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Lactobacillus buchneri 8-2N and application thereof in preparation of phenyllactic acid

A technology of Lactobacillus Brucella and phenyllactic acid, which is applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low phenyllactic acid conversion yield, low concentration, and difficulty in meeting industrial production requirements, and achieve High conversion rate, good biological safety, good synthetic conversion and phenyllactic acid production effect

Active Publication Date: 2017-02-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some existing strains have potential biosafety problems under certain conditions, and some strains show obvious inhibition when the substrate or product concentration is low, so that the conversion yield of phenyllactic acid is low and the concentration is low, making it difficult to Meet the requirements of industrial production

Method used

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  • Lactobacillus buchneri 8-2N and application thereof in preparation of phenyllactic acid
  • Lactobacillus buchneri 8-2N and application thereof in preparation of phenyllactic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Strain screening

[0026] The present invention isolates and screens bacterial strains from traditional Chinese pickles, dilutes a certain amount of pickles juice in sterile water, and dilutes them in a solid medium containing calcium carbonate (composition: 20 g of glucose, 10 g of peptone, 2 g of diamine hydrogen citrate, anhydrous acetic acid) Sodium 5g, manganese sulfate 0.25g, magnesium sulfate 0.58g, dipotassium hydrogen phosphate 2g, yeast extract 5g, beef extract 10g, Tween 85g, agar 23g, calcium carbonate 34g, deionized water 1L, pH value natural) on the initial Sieve, temperature 30°C, static culture, time 24-72h; select a single colony negative for hydrogen peroxide, inoculate in liquid medium (composition: glucose 20g, peptone 10g, diamine hydrogen citrate 2g, anhydrous sodium acetate 5g , 0.25g of manganese sulfate, 0.58g of magnesium sulfate, 2g of dipotassium hydrogen phosphate, 5g of yeast extract, 10g of beef extract, 85g of Tween, 23g of agar, 1L of...

Embodiment 2

[0032] (1) Slant culture: inoculate Lactobacillus buchneri 8-2N into the slant medium, and culture at 25°C for 2 days to obtain slant bacteria; the final concentration of the slant medium consists of: glucose 20g, peptone 10g, hydrogen citrate Diamine 2g, anhydrous sodium acetate 5g, manganese sulfate 0.25g, magnesium sulfate 0.58g, dipotassium hydrogen phosphate 2g, yeast extract 5g, beef extract 10g, Tween 85g, agar 23g, deionized water 1L, pH value is natural.

[0033] (2) Seed culture: inoculate the slant bacteria into the seed medium, cultivate at 20°C for 24h, and obtain the seed liquid; the final concentration of the seed medium consists of: glucose 20g, peptone 10g, diamine hydrogen citrate 2g, no Water sodium acetate 5g, manganese sulfate 0.25g, magnesium sulfate 0.58g, dipotassium hydrogen phosphate 2g, yeast extract 5g, beef extract 10g, Tween 85g, deionized water 1L, pH value is natural.

[0034] (3) Fermentation culture: inoculate about 1L of fermentation medium w...

Embodiment 3

[0036] (1) Slant culture: inoculate Lactobacillus buchneri 8-2N into the slant medium, culture at 35°C for 1 day to obtain the slant bacteria; the final concentration of the slant medium consists of: glucose 20g, peptone 10g, hydrogen citrate Diamine 2g, anhydrous sodium acetate 5g, manganese sulfate 0.25g, magnesium sulfate 0.58g, dipotassium hydrogen phosphate 2g, yeast extract 5g, beef extract 10g, Tween 85g, agar 23g, deionized water 1L, pH value is natural.

[0037] (2) Seed culture: inoculate the slant bacteria into the seed medium, cultivate at 35°C for 12h, and obtain the seed liquid; the final concentration of the seed medium consists of: glucose 20g, peptone 10g, diamine hydrogen citrate 2g, no Water sodium acetate 5g, manganese sulfate 0.25g, magnesium sulfate 0.58g, dipotassium hydrogen phosphate 2g, yeast extract 5g, beef extract 10g, Tween 85g, deionized water 1L, pH value is natural.

[0038] (3) Fermentation culture: inoculate about 0.5L of fermentation medium ...

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Abstract

The invention discloses lactobacillus buchneri 8-2N and application thereof in preparation of phenyllactic acid. A strain provided by the invention is used as an original strain for synthesizing phenyllactic acid through metabolism by virtue of a microbial fermentation method; and a bacterial cell of the fermented strain is taken as a whole-cell catalyst, phenylpyruvic acid is taken as a key substrate, and synthesized phenyllactic acid can be converted by virtue of a microbial conversion method. The strain is separated from traditional pickles, has good biosecurity and very good capacity of producing phenyllactic acid through synthesis conversion, can grow relatively rapidly in a conventional MRS fermentation culture solution and has a moderate fermentation cycle; when the bacterial cell is used for converting synthesized phenyllactic acid, the conversion reaction time is short, the conversion rate is high, the concentration of phenyllactic acid in a conversion solution can reach above 9g / L, and the conversion rate can reach 80%-90%; and lactobacillus buchneri 8-2N has a relatively good application prospect.

Description

[0001] (1) Technical field [0002] The invention relates to the preparation of phenyllactic acid, in particular to a new bacterial strain-Lactobacillus brauchneri 8-2N and its application in the preparation of phenyllactic acid. [0003] (2) Background technology [0004] The use of microbial fermentation or transformation to synthesize chemicals or biochemicals usually has the advantages of mild preparation conditions, environmental friendliness, and relatively simple synthesis routes. It is an important development direction in the chemical and biological fields in recent years. [0005] Phenyllactic acid is a new type of antibacterial agent with broad-spectrum antibacterial properties, which can be used for the preservation of food and medicine; phenyllactic acid is also an important monomer for the synthesis of polymer materials, and is also a key precursor for the synthesis of anti-platelet aggregation and myocardial infarction drugs Therefore, it has important applicatio...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/42C12R1/225
CPCC12P7/42C12N1/205C12R2001/225
Inventor 贠军贤倪正沈绍传关今韬徐林红郑巧东姚克俭关怡新姚善泾
Owner ZHEJIANG UNIV OF TECH
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