11 results about "Issatchenkia orientalis" patented technology

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention discloses issatchenkia orientalis and application thereof. Relates to the technical field of fermentation. The invention provides a specific strain and application thereof in reconstituted tobacco. According to the tobacco leaf extracting solution fermented by the bacterial strain, the content of esters such as 2, 2, 4-trimethylpentanediol isobutyl ester, 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, tributyl phosphate and methyl palmitate is remarkably increased, eight novel ester substances with the characteristic aroma such as fruit aroma and rosin are newly generated, and the tobacco leaf extracting solution has the advantages that the content of the esters such as the 2, 2, 4-trimethylpentanediol isobutyl ester, the 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, the tributyl phosphate and the methyl palmitate is increased; and the whole fragrance style is obviously enhanced. It is shown that the aroma-producing issatchenkia orientalis IO-1 fermented tobacco leaf extracting solution has good aroma-producing capacity, and through the combined effect of enzymolysis and fermentation, the extracting solution is expected to play an important application value in the field of reconstituted tobacco leaf raw material quality improvement.

The present application relates to a kind of heat-resistant isayama orientalis engineering bacteria and its construction method and application, belong to genetic engineering technical field.The heat-resistant isayama orientalis engineering bacteria uses isayama orientalis as starting strain, and overexpresses DnaK gene, DnaJ gene and GrpE gene.The present application integrates thermophilic bacteria molecular chaperone system in isayama orientalis, can improve the heat-resistant performance of isayama orientalis, and engineering bacteria can grow and ferment stably under high temperature condition, with high safety and economy.

The invention discloses issatchenkia orientalis capable of efficiently synthesizing L-malic acid and application of the issatchenkia orientalis, and belongs to the technical field of biological engineering. In an existing gene edited yeast strain in a laboratory, endogenous lactic dehydrogenase IoLDH2 and IoLDH3 are over-expressed, endogenous IoLDH1 is knocked out, and lactic dehydrogenase EcD-LDH from escherichia coli and lactic dehydrogenase NmD-LDH from neisseria meningitidis are over-expressed. After fermentation for 72 h in a 5 L fermentation tank, the accumulation amount of L-malic acid reaches 296.27 g / L, the yield is 0.97 g / g, the production intensity is 4.11 g / L / h, and the by-product lactic acid is remarkably reduced from 23.56 g / L to 6.7 g / L and is reduced by 71.56%.

The invention relates to the technical field of biological active peptides, in particular to camel milk-derived immunoactive peptide for adjuvant therapy of tumors and an extraction method and application thereof.The camel milk-derived immunoactive peptide comprises 32 peptide fragments, the sequences of the peptide fragments are shown as SEQ ID NO: 1-SEQ ID NO: 32, and the camel milk-derived immunoactive peptide is obtained according to the following method: culturing probiotics including lactic acid bacteria and saccharomycetes; the lactic acid bacteria are lactobacillus kefiranofaciens, lactobacillus acidophilus and lactobacillus pentosus, and the saccharomycetes are issatchenkia orientalis; preparing fermented camel milk, and inoculating probiotics into the sterilized and degreased camel milk to obtain the fermented camel milk; and extracting and separating the biological active peptide in the obtained fermented camel milk to obtain the camel milk-derived immunoactive peptide for adjuvant therapy of tumors. The camel milk-derived immunocompetent peptide can be rapidly and effectively extracted from fresh camel milk, and the camel milk-derived immunocompetent peptide is identified and subjected to target determination, so that it is verified that the camel milk-derived immunocompetent peptide has a proliferation effect on RAW264.7 cells, and the camel milk-derived immunocompetent peptide can be applied to prevention or / and antitumor drugs.

The invention discloses a method for preparing tea fermentation liquor through multi-bacteria mixed fermentation, the tea fermentation liquor and application of the tea fermentation liquor. The method comprises the steps that tea powder and a fermentation substrate are mixed and sterilized, and a pre-fermentation system is obtained; a macro fungus fermentation stage: inoculating the pre-fermentation system with the schizophyllum commune seed solution according to 4-8% of the volume of the fermentation substrate, and fermenting to obtain a schizophyllum commune fermentation system; a yeast fermentation stage: inoculating the kluyveromyces marxianus seed solution and the isatis orientalis seed solution into the schizophyllum commune fermentation system according to 2-4% of the volume of the fermentation substrate, and fermenting to obtain a yeast fermentation system; a bacterial fermentation stage: inoculating the acetobacter xylinum seed solution into a yeast fermentation system according to 4-8% of the volume of the fermentation substrate, and fermenting to obtain a mixed fermentation system; and post-treatment: carrying out inactivation, high-pressure homogenization and solid-liquid separation on the mixed fermentation system, and taking the liquid for sterilization and corrosion prevention to obtain the tea fermentation liquid. And cross-genus synergistic interaction is realized by a specific strain combination and a segmented culture process, and the multi-dimensional effect of a fermentation product is improved.

The invention relates to the technical field of wine brewing, and particularly discloses a microbial liquid for improving the quality of Maotai-flavor liquor and application thereof.Through the synergistic effect of three microbial agents, a continuous metabolic complementary network is formed in a brewing system, and clostridium tyrobutyricum provides organic acid and part of precursor substances in the anaerobic stage; the issatchenkia orientalis dominates alcoholic fermentation and synthesis of main esters, and creates a chemical environment beneficial to metabolism of other strains; the staphylococcus saprophyticus enhances protein conversion and generation of special flavor substances. According to the method, the metabolic pathways of acids, esters, pyrazines and phenols in a brewing system are enhanced, so that key flavor substances of the Maotai-flavor liquor are systematically improved, the fragrance of the liquor body is stronger and more harmonious, the taste is more mellow and full, and the overall quality is remarkably improved.

The invention relates to the technical field of deep processing of natural products, in particular to a fermentation preparation method of an active dandelion extract with functions of removing stasis and resisting inflammation, which comprises the following steps: crushing dried whole dandelion, and carrying out synergistic enzymolysis by using a compound enzyme consisting of cellulase, pectinase and beta-glucanase; then carrying out primary anaerobic fermentation of lactobacillus plantarum and secondary aerobic fermentation of issatchenkia orientalis in sequence; and finally, centrifuging, filtering, concentrating and spray-drying to obtain the high-activity dandelion fermentation extract. Through a process chain of ternary composite enzymolysis and step-by-step fermentation, an anaerobic-aerobic alternating micro-ecological system is constructed, and synergistic enrichment and functional quantification of double active components are realized. Specifically, in the final fermentation extract, the content of chicoric acid is greater than or equal to 8.5%, and the content of small molecular glycopeptide is greater than or equal to 25%, which are far higher than those of the traditional aqueous extract.

The invention discloses construction and application of an issatchenkia orientalis strain for producing succinic acid, and belongs to the technical field of microorganisms. Through a metabolic engineering means, a pyruvate decarboxylase coding gene on an issatchenkia orientalis genome is knocked out, and pyruvate carboxylase, malate dehydrogenase, fumarase and fumarate reductase are integrally expressed; a glycerol dehydrogenase coding gene on a genome is knocked out, dicarboxylic acid outer transport protein from schizosaccharomyces pombe is integrally expressed, carboxylic acid inner transport protein JEN2-1 and JENE2-2 on the genome are knocked out at the same time, and fumarate reductase and / or malic acid dehydrogenation are / is overexpressed. The succinic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 125.77 g / L, and the production intensity is 1.75 g / L / h. The engineering strain provided by the invention has good industrial application potential in efficient succinic acid production.

The invention discloses a combined fungicide and application thereof in clubroot resistance, and belongs to the technical field of microbial pesticides. The composite microbial agent comprises the following components in percentage by mass: 20%-30% of bacillus subtilis, 15%-25% of bacillus amyloliquefaciens, 10%-25% of lactobacillus mucus fermentation, 10%-20% of issatchenkia orientalis and 10%-20% of actinoplanes peritonei. All the strains have a synergistic effect, and the effect of preventing and treating clubroot is remarkable and is superior to that of a conventional drug chlorothalonil. By using the combined microbial agent provided by the invention to control clubroot, the usage amount of chemical pesticides can be reduced, and the combined microbial agent has the characteristics of environmental protection and high efficiency. Moreover, field trials show that after the combination fungicide is used for treatment, the clubroot morbidity of the cruciferae crops is reduced, meanwhile, the crop yield can be remarkably increased, and economic benefits are achieved. The combined microbial agent is suitable for actual production and is easy to popularize and apply.

The invention discloses construction and application of acid-resistant issatchenkia orientalis for producing D-lactic acid, and belongs to the technical field of bioengineering. By means of metabolic engineering transformation and lactic acid adaptive evolution, the acid-resistant issatchenkia orientalis capable of producing D-lactic acid at high yield under the low-pH condition is obtained, the yield of D-lactic acid in a 5 L fermentation tank can reach 150 g / L or above, the glucose conversion rate reaches 90% or above, the optical purity of D-lactic acid is larger than 99%, and the acid-resistant issatchenkia orientalis has good genetic stability and is suitable for industrial production. 10 acid-resistant genes are also screened and identified, so that the lactic acid fermentation capacity of the strain at low pH can be effectively enhanced.