32 results about "Protein particles" patented technology

The application discloses a Zanthoxylum bungeanum protein characteristic data-driven modeling method and system for functional expression, belongs to the technical field of food protein data modeling, obtains protein characteristic indexes, functional expression indexes and impurity interference indexes of Zanthoxylum bungeanum protein samples under different processing conditions, forms corresponding data and carries out standardization and abnormal data processing; based on the correlation degree of the protein characteristic indexes, the functional expression indexes and the impurity interference indexes, functional contribution factors and impurity domination factors are determined respectively, and comprehensive weights are generated; the protein characteristic data is weighted by using the comprehensive weights to obtain function-oriented protein characteristic data; then principal component extraction is carried out, and function-oriented principal components are screened according to the correlation between the principal components and the functional expression data and the impurity interference data; finally, a Zanthoxylum bungeanum protein functional expression prediction model is established; the application can weaken the influence of interference information such as pigments, turbidity, residual oil rate and non-protein particles on modeling, and improve the functional prediction stability.

The invention belongs to the technical field of protein, and aims to solve the problem that the application range of spider silk particles as a drug carrier is limited due to the fact that the spider silk particles are in negative net charge under a neutral condition and can only load drugs with positive charges or neutral charges at present. The novel recombinant spider silk protein is prepared on the basis of an N connection sequence of araneus ventricosus MaSp1, has positive net charge under a neutral pH value, can load a negatively charged drug, and expands the application range of the spider silk protein as a drug carrier; and a drug release test result shows that the recombinant spider silk protein is superior to MaSp2-based silk protein particles in the aspect of etoposide delivery.

The present disclosure relates to the generation of functionalised matrices comprising self- aggregating protein particles absorbed, combined or bound to the porous matrix. More particularly, the disclosure relates to matrices comprising p40 containing protein nanoparticles which modified matrices exhibit functional activity.

The invention relates to the technical field of food processing, in particular to high-protein low-fat walnut milk and a preparation method thereof. The raw materials comprise walnut kernels, defatted soybean powder, purified water, an emulsifier, a stabilizer and white granulated sugar. The method comprises the following steps: obtaining various raw materials for preparing the walnut milk, and pretreating walnut kernels and defatted soybean powder; grinding the pretreated walnut kernels to obtain walnut primary pulp, and mixing defatted soybean powder with water to obtain a soybean protein solution; mixing and blending the walnut puree and the soybean protein solution; carrying out two times of homogenization treatment on the prepared walnut milk coarse adjustment liquid to obtain walnut milk homogenized liquid; performing high-temperature sterilization on the homogenized walnut milk; through the mode, fat globules and protein particles in the walnut milk can be fully refined, the layering and precipitation phenomena easily occurring in the product storage process are effectively solved, the appearance quality of the product is greatly improved, and it is ensured that the walnut milk keeps uniform and stable texture in the whole shelf life.

The present invention relates to self-assembling multimeric protein particles displaying Borrelia antigens, namely an encapsulin protein particle displaying an 'outer surface protein A' (OspA) antigen and a DPS (DNA binding protein from starved cells) protein particle displaying a mutant 'complement regulator-acquiring surface protein 2' (CspZ) antigen for use in the vaccination against Lyme disease, particularly in combination.

The invention discloses a preparation method and application of a vegetable protein-pectin particle stable composite emulsion, and belongs to the technical field of vegetable protein emulsion preparation. The preparation method comprises the following steps: mixing thermally induced peanut protein particles with pectin, and carrying out high-speed shearing emulsification to construct the oil-in-water peanut protein-pectin composite emulsion. The composite emulsion disclosed by the invention is controllable and stable in structure and enhanced in function, and has a wide application prospect in the field of foods such as dairy products.

The application discloses a preparation method of a nitrite emulsion inhibitor and application thereof, and belongs to the technical field of food processing. The application prepares a double-protein particle solution by mixing wheat prolamin and sodium caseinate, then adds the double-protein particle solution into a mixed oil phase containing Chinese litse oil, and obtains a Pickering emulsion containing Chinese litse oil through a homogenization reaction. In addition, the prepared emulsion is added into meat paste, the lipid oxidation and spoilage of the meat paste are improved, the accumulation of nitrite in the meat paste is obviously inhibited during cold storage, the purposes of inhibiting bacteria and inhibiting the residue of nitrite in the meat paste during storage and improving the quality of the meat paste are realized, and finally high-quality meat paste with rich nutritional components and low nitrite residue is obtained. Moreover, the process provided by the application is simple, energy-saving and environment-friendly, and the large-scale production process is easy to implement, so the application can be widely promoted and has wide application prospects.

The invention discloses a technology for preparing micron-sized oleosin protein particles with high interfacial activity, and belongs to the technical field of grease protein processing. The method comprises two steps of oleosin protein self-assembly and protein particle size homogenization: mixing completely dried oleosin protein powder with an ethanol solution with the volume being 3-10 times of the mass of the protein powder, the concentration being 55%-75% and the pH value being 3-7 for 5-10 minutes, then treating for 30-90 seconds under ultrasonic waves of 200-500 W, standing the treated mixed solution for 2-3 hours at room temperature, and carrying out solid-liquid separation to obtain the oleosin protein particle size. And then centrifuging for 10-30 minutes at the speed of 3000-5000 rpm, collecting supernate obtained after centrifuging, and filtering for 1-3 times by using an organic filter membrane with the size of 3-5 microns to obtain filtrate which is a micron-sized oleosin protein particle solution with high interfacial activity. The particle size of the prepared high-interfacial-activity micron-sized oleosin protein particles is smaller than 2500 microns, and the interfacial tension can be effectively reduced when the high-interfacial-activity micron-sized oleosin protein particles are adsorbed to an oil-water interface.

The invention discloses a diaphragm and a preparation method thereof and a lithium-sulfur battery, the diaphragm comprises a base membrane and a cottonseed protein coating, the cottonseed protein coating is coated on at least part of the surface of the base membrane, the cottonseed protein coating comprises cottonseed protein particles, and the cottonseed protein particles are obtained by crosslinking cottonseed protein. The cottonseed protein coating is arranged on the surface of the diaphragm, so that the diaphragm has good mechanical performance and ionic conductivity, the shuttle effect of lithium polysulfide can be effectively inhibited, and the transmission performance of lithium ions is improved. When the diaphragm is applied to the lithium-sulfur battery, the diaphragm has higher mechanical strength, thermal stability and lithium polysulfide adsorption capacity, the cycling stability of the lithium-sulfur battery can be improved, and the overall performance of the lithium-sulfur battery can be improved.

The invention discloses an aerated beverage added with whey protein and a preparation method of the aerated beverage, and particularly, the aerated beverage comprises the following ingredients based on the total weight of the aerated beverage: 0.1-0.6% of concentrated whey protein powder, 1.5-7.5% of soluble soybean polysaccharide and 0.3-0.6% of sodium hexametaphosphate; the ingredients also comprise 2.0-4.0 times by volume of carbon dioxide; the mass fraction of protein in the aerated beverage is less than 0.5%. The ingredients of the aerated beverage added with whey protein can improve the repulsive force between protein particles and reduce particle aggregation, so that the stability of the aerated beverage is improved, and the color and luster of the product are improved.

The present invention relates to recombinant Modified Vaccina Virus Ankara (MVA) encoding Epstein-Barr virus (EBV) antigens, wherein surface glycoprotein 350 (EBV gp350) and glycoprotein gH (EBV gH) are fused to subunits of self-assembling multimeric protein particle PdhC (acetyltransferase of pyruvate dehydrogenase (PDH) complex) or DPS (DNA binding protein from starved cells).

The invention relates to an adenovirus vector purification medium as well as a preparation method and application thereof, the adenovirus vector purification medium comprises microspheres and a bionic polypeptide ligand, and the microspheres and the bionic polypeptide ligand are connected through an arm type branch chain; the bionic polypeptide ligand comprises any one or a combination of at least two of polypeptides with sequences shown as SEQ ID NO: 1-3. According to the invention, the bionic polypeptide ligand is connected to the microsphere through an arm type branch chain, so that the surface area of the microsphere is increased, and the defects of low protein adsorption load possibly caused by low specific surface area and the problems of poor flexibility, short spatial extension distance and steric hindrance effect when the polypeptide ligand is bound with macromolecular protein particles are overcome; the affinity and the purification efficiency of the adenovirus vector can be effectively improved.

The invention relates to a protein precipitation plate, a preparation method of the protein precipitation plate and raw materials for preparing the protein precipitation plate, the raw materials for preparing the protein precipitation plate comprise polytetrafluoroethylene resin and polyvinylidene fluoride resin, and the mass ratio of the polytetrafluoroethylene resin to the polyvinylidene fluoride resin is 1: 3-2: 1. The protein precipitation plate prepared from the raw materials not only can intercept precipitated protein particles without causing blockage, but also is high in flow velocity, does not adsorb to-be-detected targets (such as water-soluble vitamins, drug molecules and the like), and is superior to protein precipitation plates of imported brands in protein filtering efficiency and filtering time; and the pretreatment requirements of protein precipitation in biological analysis can be well met.

This invention provides a method for preparing micronized rice protein with lubricating properties. The method includes the following steps: S1: Pretreatment of sugar-refining rice residue, using sugar-refining rice residue as raw material, adding water to form a slurry, processing with a colloid mill, and then passing through a 60-mesh sieve to remove impurities; S2: Enzymatic desaccharification, adjusting the pH of the rice residue slurry in S1 to 7.0±0.5, and then adding 0.2% neutral amylase and 0.2% saccharifying enzyme. The method for preparing micronized rice protein with lubricating properties provided by this invention employs direct steam injection heating. By controlling the pressure and steam injection rate, rapid denaturation and coagulation of rice protein molecules are achieved, resulting in the production of micronized rice protein with uniform particle size and regular spherical shape. Furthermore, a two-stage homogenization technique is combined to further reduce particle size, effectively improving the solubility, surface activity, and emulsifying properties of the protein particles. The obtained micronized rice protein can be used as a fat substitute for the development of low-fat foods.

This disclosure provides protein-dried particles having a specified microstructure with beneficial properties. When combined with an aqueous solution, these dried particles form a macrocolloid with improved thickening and stabilizing properties. When combined with an oil, these dried particles form an oleogel with intrinsic oil-structuring and release properties. The microstructure is in the form of fibrils, sheets, or other particles that are substantially uninterconnected and have a high aspect ratio. These dried particles can be produced by denaturing a protein in an aqueous liquid and organizing the protein by freeze channel formation. The oleogel can be produced by gradually and gently adding a suitable oil so as not to pulverize the microstructure. When heated, the oleogel releases some, but not all, of the oil and forms an emulsion that is spreadable in an aqueous liquid and stable for at least 6 weeks. The oleogel can be used as a substitute for animal fats and tropical vegetable oils in food, cosmetics, and pharmaceuticals. TIFF2026509516000009.tif58170

The invention discloses a freeze-thaw enhanced blocky vegetable protein fat substitute with a double-network structure as well as a preparation method and application thereof, and belongs to the technical field of food science. The vegetable protein, the vegetable oil, the konjac polysaccharide and the xanthan gum are added according to a certain sequence, and are subjected to freezing-unfreezing circulation treatment to prepare the blocky fat substitute with a protein particle-polysaccharide chain-like double-network skeleton structure with relatively high gel strength. According to the fat substitute disclosed by the invention, the texture and taste of the product are remarkably improved; the enhanced cross-linked network structure effectively inhibits water loss, ice crystal growth and oil-water separation at low temperature, so that the emulsion gel keeps a stable structure in the freezing and unfreezing processes, fracture, layering and water-oil separation are avoided, and the characteristics of enhanced hardness and elasticity under freezing and thawing cycles are shown at the same time. The method has a great application prospect in replacement of animal fat in food.

This invention provides barley prolysin particles and their preparation method, as well as a method for preparing an oil-in-water emulsion, belonging to the field of food science and engineering technology. This invention employs proline endonuclease to enzymatically hydrolyze barley prolysin at a pH of 5.5-5.8, breaking it down into small-molecule barley prolysin polypeptides, thus reducing the size of the particles. Calcium ions in calcium chloride aqueous solution form coordination bonds with negatively charged groups such as carboxyl and hydroxyl groups on the barley prolysin polypeptide chain, regulating the charge distribution on the surface of the barley prolysin molecules and improving the dispersibility of the particles in water. Furthermore, the cycloalkenyl ether ketone active group in genipin molecules forms stable Schiff base covalent bonds with the free primary amino groups on the barley prolysin polypeptide chain, constructing a stable three-dimensional network structure and improving the thermal stability of the emulsion obtained from the barley prolysin particles.

The present application relates to the field of active substance embedding delivery technology, and particularly relates to a preparation method and application of corn protein particles for selectively embedding ginsenoside Rd. The preparation method comprises the following steps: (1) dissolving zein in an ethanol solution; (2) adding total ginsenoside to the zein solution; (3) preparing casein buffer, and quickly adding the casein buffer to the mixture; (4) removing ethanol by using a rotary evaporation method; (5) filtering the nanoparticle dispersion; and (6) freeze-drying. The prior art needs to use high-purity ginsenoside Rd monomers as embedding raw materials, and the direct embedding of crude extracts has no selectivity, and the product components are mixed and the quality is uncontrollable. The present application uses the specific binding ability of zein to ginsenoside Rd to selectively enrich ginsenoside Rd from a mixture containing multiple saponins, and the proportion of Rd in the embedded total saponins can reach more than 90%. The overall preparation cost is significantly reduced, and the production is suitable for large-scale industrialization.

The application provides a soybean protein isolate chopping process real-time monitoring method, comprising the following steps: in the chopping feeding stage, the initial moisture content of the soybean protein isolate powder is directly collected through a moisture probe, and the particle size distribution and the feeding amount of the current batch of protein powder are recorded to evaluate the surface wetting degree of the protein particles; according to the initial moisture content and the torque reading when the rotor starts, the matching degree of the surface wetting degree of the protein particles and the cutter head starting load is analyzed, the surface skin degree formed during the storage of the protein powder is evaluated, and the skin thickness grade is obtained; the skin thickness grade is compared with a preset grade comparison table, the monitoring time and the torque collection interval of the chopping hydration under the grade are obtained, and the hydration monitoring window of the current batch is determined; in the hydration monitoring window, the chopping rotor torque change and the slurry temperature rise amplitude are continuously collected, and the increase or decline of the slurry viscosity in the protein particle swelling process is identified.

The application discloses a method for preparing salt and heat double-resistant soybean protein powder by using salt-containing preheating combined with micro-jet circulation treatment, and the method comprises the following steps: dispersing soybean protein powder in a phosphate buffer solution, preheating treatment after adding sodium chloride, and then micro-jet treatment; performing modification through circulation "preheating-micro-jet" combined treatment; and finally obtaining salt and heat double-resistant soybean protein powder through freeze drying. In the preheating stage, the method introduces sodium chloride, regulates the electrostatic interaction and hydration between protein molecules, reshapes the protein unfolding path, and promotes the cross-linking and recombination of the protein into controllable intermediate aggregates. Then, the protein is further broken and rearranged through the shearing, impact and cavity effect of high-pressure micro-jet, and the controllable aggregation and dynamic reconstruction are realized through multiple circulation, so that uniform and dense protein particles are finally formed. The soybean protein obtained by the method can still maintain good fluidity after being heated under high-protein concentration and high-salt conditions, and is suitable for salt-containing high-protein beverages and other related fields.

The invention relates to the field of food processing, and discloses a reciprocating single-screw extruder which comprises a base, supporting rings symmetrically and fixedly sleeve the left side and the right side of the upper surface of the base, a mounting shell movably sleeves the middles of the inner curved surfaces of the two supporting rings, and a plurality of pushing mechanisms are arranged on the peripheries of the middles of the two supporting rings at equal intervals. A first sleeve shaft is arranged in the middle of the mounting shell. In the process of extruding the raw materials, the raw materials are ground for many times, so that dietary fibers in the raw materials are broken, and a protein part forms paste with smaller particles, thereby solving the problems that fibers in the dietary fibers in the raw materials play a role of a structural framework in a dry finished product, so that the finished product is too hard and is difficult to bite off; and meanwhile, the problems that the viscosity is insufficient due to overlarge fiber and protein particles in the raw materials, a mixture such as starch needs to be added, a finished product is formed, and the quality of the finished product is reduced are solved.

The application provides a cytoskeleton modeling method based on dissipative particle dynamics simulation, comprising the following steps: step 1, setting a cell region in a dissipative particle dynamics simulation software; step 2, randomly distributing microfilament single chains which do not intersect each other in the cell region; step 3, randomly generating a plurality of free cross-linking protein particles in the cell region; step 4, the microfilament single chains and the cross-linking protein particles are polymerized / dissociated until the number of the cross-linking protein particles in three states tends to be stable, the three states of the cross-linking protein particles are free, combined with only one microfilament single chain and combined with two microfilament single chains; step 5, deleting the cross-linking protein particles and directly connecting two microfilament single chains connected by the same cross-linking protein particle; and step 6, calculating cytoskeleton parameters and outputting a cytoskeleton model. The cytoskeleton constructed by the application has high fidelity and presents mechanical characteristics such as viscoelasticity under the action of an external force.

The invention relates to protein particles comprising a cluster of one or more types of assembled self-contained protein, wherein the particle has a size from 50 nm to 50 micrometers; is in form of a pellet in aqueous media; is mechanically stable; and it release a particular percentage by weight of the self-contained protein within a predetermined period of time, and any other compound contained in the particle. Particular methods for obtaining the particles are also disclosed, said methods comprising the addition of salts to allow precipitation of proteins. Particular protein particles comprising lipids associated with the assembly of self-contained proteins are also disclosed. The invention also relates to several uses of the particle, in particular medical uses and to pharmaceutical and cosmetic compositions comprising the particles.

The present invention relates to viral vectors co-encoding an artificial CD4 T cell epitope (PADRE) for enhanced immune responses to encoded multimeric protein particle displayed vaccine antigens.

The separation method specifically comprises the following steps: (1) centrifuging the supernatant of the liver cancer cell culture medium, enabling the supernatant to pass through a membrane, carrying out ultra-sonar filtration, and then carrying out resuspension to obtain an exosome crude extracting solution; (2) purifying the exosome crude extracting solution by using a size exclusion chromatographic column, and performing ultracentrifugation to obtain exosome crude precipitate; and (3) treating the exosome coarse precipitate by using a membrane protein pulp protein extraction kit, collecting supernate, and carrying out ultracentrifugation to obtain the purified exosome. According to the method, aiming at the characteristics that the exosome membrane structure is relatively large and the shearing force is sensitive, the vesicle aggregate possibly existing in the supernate can be thermally depolymerized through the ultra-sonar filtration, and the lipid bilayer and the biomarker are completely reserved under the mild elution condition of the size exclusion chromatography; and finally, protein particles introduced by fetal calf serum in the complete medium are settled by using a membrane protein plasma protein extraction kit, so that the extracted and separated exosome can directly meet the requirements of NTA particle size verification, proteomics and other experiments.

Particles comprising a protein and optionally an activator, for example, particles comprising whey protein hydrolysate and optionally tryptophan, are described herein. Methods for preparing and using particles comprising a protein and optionally an activator are also described herein.

The invention belongs to the technical field of formula foods for special medical purposes, and particularly relates to a low-potassium low-phosphorus protein component for special medical purposes and a preparation method thereof. The protein component is of a liquid ready-to-use type, and comprises the following components on the basis of 100 mL of total volume: 10.0-12.0 g of separated whey protein powder; and 0.05 to 0.15 g of stevioside. 0.20 to 0.30 g of a compound stabilizer; and the balance of water. The compound stabilizer is prepared from phospholipid and xanthan gum. The separated whey protein powder with low phosphorus and potassium content and specific calcium content is selected as a core protein source, so that the final product has extremely low phosphorus-protein ratio and potassium content. The phosphorus intake brought by the product is obviously lower than that of commercially available like products, and the kidney metabolism burden and the hyperphosphatemia risk of patients are greatly relieved. The phospholipid and the xanthan gum in a specific mass ratio are used as a compound stabilizer, and the phospholipid is used as an emulsifier, so that protein particles can be effectively wrapped, and aggregation is prevented.

The invention discloses a silk fibroin freeze-dried dressing which comprises silk fibroin, mannitol and sodium chloride, a preparation method comprises the steps that raw materials are mixed, stirred and freeze-dried, and the freeze-drying step comprises the steps of pre-freezing, vacuumizing and drying; the crystal network structure of mannitol shortens the redissolution time of the freeze-dried powder, a uniform solution is formed after redissolution, the use convenience is improved, and the use safety risk possibly caused by incompletely dissolved protein particles is reduced; the sodium chloride maintains the ion balance of the protein solution by adjusting the osmotic pressure; and the mannitol is not easy to absorb moisture, so that the long-term stability of the product can be kept in the packaging and storage processes.

The invention discloses a method for constructing rice bran protein-based oleogel based on a capillary bridging effect and application of the oleogel in preparation of a motion energy bar. Rice bran protein dispersed in vegetable oil is combined with polyphenol in a water phase, so that the wettability of rice bran protein particles in an oil phase is regulated and controlled; the rice bran protein particles are induced to form a three-dimensional network through a bridging effect to wrap oil to form oil gel, and liquid vegetable oil is converted into semi-solid gel, so that the gel strength, the oil holding capacity, the storage stability and the freeze-thaw stability are remarkably enhanced, and the steps are simple.

The invention discloses a low-glycemic plant-based food processing method and a low-glycemic plant-based food product. The method comprises the following steps: crushing bulk plant-derived edible agricultural products; the preparation method comprises the following steps: compounding starch, protein, non-starch polysaccharide and functional lipid according to a specific ratio, and adding water for conditioning; and extruding and recombining the mixture in a double-screw extruder at a specific partition temperature and a screw rotating speed to obtain the food. The preferable scheme also comprises the following steps: adding a natural plant extract rich in 3-deoxidized anthocyanin; carrying out enzymolysis fermentation and embedding treatment on the protein to form sustained-release protein particles; a prebiotic sweetening agent composed of xylitol and stevioside and a sugarcane polyphenol plant source composition are added; and carrying out superheated steam treatment on the extruded product. According to the invention, through scientific compounding and extrusion structured recombination cooperation of nutritional components, a compact matrix is constructed, multiple functional components are combined, stable low glycemic content is realized from multiple ways of physical barrier, digestion inhibition, slow release and the like, and the nutrition and palatability of the product are ensured at the same time.