37 results about "Artificial protein" patented technology

It is intended to provide a peptide or a phage recognizing nanographite structures and thus enabling efficient recognition, binding, separation and alignment of nanographite structures such as carbon nanohoms or carbon nanotubes, an artificial protein or a chimeric molecule comprising the above-described peptide bonded to a functional peptide, a protein, a labeling, etc., and a complex of the above-described peptide molecule, artificial protein or chimeric molecule with a nanographite structure. By panning peptide-presenting phages bonded to nanographite structures, a nanographite structure-binding peptide capable of specifically recognizing nanographite structures such as carbon nanohoms or carbon nanotubes is obtained.

The invention relates to artificial modified proteins, preferably fusion proteins, having a reduced immunogenicity compared to the parent non-modified molecule when exposed to a species in vivo. The invention relates, above all, to novel immunoglobulin fusion proteins which essentially consist of an immunoglobulin molecule or a fragment thereof covalently fused via its C-terminus to the N-terminus of a biologically active non-immunoglobulin molecule, preferably a polypeptide or protein or a biologically active fragment thereof. In a specific embodiment, the invention relates to fusion proteins consisting of an Fc portion of an antibody which is fused as mentioned to the non-immunological target molecule which elicits biological or pharmacological efficacy. The molecules of the invention have amino acid sequences which are altered in one or more amino acid residue positions but have in principal the same biological activity as compared with the non-altered molecules. The changes are made in regions of the molecules which are identified as T-cell epitopes, which contribute to an immune reaction in a living host. Thus, the invention also relates to a novel method of making such fusion proteins by identifying said epitopes comprising calculation of T-cell epitope values for MHC Class II molecule binding sites in a peptide by computer-aided methods.

The invention relates to an ecological breeding method for honeysuckle flower chicken. The method comprises a heat preservation eliminating stage, a feed training stage and a stocking stage, wherein at the heat preservation eliminating stage, 5kg of insect protein additive and 1kg of Chinese medicinal epidemic prevention additive are added into 100kg of basal feed; at the feed training stage, adding 1-1.5kg of insect protein additive and 1.5kg of Chinese medicinal epidemic prevention additive into 100kg of basal feed; at the stocking stage, adding 1-1.5kg of insect protein additive and 2kg of Chinese medicinal epidemic prevention additive into 100kg of basal feed; at the stocking stage, 1-1.5kg of insect protein additive, 2kg of Chinese medicinal epidemic prevention additive and 1kg of meat quality improving additive are added into 100kg of basal feed two months before marketing in order to improve the meat quality of the honeysuckle flower chicken. According to the ecological breeding method for honeysuckle flower chicken, hormones, antibiotics and artificial proteins are not fed, and a stocking way is adopted, so that the obtained honeysuckle flower chicken have the advantages of good mouthfeel, compact meat quality and abundant nutrients.

The invention discloses a preparing method of flame-proof artificial protein hair, which comprises the following steps: (1)dissolving superfine hair powder and acrylonitrile copolymer in solvent at 55-75 deg. c; stirring and mixing for 2-5 h; filter-pressing; debubbling; getting mixed spinning raw liquid; (2)choosing water solution of 45-75% dimethyl formamide (DMF)or dimethyl acetamide (DMAC)as setting bath; spinning; freezing; washing; drawing; hot-shaping; getting the product. This method possesses simple operation, wide source of raw material and low cost, which product possesses good flame-proof property.

The invention discloses an insect-resistant fusion gene and an encoded protein as well as application thereof. The gene comprises a nucleotide sequence of encoding chitin binding protein and a nucleotide sequence of encoding Vip3 toxin in turn from 5' to 3'. The two nucleotide sequences are located in one opening reading frame. Compared with original chitin binding protein and by Vip3 protein, the artificial protein molecule formed by fusing one chitin binding protein and one Vip3 toxin designed by the invention has the advantages that the insecticidal spectrum is wide, various important pests (such as beet armyworm, armyworm and mealywing) in lepidoptera and hemiptera can be simultaneously prevented and controlled and the insecticidal efficiency reaches up to 50%-100%; the combined utilization of the proteins with different functions and other insect-resisting proteins (such as ICPs) is benefited, the insecticidal spectrum is further widened, and the generation of pest resistance is delayed.

The invention provides artificial protein having vibrio cholerae toxin A-subunit and staphylococcus aureus characteristics and an application thereof, which belong to the technical field of protein engineering. According to the invention, the artificially designed vibrio cholerae toxin A-subunit and staphylococcus aureus DNA sequences are subjected to synthesis, an amino acid sequence is shown as SEQ ID NO.1, and a nucleotide sequence is shown as SEQ ID NO.2. The fusion protein has the advantages of good immunogenicity and strong specificity, can effectively introduce into an biological body through a mucous membrane, and a neutralizing antibody is generated in the body, the neutralizing antibody can be used for preparing a biological product as an immunologic adjuvant, and the market value is obvious.

The present invention provides a peptide sequence, a phage, an artificial protein or a chimeric molecule having a binding ability to titanium, silver, silicon, necessary to confer higher capacity of titanium, silver, silicon material with the use of soft matters, or to provide a complex of a peptide, a phage, an artificial protein or a chimeric molecule, and titanium, having the peptide sequence and functional peptide sequence. By bringing into contact a population of phage wherein said phage of said population collectively express a library of different peptide sequence, recovering titanium bound to phage particles via peptide sequence by centrifugation, proliferating the obtained phage particles in bacteria, and repeating panning operation and concentrating titanium binding phage clones. Among the phage clones, peptide RKLPDAPGMHTW and the like is identified. As for a peptide having a binding ability to titanium, silver, silicon, RKLPDA or RALPDA can be exemplified.

The present invention provides a peptide sequence, a phage, an artificial protein or a chimeric molecule having a binding ability to titanium, silver, silicon, necessary to confer higher capacity of titanium, silver, silicon material with the use of soft matters, or to provide a complex of a peptide, a phage, an artificial protein or a chimeric molecule, and titanium, having the peptide sequence and functional peptide sequence. By bringing into contact a population of phage wherein said phage of said population collectively express a library of different peptide sequence, recovering titanium bound to phage particles via peptide sequence by centrifugation, proliferating the obtained phage particles in bacteria, and repeating panning operation and concentrating titanium binding phage clones. Among the phage clones, peptide RKLPDAPGMHTW (SEQ ID NO: 3) and the like is identified. As for a peptide having a binding ability to titanium, silver, silicon, RKLPDA (SEQ ID NO: 1) or RALPDA (SEQ ID NO: 2) can be exemplified.

The invention discloses an insecticidal fusion protein which comprises IPD072 protein and Vip3 protein. Compared with the previous IPD072 protein and Vip3 protein, the designed artificial protein molecule formed by fusing IPD072 protein and Vip3 toxin has the following advantages: the insecticidal spectrum is broad, multiple important pests (such as laphygma exigua, armyworm and diabrotica virgifera) of lepidoptera and coleoptera can be controlled at the same time, and the insecticidal efficiency is as high as 50-100%; and moreover, the joint utilization of proteins with different functions and other insect-resistant proteins (such as ICPs) is promoted, the insecticidal spectrum is further expanded, and the generation of insect resistance is delayed.

The invention discloses an insect-resistant protein, an insect-resistant fusion protein, a coding gene, a carrier and application. According to the invention, the insect-resistant protein has an amino acid sequence with 90% of homology compared with SEQ ID NO: 1; and the insect-resistant fusion protein, from an N end to a C end, successively contains a BT crystal toxin Cry1 protein and an insect-resistant protein Cry2Aj. The invention first discloses the BT crystal toxin Cry2Aj protein with high insecticidal ability, and designs an artificial protein molecule which is prepared by fusing a Cry1Ab crystal toxin and the crystal toxin Cry2Aj protein; and compared with an original physical mixture of Cry1 and Cry2 crystal toxins, the artificial protein molecule has the following advantages: insecticidal activity is increased by more than 10 times, and insecticidal spectrum is wider; the insecticidal protein provided by the invention can kill main lepidopterous pests like cotton bollworms, beet armyworms, corn borer and Chilo suppressalis of rice, corn and cotton; moreover, the fusion protein disclosed in the invention can effectively retard occurrence of insect resistance.

Protein engineering is an emerging area that has expanded the understanding in the art of protein folding and laid the groundwork for the creation of unprecedented structures with unique functions. The first native-like pore-forming protein, small globular protein (SGP), has previously been designed. It has now been discovered that this artificially engineered protein, and analogs and homologs thereof, have membrane-disrupting properties and anti-tumor activity in several cancer animal models. A mechanism for the selectivity of SGP toward cell membranes in tumors is proposed and validated herein, thereby confirming the proposed mechanism of action. Thus, SGP is established herein as the prototype for a new class of artificial proteins designed for therapeutic applications.

The present invention provides proteins having one or more similarities to the artificial protein Top7 or to a Top7 derivative. Proteins of the invention have one or more loops that are longer than the corresponding loops of Top7, and / or that bind to a preselected target molecule. The invention also provides nucleic acids and cells useful in producing the proteins and methods for their use.

The invention discloses a preparation method of an anti-adhesive membrane used after neurosurgical operations. The method comprises the following steps: dissolving polylactide, artificial protein and pure natural collagen in an organic solvent to obtain a macromolecular solution; conducting electrostatic spinning treatment to the macromolecular solution to obtain an electrostatic spinning film, wherein the voltage of electrostatic spinning is 25.2KV and a receiving distance is 21cm; and drying the electrostatic spinning film in a drying oven for 3-3.5h at 50-55 DEG C to obtain the anti-adhesive membrane. The anti-adhesive membrane prepared by the preparation method disclosed by the invention is simple to prepare, good in ductility and good in anti-adhesion capacity.

The invention provides a method for improving the half life of a hydrophilic substance. The method comprises connecting the hydrophilic substance to a self-assembled polypeptide, wherein and the connection site is located in a non-interference region of the active site of the hydrophilic substance. According to the invention, the self-assembled polypeptide is connected with the non-interfering region of the active site of the hydrophilic substance, and the formed artificial fusion protein can form nano particles under the self-assembly mediation of the self-assembled polypeptide; in addition,compared with the hydrophilic substance, the nano particles have greatly improved stability, pharmacokinetics and biodistribution in an organism, and the half life is obviously improved.

The invention discloses a high-strength adhesive for treating fracture. The adhesive comprises 100 to 300 milligrams of artificial protein A, 200 to 400 milligrams of protocollagen, 50 to 200 milligrams of biological zymogen, 100 to 500 milligrams of biological active calcium, 100 to 500 milligrams of polyglycolic acid, 100 to 400 milligrams of polylactic acid, and 10 to 50 ml of distilled water. A bone repair material of the invention has high biocompatibility and bone conduction property and can also be used for carrying out internal fixation of fracture and treatment for bone defect reconstruction in an injection mode.

The purpose of the invention is to provide a material for tissue regeneration, which suppresses bacterial growth and accelerates granulation tissue formation or epithelialization. The invention is a material for tissue regeneration comprising an artificial protein polymer (A), wherein (A) has a polypeptide chain (Y), in which 2 to 200 amino acid sequences (X) of any one type of a VPGVG sequence (2), a GVGVP sequence (3), a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (4) are continuous, and / or a polypeptide chain (Y') obtained by substituting 1 to 100 amino acids in (Y) with lysine and / or arginine, and the total number of (Y) chains and (Y') chains in (A) is 1 to 100, and the hydrophobicity of (A) is 0.2 to 1.2.

The invention discloses a protein compound based on colicin E family DNA enzyme and application of the protein compound in an artificial protein scaffold. The protein complex comprises any one or more of an interaction pair formed by a CL2 protein and an Im2 protein, an interaction pair formed by a CL7 protein and an Im7 protein, an interaction pair formed by a CL8 protein and an Im8 protein, and an interaction pair formed by a CL9 protein and an Im9 protein. According to the invention, protein engineering transformation is carried out on carboxyl terminal DNase structural domains of DNA enzymes CE2, CE7, CE8 and CE9 of a CE family to obtain a mutant which loses DNA enzyme activity but still retains ultrahigh affinity with a corresponding immune protein Im, and CL2 / Im2, CL7 / Im7, CL8 / Im8 and CL9 / Im9 protein interaction pairs are constructed. Research finds that the protein interaction pairs have the properties of heat resistance, high affinity, high specificity, small molecular weight, high assembly speed and the like, an artificial protein scaffold is constructed based on the protein interaction pairs, and a novel platform is established for construction of an artificial multi-enzyme compound.

Provided is an artificial protein that is present in a folded part of the secondary structure in a structural region having the antigen-binding activity of a protein having at least an antigen-bindingregion of an antibody and that is fused with a physiologically active peptide at a site exposed on the protein surface.

The invention provides an artificial protein for quantitative analysis of the proteome of a sample, cell or organism, comprising at least two consecutive peptides linked by a cleavage sequence for separating the peptides; a singular marker on one or more peptide for determination of the absolute amount of this fragment; and N-terminal and C-terminal extensions for protection of the peptides; wherein each peptide represents one single protein of the sample, cell or organism and each peptide is in a defined stoichiometry. The invention further provides a collection of peptides, a vector and a kit comprising the artificial protein and a method for quantitative analysis of the proteome.

The invention discloses a preparation method of a micron-sized ultrathin metal sheet for a conductive adhesive, and relates to the field of electronic components. The preparation method comprises the following steps that step 1, material preparation is carried out, 45-68 parts of polyvinyl formal, 20-30 parts of artificial protein powder colloid and 10-15 parts of zinc oxide are blended according to a proportion through an electronic scale; and step 2, stirring is carried out, the raw materials blended according to the proportion are poured into a stirrer, and stirring is carried out for 35 minutes under the condition that the rotating speed is 120-150 revolutions per minute. By arranging a spraying mechanism, a stripping machine and a stirring machine, the tedious steps of traditional manual operation are changed, so that the method for manufacturing the micron-sized ultrathin metal sheet for the conductive adhesive is simpler, the working efficiency is higher and higher, the investment of human capital is saved, and the production quality of products is improved; and meanwhile, the zinc oxide is added as one of the raw materials, so that the wear resistance and durability of the micron-sized ultrathin metal sheet for the conductive adhesive are improved.

The present invention provides proteins having one or more similarities to the artificial protein Top7 or to a Top7 derivative. Proteins of the invention have one or more loops that are longer than the corresponding loops of Top7, and / or that bind to a preselected target molecule. The invention also provides nucleic acids and cells useful in producing the proteins and methods for their use.

Nanodiamonds having a positive ξ-potential of at least 1 mV for use in sequestration of at least one FGF family member in organisms in vivo and in vitro. It has been found that nanodiamonds with a positive ξ-potential show an extremely strong and selective binding to FGF family members, thus leading to their usability in the treatment of diseases related to aberrant FGF-FGFR signalling and / or interaction.

The invention provides artificial protein having vibrio cholerae toxin A-subunit and staphylococcus aureus characteristics and an application thereof, which belong to the technical field of protein engineering. According to the invention, the artificially designed vibrio cholerae toxin A-subunit and staphylococcus aureus DNA sequences are subjected to synthesis, an amino acid sequence is shown as SEQ ID NO.1, and a nucleotide sequence is shown as SEQ ID NO.2. The fusion protein has the advantages of good immunogenicity and strong specificity, can effectively introduce into an biological body through a mucous membrane, and a neutralizing antibody is generated in the body, the neutralizing antibody can be used for preparing a biological product as an immunologic adjuvant, and the market value is obvious.

The invention discloses an insect-resistant fusion gene and an encoded protein as well as application thereof. The gene comprises a nucleotide sequence of encoding chitin binding protein and a nucleotide sequence of encoding Vip3 toxin in turn from 5' to 3'. The two nucleotide sequences are located in one opening reading frame. Compared with original chitin binding protein and by Vip3 protein, the artificial protein molecule formed by fusing one chitin binding protein and one Vip3 toxin designed by the invention has the advantages that the insecticidal spectrum is wide, various important pests (such as beet armyworm, armyworm and mealywing) in lepidoptera and hemiptera can be simultaneously prevented and controlled and the insecticidal efficiency reaches up to 50%-100%; the combined utilization of the proteins with different functions and other insect-resisting proteins (such as ICPs) is benefited, the insecticidal spectrum is further widened, and the generation of pest resistance is delayed.

The invention discloses a novel tire sealing composite material containing 601 tackifying resin and belongs to the technical field of tires. The formula is characterized by being composed of the 601 tackifying resin, epichlorohydrin rubber, a cross-linking agent BIPB (Bi(butylperoxyisopropyl)benzene), chlorinated polyethylene micro-powder, pine oil, crushed artificial protein fibers, a rubber antioxidant crotonaldehyde, epichlorohydrin rubber particles and epoxy soybean oil. The novel tire sealing composite material is mainly applied to a meridian vacuum tire and is the novel tire sealing composite material which can be used for rapidly self-repairing a notch (with the diameter smaller than or equal to 10mm), is not dried and frozen, is high in reliability and good in stability and is durable; the novel tire sealing composite material can be used in an environment at a temperature ranging from -60 DEG C to 108 DEG C for a long period. The product disclosed by the invention contains a plurality of types of macromolecular polymers; the state of the material in the tire is a sticky state with liquid and solid and can be stably and uniformly stuck on an inner wall of the tire for a long period, so that the dynamic balance of wheels is not influenced and a zero-leakage effect is also realized; compared with the prior art, the novel tire sealing composite material is definitely innovative improvement.

According to the invention, a functional module SyKerMb capable of efficiently degrading poultry feather waste to synthesize novel hemoprotein is artificially designed and created by utilizing a synthetic biological method. A recombinant vector of the functional module is constructed, and the recombinant vector is assembled and expressed in chassis microbial cell escherichia coli. Experiments prove that an engineering strain integrated with the transformation functional module has the biosynthesis capability of degrading animal waste feathers and efficiently synthesizing artificial hemoprotein, and can be applied to related industrial production of artificial protein preparations, artificial foods in the future and the like.

The invention belongs to the technical field of biology, and relates to a novel artificial protein targeting UBE2C and practical application of the novel artificial protein. The fusion protein disclosed by the invention is composed of two protein structural domains, namely a WHB structural domain and an NEL structural domain, wherein the WHB structural domain is derived from a structural domain of a natural APC2 protein which has direct interaction with UBE2C, and NEL is from a conserved E3 enzyme structural domain of an E3 enzyme IPAN9.8 of Shigella. The bio-PROTAC disclosed by the invention can be used for specifically recognizing UBE2C, successfully realizes ubiquitination modification of UBE2C protein in a cell-free environment, and can degrade UBE2C subjected to exogenous expression in cells.

The invention relates to an ecological breeding method for honeysuckle flower chicken. The method comprises a heat preservation eliminating stage, a feed training stage and a stocking stage, wherein at the heat preservation eliminating stage, 5kg of insect protein additive and 1kg of Chinese medicinal epidemic prevention additive are added into 100kg of basal feed; at the feed training stage, adding 1-1.5kg of insect protein additive and 1.5kg of Chinese medicinal epidemic prevention additive into 100kg of basal feed; at the stocking stage, adding 1-1.5kg of insect protein additive and 2kg of Chinese medicinal epidemic prevention additive into 100kg of basal feed; at the stocking stage, 1-1.5kg of insect protein additive, 2kg of Chinese medicinal epidemic prevention additive and 1kg of meat quality improving additive are added into 100kg of basal feed two months before marketing in order to improve the meat quality of the honeysuckle flower chicken. According to the ecological breeding method for honeysuckle flower chicken, hormones, antibiotics and artificial proteins are not fed, and a stocking way is adopted, so that the obtained honeysuckle flower chicken have the advantages of good mouthfeel, compact meat quality and abundant nutrients.

The invention discloses a fish feed for preventing and controlling the morbidity of fries for giant salamander breeding and a preparation method of the fish feed. The preparation method comprises thefollowing steps: cleaning radix sophorae flavescentis and artemisia argyi leaves, draining water, smashing the radix sophorae flavescentis and the artemisia argyi leaves into pulp, and centrifuging the pulp in a centrifuge at 200-300 r / min for 5-10 minutes to obtain supernatant; drying radix stemonae, fructus forsythiae, flos lonicerae and an additive, grinding the radix stemonae, the fructus forsythiae, the flos lonicerae and the additive into powder, placing vinasse into the powder, and carrying out fermenting for 5-7 days to obtain a fermented product; and mixing the supernatant, the fermented product, corn flour, sweet potato powder, a fish meal additive, a food binder and artificial protein, adding the mixture into deionized water, fully fusing the mixture in a stirrer, and preparingbait with the diameter of 0.05-0.1 mm in a granulator to obtain the product. The fish feed is prepared from medicinal materials, so that diseases of fishes are treated in a feeding process; and meanwhile, materials with large side effects such as antibiotics are not used, so that the fish feed has small side effects on fish schools and is free of residues.