355 results about "Milk protein" patented technology

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-7 and a complex microbial inoculant thereof in sugarcane leaf silage, the strain GX-7 is separated from healthy pig manure by a research group, and through detection, the strain has good cholate resistance and is suitable for surviving in intestinal tracts, and the strain GX-7 has good cholate resistance and is suitable for being used as a feed additive. The antibacterial effect on pseudomonas fluorescens, listeria monocytogenes, salmonella choleraesuis and shigella flexneri is achieved; the strain has a good fermentation capability on sugarcane leaves, and the sugarcane leaves are inoculated with a complex microbial inoculant prepared from streptococcus non-lactolyticus GX-7 and streptococcus non-lactolyticus GX-2, so that a synergistic fermentation effect can be achieved; after the silage prepared from the complex microbial inoculant is fed to female buffalo in the gestation period, it is found that the silage can effectively increase the content of milk protein, milk total solids and milk non-fat solids in colostrum of the female buffalo and relieve diarrhea of the female buffalo, and the silage is safe and good.

The invention discloses a beta-lactoglobulin fluorescent probe as well as a preparation method and application thereof, the structural formula of the probe is (C30H25N4I), the probe is combined with beta-lactoglobulin through non-covalent interaction, a fluorescence signal is triggered to be remarkably enhanced, and low-background fluorescence detection of the beta-lactoglobulin is realized. The probe has low cytotoxicity and excellent mitochondrial targeting ability, and provides a powerful tool for visualization of beta-lactoglobulin in living cells. After being combined with beta-lactoglobulin, a sample can generate obvious color change under the irradiation of an ultraviolet lamp, and the change can be used for evaluating the content of active beta-lactoglobulin in the sample, so that the rapid detection of the milk protein level in a beverage is realized. The beta-lactoglobulin fluorescent probe disclosed by the invention has a wide application prospect in the fields of biological imaging, milk protein detection, beverage quality control and the like.

The invention discloses a method for enriching whey protein main monomers from cow milk. According to the method, whey protein subjected to membrane separation is taken as a raw material, interaction between main monomers of the whey protein and other milk proteins is weakened by adopting a pH conversion technology and an ultrasonic technology, mineral substances, lactose, bovine serum albumin and residual casein are removed through ion exchange chromatography, the main monomers of the whey protein are enriched, and the high-purity whey protein is obtained. And drying the alpha-lactalbumin and the beta-lactoglobulin to obtain a milk protein product rich in two main monomers of the whey protein, and the total content of the two monomers is up to 85% or above. According to the technology, cow milk serves as a raw material, efficient enrichment of two main monomers of whey protein can be achieved only through membrane separation, pH conversion, ultrasonic assistance and ion exchange chromatography, only pH needs to be adjusted in the separation process, other chemical reagents are not added, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in the whey protein main monomer can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

This invention discloses a milk protein containing composition comprising: at least one milk protein; at least a first protein; at least a second protein; and at least a linker comprising an amino acid sequence coded by nucleotide sequence which is at least 80% similar to sequences selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention provides cream cheese and a preparation method thereof, and belongs to the technical field of dairy product processing. According to the method, firstly, raw milk is subjected to degreasing and membrane filtration concentration, the concentration multiple is controlled to be 1.5-4, the lactose content of the concentrated solution is precisely regulated and controlled to be 3-6.5% through washing filtration, and the raw milk concentrated solution rich in casein (accounting for 85-95% of total protein) is obtained. Then, mixing the concentrated solution with single cream, white granulated sugar and the like, homogenizing, sterilizing, inoculating a leavening agent and chymosin, fermenting, refrigerating and demulsifying to obtain a finished product. According to the invention, nutrition waste and environmental pollution caused by discharge of whey in a traditional process are fundamentally avoided through membrane filtration pretreatment, and meanwhile, the problems of pink feeling and peculiar smell caused by addition of milk protein powder are solved; the obtained product is pure and rich in flavor, fine and smooth in texture, simple and efficient in process and suitable for large-scale production.

The utility model relates to the technical field of drying equipment, and discloses milk protein fiber blended fabric drying equipment. The milk protein fiber blended fabric drying equipment comprises a main body and an extension column located in the main body; the inner wall of the main body is connected with one side of a fixing ring through a fastener, the side, away from the main body, of the fixing ring is connected with a rotating roller in a rotating mode, the top of the fixing ring is connected with the bottom of a supporting block in a welding mode, an extension column moves to drive a tightening roller to move, and the tightening roller drives fabric on the tightening roller to stretch; the length of the extension column can be shortened so that the extension column can expose a large area in the main body, the extension column can slowly pass through the interior of the main body, when the fabric is dried quickly, the length of the extension column can be shortened so that the fabric can quickly pass through the interior of the main body, the drying equipment can be adjusted according to different fabrics, a long conveying device is not needed, and the drying efficiency is improved. And the occupied area of the drying device is effectively reduced, and the practicability of the device is embodied.

The invention discloses a preparation method and application of a cow milk antibacterial peptide with wide inhibitory activity on gram-positive bacteria and gram-negative bacteria by a fermentation method, the cow milk antibacterial peptide is prepared by taking milk protein as a raw material through a microbial fermentation method and a multi-stage membrane separation and purification process, the yield of the cow milk antibacterial peptide prepared by the method can reach 18-30%, and the yield of the cow milk antibacterial peptide prepared by the method can reach 18-30%. And the peptide content in the product is 60-85%. The cow milk antibacterial peptide prepared by the preparation method disclosed by the invention has inhibitory activity on gram-positive bacteria and gram-negative bacteria. The prepared cow milk antibacterial peptide can be used for preparing drugs and / or health care products, food and skin care products for preventing and / or reducing infectious diseases caused by escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, porphyromonas gingivalis and streptococcus mutans, can also be used as a food preservative, an oral bacteriostatic agent, a preservative and the like, and has a good application prospect.

The invention relates to the technical field of cream cheese preparation, in particular to whey-free natural fruity original cream cheese and a preparation method thereof.The whey-free natural fruity original cream cheese comprises a raw material bag, and the raw material bag comprises, by mass, 45-60 parts of single cream, 20-30 parts of concentrated milk, 20-30 parts of milk protein powder, 20-30 parts of table salt, 20-30 parts of a stabilizer and 20-30 parts of a composite leavening agent; the mass part of the concentrated milk is 35-50; 4-7 parts by mass of milk protein powder; the mass part of the table salt is 0.5-1.5 parts. The cream cheese with natural fruity flavor is prepared, high-index single cream and concentrated milk protein are used as raw materials, through a specific fermentation technology, pleasant fruity flavor can be generated without additional addition of flavor substances, and the flavor category of original cheese is enriched. And a semi-finished product can be obtained without discharging whey after fermentation, so that the process is simplified, the efficiency is improved, the problems of microbial risk and texture deterioration caused by addition of jam and secondary heat treatment in the traditional processed cheese are avoided, and the safety and quality of the product are remarkably improved.

The invention discloses a bone-strengthening lactoprotein extract as well as a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains bone-strengthening active peptide FYPELFR. The preparation method specifically comprises the following steps: (1) pasteurization; (2) separating casein; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purifying and desalting; and (6) concentrating and drying. The bone-strengthening active peptide and the bone-strengthening lactoprotein extract containing the bone-strengthening active peptide can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonds, proliferation and differentiation of osteoblasts are promoted by activating a specific signal channel, proliferation of the osteoblasts is promoted, and the bone-strengthening active peptide and the bone-strengthening lactoprotein extract can be used for development of bone-strengthening functional food or drugs and have important significance on deep processing of cow milk.

The invention discloses a preparation method of gelatinized euphausia superba oil microcapsules, which comprises the following steps: selecting glycerol laurate as an emulsifier, mixing and stirring the glycerol laurate and euphausia superba oil, standing to obtain oleogel, mixing the oleogel as a core material with a wall material consisting of a mixture of concentrated milk protein, whey powder and corn syrup to prepare an emulsion, and preparing the gelatinized euphausia superba oil microcapsules. And preparing the gelatinized euphausia superba oil microcapsule by adopting a spray drying method. The gelatinized microcapsule prepared by the method disclosed by the invention is relatively uniform in distribution, relatively smooth in capsule body surface and relatively complete in capsule wall, and the euphausia superba oil can be well embedded by a wall material to present a one-capsule multi-core structure. The gelated euphausia superba oil microcapsule has good compactness, can effectively mask the bad smell of the euphausia superba oil, and improves the sensory performance of the euphausia superba oil.

The invention discloses a soft shell jacket composite fabric and a preparation method thereof, and aims at solving the problem that an existing soft shell jacket fabric is poor in waterproof performance. According to the technical scheme, the fabric is characterized by comprising a waterproof layer, a warm-keeping layer and a skin-friendly layer which are sequentially arranged from top to bottom, and the skin-friendly layer is prepared from, by weight, 40-60 parts of cotton fibers, 10-15 parts of milk protein fibers, 10-25 parts of bamboo pulp fibers and 20-45 parts of antibacterial fibers; the surface of the waterproof layer is coated with a waterproof finishing agent, and a waterproof film layer is fixedly bonded to the surface of the waterproof layer. The waterproof performance of the fabric is enhanced by arranging the waterproof layer, the warm-keeping effect and comfort of the fabric can be considered by arranging the warm-keeping layer and the skin-friendly layer, the fabric is more comfortable to wear, and outdoor requirements under more conditions are met.

Provided herein are compositions and methods for producing milk proteins, which allow for safe, sustainable and humane production of milk proteins for commercial use, such as use in food compositions. The disclosure provides recombinant fusion proteins comprising at least first protein and a second protein, wherein at least one of the first protein and the second protein is a milk protein, or fragment thereof. The disclosure also provides methods for producing the recombinant fusions proteins, and food compositions comprising the same.

The invention relates to the technical field of non-dairy creamer, in particular to acid-resistant non-dairy creamer and a preparation method thereof. The acid-resistant non-dairy creamer provided by the invention is prepared from the following components in percentage by mass: 20 to 35 percent of vegetable oil, 5 to 18 percent of modified starch, 3 to 12 percent of Arabic gum, 0.3 to 1.5 percent of fatty acid monoglyceride, 0.2 to 1 percent of sodium stearoyl lactylate, 0.2 to 0.5 percent of silicon dioxide and the balance of glucose syrup. The acid-resistant modified starch, the Arabic gum and other emulsion stabilizers are added to replace milk protein, the glucose syrup with better film-forming property is used as the wall material, and the obtained non-dairy creamer is good in acid resistance, so that the problem that the non-dairy creamer product is not acid-resistant in the prior art is solved. The result of the embodiment shows that the acid-resistant non-dairy creamer provided by the invention is completely stable under the acidic condition that the pH value is 3.5-6, and the phenomena of layering, floating or precipitating do not occur.

The invention discloses an SNP molecular marker for increasing the milk yield of Holstein cattle and application of the SNP molecular marker, the SNP molecular marker is located at a mutation site of a Holstein cattle genome Leptin gene, the gene number of the Leptin gene in an NCBI database is NC037333.1, the SNP molecular marker is located at the 1761th basic group of a cDNA sequence of the Leptin gene, and the mutation basic group is A or G. When the basic group of the SNP molecular marker is A, the genotype is AA or AG, and the milk fat rate and the milk protein rate of Holstein cow milk production are high; when the basic group of the SNP molecular marker is G, the genotype is GG, the milk yield of Holstein cattle in 305 days is high, and the milk quality is not obviously reduced. The marker is applied to genetic improvement of dairy cows, and the frequency of dominant alleles can be improved generation by generation by selecting individuals carrying the dominant alleles, so that the lactation performance of the dairy cows is effectively improved, the breeding efficiency is optimized, and the competitive advantage in the field of dairy cow breeding is enhanced.

The invention discloses a nanopore filtering and separating medium as well as a preparation method and application thereof, a polymer-based nanopore medium with accurate pore size distribution is constructed through a template-induced self-assembly technology, and efficient interception of milk fat components and selective permeation of whey protein are realized in combination with a surface functionalization modification strategy. Based on a size screening and electrostatic repulsion synergistic mechanism, milk fat enrichment and milk protein purification are synchronously completed under mild operation conditions, and energy consumption and component loss in a traditional process are remarkably reduced. And the long-term operation stability is maintained by regulating and controlling the structure parameters and surface chemical characteristics of the nano-pore channel. The technology provides an innovative solution for developing high-purity buffalo milk fat products and defatted buffalo milk products.

The invention provides a milk protein peptide which takes milk protein as a raw material and has the effects of promoting calcium absorption and improving bone health and application of the milk protein peptide. Specifically, milk protein peptide sequences, including YGGVSL and SQRY, are screened by adopting enzymolysis, sequence identification and molecular docking methods. The milk protein peptide is proved to have high calcium transport promoting capacity in the in-vitro intervention of Caco-2 cells, and the milk protein peptide is proved to have the capacity of promoting osteogenesis and inhibiting bone resorption in the in-vitro intervention of MC3T3-E1 mouse embryo osteoblasts. In animal experiments, by increasing the content of blood calcium and bone calcium, reducing the content of excrement calcium, improving mRNA expression of calcium ion channel protein, calcium binding protein and peptide transporter in intestinal tracts, enhancing the efficiency of calcium entering blood, promoting osteogenesis and inhibiting bone resorption, the milk protein peptide is comprehensively proved to have the capabilities of improving calcium resorption of organisms and improving bone health. The milk protein peptide has both functionality and nutrition, and has a wide application prospect in the aspect of bone health.

The invention discloses an anti-muscle attenuation composition and application thereof, and the composition provided by the invention comprises an anti-muscle attenuation peptide, WPC, MLCT and CaHMB; the muscle attenuation resisting peptide is zymolyte obtained after milk protein is subjected to protease enzymolysis, the zymolyte comprises polypeptide with the absolute net charge density ridge-shaped region distribution of a peptide chain formed by a head peptide fragment, a middle peptide fragment and a tail peptide fragment, the head peptide fragment is j1, the middle peptide fragment is j2, and the tail peptide fragment is j3; the charge density of the j1 and the charge density of the j3 are both larger than the charge density of the j2, or the charge density of the j1 and the charge density of the j3 are both smaller than the charge density of the j2. Through mutual cooperation of the muscle attenuation resisting peptide, the WPC, the MLCT and the CaHMB, the effect of obviously improving the muscle attenuation resisting effect is achieved.

The invention discloses a culture medium for A type clostridium perfringens and C type clostridium perfringens, a fermentation culture method and application of fermentation toxin production. Wherein the culture medium comprises the following components: tryptone, # imgabs0 # peptone, casein peptone, a yeast extract, lactoalbumin hydrolysate, trihydroxymethyl aminomethane, sodium acetate and glucose. According to the present invention, through the specific formula composition, the A-type clostridium perfringens and the C-type clostridium perfringens can adapt to fermentation in the fermentation tank, and in the fermentation culture process, the toxin production ability of the A-type clostridium perfringens and the C-type clostridium perfringens can be significantly improved by controlling the stirring speed, the pH value of the fermentation liquid and the precise material supplementing manner. And the supernate obtained by fermentation is used as a raw material for preparing the vaccine, so that animals can generate high-level neutralizing antibodies aiming at the clostridium perfringens A and C type toxins after being immunized with the vaccine, the death of the animals caused by infection of the clostridium perfringens A and C type toxins is avoided, and the method has practical popularization and application values.

The invention discloses camel milk protein-polysaccharide composite nanoparticles and a preparation method thereof, and belongs to the technical field of preparation of natural fermentation composite particles. The preparation method comprises the following steps: (1) inoculating phytobacterium plantarum into camel milk for fermentation, centrifuging, and collecting supernatant; and (2) carrying out ultrasonic treatment on the supernatant under an ice-water bath condition, adding absolute ethyl alcohol to precipitate after the ultrasonic treatment is finished, standing, centrifuging, and dialyzing to remove impurities, so as to finally obtain the camel milk protein-polysaccharide composite nanoparticles. According to the preparation method, camel milk is taken as a fermentation substrate, fermentation is carried out by utilizing phytobacterium plantarum LR-1, and the natural polysaccharide-protein nanoparticles (PPNPs) are successfully prepared in combination with ultrasonic treatment. In the fermentation process, the metabolic activity of lactic acid bacteria promotes the combination of protein and polysaccharide, and the particle size, the stability and the oxidation resistance are improved; the ultrasonic treatment further optimizes the particle size and structure, and enhances the oil-water interface adsorption capacity.

The invention provides an anti-osteoporosis milk-derived peptide YPSYG and application thereof, and a preparation method of the anti-osteoporosis milk-derived peptide YPSYG comprises the following steps: taking milk protein, firstly adding papain for enzymolysis, and then adding alkaline protease for enzymolysis to obtain the milk-derived peptide YPSYG. The milk-derived peptide with bone synthesis promoting activity can significantly improve bone biomechanical characteristics and bone microstructure, and can be used for preparing anti-osteoporosis drugs for treating osteoporosis. The milk-derived peptide YPSYG disclosed by the invention is convenient to synthesize, can be industrially produced, and has a good application prospect in the fields of medicines, cosmetics, health-care products and the like.

The invention discloses a milk protein base material rich in beta-casein and a preparation method of the milk protein base material. The method comprises the following steps: firstly, obtaining a casein micelle trapped fluid through a membrane separation technology, and secondly, promoting casein micelles to be partially dissociated and partially precipitated by adopting a pH conversion combined ultrasonic technology, so that the interaction between a casein monomer and whey protein is destroyed; adjusting the pH value of the solution to be neutral, and dissolving the casein; the preparation method comprises the following steps: adding calcium chloride to promote the aggregation of the alpha-casein by utilizing the sensitivity of the alpha-casein to calcium ions, and intercepting the alpha-casein by utilizing a membrane separation technology to realize the enrichment of the beta-casein so as to prepare the milk protein base material rich in the beta-casein. Compared with the traditional column chromatography technology, the technology has the advantages that the efficient enrichment of beta-casein and the efficient separation of alpha-casein can be realized through the membrane separation and pH conversion combined ultrasonic technology; in the separation process, only pH needs to be adjusted, other auxiliaries are not needed, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in beta-casein can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The invention discloses a sea cucumber aqueous extract anti-helicobacter pylori solid beverage and a preparation method thereof. The solid beverage comprises the following substance components in parts by weight: 10-15 parts of a sea cucumber-hericium erinaceus aqueous extract mixture, 20-30 parts of resistant dextrin, 0.5-1 part of whey protein powder and 2-4 parts of xylitol. The preparation method comprises the following steps: obtaining the sea cucumber extract in a water extraction and alcohol precipitation manner; the hericium erinaceus extract is obtained in a water extraction and alcohol precipitation mode; the sea cucumber extract and the hericium erinaceus extract are redissolved, homogenized and then spray-dried, resistant dextrin, whey protein and xylitol are added, the mixture is mixed evenly and directly bagged, and the sea cucumber aqueous extract anti-helicobacter pylori solid beverage is obtained. The solid beverage disclosed by the invention has direct helicobacter pylori resisting activity in vitro, solves the problems of increased bacterial drug resistance, low eradication rate and large side effect in the existing quadruple therapy for treating helicobacter pylori, and can promote the deep processing of sea cucumbers and improve the additional value of sea cucumbers.

A powder contains a buffer salt and an amino acid, for example one or both of (i) least one glycine or functional derivative thereof or (ii) at least one N-acetylcysteine, L-cysteine or functional derivative thereof. Optionally the powder additionally includes at least one vitamin or mineral. Preferably the powder has at least one characteristic selected from the group consisting of (i) the amino acid is present in an amount that at least partially comprises the amino acid in a form selected from the group consisting of free form, dipeptides, tripeptides and mixtures thereof and (ii) the powder is substantially free of enzymes and antibodies. The powder can be reconstituted in a liquid containing an acidic component, such as juice or a polyphenol, and / or a protein, such as milk protein, plant protein, collagen or blends thereof. The buffer salt can minimize or prevent denaturation of protein and / or coagulation of protein upon reconstitution of the powder in the liquid.

The invention relates to the field of food engineering, in particular to a fruit grain sandwich yoghourt cheese bar and a preparation method thereof. The fruit granule sandwich yogurt cheese stick comprises a core material and a skin material, the core material comprises fresh fruit granules and / or jam accounting for 25-35% of the weight of the core material, and the core material and the skin material both contain natural cream cheese, concentrated milk protein and yogurt, so that higher nutritional value is provided for the cheese stick. The fruit grain sandwich yoghourt cheese rod provided by the invention not only has various tastes and rich nutrition, but also can obtain the yoghourt cheese rod which is stable in structure and contains granular fresh fruit grains in the core material under the condition of not using more additives, and brings good sensory experience to consumers.

The invention relates to the technical field of gene engineering, in particular to a method for reducing acetic acid accumulation in the process of ethanol-induced expression of alpha-lactalbumin by regulating pichia pastoris. According to the recombinant yeast strain provided by the invention, the accumulation amount of acetic acid is greatly reduced in the process of inducing expression of target protein by adopting ethanol, acetic acid can be basically not accumulated, relatively high biomass and target protein yield can be achieved, and the highest yield of human alpha-lactalbumin up to now is achieved; and a strain resource and a foundation are provided for application of an ethanol-induced pichia pastoris expression system and development of bioengineering milk protein. The invention provides a method for solving the problem of acetic acid accumulation of pichia pastoris in the process of inducing expression of the target protein by using ethanol, and the method provides an effective strategy for popularization and application of an ethanol-induced expression system and improvement of the yield of the target protein induced and expressed by using the ethanol. The method has important significance on production of proteins such as alpha-lactalbumin and the like.

To provide a paste pie dough capable of stably obtaining a paste pie product having a crispy and light texture and less shrinkage due to baking, and particularly, enabling molding by a bean jam wrapping machine.SOLUTION: A paste pie additive agent contains milk-derived phospholipid and milk protein, and in which the additive agent is an aqueous solution containing the milk protein of 1-15 pts.mass relative to 1 pt.mass of the milk-derived phospholipid.SELECTED DRAWING: None

The present invention discloses a targeted knockout bovine FGFR1 The application of the sgRNA of the gene in constructing a bovine mammary epithelial cell line, wherein the sgRNA nucleotide sequence is shown in SEQ ID NO: 1. The mammary epithelial cell line obtained by constructing the sgRNA of the present invention through CRISPR / Cas9 technology, FGFR1 Protein expression was significantly downregulated, and knockout FGFR1 The gene expression had no significant effect on the viability and proliferation of mammary epithelial cells. PCNA 、 Cyclin D1 、 Cyclin E1 There was no significant change in the mRNA expression of the genes related to milk protein synthesis (such as CSN1S1 、 CSN2 、 CSN3 、 EFL5 ), lactose synthesis-related genes ( GLUT1 and HKⅡ ) and milk fat synthesis-related genes ( SCD‑1 ) were significantly decreased, providing a basis for in-depth analysis FGFR1 It provides key tools for the regulatory mechanisms of mammary epithelial cell proliferation, lactation synthesis and other biological behaviors.

The present disclosure addresses the problem of providing a bakery food product mix that contains 50 mass% or more of whole wheat flour and can produce bakery food products having good baked color, soft texture, and rising properties.　The bakery food product mix contains 50 mass% or more of whole wheat flour, milk protein, and soybean protein.

The invention provides application of a rice endosperm protein content gene, and belongs to the technical field of biology. According to the application, the OsAFB3 gene is used for regulating and controlling the content of the rice endosperm protein. According to the application of the rice endosperm protein content gene provided by the embodiment of the invention, the OsAFB3 gene is used for regulating and controlling the content of the rice endosperm protein, and the effect is remarkable.