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Biological detoxification method and detoxification active bacterial strain of hemicellulose hydrolysate

A hydrolyzate and semi-fiber technology, applied in the biological field, can solve the problems of cumbersome process and high cost

Inactive Publication Date: 2010-06-16
唐传生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the problems of cumbersome process and high cost in the existing hemicellulose hydrolysis physical chemical detoxification process

Method used

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  • Biological detoxification method and detoxification active bacterial strain of hemicellulose hydrolysate
  • Biological detoxification method and detoxification active bacterial strain of hemicellulose hydrolysate
  • Biological detoxification method and detoxification active bacterial strain of hemicellulose hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Weigh 3kg of bagasse that has been washed and re-dried, add H2SO4 solution (2.5%, w / w) according to the solid-to-liquid ratio (w / v) of 1:7, hydrolyze for 2h at 120°C, collect the filtrate by centrifugation, wash the filter residue once, and combine The filtrate was adjusted to pH 3 with solid calcium carbonate, and the precipitate was removed by suction filtration to obtain bagasse hemicellulose hydrolyzate. The reducing sugar content of the hydrolyzate is 3%, and xylose monosaccharide accounts for more than 80% of the total sugar.

Embodiment 2

[0082] Take 2 kg of crushed dry corncobs, add 2% (w / w) H at a solid-to-liquid ratio of 1:7 2 SO 4 The solution was hydrolyzed at 120°C for 2 hours, the liquid part was collected, neutralized with calcium carbonate to a pH value of 3, and the precipitate was removed by suction filtration to obtain a dilute acid hydrolyzate of corncob hemicellulose. The total sugar content of the hydrolyzate is 5%, wherein xylose accounts for about 60% of the total sugar.

Embodiment 3

[0084] Inoculate the slant of I. orientalis CCTCC NO: M206098 on the liquid seed medium, culture at 200rmp, 30°C on a shaker for 12h, and obtain the activated seed liquid. Insert the inoculum amount of 15% (v / v) into Example 1, in the dilute acid hydrolyzate of bagasse hemicellulose obtained, 200rmp, 33 ℃ of shaking table fermentation detoxification 45h, centrifuge supernatant is the product obtained by Oriental Yisa Yeast CCTCC NO: M206098 Bio-detoxified bagasse hemihydrolyzate.

[0085] Under the same conditions, the bagasse hemicellulose hydrolyzate in Example 1 was treated with I. westernis CCTCC NO: M206097 to obtain the bagasse hemicellulose hydrolyzate biodetoxified by I. westernia CCTCC NO: M206097.

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Abstract

The invention discloses a method for degrading and removing microbial metabolism inhibitors existing in a hemicellulose diluted acid hydrolysate by using a detoxification active bacterial strain. The detoxification active bacterial strain comprises issatchenkia orientalis CCTCC NO:M 206098 and issatchenkia occidentalis CCTCC NO:M 203097, and both the ssatchenkia orientalis and the issatchenkia occidentalis can degrade the microbial metabolism inhibitors which comprise acetic acid, furfural and phenols substances and exist in the hemicellulose hydrolysate and have the characteristics of low pH value resistance and no utilization of pentose or sugar alcohol thereof. The detoxification active bacterial strain can be used for carrying out single bacterial detoxification treatment on the hemicellulose hydrolysate and can also generate xylitol or alcohol with fermented xylose for bacterial strain mixing and inoculating to the hemicellulose hydrolysate to carry out synchronous detoxification and fermentation. The biological detoxification method can effectively improve the fermenting property of the hemicellulose hydrolysate and improve the yield of fermentation products of the alcohol and the xylitol and has the advantages of environmental protection and energy saving.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a biological detoxification method of hemifiber hydrolyzate and a special detoxification active microbial strain. Background technique [0002] Hemicellulose is a polysaccharide other than cellulose in the plant cell wall. The content varies with different plants, accounting for 20-35% of the weight of the plant. It is the most abundant polysaccharide on the earth except cellulose. [0003] Cellulose is a homogeneous polysaccharide composed entirely of glucose units, while hemicellulose is mainly composed of xylose with β-(1-4) glycosidic bonds to form the main chain, and arabinose, mannose and galactose form branches. Non-uniform glycans with a chain structure. The xylan backbone of hemicellulose generally contains 50-150 xylose units, has no crystalline structure, and is bound to the surface of cellulose microfibrils. [0004] Hemicellulose is much easier to hydrolyze than ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P39/00C12P19/02C12P7/06C12R1/74C12R1/645
CPCY02E50/17Y02E50/10
Inventor 张厚瑞覃香香蔡爱华周玉恒陈海珊
Owner 唐传生物科技(厦门)有限公司
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