6results about How to "Avoid immune response" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant human III-type collagen for promoting angiogenesis as well as a preparation method and application of the recombinant human III-type collagen. The recombinant human III-type collagen for promoting angiogenesis has an amino acid sequence as shown in SEQ ID NO. 1. Nine different sites of the full-length natural III-type collagen are replaced by GFOGER sequences which are from the I-type collagen and are specifically combined with integrin sites, the angiogenesis promoting function of the collagen is enhanced under the condition that the total length of natural full-length amino acid is not changed, and meanwhile, four natural integrin sites with low adhesion rate are also replaced; the recombinant III-type collagen has high adhesive force, so that angiogenesis is further promoted. By means of the transformation mode, the angiogenesis rate is increased by 37% or above, the molecular weight of the translated protein is moderate, and the translated protein is easy to prepare.

The invention discloses a flexible electrode and a preparation method and application thereof, and relates to the technical field of medical instruments. The flexible electrode comprises a flexible substrate layer, a conductive electrode layer, a packaging layer and an electrode contact which are arranged in a stacked mode. The conductive electrode layer comprises a wire and a conductive contact which are communicated with each other; the packaging layer is provided with a through hole structure, and the through hole structure exposes the conductive contact; and the electrode contact is arranged on the conductive contact. The electrophysiology mapping and electroporation functions are integrated, switching and operation complexity among multiple devices is reduced, and organic combination of real-time monitoring and accurate treatment is achieved.

The application relates to the technical field of bioengineering, and particularly discloses a recombinant human collagen XV and a preparation method and application thereof. The amino acid sequence of the recombinant human collagen XV is shown as SEQ ID NO. 1. The recombinant human collagen XV is derived from human collagen, and has no rejection and sensitization risks. Experiments show that the protein has good effects of promoting cell proliferation and promoting cell migration, and has good biocompatibility, and can be applied as a safe and effective biomedical material.

The application discloses a modular dual translation regulation molecule system and a construction method and application thereof, relates to the fields of synthetic biology and nucleic acid engineering. The translation regulation system is an artificially optimized single-stranded mRNA molecule, which orthogonally integrates a translation inhibition module (OFF switch) and a translation activation module (ON switch) in the same transcript. The mRNA molecule sequentially integrates the following elements: a 5' end cap structure, a 5' untranslated region, an open reading frame of a target protein, a single-copy inhibitory miRNA binding site inserted at a specific position upstream of the 3' untranslated region, a poly-A tail, an activating miRNA binding site (responsive to miR-21), and a translation inhibition element. The application realizes precise regulation by using the difference in endogenous miRNA expression abundance between target cells and non-target cells, and is safer in use.

The utility model discloses a bird repelling device which comprises a bottom plate installed on a cross arm of a telegraph pole, a connecting plate is installed on the bottom plate, long spine layers and short spine layers are arranged on the connecting plate in the length direction of the connecting plate at intervals, and each short spine layer is located between every two adjacent long spine layers. The long spine layer is composed of a plurality of long spines which are evenly arranged at intervals in the direction perpendicular to the length direction of the connecting plate. The short spine layer is composed of a plurality of short spines which are evenly arranged at intervals in the direction perpendicular to the length direction of the connecting plate. The top of the bird repelling device is of the conical spine structure which simulates wild jujube spines, so that when birds fall on the bird repelling device on a cross arm of a telegraph pole, the birds feel pain caused by the spines and actively fly away from the area, and the conical tips of the tops of the spines are of the smooth structure and do not cause great harm to the birds. Birds are not damaged while efficient bird repelling is achieved, and the situation that the birds generate immune reaction and continue to nest on the telegraph pole cross arm is avoided.

The application provides a poly(2-ethyl-2-oxazoline) block copolymer containing a sulfobetaine side group and a preparation method and application thereof. The copolymer prepared by the application has enhanced biocompatibility and stronger water absorption capacity due to the introduction of the sulfobetaine side group (the water absorption capacity of which is the strongest among zwitterionic groups) and poly(2-ethyl-2-oxazoline), and is more conducive to forming a hydration layer in a living body, so that water molecules repel the approach of immunoglobulins, thereby avoiding causing an immune response, and is expected to have a potential and huge development prospect in the field of biomedical materials. The copolymer of the application can be applied to drug loading and has excellent drug loading capacity and a slow release effect.