358 results about "Microbial metabolism" patented technology

The invention provides a microbe-enzyme composite preparation used for restoring water in an urban and rural polluted river, and its preparation method. The urban and rural polluted river restoration microbe-enzyme composite preparation is characterized in that the microbe mainly comprises 30-60% of Bacillus licheniformis, 25-35% of a nitrobacterium, and 15-35% of pseudomonas putida; and the composite enzyme mainly comprises 40-60% of a protease, 15-25% of an amylase and 25-35% of a cellulase. The optimized microbe is compounded with the composite enzyme to prepare the microbe-enzyme composite preparation against water of different pollution degrees, the addition amount of the microbe is 1-5g/m<3>, and the addition amount of the composite enzyme is 0.01-0.05g/m<3>. The microbe-enzyme composite preparation is added to the urban and rural polluted river to carry out microbial reinforced restoration with aeration as an auxiliary measure, can effectively improve defects of low microbe metabolism activity, low environmental tolerance, short retention time in the polluted water and the like existing in the prior art, and has a substantial water purifying effect.

A method and apparatus in which in a biological treating system for organic wastewater, not only is phosphorus, etc. efficiently recovered as MAP crystal from a wastewater containing organic matter, phosphorus and nitrogen but also the biological denitrification/dephosphorization treatment capability is strikingly improved. There is provided a method of treating sludge generated in the process for biological treatment of organic wastewater utilizing microbial metabolism, characterized in that a biological treatment composed mainly of acid fermentation by facultative anaerobes is carried out with the sludge to thereby leach phosphate ion and ammonium ion from the sludge; a magnesium source is added to the sludge to thereby form grains of magnesium ammonium phosphate from the leached phosphate ion and ammonium ion; and grains of magnesium ammonium phosphate are separated from the sludge containing formed grains of magnesium ammonium phosphate. Further, there is provided an apparatus for carrying out the method.

The invention relates to a method for preparing bactericide by using derivative bacterial of growth promoting antagonist bacterial M18, preparing fermentation liquor with high concentration of under phenazine-1-carboxyl acid and pyoluteorin under optimized culture medium and condition by using derivative bacterial M18G and M18R of growth promoting antagonist bacterial M18, preparing the fermentation liquor M18G and M18R into dry powder, double crossing physically the phenazine-1-carboxyl acid in M18G powder and pyoluteorin in M18R according to the weight ratio of 90%-10% and 10%-90%, and finally getting bactericide of high efficiency for preventing plant disease. Compared with current technology, the bactericide provided in this invention is wet powder taking metabolite of microorgsanism as active element but not live bacterial agent, as a result of which the product is characterized by high stability, not easy to be influenced by environment and better prevention and curing effect for multiple plant disease under low consumption.

The invention relates to the technical field of microbe application, specifically relates to a microbial agent and application thereof to saline-alkali land soil improvement. The microbial agent is prepared by compounding bacillus amyloliquefaciens Bn-21, Bacillus subtilis Bn-3,Bacillus pumilus Bn-19, Rhodopseudomonas palustris Bn-2 and lactobacillus plantarum Bn-23, the compounded multiple microbes has different functions, each microbe carries out different microbe metabolic activity, every two bacterial strains have no antagonism, the function of each microbe is relatively stable, can play a different role in a soil conditioner, increase microbe diversity in soil, enhance activity of the microbe in soil, strengthen the effects of the soil conditioner for improving soil physical and chemical properties and improving the soil fertility.

The invention discloses a composite bacterial agent and a biological method for treating flow-back fracturing fluid to obtain oil displacement active water, which relate to biotransformation and reutilization technology of fracturing flow-back fluid. In the method, the fracturing flow-back fluid is treated by a microbial fermentation method, and a residual organic substance harmful to environment in the flow-back fluid is transformed into biological active water with certain function. The composite bacterial agent and the method are applied to reinjection water of water injection wells, reinjection water for single-well soaking, reinjection water for oil field blockage removal, reinjection water for other purposes, water for medicament preparation in a technical process and the like. The composite bacterial agent comprises biosurfactant, organic acid, organic ester, organic ketone, biogas and other metabolites produced by microbial metabolism of the fracturing flow-back fluid.

The invention relates to a bacillus licheniformis engineering bacterium for efficiently synthetizing poly-gamma-glutamic acid, and belongs to the field of microbial metabolism engineering. A conventional molecular biological technique is used; on the basis of bacillus licheniformis WX-02 stored in a laboratory, a double-ingredient regulation and control factor degU is expressed in an enhanced way through external sources; the bacillus licheniformis engineering bacterium WX-02/pHY-degU capable of efficiently synthetizing the poly-gamma-glutamic acid under the condition of no addition of an external source of glutamic acid is obtained. Liquid fermentation proves that the concentration of the poly-gamma-glutamic acid at a fermentation end point reaches 32.78 g/L; compared with that of an original bacterial strain of bacillus licheniformis WX 02, the concentration is improved by 47.59 percent; the synthesis capability of the poly-gamma-glutamic acid is obviously enhanced. The bacterial strain is preserved in CCTCC (China Center For Type Culture Collection) on May 27, 2016, and the preservation number is CCTCC NO:M2016294.

The invention provides a movable and combined subsurface constructed wetland treatment device and method. The device comprises an outer net, an inner net, a separating and filtering net, a ring buckle and a filler layer, wherein the outer net is arranged on the outermost side; the inner net is closely attached to the outer net; the separating and filtering net is closely attached to the inner net and filled with filler; the ring buckle is arranged in the center of the top of the outer net; and plants are planted on the surface of the filler layer. The devices can be connected in parallel to form a single treatment pool system, and multiple treatment pools can be connected in series to form a multi-treatment-pool system; the devices can also be produced in batches in advance, plants are planted, microorganisms are tamed, and the devices can be installed immediately and work immediately when necessary. The system performs purification treatment on sewage through multiple effects such as sedimentation, filtration, chemical precipitation, adsorption, decomposition, microbial metabolism, plant metabolism, plant root absorption and the like, has the advantages that the system is not limited by sites, capable of being produced in a modularized manner and operated in a combined manner, reproducible, removable, replaceable, convenient to manage and maintain, low in cost and the like, and is broad in application prospect.

The invention discloses a sludge reflow-free device with a simultaneous nitrification and denitrification (SND) function. The device comprises a pool body; the sidewall of the pool body is provided with a main water inlet; the interior of the pool body is divided by a clapboard into a diversion area, an aeration area and a sludge precipitation area in sequence; the aeration area is internally provided with an overflow groove; the bottom of the aeration area is provided with microporous aeration devices distributed uniformly; and the sludge precipitation area is internally provided with a membrane element. The invention also discloses an operation control method of the device. By skillful arrangement of aeration pipes and combined use with a biological membrane, the power cost and the operating cost of sludge reflow are saved, and the purpose of reducing energy consumption is achieved. The biological environment to be achieved by the simultaneous nitrification and denitrification is adjusted indirectly through adjusting parameters such as active sludge concentration, sludge age, unit sludge load and the like; the operation is strong in human controllability, and the problem that the microbial metabolic environment in the SND technology is not easy to control is solved.

The invention relates to recombinant escherichia coli for producing 2'-fucosyllactose and application thereof, and belongs to the field of microbial metabolic engineering. According to a regulation and control strategy for the expression level of 2'-fucosyllactose de novo synthesis route key enzyme, different enzymes are subjected to regulation and control of genome overexpression or plasmid overexpression, the efficiency of a metabolic pathway can be greatly improved, compared with the prior art, the efficiency of a genetic engineering strain constructed through related strategies is improvedby 13 times or above, and a foundation is laid for industrial production of the 2'-fucosyllactose.

The invention discloses a novel environment-friendly feed additive and a preparation method thereof. The additive mainly comprises a compound Chinese medicinal preparation, germ bran of Cordyceps militaris, and compound probiotics. Various probiotics are added for mixed multi-stage fermentation, aerobic fermentation first and then anaerobic fermentation are performed, rich organic acid and biological enzymes are produced in the microbial metabolism process, plant cell walls are broken, active ingredients are fully released, and the absorption and utilization rate is improved, so that the therapeutic effect of Chinese medicines is improved. In the method, three nationally permitted environment-friendly feed additives, namely the probiotics, an enzyme preparation and the compound Chinese medicinal herb preparation are mixed, and the effects of the probiotics, the enzyme preparation and the compound Chinese medicinal herb preparation are organically combined after two fermentation treatments, are complementary, and are not simply mixed; the effect of the additive is greatly improved than those of environment-friendly feed additives sold on the market at present, but the cost is almost not increased; and the source of raw materials is wide, the cost is low, and the additive is favorable for large-scale market promotion.

The invention relates to a treatment method of anaerobic fermentation biogas slurry from livestock and poultry raising. After pH adjustment, the anaerobic fermentation biogas slurry enters an ammonia blow-off column for ammonia nitrogen pretreatment; after pH adjustment, the effluent enters a SBR pool for biochemical treatment; easily-degraded COD is removed through microbial metabolism in the SBR pool; ammonia and nitrogen are further removed through nitrification and denitrification; and total phosphorus is also removed partially; SBR effluent enters a coagulation slanting board sedimentation pool to complete removal of difficultly-degraded organic matter and residual total phosphorus. The invention provides simple, high-efficient, highly-applicable, and standard treatment technology ofanaerobic fermentation biogas slurry from livestock and poultry raising.

A Strain producing dynamic controlling recombinant strain and a method for preparing D-lactic acid with the recombinant strain belong to the technical field of genetically engineered agricultural microorganisms. The recombinant strain is named (Escherichia coli)B0013-070B, and is preserved in the China center for type culture collection, and the preservation number is CCTCCNO:M2012071. A lactate dehydrogenase gene promoter ldhAp in the genome of the strain is replaced with a culture environment/nutritional factors control-type promoter. Through the utilization of the recombinant strain, fermentation is conducted for 28 to 40 hours in stages at 25 to 50 DEG C, and the level of producing D-lactic acid reaches 12.5%; the optical purity lives up to 99.9%; and the chemical purity reaches to 98.4%. The dynamic controlling to the D-lactic acid dehydrogenase encoding gene expression on D-lactic acid high-producing strain B0013-070 chromosomes is conducted, so that the gene expression is controlled only through changing the fermentation temperature during the D-lactic acid production process to achieve the purpose of efficiently synthesizing D-lactic acid through taking glucose as a raw material. After simple modification, the invention can be used for producing important microbial metabolites in other industries.

The invention discloses a combined aerobic and anaerobic microorganism treatment system and method for gas in a mine goaf. The system comprises two parts: an aerobic methane-oxidizing bacteria agent spraying system and an anaerobic methane-oxidizing bacteria agent spraying system; the components comprise a methane-oxidizing bacteria agent storage tank, a pressure pump, a spraying pipe network, a nozzle and the like; the methane-oxidizing bacteria agent storage tank is connected with the input end of the pressure pump, and the output end of the pressure pump is connected with the spraying pipenetwork; the spraying pipe network is connected with the nozzle to form the spraying system. The method comprises the following steps: (1) culturing aerobic and anaerobic methane-oxidizing bacteria agents; (2) arranging the aerobic methane-oxidizing bacteria agent spraying pipe network system; (3) arranging the anaerobic methane-oxidizing bacteria agent spraying pipe network system; (4) regularlyspraying a methane-oxidizing bacteria agent and a culture solution; (5) testing effects. The danger of gas in the goaf can be reduced by regularly spraying the methane-oxidizing bacteria agent and theculture solution thereof into the goaf and removing methane by utilizing microbial metabolism.

The invention discloses a winter temperature maintaining and raising device for a constructed wetland. The main structure of the device is that: three sections of fillers are vertically and parallelly filled in the constructed wetland, the tops of the three sections of fillers are provided with a water distribution pipe, the bottoms of the three sections of fillers are provided with a water receiving pipe, and a water outlet of the water receiving pipe and the tops of the fillers are at the same height; the upper part of one end of the three sections of fillers is connected with a water inletpipe, the lower part of the other end of the three sections of fillers is connected with a water outlet pipe, and a water outlet of the water outlet pipe and the tops of the fillers are at the same height; the water distribution pipe and the water inlet pipe are connected to a high-position water tank respectively; when water flows into the water distribution pipe, the water is discharged from the water outlet of the water receiving pipe, and the water inlet pipe and the water outlet pipe are closed; and when the water flows into the water inlet pipe, the water is discharged from the water outlet of the water outlet pipe, and the water distribution pipe and the water receiving pipe are closed. A guardrail at the periphery of the constructed wetland is double walls, and organic matter for fermenting to produce heat is filled inside the double walls. The temperature of the wetland is maintained and raised by the heat produced by microbial metabolism in a double wall structure; and the disadvantages that the content of dissolved oxygen of influent of a horizontal flow wetland is low in summer and a vertical flow wetland is high in heat loss in winter are overcome.

The invention discloses a bacillus cereus for activating soil nutrient and application of the bacillus cereus. The bacillus cereus is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 21, 2012 with preservation number of CGMCC No. 6127, and the 16SrDNA nucleotide sequence is shown by SEQ ID No: 1. According to the bacillus cereus, the soil nutrient can be obviously activated, the effectiveness of phosphorus and nitrogen in soil can be improved, the composition of a soil microbial community can be improved, the diversity and the evenness of the soil microbial community can be improved, the metabolic activity of the soil microbe can be improved and the like, the soil fertility can be improved, the crop growth can be promoted, the bacillus cereus is good for the agricultural sustainable development and utilization, and the bacillus cereus is wide in application prospect in the production practice.

The invention relates to a method for production of at least one non-volatile microbial metabolism product in solid form by sugar-based microbial fermentation, wherein a microbial strain producing the required metabolism product is cultivated with a sugary liquid medium with a monosaccharide content of more than 20 wt. %, based on the total weight of the liquid medium, the volatile components of the fermentation brew are mostly removed, the sugary liquid medium being produced by: a1) milling a starch source selected from cereal grains, a2) liquefying the milled material in an aqueous liquid in the presence of at least one starch-digesting enzyme and subsequent saccharification using at least one saccharifying enzyme, wherein the liquefying is carried out with addition of a partial amount of the milled material to the aqueous fluid continuously or batch-wise. The invention further relates to a solid formulation obtained by the above method of a non-volatile microbial metabolism product and the use of such a solid formulation as additive or supplement to animal or human food or for textile, leather, cellulose, paper or surface treatments.

The invention discloses an apparatus and a method for preparing adiponitrile by electrolyzing acrylonitrile assisted by electro-active microbes. The apparatus comprises a microbe electrolytic tank, a data acquisition system and a recording unit, wherein an anode electrode and a cathode electrode of the microbe electrolytic tank are made of electrically inert materials; the anode electrode and the cathode electrode are connected through a titanium wire, a constant potential rectifier and a resistor; the data acquisition system is connected with the resistor in parallel; and the recording unit is connected with the data acquisition system. The method comprises the steps of organic materials are metabolized by electro-active microbes in an anode chamber of the microbe electrolytic tank to produce electrons and protons; the produced electrons are transferred to the anode electrode directly or indirectly and then transferred to the cathode electrode along an external circuit under the effect of an applied voltage; at the same time, H<+> ions pass through an ion exchange membrane and are migrated to a cathode chamber of the microbe electrolytic tank; H<+> ions, the electrons and acrylonitrile are combined on the surface of the cathode electrode with relatively high hydrogen evolution over potential to generate adiponitrile. The apparatus and the method have the advantages of low energy consumption, no corrosions to the anode electrode, etc. Besides, the apparatus and the method can reduce discharge of organic wastes.

A method for improving petroleum recovery efficiency through air foam flooding assisted by microorganisms comprises the steps of composition of a microorganism-assisted air foam system and injection of the microorganism-assisted air foam system, wherein the microorganism-assisted air foam system is composed of microorganism nutrients, microorganism fermentation liquor and an air foam system; according to the step of injection of the microorganism-assisted air foam system, before construction, the oxygen-consuming microorganism fermentation liquor is injected into an immediate vicinity of wellbore of an injection well, and the concentration of native aerobic bacteria in crude oil of the immediate vicinity of wellbore of the injection well is improved; then the air foam system with the microorganism nutrients is injected into an oil layer, microorganisms quickly ferment through the nutrients and air so as to consume oxygen in air, and the content of oxygen is reduced to be smaller than a safety value. By means of the method for improving recovery efficiency through air foam flooding assisted by microorganisms, the content of oxygen in air of the injection well can be lowered to be lower than 3%, and oxygen detected in an oil well is nearly zero; meanwhile, microbial metabolism surface active agents improve the performance of air foam and the crude oil recovery efficiency to some extent.

The invention relates to an enhanced phosphorous undercurrent removing artificial wetland for treating high-phosphorus sewage, which comprises a bed body, a mixed packing layer, a mixed soil layer, a water distribution tank, a water inlet perforation plate, a water outlet perforation plate and a water collecting basin. When the artificial wetland is in work, the sewage enters the water distribution tank and flows into the mixed packing layer through the water inlet perforation plate, then the sewage soaks towards the mixed soil layer slowly, and under the adsorption sedimentation actions of the packing layer and the soil layer and through microbial metabolism, the pollutants such as organic substances, nitrogen and phosphorus and the like in the sewage are removed. Simultaneously, aquatic plants can absorb part of the nitrogen and the phosphorus as nutrition substances supplied to per se metabolism so that advanced treatment is performed on the sewage. The artificial land has the characteristics of simplicity, practicability, high hydraulic load, good nitrogen and phosphorus treatment effect, stable performance, low running cost and the like.