2 results about "Phenylpyruvic acid" patented technology

This invention discloses a recombinant *E. coli* strain for producing phenyllactic acid, a lactate dehydrogenase mutant, a whole-cell catalytic method for preparing phenyllactic acid, and the application of the aforementioned recombinant *E. coli* strain or lactate dehydrogenase mutant in the catalytic preparation of phenylpyruvic acid from phenyllactic acid. This invention significantly improves catalytic efficiency by modifying the 52nd amino acid of lactate dehydrogenase through site-directed mutagenesis. After mutating glutamine (Q) to valine (V) at position 52 of the *Lactobacillus mucosa* lactate dehydrogenase, the catalytic efficiency is significantly improved even at low cell density (OD). 600 =15) and substrate 15 g / L conditions, almost complete conversion was achieved; and combined with high-density catalysis and surface display optimization, the yield of phenyl lactic acid was increased while the downstream separation and purification costs were significantly reduced, greatly enhancing the potential for industrial production.

This invention relates to a phenylpyruvate decarboxylase mutant for the synthesis of phenylethanol and its applications, belonging to the fields of enzyme engineering and genetic engineering. To address the technical problems of low catalytic efficiency, poor substrate affinity, and insufficient stability of existing phenylpyruvate decarboxylases, which lead to low biosynthetic efficiency and limited yield of phenylethanol, making it difficult to meet the needs of industrial production, this invention provides a phenylpyruvate decarboxylase mutant for the synthesis of phenylethanol. This mutant offers a new and highly efficient synthetic element for the biosynthesis of phenylethanol, with broad application prospects and market potential in the field of higher alcohol synthesis.