140 results about "Lactobacillus buchneri" patented technology

The invention relates to a dry powder ready-to-use starter and a preparation method for the starter. The dry powder ready-to-use starter consists of the compound lactic acid powder made from lactobacillus plantarum 550 and lactobacillus buchneri 225, the nutritional agents promoting the lactobasillus to grow or perfume selectively added. The lactobacillus plantarum powder and the lactobacillus buchneri powder are prepared by liquefying, saccharifying, inoculating, fermenting, centrifugating, spraying and drying the corn powder. The compound lactobacillus powder is prepared by mixing the lactobacillus plantarum powder with the lactobacillus buchneri powder by a weight ratio of the lactobacillus plantarum powder to the lactobacillus buchneri powder ranging from 65 to 30: 70 to 35. When the infusing is carried out, the compound lactobacillus powder is dissolved into the fermentation broth by the cold boiling water and the salt, the sugar, the peptone and the perfume are added to the fermentation broth to make the pickle. The pickle products can be obtained by infusing the fresh vegetables in the pickle for 18 to 24 hours. With easy storage, convenient carrier and use, quick and simple preparation, the starter mannufactures the pickle which not only reaches the flavor and quality of the traditional products, but also effectively lowers the formation of the nitrites.

The invention discloses an inoculant containing homofermentation malolactic bacteria and heterofermentation malolactic bacteria for corn silage and using method thereof. The composite microorganism inoculant for corn silage includes 2*10<9>cfu / g-5*10<10>cfu / g of lactobacillus buchneri and 5*10<9>cfu / g-1*10<11>cfu / g of lactobacillus plantarum. The composite microorganism inoculant for corn silage provided by the present invention employs the homofermentation malolactic bacteria and the heterofermentation malolactic bacteria as acid generation source, thereby increasing preservation effect of corn silage forage. When silage forage prepared from the inoculant of the present invention is used as forage of cow, milk yield and butterfat rate can be increased.

The invention provides a lactic acid bacteria agent suitable for grass silage. The lactic acid bacteria agent consists of lactobacillus plantarum lyophilized powder and lactobacillus buchneri lyophilized powder, wherein the lactobacillus plantarum and the lactobacillus buchneri are both separated from ryegrass straw; the lactobacillus plantarum is homofermentative lactic bacteria, and the lactobacillus buchneri is heterofermentative lactic bacteria; the two strains are collected in the China Center for Type Culture Collection on May 25th, 2016; the strain of the lactobacillus plantarum is L.plantarumZRR, and the collection number is CCTCC No:M2016281; and the strain of the lactobacillus buchneri is L.buchneriBR2, and the collection number is CCTCC No:M2016280. The lactic acid bacteria provided by the invention has the advantages of accelerating grass silage maturing, improving grass silage quality, increasing aerobic stability and the like and can be widely applied to the field of grass silage feed preparation.

A method for treating silage to enhance aerobic stability by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri, LN 1326, or the antimicrobial components produced thereby. The strain of Lactobacillus buchneri disclosed in the invention has been purified and isolated and has been found to be nontoxic, safe and able to improve aerobic stability of silage.

The invention relates to pickled vegetable salt containing single or compound lactobacillus and a preparation method thereof, belonging to the field of biotechnology and food. The pickled vegetable salt is prepared by salt, glucose, single or compound lactobacillus, calcium lactate and compound spices, wherein the single lactobacillus is selected from lactobacillus. plantaerun, lactobacillus. brevis, lactobacillus. fermentium, lactobacillus. coryniformis, lactobacillus. buchneri, lactobacillus. casei or leuconostoc. mesenteroides; and the compound lactobacillus is a mixture formed by two or more above single lactobacillus according to any weight ratio. The pickled vegetable salt prepared by the invention not only can be used for initially preparing pickled vegetable salt water, but also can supplement lactobacillus and nutrients formed by lactobacillus growth and the products while supplementing salt in the process of vegetable pickling and then keep the content of lactobacillus in the pickled vegetables at a higher level, and the prepared pickled vegetables have good mouthfeel and meet the requirement during preparing the pickled vegetables by people in different regions.

A method for treating silage to enhance aerobic stability by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri, LN1297, or the antimicrobial components produced thereby. The strain of Lactobacillus buchneri disclosed in the invention has been purified and isolated and has been found to be nontoxic, safe and able to improve aerobic stability of silage.

The invention discloses a biological feed additive capable of effectively enhancing immunity of piglets, which belongs to the field of a feed additive. The additive comprises a compound Chinese herbal medicine and a micro-ecologic preparation, wherein the Chinese herbal medicine comprises pseudo-ginseng, dendrobe, cacumen biotae, eucommia ulmoides, rhodiola rosea, officinal magnolis flower, astragalus membranaceus, platycodon grandiflorum, orange peel, burdock, isatis root and sea-buckthorn; the micro-ecologic preparation is composed of bacillus subtilis, bacillus licheniformis, bifidobacterium, lactobacillus buchneri and pediococcus acidilactici. Due to the adoption of the feed additive, the growth performance of piglets can be improved, and the immunity of the piglets can be enhanced at the same time, and furthermore, the feed additive has a very wide market prospect.

The invention provides an enzyme bacterium composite additive used for fermentation of straw green / yellow silage. The enzyme bacterium composite additive comprises leuconostoc mesenteroides, lactobacillus plantarum, lactobacillus buchneri, cellulose, xylanase, and beta-glucanase. The enzyme bacterium composite additive is sprayed onto straw for straw green / yellow silage fermentation, is capable of destroying a special lignin-cellulose-hemicellulose composite structure in straw green / yellow silage effectively, improving fermentation quality of straw green / yellow silage, increasing preservation amount of nutrients, increasing degradation rate of the rumen of ruminants on straw nutrients, and increasing utilization rate of straw. The enzyme bacterium composite additive can be dissolved in water directly for spraying on straw for green / yellow silage fermentation, is convenient to use, and possesses a promising application prospect.

The invention discloses an acetic acid type pickled Chinese cabbage composite leavening agent, a preparation method thereof and an application, and belongs to the technical field of vegetable processing. The acetic acid type pickled Chinese cabbage composite leavening agent mainly comprises bacterial powder and calcium chloride; and the bacterial powder consists of acetobacter pasteurianus, leuconostoc mesenteroides, lactobacillus plantarum, lactobacillus brevis, lactobacillus buchneri and lactobacillus acidophilus. The leavening agent has the advantages of high activity, good taste, convenience in use and reliable quality. The viable count of the acetobacter pasteurianus, the leuconostoc mesenteroides, the lactobacillus plantarum, the lactobacillus brevis, the lactobacillus buchneri and the lactobacillus acidophilus reaches 1010cfu / g. After being fermented by the acetic acid type pickled Chinese cabbage composite leavening agent, Chinese cabbages contain lactic acid which tastes soft, and also contains flavor substances such as acetic acid tasting sour, and the taste of the Chinese cabbages which are fermented by the acetic acid type pickled Chinese cabbage composite leavening agent is the same with that of pickled Chinese cabbages which are fermented naturally by the traditional method.

The invention provides a granulation-resisting lactic acid bacterium preparation and a preparation method thereof. The granulation-resisting lactic acid bacterium preparation comprises lactic acid bacterium; the lactic acid bacterium is one or a combination of more of enterococcus faecalis, enterococcus faecium, lactobacillus plantarum, lactobacillus acidophilus, lactobacillus lactis, lactobacillus buchneri, lactobacillus casei and bifidobacterium animalis; a viable count of the lactic acid bacterium reaches 1.0*10<10> to 1.0*10<11>CFU / g. According to the preparation method of the lactic acidbacterium preparation, provided by the invention, the tolerance of thalli is improved and the loss of bacterium activity in a processing process is avoided.

A method for treating silage to enhance aerobic stability by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri, LN5665, or the antimicrobial components produced thereby. The strain of Lactobacillus buchneri disclosed in the invention has been purified and isolated and has been found to be nontoxic, safe and able to improve aerobic stability of silage.

The invention discloses a method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum. The method comprises the steps of cutting mowed sugarcane tails into short pieces with the lengths of 1-4cm; evenly spraying the mixed preparation of the lactobacillus plantarum and the lactobacillus buchneri onto the fresh sugarcane tail raw material to enable the viable count of the lactobacillus plantarum and the lactobacillus buchneri in the fresh sugarcane tails to reach 1*106cfu / g-2*106cfu / g, evenly mixing, filling, compacting and sealing, wherein the density of the mixture is 0.4kg / L; storing the product for 40 days at the normal temperature to obtain the sugarcane tail silage. After the method is adopted, the problem of poor palatability caused when a ruminant is directly fed with the sugarcane tails can be solved; the lactobacillus buchneri is combined with the lactobacillus plantarum for application, so that the content of lactic acid is improved, the content of acetic acid is reduced, and the utilization efficiency of water soluble carbohydrate (WSC) of the silage is improved.

Embodiments of the present invention include polypeptides having ferulate esterase activity and the nucleic acid sequences that encode them. Methods of the embodiments utilize these ferulate esterase polypeptides and nucleic acid sequences to enhance the digestibility of plant cell walls and the accessibility of carbohydrates in plants. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulate esterase from Lactobacillus buchneri. Methods of using same to enhance plant fiber digestion in animals, are disclosed. Uses of this invention include, but are not limited to, forage and silage with improved digestibility for livestock, and enhanced biomass conversion.

The invention discloses a preparation method of a bacterial fertilizer or a bacterial product. The preparation method is characterized by comprising the following steps: uniformly mixing a type A microbial agent and a type B microbial agent in the ratio of 1 to (0.1 to 10); adding the uniformly-mixed microbial agent into a pesticide residue culture medium to obtain the bacterial fertilizer or thebacterial product. Active components of the microbial agent consist of rhizobium japonicum, bacillus megaterium, bacillus cereus, bacillus subtilis, bacillus mucilaginosus, lactobacillus buchneri, bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus pumillus, bacillus licheniformis, trichoderma harzianum, etc. The microbial agent and pesticide residues are mixed uniformly to obtain the bacterial fertilizer or the bacterial product. The bacterial fertilizer or the bacterial product can be applied to the facilitation of crop growth or the improvement of crop yield or the improvement of planting soil, as well as the improvement of crop disease resistance. The product can be combined with different bacterial fertilizer carriers to form a solid bacterial fertilizer, a dry or gel starchbacterial fertilizer, a liquid bacterial fertilizer, a pesticide residue waste bacterial fertilizer, a grass charcoal bacterial fertilizer, a peat bacterial fertilizer, a hibernated bacterial fertilizer, as well as a pig, cattle, sheep and chicken manure excrement bacterial fertilizer, and to form seed coatings.

A method for treating silage to enhance aerobic stability by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri, LN5689, or the antimicrobial components produced thereby. The strain of Lactobacillus buchneri disclosed in the invention has been purified and isolated and has been found to be nontoxic, safe and able to improve aerobic stability of silage.

The invention discloses a straw treatment microecological preparation and a preparation method thereof. The straw treatment microecological preparation comprises digestive lactobacillus, lactobacillus fructivorans, lactobacillus buchneri, bacillus pumilus, bacillus polymyxa and bacillus coagulans. The preparation method comprises the following steps of carrying out mixed culture on three lactic acid bacteria: the digestive lactobacillus, the lactobacillus fructivorans and the lactobacillus buchneri which are separated, identified and screened from a silage fermentation system and contain homofermentative lactic bacteria and heterofermentative lactic bacteria; carrying out the mixing culture on three fibrolytic bacteria: the bacillus pumilus, the bacillus polymyxa and the bacillus coagulans which are from the nature; mixing two mixtures into a composite product, wherein the microbial storage inoculation amount of every ton of straws is 1 L. The straw treatment microecological preparation disclosed by the invention can be used for enhancing the silage quality and the aerobic stability and reducing the loss of weight in a fermentation process by reducing pH and protein degradation rate and is very significant to animal husbandry production by reducing the decomposing speed of a silage after the silage is fed by opening a cellar.

A method for treating silage to enhance aerobic stability by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri, LN1284, or the antimicrobial components produced thereby. The strain of Lactobacillus buchneri disclosed in the invention has been purified and isolated and has been found to be nontoxic, safe and able to improve aerobic stability of silage.

The present invention is directed to methods and compositions for typing of Lactobacillus buchneri bacterial strains, detecting the presence of a L. buchneri in a sample, identifying a strain of L. buchneri having resistance to an invasive foreign genetic element, modifying the resistance of bacteria and archeae to an invasive foreign genetic element, and introducing nicks into or cleaving double stranded DNA for genome editing.

The invention discloses a pig feed additive for improving intestinal health and boosting immunity and belongs to the field of feed additives. The additive is prepared from compound plant polysaccharide, complex enzyme preparation, lactobacillus plantarum powder, lactobacillus buchneri powder, stevia rebaudiana powder, fructus chebulae powder, orange peel powder, arginine, glutamine dipeptide, alpha-oxoglutarate, cystine, taurine and a carrier, wherein corncob powder and peanut shell powder serve as the carrier. By the adoption of the additive, pig intestinal health can be improved, intestine development of live pigs can be promoted, the feed digestibility and absorptivity of live pigs can be improved, pig growth can be promoted, marketing period can be shortened, economical benefits can be increased, and feeding effect can be improved. Meanwhile, the immunity of pigs can be improved, the morbidity of pigs can be reduced, and application prospects are quite broad.

The invention provides a composite strain fermentation agent and a preparation method of silage. The composite strain fermentation agent comprises the following components: lactobacillus plantarum, enterococcus faecium and lactobacillus buchneri. By adding the composite strain fermentation agent when whole-crop corns are silaged, nutritional ingredients in silaged corns can be well kept, a pH value of the silaged whole-crop corns is rapidly reduced, content of ammoniacal nitrogen is reduced, lactic acid content is improved, a lactic acid fungus quantity is increased, and quality of the silaged whole-crop corns is improved. An experimental result shows that the silaged whole-crop corns obtained by adding the composite strain fermentation agent provided by the invention has the pH value of smaller than or equal to 4.0, mass content of lactic acid of greater than or equal to 7.5% and mass content of ammoniacal nitrogen of smaller than or equal to 2.5%.

The invention discloses a lactobacillus buchneri inoculant belonging to the technical field of quality safety of silage as well as a preparation method and an application of the lactobacillus buchneri inoculant. The lactobacillus buchneri inoculant is prepared from lactobacillus buchneri and dried skim milk, wherein the content of the lactobacillus buchneri in the lactobacillus buchneri inoculant is 1*10<10>-5*10<10> CFU / g. Lactobacillus buchneri thalli and a solution containing the dried skim milk are mixed and freeze-dried into powder under a vacuum freezing condition, and the inoculant is prepared. When applied to preparation of whole corn silage, the inoculant can improve the aerobic stability of the whole corn silage remarkably, inhibits mould proliferation in an aerobic spoilage process, reduces the content of aflatoxin B1 and improves the performance of the whole corn silage. The preparation method of the lactobacillus buchneri inoculant is simple and feasible, and the lactobacillus buchneri inoculant has wide application prospect in the technical field of quality safety of the silage.

The invention provides a processing method of ramie and pennisetum purpureum mixed silage, and belongs to the field of feed additives. The processing method comprises the following specific steps: mixing 60%-80% of pennisetum purpureum and 20%-40% of ramie to obtain silage; then spraying fermentation liquor to the silage, spraying an additive at the same time, and then performing fermentation. Thefermentation liquor comprises compound bacteria, compound enzyme and water, and the compound bacteria comprise lactobacillus plantarum, lactobacillus buchneri, pediococcus pentosaceus and bacillus subtilis; the compound enzyme comprises cellulase, xylanase and laccase; and the additive comprises calcium propionate, molasses, urea, ethanol and water. According to the processing method of the silage, ramie and pennisetum purpureum mixed silage is adopted, nutrients are complemented, the fermentation liquid and the additive are combined for fermentation, the defect that ramie and pennisetum purpureum silage is difficult to silage is overcome, the dry matter and crude protein content and lactic acid bacteria of the silage are increased, and the crude ash content, the acid washing fiber content, the lignin content and the pH value are reduced.

The invention discloses GS microbial inoculum for disposing life wastewater and the application of the GS microbial inoculum and belongs to the field of biotechnology. The GS microbial inoculum is constituted by, by weight, 3-9 parts of bacillus, 24-48 parts of lactic acid bacteria and 12-24 parts of nitrobacteria, wherein the bacillus is constituted by methyl trophism bacillus, bacillus subtilis and bacillus amyloliquefaciens, and the lactic acid bacteria are constituted by lactobacillus casei and lactobacillus buchneri. According to the GS microbial inoculum for disposing the life wastewater and the application of the GS microbial inoculum, through identification and safety analysis on bacterial strains, reasonable compatibility is conducted on the bacillus, the lactic acid bacteria and the nitrobacteria, and the GS microbial inoculum for disposing the life wastewater is obtained. After the life wastewater is disposed through the GS microbial inoculum, the removal rate of COD and the removal rate of NH<4>-N reach over 90%.

The invention belongs to the technical field of biological active bacterial preparations, particularly relates to a probiotic composition capable of obviously preventing and relieving the Alzheimer'sdisease, and further discloses a preparation prepared from the probiotic composition for preventing and relieving the Alzheimer's disease. The probiotic composition capable of obviously preventing andrelieving the Alzheimer's disease comprises lactobacillus plantarum Lp3a, bifidobacterium lactis Bla019, bifidobacterium longum BL5b, lactobacillus buchneri Lbu3, propionibacterium freudenreichii PrF01, propionibacterium shermanii, pediococcus acidilactici, clostridium butyricum and lactobacillus fermenti. The probiotic composition not only can obviously regulate and maintain intestinal microecology and control an in vivo toxin level but also can generate a certain quantity of short chain fatty acid, including acetic acid, propionic acid, butyric acid, lactic acid and the like to further relieve the related symptoms of the Alzheimer's disease. The probiotic composition has the efficacy of alleviating and relieving the symptoms of the Alzheimer's disease, also has the efficacy of soothingthe nerves and calming down, and also has a certain function for the depression, the anxiety disorder and the insomnia.

The invention relates to the technical field of cultivation, and discloses a disease-resistance improving feed of laying hens. The disease-resistance improving feed is prepared from corn, wheat bran, green grass, black soya bean meal, kelp residues, tea leaf residues, shaddock peels, mango seeds, wool, pig blood, beer fermentation residues, egg shells, vermiculite powder, medical stone powder, composite vitamins, compound amino acid, folium ginkgo polysaccharide, algal polysaccharides, salt and lactobacillus buchneri. The sick rate is reduced to be 6% to 7%, the death rate is 0%, and the economic income is increased by 17.3%; the feed is rich in nutrient, the immunity of laying hens is enhanced, and the price is low; the wool is frozen, boiled and fermented with the lactobacillus buchneri, peculiar smells can be eliminated, palatability can be improved, nutrient absorption can be promoted, and the gastrointestinal functions can be protected; as the egg shells, the vermiculite powder and the medical stone powder can provide rich mineral elements, the laying rate can be increased, the adoption approaches of the egg shells can be increased, and the cultivation income can be increased; when the feed is fractionally added, the absorption and utilization rate is high, the cultivation rate is saved, and the disease resistance is improved.

The invention discloses a decomposed solid fermentation fungicide using agricultural organic wastes such as cow dung and straws (walnut pruning branches and cotton stalks) as raw materials, includingfive bacteria of saccharomyces cerevisiae, bacillus methylotrophicus, geobacillus thermodenitrificans, bacillus simplex and lactobacillus parabuchneri. The invention also discloses a method for preparing the decomposed solid fermentation fungicide. The active fungicide utilizes the synergistic effect between the five bacteria in the composting materials to rapidly form a dominant flora and fully exerts the deodorization and decomposing process promoting effects. At the same time, the problem that the effective utilization rate of the agricultural organic wastes is not high, the natural fermentation composting time is long, and the product quality is poor is solved. A fertilizer subjected to decomposed fermentation can improve the soil fertility of the farmland, regulate the pH of the soil,and has good ecological and economic benefits.

The present invention provides a feed silage agent containing Lactobacillus buchneri, wherein the silage agent comprises Lactobacillus buchneri, Lactobacillus plantarum and Bacillus coagulans, the Lactobacillus buchneri is preserved in the China General Microbiological Culture Collection Center on August 9, 2017, has the preservation number of CGMCC NO.14508, and has the classification name of Lactobacillus buchneri, the Lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center on May 27, 2016, has the preservation number of CGMCC NO.12554, and has theclassification name of Lactobacillus plantarum, and the Bacillus coagulans is preserved in the China General Microbiological Culture Collection Center on on May 27, 2016, has the preservation numberof CGMCC No.12553, and has the classification name of Bacillus coagulans. According to the present invention, with the application of the feed silage agent containing Lactobacillus buchneri in the whole-plant corn silage, the pH value of the corn silage can be significantly reduced, the contents of acetic acid and lactic acid can be obviously increased, and the content of lactic acid bacteria canbe obviously improved.

The invention discloses a crop straw silage leavening agent, prepared from the following raw materials in percentage by weight: 50-60% of a microbial strain, 15-25% of a protective agent, 1-5% of a compound additive, 5-15% of carrier defatted rice bran, and 5-15% of light calcium carbonate, wherein the microbial strain is obtained by mixing of following bacterial powder in percentage by weight: 20-25% of lactobacillus plantarum, 20-25% of pediococcus acidilactici, 10-20% of lactobacillus acidophilus, 10-15% of enterococcus faecalis, 10-15% of lactobacillus buchneri, and 10-15% of lactobacillus paracasei. The leavening agent disclosed by the invention can be applied to preparation of a crop straw silage, and the invention also discloses a preparation method of the crop straw silage. The leavening agent disclosed by the invention can be used for fermenting various crop straws into available feed products, antibiotics are not used at all, and the digestivity to feeds of animals is improved, so that the crop straws are changed from waste into valuable, and the cost of raising livestock is reduced.

The invention relates to a lactobacillus buchneri for producing mannitol. The lactobacillus buchneri is called lactobacillus buchneri Y-4, named lactobacillus buchneri by classification, and preserved on 13, March, 2013 with preservation number of CGMCC NO.7300. The lactobacillus buchneri is characterized in that northeast sauerkraut is taken as the raw material, the lactobacillus buchneri is obtained by using an enrichment culture method, a preliminary screening method, a re-screening method and other methods for screening and purification, and the strain is determined as a new species according to the physiological and biochemical properties and 16 SrD DNA sequence analysis. The obtained strain has stable growth performance, the yield of mannitol is highest at a temperature of 30 DEG C and pH of 5.0, and the lactobacillus buchneri has a good research and application prospect.

The invention discloses multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof. The lactobacillus buchneri CCFM1053 can adsorb PFOA, improve spleen atrophy caused by PFOA exposure, increase content of TNA-alpha in serum after PFOA exposure, increase content of glutamic-pyruvic transaminase, glutamic oxalacetic transaminaseand gamma-glutamyl transpeptidase in serum in mice after PFOA exposure, reduce the content of MDA in liver after PFOA exposure to the normal level, reduce GAH activity, improve intestinal flora disorder, reduce hepatopathy, improve excrement moisture content and first melena discharge time of constipation patients, relieve constipation, improve proliferation of INS-1 cells and expression of MafA genes under the high glucose action and relieve PFOA related diabetes mellitus.