4 results about "Fed-batch culture" patented technology

The application discloses a kind of high-yield O-succinyl-L-homoserine recombinant escherichia coli and its construction method and application, belong to genetic engineering and fermentation engineering technical field.The recombinant escherichia coli is expressed by expressing anti-feedback inhibition homoserine O-succinyltransferase metA fbr , aspartate ammonia-lyase aspA, aspartate transaminase aspC, 2 ketoglutarate decarboxylase sucA, dihydrothioctic acid succinyltransferase sucB and efflux protein yjeH, knock out metJ, improve intracellular NADPH availability, to enhance OSH synthesis pathway metabolic flow, precursor succinyl coenzyme A supply and product efflux.Using 5 L bioreactor for fed-batch fermentation, after fermentation 60 h, OSH production is as high as 137.4 g / L, and sugar acid conversion rate reaches 50.5%.The application provides engineering strain and technical realization path for the efficient fermentation of OSH.

This invention discloses a high-yielding erythritol-producing *Yarrowia lipophila* strain (… Yarrowia lipolytica This invention relates to the field of biotechnology, specifically to *Yarrowia lipophila* CA20 as the starting strain, and its applications. A000039 A gene knockout method was used to construct a high-yield erythritol-producing *Yarrowia lipolytica* strain. The construction method provided by this invention is simple and of significant value in simplifying the construction process of high-yield strains. Furthermore, under the fermentation medium and conditions provided by this invention, the *Yarrowia lipolytica* strain producing high-yield erythritol was fed-batch fermented with glucose as a substrate, achieving a yield of 280.50 g / L and a yield of 0.71 g / g, demonstrating good economic benefits.

Disclosed herein are methods, cells, and compositions for the production of bacterial cell extracts. The bacterial cell extracts are used in cell-free protein synthesis of recombinant proteins and large quantities of bacterial cell extracts are typically needed. The present disclosure relates to the production of high cell density fermentations that provide large quantities of bacteria for the manufacturing of bacterial cell extracts. The present disclosure relates to high density fed batch fermentation for the production of bacterial cell extract. Also provided herein are cell-free protein synthesis systems and methods that use the bacterial cell extracts.

The application discloses a mutagenic high-yield acid sophorolipid Torulopsis globosa and a fermentation culture method. Starmerella bombicola The mutagenic high-yield acid sophorolipid Torulopsis globosa (Torulopsis globosa) strain G72 is obtained by using Torulopsis globosa ATCC 22214 as a starting strain, compound mutagenesis of UV and EMS and grading screening under high-sugar conditions, and is preserved in the Guangdong Microbial Digital Museum Center with a preservation number of GDMCC 67888. The application further provides a segmented fed-batch fermentation process for synergistically controlling high-sugar and low-oil of the mutant strain G72, through segmentally regulating a sugar-oil ratio, pH and dissolved oxygen, the end-point concentration of sophorolipid can be stabilized at about 347.94 g / L, the acid sophorolipid concentration is 267.25 g / L, the proportion is more than 80%, the fermentation period is shortened, and the high selectivity, high space-time yield and low-cost production of the acid sophorolipid are realized.