143results about How to "Quantitative results are accurate" patented technology

An assay device, analytical instrument and assay method for determining the presence or amount of an analyte in a fluid is disclosed. The device is a one-step lateral flow dry reagent immunoassay with one, two or more zones along a transverse axis of the device, each zone can contain or be preceded by diffusively or non-diffusively bound reagents. The invention measures an indicator in one or two test zones for each analyte to determine its presence or concentration. The signal reagent (indicator) may be a particle such as a colored latex or colloidal gold. The assay quantitation may be read by an instrument.

The disclosure relates to a dry reagent assay device having two or more test zones for detecting two or more analytes in a test sample. In one embodiment, the test zones may be the site of a binding reaction between members of a binding pair, or they may be the site of a general chemistry reaction. The disclosure also relates to methods for performing assays to detect analytes in test samples using such devices.

The invention relates to a method for simultaneously measuring residual quantities of lincomycin and macrolides in royal jelly by a liquid chromatography and a tandem mass spectrometer. Currently, the prior method can not achieve the goals of low detection cost and a detection limit less than 1 mu g / kg. The method comprises the steps of standard solution preparation, royal jelly sample treatment,standard curve making and royal jelly sample detection; in the step of royal jelly sample treatment, acetonitrile ammonia water with the volume concentration of 97% is used as the extracting solution, residues in a 50 ml glass centrifuge bottle after vacuum concentration are transferred by a phosphate buffer solution and mixed with ethyl acetate so as to carry out extraction; and finally, the detection sample solution is measured by a high performance liquid chromatograph and a ternary quadrupole tandem mass spectrometer to obtain the residual quantities of lincomycin and macrolides in royal jelly. The invention has the advantages of low detection cost and high detection efficiency, the detection limit reaches 0.2-0.9 mu g / kg, and the linearly related coefficient is 0.9914-0.9994.

The invention discloses a method for quickly detecting a drug. The method comprises the following steps of: (1) pretreating a sample; (2) performing gas chromatography separation and mass spectrum scan analysis on the sample; (3) performing extract ion analysis on each chromatographic peak on a total ion chromatogram by using a mass spectrum characteristic ion of a common drug and initially judging a suspected drug component; (4) drawing an extract multi-ions figure of the chromatographic peaks by using the characteristic ion of the suspected drug component, calculating the abundance ratio of the characteristic ion and determining whether the drug component exists in the sample or not; and (5) performing gas chromatography-mass spectrum analysis on a standard substance of the suspected drug component and measuring the content of the drug by adopting an external standard method. By adopting the method, a qualitative result is reliable and a quantitative result is correct. Meanwhile, a plurality of drugs can be detected at the same time, so that the efficiency is greatly improved and the detection cost is lowered.

The invention discloses a method for screening pesticide and veterinary drugs in infant formula food by ultra-high performance liquid chromatography-quadrupole static electric field track ion trap mass spectrum, and belongs to the technical field of food safety inspection. The method comprises the following steps: preprocessing a sample by an optimized QuEChERS method; screening by applying ultra-high performance liquid chromatography-quadrupole static electric field track ion trap mass spectrum; selecting a positive ion or negative ion mode for scanning by adopting full-scan / data dependence acquisition, full-scan / variable data independence acquisition and full-scanning / full-ionic acquisition fragmentation acquisition scan modes to obtain complete primary and secondary maps; and processing the primary and secondary maps by applying Exact Finder 2.5 software to extract compound information. According to the method, information in the established spectrum library is compared with the screening result of a to-be inspected object so as to ensure the composition of pesticide and veterinary drugs in an infant formula food. The mass spectrum instrument used in the method has the main advantages of high resolution ratio, accurate qualitative and quantitative results, high sensitivity, high quality precision and the like. The method can be applied to analysis of complex substrates.

The invention belongs to the technical field of food detection, and particularly relates to a fast instrumental analysis method for aflatoxins in foods. The invention discloses a fast instrumental analysis method for aflatoxins B1, B2, G1 and G2 in foods. The method comprises the following steps of: weighing a certain amount of sample; adding an acetonitrile-water solution in a certain ratio; performing ultrasonic extraction; and selecting specific parent ions and daughter ions by using a super-efficient liquid phase chromatography-mass spectrometer under a gradient elution condition to measure the content of the four types of aflatoxins. The method is simple and is high in efficiency and the precision of measurement results is high. By the method, four monomers of the aflatoxins are completely separated, and the recovery rate is high; and compared with the prior art, the method has the advantages of high speed and accuracy of detection and simple pretreatment, and can be used for accurately determining the nature and the quantity to meet requirements on research and detection.

The invention discloses a method for detecting content of short-chain or medium-chain chlorinated paraffin in a paint. The method comprises the following steps: extracting the short-chain or medium-chain chlorinated paraffin in the paint, detecting with a gas chromatography-negative chemical ionization source mass spectrometry detector, using the internal standard method, performing linear association on a total response factor of a short-chain or medium-chain chlorinated paraffin standard sample on the NCI (negative chemical ionization) mass spectrometry detector with the chlorine content of a short-chain or medium-chain chlorinated paraffin standard solution, detecting the actually measured chlorine content of the sample by the gas chromatography-negative chemical ionization source mass spectrometry detector, corresponding the actually measured chlorine content with the a liner reaction expression of the total response factor and the chlorine content of the short-chain or medium-chain, to obtain the total response factor of the sample, and thus obtaining the content of the short-chain or medium-chain chlorinated paraffin in the paint. The analysis method disclosed by the invention is exact and fast, has small interfering, and can perform optimal separation and qualitative and quantitative detections on each component in the sample.

The invention relates to a method for determining the total nitrogen content in a nitrate-nitrogen-containing compound fertilizer through a post-distillation titration method. According to the invention, contents of nitrogen of different forms, including Kjeldahl nitrogen, nitrate nitrogen and ammonium nitrogen, are determined respectively; and total nitrogen content is then calculated. The determination method of the total nitrogen content in nitrate-nitrogen-containing compound fertilizer is accurate and reliable, and a relative average deviation is smaller than 10%. Therefore, the method provided by the invention provides a reliable and easy-to-practice method for detecting total nitrogen content in nitrate-nitrogen-containing compound fertilizer, such that research and production requirements can be satisfied.

The invention provides a quantitative analysis method for volatile components in liquid products of a thermal-simulation hydrocarbon generation experiment. The quantitative analysis method comprises the following steps of collecting and treating the liquid products of the thermal-simulation hydrocarbon generation experiment, specifically, collecting, removing water, removing slag and injecting a standard sample; quantitatively analyzing the volatile components in the liquid products of the thermal-simulation hydrocarbon generation experiment through a comprehensive two-dimensional gas chromatography-hydrogen flame ionization detector, specifically, carrying out primary comprehensive two-dimensional analysis, volatilizing to reach constant weight and secondary comprehensive two-dimensional analysis; calculating a quantification result of the volatile components in the liquid products of the thermal-simulation hydrocarbon generation experiment. The quantification result of the volatile components in the liquid products of the thermal-simulation hydrocarbon generation experiment, which is obtained by the quantitative analysis method disclosed by the invention, is real, reliable and high in repetitiveness, so that a reliable technical support is supplied to evaluation of an oil-gas resource amount.

The invention discloses a determination method for an austenitic grain size of a pearlite type wire rod. The determination method comprises the following steps: (1) cutting off a small block from the pearlite type wire rod to be used as a test sample; polishing and mechanically burnishing, and then carrying out electrolytic polishing; (2) taking one face of the test sample as an observation region, and carrying out face scanning on the test sample through an EBSD (Electron Backscatter Diffraction) system to obtain an orientation imaging picture; and (3) setting a deflection angle difference of 25 degrees, and counting up the grain size through orientation analysis software of the EBSD system to obtain an average grain size diameter, and obtaining the austenitic grain size of the test sample according to an average grain size diameter comparison table 1. According to the method disclosed by the invention, an accurate quantization effect can be obtained according to parameters of a standard sample preparation process, the repeatability of the result is good and the success rate is high; and the method can clearly present tissue uniformity of the wire rod, also can be matched with a traditional austenitic grain size index and is easy to understand.

The invention relates to a proteome label-free quantification method combining tandem mass spectrometry with machine learning algorithm, which is used for absolute and relative quantitative analysis of a proteome level. The method firstly analyzes an enzymolytic peptide fragment mixture of a proteome actual sample for establishing a training data set and an enzymolytic peptide fragment mixture of a proteome sample to be analyzed by using a liquid chromatography-tandem mass spectrometry system. The total amount of the samples can be obtained by cell counting or protein concentration determination, and the absolute amount of each protein can be calculated according to the percents and sample total amount calculated in the last step. The absolute amounts of the same protein in different samples are compared to obtain relative quantitative information of the protein in different samples. The method has good accuracy in both absolute quantification and relative quantification.

The invention provides a monitoring method for corrosive damage of the hole edge of an aluminum alloy porous structure based on an iterative algebraic reconstruction algorithm. The monitoring method comprises the following steps: firstly, selecting a porous aluminum alloy, and arranging piezoelectric sensors by a square array layout; secondly, adopting a sine wave exciting signal; thirdly, imaging the damage by applying a tomography scanning algorithm of ART according to the network layout of the piezoelectric sensors and selected signal characteristic quantity; fourthly, carrying out location imaging on the damage by analyzing a correlation coefficient of Lamb waves; fifthly, adopting mean filtering processing, and solving a value of a final pixel by using a mean of a template to replace a value of an original pixel. According to the monitoring method disclosed by the invention, by performing the steps, different piezoelectric sensors are acquired by layout optimization of the piezoelectric sensors and signal monitoring; characteristic parameters are extracted by signal processing, the corrosive damage degree of a porous aluminum alloy member can be quantitatively characterized, and the function of monitoring the corrosive damage of the aluminum alloy structure in real time can be realized.

The invention provides a method for measuring the content of wood preservatives of wooden products for children. The method comprises the following steps: crushing a sample to be measured, performing ultrasonic extraction by using methanol, filtering, adding an interior label working solution into the filtrate, centrifuging, filtering the supernate by using a filtering membrane, and performing spin-steaming concentration; and adding a potassium carbonate solution into the concentrated liquid, shaking uniformly, adding acetic anhydride, performing derivatization, oscillating while deflating and allowing the derivatized concentrated liquid to pass through an Oasis hydrophile lipophile balance (HLB) solid phase extraction column; sequentially leaching by using deionized water, pumping all the water by using a vacuum pump and eluting by using ethyl acetate to obtain eluent; adding a proper amount of anhydrous sodium sulfate into the eluent and drying; and performing vortical oscillation and measuring by a gas chromatography-tandem mass spectrum method. The invention establishes the method for measuring the content of 10 wood preservatives of the wooden products for children based on solid phase extraction-gas chromatography-tandem mass spectrum, which has excellent selectivity and sensitivity on detection of target objects of wood samples with complex matrix.

The invention relates to a method for quantitatively evaluating each phase structure in hot rolling TRIP (transformation-induced plasticity) steel. The method comprises the following steps: quantitatively measuring an FCC phase (retained austenite) and a BCC phase (ferrite, bainite and martensite) of the hot rolling TRIP steel by using an electronic backscattered diffraction device arranged on a scanning electron microscope; firstly recognizing the retained austenite according to a distinct difference of the FCC phase and the BCC phase in structure, and then quantitatively distinguishing the three phases by using a gray value difference in a kikuchi band contrast BC (Band Contrast) image in EBSD (electron backscattered diffraction) processing software according to the difference of the three similar structure phases of the ferrite, the bainite and the martensite in kikuchi pattern quality, so as to achieve multi-phase separation and quantitative analysis of the hot rolling TRIP steel. The method is accurate and reliable, and can be applied to the technical field of measurement of tissues in the steel.

The invention provides a method for measuring isothiazolinone antibacterial finishing agents in textiles, and relates to the field of product quality analysis, and discloses a method for separating and detecting the five isothiazolinone antibacterial finishing agents. The following liquid phase chromatographic conditions are adopted, and according to the liquid phase chromatographic conditions, a chromatographic column is XR-ODS (2.2 microns), the inner diameter is 100 mm*3.0 mm, column temperature is 40 DEG C, column flow speed is 0.4ml / min, a diode array detector is adopted, and detection wavelengths are 277 nm and 283 nm. According to the gradient elution conditions, the volume ratio of methanol to water is 40 to 60 for 0.00 min; the volume ratio of methanol to water is 90 to 10 for 1.90 min; the volume ratio of methanol to water is 90 to 10 for 6.00 min; the volume ratio of methanol to water is 40 to 60 for 6.01 min; 9.00 min. The five isothiazolinone antibacterial finishing agents are separated, a good baseline separation effect can be achieved, and peak types are sharp and symmetric.

The invention relates to a detection method of halogenated alkyl phosphate and includes the following steps: samples are pre-treated with a Soxhlet extraction method or a supersonic extraction method; a high-performance liquid phase color spectrometer which connects diode array detectors is used for detecting the halogenated alkyl phosphate, and the proportions of the organic flow phases of the spectrometer are set from 50 to 100 percent, and the proportions of the inorganic flow phases are set from 0 to 50 percent, and the flow rates are set from 0.5 to 1.0ml per minute and the injection volume is set from 10 to 40ul and an anti-phase chromatographic column is selected as the chromatographic column; the samples pre-treated are brought into the chromatographic column by the flow phases of the high performance liquid phase color spectrometer, and the temperature of a column temperature box is set from 20 to 40 DEG C and components of the samples are separated from the chromatographic column; the components of the samples separated by the chromatographic column enter the diode array detectors to be detected. The detection method of the invention can prevent the samples from decomposing and is accurate and secure in analysis method.

The invention discloses a convenient ATP (adenosine triphosphate) content detecting method adopting pressure-sensitive paint as a signal reading tool. Two fragments of DNA capable of forming an ATP aptamer are modified with a silica sphere with embedded nano platinum particles and a magnetic bead with embedded nano platinum particles respectively. In the presence of ATP, a sandwich structure can be formed due to the specific recognition and combination of the two fragments of DNA and ATP, and accordingly, nano platinum is fixed on the magnetic bead; the magnetic bead is separated from a solution, the cleaned magnetic bead is subjected to a reaction with a hydrogen peroxide solution in a closed container, oxygen partial pressure can be increased through accumulation of oxygen generated by catalysis of hydrogen peroxide, and the concentration of ATP is indirectly detected according to fluorescence quenching degrees of different oxygen partial pressure to the pressure-sensitive paint. The technology is successfully applied to the detection of ATP concentration in human body serum; according to the detection, a fluorospectrophotometer is adopted as a detecting means, and naked eyes can also be directly used for colorimetric detection; the method has the advantages of being low in detection cost, easy to operate, accurate in quantitative result, capable of realizing instant detection, convenient and the like.

The invention, which belongs to the technical field of tobacco component detection, relates to an analytical method for quantitatively measuring super-multi-target aroma components in tobacco. The method comprises the following steps that: a tobacco sample is immersed in an acidic buffer solution and extraction is performed by an organic solvent; a multi-walled carbon nanotube is used as a reversesolid phase-dispersed adsorbent and vortexing and centrifugation are performed to purify the sample; and then simultaneous detection of 345 kinds of aroma component in tobacco is realized by combining gas chromatography-tandem mass spectrometry. The method has advantages of simple and rapid operation, high flux, low cost, low solvent dosage, good environmental friendly effect, wide range of analytical compounds, high accuracy, high precision, high sensitivity and high repeatability and can satisfy the demand of rapid detection and analysis of aroma component in tobacco.

The invention relates to a method for detecting organic matters in soil through a potassium dichromate oxidation process, which comprises the steps of weighing a test sample, adding 5mL of 0.9 to 1.1mol / L of potassium dichromate standard solution and 5mL of concentrated sulfuric acid, and heating the test sample in a boiling water bath for 35 to 40 min; completely absorbing the cooled mixture into a conical flask with 250mL, adding a phenanthroline hydrate indicator, and using a ferrous sulfate solution to titrate to an end point. The method for detecting the organic matters in the soil through the potassium dichromate oxidation process optimizes the concentration of the potassium dichromate standard solution and the influence of the heating temperature and time on a detection result. The method provided by the invention is adopted to detect the content of the organic matters in the soil accurately and reliably, and the relative mean variation is less than 10%. Thus, according to the method provided by the invention for detecting the contents of the organic maters in the soil, a method which is reliable and convenient to realize is provided to meet the requirements in research and production.

The invention discloses an automobile dynamic property and economy expectation quantification method, which belongs to the technical field of driving intention identification. The method is divided into two stages of offline modeling and real-time quantification. In the off-line modeling stage, the automobile road test speed, the accelerator opening degree and the dynamic property expectation subjective evaluation of different drivers are collected and processed; a fuzzy neural network is adopted to establish a driver dynamic property expectation quantification model; and a driving manipulation characteristic parameter and vehicle operation state parameter prediction model are established based on the exponential smoothing-Markov model. In the real-time quantification stage, the vehicle speed and the accelerator opening degree are collected in real time; parameter prediction is carried out through a driving manipulation characteristic parameter and vehicle running state parameter prediction model established offline; the predicted value is input into a driver dynamic property expectation quantification model to obtain a dynamic property expectation value; and the expected economicvalue is further calculated. The method can continuously quantify the expectation of the driver for the dynamic property and economy of the automobile, and has the characteristic of high quantizationprecision.

A fluorescence quantitative PCR reagent kit for detecting the foot-and-mouth disease virus features that the fluorescent probe (TaqMan) and the positive-sensor or antisence primer are used, and the in vitro transcripted RNA standard is directly used as the reference for both reverse transcription and amplification. It can be used for detecting the monitoring the foot-and-mouth disease and detecting the safety of animal and its food.

The invention provides a dried blood spot quantitative collection device. The dried blood spot quantitative collection device comprises a sample collection tube, sample collection filter paper and a quantitative blood collection tube, wherein the sample collection tube comprises a tube body and a tube cover; the tube body is a straight tube, and the tube cover is used for sealing the tube opening.The quantitative blood collection tube comprises a quantitative capillary tube and a fixing piece; the quantitative capillary tube is fixed on the fixing piece and is provided with a blood sampling end which is used for sampling blood and is exposed out of the fixing piece; the fixing piece is clamped in the tube opening, and the quantitative capillary tube extends into the tube body; the samplecollection filter paper is arranged in the tube body, and the blood sampling end abuts against the sample collection filter paper. The invention further provides a method for collecting the dried blood spots. The integrated blood collecting device design is adopted, sample collection, drying, transportation, storage and extraction can be achieved in the same device, sample pollution is avoided, and the accuracy of a dried blood spot quantitative detection result is improved.

The invention relates to a detection method for 2-methoxyethanol, 2-ethoxyethanol, 2-methoxyethanol acetate and 2-ethoxyethanol acetate, comprising the following steps: pretreating a sample with Soxhlet<,>s extraction method or ultrasound extraction method; detecting 2-methoxyethanol, 2-ethoxyethanol, 2-methoxyethanol acetate and 2-ethoxyethanol acetate with a gas chromatograph providing with a hydrogen flame ionization detector, with the injector temperature of 180-250 DEG C, high purity nitrogen as carrier gas, the carrier gas flow rate of 0.8-1.5ml / min, and polar column as chromatographic column; bringing the pretreated sample in the chromatographic column through the gas chromatograph for programming temperature from 40-80 DEG C to 220-240 DEG C; and separating the four objects from the chromatographic column. The method has the advantages of cheap detector, simple and safe operation, accurate and rapid analytical method.

The invention discloses an ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy products. The method is characterized in that a sample is pre-treated by an optimized QuEChERS method; a ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry is used for screening, full-scanning / data-independent collection, full-scanning / variable data non-independent collection, and full-scanning / ion fragmentation collection scanning mode is employed, a positive ion or negative ion mode is selected for scanning to obtain complete first-order and second-order spectrums; Exact Finder 2.5 software is used for processing the obtained first-order and second-order spectrums, and the compound information is extracted. Compared the information in spectrum database and the screening result of the to-be-measured objects, composition of mycotoxin in the milk and dairy products can be confirmed. The mass spectrum apparatus has the advantages of high resolution, accurate qualitative and quantification result, high sensitivity, and high quality precision, and can be used for analyzing the complex matrix.

The invention belongs to the technical field of natural product detection, and relates to a quantitative detection method of UPLC-PDA-MS / MS of triterpenoid saponins in Camellia sinensis (L.) O.Kuntze.The UPLC-UV-QTOF-MS / MS method of quantitative determination of the triterpenoid saponins in the Camellia sinensis (L.) O.Kuntze comprises the following steps that (1) samples to be detected are pretreated; (2) any one or more saponin compounds known in the Camellia sinensis (L.) O.Kuntze are taken, and a standard product solution is prepared; and (3) the structure of high performance liquid chromatography / ultraviolet / mass spectrometry combination equipment (UPLC-PDA-QTOF-MS / MS) of a test solution and a standardized product solution is identified by tandem mass spectrometry, and an external standard method is used for quantitative detection under a PDA detector. According to the UPLC-UV-QTF-MS / MS method of quantitative detection of the triterpenoid saponins in the Camellia sinensis (L.) O.Kuntze, quantitative results are more accurate; the sensitivity is high; and the sample demand amount is less.

The invention discloses a method for detecting the content of isosuccinic acid in blood or urine. The method comprises the following steps of: respectively uniformly mixing a standard sample and a sample to be detected with an internal standard solution, adding a matrix solution and uniformly mixing; adding an extracting reagent and mixing uniformly and then centrifuging; taking supernatant and blow-drying; adding saturated acidic alcohol solvent, mixing uniformly, sealing and heating to 65 DEG C and derivatizing for 20 min; blow-drying the solvent after the derivatization is completed; adding mobile phase redissolving liquid for redissolving and mixing uniformly; carrying out ultrasonic extraction, mixing uniformly and centrifuging; taking supernatant and enabling the supernatant and the mobile phase to pass through a chromatographic column for liquid-mass spectrum analysis so as to finally detect the content of isosuccinic acid in blood or urine. The method can be used for detecting the content of isosuccinic acid simply, conveniently, quickly and accurately, can be applied to mass sample screening and has the advantages of accurate quantification and strong specificity.

The invention discloses a fuzzy theory-based process failure mode identification and evaluation method, which belongs to the field of machining and can be popularized to the field of improvement of machining quality of other products. The method comprises the following steps of solving a failure judgment matrix in combination with a process failure judgment criterion based on process element decomposition, thereby realizing identification of process failure modes; obtaining a fuzzy evaluation matrix by virtue of an expert system and a fuzzy evaluation level; based on an analytic hierarchy process of a fuzzy theory, calculating subjective weights of evaluation indexes, calculating objective weights of the evaluation indexes by an entropy weight method, and calculating weights of the evaluation indexes in combination with the subjective weights and the objective weights; obtaining a sequence of the process failure modes by a TOPSIS (technique for order preference by grey similarity to ideal solution) method; in combination with the failure mode sequence, grading the process failure modes by a rank sum ratio method; and performing product machining process improvement on processes ofthe process failure modes with the relatively high grade, thereby improving the product machining quality and reliability.

The invention provides a method for detecting the smoke composition of a cigarette which is noncombustible under heating. The method comprises the following steps: (1) preparation of an internal-standard solution; (2) preparation of a standard working solution; (3) smoking of the cigarette noncombustible under heating, and collection of total particulate matters via a Cambridge filter; (4) the extraction of the total particulate matters; (5) gas chromatographic determination; and (6) calculation of determination results by using a double-internal-standard method. The method established in theinvention utilizes a traditional cigarette smoking machine for smoking of the cigarette noncombustible under heating and capturing of the total particulate matters and does not need a special smokingmachine for the cigarette noncombustible under heating; the method realizes simultaneous analysis and detection of six target components in one experiment; the method utilizes double internal standards for quantitative determination, so quantification results are more accurate; sample smoking and the capturing and extraction of the total particle particulate matters are simple to operate; and thegas chromatographic determination is convenient and stable, and has the advantages of good linear correlation, good practicality, high sensitivity, good repeatability and the like.