47 results about "Methylmalonic acid" patented technology

The invention relates to the detection of methylmalonic acid (MMA). In a particular aspect, the invention relates to methods for detecting derivatized methylmalonic acid (MMA) by mass spectrometry.

The invention relates to the technical field of agricultural microbiology, and specifically relates to an application of methylmalonic acid in preparation of a nematode pesticide. Through determination of the fatality rate of meloidogyne incognita and caenorhabditis elegans, it finds that the methylmalonic acid has a good insecticidal effect on nematodes; LC50 of the methylmalonic acid to the meloidogyne incognita and the caenorhabditis elegans is separately 13.11 and 1.20; and after the methylmalonic acid and betaine are compounded, the LC50 of the methylmalonic acid to the meloidogyne incognita and the caenorhabditis elegans is separately 2.85 and 0.27; and at the same time, the methylmalonic acid has an inhibition effect on ralstonia solanacearum and erwinia carotovora. The preparationprovides new selectivity for the preparation of biocontrol preparations of meloidogyne.

The present invention relates to the determination of the presence of methylmalonic acid in biologic samples including the steps of methylmalonic extraction from the sample; derivatization of methylmalonic acid and use of mass spectrometry with negative mode atmospheric pressure chemical ionization to determine the presence of methymalonic acid through the formation of an ion of mass to charge ratio (m / z) 477. An additional objective of the present invention concerns diagnosis kits for determination of presence and quantification of methylmalonic acid based on the method mentioned before.

The invention provides a method for qualitatively and quantitatively detecting methyl malonic acid in a clinical sample that also may contain succinic acid and homocysteine, said method involving a liquid chromatography separation step followed by a mass spectroscopy detection step, said method comprising the steps of:a) providing a sample that may contain methyl malonic acid and / or succinic acid and optionally homocysteine;b) injecting said sample in a mobile phase comprising a high amount of water-miscible organic solvent;c) eluting said mobile phase containing said sample through a liquid chromatography column containing zwitterionic groups covalently bound to carriers as a stationary phase;d) detecting the possible presence of methyl malonic acid and succinic acid and optionally homocysteine by mass spectroscopy detection; ande) determining the presence and optionally the amounts of said organic molecules using calibration data.

The invention discloses a method for detecting the content of isosuccinic acid in blood or urine. The method comprises the following steps of: respectively uniformly mixing a standard sample and a sample to be detected with an internal standard solution, adding a matrix solution and uniformly mixing; adding an extracting reagent and mixing uniformly and then centrifuging; taking supernatant and blow-drying; adding saturated acidic alcohol solvent, mixing uniformly, sealing and heating to 65 DEG C and derivatizing for 20 min; blow-drying the solvent after the derivatization is completed; adding mobile phase redissolving liquid for redissolving and mixing uniformly; carrying out ultrasonic extraction, mixing uniformly and centrifuging; taking supernatant and enabling the supernatant and the mobile phase to pass through a chromatographic column for liquid-mass spectrum analysis so as to finally detect the content of isosuccinic acid in blood or urine. The method can be used for detecting the content of isosuccinic acid simply, conveniently, quickly and accurately, can be applied to mass sample screening and has the advantages of accurate quantification and strong specificity.

The invention relates to a method and kit for detecting methylmalonic acid in blood plasma through high-performance liquid chromatography-tandem mass spectrometry. The method realizes detection of methylmalonic acid in blood plasma, especially separation of methylmalonic acid in blood plasma from a butanedioic acid detection baseline, through pretreatment and optimized selection of a chromatographic column, chromatographic conditions, and the types and parameters of mass spectrometry. The method is simple and fast, has good precision, accuracy and stability and can provide a more accurate and reliable detection method for clinical practice.

The invention discloses a bisabolane sesquiterpene analogue, and a preparation method and application thereof. The bisabolane sesquiterpene analogue provided by the invention has a structural formula as shown in a formula I. The preparation method for the bisabolane sesquiterpene analogue as shown in the formula I comprises the following steps: 1) subjecting 4-bromo-2-thiophenecarboxaldehyde and p-cyanophenylboric acid to the Suzuki coupling reaction so as to obtain a compound as shown in a formula II; (2) subjecting the compound as shown in the formula II and methylmalonic acid to the Knoevenagel condensation reaction so as to obtain a compound as shown in a formula III; and (3) subjecting ammonia water and a reaction product of the compound as shown in the formula III with thionyl chloride to a reaction an ice bath so as to obtain the compound as shown in the formula I. The compound as shown in the formula I or a pharmaceutically acceptable salt thereof provided by the invention is applicable to preparation of anti-tumor drugs. The anti-tumor drugs can inhibit propagation of tumor cells like breast cancer cells, cervical cancer cells and / or ovarian carcinoma cells; and specifically, the breast cancer cells may be MCF-7 cells, the cervical cancer cells may be HeLa cells, and the ovarian carcinoma cells may be HO8910 cells.

The invention discloses a synthetic method of 2-diester methylmalonate compounds, and relates to the technical field of carboxylic ester preparation. The synthetic method comprises steps as follows: C, sulfonic acid 2-ethyl N-cyanoethanimideate IV and cyanide react under the action of a solvent and a catalyst, and 2-methyl malononitrile V is obtained; D, 2-methyl malononitrile V and ROH react under the action of the solvent and concentrated sulfuric acid, and products of 2-diester methylmalonate compounds I are obtained, wherein MCN is cyanide, M is Na or K, ROH is alkyl alcohol, alkenyl alcohol or a fluoride group containing alcohol, is benzyl alcohol or benzyl alkyl, halogen or nitro substituted benzyl alcohol, or is phenol or C1-C5 containing alkyl, halogen or nitro substituted phenol. The method is unique, the reaction conditions are mild, the reaction process is basically free of by-products, the yield is high, adopted raw materials have extensive sources, and acetaldehyde can be used as the raw material; the synthetic method is applicable to industrial production.

The invention provides a preparation method of a 4-methoxyl pyrrole intermediate. The preparation method comprises the following steps: (1) carrying out a reaction on a compound 2,4-difluorobenzaldehyde represented as in a formula (I) with a compound p-toluenesulfonic acid represented in a formula (II) under a condition of an alkaline solvent and acid catalysis to obtain a compound represented asin a formula (III); (2) carrying out a reaction on the compound represented as in the formula (III) with a compound phosphorus oxychloride in the presence of an alkali to obtain a compound representedas in a formula (IV); and (3) carrying out a reaction on the compound represented as in the formula (IV) with a compound 2-(methoxymethylene)dimethyl malonate represented as in a formula (V) to obtain a compound (4-methoxyl pyrrole intermediate) represented as in a formula (AB03288). According to the method, the raw materials for preparing the 4-methoxy pyrrole intermediate are easy to obtain, the reaction conditions of all the steps are mild, purification is easy, operation is simple, and the yield is high.

The invention discloses a urine treatment method. The urine treatment method comprises the following steps that a certain number of urine samples are taken, an interior label and frozen absolute ethyl alcohol are added, the mixture is fully oscillated and mixed uniformly, centrifugal treatment is carried out for removing protein, supernatant liquor is taken, nitrogen blowing is carried out until the supernatant liquor is fully dried, and derivatization reagent is added for reaction. The invention further discloses a method for detecting urine methylmalonic acid through gas chromatography and mass spectrometry. The method includes the urine treatment method and optimizes the instrument condition of gas chromatography and mass spectrometry detection after the treatment. By the adoption of the urine treatment and detection method, time for urine sample treatment is greatly saved, and organic acid, especially methylmalonic acid can be detected easily, fast and accurately.

The invention provides polyester resin for the self-leveling powder coating and a preparation method. Pentaerythritol, adipic acid, glycerol, terephthalic acid, isocaprylic acid, n-butyric acid, diethylene glycol diglycidyl ether and methylmalonic acid are used as main raw materials; the polyester resin for the disposable low-temperature curable self-leveling powder coating is obtained by adopting a segmented polymerization mode. The polyester structure has a proper amount of ester groups with low surface tension, so that the softening point of the product is reduced, low-temperature curing is realized, the problem of insufficient leveling property of a low-temperature curing coating film is solved due to excellent self-leveling property, and the ultrahigh-leveling coating film is obtained. According to the preparation process, special raw materials do not need to be prepared in advance, the 70 / 30 self-leveling polyester resin product is directly prepared by adopting a single-kettle synthesis process, the process is simple, and the cost is relatively low.

The invention discloses a process method for preparing sodium 2-ketobutyrate. The method comprises the following steps: S1, reacting diethyl methylmalonate or dimethyl methylmalonate used as a raw material with diethyl oxalate or dimethyl oxalate under the catalysis of a proper amount of an alkaline compound to obtain a 1-carbonyl propane-1,2,2-tricarboxylate intermediate; S2, reacting the 1-carbonyl propane-1,2,2-tricarboxylate intermediate obtained in step S1 with water under the catalysis of a proper amount of an acidic compound to obtain 2-ketobutyric acid; and S3, reacting the 2-ketobutyric acid obtained in step S2 with the alkaline compound to obtain the sodium 2-ketobutyrate. The method is reasonable in route design, safe, reliable, low in cost and capable of achieving quantitativeproduction.

The invention relates to a preparation method of 4, 7-dichloroquinoline in the technical field of medicines, and the method comprises the following steps of: carrying out condensation reaction on 2-amino-6-chlorobenzoic acid (I) serving as an initial raw material and diethyl ethoxymethylenemalonate (II) in an organic solvent to obtain (III), and carrying out cyclizing, hydrolyzing and acidifying on (III) to obtain hydroxyquinoline dicarboxylic acid (V). performing high-temperature decarboxylation on V to obtain hydroxyquinoline (VI), and chlorinating VI with phosphorus oxychloride to obtain 4,7-dichloroquinoline. The 4, 7-dichloroquinoline prepared by the method disclosed by the invention does not contain isomer 4, 5-dichloroquinoline, and the temperature of the condensation reaction is low under the catalysis of the catalyst, so that the reaction yield is improved, the generation of impurities is avoided, the energy consumption is reduced, and the economic benefit of the product is improved.

The invention provides a method for detecting the content of methylmalonic acid in blood, wherein the comprises the following steps: under certain detection conditions, respectively detecting at least three standard solutions by using a high performance liquid tandem mass spectrometer to obtain chromatograms of the standard solutions, wherein each standard solution contains a methylmalonic acid standard substance with known concentration and an internal standard substance; fitting according to the chromatograms of the standard solutions to obtain a standard curve equation; uniformly mixing the internal standard working solution and a blood sample, adding acetonitrile, uniformly mixing, centrifuging to obtain a supernatant, blow-drying the supernatant in a heating and nitrogen blowing manner, adding an aqueous solution containing 0-2% of formic acid and 0-20% of acetonitrile after blow-drying, uniformly mixing, and centrifuging to obtain a supernatant to obtain a to-be-detected sample; detecting the to-be-detected sample to obtain a chromatogram of the to-be-detected sample; and calculating the content of methylmalonic acid in the blood sample according to the chromatogram of the to-be-detected sample and the standard curve equation. According to the scheme, the method for detecting the content of methylmalonic acid in blood is provided, the sample pretreatment operation is simple, and the cost is low.

The invention provides a non-woven fabric and a preparation system and a preparation process thereof. The non-woven fabric comprises, by weight, 77 parts of acrylamide, 54 parts of maleic anhydride, 3parts of 5-hydroxymethyl furfural, 0.6 part of methylmalonic acid, 2 parts of 2,2'-azobis[2-methylpropionamidine] dihydrochlorid, 4 parts of ammonium bicarbonate and 500 parts of water. The preparation process comprises the steps of 1, guiding a raw material solution into a melt-blowing die head part through a discharge assembly; 2, starting a coagulation prevention mechanism to drive a stock solution to be sprayed out and stir the solution simultaneously; 3, starting two speed adjusting mechanisms to drive a spraying pressing plate to move downwards to press out the solution in the melt-blowing die head part, and spraying the solution onto a receiving device to form the non-woven fabric under the action of a high-temperature airflow generator and a cooling airflow generator; and 4, collecting the non-woven fabric onto a collection roller.

The invention relates to the detection of methylmalonic acid (MMA). In a particular aspect, the invention relates to methods for detecting derivatized methylmalonic acid (MMA) by mass spectrometry.

The invention discloses high-strength impact-resistant melamine resin tableware and a preparation method thereof. The high-strength impact-resistant melamine resin tableware is prepared from the following raw materials in parts by weight: 60 to 75 parts of melamine-formaldehyde resin, 16 to 22 parts of ethylene-methyl methacrylate copolymer powder, 25 to 33 parts of an elastomer, 5 to 8 parts of cellulose, 1.5 to 2.5 parts of zinc peroxide, 3 to 5 parts of aluminum borate whiskers, 3 to 5 parts of nano silicon dioxide, 2.5 to 3.5 parts of zinc stearate, 1 to 2 parts of triethanolamine and 1.5 to 2 parts of methylmalonic acid. The high-strength impact-resistant melamine resin tableware is high in impact strength, high in tensile strength, excellent in mechanical property, resistant to impact (impact), not prone to damage and cracking and the like; the migration volume of free formaldehyde is low, and the maximum migration volume of lead and cadmium is also low and is lower than the migration volume of free formaldehyde and the maximum migration volume of lead and cadmium in a comparison file 1, so that the tableware is healthy, environment-friendly and non-toxic; and in addition, the thermal decomposition initial temperature reaches 145 DEG C or above, the thermal decomposition initial temperature is high, and the heat resistance is good.

The invention discloses a method and a kit for rapidly detecting the content of methylmalonic acid, methyl citric acid, methionine and total homocysteine in dried blood spots. A non-derivatization method is adopted, a UPLC-MS / MS technology is adopted to carry out internal standard method quantitative analysis on a sample, six levels of blood spots are used as correction lines, the concentration ratio of a standard substance solution to an internal standard substance is used as an X axis, and the peak area ratio of the standard substance to the internal standard substance is used as a Y axis; and performing linear regression analysis to obtain a regression equation, substituting the corresponding area of the to-be-detected substance into the regression equation, and calculating the concentration of the to-be-detected substance in the sample. According to the method, methylmalonic acid (MMA), methyl citric acid (MCA), total homocysteine (tHCY) and methionine (MET) can be detected at the same time, the sample size is three blood spots with the diameter of 3.2 mm, sample pretreatment is simple, the specificity is high, time consumed for a single sample is 3 minutes, and high-throughput detection can be achieved.

The invention relates to a preparation method of diethyl methylmalonate by taking heteropolyacid as a catalyst. The method comprises the following steps: adding 2-cyanopropionic acid into ethanol fordissolving, controlling the molar ratio of 2-cyanopropionic acid to ethanol to be 1:(3-4), and transferring the obtained solution into a reaction kettle after dissolving; stirring at room temperature,adding the heteropolyacid catalyst, controlling the mass ratio of 2-cyanopropionic acid to the heteropolyacid catalyst to be 2:(1-2), controlling the temperature to be 65-80 DEG C, reacting for 3-4 h, and then ending the reaction; distilling to separate ethanol, adding ammonia water to neutralize, regulating the pH value to be neutral, and fractionating to obtain crude diethyl methylmalonate; andputting the crude diethyl methylmalonate into a rectifying tower, and carrying out reduced pressure rectification to obtain diethyl methylmalonate. Sulfuric acid is replaced with the heteropolyacid catalyst for an esterification reaction, so the method has the advantages of good selectivity, high catalytic activity, high regeneration speed and small corrosion to equipment.

The present disclosure teaches systems and methods of diagnosing and treating the distinct biologic components that contribute to chronic pain symptoms experienced by patients. A biologic sample is obtained from a patient. Levels of two or more biomarkers (e.g., methylmalonic acid, homocysteine, xanthurenic acid, 3-hydroxypropyl mercapturic acid (3-HPMA), pyroglutamate, hydroxymethylglutarate (HMG), quinolinic acid, kynurenine acid, 5-hydroxyindoleacetate (5-HIAA), vanilmandelate (VMA), and ethylmalonic acid) in the biologic sample are experimentally determined. Based on the existence of abnormal results in one or more biomarkers the patient is diagnosed as having the nerve health, oxidative stress, chronic inflammation pain, and / or neurotransmitter pain components to their chronic pain. Based on the resulting diagnoses administration of certain support compounds is directed. The patient may retest after a sufficient period of time to observe any longitudinal differences in the test results and adjust treatment accordingly. Further, the biomarker data gathered from pain-neutral and chronic pain patients (particularly those using opioid therapies) will be used to characterize biochemistries going forward.

The invention relates to a quality control product for urine organic acid filter paper and a preparation method thereof. The preparation method comprises the following steps: (1) preparing an organic acid standard liquid: separately preparing standard solutions of methylmalonic acid, glutaric acid, isoamyl glycine, phenylpyruvic acid, orotic acid and 4-hydroyl phenyllactic acid; (2) preparing a working liquid: by taking urine as a solvent, adding the organic acid standard liquid to obtain the working liquid; and (3) dropwise adding the working liquid onto the filter paper, and airing the filter paper to obtain the quality control product for the urine organic acid filter paper. The quality control product for the urine organic acid filter paper prepared by the preparation method provided by the invention can be stored for at least two months under a condition of 20 DEG C below. The uniformity and stability of the urine organic acid filter paper taken out to restore the constant temperature under the condition of 20 DEG C below can meet the requirements on the quality control product.

By determining the lethality rate to Meloidogyne incognita and Caenorhabditis elegans, it was found that the methylmalonic acid has a better nematicidal effect on the Caenorhabditis elegans, with the LC50 being 13.11 and 1.20 for the Meloidogyne incognita and the Caenorhabditis elegans, respectively. After compounding the methylmalonic acid with betaine, the LC50 was 2.85 and 0.27 for the Meloidogyne incognita and the Caenorhabditis elegans, respectively. Meanwhile, the methylmalonic acid also has an inhibiting effect on Pseudomonas solanacearum and Erwinia carotovora. The preparation of the methylmalonic acid provides a new choice for preparing novel biocontrol agents against the root-knot nematodes.

The invention discloses a preparation method of ethyoxyl methylene diethyl malonate, and the method comprises the following steps: taking diethyl malonate as a raw material and ethyl formate or carbon monoxide as an auxiliary material, introducing formyl under the action of a catalyst, and carrying out condensation reaction with alcohol under the catalysis of acid to obtain the product ethyoxyl methylene diethyl malonate, wherein ethyl formate can be replaced by CO, and the raw material cost is lower. The method has the advantages of simple operation, easily available raw materials, high conversion rate, safety, environmental protection and low cost, and can realize industrial production.

The invention relates to a preparation method of 4,7-dichloroquinoline in the technical field of medicine, which uses 2-amino-6-chlorobenzoic acid (I) as a starting material, and ethoxymethine in an organic solvent Diethyl malonate (II) undergoes condensation reaction to obtain (Ⅲ), III undergoes cyclization, hydrolysis, and acidification to obtain hydroxyquinoline dicarboxylic acid (V), V undergoes high-temperature decarboxylation to obtain hydroxyquinoline (VI), and VI undergoes Chlorination of phosphorus oxychloride gives 4,7-dichloroquinoline. The 4,7-dichloroquinoline prepared by the method of the present invention does not have the isomer 4,5-dichloroquinoline, and the temperature of the condensation reaction under the catalysis of the catalyst is low, which improves the yield of the reaction and avoids the generation of impurities , and reduce energy consumption, improve the economic benefits of the product.

The invention discloses a preparation method of an alminoprofen intermediate, belongs to the field of organic chemical synthesis, and provides a new technical route for improving the defects of synthetic reaction of ethyl 2-(4-amino-phenyl)propionate: 1) taking p-fluoronitrobenzene and diethyl methylmalonate as raw materials, adding alkali, conducting heating, finishing the reaction, conducting cooling, conducting hydrolyzing with alkali, after the reaction is finished, conducting layering, conducting washing with water, extracting impurities, adjusting the pH value, extracting a product, and conducting concentrating to obtain AMLF02; 2) heating AMLF02 in a solvent to 55-75 DEG C, and dropwise adding thionyl chloride for esterification reaction, conducting cooling, adjusting the pH, directly adding a catalyst for reduction at the temperature of 70-80 DEG C, after the reaction is finished, conducting filtering with the aid of diatomite, neutralizing the filtrate to 7-8, conducting concentrating to remove ethanol, extracting a water phase with methylbenzene, and conducting concentrating to obtain AMLF04. The method has the beneficial effects that the problem of slow filtration is solved, extraction is omitted, time is saved, and cost is saved; direct layering is performed after reaction, the amount of hydrochloric acid is reduced, and the treatment difficulty of three wastes is reduced; and safe hydrazine hydrate replaces active nickel.

The invention relates to a flame retardant for computer display, and belongs to the technical field of flame retardant articles. The flame retardant is prepared the following raw materials: water, N,N-dimethylaminoethyl acrylate, dimethyl methylmalonate, 2,3-dimethyl-2,3-butanediol, 2,2-dimethyl-1,3-propilidene dimethacrylate, phenylacrylic acid, and 1-(5-bicyclo [2.2.1] heptyl-2-vinyl)-1-phenyl-3-(1-piperidyl) propyl-1-alcohol hydrochloride. The flame retardant for computer display uses the interaction and mutual influence of correlative chemical components to effectively realize flame retardant effect. The flame retardant has stable chemical properties, does not decompose under high temperature, is attached to the surface of the plastic display, has no effect on the computer display, and can effectively prevent the spreading of flame, prevent the formation of computer fire, thus the flame retardant has excellent flame retardant effect.

The invention discloses an application of methylmalonic acid as a biomarker in predicting heart failure after myocardial infarction. The invention also discloses application of the reagent or the instrument for detecting methylmalonic acid in preparation of the reagent or the device for predicting heart failure after myocardial infarction. Wherein preferably, the device is a rapid separation liquid chromatogram-quadrupole rod-flight time mass spectrum combined instrument. According to the invention, MMA is found as a biological prediction target for predicting heart failure after myocardial infarction for the first time, and the blank in the field that a current conventional biomarker cannot accurately predict heart failure after myocardial infarction is filled up. According to the application disclosed by the invention, the treatment strategy of patients with myocardial infarction is greatly improved, a new technical means is provided for the occurrence mechanism and early diagnosis of heart failure after myocardial infarction, and a potential theoretical basis is also provided for effective treatment of heart failure after myocardial infarction.

The invention discloses a preparation method of topramezone. The particularly comprises the steps of carrying out cyclization, methylation, hydrolysis, amidation, Grignard reaction, oxidation and demethylation on 2-(alkoxymethylene)malonate as a raw material, so as to obtain a target product, wherein the yield of the target product reaches 61.8%-63.2%. According to the preparation method, the raw materials such as a palladium / platinum catalyst which is high in cost and difficult to recycle, a virulent rearrangement catalyst and high-cost methylhydrazine are not adopted, and the preparation method which is low in cost, easy in operation, low in pollution and accordant with large-scale industrial production requirements is provided.

The invention provides a magnesium binding agent and a method for preparing a refractory material by using the magnesium binding agent. Magnesium oxide, magnesium oleate and methylmalonic acid are used as binding agent components. 0.10-0.15% of magnesium oleate and methylmalonic acid are added, and the ratio of magnesium oleate to methylmalonic acid is set to be 1 / 1 to 2 / 1, so that the microstructure of magnesium hydroxide generated in hydration reaction of magnesium oxide is controlled. Meanwhile, through the implementation process of preparing the refractory material by using the magnesian binding agent, the magnesian binding agent forms an embedded structure in the refractory material, so that the strength of a binding phase of the magnesian binding agent and the refractory material isgreatly improved, the microstructure of a magnesium binding agent added sample is conical, and the compressive strength of the magnesium binding agent added sample can reach 60-70 MPa. The magnesium binding agent and the using method thereof are simple, and the preparation cost is low.