275 results about "Microbial metabolism" patented technology

The invention belongs to the technical field of water treatment, and particularly provides a nitrogen-oxygen co-doped hypha / spruce composite adsorption material and a copper-containing sewage treatment method. According to the invention, the copper-containing sewage is pretreated by using the solid fermentation product, and then the copper-containing sewage is post-treated by using the nitrogen-oxygen co-doped hypha / spruce composite adsorption material prepared under specific conditions; wherein in the pretreatment stage, microbial metabolites in a solid fermentation product preliminarily capture free copper ions (Cu < 2 + >) through complexation, the toxicity of the sewage is reduced, and meanwhile, the copper ions are partially converted into a form which is easier to adsorb. In the post-treatment stage, the nitrogen-oxygen co-doped hypha / spruce composite adsorption material plays a key role, and efficient interception and adsorption of copper ions are achieved. In conclusion, the treatment method for the copper-containing sewage comprises a pretreatment stage and a post-treatment stage, the two stages are in synergistic interaction, and finally the removal rate of the heavy metal copper can be effectively increased.

The invention discloses a preparation method of a composite slow-release carbon source, which comprises the following steps: preparing an inner core: uniformly dry-mixing lignin powder and a quick-acting carbon source, and adding a first binder to prepare inner core particles; middle layer wrapping: alternately spraying a second binder and middle layer coating powder until the middle layer is completely wrapped to obtain double-layer particles; outer layer wrapping: alternately spraying a third binder and outer layer coating powder until the outer layer is completely wrapped to obtain three layers of particles; and spraying a biodegradable polymer solution on the surfaces of the three layers of particles to form a coating film. Through the three-layer structural design of the quick start layer, the stable maintenance layer and the long-acting guarantee layer, accurate matching of carbon source release and microbial metabolism requirements is realized. The outer-layer quick-acting carbon source quickly starts microbial activity, the middle layer provides stable and continuous carbon source supply, and the inner core ensures the tail-section treatment effect. The sequential release mechanism effectively avoids burst release waste of the carbon source, so that the utilization rate of the carbon source is greatly improved.

The invention discloses a central air conditioner pollution degree autonomous evaluation robot based on multi-modal perception, and relates to the technical field of intelligent detection, the robot comprises a voxel space division module, a data acquisition module, a pollution characteristic mapping modeling module, a physical pollution detection module, a chemical pollution detection module and a biological pollution detection module; through three-dimensional point cloud data scanning and real-time data acquisition, the air pipe wall surface roughness, the temperature delay time, the gas fluctuation, the heat retention degree and the microbial metabolism gas concentration are monitored in real time, and a three-dimensional pollution characteristic mapping model is generated according to the pollution characteristic data set; by calculating a physical pollution index PPI, a chemical pollution index CPI and a biological pollution index BPI and comparing and analyzing with corresponding threshold values, whether dust retention, gas concentration and microorganism quantity in an air pipe exceed standards or not is judged in real time, cleaning, filtering and sterilizing strategies are automatically triggered, and the air quality and operation safety of a central air conditioning system are effectively guaranteed.

The invention relates to the field of microbial metabolism engineering, and discloses a method for increasing the yield of 2 '-fucosyllactose (2'-FL) through the synergistic effect of various alpha-1, 2-fucosyltransferases. According to the method, two or more alpha-1, 2-fucosyltransferases with different sources are co-expressed in microbial cells, and the complementary enzymatic characteristics of the alpha-1, 2-fucosyltransferases are utilized, so that the synthesis efficiency of the 2 '-FL is remarkably improved, and the yield of the 2'-FL is effectively improved. The microbial cell is escherichia coli C43 (DE3) (E. coli C43 lacZ waaF with lacZ and waaF genes knocked out, the serial number is SL), a pETDuet-1 plasmid is constructed, and genes manB, manC, gmd, wcaG and zwf are overexpressed on a pETDuet-1 vector, so that the microbial cell can be used for preparing the microbial cell. In a 5L fermentation tank, after the final strain SL07 is used for final fermentation for 68 hours, the yield of 2 '-FL reaches 148g / L, and the production intensity reaches 2.18 g / L / h. The embodiment shows that the engineering strain for co-expressing the alpha-1, 2-fucosyltransferase from different sources can obviously improve the yield of the 2 '-FL.

The invention discloses a method and system for analyzing a bacterium-drug interaction panoramic dynamic mechanism by using a causal enlightenment hypergraph neural network, and electronic equipment, and belongs to the technical field of artificial intelligence and biomedical informatics. The method comprises the following steps: constructing a disease / syndrome / disease-bacterium / microorganism / metabolite-prescription / drug / component interlocking hypergraph; analyzing a bacteria-drug interaction dynamic mechanism based on a dynamic hypergraph network algorithm; optimizing the hypergraph neural network based on the prescription compatibility rule and the traditional Chinese medicine dosage; and predicting the microbial marker based on the causal enlightenment hypergraph neural network. According to the method, a multi-entity high-order relation is captured through an interlocking hypergraph structure, a time sliding window and an attenuation mechanism are introduced to analyze dynamic evolution, the traditional Chinese medicine dosage is used as an attention priori optimization network, and causal reasoning is achieved through alternate optimization of an intervention discovery and response prediction module. In a public data set test, the performance evaluation index is obviously superior to that in the prior art, and the problem of analyzing the panoramic dynamic mechanism of the bacterium-drug interaction is effectively solved.

The invention provides a functional composite suspended biological carrier and a preparation and application method thereof. The functional composite suspended biological carrier comprises a main base material and functional components, the main body base material is polyethylene or polypropylene, and the functional component comprises the following substances in parts by mass: 3.0 parts of polyquaternium-10 or cationic polyacrylamide, 1.5 parts of molybdenum disulfide, 2.5 parts of vinyl acetate or acrylate, 2.0 parts of nano silicon dioxide, 2-3 parts of talcum powder, 0.5 part of a silane coupling agent and 0.5 part of polyethylene wax; the total proportion of inorganic components in the carrier is less than or equal to 12%; the cross section of the carrier is a 19-hole honeycomb hollow cylinder, the diameter of the formed cylinder is 25 mm, the height of the formed cylinder is 10 mm, and the density of the carrier is 0.95-1.00 g / cm; according to the invention, the state of charge on the surface of the carrier is adjusted through the positive electricity polymer PQAS-10, the affinity with negatively charged microorganisms is enhanced, the biofilm culturing period is obviously shortened, and by introducing molybdenum disulfide and a composite conductive structure thereof, a surface micro-conductive network is established, and the microbial metabolism efficiency under aerobic and facultative conditions is improved.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention relates to the technical field of sewage treatment, and discloses a low-carbon-nitrogen-ratio ammonia-nitrogen wastewater treatment method, which comprises the following steps: measuring the carbon source concentration, ammonia-nitrogen concentration, flow and temperature of wastewater, inputting the carbon source and ammonia-nitrogen concentration into a prediction model to obtain a microbial activity predicted value, and determining the carbon source dosage based on the difference between the predicted value and an activity threshold value; converting the carbon source feeding amount into a reference feeding rate, and performing compensation correction on the denitrification rate through a temperature correction function to obtain an initial carbon source feeding rate; taking the subsequently monitored nitrogen-containing pollutant concentration of the effluent of the denitrification area as a feedback signal, comparing the feedback signal with the target concentration, and iteratively adjusting the feeding rate. According to the method, accurate matching of the carbon source feeding amount and the microbial metabolism requirement is achieved, the problems of unstable denitrification efficiency and secondary pollution caused by inaccurate carbon source feeding in a traditional process are effectively solved, and the effluent quality guarantee capacity is remarkably improved.

The application discloses a superoxide dismutase biological anti-stress nutrient fertilizer based on plant extraction and a production method thereof. The fertilizer contains, in percentage by mass, 1.0-10.0% of superoxide dismutase, 1.0-5.0% of a plant anti-stress inducing factor complex, 0.1-2.0% of organic chelated trace elements, 5.0-15.0% of a phosphorus-potassium enhancing component, 0.5-5.0% of a natural protective agent, 3.0-10.0% of a penetrating agent, 0.5-15.0% of a microbial metabolic synergist, 0.1-5.0% of a pH buffer component, 0.01-0.5% of a plant-derived preservative, and the balance of water. The superoxide dismutase is preferably derived from corn germ or spinach extract. The production method comprises the steps of enzyme activity component preparation, water phase base preparation, anti-stress function phase preparation, step-by-step mixing and primary stabilization, final product integration and secondary stabilization, and the like. Through scientific compounding and synergy of various biological active components, the application significantly enhances the resistance of crops to drought, saline-alkali, high and low temperature and the like, and promotes growth and quality improvement.

The invention discloses a red soil dry land soil microbial diversity detection method based on multicolor developing test paper, and belongs to the technical field of soil environment microbial detection. According to the method, test paper loaded with different color developing agents is designed, a specific color developing reaction is triggered by combining microbial metabolic activity, and color developing characteristics are converted into parameters such as microbial diversity indexes and dominant species proportions by utilizing an image analysis model. The detection device comprises an image acquisition module and a data analysis module, and quantitative association of color development features and diversity is realized through a machine learning model. Compared with the prior art, the method has the advantages that the cost is extremely low, the detection time is shortened to be within 30 minutes, the method is suitable for field real-time monitoring of the red soil dry land, an efficient, low-cost and portable detection tool is provided for soil health management, and the method is particularly suitable for soil health assessment of resource-limited areas.

The application discloses application of undecyl-prodigiosin in inhibition of a root rot pathogen (Fusarium oxysporum) of Salvia miltiorrhiza, and belongs to the technical field of microbial pesticides. 25 H 33 The compound has a molecular formula of C 50 N3O, is derived from Streptomyces aurantiacus YS-4, has a concentration-dependent inhibitory effect on the Fusarium oxysporum, an EC of 33.1 μg / mL, and an inhibition rate of 83.3% at 400 μg / mL. The application further provides a preparation containing the compound, wherein the concentration of the compound is not less than 20 μg / mL, and the concentration of the target area after application is controlled in a range of 20 μg / mL to 400 μg / mL, so that the root rot of Salvia miltiorrhiza can be effectively prevented and treated. As a natural microbial metabolite, the compound is environment-friendly, has no residue, and is not easy to produce drug resistance, solves the pollution problem of chemical prevention and treatment, and provides a new scheme for green prevention and treatment of the root rot of Salvia miltiorrhiza.

Aspects of the disclosure relate to oligosaccharide compositions and methods of making the same. Also provided are methods of using oligosaccharide compositions as microbiome metabolic therapies for treating respiratory viral illnesses, such as COVID-19.

This invention relates to the field of food processing technology and provides an application of an acid-controlling agent in fermented pancakes. The acid-controlling agent is nisin and / or ε-polylysine. It is used to control the acidity of the fermented pancakes. Specifically, the acid-controlling method for fermented pancakes involves adding the acid-controlling agent after fermentation is complete and the pH value has been neutralized with alkali to obtain the fermented pancake batter. This method can precisely inhibit the continued fermentation of the pancake batter during the spreading stage, achieving precise intervention in the fermentation process and microbial metabolism. The acid-controlling effect is significant, the sensory quality is noticeably improved, and the acidity of the pancake batter remains stable, ensuring consistency with the flavor of the final product. This method is suitable for the large-scale production of fermented pancakes.

The present application relates to the field of sewage treatment, and more particularly to an AOA sewage treatment intelligent control system based on digital twinning. It comprises a physical perception layer, a data transmission layer, a digital twinning layer, an intelligent control layer and an execution layer. The physical perception layer collects AOA process physical data throughout the whole process and preprocesses them into standardized data; the data transmission layer realizes the bidirectional transmission of data and control instructions between each level; the digital twinning layer, based on the denitrification and phosphorus removal microbial metabolism mechanism of the AOA process, constructs a whole-process twinning model synchronized with the physical system in real time and generates simulation prediction data; the intelligent control layer generates control instructions according to the data; the execution layer executes the instructions and collects feedback data, which is used to update the twinning model and correct the control instructions. Through the five-layer series closed-loop structure, the present application realizes the intercommunication of data, the interaction of instructions and the closed-loop feedback of each level, and constructs a whole-process closed-loop intelligent control system of "perception-transmission-twinning-decision-execution".

The present disclosure provides a method and device for heating sludge anaerobic fermentation to produce biogas by microwaves under the action of a magnetic field, and relates to the technical field of green energy. The method comprises: drying and crushing sludge raw materials and inoculating anaerobic bacteria to form a fermentation mixture; placing the fermentation mixture in a closed reactor equipped with a microwave heating system and an electromagnetic field generating system, forming a uniform temperature field by microwaves and applying a controllable magnetic field to stimulate microbial activity; periodically switching or cooperatively controlling the two systems to maintain the target temperature zone and realize dynamic metabolic reinforcement; detecting the biogas yield and components, and adjusting the energy field parameters to form a closed loop control accordingly. The present scheme can establish a dynamic coupling between energy input and microbial metabolism, make the internal temperature distribution of the system uniform, the reaction rate stable, and the energy utilization rate high, and further make the organic matter degradation more sufficient and the gas production process more continuous, so that the energy efficiency and controllability of the overall anaerobic fermentation can be significantly improved.

The application discloses a kind of based on carbon-based modified electrode microbial quantity dynamic evaluation method.The method comprises the following steps: preparing carbon-based modified electrode doped with target microorganism;Carbon-based modified electrode is used as working electrode, and reference electrode and counter electrode constitute three-electrode system, and is placed in the medium containing target microorganism to be measured in the electrochemical scanning of target microorganism, and the electrochemical response signal is collected;The electrochemical response signal is processed, and the characteristic electrochemical parameter of target microorganism metabolite is obtained;According to the preset detection time threshold selection model: when detection time is less than or equal to preset duration, linear model is used;When detection time is greater than preset duration, dynamic metabolism model is used;Characteristic electrochemical parameter is substituted into the selected model, and the number of microorganisms in the medium to be measured is calculated.The application can realize accurate quantification, anti-interference detection and rapid dynamic evaluation of the number of microorganisms, and significantly improve detection efficiency and accuracy.

This invention belongs to the field of microbial metabolic engineering and synthetic biology, specifically relating to a strain for high-yield... R,R -2,3-Butanediol ( R,R Salt-tolerant Jeju Bacillus DT01 IM3 DLB6 (-2,3-BDO), production strain constructed from this strain and its application in fermentation production. R,R Applications of -2,3-BDO. This invention screens a high-yielding strain through heavy ion beam irradiation mutagenesis. R,R Jeju Bacillus DT01 IM3 DL B6 with -2,3-BDO, preservation number CGMCC No. 37311. This strain exhibits excellent salt tolerance and low endotoxin properties. The production strain constructed from it has extremely high product synthesis capabilities, enabling high-concentration, high-intensity industrial fermentation production.

This invention discloses a fermentation monitoring and early warning system and method, relating to the field of monitoring and early warning technology. The fermentation monitoring and early warning system includes a data acquisition module, a data analysis module, an early warning monitoring module, and an alarm module. The data acquisition module acquires monitoring data in real time. The data analysis module calculates the dissolved carbon dioxide concentration in the liquid, corrects the dissolved carbon dioxide concentration to obtain an effective dissolved carbon dioxide concentration, and calculates an early warning index based on temperature, pH value, dissolved oxygen concentration, and effective dissolved carbon dioxide concentration. The early warning monitoring module acquires the early warning index, compares the early warning index value with a preset threshold range, and triggers a corresponding early warning level to generate a corresponding early warning signal based on the comparison result. The alarm module receives the early warning signal and outputs an alarm prompt corresponding to the early warning signal. This invention can reduce the risk of false alarms or missed alarms, enabling operators to intervene in a timely manner before the microbial metabolic balance is disrupted.

The present application belongs to the field of microbial metabolic engineering and synthetic biology technology application, in particular to a high methanol tolerance yeast chassis strain, a high 3-hydroxypropionic acid yield engineering bacterium and application. The high methanol tolerance yeast chassis strain takes wild type Pichia pastoris or fatty acid producing Pichia pastoris as a starting strain, is modified according to at least one of the following conditions (a)-(h), and a high methanol tolerance strain is obtained, wherein (a) MIOX gene knockout; (b) DMA2 gene knockout; (c) MET17 gene knockout; (d) BUB2 gene knockout; (e) CLB4 gene knockout; (f) UBP12 gene knockout; (g) ZFP gene knockout; (h) AOX1 gene mutation. The high methanol tolerance yeast chassis strain is further modified to obtain a high 3-hydroxypropionic acid yield engineering bacterium. The present application combines rational and non-rational metabolic engineering strategies, realizes efficient biosynthesis of fatty acids and 3-hydroxypropionic acid with methanol as the only carbon source, and lays an important foundation for low-carbon green biological manufacturing of chemicals.

The invention discloses a black pepper extract-containing composition capable of promoting intestinal digestion and absorption and a cat food, and relates to the technical field of animal foods.The composition is prepared by compounding fish oil, krill oil, sunflower seed oil and linseed oil according to a specific proportion based on compound functional grease and natural plant extracts in a scientific proportion; according to the invention, the ratio of omega6 fatty acid to omega3 fatty acid is optimized to an ideal range of (2.5-3.2): 1, black pepper extract with functions of promoting digestion and regulating flora is synergistically added, and through combination of scientifically proportioned functional components and an advanced process, an'intestinal tract-microorganism-metabolism 'experiment verifies that digestion and absorption of cat nutrition are realized, and the effect of promoting digestion and regulating flora is achieved. Meanwhile, the multi-dimensional and systematic improvement of oxidation resistance, inflammation resistance, immunity improvement and metabolism functions is achieved.

The present application relates to the field of groundwater environment numerical simulation, and more particularly to a method for mixed dynamic simulation and prediction of water chemistry and microbial field. It comprises: obtaining geological and hydrodynamic data and discrete biochemical parameters to construct an initial multi-modal data set, establishing a multi-field coupled mathematical model containing seepage migration and microbial metabolism based on the data set, constructing a physical information neural network, embedding the model as a constraint into a loss function to reconstruct the initial flow field and biomass field, then starting a time step iteration to receive dynamic boundary conditions to update the current flow field and medium permeability and feed back to the next step calculation, and finally constructing a Bayesian neural network to predict the three-dimensional state distribution and cut off the evolution process when the cognitive uncertainty index exceeds the preset threshold. The present application improves the numerical stability of complex underground multi-field dynamic deduction and effectively improves the long-period cumulative error divergence.

The invention discloses an automatic mixed fermentation system and process for preparing a traditional Chinese medicine multi-strain fermented beverage, and relates to the technical field of beverage production and fermentation. According to the automatic mixed fermentation system and process for preparing the traditional Chinese medicine multi-strain fermented beverage, through cooperation of the mixing frame mechanism, the driving mechanism, the cam frame mechanism, the gas pumping and discharging mechanism and the solution pumping and discharging mechanism, the driving mechanism can drive the mixing frame mechanism to rotate, liquid traditional Chinese medicine and various probiotics in the fermentation barrel are stirred and mixed, and the liquid traditional Chinese medicine and the various probiotics are mixed; the gas pumping and discharging mechanism and the solution pumping and discharging mechanism can also be driven to work, so that the gas pumping and discharging mechanism pumps external gas into the fermentation barrel, the dissolved oxygen amount in materials in the fermentation barrel is controlled, the dissolved oxygen requirement in the liquid state fermentation process is met, the solution pumping and discharging mechanism pumps external acid-base adjusting liquid into the fermentation barrel, and the fermentation efficiency is improved. The pH value of the material is maintained in a required range, the large fluctuation of the dissolved oxygen amount and the pH value caused by microbial metabolism in the fermentation process is prevented, and stable liquid fermentation is guaranteed.

The invention discloses a method for rapidly predicting microbial contamination of meat based on multi-modal fusion. The method comprises the following steps: acquiring spectrum and smell data on the surface of meat, extracting characteristics related to microbial metabolism, and establishing a quantitative relationship between the characteristics and the content of microorganisms (such as the total number of bacterial colonies) by utilizing a machine learning model, thereby realizing rapid and lossless prediction on microbial pollution. According to the method, the defect of long period of a traditional microorganism detection method is overcome, and an effective and rapid monitoring means is provided for microorganism safety risks in the meat production and circulation process.

The application discloses a method for improving hydrogen production performance of Clostridium butyricum by using hematite nanoparticles, and belongs to the technical field of biological hydrogen production and microbial metabolism regulation. The method improves the hydrogen production performance by adding hematite nanoparticles into an anaerobic fermentation hydrogen production system of Clostridium butyricum for anaerobic fermentation. The application first discloses a specific double regulation mechanism of hematite nanoparticles on hydrogen production of Clostridium butyricum. By using hematite nanoparticles as a core regulation material, and through precise concentration gradient addition and stage temperature synergistic regulation, the hydrogen production lag period can be greatly shortened, the initial hydrogen production rate can be improved, byproduct synthesis of butyric acid can be inhibited, and the efficient hydrogen production path of acetic acid can be strengthened. The core technical problems that existing iron-based additives cannot shorten the hydrogen production lag period, the initial hydrogen production efficiency is low, and product feedback inhibition is easily caused are solved. The method is simple to operate, has strong industrialization adaptability, and can be widely applied to industrialization batch and continuous production of dark fermentation biological hydrogen production.

The invention relates to a preparation method and application of an iron ore-carrier water treatment material, and belongs to the technical field of water treatment and water environment remediation, the preparation method comprises the following steps: grinding an iron ore material with a grinding medium to obtain iron ore powder, and storing the iron ore powder in a nitrogen environment for later use; the method comprises the following steps: mixing iron ore powder with a carrier supporting material, adding water, stirring and filtering to obtain a product A; the product A is subjected to heating, heat preservation, cooling, crushing and screening in the nitrogen atmosphere, and then the iron ore-carrier water treatment material is obtained. The water treatment material prepared by using reduction components such as iron, aluminum and sulfur in an iron ore material and combining with a carrier supporting material through a specific heat treatment process has better physicochemical characteristics, provides a good habitat for microorganisms, and not only promotes microbial metabolism to degrade organic pollutants, but also improves the water treatment efficiency by combining with a biofilm formation technology. And pollutants are purified through a chemical reduction effect, so that the removal efficiency of various pollutants is improved.

This invention discloses a method for promoting the biodegradation of N,N-dimethylformamide and simultaneous denitrification. The method constructs a zero-valent iron (ZFE)-coupled biological treatment system, which not only accelerates electron transfer efficiency but also significantly increases the activity of DMF-degrading enzymes and nitrate reductases, synergistically promoting electron transfer and microbial metabolic activity, thereby enhancing the removal of DMF and nitrate nitrogen. Compared to biological treatment systems that only add activated sludge without adding ZFE, adding ZFE significantly improves the removal efficiency of DMF and nitrate nitrogen, making it suitable for the treatment of large-scale polluted water bodies or industrial wastewater.

The invention discloses temperature-sensitive-oxygen-release dual-mode hydrogel cooperatively regulated by oxygen and microorganisms and application of the temperature-sensitive-oxygen-release dual-mode hydrogel in aerobic composting, and belongs to the technical field of organic solid waste treatment and recycling. The technical problem that in the aerobic composting process, released oxygen is not matched with microbial metabolism requirements is solved. The temperature-sensitive-oxygen-release dual-mode hydrogel prepared by the invention is of a spherical structure and sequentially comprises a CaO2 core, an EC film for wrapping the CaO2 core, a temperature-sensitive hydrogel shell for wrapping the EC film and a compound microbial agent positioned on the temperature-sensitive hydrogel shell from inside to outside. The temperature-sensitive material responds to the temperature change of the pile body, when the pile temperature reaches 45 DEG C, the shell is triggered to shrink, the isolating layer is destroyed, oxygen is released and thermophilic bacteria are activated, on-demand oxygen supply and microbial agent controlled release are achieved, the problem of local oxygen deficit of the pile body can be effectively solved, the composting efficiency and quality are improved, and the method is suitable for treatment of organic solid waste such as agricultural waste and municipal sludge.

The application discloses Lactobacillus delbrueckii SWU-DLB2 and SWU-DLB3 and development and application thereof in functional fermented food, and belongs to the technical field of microbial fermentation. The Lactobacillus delbrueckii SWU-DLB2 and SWU-DLB3 have been preserved in the Guangdong Microbial Culture Collection Center on October 28, 2025, and the preservation numbers are GDMCC No: 67173 and GDMCC No: 67174 respectively. It is found through experiments that the fermentation of the two strains mentioned above in dairy products containing Zizyphus jujuba Mill. powder can significantly improve the content of bioactive substances, and the total phenol content, flavonoid content and antioxidant activity are all significantly improved. The Lactobacillus delbrueckii strain provided by the application is combined with a plant-based supplement and applied to a food model, a functional food with enhanced neuroprotective potential is developed, and the important role of microbial metabolism in the functionalization of food is highlighted.

The utility model discloses a chemical fiber cloth winding device capable of preventing folding and wrinkling, and particularly relates to the technical field of chemical fiber cloth production equipment, a supporting plate is arranged on the right side of the upper end of a base, a disinfection treatment box is arranged on the left side of a guide roller, and heating guide rollers are arranged on the opposite sides of a discharging port and a feeding port. Ultraviolet lamps are mounted on two sides of an inner cavity of the disinfection treatment box; an alcohol barrel is arranged on the left side of an alcohol pump; winding rollers are mounted at the upper ends of opposite surfaces of the two groups of bearing plates; the cloth is disinfected through the disinfection treatment box before being wound, so that the number of microorganisms such as bacteria and molds on the cloth is greatly reduced, the breeding speed of the microorganisms can be inhibited, and therefore the risk that the cloth is eroded by acidic substances, pigments and the like generated by microbial metabolism is reduced, and the service life of the cloth is prolonged. The problems of discoloration, deterioration, mildewing and the like of the cloth are prevented, the storage time of the cloth is prolonged, the chemical fiber cloth can be quickly volatilized after being sprayed and disinfected, and the quality of the cloth is kept.

This invention relates to the technologies of genetic engineering and fermentation engineering, and more specifically, to a method for improving the production of metabolites by heterotrophic microorganisms through the reconstruction of the Calvin cycle. In this invention, by adjusting the expression of Calvin cycle-related genes and reconstructing the Calvin cycle in heterotrophic microorganisms, the metabolism of heterotrophic microorganisms to lipid and carbon sources is efficiently promoted, the operation of the β-oxidation cycle is accelerated, and the ability of heterotrophic microorganisms to synthesize target metabolites from lipid and carbon sources is significantly improved. Through the strategy of adjusting Calvin cycle-related genes in this invention, the ability of microorganisms to synthesize metabolites such as PHA is significantly improved, the content and production amount of metabolites such as PHA produced by microorganisms through fermentation are improved, and a new modification target and method can be provided for the construction of genetically engineered microorganisms for target metabolites.