94 results about "Functional peptide" patented technology

The invention discloses keratinase Phyto-Keratinase and application of the keratinase Phyto-Keratinase. The keratinase Phyto-Keratinase comprises the following components: (1) protease which is coded by a nucleotide sequence as shown in SEQ ID NO.1; and / or (2) protease which is derived from bacillus subtilis, is coded by a nucleic acid sequence with more than 98% of identity with the SEQ ID NO.1 nucleotide sequence and has the characteristic of hydrolyzing keratin. According to the plant keratinase Phyto-Keratinase disclosed by the invention, the Phyto-Keratinase (plant keratinase) produced by utilizing microbial fermentation is a serine proteolytic enzyme, is also a biocompatible cutinase, and is obtained by fermenting saccharomyces cerevisiae. Phyto-Keratinase provides a nitrogen source for the growth of plants (beans), and contains various amino acids (functional peptides) and special proteolytic enzymes derived from the plants at the same time. The Phyto-keratinase can provide the effects of resisting inflammation, removing cutin and providing nutrition for skin regeneration at the same time, can relieve various inflammations by inhibiting PAR-2 receptors, and has potential value in the fields of cosmetics, beauty medicine and environment.

The invention belongs to the technical field of walnut protein preparation, and particularly relates to walnut protein powder with high digestibility, low sensitization and low bitterness as well as a preparation method and application of the walnut protein powder. According to the preparation method of the walnut protein powder with high digestibility, low sensitization and low bitterness provided by the invention, proline specific endo protease, recombinant trypsin and carboxypeptidase B are cooperatively used, so that accurate damage to an anti-digestion structure and a sensitization epitope of walnut protein can be realized; according to the method, ultrahigh digestibility (greater than or equal to 92%), thorough desensitization (greater than 99.8%), bitterness inhibition and functional peptide enrichment are synchronously realized, so that the defects of low digestibility, sensitization residue, obvious bitterness, low yield of functional peptide, complex process and the like caused by uncontrollable cutting in the existing random enzymolysis technology are overcome, and meanwhile, the core technical bottleneck of high-value utilization of walnut protein is overcome.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention provides a hair care composition with oil control, hair loss prevention and repair effects and a preparation method of the hair care composition, and belongs to the technical field of hair care. The hair care composition comprises functional peptides such as myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8, palmitoyl tetrapeptide-7 and the like which are synergistically combined according to a specific proportion, and glycerin, water and a penetration enhancer are supplemented to form a basic system. By optimizing peptide structure modification, adding proportion and the steps of pH control, ultrasonic treatment and collaborative assembly in the preparation process, multi-path collaborative regulation and control of hair follicle activation, scalp inflammation inhibition and keratin synthesis enhancement are realized.

A cage-like nanocarrier for targeted delivery of siRNA, and a preparation method therefor and the use thereof. The preparation method comprises: (A) mutating a negatively charged or uncharged amino acid on the inner surface of a ferritin into a positively charged amino acid; and any one or more of the following steps: (B) coupling the N-terminus of the ferritin to a functional peptide having nucleic acid affinity; (C) coupling the N-terminus of the ferritin to a functional peptide promoting lysosomal escape; (D) truncating the E-helix at the C-terminus of the ferritin; (E) coupling the C-terminus of the ferritin to a functional peptide having nucleic acid affinity; and (F) coupling the C-terminus of the ferritin to a functional peptide promoting lysosomal escape. A new nucleic-acid-loaded protein nanocage carrier is constructed by means of modifying a negatively charged inner cavity of ferritin to make same positively charged. By means of electrostatic adsorption, a negatively charged siRNA can be efficiently loaded into a ferritin nanocage, thereby significantly improving the in-vivo and in-vitro delivery stability of siRNA, lysosomal escape functions, and efficacy of targeted therapy.

The invention discloses a ganoderma lucidum peptide for enhancing immunity as well as a preparation method and application thereof, and belongs to the technical field of functional peptide preparation. The ganoderma lucidum peptide provided by the invention contains various active ingredients / nutritional ingredients, is high in utilization rate of ganoderma lucidum, can improve the health level of intestinal tracts, promotes absorption and utilization of nutrient substances by the intestinal tracts, and provides a fundamental material guarantee for improving the immunity of the organism; immune cells are activated, and related lymphatic tissues of intestinal tracts are stimulated, so that the effects of regulating cellular immunity and humoral immunity levels are achieved; the preparation method provided by the invention does not use high-temperature, high-pressure and toxic and harmful reagents, has mild conditions, and is simple, easy to operate and environment-friendly; no toxic and harmful substances are generated, and generated filter residues can be directly used as animal feed or plant fertilizer; the prepared ganoderma lucidum peptide is small in molecule, high in activity, rich in active substance variety, high in yield and easy to absorb and utilize by organisms.

The invention relates to the technical field of bioengineering, in particular to a preparation process for extracting antihypertensive peptide from euphausia superba. The process comprises the following steps: adding a ferulic acid / arginine double-grafted pullulan compound in a preparation process, and breaking an emulsification interface by utilizing beta-cyclodextrin adsorption and phospholipase A2 cracking to obtain a soluble pretreatment product; meanwhile, short-time round-trip pH pulse is implemented in the incision enzyme hydrolysis process to activate potential enzyme cutting sites, finally, hydrophobic aggregation peptides are removed through weak acid rapid elution, and excision hydrolysis is performed to obtain a target product with the molecular weight of 0.5-3 kDa, so that dynamic induction and antioxidant steady-state dual control of hydrolysis reaction is realized; the generation proportion of short peptides and the ACE inhibition persistence are improved, and the flavor and the storage stability are remarkably improved. The method is controllable in technological process, high in repeatability and suitable for large-scale preparation of the euphausia superba protein functional peptide and development and application of antihypertensive food.

The application discloses a preparation method of egg yolk phospholipid polypeptide which is helpful for bone growth and improvement of brain health, and comprises the following steps: (1) raw material pretreatment to obtain a reaction substrate solution; (2) primary enzymolysis to prepare a primary enzymolysis solution; (3) secondary enzymolysis to prepare a secondary enzymolysis solution; (4) inactivation, the temperature of the secondary enzymolysis solution prepared in the step (3) is adjusted to 85-95 DEG C, and the enzyme is completely inactivated by keeping for 10-15 min; (5) fermentation; (6) post-treatment, the fermentation liquid after the fermentation is kept at 50-60 DEG C for 10-30 min, and then cooling, centrifugation, collection of supernatant, vacuum freeze drying or spray drying of the supernatant are carried out, so that the egg yolk phospholipid polypeptide powder is obtained. The natural compound rich in specific functional peptide segments and phospholipids is prepared through the two-step enzymolysis coupling fermentation process, can effectively promote the growth and development of the bone, significantly improve the learning, memory and cognitive ability, and can significantly promote the proliferation of osteoblasts.

The invention belongs to the technical field of biological medicines, and particularly relates to a multifunctional peptide and application thereof in anti-inflammatory, antibacterial, mucous membrane repair and intestinal secretion promotion. Specifically, the invention designs a polypeptide and an analogue which have guanylate cyclase C receptor (GUCY2C) agonist activity and anti-inflammatory, antibacterial and mucous membrane repair functions at the same time. The polypeptide can be combined with GUCY2C, stimulate generation of cyclic guanosine monophosphate (cGMP) in cells, promote intestinal fluid secretion, inhibit release of proinflammatory factors, relieve inflammatory response, promote repair of intestinal injury mucosa, inhibit growth of opportunistic pathogenic bacteria and accelerate intestinal homeostasis recovery after intestinal preparation. The composition can effectively promote intestinal tract cleaning, does not cause intestinal inflammation and mucosal lesion, has no hemolytic toxicity and cytotoxicity, is high in safety, and has a good clinical application prospect.

The invention discloses pea peptide for enhancing immunity as well as a preparation method and application thereof, and belongs to the technical field of functional peptide preparation. The pea peptide prepared by adopting the pea peptide preparation method provided by the invention is high in safety and strong in activity, can be easily absorbed and utilized by an organism, meanwhile, abundant mineral substances, vitamins and other nutritional ingredients in the pea pods are greatly reserved, and experiments prove that the prepared pea peptide has good effects of enhancing the immunity and keeping the immunity balance of the organism; tender pea pods are creatively used as raw materials, the cost is low, the operation is simple and easy, no toxic and harmful substances are generated, the by-product is only the pod residues with very small particle sizes, the method can be directly used for preparing animal feed or fertilizers for growth of plants such as peas, the method is environment-friendly, and the comprehensive utilization rate of the raw materials can be increased.

The invention discloses an application of a CD177 targeted membrane modified liposome in preparation of a medicine for preventing and / or treating psoriasis. The structure of the CD177 targeting membrane modified liposome comprises a PADI4 inhibitor loaded liposome, a targeting recognition layer formed by coupling CD177 targeting peptide on the surface of the liposome, and a bionic functional layer formed by coating a natural neutrophile granulocyte membrane on the outermost layer. Based on the core pathological mechanism of 'CD177 + neutrophil-NETs' of psoriasis, the inflammation chemotaxis / cell factor neutralization function of a neutrophil membrane, the precise targeting function of CD177 peptide and the NETs inhibition function of a PADI4 inhibitor are creatively and organically integrated, and the constructed GNLC achieves the synergistic treatment effect of 'targeting enrichment-mechanism blocking-microenvironment remodeling'; meanwhile, excellent stability and safety are achieved, and a new precise treatment strategy far better than that in the prior art is provided for psoriasis.

This invention discloses a highly efficient serine protease for degrading α-keratin, its degradation method, and its applications. The serine protease is named PliKerS, and its full-length amino acid sequence is shown in SEQ ID No. 2, with the corresponding coding gene sequence shown in SEQ ID No. 1. The amino acid sequence of the recombinant serine protease rPliKerS, which has its N-terminal signal peptide removed and a purification tag attached to its C-terminus, is shown in SEQ ID No. 4, with the corresponding coding gene sequence shown in SEQ ID No. 3. The recombinant serine protease rPliKerS degrades α-keratin under mild conditions, avoiding the environmental burden of strong alkaline treatment. It is particularly suitable for applications involving pet hair, hair waste, and leather pretreatment. The degradation products are soluble proteins and peptides, which can be used as high-value-added amino acid raw materials, functional peptide raw materials, biodegradable materials, bio-based material precursors, bio-feed proteins, fertilizers, etc., exhibiting high resource utilization value and promising industrial application prospects.

Described herein is a bifunctional peptide, compositions comprising the same, and methods useful for treatment of peri-implant disease.

The invention discloses a protease cleavable liposome and application thereof in treating colon cancer. The preparation method of the protease cleavable liposome comprises the following steps: synthesizing functional peptide from cancer homing peptide, protease cleavable protein fragments and lysine; the preparation method comprises the following steps: reacting a functional peptide with difunctional polyethylene glycol COOH-PEG2000-Mal, and covalently linking the obtained product with DSPE-NHS to obtain a protease cleavable material; and coupling the aPDL1 antibody to a protease cleavable material to obtain the protease cleavable liposome. The protease cleavable liposome can activate cytokines to kill tumor cells, so that effective endocytosis by colon cancer cells is realized, and an immune checkpoint blocking effect is achieved. Therefore, the protease cleavable liposome not only can be used as an effective delivery carrier for immune checkpoint blocking treatment, but also can be used as a universal platform for colon cancer immunochemistry combined treatment. The method has an important application value.

The application relates to the field of biotechnology, and discloses a method and system for regulating the ratio of amino acids and peptides after soybean proteolysis, which comprises collecting enzyme-coupling parameters in the whole proteolysis process to generate time series data, obtaining a multi-dimensional dynamic covariance matrix based on the data, identifying significant enzyme interaction through the matrix, and generating a list; a multi-level enzyme interaction network model is constructed according to the list, and finally, dynamic and accurate regulation of the ratio of amino acids and peptides is realized based on the model; the application can effectively solve the identification problem of hidden interaction interference in a multi-enzyme system, improve the consistency and predictability of product functions, and reduce the batch-to-batch fluctuation of the content of target functional peptides.

The invention relates to the technical field of health-care food, and discloses a composition and method for improving storage stability of donkey-hide gelatin peptide tablets, and the method comprises the following steps: firstly, treating donkey-hide gelatin by using acid protease and neutral protease in sequence through a sequential enzymolysis method to prepare composite donkey-hide gelatin peptide composed of grabbing peptide and stable peptide; then, the composite donkey-hide gelatin peptide and metal salt are subjected to a synergistic chelation reaction, and a donkey-hide gelatin peptide metal complex is obtained; and finally, adding sodium alginate into the complex solution, constructing a three-dimensional gel network in situ by using metal ions in the complex as a cross-linking agent, and drying, granulating and tabletting to obtain the final tablet. According to the method, the bifunctional peptide system prepared by sequential enzymolysis obviously improves the complexing rate of metal ions and inhibits oxidation reaction caused by free ions; meanwhile, the in-situ constructed gel network structure is used as a physical skeleton, so that the friability and hygroscopicity of the tablet are effectively reduced.

The invention discloses an extracellular matrix polypeptide as well as a preparation method and application thereof. The invention provides an extracellular matrix polypeptide composition, the weight-average molecular weight of a polypeptide component in the extracellular matrix polypeptide composition does not exceed 2500Da, and the molecular weight distribution index of the polypeptide component is greater than 1.5. The extracellular matrix polypeptide provided by the invention gives consideration to reasonable control of molecular weight distribution and retention of functional peptide fragment components, has the effects of promoting cell proliferation, promoting cell antioxidation and moisturizing, relieving and the like, and is wide in application.

This invention belongs to the field of food biotechnology and bioactive peptides, and specifically relates to a cottonseed protein-derived ACE-inhibiting peptide, its screening, modification methods, and applications. The screening method of this application includes four core steps: virtual enzymatic library construction, multi-stage computer filtering, molecular docking screening, and in vitro activity verification, obtaining 194 high-confidence candidate peptides. Four cottonseed protein-derived peptides (SYF, DIF, IAF, and RFY) with in vitro ACE-inhibiting activity were verified, all exhibiting mixed ACE-inhibiting activity, with DIF showing the strongest inhibitory activity. Molecular dynamics simulations confirmed the structural stability of the ACE-DIF complex. A modified peptide FIF was obtained through a dipeptide module substitution strategy, with an IC50 value of [missing information]. 50 The activity was significantly reduced, approximately 3.7 times that of the parent peptide DIF, and the inhibition type changed from mixed to competitive. This invention provides a new approach for the development of natural antihypertensive functional peptides and the high-value utilization of plant protein resources.

The present application relates to the application of a milk-derived functional peptide LSRYPSYG, in particular, the present application provides a milk-derived functional peptide comprising a polypeptide of sequence LSRYPSYG as shown in SEQ ID NO: 1, and the milk-derived functional peptide has anti-wrinkle and soothing functions.

The application relates to the technical field of coating surface preparation, in particular to an anticoagulant coating based on a novel tick-derived anticoagulant protein Qinghaienin and a preparation method thereof. 167‑192 Wherein the amino acid sequence of the functional peptide segment is shown in SEQ ID NO. 2. The anticoagulant coating is prepared by using a polydopamine coating as a base layer, and then covalently grafting the anticoagulant protein Qinghaienin or the functional peptide segment Qinghaienin 167‑192 which can inhibit the activation of the FXII factor is covalently grafted through an amide bond to the surface of a medical polymer material, and a novel anticoagulant coating is successfully constructed. Compared with unmodified medical polymer materials, the modified coating can significantly enhance the hydrophilicity of the material, inhibit platelet adhesion and activation, effectively prolong the blood clotting time in vitro, and exhibit excellent anticoagulant performance.

The invention provides a perfluorinated modified and integrated multifunctional peptide bPEI derivative delivery carrier, a carrier / pDNA compound and a preparation method thereof, and belongs to the technical field of gene delivery systems. The preparation method comprises the following steps: S1, dissolving bPEI in methanol, adding pentafluoropropionic anhydride, carrying out a first reaction, and dialyzing the obtained reaction liquid to obtain PF; s2, dissolving DBCO-ss-COOH in DMF (Dimethyl Formamide), adding EDC.HCl and NHS (N-Hydroxysuccinimide), and carrying out second reaction, so as to obtain a reaction solution after carboxyl activation; adding the carboxyl-activated reaction solution into a PF solution, carrying out a third reaction, and dialyzing the obtained reaction solution to obtain PF-DBCO; and S3, adding polypeptide RGD-NLS (N3)-TAT into the PF-DBCO solution, carrying out a fourth reaction, and dialyzing the obtained reaction solution to obtain the PF-RNT. Through double design of perfluorinated modification and RGD-TAT-NLS polypeptide functionalization, the problems of endosome retention, insufficient nuclear localization, relatively high toxicity and the like of traditional bPEI are solved in the continuous and efficient delivery process of transmembrane transport, nuclear localization and transcription expression, so that excellent balance is achieved between transfection efficiency and biological safety.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to an osteoarthritis targeted traditional Chinese medicine nano preparation and a preparation method thereof.The traditional Chinese medicine nano preparation is prepared from, by weight, 15-25 parts of traditional Chinese medicine extract, 4-8 parts of functional peptide, 3 parts of nano zinc oxide, 12-15 parts of coupling agent, 10 parts of modifier, 30-48 parts of phospholipid and 14 parts of cholesterol; the traditional Chinese medicine extract obtained through toxicity attenuation and purification contains rich terpenoids, flavones, saponins and other micromolecular anti-inflammatory immune regulation active substances, multi-target treatment of arthritis is provided, the designed functional polypeptide has inflammation specific response and cell penetrating capacity, focus enrichment of treatment components is achieved, and the treatment effect is good. The toxicity to normal tissues is reduced while the curative effect is improved.

The application discloses a kind of alpha-lactalbumin multifunctional peptides with antioxidant, anticoagulant and tyrosinase inhibitory activity and application thereof, and belongs to the field of food, biopharmaceutical and cosmetic technology.The multifunctional peptide SEKLDQWLCEKL is screened, and the DPPH clearance rate and ABTS clearance rate of the multifunctional peptide SEKLDQWLCEKL are highest, which can reach 62.32% and 88.32% respectively, the clearance rate of hydroxyl radical is highest, which can reach 78.89%, PT and TT are respectively extended by 3.4 s and 4.7 s, and the inhibition rate of tyrosinase is highest, which can reach 89.07%, which overcomes the limitation of existing single functional peptide in application.

The invention discloses a compound enzyme enzymolysis process of a suckling pig liver active peptide as well as a product and application of the suckling pig liver active peptide, and belongs to the technical field of extraction and preparation of functional peptides. The technical problems to be solved are that an adaptive compound enzyme formula is lacked in the existing preparation of the active peptide of the suckling pig liver, the enzymolysis efficiency of a single enzyme or a conventional compound enzyme is low, active peptide components in the suckling pig liver cannot be efficiently released, and the activity is not ideal. In the research and development of bioactive peptides for hepatitis and liver injury, no bioactive peptide from suckling pig liver is efficiently prepared and has a definite liver protection function, and the existing liver protection bioactive peptide has the problems of unstable curative effect and the like. According to the technical scheme, the compound enzyme enzymolysis process is characterized in that compound enzyme is used, and the compound enzyme is composed of neutral protease, papain, trypsin and lipase.

The application discloses a kind of immunity-enhancing pea peptides and its preparation method and application, belong to functional peptide preparation technical field.Preparation method includes: taking pea protein preheating, adding alkaline protease, flavour protease and papain complex protease compound to carry out first-stage enzymolysis, after enzyme inactivation, amino peptidase is added to carry out second-stage directional enzymolysis, and the obtained enzymatic hydrolysate is collected by membrane separation component with molecular weight less than 1000u, and is concentrated and dried to obtain.The application adopts endo-enzyme combined with exo-enzyme two-stage step enzymolysis strategy, and the prepared pea peptide molecular weight is less than 1000u Peptide segment ratio >80%, and the peptide content is >90%, without bitter taste, and has significant immune-enhancing activity, and can be widely applied in food, health food or pharmaceutical field.

The present invention relates to a bifunctional peptide comprising a virus-binding portion (2) and a mucin-binding portion (1) covalently linked to the virus-binding portion (2). The virus-binding portion is selected from peptides that bind to SARS-CoV-2, influenza A virus, influenza B virus, rhinovirus and other enteroviruses, human parainfluenza virus, and / or metapneuvirus. The mucin-binding portion is selected from lectins such as trefoil factor 3.

The application discloses a preparation method and application of a Tau-derived minimum functional peptide targeting a CUL3-RhoA axis, and an amino acid sequence of the Tau-derived minimum functional peptide is shown as SEQ ID NO. 4: GNIHHKPGGGQVEVKSEKLD. The application of the Tau-derived minimum functional peptide targeting the CUL3-RhoA axis in the preparation of a drug for treating diseases related to over-activation of osteoclasts belongs to the technical field of biological medicines, and the drug plays a pharmacological effect by blocking RhoA ubiquitination through CUL3, activating a RhoA-ROCK-MLC2 tension axis, and destroying a closed loop of osteoclasts. The Tau-derived minimum functional peptide can avoid application defects of full-length proteins, has low toxicity, high selectivity and physiological friendliness.

The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.

The application provides a red kidney bean functional peptide, a red kidney bean active peptide and a preparation method and application thereof, and belongs to the fields of biological medicine and food technology. The red kidney bean functional peptide comprises at least one of the following polypeptides: peptide 1, peptide 2 and peptide 3; the amino acid sequence of the peptide 1 is LPLF; the amino acid sequence of the peptide 2 is FLS; and the amino acid sequence of the peptide 3 is WYG. The application provides a preparation method of the red kidney bean active peptide, comprising the following steps: after broken red kidney beans are treated with alkali, the broken red kidney beans are subjected to first enzymolysis by using cellulase and saccharifying enzyme, and then subjected to second enzymolysis by using alkaline protease; and a component with a molecular weight of 3-10 kDa is collected to obtain the red kidney bean active peptide. The red kidney bean functional peptide and the red kidney bean active peptide can regulate blood lipids, regulate blood sugar and relieve oxidative stress, and have a good application prospect in the fields of functional food or medicine.

The invention belongs to the field of biomedical functional peptides and healthy foods, and particularly relates to a sea cucumber peptide for repairing gastrointestinal mucosal injury and a preparation method thereof. The sea cucumber peptide is prepared from sea cucumber oligopeptide, 2-hydroxy-4-methoxyphenylacetamide, beta-cyclodextrin, glycerol, potassium sorbate and deionized water, wherein the sea cucumber oligopeptide is modified by a glycyrrhizic acid-amino acid deep eutectic system. In the modification process, a deep eutectic system is formed through monosodium glycyrrhizinate and L-glutamic acid, and the sea cucumber oligopeptide is subjected to hydrogen bond recombination and amidation reaction, so that the structural stability and bioavailability of the sea cucumber oligopeptide in an acid environment are improved from the molecular level; the 2-hydroxy-4-methoxyphenylacetamide is used as a phenylacetamide organic small molecule with a simple structure, and can be used for cooperatively regulating the energy metabolism of cells and promoting the regeneration of epithelial cells of mucous membranes. The preparation method is mild and controllable, and the product is safe and stable, and can be widely applied to the fields of gastrointestinal repair preparations and functional foods.