preparation of BICC1 template protein; 4) binding of BICC1 template protein; 5) immobilization of BICC1 template protein; 6) elution of BICC1 template protein to prepare BICC1 magnetic molecularly imprinted polymer microspheres; 7) purification of natural BICC1 protein. This invention features a simple method with mild reaction conditions. The colloidal gold layer of the magnetic molecularly imprinted polymer achieves highly efficient adsorption of template molecules, and the outermost polymer immobilization layer, composed of neutral epoxy groups, reduces non-specific adsorption, enabling efficient and specific extraction of natural BICC1 protein directly from serum.

1. Name of the product in this design: Tester (Pen-shaped Colloidal Gold Tester). 2. Purpose of this design: To be used for the qualitative or quantitative detection and analysis of analytes in human samples using colloidal gold immunochromatography. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 1.

The invention discloses a method for detecting pseudomonas aeruginosa in a beverage, which comprises the following steps: step 1, mixing and incubating a pseudomonas aeruginosa aptamer solution and a colloidal gold solution, then adding a NaCl solution, uniformly mixing and reacting to obtain a mixed solution; step 2, mixing and incubating the mixed solution and a to-be-detected sample solution, then adding a Tb-MOFs solution, and uniformly mixing and reacting to obtain a to-be-detected precursor solution; and 3, measuring a fluorescence signal of the precursor solution to be measured. According to the method, detection can be realized by using a fluorescence spectrometer, the detection range is 1-106 CFU / mL, and the detection limit is 0.63 CFU / mL. The terbium-based metal organic framework material is used as a fluorescence signal element for the first time, and the aptamer is used as a target recognition element, so that the fluorescent probe has good anti-interference capability and is suitable for solution systems such as water and beverages. In addition, the method does not need strain culture and has the advantages of simplicity and convenience in operation, high sensitivity and the like.

The application relates to the technical field of allergy detection, in particular to an inhaled allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit; the detection object is pollen, dust mites and animal dander; the kit comprises colloidal gold labeled mouse anti-human lgE antibodies arranged on a test strip combination pad; and pollen antigens (Art v1, Amb a1, Bet v2), dust mite antigens (Der f1, Der p1) and animal dander antigens (Can f1) arranged on a test strip nitrocellulose membrane. The kit provided by the application can quickly identify the allergen of inhaled allergic symptoms, classify the severity of allergic symptoms, and the identification result has a high accuracy.

The utility model relates to the technical field of detection cards, and discloses a biotoxin detection card which comprises a shell and detection test paper, the shell comprises a bottom shell and a shell cover detachably installed on the bottom shell, the upper side face of the bottom shell is concaved inwards to form a driving groove and a containing groove which are communicated, and the containing groove is used for containing the detection test paper. A limiting piece for limiting the test paper in the placing groove is arranged in the driving groove in a sliding manner; the detection test paper comprises a bottom plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and an absorption pad are sequentially arranged on the bottom plate, a detection line is arranged on the nitrocellulose membrane, a sample adding hole corresponding to the sample pad is formed in the shell cover, and an observation opening corresponding to the nitrocellulose membrane is formed in the shell cover. The shell cover is detachably mounted on the bottom shell, meanwhile, the limiting piece moves in the driving groove, and the detection test paper is limited in the containing groove, so that the detection test paper can be better taken and placed, namely, the detection test paper of the detection card can be replaced, and the shell is repeatedly used.

The invention relates to a respiratory syncytial virus antigen detection kit, and relates to the technical field of biomedical detection, the respiratory syncytial virus antigen detection kit comprises a respiratory syncytial virus antigen detection reagent strip, the reagent strip comprises a sample pad, a colloidal gold conjugate pad and a nitrocellulose membrane which are connected in sequence, the colloidal gold combination pad is coated with a colloidal gold labeled nano antibody which is specifically combined with the respiratory syncytial virus, and the nano antibody comprises a CDR sequence as shown in SEQ ID NO: 2-4. The kit provided by the invention has the effects of high detection sensitivity, good specificity and short detection time.