2228 results about "Colloidal gold" patented technology

The invention provides a detection kit of IgM/IgG antibodies of novel coronavirus (SARS-CoV-2), and relates to the technical field of biology. According to the detection kit of IgM/IgG antibodies of novel coronavirus (SARS-CoV-2), the IgM/IgG antibodies of novel coronavirus (SARS-CoV-2) are detected through adoption of a colloidal gold capture method, a colloidal gold indirect method and a colloidal gold double antigen sandwich method, wherein adopted antigens are highly active recombinant antigens which are obtained through fusion expression of N protein fragments and S protein dominant epitope fragments, and colloidal gold is labeled indirectly, so that steric hindrance is reduced, and the activity, reaction consistency and accuracy of the antigens are increased. Since a sample pad is pretreated, the interference of complex components in a blood sample to a reaction can be reduced greatly, the detection kit has no requirements for instruments during detection, simple and flexible operation, direct and manual interpretation of results and a short test time, the results can be interpreted in only 15 minutes, and the test results are accurate.

The invention provides a human mycoplasma pneumoniae gold-marked silver-stained immunochromatographic assay kit and a preparation method and application thereof. The assay kit comprises a detection card and a silver-stained sensitivity-enhanced pad, wherein the detection card is composed of a bottom plate, a sample pad, an absorbent pad, a conjugate pad and a detection layer; the conjugate pad is coated with a colloidal gold-marked polyclonal antibody mixture of colloidal gold marked rabbit anti-human mycoplasma pneumoniae P1 protein and P30 protein; the detection layer is composed of a solid phase nitrocellulose membrane with a detection line and a quality control line; the detection layer is bonded on the bottom plate, the conjugate pad and the absorbent pad are partially overlapped with the detection layer respectively and are bonded with the detection layer and the bottom plate respectively; the sample pad and the conjugate pad are partially overlapped to be bonded with the conjugate pad and the bottom plate respectively; and the silver-stained sensitivity-enhanced pad consists of a AgNO3 pad and a restoring pad. The human mycoplasma pneumoniae gold-marked silver-stained immunochromatographic assay kit can effectively improve the detection sensitivity of the human mycoplasma pneumoniae, has the strong specificity and has the high application value in the aspects of clinical diagnosis of human mycoplasma pneumoniae, etiology identification, epidemiological investigation and the like.

Compositions and methods for producing monoclonal antibodies and their derivatives reactive against bioactive lipid targets are described. These compositions include derivatized lipids, each of which comprises a bioactive lipid that having a polar head group and at least one hydrocarbon chain (e.g., a lysolipid such as lysophosphatidic acid or sphingosine-1-phosphate) in which a carbon atom has been derivatized with a pendant reactive group; immunogens made by linking a derivatized lipid to a carrier moiety (e.g., a carrier protein, polyethylene glycol, colloidal gold, alginate, or a silicone bead); monoclonal antibodies and derivatives produced by immunizing an animal with such an immunogen; and therapeutic and diagnostic compositions containing such antibodies and antibody derivatives. Methods for making such derivatized lipids, immunogens, and monoclonal antibodies and derivatives, methods for detecting such antibodies once generated, and therapeutic and diagnostic methods for using such antibodies and derivatives, are also described.

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

The invention provides a test paper reading method and a test paper reading device, which can read dry chemical test paper strips, colloidal gold immunochromatograohic assay test paper strips/cards and rapid pesticide residue test cards, are easy to operate, need no extra detection instrument and equipment, can automatically judge and read the detection result by only acquiring corresponding developing pictures through electronic equipment such as computers or mobile phones, have reliable testing accuracy and are particularly suitable for personal and household wide application. The method comprises the following steps: A. acquiring images; B. processing the acquired images; C. performing negative and positive discrimination; and D. performing concentration conversion on a sample which is discriminated to be positive in the step C through a concentration-gray scale relation to obtain a concentration value of the to-be-tested paper card/strip. The device comprises a shell and a built-in standard gray scale strip, wherein the shell is provided with a standard gray scale strip display window and is also provided with a first card slot, a second card slot and a third card slot for arranging the colloidal gold card, the rapid pesticide residue test card and the dry chemical test paper respectively.

The present invention provides binding assays, referred to here as fluorescence proximity assays or FPA. The inventions detect binding of target molecules in a sample to a molecular probe or probes that specifically bind or hybridize to those molecules. In particular, the molecular probes are immobilized to a bead or particle, such as colloidal gold, the reflects fluorescent energy from a fluorophore. The derivatized beads are contacted to a sample of fluorescently labeled target molecules, and binding of the target is indicated by an increase in the fluorescent signal. Kits are also provided that contain materials and reagents to performing a fluorescence proximity assay.

The invention provides a full-scale CRP colloidal gold immunoturbidimetric assay kit. A quantitative assay purpose is reached through enhancing the turbidity by the colloidal gold. The method used by the kit provided by the invention solves a problem that the generation of a precipitate after a latex reinforced immunoturbidimetric reaction goes against biochemical instrument cleaning. The kit provided in the invention comprises a reagent R1 and a reagent R2, wherein the reagent R2 is a proper buffer solution; and the reagent R2 is a buffer solution of the colloidal gold combined with an antihuman CRP antibody. The kit has the characteristics of high sensitivity, strong specificity and good stability, can be used for assaying the content of the CRP in serum or blood plasma, and is suitable for clinical fully-automatic biochemical analyzers. The CRP assay sensitivity of the kit can reach 0.01mg/L, and the upper CRP assay limitation of the kit is 500mg/L.

A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.

The invention discloses a chromatographic detection kit based on an aptamer, as well as a preparation method and detection method of the kit. The test paper of the kit includes a bottom board, and a sample pad, a bonding pad, a cellulose nitrate film and a water absorption pad, which are adhered on the bottom board and overlap one another; a detection line is arranged on the side of the cellulose nitrate film close to the bonding pad, and a quality control line is arranged on the side of the cellulose nitrate film close to the water absorption pad; an A aptamer labeled by colloidal gold is applied on the bonding pad; a composite containing a B aptamer and streptavidin is applied on the detection line; streptavidin is applied on the quality control line; and both of the A aptamer and the B aptamer are aptamers labeled by biotin and capable of specifically identifying a same test object. The chromatographic detection kit can display the result in five minutes only by directly dropping a diluted sample to be tested into a sample hole, is simple, quick, sensitive, easy to read result, and convenient and simple to prepare, and so on, and has the advantages of simplicity in operation and high specificity without using antibodies and instruments.

An assay device, analytical instrument and assay method for determining the presence or amount of an analyte in a fluid is disclosed. The device is a one-step lateral flow dry reagent immunoassay with one, two or more zones along a transverse axis of the device, each zone can contain or be preceded by diffusively or non-diffusively bound reagents. The invention measures an indicator in one or two test zones for each analyte to determine its presence or concentration. The signal reagent (indicator) may be a particle such as a colored latex or colloidal gold. The assay quantitation may be read by an instrument.

A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed (typically for the presence of a particular protein), and a detection analyte (typically one or more antibodies bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre-incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.

The present invention relates to a composition for treating skin comprising an acylated short chain bioactive peptide and fulvic acid, and optionally colloidal gold. The invention further relates to a method for topically administering the composition in an amount therapeutically effective to reduce wrinkles by building the dermal fibroblast matrix. The invention further relates to a method of treating wrinkled skin by topically administering the composition to an individual in need of such treatment.

In the present invention, a method of producing stable bare colloidal gold nanoparticles is disclosed. The nanoparticles can subsequently be subjected to partial or full surface modification. The method comprises preparation of colloidal gold nanoparticles in a liquid by employing a top-down nanofabrication method using bulk gold as a source material. The surface modification of these nanoparticles is carried out by adding one or multiple types of ligands each containing functional groups which exhibit affinity for gold nanoparticle surfaces to produce the conjugates. Because of the high efficiency and excellent stability of the nanoparticles produced by this method, the fabricated gold nanoparticle conjugates can have surface coverage with functional ligands which can be tuned to be any percent value between 0 and 100%.

The present invention relates to polymeric materials that are labeled with colloidal metals, preferably colloidal gold, to processes for producing the labeled polymeric material, and to methods of using the materials in prophylactic, therapeutic and cosmetic applications. Specifically, the invention relates to porous injectable and implantable microparticles, preferably microspheres, that are associated with colloidal metals such that the microparticles are visible or detectable under regular light, by radiological and/or magnetic resonance imaging techniques, or both. The microparticles having colloidal metals are particularly useful for embolization, dermal augmentation and tissue bulking, drug delivery, gene therapy, and other prophylactic, therapeutic or cosmetic medical applications.

The invention relates to a nano gold biological composite probe, a detection method and application thereof. The detection method is characterized in that: the method comprises the following steps: firstly marking a bead through a monoclonal antibody of a protein to be tested; marking the polyclonal antibody of the protein to be tested on nano gold while and simultaneously marking a DNA probe with a biotin label; carrying out a biotin-streptavidin reaction on the DNA probe on the nano gold to make a lanthanide bonded with colloidal gold to construct the nano gold biological composite probe; mixing the bead of marked monoclonal antibody of the protein to be tested, the nano gold biological composite probe and a protein sample to be tested, carrying out the incubation on the mixture for a certain time at 37 DEG C; cleaning away the nano gold probe which does not react; adding a reinforcing liquid; and determining the fluorescence intensity so as to realize the aim of carrying out the quantitative determination on the protein to be tested. The method obviously improves the detection sensitivity of biomolecules, and is used for detecting a plurality of kinds of biomolecules synchronously. The method is widely applied in the fields of clinical diagnosis, antigen, antibody and nucleic acid detection, health quarantine, environmental tests, and the like.

This invention discloses a method for producing nanometer composite oxides. The procedures comprise: solubilise the molten substance in the mixture of hydrocarbon component and surfactantto form the molten salt-in-oil super solubilising colloidal group, which limits the hydroxide generated by the reaction of molten substance and precipitant in the colloidal group, so as to avoid the increase of particle size, and then obtain the nanometer composite oxides by burning.This invention is characterized of low cost of surfactant and hydrocarbon component, single distributed colloidal groups, narrow particle size distribution range and high yield. Besides, the process is simple and is adapted for industrial mass production. The nanometer composite oxide produced by this invention has large specific surface area and pore size, which makes it suitable for being used as catalyst carrier.

An apparatus (10) and method for use in a vertical flow-through assay process is characterised by applying pressure to a sample to force the sample through a reaction/capture membrane (12), to which one or more ligands are bound, at a controlled rate. Typically, the method includes a pre-incubation step in which the sample and a detection analyte typically an antibody bound to colloidal gold or a fluorescent tag bind together The pre-incubation step typically takes place in a chamber (26) spaced above the capture membrane (12). The base of the chamber is defined by a porous hydrophobic frit (34) typically formed from polyethylene. It is preferred that the chamber (26) is defined by the upper part of a cylinder (24) extending from a seal (30) compressing the reaction membrane (12) against an absorbent pad (28). The seal (30) has the effect of compressing tie reaction membrane and preventing wicking of the sample in the lateral direction outside of the circular seal. A piston (28) compresses air located in the chamber above atmospheric pressure to farce the sample to pass through the hydrophobic frit (34). Alternatively, a hydraulic actuator (60) may directly act on the sample to force the sample through the frit and reaction membrane at a predetermined rate.

The present invention discloses a multi-linked chromatographic test paper bar for detecting drug abuse in saliva and preparation thereof. The testing paper bar is a reaction film bar made of sample mats lap jointed mutually in turn pasted on the base, glass fiber film coated with colorful latex, colloidal gold or colloid selenium particles labeled protein, custodite film and absorbent paper. Control region coated anti-rat antibody, at least two detecting region of which coated with corresponding drug antigen are set on custodite film. The present invention has merits of safety and simple operation, suitable for detecting by single person/share and high detecting speed, and multi-detection of drug antigen in saliva at the same time.

The invention relates to a medical diagnostic reagent, in particular to a quick detection reagent for the early diagnosis of acute myocardial infarction and a test strip. The invention provides a double-index united detection reagent containing a specific antibody resisting a human heart-type fatty acid binding protein H-FABP and a human cardiac troponin cTnI. The invention also provides a colloidal gold labeling immunological chromatographic test strip containing the specific antibody resisting the H-FABP and the cTnI, which is used for quickly detecting the acute myocardial infarction. The double-index united detection reagent can carry out early diagnosis on a patient having the acute myocardial infarction, can also prevent the missed diagnosis of a patient having long-time chronic myocardial infarction with mild symptoms and better solves the influence caused by a difference existing in the detection time. The colloidal gold labeling immunological chromatographic test strip provides a quick, convenient, cheap and practical detection tool for the early diagnosis of the acute myocardial infarction, is hopefully used for hospitals at all levels and can also be used for the self monitoring of the patient.

The present invention relates to a superparamagnetic composite microparticle medicine-carrying body which can be mainly used for making magnetic target therapy and its preparation method. The invented magnetic composite microparticle medicine-carrying body is composed of magnetic composite microparticle and medicine-carrying layer which can be used for covering exterior of said magnetic composite microparticle. Said invention is characterized by that it utilizes the property of high surface activity of gold shell layer or colloidal gold of superparamagnetic composite microparticle to directly cover medicine or firstly utilizes the property of high surface activity of the gold shell layer or colloidal gold of superparamagnetic composite microparticle to make its surface be covered with high-molecular material, then utilizes the affinity adsorption or covalent linkage of high-molecular material to medicine to cover the medicine, also can make the surface of superparamagnetic composite microparticle be covered with the high-molecular material and medicine cross-linked or target preparation and medicine cross-linked or high-molecular material, target preparation and medicine cross-linked composite body layer.

Mesoporous ZSM compositions and methods of preparing same are provided. The starting material is an amorphous metallosilicate which is processed into ZSM-type nanocrystals. Upon calcinations, meso-ZSM-nanaocrystals are prepared.

The invention discloses an isothermal amplification kit for detecting SARS-COV-2 and a primer probe set. The kit comprises (1) inactivated lysate; and (2) an isothermal amplification system: a reaction buffer solution Buffer A, magnesium acetate Buffer B, negative control, nuclease-free water, a primer probe set and RAA enzyme. The lysate is used for rapid inactivation and release of viral nucleicacid, an isothermal amplification technology is utilized for rapidly enriching and amplifying a target area, whether a specific amplified product exists is rapidly determined through combination of aprobe with modification groups and a product with biotin, by a lateral chromatography technology and by use of colloidal gold developing. The kit is simple to operate, professional extraction reagents and detection instruments are not required limitation of detection laboratories and professionals is freed, the kit can perform rapid detection in an instrument-free state outdoors, the whole process only needs 30 min, and reading is very convenient.

The invention discloses test paper for rapidly detecting the immunochromatography of cadmium ion colloidal gold and a preparation method and application thereof. The test paper consists of a sample pad, a colloidal gold combination pad, a nitrocellulose membrane, absorbent paper and a plastic base plate; The sample pad, the colloidal gold combination pad, the nitrocellulose membrane and the absorbent paper are sequentially stuck on the plastic base plate; an anti-cadmium ion monoclonal colloidal gold label is coated on the colloidal gold combination pad; a goat anti-rat IgG antibody and a complete antigen Cd-iEDTA-BSA of heavy metal cadmium are sequentially coated on the nitrocellulose membrane; the goat anti-rat IgG antibody is used as a quality control line; and the complete antigen Cd-iEDTA-BSA of the heavy metal cadmium is used as a test line. The test paper detects residual metal cadmium in ambient soil, a water body and an aquatic product based on the immunology theory of antigen antibody; and compared with the prior detection system, the test paper has the advantages of rapidness, cheapness, convenience, sensitivity and speciality, can give a result within five minutes and is convenient to carry along to carry out field monitoring.