49 results about "Type III collagen" patented technology

The invention discloses a composition with an anti-aging function and a preparation method thereof as well as application of the composition with the anti-aging function in the field of skin care products. The composition with the anti-aging function is also called a biodynamic rejuvenating complex factor. The composition with the anti-aging function is prepared from the following raw materials in parts by weight: 1-50 parts of beluga caviar extract, 1-30 parts of GPPPPG, 1-2 parts of wild soybean protein, 0.1-5 parts of oxidordeuctase and 0.1-15 parts of palmitoyl oligopeptide and palmitoyl tetrapeptide-7. The composition with the anti-aging function can be used for effectively increasing content of type I collagen and type III collagen in skin, and the effects of reducing wrinkles, reducing loose skin and reducing skin roughness are realized; and the composition with the anti-aging function can be used for inhibiting decomposition of elastic fibers, promoting generation of elastic proteins in skin, enhancing skin elasticity and reducing damage of free radicals and ultraviolet rays (UV) on skin, and the effect of delaying skin senility is achieved. The composition with the anti-aging function can be widely applied to whitening, anti-aging, sunscreen and moisturizing nutrition-based cosmetics in dosage forms such as cream, emulsion, aqueous solution, hair gel and facial mask.

The invention discloses a preparation method of a cell preparation for promoting the regeneration and the repair of traumas. The preparation method comprises the steps of shearing and digesting heel tendon tissues, carrying out centrifuging to remove supernate, adding an obtained tendon stem cell population into a culture solution, carrying out resuspension and filtering, inoculating the culture solution into a culture plate for culturing, mixing the culture solution with platelet rich plasma, and adding normal saline, so as to prepare a tendon stem cell population. Tendon stem cells are separated and cultured, tendon stem cells cultured in vitro are mixed with the platelet rich plasma to prepare the cell preparation, the platelet rich plasma contains a large number of growth factors such as PDGF, EGF, TGF-beta1 and IGF which have great promotion effects to the repair of tendons, the platelet rich plasma is capable of promoting the propagation of tendon cells and the expression of collagens I and III in vivo, and the tendon cells and the collagens I and III are key components of the tendons, so that an experimental result has a certain guiding effect to the repair of tendon injury of a human body.

The invention relates to a chronic articular inflammation-modulating composition which uses collagen-polyvinylpyrrolidone. Collagen turnover is modulated using collagen-PVP in patients with rheumatoid arthritis and osteoarthritis, since the biocomplex negatively regulates collagenolytic activity and increases the content of type III collagen and TIMP-1 to levels similar to those observed in normal synovia. In this way, the chronic inflammatory process is altered by the collagen-PVP, as described above, owing to the negative regulation of IL-1β, the TNF-α of IL-8 and the adhesion molecules, since said molecules can induce the expression and activation of collagenolytic enzymes, as well as the proliferation and migration of synovial cells by means of the adhesive mechanisms including the adhesion molecules, and the induction and activation of Cox-1. In addition to cellular anchoring, the interaction of the extracellular matrix integrins generates signals which regulate the expression of M MPs, suggesting that the adhesion molecules can also play a part in tissue destruction associated with rheumatoid arthritis. Moreover, the decrease in the synthesis of TNF-α and IL-1β appears to stimulate the cell death of synoviocytes by means of apoptosis, which, in turn, seems to contribute to the inhibition of the proliferation of synovial cells, the formation of pannus and the destruction of the articular tissue.

The invention discloses an application of glycosyl modified polyphenol compounds as drugs for relieving paraquat poisoning and treating pulmonary fibrosis. The glycosyl modified polyphenol compounds have the structure shown in the description. The glycosyl modified polyphenol compounds have a good oxidation resistance function and a lung protection function and have a notable protection function on lung injury caused by paraquat, and a mechanism is related with reduction of formation of oxygen radicals, relieving of oxidative stress and inflammatory reaction, increase of content of GSH in blood serum and SOD in lung tissue and reduction of content of MDA and HYP in lung tissue. The glycosyl modified polyphenol compounds can effectively inhibit bleomycin-induced pulmonary fibrosis of mice,and the mechanism is related with reduction of expression of TGF-beta 1, IL-6, alpha-SMA, P-smad2, type I collagen and type III collagen in lung tissue, increase of content of GSH and SOD in blood serum and reduction of the content of HYP in lung tissue.

The invention discloses an application of Eucommia lignans in preparing medicaments for preventing and treating hypertension-induced renal injury. The Eucommia lignans are extracted from Eucommia barks as raw materials and used to prepare medicaments for preventing and treating hypertension-induced renal injury. The Eucommia lignans can better improve the damage of the renal glomeruli and tubules due to spontaneous hypertension and have the pharmacological action of inhibiting the expression of type III collagen and the proliferation of mesangial cells, so as to effectively prevent and treat hypertension-induced renal injury.

The invention discloses a fermentation method and fermentation medium for recombinant human III type collagen engineering bacteria and belongs to the technical field of engineering bacteria fermentation. The fermentation method comprises the following steps that the recombinant human III type collagen engineering bacteria are inoculated to a basic fermentation culture solution to conduct fermental cultivation, and the basic fermentation culture solution comprises, by liter, 25-27 ml of H3PO4, 0.8-1.0 g of CaSO4, 17-19 g of K2SO4, 13-15.5 g of MgSO4.7H2O, 3.8-4.3 g of KOH, 37-43 g of glycerinum and 3.5-4.2 ml of a first microelement solution; and in the fermental cultivation process, supplementary materials containing glycerinum and a second microelement solution are added into the basic fermentation culture solution. By optimizing the basic fermentation culture solution and the component content of the supplementary materials, the protein expressing quantity of the recombinant human III type collagen engineering bacteria is greatly improved by the fermentation method, and the method can be applicable to industrial large scale production.

The invention discloses a method for extracting water-soluble collagen. The method comprises the following steps: firstly slicing a tissue rich in type III collagen; then cleaning tissue slices and soaking in 0.1-40%(W/V) alkali solution for 0.5-24 hours; cleaning until pH is 5-8, then soaking for about 0.5-48 hours with normal saline, draining excessive moisture, mashing the tissue at the temperature of 2-8 DEG C, then dispersing into enzymatic hydrolysate, and carrying out enzymatic hydrolysis for 1-72 hours in an environment at the temperature of 2-40 DEG C; after the enzymatic hydrolysis is finished, regulating pH value to be 7-13, and standing for 2-24 hours at the temperature of 2-40 DEG C; filtering with a filter screen of 30-150 meshes, and removing substances which are not subjected to enzymatic hydrolysis; salting out with 20-50%(W/V) salt solution, collecting solid contents, carrying out dialysis with a dialysis bag with the molecular weight cut-off of 4000-8000, and removing excessive ions and impurities, so that polymorphic collagen is obtained; and finally, dispersing a polymorphic collagen mixture into water, regulating pH value to 1-5, filtering with a filter screen of 30-150 meshes, removing precipitates, salting out filtrate, and then carrying out dialysis, so that the target collagen is obtained. By adopting the method for extracting the water-soluble collagen, the obtained collagen is an aqueous solution, and the blank that existing collagen is acid soluble or alkali soluble is filled up.

The invention discloses a skin care product for promoting RNA transcription. The skin care product comprises the following components in parts by mass: 2.3 to 5.1 parts of triglyceride decanoate, 4 to6 parts of squalane, 2 to 3.6 parts of ethylhexyl palmitate, 5 to 7.3 parts of isopropyl myristate, 3.2 to 4.4 parts of mink oil, 1.3 to 3.3 parts of PEG-7 glyceryl cocoate, 20 to 40.8 parts of deionized water, 5 to 7 parts of seaweed oligosaccharin, 7 to 10 parts of glycerol, 2.3 to 3.6 parts of microorganism C, 5.5 to 8.6 parts of retinol, 3.8 to 6.5 parts of peptide, 3.3 to 4.5 parts of coenzyme Q10, 20 to 30 parts of recombinant human III type human-like collagen and 1.5 to 3.4 parts of interleukin 13. The invention further provides a preparation process of the skin care product for promoting RNA transcription. A preparation method comprises the following steps of S1, selecting 2.3 to 5.1 parts of triglyceride decanoate, 4 to 6 parts of squalane, 2 to 3.6 parts of ethylhexyl palmitate, 5 to 7.3 parts of isopropyl myristate, 3.2 to 4.4 parts of mink oil and 1.3 to 3.3 parts of PEG-7 glyceryl cocoate, and starting steam for heating; S2; S3; S4; and S5. The skin care product is reasonable in structure; transcription of type III collagen genes can be accelerated; the higher the transcription number is, the more generated messenger RNA is; and the content of skin collagen is greatly increased accordingly.

The invention relates to the technical field of skin care products, in particular to a polypeptide composition capable of resisting ageing. By parts by weight, the polypeptide composition at least comprises 0.2-0.7 parts of moisturizing agent A, 0.03-0.35 parts of active polypeptide and 0.5-2 parts of swertiamarin. The polypeptide composition can make skin have real functions of moisture locking and moisture preservation, keeps the skin elastic and glossy, and meanwhile, can stimulate skin cells to synthesize more type III collagen, and thereby, skin ageing is prevented.

The invention provides a medical collagen oral ulcer protection gel and a preparation method thereof, and relates to the technical field of medical preparations. The medical collagen oral ulcer protection gel comprises the following raw materials: type III collagen, type I collagen, an excipient, and a preservative. The medical collagen oral ulcer protection gel has low medicine odor, and little irritation to the oral mucosa, and is used to relieve pains caused by wounds caused by oral ulcers, oral inflammation, dentures or surgeries, the wound healing is promoted, the curative effect is significant, fast anti-inflammatory and pain-relieving can be achieved, the use is simple, and thereby the protection gel has wide applicability and commercial value.

The invention discloses an injection filling material and a preparation process. The filling material is prepared from the following raw materials in percentage by mass: 0.1-2% of sodium hyaluronate,1-30% of polyester microspheres, 0.1-5% of silk fibroin, 0.01-1% of recombinant III-type collagen and the balance of dispersion liquid. The polyester material is artificially synthesized, the sodium hyaluronate and the recombinant protein are prepared through a fermentation method, the silk fibroin is extracted from secretions of silkworms instead of animal tissues, and compared with collagen extracted from the animal tissues, the acellular matrix has better biological safety; the added polyester microspheres can form a slow release effect on the system, the effective time of the filling material is prolonged, and the lasting time of the filling material is longer than that of a single-component material; and the filling material comprises type I collagen (silk fibroin), type III collagen(recombinant protein) and carbohydrate nutrient substances (hyaluronic acid) required by tissue production, and a complete external environment can be provided for tissue production.

The invention relates to the field of health care products, and in particular, relates to an extract, and a preparation method and an application thereof. Safflower and radix salviae miltiorrhizae arebiotransformed by ganoderma lucidum, an extract obtained by extracting an obtained fermentation product can inhibit the production of beta-galactosidase in aging fibroblasts, improves the gene expression of type-I collagen and type-III collagen in an aging process of the fibroblasts, and has the effect of resisting skin aging; the effects are proved significantly (p<0.05) to be better than thoseof conventional plant extracts or a combination thereof.

The invention belongs to the technical field of stem cell culture, and particularly relates to an adipose-derived stem cell serum-free culture medium and a preparation method thereof. The adipose-derived stem cell serum-free culture medium provided by the invention comprises a basal culture medium, beta globulin, L-arginine, a bactericide, type III collagen and a cell attachment factor. The adipose-derived stem cell serum-free culture medium provided by the invention can prolong the liquid change time, is high in cell collection rate, is high in purity of finally cultured cells, contains few components, reduces the production cost, and is convenient for subsequent deep research on adipose-derived stem cells.

The invention relates to a biochemical marker for CVD risk evaluation and a method of bioassay for the quantification of peptide fragments comprising a neo-epitope formed by cleavage of a protein of an atherosclerotic plaque such as lumican, versican, perlecan, decorin, biglycan, collagen type III, CRP, ApoE, or elastin, by a proteinase, said comprises contacting a sample such as urine or serum with an antibody reactive with the neo-epitope and determining the level of binding of said immunological binding partner to peptide fragments in said sample. The assay is predictive of risk of cardiovascular disease events.

The invention relates to the technical field of a preparation method of a bioactive substance, in particular to a method for preparing animal-derived extracellular matrix polypeptide by using a physical method. The method comprises the following steps: 1 cleaning, cutting and carrying out high-temperature treatment on animal-derived tissue to obtain a tissue sample; 2 carrying out deep freezing treatment on the tissue sample, and thawing the tissue sample with water at 25-35 DEG C, and circulating the process for 3-5 times to obtain a thawed tissue sample; 3 physically crushing the thawed tissue sample to obtain powder particles; 4 dissolving the powder particles in water, and performing high-speed stirring and emulsification to obtain slurry; 5 heating the slurry for dissolution, carryingout suction filtration, collecting a filtrate, and performing drying to obtain powdery semi-finished product; and 6 carrying out irradiation treatment on the powdery semi-finished product to obtain the animal-derived extracellular matrix polypeptide. The ultralow-molecular-weight sterile complex polypeptide rich in type III collagen sequences is prepared with a combined physical preparation method, and high bioactive components are provided for skin whitening, caring and repair.

The invention relates to the technical field of bioengineering, in particular to a hernia repair material compounded with adipose-derived mesenchymal stem cells and gelatin sponge as well as a preparation method and application of the hernia repair material. The material comprises a biological patch and gelatin sponge on the lower side of the biological patch, and the gelatin sponge is filled with adipose-derived mesenchymal stem cells; the biological patch is of a natural fiber structure mainly composed of type I and type III collagen, and the number of adipose-derived mesenchymal stem cells in the gelatin sponge is 1 * 10 < 6 > to 3 * 10 < 6 >/cm < 2 >. After the gelatin sponge filled with the adipose-derived mesenchymal stem cells is compounded with the biological patch, the survival rate of the adipose-derived mesenchymal stem cells is increased, the degradation speed of the biological patch is delayed, wound healing is accelerated, muscle-like tissues are formed in vivo, the strength of the biological patch is enhanced, and the purpose of better treating abdominal wall soft tissue defect (hernia) is achieved.

The invention discloses a method for constructing a type III collagen photoelectrochemical sensor based on a rhenium disulfide nanosheet and an application. The method comprises the following steps offirstly, preparing 1-butyl-3-dodecyl imidazole bromide salt ionic liquid; taking the ionic liquid as an auxiliary reagent; successfully manufacturing a rhenium disulfide two-dimensional lamellar nano-material by using probe ultrasonic stripping; and constructing the type III collagen photoelectrochemical sensor on the basis of a rhenium disulfide nano-sheet modified electrode and loading of a type III collagen antibody, and realizing specific detection of type III collagen by taking white light as an excitation light source. In the invention, the ionic liquid is used as a stripping agent to be inserted into or attached to a nano lamellar structure of rhenium disulfide so that rhenium disulfide nanosheet agglomeration can be effectively avoided, and stripping efficiency is improved; and rhenium disulfide belongs to a direct band gap material, and has a better photoelectric property than other transition metal disulphides so that high-selectivity and high-sensitivity analysis of the type III collagen is realized.