90 results about "Colloidal gold" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting feline coronavirus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 3C6 and 6H9, 3C6 serves as a coating antibody, 6H9 is used for colloidal gold labeling, and efficient and specific sandwich detection of feline coronavirus NP protein can be achieved. According to the invention, the monoclonal antibody combination is correspondingly applied to an immunodetection platform, and a rapid detection test strip or test paper card based on a colloidal gold immunochromatography technology is constructed. The test paper has high sensitivity to FECV-NP and FIBV-NP recombinant proteins, has no cross reaction with other proteins, and is suitable for rapid and on-site detection.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The embodiment of the utility model provides a nucleic acid extraction and colloidal gold detection all-in-one machine which is beneficial to reducing artificial participation and improving the working efficiency of nucleic acid extraction and detection. The nucleic acid extraction and colloidal gold detection all-in-one machine comprises a bottom plate; the reagent assembly and the sampling tube assembly are both arranged on the bottom plate, the sampling tube assembly is used for containing a sample, and the reagent assembly comprises a kit, a deep hole plate and a colloidal gold card box; the nucleic acid extraction assembly is movably connected to the bottom plate in the first direction and can stretch into the deep hole plate or be separated from the deep hole plate in the second direction, the second direction is the thickness direction of the bottom plate, and the first direction is perpendicular to the second direction; the liquid transferring assembly is configured to transfer liquid among the sampling tube assembly, the kit, the deep hole plate and the colloidal gold card box.

The disclosure provides a kind of recognition positioning method and system of colloidal gold particles in cryo-EM image, the scheme includes: obtaining the electron microscope image to be identified, and carries out corresponding pretreatment;Wavelet transform is handled to the image after pretreatment based on Atrous algorithm, obtains corresponding wavelet image;For the wavelet image obtained, in turn, binaryzation processing, noise point removal and clustering operation are carried out, obtain the corresponding several clusters of colloidal gold particles in binary image;From the several clusters, the cluster satisfying the preset constraint is screened out, and the average template is obtained based on the obtained cluster satisfying the preset constraint;Template matching is carried out using the average template and the several clusters respectively, and the cluster whose matching result is lower than the preset threshold is filtered out, and the remaining cluster is used as the candidate colloidal gold particle target;The position and size estimation of the candidate colloidal gold particle target obtained are obtained by corresponding the original image.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

The invention relates to the technical field of biological detection, in particular to a teletamine detection card as well as a preparation method and application thereof. The invention provides a tiletamine detection card. The tiletamine detection card comprises a bottom plate, a sample loading pad, a colloidal gold pad, an NC (nitrocellulose) membrane and a water absorption pad, a sample loading pad, a colloidal gold pad, an NC (nitrocellulose) membrane and a water absorption pad are sequentially arranged on the bottom plate along the liquid chromatography direction; the colloidal gold pad is coated with a colloidal gold labeled teletamine monoclonal antibody and a colloidal gold labeled first antibody; a detection line and a quality control line are sequentially arranged on the NC membrane along the liquid chromatography direction; the detection line is coated with a teletamine conjugate; the quality control line is coated with a second antibody; the second antibody can be specifically combined with the first antibody. The teletamine detection card and the colloidal gold detection kit provided by the invention have the advantages of good application effect, good specificity and good repeatability.

The application discloses a method for constructing a multiplex visual nucleic acid detection system, which comprises the following steps: S1, selecting specific conservative gene sequences of multiple target detection objects; S2, designing specific LAMP primers according to the specific conservative gene sequences of the multiple target detection objects selected in S1; S3, marking the specific loop primers designed in S2 and designing specific assimilation probes; S4, placing the primers, the marked primers, the assimilation probes and multiple nucleic acid templates formed in S2 and S3 into the same system for LAMP amplification to form amplification products containing multiple markers; S5, placing the amplification products containing multiple markers in S4 into a binding pad of a detection strip; and S6, under the action of chromatography, the amplification product-marked colloidal gold complex is captured by corresponding antibodies through a detection belt to realize color development. The application realizes double color development by using a visual LFD detection system, namely, UU and MH use different color development systems respectively, so that UU and MH can be rapidly and simply detected.

The present application relates to the technical field of immunochromatography, and in particular, to a test strip for detecting β-amyloid protein and the use thereof. In the present application, colored microspheres are conjugated to a β-amyloid monoclonal antibody via covalent bonds, and then the conjugate is coated onto a conjugate pad. Compared with colloidal gold, the colored microspheres exhibit greater stability and higher sensitivity. Additionally, the colored microspheres feature bright and diverse colors, uniform particle sizes, good monodispersity, and strong reproducibility of detection results. The test strip provided by the present application has the advantages of simplicity, rapidity and high timeliness, requires no additional reagents, instruments and professional personnel, and supports on-site operation. By means of simply applying a to-be-detected sample to a sample loading port of the test strip, a detection result can be interpreted within 15 min. The result interpretation is visual, intuitive, accurate and straightforward, with a low risk of human errors such as false positives and false negatives.

The invention discloses a universal probe for multiple nucleic acid detection, a probe combination and application of the universal probe and the probe combination, aiming at the limitation that the existing nucleic acid detection technology depends on expensive equipment, is complicated to operate and is difficult to realize multiple detection. A bridging detection system is formed by innovatively designing a general probe, a specific probe series A (two ends of which are fixed in sequence and are used for connecting the general probe and a quality control line) and a specific probe series B (one end of which is fixed on a detection line); the test strip structurally comprises a sample pad, a colloidal gold pad (for fixing a universal probe), an NC (nitrocellulose) membrane (containing a plurality of detection lines and quality control lines) and absorbent paper. The method is suitable for single-stranded nucleic acid (such as NASBA amplification products) detection, has high sensitivity, high specificity (no false positive), rapidness (completion within 10 minutes) and multiple detection capability, does not need complex instruments, is low in cost, and is easy to popularize in a basic level.

The application belongs to the field of biological detection, and particularly relates to a specific monoclonal antibody of an AKK bacterial efficacy protein Amuc_1100, a kit, a detection method and application thereof. The antibody pair is 3E10 and 2F7 monoclonal antibodies, the heavy chain and light chain variable region amino acid sequences of 3E10 are SEQ ID NO. 2 and SEQ ID NO. 4, and the heavy chain and light chain variable region amino acid sequences of 2F7 are SEQ ID NO. 6 and SEQ ID NO. 8. The antibody pair has high specificity and high affinity, a colloidal gold rapid detection kit and a double antibody sandwich ELISA quantitative detection kit based on the antibody pair realize rapid screening and accurate quantification of the Amuc_1100 protein, have good linearity, high precision and excellent accuracy, and can be used for detection of the protein in mucoid Akkermansia and quality control and activity evaluation of related probiotic preparations, thereby providing key technical support for related basic research and industrial application.

This invention relates to a method for the large-scale production of "I"-shaped test strips for hygiene products, belonging to the field of medical test strip processing. The "I"-shaped test strip is an improvement and breakthrough of conventional test strips, reducing the foreign body sensation and other discomfort caused to users when used in hygiene products without affecting the test strip's detection function. The large-scale production process includes cutting colloidal gold-nitrocellulose boards, attaching colloidal gold-nitrocellulose strips to a substrate, attaching sample pads and absorbent pads to the substrate, laminating a top waterproof membrane, and rolling the formed test strips. This process can continuously produce high-quality "I"-shaped test strip rolls, meeting the diverse production needs of the hygiene product industry.

The invention discloses a yeast immunochromatography rapid detection test strip and a preparation method thereof, and relates to the technical field of immunodetection.The test strip comprises a functionalized sample pad, a combination pad, a reaction film and a water absorption pad which are in lap joint in sequence; the sample pad is pretreated by a treating fluid containing beta-1, 3-glucanase, casein and TritonX-100, so that in-situ wall breaking and interference resistance can be realized; the reaction film detection line is coated with mannose binding lectin and a saccharomycetes specific monoclonal antibody, the binding pad fixes different epitope detection antibodies labeled by colloidal gold, the preparation method comprises the steps of functionalizing the sample pad, coating the reaction film, preparing the binding pad and assembling the test strip, and the matched kit comprises the test strip and a phosphate buffer diluent with a specific pH value. The method can realize extraction-free one-step rapid detection of saccharomycetes, has both specificity and stability, and is suitable for multi-scene screening.

The invention discloses a fracturing tracing monitoring method based on nano colloidal gold particles, and relates to the technical field of fracturing tracing. The nano colloidal gold particles prepared through special design serve as a tracer agent to be injected into a stratum along with fracturing fluid, a flowback signal is tested, and the fracturing tracing monitoring task which cannot be achieved by a common tracer agent at present is completed. The method comprises the implementation steps of tracer agent injection, flowback fluid sampling, tracer agent signal detection, curve drawing, fracture development degree evaluation and the like, and data support can be provided for fracture volume inversion, fracture complexity and fracture effect evaluation through implementation of the method. The method has the characteristics of rapid detection, accurate quantitative analysis, high detection sensitivity, accurate data, no background interference and the like, and has unique advantages in the field of fracturing tracing monitoring.

1. Name of the product in this design: Tester (Pen-shaped Colloidal Gold Tester). 2. Purpose of this design: To be used for the qualitative or quantitative detection and analysis of analytes in human samples using colloidal gold immunochromatography. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 1.

The application relates to the technical field of allergy detection, in particular to an inhaled allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit; the detection object is pollen, dust mites and animal dander; the kit comprises colloidal gold labeled mouse anti-human lgE antibodies arranged on a test strip combination pad; and pollen antigens (Art v1, Amb a1, Bet v2), dust mite antigens (Der f1, Der p1) and animal dander antigens (Can f1) arranged on a test strip nitrocellulose membrane. The kit provided by the application can quickly identify the allergen of inhaled allergic symptoms, classify the severity of allergic symptoms, and the identification result has a high accuracy.

The embodiment of the application belongs to the technical field of immunological detection in vitro diagnostic reagents, and relates to a preparation method of a detection card for detecting a novel coronavirus, comprising the following steps: preparing a gold nanorod solution; mixing the gold nanorod solution with a novel coronavirus monoclonal antibody to prepare a gold nanorod-antibody complex solution; preparing a binding pad and a sample pad through the gold nanorod-antibody complex solution; performing antibody coating on a nitrocellulose membrane to obtain a reaction pad coated with antibodies; assembling the binding pad, the sample pad and the coated membrane to obtain a colloidal gold immunochromatography test strip; and assembling the colloidal gold immunochromatography test strip and a shell to obtain the detection card. The application effectively improves the sensitivity of detection.

This invention discloses a blow-type sampling viral antigen detection kit and its usage method, including a sampler, a blow nozzle, a detection working column, and a equilibration liquid dropper. Sampling is performed by blowing air, a simple sampling method that reduces the probability of sampling errors in untrained individuals. Equilibration liquid is dripped into the funnel-shaped opening of the sampler's receiving tank through the equilibration liquid dropper, continuously eluting the biological sample. The operation is simple and effective. The equilibration liquid, along with the eluted biological sample, enters a colloidal gold filter. The colloidal gold is re-dissolved, captures and labels the viral antigen in the solution. The labeled antigen then enters an antibody filter impregnated with specific antibodies, where it is captured by those antibodies, forming a conjugate of antibody, antigen, and colloidal gold microparticles. A large number of these conjugate microparticles aggregate on the filter membrane above the absorbent cotton column, resulting in color development. The sample elution step is simple, the sampling method is reliable, and the detection and colorimetric observation are easy to perform, making it convenient for the general public to use.

The present application relates to the field of immunoassay technology, discloses a product for early diagnosis of sepsis and its application, the composition of the product for early diagnosis of sepsis includes detecting SAA1 protein and PDL1 + The product for detecting the content of exosome in sample is based on antigen-antibody specific immune binding to detect SAA1 protein and PDL1 + The product for detecting the content of exosome in sample, the detection method adopted by the detection product is selected from one of fluorescence immunoassay, enzyme-linked immunosorbent assay, colloidal gold immunochromatography, chemiluminescence immunoassay, electrochemical immunoassay and nanopore-based immunosensing detection method, the product for early diagnosis of sepsis improves the accuracy of early diagnosis of sepsis and disease condition evaluation in a double-index cooperative manner, effectively solves the technical bottleneck that the existing sepsis detection method is insufficient in sensitivity, poor in specificity and difficult to meet the requirements of early precise diagnosis and dynamic disease condition evaluation.

This invention discloses a nanoprobe for detecting thimerophos and an immunochromatographic method for detecting thimerophos. The nanoprobe for detecting thimerophos comprises a labeled antibody complex. This invention utilizes the reaction of dopamine with the thiol group of 4-MBA under alkaline conditions to polymerize a large amount of 4-MBA, providing a stronger SERS signal. Dopamine itself possesses a strong photothermal effect, enhancing the detection signal and thus increasing sensitivity. By adding a TEOS-coated silica shell, the coupling rate with the target antibody is increased, improving the inhibition rate of the immunochromatographic strip. The detection limit for thimerophos using micro Raman spectroscopy is 20.42 ng / mL, and the detection limit using an 808 near-infrared spectroscopy instrument is 23.53 ng / mL. The detection limit of the traditional colloidal gold immunochromatographic method is 82.49 ng / mL. Compared with the traditional colloidal gold immunochromatographic method, the multimodal high-sensitivity immunochromatographic technique using core-shell nanoprobes improves the sensitivity of thimerophos detection by approximately four times.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

The invention belongs to the field of biological detection, and particularly relates to a specific monoclonal antibody of AKK bacterium functional protein Amuc1100, a kit and a detection method and application thereof. The antibody pair is a 3E10 monoclonal antibody and a 2F7 monoclonal antibody, amino acid sequences of variable regions of a heavy chain and a light chain of the 3E10 are SEQ ID NO.2 and SEQ ID NO.4, and amino acid sequences of variable regions of a light chain of the 3E10 are SEQ ID NO.6 and SEQ ID NO.8. The antibody is strong in pairing specificity and high in affinity, colloidal gold rapid detection and a double-antibody sandwich ELISA quantitative detection kit constructed based on the antibody respectively realize rapid screening and accurate quantification of Amuc1100 protein, the detection linearity is good, the precision is high, and the accuracy is excellent; the method can be used for protein detection in mucus Ackerman bacteria and quality control and activity evaluation of related probiotic preparations, and provides key technical support for related basic research and industrial application.

This utility model discloses a rapid detection kit for thiamethoxam colloidal gold, comprising a main body, a fixing mechanism, an installation mechanism, and a closing mechanism. The fixing mechanism includes a shelf and a reset plate, the installation mechanism includes a test plate and test tubes, and the closing mechanism includes a sealing gasket and a closing cap. With the cooperation of the above mechanisms, this design of the device helps to maintain the orderly storage of the multiple internal components of the kit, reducing the possibility of confusion and erroneous operation. Users can easily find the required test boxes, test tubes, and reagent bottles, improving work efficiency. In addition, this device can fix the components inside the main body, making it convenient to carry the components inside the main body.

The utility model provides a colloidal gold detection kit with an anti-pollution structure, and relates to the field of kits. The colloidal gold detection kit with the anti-pollution structure comprises a lower kit body, an upper kit body is installed on the top of the lower kit body, detection test paper is arranged in the lower kit body, a display opening is formed in the top of the upper kit body in a penetrating mode, the inner wall of the display opening is fixedly connected with glass, and the inner wall of the display opening is fixedly connected with the anti-pollution structure. A sampling opening is formed in the top of the upper kit in a penetrating manner, a groove is formed in the top of the upper kit, a damping bearing is fixedly connected to the inner wall of the groove, a rotating cover is fixedly connected to the top of the damping bearing, and a groove is formed in the top of the rotating cover. The colloidal gold detection kit with the anti-pollution structure, provided by the utility model, has the advantages of moisture prevention and oxidation prevention.

This invention discloses a highly specific and sensitive immunochromatographic test strip, comprising a first conjugation pad, a second conjugation pad, a nitrocellulose membrane coated with C and T lines, an absorption pad, and a base plate. The first conjugation pad is immobilized with antibody A conjugated to oxidized dextran, representing the antigen to be detected. The second conjugation pad is immobilized with ConA-conjugated colloidal gold and anti-DNP antibody conjugated to colloidal gold. The T line is coated with antibody B, representing the antigen to be detected. The C line is coated with DNP. The invention also discloses the preparation method of the test strip, the detection kit, and the detection method. This invention introduces the dextran and ConA system. ConA is a tetrameric globulin, with each subunit containing one sugar-binding site, and each ConA having four sugar-binding sites, thereby enhancing the detection signal, amplifying the signal, and improving detection sensitivity. It is suitable for the detection of various product types and has broad application prospects.

The application provides a multifunctional full-automatic colloidal gold detector, which comprises a feeding mechanism, an X-axis sample adding mechanism, a TIP conveying mechanism, an incubation mechanism, a detector mounting table and a control device, a sample tube is mounted on the feeding mechanism, the feeding mechanism can add diluent into the sample tube and mix to form a sample to be used, a reagent card is placed on the incubation mechanism, and a TIP head is arranged on the TIP conveying mechanism; a TIP needle rod is arranged on the X-axis sample adding mechanism, the X-axis sample adding mechanism can install the TIP head on the TIP needle rod and can take the sample from the sample tube through the TIP head and drop the sample on the reagent card, the reagent card to which the sample is dropped is transported to an incubation bin for incubation, and detection is completed in the incubation mechanism after the incubation is completed, the detector is integrated into a full-automatic device, errors in manual operation are avoided, the workload of doctors is reduced, the detection accuracy is improved, and the detection efficiency is greatly improved.

The invention discloses a detection kit for detecting the content of calprotectin in synovial fluid. The detection kit comprises a detection kit shell and a calprotectin immunochromatography test strip, and the calprotectin immunochromatography test strip is arranged in the detection kit shell; the calprotectin immunochromatography test strip comprises a sample pad, a glass fiber combination pad, a coated nitrocellulose membrane and a water absorption pad which are bonded in sequence, an anti-calprotectin antibody marked by colloidal gold is embedded in the glass fiber combination pad and is used for being combined with calprotectin in a sample to be detected to form a compound; the anti-calprotectin monoclonal antibody is coated on a detection line of the nitrocellulose membrane, and the anti-mouse IgG antibody is coated on a quality control line of the nitrocellulose membrane; the colloidal gold labeled anti-calprotectin antibody is embedded in the glass fiber cushion layer, and the image recognition module is used for collecting a tested test paper image and calculating a relative gray value of a detection line and a quality control line, so that a calprotectin concentration interval is judged.

The utility model discloses a bloodstain screening device, which relates to the field of bloodstain screening and comprises a screening component, the screening component comprises a hollow through shell, a reagent strip limiting tube is fixedly connected inside the front end of the through shell, and the front end of the through shell is gradually narrowed flat. The bloodstain screening device comprises a through shell, a colloidal gold reagent strip is arranged in the through shell, a reagent strip limiting tube is arranged in the through shell, the reagent strip limiting tube is of a flat strip-shaped design with the hollow interior, the through shell and the reagent strip limiting tube are both made of transparent acrylic materials, and a test tube is fixed in the middle of the interior of the through shell. The internal buffer solution can fully soak the reagent strip conveniently, the internal buffer solution is absorbed by the built-in cotton ball, the internal piston rod is pushed to extrude the cotton ball when inspection is needed, the buffer solution is extruded out to be in contact with the reagent strip, the buffer solution does not need to be prepared in real time during detection, and the overall detection efficiency can be improved.

The invention relates to the technical field of oil-control shampoo, and particularly discloses a test strip for early screening of HPV nucleic acid and a preparation method thereof.The test strip comprises a PVC backboard, a sample pad and a water absorption pad are arranged at the two ends of the top of the PVC backboard respectively, an NC film is arranged in the middle of the top of the PVC backboard, one side of the top of the NC film is in extrusion contact with the bottom of the water absorption pad, and the other side of the top of the NC film is in extrusion contact with the bottom of the water absorption pad; a combination pad is arranged on the other side of the top of the NC membrane in an extrusion contact manner, and one side of the top of the combination pad is in extrusion contact with the bottom of the sample pad. The invention provides a test strip for early screening of HPV (human papilloma virus), which aims at HPV nucleic acid and is developed on the basis of a colloidal gold technology. The test strip is used for solving the problems of complicated operation, dependence on professional instruments and personnel, high detection cost, incapability of meeting the requirement on convenience of early screening and the like in the existing HPV detection technology. Through the method, rapid and accurate detection of the HPV can be realized, and the method is suitable for large-scale early screening including scenes such as home self-inspection and the like.

The invention belongs to the technical field of biological detection, and particularly relates to a dual MIRA detection method for type A clostridium botulinum and staphylococcus aureus for a portable reagent card incubator. The double colloidal gold type MIRA method established by the invention has good specificity on type A clostridium botulinum and staphylococcus aureus, and has no cross reaction with six strains such as pasteurella multocida, klebsiella pneumoniae and salmonella. The lowest detection limits of the method on the staphylococcus aureus and the A-type clostridium botulinum are 2.94 * 100 copies / mu L and 2.27 * 100 copies / mu L respectively. The optimized double colloidal gold type MIRA method can be successfully applied to a reagent card incubator. The detection method disclosed by the invention has the advantages of good specificity, high sensitivity, simplicity in operation, short time consumption and the like.