40 results about "Lactobacillus buchneri" patented technology

The invention belongs to the technical field of probiotics and application thereof, and relates to a probiotic capable of reducing uric acid and a product thereof, in particular to a strain of Lentilactobacillusbuchneri strain ZD62 which is preserved in China Center for Type Culture Collection on March 14, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2024469, and the preservation number is CCTCC NO: M2024469, the preservation number is CCTCC NO: M2024469, the preservation number is CCTCC NO: M2024469, and the preservation number is CCTCC NO: M2024469. The invention relates to a compound probiotic agent, which is prepared from Lentilactobacilli buchneri strain ZD62 and Mycobacterium fermentum ZD103, and is characterized in that the Mycobacterium fermentum ZD103 is prepared from Lentilactobacilli buchneri strain ZD62; the lactobacillus buchneri ZD62 and the mucus fermentum ZD103 provided by the invention have a good characteristic of reducing uric acid.

The invention discloses a lactobacillus buchneri complex microbial inoculant and application thereof, and belongs to the technical field of microorganisms. In order to solve the problem of poor direct degradation effect of lactic acid bacteria on uric acid in the prior art, the invention provides a complex microbial inoculant containing lactobacillus buchneri NEFU-1 and NEFU-2, and the complex microbial inoculant has the functions of degrading uric acid, relieving hyperuricemia, degrading inosine and / or guanosine, improving intestinal flora composition and reducing inflammatory factors; the lactobacillus plantarum has important significance on preventing, improving and treating gout and hyperuricemia diseases, has high application value and can be applied to preparation of fermented yoghurt.

The invention discloses a compound bacterium enzyme preparation for ensiling and application of the compound bacterium enzyme preparation, and belongs to the field of bacterium enzyme compound preparations. The bacterial enzyme preparation disclosed by the invention comprises lactobacillus plantarum, lactobacillus buchneri, cellulase and xylanase, has the effects of improving the fermentation quality of silage, promoting the improvement of the organism immunity and the anti-stress capability of ruminants and the like, and has wide market value.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

A method for treating silage to enhance the aerobic stability by increasing the fermentation and stabilization of silage is disclosed. The method comprises treating silage or feed with a composition comprising one or more of a Lactobacillus brevis (Levilactobacillus brevis) strain LB7148, deposited as Patent Deposit No. NRRL B-67991 and Lactobacillus buchneri (Lentilactobacillus buchneri) strain LN7149, deposited as Patent Deposit No. NRRL B-67992, including mixtures or mutant(s) thereof which retain their silage preservative activity and / or or the anti-Acetobacter spp. components produced by LB7148 and LN7149, respectively. The strains of Lactobacillus buchneri (Lentilactobacillus buchneri) and Lactobacillus brevis (Levilactobacillus brevis) disclosed herein have been purified, isolated, and when applied to pre-ensiled plant material found to inhibit the growth of Acetobacter spp. and to improve aerobic stability of silage.

The invention relates to a complex microbial inoculant for fermenting and regenerating chicken manure and application of the complex microbial inoculant. The complex microbial inoculant consists of a first complex microbial inoculant and a second complex microbial inoculant which are independently packaged, bacterial strains in the first complex microbial inoculants consist of bacillus coagulans, bacillus subtilis, aspergillus niger and saccharomyces cerevisiae; the bacterial strain in the second complex microbial inoculant is composed of lactobacillus plantarum, lactobacillus acidophilus and lactobacillus buchneri. According to the invention, seven probiotics are combined for use, the first complex microbial inoculant performs aerobic fermentation on a substrate containing chicken manure, unstable factors in chicken manure raw materials are reduced, and a high-quality raw material basis is provided for regenerated feed; wherein the second complex microbial inoculant performs anaerobic fermentation on a substrate containing chicken manure, so that the growth of harmful bacteria is inhibited, the safety of the regenerated product is further ensured, beneficial metabolites are generated, and the palatability of the regenerated product is improved. The finally obtained regenerated feed not only can reduce the feeding cost, but also can regulate the intestinal health, promote the growth of animals and reduce the morbidity.

The invention relates to a preparation method of a leavening agent, in particular to a preparation method and application of a lactic acid bacteria leavening agent suitable for fermenting sweet potatoes. The preparation method of the lactic acid bacteria starter comprises the following steps: respectively and continuously activating lactobacillus plantarum, lactobacillus buchneri and leuconostoc mesenteroides for 2-3 times, compounding an activating solution according to the mass ratio of 1: 1: 1, and fermenting sweet potatoes at the most suitable fermentation temperature of 23.5 DEG C for 3 days with the inoculum size of the starter being 3.5%, thereby ensuring that the strains efficiently ferment the sweet potatoes in the most suitable environment. The preparation method of the lactic acid bacteria starter is simple, low in cost and capable of achieving industrial production, opens up a new way for sweet potato processing and utilization, adds a new variety for the sweet potato product market and promotes value increase and efficiency increase of the sweet potato industry.

The invention belongs to the technical field of animal feed, and discloses a fermented total mixed ration for fattening mutton sheep based on agricultural by-products and a preparation method of the fermented total mixed ration. The fermented total mixed ration is prepared by taking apple pomace, tomato pomace and traditional Chinese medicine residues as main raw materials, matching with high-quality forage grass such as silage corn, alfalfa and a small amount of concentrated feed, regulating and controlling the raw material ratio, inoculating lactobacillus plantarum or lactobacillus buchneri and fermenting under an anaerobic condition. The preparation method is simple in process and good in fermentation stability, the dosage of concentrated feed in daily ration can be effectively reduced, and the feed cost is remarkably reduced. A fattening test verifies that the feed intake, the average daily gain and the feed conversion ratio of mutton sheep can be improved by feeding the fermented total mixed ration disclosed by the invention, meanwhile, the rumen fermentation performance is improved, the antioxidant and immune functions are enhanced, and the dressing percentage and the meat quality are improved. The method realizes efficient feed utilization of agricultural byproducts, and has good economic benefits, ecological benefits and popularization and application values.

The application discloses a high-density culture method of lactobacillus rhamnosus, and belongs to the technical field of microbial fermentation. The high-density culture method comprises the step of adding lactobacillus buchneri to assist the fermentation culture of the lactobacillus rhamnosus. It is first proved that the lactobacillus buchneri is selected as an auxiliary strain to significantly promote the high-density fermentation of the lactobacillus rhamnosus. Compared with the prior art, the mixed fermentation of the lactobacillus buchneri and the lactobacillus rhamnosus can significantly improve the fermentation bacteria number of the lactobacillus rhamnosus by gradually optimizing the fermentation conditions. The product obtained by using the fermentation method has the advantages of high active bacteria number, natural edibility and low cost. The product has a good industrialization prospect.

The present invention discloses a method for preparing lactobacillus fermentation products by two-step combined fermentation of prosopis. The method of the present invention uses organic prosopis powder and arabinose as culture medium, and prepares lactobacillus fermentation products by two-step combined fermentation of prosopis by Lactobacillus buchneri and Lactobacillus plantarum. The present invention preferentially utilizes Lactobacillus buchneri to pre-ferment prosopis powder to promote the release of flavonoids, proteins, alkaloids, and short-chain fatty acids from plant fiber structures, and utilizes Lactobacillus plantarum to further express and modify small molecule active peptides by secondary fermentation. The two-step combination of homotypic fermentation and heterotypic fermentation is adopted to effectively improve the aerobic stability and bioavailability of the fermentation process, and the obtained lactobacillus fermentation product exhibits excellent free radical quenching ability and biological activity against peroxide damage.

This invention relates to the field of Bletilla striata polysaccharide technology, and in particular to a microbial-based method for extracting Bletilla striata polysaccharide. The method includes: pulverizing Bletilla striata medicinal material to obtain powder; adding distilled water to the powder at a material-to-liquid ratio of 1:10-15 and heating to 90°C for 20-30 minutes to obtain a liquid; inoculating the liquid with *Bacillus harzianum* for fermentation to obtain a first fermentation broth; inoculating the sterilized first fermentation broth with *Lactobacillus bruschetta* for fermentation to obtain a second fermentation broth; extracting the second fermentation broth in a boiling water bath for 100 minutes, filtering, and obtaining a polysaccharide aqueous extract; concentrating the polysaccharide aqueous extract to obtain a concentrated polysaccharide aqueous extract; and precipitating the concentrated polysaccharide aqueous extract with alcohol to obtain Bletilla striata polysaccharide. Through two fermentation-assisted treatments, the polysaccharide is less prone to degradation due to high temperatures, and higher temperatures can further increase the polysaccharide dissolution rate of the Bletilla striata powder, resulting in a polysaccharide with stronger antibacterial properties, suitable for use in cosmetics without chemical antibacterial agents.

The application discloses a lactobacillus buchneri with blood sugar reducing activity and application of the lactobacillus buchneri in fermented milk, the strain is lactobacillus buchneri PB6, which is preserved in the Guangdong Microbial Culture Collection Center on May 19, 2025, and the preservation number is GDMCC No: 66352, the strain has blood sugar reducing activity, safety and probiotic property, has good tolerance to gastrointestinal environment, has excellent self-aggregation capacity, antioxidant capacity, and can inhibit the growth of common pathogenic bacteria, and is helpful to maintaining the balance of intestinal flora. The application of the lactobacillus buchneri PB6 in fermented milk can improve the sensory quality and rheological properties of the fermented milk, and has blood sugar reducing activity, rich organic acids and volatile flavor substances, and provides a basis for the application of the strain in functional food.

The invention discloses a preparation method and application of a composition for improving cold resistance of bees. The composition is prepared by performing compound fermentation on Chinese herbal medicine liquid, lactobacillus buchneri, lactobacillus reuteri and bacillus Zhongmuran, the Chinese herbal medicine liquid is prepared from the following raw materials in parts by mass: 9 parts of rhizoma polygoni cuspidati, 30 parts of radix astragali, 15 parts of radix paeoniae alba, 9 parts of radix isatidis and 8 parts of rhizoma dioscoreae nipponicae; the selected probiotics have strong protease production capacity and good antibacterial effect, and are sensitive to various antibiotics. The composition disclosed by the invention can be used for enhancing the immune defense capability of bees in the wintering period, reducing the possibility of disease occurrence and improving the overall survival rate; and the composition has no drug residue while improving the cold resistance of the bees in winter, shows huge sustainable development potential when being applied to bee breeding, and brings economic benefits and social benefits to bee keepers.

The invention discloses a strain of Lactobacillus buchneri for improving GABA content and sleep and application thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is to provide a strain of Lactobacillus buchneri capable of regulating and controlling GABA content and improving sleep, and the key point of the technical scheme is to provide a strain of Lactobacillus buchneri with a preservation number of CGMCC No.37544. The Lactobacillus buchneri is a strain of Lactobacillus buchneri with a preservation number of CGMCC No.37544. The Lactobacillus buchneri JZ-QX01 disclosed by the invention has the capabilities of regulating and controlling the content of GABA (gamma-aminobutyric acid) in a host and improving sleep, and has a huge application prospect in the preparation of a product for regulating and controlling the content of the GABA in the host and regulating the sleep.

The invention discloses a method for degrading African swine fever viruses by fermenting pennisetum sinese with compound probiotics, which comprises the following steps: crushing a pennisetum sinese raw material polluted by the African swine fever viruses; the preparation method comprises the following steps: inoculating a compound probiotic leavening agent containing six specific strains (pichia membranaefaciens C200-1, brevibacillus reuteri RF10-5, pediococcus acidilactici, lactobacillus plantarum, lactobacillus buchneri and bifidobacterium), and fermenting for 6-14 days at 20-40 DEG C under a closed condition. By means of the fermentation process, polluted African swine fever viruses in materials can be effectively degraded, and q-PCR detection shows negative on the seventh day. The method is specially used for solving the problem of African swine fever virus pollution, is clear in effect, green, safe and easy to implement, and provides a feed link solution for prevention and control of African swine fever.

The invention belongs to the technical field of fermented feed, and particularly relates to a compound lactic acid bacterium agent for producing yak feed by fermenting highland barley vinasse, and application and an application method thereof. The invention provides a compound lactic acid bacterium agent for producing yak feed by fermenting highland barley vinasse, which is prepared by compounding bacterium liquid of lactobacillus plantarum, bacterium liquid of lactobacillus buchneri, bacterium liquid of lactobacillus fermentum and bacterium liquid of pediococcus pentosaceus according to the volume ratio of (2-4): (1-3): (1-3): (0.5-2). Cellulose components in the raw materials such as the highland barley vinasse, the alfalfa hay and the rapeseed cake can be fully decomposed, so that the utilization rate of the raw materials is greatly improved, and meanwhile, the content of nutritional ingredients such as crude protein in the fermented feed and the oxidation resistance of the feed are improved; a new way is provided for development of the yak industry and improvement of the additional value of the highland barley vinasse.

The application provides a silage fermentation additive and a preparation method and application thereof, and belongs to the technical field of silage feed. The silage fermentation additive comprises allicin and a compound microbial inoculum, and the compound microbial inoculum comprises lactobacillus acidophilus, lactobacillus plantarum and lactobacillus buchneri. The allicin is compounded with the compound microbial inoculum with a specific composition, and is used for preparing silage feed, so that the aerobic stability of the silage feed can be significantly improved, the growth of spoilage bacteria such as molds and yeasts can be effectively inhibited, the content of various mold toxins such as aflatoxin, zearalenone, T-2 toxin and vomitoxin can be reduced, the fermentation quality can be improved, the protein decomposition can be reduced, and the retention rate of dry matter and water-soluble total sugar can be improved. The application can completely replace chemical preservatives such as potassium sorbate and ammonium propionate, has the characteristics of naturalness, safety and high efficiency, and is suitable for the large-scale production of silage corn, fresh corn straw and other silage feeds.

The application discloses a lactobacillus buchneri and application thereof in plant growth promotion, and belongs to the technical field of microorganisms. Through 16S rRNA sequence analysis, the lactobacillus buchneri HAC1b23.7 isolated is identified as a lactobacillus, and the preservation number is CGMCC No. 28790. The fermentation liquor of the lactobacillus buchneri HAC1b23.7 strain has a broad-spectrum promoting effect on the planting and growth of crops, and can also enhance the transformation and absorption of nutrients in soil by plants. The lactobacillus buchneri HAC1b23.7 can be prepared into a microbial preparation and applied to the field of plant growth promotion, and has a wide prospect.

The invention discloses a lactobacillus buchneri strain capable of fermenting and converting p-hydroxybenzyl alcohol in bamboo shoots into sour bamboo shoot characteristic flavor substances, and belongs to the field of probiotic fermentation, the lactobacillus buchneri strain CCFM1421 is screened and preserved in Guangdong Microbial Culture Collection Center on October 31, 2024, and the preservation number is GDMCC No: 65377. The lactobacillus buchneri CCFM1421 screened by the invention has the characteristics of acid resistance and salt tolerance, p-cresol can be prepared by fermenting p-hydroxybenzyl alcohol, and a high-quality strain resource is provided for targeted regulation and control of the flavor of sour bamboo shoots and development of a sour bamboo shoot leavening agent.

The application discloses a compound lactic acid bacteria additive for improving the quality of corn straw-sweet potato vine compound silage and application thereof, and belongs to the field of feed processing. The compound lactic acid bacteria additive comprises the following components in parts by weight: 0.5-1.5 parts of lactobacillus plantarum and 0.8-1.2 parts of lactobacillus buchneri. The compound lactic acid bacteria additive can significantly improve the crude protein content of corn straw-sweet potato vine compound silage, promote the degradation of fiber components to improve the digestion and utilization rate, quickly reduce and maintain the low pH value of the silage, reasonably accumulate lactic acid, inhibit the generation of butyric acid and the accumulation of ammonia nitrogen, maintain a high propionic acid content to prolong the aerobic stable period of the feed, and has better effects than traditional silage additives in terms of fiber degradation, protein retention, fermentation environment regulation and the like, so that the research blank of the special additive for the compound raw material silage is filled, and the resource utilization of agricultural wastes is realized.

The invention belongs to the technical field of microorganisms, and discloses a microbial composition and application thereof in degradation of antibiotics. The microbial composition for meat antibiotic degradation is designed according to the household use scene, spiramycin (SPM) left on the surface and deep layers of meat is rapidly removed through biodegradation of microorganisms and metabolites of the microorganisms, and the household diet safety pain point is solved. The selected microorganisms comprise bacillus safensis, bacillus tequilensis, bacillus subtilis, bacillus pumilus and lactobacillus buchneri, the microorganisms belong to probiotics, and new potential food safety hazards cannot be caused after livestock and poultry meat is treated; in addition, the selected auxiliaries are conventional additives in the food field, so that the edible safety of the treated meat is ensured. The degradation rate of the microbial composition on SPM in pork reaches 99% or above, and the effect is remarkable.

The invention relates to a health-care composition containing a tiger-claw ginger fermentation product and a preparation method of the health-care composition, and belongs to the technical field of health-care products. The health-care composition comprises a tiger-claw ginger fermentation product, a composite natural extract and a sweet additive, the preparation method of the tiger-claw ginger fermentation product comprises the following steps: fermenting lactobacillus buchneri / lactococcus lactis subsp. Lactis / tiger-claw ginger, separating filtrate and filter residues, carrying out secondary extraction on the filter residues, separating filtrate again, merging the two filtrates, and purifying; the composite natural extract consists of a mung bean extract, a fructus rosae laevigatae extract, a mulberry leaf extract and a chamomile extract; the health-care composition has an excellent lipid-lowering effect, belongs to a brewing type health-care product, is convenient to take and is suitable for daily life.

The invention discloses lactobacillus buchneri and application thereof, and relates to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center on November 28, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.32833. The strain has efficient nucleoside degradation capacity, can completely degrade nucleoside within 30 minutes, shows excellent acid resistance and cholate tolerance, is high in survival rate in an in-vitro simulated digestion environment, is sensitive to antibiotics, does not have hemolytic activity, has remarkable uric acid reduction capacity, can relieve hyperuricemia, and has the effects of improving blood lipid metabolism, reducing blood fat and reducing blood fat. The strain has outstanding performance in the aspects of repairing kidney injury caused by hyperuricemia, relieving fatty liver and inhibiting inflammation, can promote generation of short-chain fatty acid and regulate the intestinal flora structure, can be used for prevention and adjuvant therapy of hyperuricemia and metabolic diseases thereof, and has wide market application prospects in the nutrition and health industry.

The invention discloses a lactobacillus buchneri fermentation culture process which comprises the following steps: activating a lactobacillus buchneri strain, preparing a seed solution, inoculating 1%-5% of the seed solution into a fermentation formula after the seed solution is cultured, culturing for 20-30 hours at 36 + / -1 DEG C to obtain a fermentation solution, then sucking 1-10 mL of the fermentation solution into 99-90 mL of 0.3% of sterile tween-80 water, diluting to a proper gradient, and culturing for 2-3 hours at the temperature of 36 + / -1 DEG C to obtain the lactobacillus buchneri fermentation culture process. The lactobacillus buchneri fermentation liquor has the advantages that the lactobacillus buchneri fermentation liquor is prepared by the aid of the lactobacillus buchneri fermentation liquor fermentation process, 1mL of lactobacillus buchneri fermentation liquor is sucked into a culture dish, 15mL of MRS detection culture media is added into the culture dish and uniformly mixed, the lactobacillus buchneri fermentation liquor is cultured in an incubator at 36 + / -1 DEG C for 48h, and the viable count of lactobacillus buchneri in the lactobacillus buchneri fermentation liquor obtained by the lactobacillus buchneri fermentation process is larger than or equal to 100 * 10 < 8

The present application relates to the technical field of feed processing, in particular to a compound feed additive of broccoli picking residues and its preparation method and application. The broccoli stem and leaf picking residues and safflower petals are mixed and crushed, and then inoculated with a compound probiotic strain composed of Lactobacillus mucosae ZUST-12, Lactobacillus plantarum ZUST-49 and Lactobacillus buchneri ZUST-18 after adding water, and then subjected to anaerobic fermentation. The fermentation liquor is filtered to obtain the fermented juice of broccoli and safflower picking residues. The fermented juice of broccoli and safflower picking residues is concentrated and then spray dried to obtain the compound feed additive of broccoli picking residues. The present application develops a new feed additive preparation method which can integrate two kinds of high-activity agricultural waste, and synergistically amplify the functional components of immunity by specific fermentation technology. The present application has innovative value for filling the gap in the prior art and realizing high-value utilization of agricultural waste.

The present disclosure discloses multifunctional lactobacilli capable of relieving a toxic effect of perfluorooctanoic acid (PFOA) and use thereof, and belongs to the technical field of microorganisms. Lactobacillus fermentum CCFM1051, Lactobacillus casei CCFM1052 and Lactobacillus buchneri CCFM1053 of the present disclosure have high adsorption effect on PFOA, can significantly relieve liver oxidative stress damage and serum biochemical indicators caused by PFOA, spleen atrophy caused by PFOA exposure, imbalance of intestinal microorganisms caused by PFOA exposure and metabolic disorder of intestinal flora caused by PFOA exposure, significantly increase the content of acetic acid and propionic acid in the intestinal tract, increase the fecal water content and decrease first black stool defecation time of mice with constipation, improve proliferation and MafA gene expression of INS-1 cells under high glucose, have the potential to relieve PFOA-related diabetes, reduce occurrence of liver diseases, metabolic diseases and potential carcinogenicity, and have broad use prospects.

The invention relates to zein-based cinnamyl aldehyde-curcumin composite nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of food preservation. The technical problems that bag expansion, browning and the like easily occur in the storage process of the pickled Chinese cabbages are solved. According to the zein-based cinnamyl aldehyde-curcumin composite nanoparticles disclosed by the invention, zein is taken as a nano carrier, cinnamyl aldehyde and curcumin are taken as loaded drugs, and sodium alginate is taken as a stabilizer. The zein-based cinnamyl aldehyde-curcumin composite nanoparticles can effectively inhibit the growth of aerogenic bacteria-Delftia acidovorans and Lactobacillus buchneri in the storage process of pickled Chinese cabbages, and can synchronously achieve the triple effects of enhancing the antioxidant activity of the pickled Chinese cabbages, improving the color and luster of the pickled Chinese cabbages and maintaining nutritional ingredients; the quality deterioration in the storage process is effectively delayed.

The application discloses a lactobacillus buchneri complex microbial agent and application thereof, and belongs to the technical field of microorganisms. In order to solve the problem that the direct degradation effect of lactic acid bacteria on uric acid is poor in the prior art, the application provides a complex microbial agent containing lactobacillus buchneri NEFU-1 and NEFU-2, and the complex microbial agent has the functions of degrading uric acid, relieving hyperuricemia, degrading inosine and / or guanosine, improving intestinal flora composition and reducing inflammatory factors; the complex microbial agent has important significance for preventing, improving and treating gout and hyperuricemia diseases, has high application value, and can be applied to preparation of fermented yoghourt.

The invention discloses oat straw silage and a preparation method thereof. The preparation method of the oat straw silage provided by the invention comprises the following steps: spraying a microbial agent into oat straws, and fermenting to obtain the oat straw silage, the microbial agent comprises lactobacillus buchneri, lactobacillus plantarum and lactobacillus fermentum. According to the method disclosed by the invention, the high-quality oat straw silage product is obtained by inoculating lactic acid bacteria additives with different functions, so that the feeding value of oat is improved, and a theoretical basis is provided for popularization of the high-quality oat straw silage.

The invention discloses a compound probiotic leavening agent, a preparation method thereof and a pre-fermented fungus grass feed prepared from the compound probiotic leavening agent. The preparation method of the compound probiotic leavening agent comprises the following steps: mixing pichia membranaefaciens C200-1 and brevibacillus reuteri RF10-5 in proportion to obtain aerobic mixed bacteria, mixing pediococcus acidilactici, lactobacillus plantarum, lactobacillus buchneri and bifidobacterium in proportion to obtain anaerobic mixed bacteria, and compounding the aerobic mixed bacteria and the anaerobic mixed bacteria in a proportion of 1: (45-55) to obtain a seed solution. The seed solution is diluted by molasses for expanding cultivation, then is sprayed on crushed pennisetum sinese (such as pennisetum sinese), and is fermented under a closed condition to obtain the pre-fermented pennisetum sinese feed. The pre-fermented fungus grass feed is used for feeding pigs, can remarkably improve the feed utilization rate, regulate intestinal flora, enhance immunity, reduce the feed conversion ratio and improve the breeding environment, and provides an effective technical scheme for replacing grains with grass and saving grains.