214 results about "Type III collagen" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The invention relates to the technical field of biology, and particularly discloses a recombinant collagen III and application thereof in preparation of gel. The recombinant collagen III is designed by optimizing functional area sequences of human I-type and III-type collagen, the amino acid sequence is shown as SEQ ID No.2, and the recombinant collagen III has the characteristics of high stability, good hydrophilicity and low immunogenicity. The preparation method comprises the steps of expression vector construction, escherichia coli induced expression, affinity chromatography purification and renaturation. The recombinant collagen III can be prepared into a gel dressing and comprises sodium alginate, methylparaben and other components. Experiments show that the gel can effectively promote cell proliferation and has no cytotoxicity; in a mouse skin injury model, the collagen can relieve inflammation, accelerate wound healing and inhibit scar formation, and the effect of the collagen is superior to that of natural human III-type collagen. The invention provides a safe and efficient novel material for wound repair, and is suitable for medical dressings and tissue engineering.

The invention discloses humanized III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. According to the method, firstly, on the basis of an original humanized III-type collagen gene, cell adhesion activity is taken as an evaluation index, a better cell adhesion sequence is screened out and spliced and integrated with the original collagen gene, and brand-new humanized III-type collagen is obtained. And constructing a recombinant expression vector of the humanized III type collagen containing the adhesion sequence by taking the probiotic EcN as a host cell, and introducing the recombinant expression vector into the EcN to obtain a strain of functional recombinant probiotic. Compared with a traditional escherichia coli engineering bacterium BL21 (DE3), the recombinant probiotics have good cell adhesion activity while efficiently expressing the humanized type III collagen, the bacterial endotoxin content of the recombinant humanized type III collagen obtained through purification meets the industrial standard, and the recombinant probiotics have good application prospects. The purification cost caused by industrial removal of endotoxin can be avoided, so that the production cost is reduced, and the method has a good application prospect.

The invention relates to the technical field of biological medicine, in particular to recombinant III-type humanized collagen as well as a preparation method and application thereof. In one embodiment, the recombinant III type humanized collagen prepared by the invention has an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 4. The recombinant III-type humanized collagen prepared by the invention has good biological activity, can promote collagen synthesis related gene expression, promote skin fibroblast proliferation, increase the content of skin collagenous fibers and elastic fibers, and improve skin elasticity and compactness; in addition, the expression of osteogenesis related genes is promoted, and the proliferation of osteoblasts is promoted. The results show that the recombinant III-type humanized collagen prepared by the invention has an extremely wide application prospect in developing related medical and cosmetic products for delaying skin aging and promoting wound repair and bone tissue healing.

The invention relates to a fibrous membrane, a keratinized gingival repair membrane containing the fibrous membrane and a preparation method of the keratinized gingival repair membrane. The fiber membrane is a recombinant collagen-polycaprolactone fiber membrane containing zinc oxide nanoparticles, and is prepared by the following steps: dissolving recombinant III-type collagen and polycaprolactone in hexafluoroisopropanol, adding the zinc oxide nanoparticles, and uniformly stirring to form a spinning solution; and then carrying out electrostatic spinning. The fiber membrane is good in strength, hydrophilicity, softness, antibacterial performance and the like, when the fiber membrane is applied to the repairing membrane, compared with an existing repairing membrane, the mechanical property, the antibacterial performance and the biological activity are remarkably improved through the bionic three-layer structural design, and a more effective solution is provided for keratogingival repairing.

The invention provides a fusion protein for promoting proliferation of hair follicle stem cells as well as a preparation method and application of the fusion protein. The fusion protein is formed by fusion expression of a plurality of collagen structural domains capable of being combined with cell integrin and / or specific structural domains from certain functional proteins screened from collagen XVII and III type collagen. Nucleic acid molecules for coding the fusion protein are subjected to codon optimization and then recombined and stably expressed in escherichia coli to obtain the fusion protein. The fusion protein has excellent performance of promoting proliferation and migration of human hair follicle stem cells and human fibroblasts, can be used for repairing skin and hair follicle tissues and improving resistance of skin and hair follicles, and is suitable for popularization and application in the fields of beauty and skin care, medical equipment, tissue engineering and the like.

The present application provides a recombinant human type III collagen with high thermal stability and high transdermal permeability. The recombinant type III collagen comprises a type III collagen part and a transdermal peptide part, which has a high transdermal absorption capacity and has the advantage of high thermal stability, and is suitable for the preparation of cosmetics, drugs and medical devices.

The present invention relates to the technical field of protein engineering and provides a recombinant humanized type III collagen having a triple helix structure, a preparation method therefor, and a use thereof. The amino acid sequence of the recombinant humanized type III collagen comprises n core units, wherein n is 1 or an integer greater than 1, and the amino acid sequence of the core unit is as shown in SEQ ID NO: 1. The present invention further provides a method for constructing an expression system and a method for preparing a recombinant humanized type III collagen. The present invention further provides an actual industrial use on the basis of the characteristics of the protein. The recombinant humanized type III collagen provided by the present invention has high purity, good stability, and high biological activity. Experiments have shown that the recombinant humanized type III collagen has various biological activities such as promoting cell proliferation and migration.

The invention discloses Dong'e donkey-hide gelatin active peptide and application thereof. Relates to the technical field of biology. The invention provides an amino acid sequence and application of the Dong'e donkey-hide gelatin active peptide. The amino acid sequence of the CCA active peptide is YYTSASGDEMVSLK, and the CCA active peptide can significantly up-regulate the I-type collagen gene expression, increase the I-type and III-type collagen content and reduce senescence markers. The peptide fragment can be combined with TGFBR2, especially TGFBR1, gene and protein expression of the TGFBR2 can be up-regulated, downstream targets of a TGF-beta-SMAD pathway can be activated, and the MMPs / TIMPs ratio can be reduced. According to the present invention, the TGF beta-SMAD pathway is activated, the MMPs / TIMPs ratio is reduced, the collagen synthesis is promoted, and the excessive decomposition of the collagen is inhibited, such that the I type collagen content and the III type collagen content are increased, and the aging degree of the photoaging dermal fibroblast is reduced;

The invention discloses an anti-infection ointment for promoting healing of burns and scalds and other wound surfaces and a preparation method thereof, the anti-infection ointment comprises active components and auxiliary materials, the active components comprise 0.2-3 parts by weight of ganoderma lucidum chitosan, 0.2-5 parts by weight of recombinant III type collagen, 0.1-2 parts by weight of zinc and 0.5-8 parts by weight of a Cur-Mg compound; the Cur-Mg compound is a compound of Cur-Mg (at) PP hydrogel, antibacterial polyphenol and boric acid ester bond hydrogel. Compared with the prior art, the ointment disclosed by the invention has the advantages of good antibacterial property, high wound healing promoting speed, reduction of scar formation and the like through a synergistic effect of multiple components, and realizes slow release of active components and good wound microenvironment regulation through a hydrogel system.

The application discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application thereof, and belongs to the technical field of cosmetics and skin care products. The composition contains the following active ingredients in percentage by mass: 0.01%-20% of recombinant humanized type I collagen, 0.01%-20% of recombinant humanized type III collagen, 0.01%-10% of recombinant humanized type XVII collagen, and 0.01%-20% of space-bred desert algae extract. The three kinds of recombinant humanized collagens and the space-bred desert algae extract form a complex system, and when the total mass ratio is in a specific interval of 1.3:1-4:1, the three kinds of recombinant humanized collagens and the space-bred desert algae extract can synergistically improve the skin barrier function. Based on the synergistic mechanism of the complex system, the composition disclosed by the application can effectively promote the skin moisture retention and anti-wrinkle ability, repair the skin barrier, and is suitable for the development and application of skin care products for anti-wrinkle, skin tightening and skin aging delay.

The invention provides a recombinant human collagen III with high thermal stability and high transdermal property, and the recombinant collagen III comprises a collagen III part and a transdermal peptide part, has high transdermal absorption capacity, has the advantage of high thermal stability, and is suitable for preparing cosmetics, medicines and medical instruments.

The invention relates to application of recombinant humanized III-type collagen in preparation of an esophageal squamous cell carcinoma inhibitor. The invention discovers that the recombinant humanized III-type collagen can significantly inhibit the proliferation of esophageal squamous carcinoma cells and prevent the generation of malignant phenotypes of esophageal squamous carcinoma cells. Therefore, the invention can be used for preparing medicines for preventing and treating esophageal squamous carcinoma.

The invention discloses a freeze-dried essence with a repairing effect and a preparation method, and belongs to the field of skin care products, and the freeze-dried essence is composed of freeze-dried powder and an independent solvent liquid. The freeze-dried powder contains 0.5-3 parts of palmitoyl oligopeptide, 3-8 parts of I-type collagen, 1-5 parts of III-type collagen, 0.1-1 part of chitosan, 1-3 parts of secondary variable-temperature fermentation liquor of betula platyphylla and 2-8 parts of a freeze-drying protective agent; the solvent liquid is prepared from 0.5 to 3 parts of hyaluronic acid and 0.05 to 0.5 part of sodium polyglutamate. The preparation method comprises the following steps: a, adding fermentation liquor after dissolving; b, adding nano liposome and a protective agent; c, quickly freezing to-55 + / -2 DEG C by liquid nitrogen, and preserving heat for 2.5-3.5 hours; d, drying for 9-11 hours under the vacuum of 15-25 Pa and at the temperature of-18 + / -2 DEG C; and e, the temperature is increased to 15 + / -2 DEG C in a stepped mode, vacuum is smaller than or equal to 20 Pa, and drying is conducted for 18- Through scientific formula design, multiple effects of efficient repairing, anti-aging and relieving are achieved.

The invention provides preparation and application of an improved recombinant III-type collagen peptide. The collagen comprises an amino acid sequence as shown in SEQ ID NO.1, and a tag sequence is fused at the N terminal and / or C terminal of the amino acid sequence as shown in SEQ ID NO.1. The recombinant collagen provided by the invention only contains a small affinity tag during expression, is convenient to purify and does not need enzyme digestion, so that the process steps are greatly simplified, the process cost is reduced, the excellent characteristics of the original recombinant collagen peptide Co8 (SEQ ID NO.1) are maintained, and the recombinant collagen still has high expression quantity, high hydrophilicity and high stability.

The invention discloses a recombinant humanized type-A type-III collagen, a preparation method thereof and a recombinant bacterium, and relates to the technical field of bioengineering. According to the preparation method, peptide fragments with 100% identical sequences of III-type collagen from human are integrated into host bacteria to obtain recombinant bacteria for efficiently synthesizing recombinant humanized A-type III-type collagen, and the recombinant humanized collagen produced by utilizing the recombinant bacteria does not contain any gene sequence of non-human collagen sources, and has the advantages of high efficiency, high yield, high yield and the like. The collagen has the characteristics of small molecular weight, high yield, high purity, water solubility and the like, and can be widely applied to the fields of skin care products, medical cosmetology, skin repair dressings and the like, so that the recombinant humanized A type III collagen, the preparation method thereof and the recombinant bacteria provided by the invention have better industrial application prospects.

The invention provides a medical-grade recombinant three-type collagen and application thereof. The recombinant humanized three-type collagen has certain cell proliferation promoting capacity and is free of cytotoxicity. The medical-grade recombinant three-type collagen provided by the invention not only has the capability of promoting cell proliferation, is free of cytotoxicity and high in cell adhesion activity, but also has a three-helix structure characterized by the collagen and is extremely high in stability, the purity can reach about 85% after the collagen is induced for 120H in a 100L fermentation tank, the difficulty of a downstream purification process is greatly reduced, and the production cost is reduced. The effect in the aspect of cell activity is obvious.

The invention specifically discloses recombinant humanized III-type collagen as well as a preparation method and application thereof, and relates to the technical field of collagen sequence optimization. The invention provides a type III collagen, wherein the type III collagen has an amino acid sequence as shown in SEQ ID No.2. The invention also provides a preparation method of the type III collagen. The III-type collagen provided by the invention not only has no obvious cytotoxicity to cells, but also has an extremely good cell proliferation promoting effect, can also improve cell adhesion, and has an obvious promoting effect on proliferation of human immortalized epidermal cell lines, namely wound healing.

The embodiment of the invention discloses a recombinant III-type humanized collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant III-type humanized collagen is shown as SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3. The recombinant III-type humanized collagen has the advantages that the recombinant III-type humanized collagen can be used for preparing the recombinant III-type humanized collagen; the recombinant III-type humanized collagen provided by the invention has the activity of promoting cell adhesion, the amino acid sequence of the recombinant III-type humanized collagen is selected from the amino acid sequence of natural collagen, and the recombinant III-type humanized collagen does not generate immune response when being applied to a human body. The characteristic that corynebacterium glutamicum does not secrete endotoxin is utilized, the preparation method is simple, the product can meet the requirements of food, cosmetics, medical instruments and drugs without complex separation, and reference is provided for low-cost industrial production.

The invention discloses an enzymolysis preparation process of wood frog skin micromolecule III type collagen peptide. The enzymolysis preparation process comprises the following steps: mixing defatted wood frog skin powder with water, and pretreating; adding a papain-neutral protease-glutamine protease hydrolysis system into the pretreated mixed solution, continuously hydrolyzing, performing centrifugal treatment after the hydrolysis is completed, heating supernate, adding alkaline protease into the supernate for hydrolysis and enzyme inactivation, and performing centrifugal treatment to obtain clear liquid rich in collagen peptide; carrying out multistage membrane separation coupling chromatography purification treatment and low-temperature vacuum concentration treatment on the clear liquid rich in collagen peptide to obtain a concentrated solution; and mixing the concentrated solution with a trehalose-hyaluronic acid composite wall material, and performing spray drying to obtain the collagen peptide nanocapsule. Efficient preparation and function enhancement of the wood frog skin type III collagen peptide can be achieved, and the prepared collagen peptide has the purity larger than or equal to 99.5% and the activity retention rate larger than 98% and is excellent in heat stability and moisturizing performance.

The invention provides a recombinant III-type collagen with a triple helix structure as well as a preparation method and application thereof, and relates to the field of protein engineering. According to the invention, a novel humanized III-type collagen functional fragment with biological activity is obtained through screening by selecting low-immunogenicity and high-biological activity blocks after complete sequence analysis of human III-type collagen. The functional fragments obtained by screening are repeatedly combined to obtain the novel recombinant humanized III-type collagen, and the recombinant humanized III-type collagen is a novel protein with strong biological activity and low immunogenicity, and provides a novel raw material for medical instruments and skin care products. The recombinant humanized III-type collagen sequence is efficiently expressed by adopting engineering bacteria, the expression level is high, the purification method is simple, and the recombinant humanized III-type collagen sequence is suitable for industrial production and wide popularization.

The invention belongs to the technical field of natural product extraction, and particularly relates to a vaccinium macrocarpum fruit extract-containing composition which comprises the following main components in percentage by mass: 0.04-0.3% of vaccinium macrocarpum nanoemulsion, 1-5% of vaccinium macrocarpum seed oil, 0.05-10% of III-type collagen, 1-10% of multiple hyaluronic acid, 0.5-2% of rosmarinic acid and the balance of water. The preparation method of the composition containing the vaccinium macrocarpum fruit extract comprises the following steps: step 1, selecting fresh vaccinium macrocarpum fruits, performing quick freezing, performing vacuum freeze drying until the water content is less than or equal to 5%, performing crushing, and performing sieving with a 80-mesh sieve; 2, 200 mL of a citric acid buffer solution with the pH being 4.0 is added into every 100 g of fruit powder, 0.6% of compound enzyme is added, and stirring and activating are conducted for 30 min at the temperature of 40 DEG C; step 3, adding 60% acidified ethanol according to a solid-liquid ratio of 1: 3, and performing ultrasonic extraction at 50 DEG C (200W) for 1.5 hours; according to the composition disclosed by the invention, through the innovation of extraction, purification, vectorization and freeze-drying full-chain technologies, the bottlenecks of large activity loss and low transdermal rate of a traditional process are broken through, and the synergistic effect of triple effects of oxidation resistance, wrinkle resistance and barrier repair is realized.

The invention relates to a recombinant vector for expressing a natural long-sequence recombinant III-type collagen as well as a preparation method and application of the recombinant vector, and relates to the field of proteins. The nucleotide sequence for optionally coding the recombinant III type collagen has at least one section of nucleotide sequence for coding the amino acid sequence as shown in SEQ ID NO: 1 (optionally has at least one section of nucleotide sequence as shown in any one of SEQ ID NO: 2 to SEQ ID NO: 4). The engineering bacterium for efficiently expressing the III type collagen natural long sequence is constructed through a genetic engineering technology, large-scale production of the engineering bacterium is achieved, and the engineering bacterium can be widely applied to the fields of medicine, clinical skin repair, tissue engineering, beauty and the like.

The present invention discloses a recombinant type III collagen with high thermal stability and high transdermal absorption and its application, belonging to the technical field of genetic engineering drugs. The core amino acid sequence of a secretion peptide is inserted upstream of the collagen active fragment gene, and a purification tag is added downstream of the collagen active fragment gene; then this gene sequence is subjected to protein expression and purification in a host bacterium; the amino acid sequence of the collagen active fragment is as shown in SEQ ID NO.3; the core amino acid sequence of the secretion peptide is as shown in SEQ ID NO.2. The amino acid sequence of the recombinant type III collagen with high thermal stability and high transdermal absorption is as shown in SEQ ID NO.1. By the above method, the recombinant type III collagen containing the secretion peptide of the present invention overcomes the poor absorbability of natural collagen, shows a higher transdermal absorption effect, and has high thermal stability.

The invention provides a composition containing recombinant collagen with a triple helix structure, and belongs to the technical field of biology. The recombinant humanized III type collagen and the recombinant humanized XVII type collagen in the composition can synergistically promote cell adhesion, promote collagen regeneration, up-regulate expression of genes related to skin hydration and down-regulate expression of inflammatory factor genes, and can be applied to products for resisting aging, tightening, moisturizing, repairing and / or relieving inflammation.

1. Name of the Design Product: Packaging Box (Type III Collagen Mask). 2. Use of the Design Product: For product packaging. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Three-dimensional view.

The invention discloses a recombinant III-type collagen and PDRN compound composition and a preparation method thereof, and relates to the technical field of biological medicines. The composition is formed by compounding recombinant human III type collagen and polydeoxyribonucleotide according to the mass ratio of 1: 10-10: 1, the weight-average molecular weight of the recombinant human III type collagen and the weight-average molecular weight of the polydeoxyribonucleotide are 50-100 kDa, the ratio can generate synergistic interaction, fibroblast proliferation, migration and collagen secretion are remarkably promoted, and the activity of the composition is superior to that of a single component and commercially available products. The composition can be used for preparing medical instruments, cosmetics or water-light injections for skin repair, rejuvenation or skin improvement. The invention also provides a water-light injection containing the compound composition, and the obtained preparation is stable, clear, transparent, safe and non-irritant.

The invention belongs to the technical field of skin care products, and relates to full-layer anti-aging recombinant collagen filling powder as well as a preparation method and application thereof. The recombinant collagen filling powder is prepared from recombinant I type collagen, recombinant III type collagen, recombinant IV type collagen, recombinant VI type collagen, recombinant XVII type collagen, superoxide dismutase, a bifidus yeast lysate, trehalose, silica, silica dimethyl silylate and a polydimethylsiloxane / vinyl polydimethylsiloxane cross-linked polymer, and the recombinant collagen filling powder is prepared from the following raw materials: recombinant I type collagen, recombinant III type collagen, recombinant IV type collagen, recombinant VI type collagen, recombinant XVII type collagen, superoxide dismutase, bifidus yeast lysate, trehalose, silica, silica dimethyl silylate and a polydimethylsiloxane / vinyl polydimethylsiloxane cross-linked polymer. The skin care product is prepared from glycerin, xanthan gum, sodium hyaluronate, dimeticone, phenoxyethanol and ethylhexylglycerin. The recombinant collagen filling powder provided by the invention is convenient to use, can obviously improve the skin moisturizing capacity, improve the compactness and repair wrinkles, has a good anti-aging effect and a good repair effect, and has important practical significance and market value.

The invention belongs to the technical field of biomedical materials, and particularly relates to nucleic acid for synthesizing human III-type collagen as well as an in-vivo expression method and application of the nucleic acid. The nucleic acid sequence of the synthesized human III type collagen is as shown in SEQ ID NO. 1. The invention provides an effective mRNA (messenger ribonucleic acid) nucleic acid, the nucleic acid encodes a human full-length III-type collagen gene sequence, can effectively realize expression in a human body, and is guided to translate into full-length III-type collagen through the nucleic acid in human cytoplasm and has a correctly folded space structure triple-helix collagen, namely human III-type collagen.