14 results about "Phenylpyruvic acid" patented technology

The invention discloses a recombinant microorganism for producing hydroxytyrosol as well as a construction method and application of the recombinant microorganism. According to the recombinant microorganism, 3-deoxy-D-arabinoheptulose-7-phosphate synthetase aroGfbr, chorismate mutase / pre-benzoic acid dehydrogenase tyrAfbr, 4-hydroxyphenylacetic acid 3-hydroxylase HpaBC, phenylpyruvate decarboxylase ARO10 and alcohol dehydrogenase ADH6 are subjected to overexpression, and the recombinant microorganism can be used for preparing the recombinant microorganism. The genome of the gene naturally comprises a citrate synthase coding gene gltA and a tyrosine aminotransferase coding gene tyrB; the original promoter of the gltA and / or the original promoter of the tyrB are / is replaced by a time sequence promoter PrrnC-37. When the recombinant microorganism is used for producing hydroxytyrosol, the production efficiency can be improved, and the production cost of hydroxytyrosol can be reduced.

Various methods and compositions of treating 4-hydroxyphenylpyruvate dioxygenase-like (HPDL)-related diseases or disorders are presented herein. Also presented herein are methods of increasing CoQ10 biosynthesis, and methods of determining whether a subject will benefit from a CoQ10 or CoQ10 alternative treatment. Also presented herein are pharmaceutical compositions and dosage forms comprising 4-hydroxymandelic acid (4-HMA), and / or its metabolites. Further presented herein are compounds that inhibit 4-hydroxyphenylpyruvate dioxygenase-like (HPDL). Further presented herein are methods of identifying and / or assessing modulators of HPDL. Yet further presented herein are example methods and systems for isotopic labelling in cells by metabolizing cells in the presence of gaseous isotopic tracer.

The invention provides a phenylpyruvate decarboxylase mutant with multi-site mutation and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The problem of low yield of tyrosol is solved. The phenylpyruvate decarboxylase mutant comprises a phenylpyruvate decarboxylase mutant, and the phenylpyruvate decarboxylase mutant is obtained by mutating any two or three sites of the 93rd site, the 106th site, the 153rd site, the 220th site, the 242nd site, the 273rd site and the 285th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The method is mainly used for increasing the yield of tyrosol.

This invention discloses a recombinant *E. coli* strain for producing phenyllactic acid, a lactate dehydrogenase mutant, a whole-cell catalytic method for preparing phenyllactic acid, and the application of the aforementioned recombinant *E. coli* strain or lactate dehydrogenase mutant in the catalytic preparation of phenylpyruvic acid from phenyllactic acid. This invention significantly improves catalytic efficiency by modifying the 52nd amino acid of lactate dehydrogenase through site-directed mutagenesis. After mutating glutamine (Q) to valine (V) at position 52 of the *Lactobacillus mucosa* lactate dehydrogenase, the catalytic efficiency is significantly improved even at low cell density (OD). 600 =15) and substrate 15 g / L conditions, almost complete conversion was achieved; and combined with high-density catalysis and surface display optimization, the yield of phenyl lactic acid was increased while the downstream separation and purification costs were significantly reduced, greatly enhancing the potential for industrial production.

Provided herein are compositions and methods for increasing the amount aryl-lactates in fermented foods. The methods can comprise delivering alpha-ketoglutarate (AKG), trisodium citrate dehydrate (CIT), or both AKG and CIT; and / or phenylpyruvic acid (PPyA), 4-hydroxyphenylpyruvic acid (4HPPyA), indole-3-pyruvic acid (IPyA) or combinations thereof; and / or phenylalanine (Phe), tyrosine (Tyr), and / or tryptophan or combinations thereof to fermented foods and fermented food matrices. Optionally, bacteria with aromatic amino transferase (ArAT) and / or phenyllactate dehydrogenase activity can also be added to the fermented foods and fermented food matrices. Also provided are methods to increase the bioactivity of fermented foods towards an immune receptor (aryl hydrocarbon receptor—AhR). Also provided are methods to more effectively maintain food matrix AhR bioactivity across food storage times of up to 4 weeks. Also provided are methods of inducing weight loss, reducing fat mass, and / or reducing glucose intolerance in mammals.

The application discloses a kind of functionalized graphene materials, preparation method and its application in phenylpyruvic acid preparation, belong to graphene catalytic organic drug synthesis technical field, 4-dimethylaminopyridine is added to the prepared graphene solution, ultrasonic treatment is then carried out under the condition of 120~180 DEG C for 12~36h, the product is filtered, washed and dried after reaction is finished and naturally cooled to room temperature, and black solid is obtained as functionalized graphene material DMAP-rGO, which is used as catalyst to prepare phenylpyruvic acid, has better stability and reusability, and can be widely used in industrial production.

The application discloses a genetically engineered bacterium with high yield of gallic acid and application thereof, and belongs to the technical field of gene recombination.The genetically engineered bacterium with high yield of gallic acid is prepared from Yarrowia lipolytica ATCC MYA-2613 as a starting strain, and the ku70 gene is knocked out; the gene coding 4-hydroxyphenylpyruvate dioxygenase is expressed; the shikimic acid pathway is strengthened; and three copies of tHMGR and the lysine defect are integrated.In 2L fermenter fed-batch fermentation, the yield of gallic acid is 33.71g / L, which is more than 10 times higher than the highest yield of gallic acid of 2.8g / L of the genetically engineered Yarrowia lipolytica reported at present in 2L fermenter fed-batch fermentation, and the application lays a solid foundation for industrialization of Yarrowia lipolytica for producing gallic acid, and has an industrial application prospect.

A herbicidal composition and a method for controlling weeds, including one or more uracil compounds of formula (I) and a compound of formula (II), and a herbicide compound of group B and a safener of group C. A weight ratio of the uracil compounds to the herbicide compound group B and the safener group C is 1:0.02 to 1:50, and the herbicide compound of group B is one or more of compounds B-1 to B-12: B-1, acetolactate synthase inhibitors; B-2, acetyl CoA carboxylase inhibitors; B-3, protoporphyrinogen IX oxidase inhibitors; B-4, 4-hydrophenylpyrubic acid dioxygenase inhibitors; B-5, phytoene desaturase inhibitors; B-6, photosystem II inhibitors; B-7, very-long-chain fatty acid synthesis inhibitors; B-8, microtubule formation inhibitors; B-9, auxin-type herbicides; B-10, enolpyruvylshikimate-3-phosphate synthase inhibitors; B-11, glutamine synthase inhibitors; and B-12, other herbicides; or agriculturally acceptable salts or derivatives thereof

This invention relates to a phenylpyruvate decarboxylase mutant for the synthesis of phenylethanol and its applications, belonging to the fields of enzyme engineering and genetic engineering. To address the technical problems of low catalytic efficiency, poor substrate affinity, and insufficient stability of existing phenylpyruvate decarboxylases, which lead to low biosynthetic efficiency and limited yield of phenylethanol, making it difficult to meet the needs of industrial production, this invention provides a phenylpyruvate decarboxylase mutant for the synthesis of phenylethanol. This mutant offers a new and highly efficient synthetic element for the biosynthesis of phenylethanol, with broad application prospects and market potential in the field of higher alcohol synthesis.

The invention discloses a phenylpyruvate decarboxylase mutant and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The purpose of the present invention is to increase the yield of tyrosol. The phenylpyruvate decarboxylase mutant provided by the invention is obtained by mutating any one of the 56th site, the 92th site, the 93th site, the 106th site, the 115th site, the 153th site, the 178th site, the 188th site, the 214th site, the 220th site, the 234th site, the 242th site, the 273th site, the 285th site, the 371th site, the 416th site, the 581th site and the 621th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The phenylpyruvate decarboxylase is mainly used for high-efficiency phenylpyruvate decarboxylase and application thereof in de novo synthesis of tyrosol.

The present application relates to a kind of engineering strains of escherichia coli for producing β, γ-type diol and its construction method and application, which method is by constructing the recombinant plasmid pET-ARO10 of gene ARO10 expressing phenylpyruvic acid decarboxylase;Construction of the recombinant plasmid pRSF-ScIlv2c of gene ScIlv2 expressing acetyl-hydroxy acid synthase;The recombinant plasmid pET-ARO10 and the recombinant plasmid pRSF-ScIlv2c are introduced into escherichia coli, to obtain the engineering strains of escherichia coli for producing β, γ-type diol.The engineering strains of escherichia coli can produce β, γ-type diol 4-methyl pentane-2, 3-diol, 5-methyl hexane-2, 3-diol, 4-methyl hexane-2, 3-diol, realize the efficient synthesis of β, γ-type diol in escherichia coli, can be applied to medicine, biofuel, novel polymer material and the like industry.

The invention discloses escherichia coli recombinant bacteria capable of producing hydroxytyrosol at high yield as well as a construction method and application of the escherichia coli recombinant bacteria. The invention provides a method for constructing Escherichia coli with high yield of hydroxytyrosol. A novel hydroxylase combination obtained through verification can be used for producing hydroxytyrosol, and a key function of phenylpyruvate decarboxylase in a conversion process from 4-hydroxyphenylpyruvic acid to hydroxytyrosol is determined; the Escherichia coli strain constructed by the invention can accumulate 9.2 g / L of hydroxytyrosol within 48 hours, lays a foundation for low-cost and high-efficiency industrial production of hydroxytyrosol, and has a wide application prospect.

The invention belongs to the field of functional food and natural product chemistry, and particularly relates to a polygonatum sibiricum tea composition with a lipid regulating function and a preparation method of the polygonatum sibiricum tea composition. The composition is prepared from a modified rhizoma polygonati extract, 4-hydroxy-3-methoxyphenylpyruvic acid, green tea powder, lotus leaf powder, malt flour and a fructus momordicae extract. The modified polygonatum sibiricum extract is prepared by modifying a polygonatum sibiricum extracting solution through a gallic acid-L-serine deep eutectic system, an esterification and hydrogen bond recombination structure is formed in the modification process, and the hydrophobicity, the oxidation resistance and the lipid binding capacity of polygonatum sibiricum polysaccharide are remarkably improved. 4-hydroxy-3-methoxyphenylpyruvic acid is used as natural aromatic organic acid, and has a synergistic effect with the modified rhizoma polygonati extract, so that cholesterol excretion can be promoted, and lipid peroxidation can be inhibited. The composition disclosed by the invention has excellent performance in the aspects of reducing serum cholesterol and triglyceride, improving the free radical scavenging rate and maintaining the stability of the tea powder, is obviously superior to an unmodified or single modified system, and has structural innovation and a good application prospect.

The invention provides a method for biosynthesizing benzofuranone and 5-hydroxybenzofuranone. Firstly, Car is modified, CarAT is obtained and combined with phosphopantetheine enzyme sfp to construct plasmids, wild escherichia coli BW25113 serves as host bacteria, 2-hydroxyphenylacetic acid serves as a substrate, 2, 5-dihydroxyphenylacetic acid serves as a substrate, an addition experiment is conducted, and it is determined that the modified enzyme can catalyze conversion of 2-hydroxyphenylacetic acid to generate benzofuranone and can catalyze conversion of 2, 5-dihydroxyphenylacetic acid to generate 2, 5-dihydroxyphenylacetic acid. The method comprises the following steps: converting 2, 5-dihydroxyphenylacetic acid into 5-hydroxybenzofuranone, then respectively performing overexpression on corresponding genes to increase precursor flux, and constructing related plasmids in combination with 4-hydroxyphenylpyruvate dioxygenase HPPD (4-hydroxyphenylpyruvate dioxygenase) derived from streptomyces coelicolor; wherein the HPPD can be used for converting phenylpyruvic acid into 2-hydroxyphenylacetic acid and converting 4-hydroxyphenylpyruvic acid into 2, 5-dihydroxyphenylacetic acid under the assistance of Fe < 2 + >, PZE-HPPD-PheAfbr-aroGfbr and PZE-HPPD-TyrAfbr-aroGfbr are respectively combined with PCS-CarAT-sfp, then a de novo synthesis experiment is carried out by using wild escherichia coli BW25113, and the de novo synthesis of benzofuranone and 5-hydroxybenzofuranone is realized.