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374 results about "Microbial metabolism" patented technology

Microbial metabolism is the means by which a microbe obtains the energy and nutrients (e.g. carbon) it needs to live and reproduce. Microbes use many different types of metabolic strategies and species can often be differentiated from each other based on metabolic characteristics. The specific metabolic properties of a microbe are the major factors in determining that microbe's ecological niche, and often allow for that microbe to be useful in industrial processes or responsible for biogeochemical cycles.

Sewage treatment control process realized based on dynamic regulation and control of aeration and carbon source addition

The invention relates to the technical field of sewage treatment, in particular to a sewage treatment control process based on dynamic regulation and control of aeration and carbon source addition, which comprises the following steps: acquiring microbial metabolism heat change data in real time; biochemical treatment: monitoring the dielectric constant of a water body and the Reynolds number of sewage flow in real time, and constructing a multi-parameter coupling regulation and control model and dynamically regulating and controlling the aeration rate in combination with microbial metabolism heat change data obtained in the pretreatment step; accurately adding the carbon source according to a calculation result of the carbon source demand prediction model; sewage subjected to biochemical treatment is subjected to ultrafiltration through a nanofiber membrane and then subjected to combined disinfection treatment through ultraviolet rays and ozone, treatment parameters are regulated and controlled in real time according to the membrane flux attenuation trend in the treatment process, and dynamic and accurate control over sewage treatment is achieved. According to the method, the problem of energy consumption waste caused by excessive aeration or low degradation efficiency caused by insufficient aeration in a traditional process is solved, and accurate matching of dissolved oxygen supply and microbial requirements is realized.
Owner:SHANDONG XIANGMING SHUZHI IOT TECH CO LTD

Intelligent regulation and control method for tea fermentation process and regulation and control system based on microbial activity monitoring

The invention discloses an intelligent regulation and control method for a tea fermentation process and a regulation and control system based on microbial activity monitoring, and relates to the technical field of tea processing. According to the method, microbial activity and environmental parameters are monitored in real time, and a microbial metabolism model and a neural network algorithm are combined, so that dynamic optimization and accurate regulation and control of the tea leaf fermentation process are realized, firstly, microbial activity data and environmental parameters in a fermentation environment are collected by utilizing a sensor array; the method comprises the following steps: firstly, calculating a metabolite generation rate and a concentration change trend through a microbial metabolism model, accurately identifying abnormal fluctuation in a fermentation process, secondly, adjusting fermentation conditions in real time based on the metabolite concentration change trend and a dynamic regulation and control algorithm to ensure the stability of the fermentation process, and finally, carrying out real-time fermentation. Tea quality parameters after fermentation condition adjustment are predicted through a neural network algorithm, a regulation and control strategy is optimized, and stable improvement of tea quality is achieved.
Owner:WANGCANG GAOYANG BIFENG TEA CO LTD

Large-scale pig breeding wastewater purification treatment system

The invention discloses a large-scale pig breeding wastewater purification treatment system, and relates to the technical field of wastewater treatment.The large-scale pig breeding wastewater purification treatment system is characterized in that the ion concentration of anaerobic liquid is monitored through a multi-ion sensing array, and a multi-channel feeding system is controlled to synthesize customized struvite compound fertilizer; the AI prediction control unit predicts the optimal medicament dosage and realizes millisecond-level closed-loop regulation; the multi-dimensional synergistic biochemical treatment module comprises an A / O biochemical treatment unit and an electric field enhanced anaerobic ammonia oxidation reactor, the electric field enhanced anaerobic ammonia oxidation reactor applies a direct-current electric field through an electrode pair, and engineering conductive biochar is used as a carrier to accelerate microbial metabolism; the sludge low-temperature pyrolysis self-consistent circulation module is used for pyrolyzing and converting residual sludge into biochar, biofuel and wood vinegar, and the biochar activation treatment unit is used for preparing engineering conductive biochar and forming a substance closed loop through a self-consistent circulation conveying system; and the digital twinning intelligent management and control platform realizes intelligent management and control of the system.
Owner:YUNNAN BINGSEN ENVIRONMENT ENG

Wastewater treatment equipment remote control system based on Internet of Things

The invention belongs to the technical field of wastewater treatment, and provides a wastewater treatment equipment remote control system based on the Internet of Things, which aims at solving the problems that the traditional fixed DO concentration control cannot adapt to water inlet load fluctuation, the effluent is easy to be substandard or the aeration energy consumption is high, the monitoring data noise is large, and the time sequence is misplaced. According to the scheme, a multi-dimensional real-time sensing network is constructed, flow, COD, BOD, ammonia nitrogen, water temperature, distributed DO and sludge activity sensors are deployed, discrete wavelet transform is adopted for noise reduction, and a data time sequence is aligned; a three-layer load-DO-energy consumption dynamic correlation model is designed, a basic layer predicts a load trend through LSTM, a middle layer quantifies a DO demand through a microbial metabolism model, and an optimization layer outputs an optimal DO set value through a PPO algorithm; and developing a dynamic decision-making system, adaptively generating a DO interval according to a load state, and adjusting fan parameters and number in a linkage manner. According to the invention, load dynamic adaptation is realized, effluent ammonia nitrogen is guaranteed to reach the standard, aeration energy consumption is reduced, and remote control precision and equipment operation efficiency are improved.
Owner:JIANGXI YUANXIN RESOURCE RECYCLING INVESTMENT DEV

Control system for sewage treatment plant

The invention relates to the technical field of sewage treatment automatic control, and discloses a control system for a sewage treatment plant, which comprises a multi-modal biosensing module, the multi-modal biosensing module is deployed in an anaerobic zone and an aerobic zone of a biochemical reaction tank, and a cloud edge collaborative data processing module comprises an edge calculation module and a cloud optimization platform. The edge calculation module preprocesses data collected by the multi-mode biosensing module and executes emergency control, the cloud optimization platform operates the multi-target dynamic optimization module and the digital twin model, and the self-adaptive decoupling controller receives control parameters output by the multi-target dynamic optimization module. By deploying the multi-modal biosensing modules in the anaerobic zone and the aerobic zone, the real-time acquisition of biochemical reaction parameters of each zone along the way is realized, and the microbial metabolic activity change in the biological reaction process can be accurately captured in combination with the pretreatment of the edge calculation module and the analysis of the cloud optimization platform.
Owner:JIANGSU FUWEI ELECTRIC AUTOMATION CO LTD

Fe / C load-based membrane module, PN / A-MABR autotrophic nitrogen removal device, preparation method and sewage treatment method

The invention discloses a Fe / C loaded membrane module, a PN / A-MABR autotrophic nitrogen removal device, a preparation method and a sewage treatment method, and belongs to the technical field of sewage treatment. According to the invention, the problem that the treatment effect is influenced by NO3 <->-N accumulation in the existing membrane aeration coupling partial nitrification-anaerobic ammonia oxidation process is solved. Comprising a frame and a membrane body attached to the outer portion of the frame, the membrane body comprises a hydrophobic organic membrane layer, an iron-carbon material layer and a biological membrane layer which are sequentially arranged, and the hydrophobic organic membrane layer is attached to the outer portion of the frame. The iron-carbon material is added between the hydrophobic organic membrane layer and the biological membrane layer, the iron-carbon micro-electrolysis process is coupled with the dissimilatory process of nitrate generated by microbial metabolism, the removal of NO3 <->-N generated by anaerobic ammonia oxidation is enhanced, and the total nitrogen removal efficiency is improved.
Owner:HARBIN INST OF TECH

Nitrogen-oxygen co-doped hypha / spruce composite adsorption material and copper-containing sewage treatment method

The invention belongs to the technical field of water treatment, and particularly provides a nitrogen-oxygen co-doped hypha / spruce composite adsorption material and a copper-containing sewage treatment method. According to the invention, the copper-containing sewage is pretreated by using the solid fermentation product, and then the copper-containing sewage is post-treated by using the nitrogen-oxygen co-doped hypha / spruce composite adsorption material prepared under specific conditions; wherein in the pretreatment stage, microbial metabolites in a solid fermentation product preliminarily capture free copper ions (Cu < 2 + >) through complexation, the toxicity of the sewage is reduced, and meanwhile, the copper ions are partially converted into a form which is easier to adsorb. In the post-treatment stage, the nitrogen-oxygen co-doped hypha / spruce composite adsorption material plays a key role, and efficient interception and adsorption of copper ions are achieved. In conclusion, the treatment method for the copper-containing sewage comprises a pretreatment stage and a post-treatment stage, the two stages are in synergistic interaction, and finally the removal rate of the heavy metal copper can be effectively increased.
Owner:BEIHUA UNIV +1

Method for measuring carbon utilization efficiency of soil microorganisms

ActiveCN120741819AEarth material testingProteomicsIsotopic labelingMicrobial respiration
The invention discloses a soil microorganism carbon utilization efficiency determination method, and relates to the technical field of carbon utilization efficiency determination, and the method comprises soil sample pre-culture, multi-source 18O isotope gradient labeling, dynamic gas monitoring, microorganism respiration rate dynamic correction, and microorganism growth rate and carbon utilization efficiency acquisition. By labeling multi-source isotopes and quantifying contributions of different oxygen sources to microorganism DNA synthesis, underestimation of the carbon utilization efficiency caused by a traditional single labeling method is avoided, the problem of limitation existing in the current soil microorganism carbon utilization efficiency determination process is solved, the inhibition effect of too high local concentration on microorganism metabolism is reduced through gradient labeling, and the accuracy of soil microorganism carbon utilization efficiency determination is improved. The method has the advantages that marking uniformity is improved, multiple groups of soil samples are arranged, real-time change of microbial respiration is captured by means of dynamic monitoring, influence of isotope fractionation on carbon dioxide concentration calculation is eliminated, instantaneous errors of traditional static measurement are avoided, and accuracy of analysis results is improved to a certain extent.
Owner:SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI

Preparation method of composite slow-release carbon source

The invention discloses a preparation method of a composite slow-release carbon source, which comprises the following steps: preparing an inner core: uniformly dry-mixing lignin powder and a quick-acting carbon source, and adding a first binder to prepare inner core particles; middle layer wrapping: alternately spraying a second binder and middle layer coating powder until the middle layer is completely wrapped to obtain double-layer particles; outer layer wrapping: alternately spraying a third binder and outer layer coating powder until the outer layer is completely wrapped to obtain three layers of particles; and spraying a biodegradable polymer solution on the surfaces of the three layers of particles to form a coating film. Through the three-layer structural design of the quick start layer, the stable maintenance layer and the long-acting guarantee layer, accurate matching of carbon source release and microbial metabolism requirements is realized. The outer-layer quick-acting carbon source quickly starts microbial activity, the middle layer provides stable and continuous carbon source supply, and the inner core ensures the tail-section treatment effect. The sequential release mechanism effectively avoids burst release waste of the carbon source, so that the utilization rate of the carbon source is greatly improved.
Owner:JURONG DEEPWATER WATER CO LTD +2

A Fe-GC@Fe 0 Catalysts, their preparation methods and applications

This invention discloses a Fe-GC@Fe 0 This invention relates to catalysts, their preparation methods, and applications, belonging to the field of water treatment technology. The preparation method of this invention is simple, requires minimal equipment, and yields Fe-GC@Fe catalysts. 0 The catalyst can drive microorganisms in water to metabolize perfluorinated compounds. Under conditions without external energy input, it can effectively remove perfluorinated compounds such as perfluorooctanoic acid, perfluorooctane sulfonic acid, perfluorononanoic acid, and perfluorohexane sulfonic acid from water. The removal rate and defluorination rate both reach more than 80%. Moreover, it is a solid catalyst, which is easy to separate from water, recycle and reuse, and maintain a high removal effect.
Owner:GUANGZHOU UNIVERSITY

Central air conditioner pollution degree autonomous evaluation robot based on multi-mode perception

The invention discloses a central air conditioner pollution degree autonomous evaluation robot based on multi-modal perception, and relates to the technical field of intelligent detection, the robot comprises a voxel space division module, a data acquisition module, a pollution characteristic mapping modeling module, a physical pollution detection module, a chemical pollution detection module and a biological pollution detection module; through three-dimensional point cloud data scanning and real-time data acquisition, the air pipe wall surface roughness, the temperature delay time, the gas fluctuation, the heat retention degree and the microbial metabolism gas concentration are monitored in real time, and a three-dimensional pollution characteristic mapping model is generated according to the pollution characteristic data set; by calculating a physical pollution index PPI, a chemical pollution index CPI and a biological pollution index BPI and comparing and analyzing with corresponding threshold values, whether dust retention, gas concentration and microorganism quantity in an air pipe exceed standards or not is judged in real time, cleaning, filtering and sterilizing strategies are automatically triggered, and the air quality and operation safety of a central air conditioning system are effectively guaranteed.
Owner:GUANGZHOU QINGXIN ENVIRONMENTAL PROTECTION TECH CO LTD

Method for increasing yield of 2 '-fucosyllactose through synergistic effect of multiple alpha-1, 2-fucosyltransferases

The invention relates to the field of microbial metabolism engineering, and discloses a method for increasing the yield of 2 '-fucosyllactose (2'-FL) through the synergistic effect of various alpha-1, 2-fucosyltransferases. According to the method, two or more alpha-1, 2-fucosyltransferases with different sources are co-expressed in microbial cells, and the complementary enzymatic characteristics of the alpha-1, 2-fucosyltransferases are utilized, so that the synthesis efficiency of the 2 '-FL is remarkably improved, and the yield of the 2'-FL is effectively improved. The microbial cell is escherichia coli C43 (DE3) (E. coli C43 lacZ waaF with lacZ and waaF genes knocked out, the serial number is SL), a pETDuet-1 plasmid is constructed, and genes manB, manC, gmd, wcaG and zwf are overexpressed on a pETDuet-1 vector, so that the microbial cell can be used for preparing the microbial cell. In a 5L fermentation tank, after the final strain SL07 is used for final fermentation for 68 hours, the yield of 2 '-FL reaches 148g / L, and the production intensity reaches 2.18 g / L / h. The embodiment shows that the engineering strain for co-expressing the alpha-1, 2-fucosyltransferase from different sources can obviously improve the yield of the 2 '-FL.
Owner:BEIJING ZENUO TECH DEV CO LTD

Method and system for analyzing bacterium-drug interaction panoramic dynamic mechanism by using causal enlightenment hypergraph neural network, and electronic equipment

The invention discloses a method and system for analyzing a bacterium-drug interaction panoramic dynamic mechanism by using a causal enlightenment hypergraph neural network, and electronic equipment, and belongs to the technical field of artificial intelligence and biomedical informatics. The method comprises the following steps: constructing a disease / syndrome / disease-bacterium / microorganism / metabolite-prescription / drug / component interlocking hypergraph; analyzing a bacteria-drug interaction dynamic mechanism based on a dynamic hypergraph network algorithm; optimizing the hypergraph neural network based on the prescription compatibility rule and the traditional Chinese medicine dosage; and predicting the microbial marker based on the causal enlightenment hypergraph neural network. According to the method, a multi-entity high-order relation is captured through an interlocking hypergraph structure, a time sliding window and an attenuation mechanism are introduced to analyze dynamic evolution, the traditional Chinese medicine dosage is used as an attention priori optimization network, and causal reasoning is achieved through alternate optimization of an intervention discovery and response prediction module. In a public data set test, the performance evaluation index is obviously superior to that in the prior art, and the problem of analyzing the panoramic dynamic mechanism of the bacterium-drug interaction is effectively solved.
Owner:HUNAN ACAD OF CHINESE MEDICINE

Functional composite suspended biological carrier and preparation and application methods thereof

The invention provides a functional composite suspended biological carrier and a preparation and application method thereof. The functional composite suspended biological carrier comprises a main base material and functional components, the main body base material is polyethylene or polypropylene, and the functional component comprises the following substances in parts by mass: 3.0 parts of polyquaternium-10 or cationic polyacrylamide, 1.5 parts of molybdenum disulfide, 2.5 parts of vinyl acetate or acrylate, 2.0 parts of nano silicon dioxide, 2-3 parts of talcum powder, 0.5 part of a silane coupling agent and 0.5 part of polyethylene wax; the total proportion of inorganic components in the carrier is less than or equal to 12%; the cross section of the carrier is a 19-hole honeycomb hollow cylinder, the diameter of the formed cylinder is 25 mm, the height of the formed cylinder is 10 mm, and the density of the carrier is 0.95-1.00 g / cm; according to the invention, the state of charge on the surface of the carrier is adjusted through the positive electricity polymer PQAS-10, the affinity with negatively charged microorganisms is enhanced, the biofilm culturing period is obviously shortened, and by introducing molybdenum disulfide and a composite conductive structure thereof, a surface micro-conductive network is established, and the microbial metabolism efficiency under aerobic and facultative conditions is improved.
Owner:INNER MONGOLIA UNIV OF SCI & TECH

Strong promoter P7 suitable for streptomyces and application thereof

PendingCN121852379AEfficient gene transcriptionEfficient expressionBacteriaMicroorganism based processesMetaboliteNucleotide
The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.
Owner:SHANGHAI JIAOTONG UNIV +1

Method for treating ammonia-nitrogen wastewater with low carbon-nitrogen ratio

The invention relates to the technical field of sewage treatment, and discloses a low-carbon-nitrogen-ratio ammonia-nitrogen wastewater treatment method, which comprises the following steps: measuring the carbon source concentration, ammonia-nitrogen concentration, flow and temperature of wastewater, inputting the carbon source and ammonia-nitrogen concentration into a prediction model to obtain a microbial activity predicted value, and determining the carbon source dosage based on the difference between the predicted value and an activity threshold value; converting the carbon source feeding amount into a reference feeding rate, and performing compensation correction on the denitrification rate through a temperature correction function to obtain an initial carbon source feeding rate; taking the subsequently monitored nitrogen-containing pollutant concentration of the effluent of the denitrification area as a feedback signal, comparing the feedback signal with the target concentration, and iteratively adjusting the feeding rate. According to the method, accurate matching of the carbon source feeding amount and the microbial metabolism requirement is achieved, the problems of unstable denitrification efficiency and secondary pollution caused by inaccurate carbon source feeding in a traditional process are effectively solved, and the effluent quality guarantee capacity is remarkably improved.
Owner:SUZHOU SUWATER ENVIRONMENTAL SCI & TECH CO LTD

Application of celosia cristata in preparation of soil conditioner and soil conditioner

The invention discloses application of leguminous green manure in preparation of a multifunctional soil conditioner for improving soil enzyme activity, relieving soil microbial metabolism limitation and / or enhancing a soil ecosystem. The leguminous green manure is small crown flowers. By means of the controllable decomposition characteristic and specific secretions, the activity of soil carbon (BG and CBH), nitrogen (NAG and LAP) and phosphorus (AKP) circulation key enzymes can be remarkably enhanced, the activity of the AKP is improved by 406%, the activity of the BG is improved by 158%, the microbial carbon limitation is effectively relieved by 12%, and the resource utilization strategy is optimized. The microcelosia cristata treatment can synchronously improve the soil ecological multi-functional index (EMF) and improve the nutrient circulation synergistic efficiency. Compared with the traditional leguminous green manure, the method has the advantages that the improvement effect of the microcelosia cristata in the degraded orchard is more continuous and ecologically adaptive, and an effective and feasible new soil remediation way is provided for agricultural sustainable development.
Owner:NORTHWEST A & F UNIV

Photocatalysis-biological membrane synergistic type ecological floating bed system based on solar driving and application of photocatalysis-biological membrane synergistic type ecological floating bed system

The invention belongs to the technical field of water ecological restoration, and particularly relates to a photocatalysis-biological membrane synergistic type ecological floating bed system based on solar energy driving and application of the photocatalysis-biological membrane synergistic type ecological floating bed system. The ecological floating bed system comprises a floating bed module, a biofilm carrier module, a photocatalysis module, an aeration module and a power supply module. The carbon fiber substrate, the photocatalyst and the UV-LED lamp irradiation structure are adopted, all-day work is achieved through sunlight in the daytime and UV-LED lamps at night, the photocatalyst loaded on the carbon fiber substrate is activated, oxidation groups are generated, degradation of organic pollutants is promoted, algae are efficiently controlled, and pollutants such as algal toxin are degraded. Oxygen supply of the aeration module is enhanced, and the biological membrane nitration reaction is promoted. The composite filler in the planting basket adopts the layout of ceramsite-volcanic rock-manganese ore, so that the functions of physical adsorption, ion exchange and electron transfer are synchronously realized; after the composite filler adsorbs pollutants, in-situ regeneration can be realized through aeration disturbance and microbial metabolism; all the modules cooperate to improve the pollutant removal efficiency.
Owner:SHANGHAI ACAD OF AGRI SCI

Saline-alkali soil bio-organic fertilizer based on agricultural product processing waste and preparation method thereof

The invention belongs to the technical field of organic fertilizer preparation, and relates to a saline-alkali soil bio-organic fertilizer based on agricultural product processing waste and a preparation method thereof. 2, preparing a modified biological carrier; step 3, gradient addition of the leavening and gradient fermentation are combined; 4, mixing with humic acid, and granulating to obtain the saline-alkali soil bio-organic fertilizer. Through multi-source waste compounding, composite enzymolysis, multi-strain synergistic fermentation, modified carrier design and staged precise process control, the defects of the existing saline-alkali soil bio-organic fertilizer in the aspects of raw material utilization, decomposition efficiency, flora function, carrier performance, process control and the like are systematically overcome; the method has the core advantages that resource circulation, microbial metabolism regulation and control and nutrient requirements of the saline-alkali soil are closely combined, and efficient and stable release of nutrients is achieved.
Owner:SHANDONG ACADEMY OF AGRICULTURAL SCIENCES

Method for improving sewage treatment efficiency by using intelligent regulation and control type ecological floating bed

The invention discloses a method for improving sewage treatment efficiency by using an intelligent regulation and control type ecological floating bed, and belongs to the technical field of environmental engineering. Comprising the following steps: step 1, dividing an ecological floating bed into a plurality of functional areas, and arranging a biological signal transmission channel to enhance metabolic coupling; step 2, establishing an enzymatic reaction cascade path; step 3, monitoring and adjusting water flow and retention time in real time to realize load balancing; step 4, intelligently regulating and controlling environmental parameters and implementing a metabolic enhancement strategy to optimize the degradation efficiency; 5, the state of the ecological floating bed is evaluated regularly, performance degradation is predicted, and an automatic maintenance and optimization strategy is executed. According to the invention, the ecological floating bed is divided into a plurality of modules which are coupled with one another and have definite functional partitions, the adjustable microporous semi-permeable membrane and the differentiated carrier material are combined, and intelligent monitoring and dynamic regulation are utilized, so that collaborative optimization of microbial metabolism and environmental parameters is realized, the sewage treatment efficiency is improved, and continuous and stable operation of the system is ensured.
Owner:JIANGSU BEIFENG ECOLOGICAL AGRI TECH CO LTD

Plant extract-based superoxide dismutase bio-stress nutrient fertilizer and method of production thereof

The application discloses a superoxide dismutase biological anti-stress nutrient fertilizer based on plant extraction and a production method thereof. The fertilizer contains, in percentage by mass, 1.0-10.0% of superoxide dismutase, 1.0-5.0% of a plant anti-stress inducing factor complex, 0.1-2.0% of organic chelated trace elements, 5.0-15.0% of a phosphorus-potassium enhancing component, 0.5-5.0% of a natural protective agent, 3.0-10.0% of a penetrating agent, 0.5-15.0% of a microbial metabolic synergist, 0.1-5.0% of a pH buffer component, 0.01-0.5% of a plant-derived preservative, and the balance of water. The superoxide dismutase is preferably derived from corn germ or spinach extract. The production method comprises the steps of enzyme activity component preparation, water phase base preparation, anti-stress function phase preparation, step-by-step mixing and primary stabilization, final product integration and secondary stabilization, and the like. Through scientific compounding and synergy of various biological active components, the application significantly enhances the resistance of crops to drought, saline-alkali, high and low temperature and the like, and promotes growth and quality improvement.
Owner:HUNAN PHYTOFUNCTIONAL AGRICULTURE TECHNOLOGY CO LTD

Red soil dry land soil microbial diversity detection method based on multicolor developing test paper

The invention discloses a red soil dry land soil microbial diversity detection method based on multicolor developing test paper, and belongs to the technical field of soil environment microbial detection. According to the method, test paper loaded with different color developing agents is designed, a specific color developing reaction is triggered by combining microbial metabolic activity, and color developing characteristics are converted into parameters such as microbial diversity indexes and dominant species proportions by utilizing an image analysis model. The detection device comprises an image acquisition module and a data analysis module, and quantitative association of color development features and diversity is realized through a machine learning model. Compared with the prior art, the method has the advantages that the cost is extremely low, the detection time is shortened to be within 30 minutes, the method is suitable for field real-time monitoring of the red soil dry land, an efficient, low-cost and portable detection tool is provided for soil health management, and the method is particularly suitable for soil health assessment of resource-limited areas.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +3

Method for producing target substance

PCT designated stageWO2025254198A1BacteriaHydrolasesBiotechnologyHigh concentration
The present disclosure relates to a novel technique capable of highly efficiently producing a high-concentration preparation of a target substance in a production process for the target substance utilizing a microorganism under anaerobic conditions or microaerobic conditions, wherein a necessary substrate is efficiently supplied to a microbial metabolic pathway involved in the biosynthesis of the target substance and the dilution of the target substance in a reaction solution is suppressed. Specifically, the present disclosure relates to a method for producing a target substance, the method including (q) culturing or reacting cells of a microorganism having a urease activity or a treatment product of the cells in a culture medium or reaction medium (X) under anaerobic conditions or microaerobic conditions to produce the target substance, wherein, during the step (q), a urea-containing solution (Y) is added to the culture medium or reaction medium (X).
Owner:GREEN EARTH INST CO LTD +1

Use of undecylenic rhodin in inhibiting rhizoctonia solani

The application discloses application of undecyl-prodigiosin in inhibition of a root rot pathogen (Fusarium oxysporum) of Salvia miltiorrhiza, and belongs to the technical field of microbial pesticides. 25 H 33 The compound has a molecular formula of C 50 N3O, is derived from Streptomyces aurantiacus YS-4, has a concentration-dependent inhibitory effect on the Fusarium oxysporum, an EC of 33.1 μg / mL, and an inhibition rate of 83.3% at 400 μg / mL. The application further provides a preparation containing the compound, wherein the concentration of the compound is not less than 20 μg / mL, and the concentration of the target area after application is controlled in a range of 20 μg / mL to 400 μg / mL, so that the root rot of Salvia miltiorrhiza can be effectively prevented and treated. As a natural microbial metabolite, the compound is environment-friendly, has no residue, and is not easy to produce drug resistance, solves the pollution problem of chemical prevention and treatment, and provides a new scheme for green prevention and treatment of the root rot of Salvia miltiorrhiza.
Owner:HENAN AGRICULTURAL UNIVERSITY +2

Oligosaccharide compositions and methods of use thereof for treating viral infections

Aspects of the disclosure relate to oligosaccharide compositions and methods of making the same. Also provided are methods of using oligosaccharide compositions as microbiome metabolic therapies for treating respiratory viral illnesses, such as COVID-19.
Owner:KALEIDO BIOSCIENCES INC +1

Saccharomyces cerevisiae engineering strain with enhanced isopentenol tolerance and conversion ability and construction method and application thereof

The application belongs to the field of microbial metabolic engineering, and discloses a Saccharomyces cerevisiae engineering strain with enhanced isopentenol tolerance and conversion capacity, and a construction method and application thereof. The Saccharomyces cerevisiae engineering strain is obtained by modifying an isopentenol utilization pathway-dependent Saccharomyces cerevisiae strain, and the energy metabolism of the Saccharomyces cerevisiae strain is enhanced to synthesize a large amount of ATP for the isopentenol utilization pathway; and the antioxidant stress resistance of the Saccharomyces cerevisiae strain is enhanced to relieve the oxidative damage caused by the isopentenol substrate. The Saccharomyces cerevisiae engineering strain is the strain with the highest efficiency in converting isopentenol into terpenes.
Owner:SOUTH CHINA UNIV OF TECH

Application of an acid control agent in fermented pancakes

This invention relates to the field of food processing technology and provides an application of an acid-controlling agent in fermented pancakes. The acid-controlling agent is nisin and / or ε-polylysine. It is used to control the acidity of the fermented pancakes. Specifically, the acid-controlling method for fermented pancakes involves adding the acid-controlling agent after fermentation is complete and the pH value has been neutralized with alkali to obtain the fermented pancake batter. This method can precisely inhibit the continued fermentation of the pancake batter during the spreading stage, achieving precise intervention in the fermentation process and microbial metabolism. The acid-controlling effect is significant, the sensory quality is noticeably improved, and the acidity of the pancake batter remains stable, ensuring consistency with the flavor of the final product. This method is suitable for the large-scale production of fermented pancakes.
Owner:JILIN UNIVERSITY

AOA sewage treatment intelligent control system based on digital twinning

The present application relates to the field of sewage treatment, and more particularly to an AOA sewage treatment intelligent control system based on digital twinning. It comprises a physical perception layer, a data transmission layer, a digital twinning layer, an intelligent control layer and an execution layer. The physical perception layer collects AOA process physical data throughout the whole process and preprocesses them into standardized data; the data transmission layer realizes the bidirectional transmission of data and control instructions between each level; the digital twinning layer, based on the denitrification and phosphorus removal microbial metabolism mechanism of the AOA process, constructs a whole-process twinning model synchronized with the physical system in real time and generates simulation prediction data; the intelligent control layer generates control instructions according to the data; the execution layer executes the instructions and collects feedback data, which is used to update the twinning model and correct the control instructions. Through the five-layer series closed-loop structure, the present application realizes the intercommunication of data, the interaction of instructions and the closed-loop feedback of each level, and constructs a whole-process closed-loop intelligent control system of "perception-transmission-twinning-decision-execution".
Owner:ZHONGJIAO ROAD & BRIDGE (HEBEI) CO LTD

Saline-alkali land original soil raw water double-micro-flooding root-promoting technology

The invention discloses a saline-alkali soil raw water double-micro-flooding root-promoting technology. According to the method, 150-1500 kg of a soil conditioner, 30-150 kg of a soil remediation agent and 450-1500 kg of a soil biological conditioning fertilizer are added into per hectare of original soil according to the actual salinity condition of farmland soil. According to the actual condition of crop cultivation, ditching, ridging or bedding, drip irrigation tape laying, irrigation and seeding are carried out; and the halophilic bacteria agent is conveyed and applied through a drip irrigation belt after being uniformly stirred. By synergistically promoting metabolism of halophilic microorganisms to remove salt and improve rhizosphere microenvironment of crops, root growth is facilitated, saline-alkaline resistance of the crops is improved, and high yield and high quality of the crops are achieved. The technology does not need soil dressing, needs to consume a large amount of fresh water resources, does not have complex treatment engineering, and saves cost investment. The saline-alkali soil is improved gradually and effectively while the yield and economic benefits are obtained freely through saline-alkali soil planting, and a breakthrough positive contribution is made to planting crops on the saline-alkali soil.
Owner:GUANGDONG OCEAN UNIVERSITY +2

Method and device for producing biogas by anaerobic fermentation of sludge using microwave heating under the action of a magnetic field

The present disclosure provides a method and device for heating sludge anaerobic fermentation to produce biogas by microwaves under the action of a magnetic field, and relates to the technical field of green energy. The method comprises: drying and crushing sludge raw materials and inoculating anaerobic bacteria to form a fermentation mixture; placing the fermentation mixture in a closed reactor equipped with a microwave heating system and an electromagnetic field generating system, forming a uniform temperature field by microwaves and applying a controllable magnetic field to stimulate microbial activity; periodically switching or cooperatively controlling the two systems to maintain the target temperature zone and realize dynamic metabolic reinforcement; detecting the biogas yield and components, and adjusting the energy field parameters to form a closed loop control accordingly. The present scheme can establish a dynamic coupling between energy input and microbial metabolism, make the internal temperature distribution of the system uniform, the reaction rate stable, and the energy utilization rate high, and further make the organic matter degradation more sufficient and the gas production process more continuous, so that the energy efficiency and controllability of the overall anaerobic fermentation can be significantly improved.
Owner:NORTHWEST ENGINEERING CORPORATION LIMITED