107 results about "Type III collagen" patented technology

The invention relates to the technical field of biology, and particularly discloses a recombinant collagen III and application thereof in preparation of gel. The recombinant collagen III is designed by optimizing functional area sequences of human I-type and III-type collagen, the amino acid sequence is shown as SEQ ID No.2, and the recombinant collagen III has the characteristics of high stability, good hydrophilicity and low immunogenicity. The preparation method comprises the steps of expression vector construction, escherichia coli induced expression, affinity chromatography purification and renaturation. The recombinant collagen III can be prepared into a gel dressing and comprises sodium alginate, methylparaben and other components. Experiments show that the gel can effectively promote cell proliferation and has no cytotoxicity; in a mouse skin injury model, the collagen can relieve inflammation, accelerate wound healing and inhibit scar formation, and the effect of the collagen is superior to that of natural human III-type collagen. The invention provides a safe and efficient novel material for wound repair, and is suitable for medical dressings and tissue engineering.

The present invention relates to the technical field of protein engineering and provides a recombinant humanized type III collagen having a triple helix structure, a preparation method therefor, and a use thereof. The amino acid sequence of the recombinant humanized type III collagen comprises n core units, wherein n is 1 or an integer greater than 1, and the amino acid sequence of the core unit is as shown in SEQ ID NO: 1. The present invention further provides a method for constructing an expression system and a method for preparing a recombinant humanized type III collagen. The present invention further provides an actual industrial use on the basis of the characteristics of the protein. The recombinant humanized type III collagen provided by the present invention has high purity, good stability, and high biological activity. Experiments have shown that the recombinant humanized type III collagen has various biological activities such as promoting cell proliferation and migration.

The application discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application thereof, and belongs to the technical field of cosmetics and skin care products. The composition contains the following active ingredients in percentage by mass: 0.01%-20% of recombinant humanized type I collagen, 0.01%-20% of recombinant humanized type III collagen, 0.01%-10% of recombinant humanized type XVII collagen, and 0.01%-20% of space-bred desert algae extract. The three kinds of recombinant humanized collagens and the space-bred desert algae extract form a complex system, and when the total mass ratio is in a specific interval of 1.3:1-4:1, the three kinds of recombinant humanized collagens and the space-bred desert algae extract can synergistically improve the skin barrier function. Based on the synergistic mechanism of the complex system, the composition disclosed by the application can effectively promote the skin moisture retention and anti-wrinkle ability, repair the skin barrier, and is suitable for the development and application of skin care products for anti-wrinkle, skin tightening and skin aging delay.

The invention discloses a freeze-dried essence with a repairing effect and a preparation method, and belongs to the field of skin care products, and the freeze-dried essence is composed of freeze-dried powder and an independent solvent liquid. The freeze-dried powder contains 0.5-3 parts of palmitoyl oligopeptide, 3-8 parts of I-type collagen, 1-5 parts of III-type collagen, 0.1-1 part of chitosan, 1-3 parts of secondary variable-temperature fermentation liquor of betula platyphylla and 2-8 parts of a freeze-drying protective agent; the solvent liquid is prepared from 0.5 to 3 parts of hyaluronic acid and 0.05 to 0.5 part of sodium polyglutamate. The preparation method comprises the following steps: a, adding fermentation liquor after dissolving; b, adding nano liposome and a protective agent; c, quickly freezing to-55 + / -2 DEG C by liquid nitrogen, and preserving heat for 2.5-3.5 hours; d, drying for 9-11 hours under the vacuum of 15-25 Pa and at the temperature of-18 + / -2 DEG C; and e, the temperature is increased to 15 + / -2 DEG C in a stepped mode, vacuum is smaller than or equal to 20 Pa, and drying is conducted for 18- Through scientific formula design, multiple effects of efficient repairing, anti-aging and relieving are achieved.

The invention provides a medical-grade recombinant three-type collagen and application thereof. The recombinant humanized three-type collagen has certain cell proliferation promoting capacity and is free of cytotoxicity. The medical-grade recombinant three-type collagen provided by the invention not only has the capability of promoting cell proliferation, is free of cytotoxicity and high in cell adhesion activity, but also has a three-helix structure characterized by the collagen and is extremely high in stability, the purity can reach about 85% after the collagen is induced for 120H in a 100L fermentation tank, the difficulty of a downstream purification process is greatly reduced, and the production cost is reduced. The effect in the aspect of cell activity is obvious.

The invention discloses a recombinant III-type collagen and PDRN compound composition and a preparation method thereof, and relates to the technical field of biological medicines. The composition is formed by compounding recombinant human III type collagen and polydeoxyribonucleotide according to the mass ratio of 1: 10-10: 1, the weight-average molecular weight of the recombinant human III type collagen and the weight-average molecular weight of the polydeoxyribonucleotide are 50-100 kDa, the ratio can generate synergistic interaction, fibroblast proliferation, migration and collagen secretion are remarkably promoted, and the activity of the composition is superior to that of a single component and commercially available products. The composition can be used for preparing medical instruments, cosmetics or water-light injections for skin repair, rejuvenation or skin improvement. The invention also provides a water-light injection containing the compound composition, and the obtained preparation is stable, clear, transparent, safe and non-irritant.

The invention belongs to the technical field of gene engineering, and provides a high-solubility and high-transdermal-property type III collagen as well as a preparation method and application thereof, and the amino acid sequence of the type III collagen is as shown in SEQ ID NO.1; and the N end of the III-type collagen is connected with a cell-penetrating peptide. The collagen provided by the invention has high dissolvability and high transdermal property, and also has relatively good tightening and moisturizing properties.

The invention provides a collagen nano dispersion preparation as well as a preparation method and application thereof. The preparation method of the collagen nano dispersion preparation comprises the following steps: S1, dissolving type I collagen and type III collagen in an acidic aqueous medium to prepare a collagen solution; s2, regulating and controlling the temperature, the pH value and the ionic strength of the collagen solution, so that the collagen solution is self-assembled; and S3, when the average particle size of the collagen fibers reaches a target range, performing dynamic freezing on the self-assembly system. The preparation and stable dispersion of the nano-scale collagen fiber can be realized without chemical crosslinking, the uniformity, biocompatibility and safety of the product are remarkably improved, and the nano-scale collagen fiber has excellent water-light effects and the like, and can be widely applied to the field of medical cosmetology.

This invention discloses recombinant type III collagen, its preparation method, and its applications. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID NO: 9. The recombinant type III collagen integrates the RGDV highly active motif and the EKEK hydrophilic stable domain with the core structure of type III collagen to achieve an integrated design of "high activity and long-lasting effect". The in vitro cell migration and collagen secretion effects are improved by more than 50% compared with wild type. Through anti-enzyme cleavage site modification and hydrophilic modification, the in vivo residence half-life is significantly prolonged to meet the long-term repair needs of the dermis.

The invention specifically discloses a fermentation process suitable for III-type collagen expression, and relates to the technical field of biological fermentation of recombinant genetic engineering bacteria. According to the method disclosed by the invention, a double-carbon-source mixed feeding strategy of alanine and methanol is used, so that the problems that when pichia pastoris expresses exogenous target protein by taking methanol as a unique carbon source and energy source, cell growth and protein expression fight for the carbon source and the energy source together, the metabolic burden of cells is increased, and the expression efficiency is low are solved; through a three-step methanol fed-batch strategy, the expression efficiency is improved, and the toxic action of methanol on cells is reduced, so that the yield of the III-type collagen is further improved. The fermentation process provided by the invention solves the problem of reducing the production cost while improving the high-efficiency and high-quality expression of the target product under the condition of high-density thallus fermentation.

The present application relates to the field of biotechnology, in particular to the application of Radix Rabdosiae Rubescens derived exosomes in the preparation of a drug for promoting wound healing, wherein the prepared Radix Rabdosiae Rubescens derived exosomes are uniform in size, good in appearance, can promote cell proliferation, have good effects of promoting fibroblast migration and reducing scar formation; and a scar-free wound healing hydrogel containing Radix Rabdosiae Rubescens derived exosomes is also provided, wherein the hydrogel can encapsulate rEVs or ORI, can effectively increase the proportion of type III collagen in the new skin tissue, effectively reduce the formation of scars, effectively promote the healing of deep second-degree burn wounds, and effectively inhibit the MMT process of macrophages in the wound healing process, and has a wide application prospect in the field of scar prevention and treatment.

The invention discloses an application of hsa-miR-17-3p in the prevention and / or treatment of hepatic fibrosis. Through clinical sample sequencing, it is found for the first time that expression of hsa-miR-17-3p (SEQ ID NO.1) in hepatitis B related hepatic fibrosis tissue is remarkably reduced. Furthermore, a dual luciferase reporter gene experiment proves that the hsa-miR-17-3p can be directly combined with a 3'untranslated region of the TGFBR1 in a targeting manner to inhibit mRNA and protein expression of the TGFBR1, so that activation of hepatic stellate cells is effectively inhibited, and secretion of I-type and III-type collagen is remarkably reduced. The discovery not only reveals a new mechanism of hepatic fibrosis, but also provides a theoretical basis and candidate molecules for developing novel targeted therapeutic drugs based on miRNA, and has important clinical application value and wide prospects in the aspects of early intervention, treatment and diagnosis of hepatic fibrosis.

This invention discloses recombinant humanized type III collagen, its preparation method, and its applications, belonging to the field of medical materials technology. The amino acid sequence of this recombinant humanized type III collagen is any one of SEQ ID No. 2-6. Compared to traditional extraction methods, the amino acid sequence of this recombinant protein is highly consistent with that of natural human proteins, completely avoiding the biosafety risks of pathogens, prions, etc., that may exist during animal-derived extraction, while also exhibiting lower immunogenicity. Furthermore, this protein effectively maintains the natural biological activity and stability of collagen. When used as a wound dressing, this material can efficiently promote cell migration, spreading, and proliferation, accelerate tissue regeneration and wound healing, while possessing excellent biocompatibility, controllable degradation, and low immunogenicity.

The invention discloses an application of an engineered mRNA (messenger Ribonucleic Acid) medicine for coding COL3A1 protein in resisting skin photoaging. According to the method, accurate rational design and optimization are carried out on the 5'untranslated region (UTR) of the hCOL3A1 mRNA, so that the translation efficiency and the protein yield of the hCOL3A1 mRNA are greatly improved, the expression level of the hCOL3A1 target protein far beyond the conventional sequence in vivo and in vitro is realized, and the type III collagen is efficiently and endogenously synthesized in skin cells. Experimental results show that the engineered mRNA can effectively reduce the skin oxidative stress level, inhibit cell aging and promote endogenous synthesis and deposition of corium layer collagen, so that the barrier function of photoaged skin is remarkably improved, the dermis thickness is increased, the tissue structure is repaired, and systemic toxicity or immune side effects are not observed. The invention provides a novel strategy with high efficiency and safety for resisting skin photoaging.

The invention belongs to the technical field of recombinant collagen sequence analysis, and discloses a recombinant collagen sequence analysis method based on AI deep learning. Comprising the following steps: extracting a natural III-type collagen sequence; applying the natural III-type collagen sequence to the trained model to generate a thermodynamic diagram; generating an optimized natural III type collagen sequence based on the thermodynamic diagram; performing fine tuning on the AlphaFold2 model by using the fine tuning data set, and obtaining an integrated structure model by introducing spiral torsion constraint and hydroxyproline simulation parameters; loading the optimized natural III-type collagen sequence, and predicting the stability of the triple helix structure of the sequence through the structure model; and extracting a natural III-type collagen sequence which passes the stability detection, and outputting a report file by using a skin permeability prediction model to realize recombinant collagen sequence analysis based on AI deep learning.

This invention belongs to the field of biomedical materials and skin / hair repair technology, specifically a recombinant collagen freeze-dried composition targeting hair follicle functional sites, its preparation method, and its application. The composition includes recombinant human collagen, selected from recombinant type III collagen containing a Wnt-binding domain, recombinant type XVII collagen containing an NC16A domain, or a combination thereof; a freeze-drying protectant composed of trehalose and mannitol in a mass ratio of 1:2 to 2:1; and a buffer system, a phosphate buffer with a pH of 6.5-7.5 and a concentration of 5-20 mM. This invention provides precise hair follicle targeting and efficacy far exceeding that of ordinary collagen. It is effective for androgenetic alopecia, telogen effluvium, and diffuse thinning hair loss. It can be used for individuals with scalp micro-damage and sensitive scalps, simultaneously improving the scalp microenvironment and preventing hair loss from the root. Furthermore, its long-term stable effects have been verified over multiple time points.

The application provides an engineered exosome loaded with a CD63 transmembrane domain-matrix metalloproteinase fusion protein and a preparation method and application thereof. The engineered exosome is secreted by a genetically engineered mesenchymal stem cell, has a CD63 derived transmembrane domain anchored on the membrane, and has a matrix metalloproteinase (MMP) loaded on the inner side of the membrane. The fusion protein comprises, from N-terminus to C-terminus, a fourth transmembrane domain and a C-terminal cytoplasmic tail of a human CD63 protein, a flexible linker peptide, and a human MMP without a natural signal peptide. The application realizes efficient and directional loading of MMPs by using a natural biological generation path of the exosome, shields the inhibition of MMPs by metalloproteinase tissue inhibitors in a scar tissue by using the membrane of the exosome, realizes specific activation of MMPs in a target cell, degrades abnormally deposited collagen, adjusts the ratio of type I and type III collagens, and plays an anti-fibrosis and anti-scar formation role. The application also provides a preparation method of the engineered exosome and application thereof in preparation of a medicine for preventing or treating pathological scars.

The invention relates to a moisturizing and repairing medical spray containing glycosylglycerol and a preparation method of the moisturizing and repairing medical spray. The anti-inflammatory and anti-inflammatory gel is prepared from the following components: 0.5%-2% of glycosylglycerol (wherein the mass ratio of 2-beta GG to 2-alpha GG is 0.08: 1), 0.01%-0.5% of recombinant humanized III-type collagen, 0.3%-0.7% of trehalose, 0.05%-0.3% of beta-glucan (wherein the molecular weight ratio is specific), 3%-10% of a humectant, 0.1%-1% of a soothing anti-inflammatory agent, 0.01%-0.5% of a pH regulator, 0.01%-0.3% of a preservative and the balance of deionized water. Wherein the four active components meet a specific relational expression. The invention also provides a preparation method and application of the medical spray. According to the medical spray disclosed by the invention, through the specific proportion and synergistic matching of the active ingredients, excellent moisturizing performance, barrier repairing function, wound healing activity and soothing and anti-inflammatory effects are realized, and the medical spray is high in safety, good in use experience and suitable for nursing of various skin types, particularly sensitive skin and damaged skin.

The invention relates to the technical field of high-yield collagen MSC (mesenchymal stem cell) engineering construction, and particularly discloses a fixed-point controllable high-yield collagen MSC engineering construction method, which comprises the following steps: 1, MSC separation and primary culture: separating from mammal bone marrow, umbilical cord or adipose tissue to obtain primary MSC, adopting a DMEM / F12 culture medium containing 13% of fetal calf serum, and carrying out primary culture on the primary MSC; carrying out primary culture and passage in a culture environment of 37 DEG C and 5% CO, and screening the fourth generation of MSC in the logarithmic phase as an engineered starting cell; and 2, constructing a collagen synthesis related gene fixed-point editing carrier: designing a fixed-point editing element aiming at a collagen synthesis key gene and an MSC differentiation regulation gene. According to the site-specific controllable high-yield collagen MSC engineering construction method, the collagen expression quantity of the engineered MSC can be greatly improved, the directional synthesis proportion of the type I collagen and the type III collagen is improved, and the requirement of industrialization for high-yield and high-purity collagen is effectively met.

This invention belongs to the field of biomedical technology, specifically relating to a delivery system for type III collagen mRNA and its applications. The nucleic acid delivery system of this invention includes a delivery carrier and a delivered nucleic acid, wherein the nucleic acid is collagen mRNA, and the delivery carrier includes one or more of modified amino acids, growth factors, and polymeric materials. Through intradermal injection, the delivery system of this invention can be administered to the dermal layer of the skin and enter relevant cells, utilizing endogenous cellular proteins to achieve mRNA transfection, forming natural human type III collagen. This invention avoids the immunogenicity risks and structural inactivation problems associated with exogenous collagen injections, and can efficiently and safely replenish non-regenerative type III collagen in tissues. It can be used for various indications such as treating skin aging, promoting wound healing, reducing scar formation, and improving vascular elasticity.

This invention belongs to the field of cosmetic technology and discloses a skin care and hair care liquid composition of water-based polyurethane-10 and type III collagen. This invention combines water-based polyurethane-10 and type III collagen in a specific ratio, utilizing the residual NCO groups (or urea groups, carbamate groups) in polyurethane-10 to form a strong hydrogen bond cross-linking network with the amino and carboxyl groups in collagen molecules. This allows the collagen to firmly adhere to the skin or hair surface, while simultaneously softening the stratum corneum, resulting in a smooth skin feel and achieving multiple effects such as long-lasting moisturizing, improved elasticity, and wrinkle concealing.

The invention relates to the technical field of collagen, in particular to expression and application of recombinant humanized collagen. The recombinant collagen comprises AXBYCZ (AXBYCZ); wherein X and Z are independently selected from 0 or 1, and Y is an integer greater than or equal to 1; x and Z are not 0 at the same time; the structural domain A is a collagen N-terminal peptide or a peptide fragment thereof, the structural domain B is a Gly-Xaa-Yaa structure, and the structural domain C is a collagen C-terminal peptide or a peptide fragment thereof; the recombinant collagen not only shows excellent cell compatibility including relatively high cell viability, cell adhesion rate and cell proliferation rate, but also has the effects of resisting inflammation and inhibiting melanogenesis, and can promote synthesis of type I collagen, type III collagen and elastin, so that the recombinant collagen can be used for preparing the anti-inflammatory and anti-melanogenesis collagen. The method can be widely applied to the technical fields of biological medicine, medical equipment, cosmetics and biological engineering.

This invention relates to bioengineering, specifically disclosing a method for preparing recombinant humanized type III collagen with a triple-helix structure, comprising the following steps: S1, constructing a ppICZαA recombinant expression plasmid containing a human type III collagen fragment, and transforming the recombinant expression plasmid into yeast; S2, screening high-copy transformant strains from the strain and performing shake-flask level induction expression tests; S3, conducting high-density fermentation in a fermenter to obtain a large amount of secretible target protein. This invention, by constructing a ppICZαA recombinant expression plasmid containing a human type III collagen fragment and electroporating it into a yeast expression system, screens for yeast strains capable of expressing recombinant humanized type III collagen fragments, and conducts high-density fermentation in a fermenter, followed by purification after fermentation, yields pure recombinant collagen with a triple-helix structure, which can be applied in skincare products, functional skincare products, medical devices, and biomedical materials.

This invention provides a linear polypeptide and its application in cosmetics, belonging to the field of active molecule development technology. The polypeptide provided by this invention has a higher binding affinity to integrin receptors, significantly promoting fibroblast proliferation, increasing the content of elastin and type III collagen in the skin, and effectively scavenging DPPH free radicals and reducing reactive oxygen species levels. This polypeptide can be widely used in the preparation of cosmetics or topical skin formulations with anti-aging, antioxidant, and skin-repair promoting functions. It features high activity, good stability, and excellent safety, making it suitable as an active ingredient in functional skincare products.

The invention relates to a biological material loaded active bracket as well as a preparation method and application thereof. The human extracellular vesicles in the biological material-loaded active scaffold can regulate the functions of immune cells, inhibit the generation of inflammatory factors and relieve inflammatory response; the human extracellular matrix is rich in various collagens (such as type I collagen and type III collagen), elastin, fibronectin, sodium hyaluronate and other substances, has multi-dimensional advantages in the aspect of treating psoriasis, has the characteristic of being adaptive to the pathological mechanism of psoriasis, and can improve diseases from multiple levels; the human extracellular matrix and the hydrogel material can provide a good growth microenvironment for skin cells, promote normal proliferation and differentiation of keratinocytes, and recover normal structure and function of skin; the human extracellular vesicles and the human extracellular matrix have a synergistic effect, and psoriasis can be effectively treated.

The application discloses a fermentation process suitable for type III collagen expression, and relates to the technical field of recombinant genetic engineering bacteria fermentation. The application uses an alginine and methanol double carbon source mixed flow adding strategy, solves the problem that cells grow and protein expression jointly compete for carbon source and energy when Pichia pastoris expresses an exogenous target protein by taking methanol as the only carbon source and energy, increases the metabolic burden of the cells, and causes low expression efficiency; the three-step methanol flow adding strategy improves the expression efficiency and reduces the toxic effect of methanol on the cells, so that the yield of type III collagen is further improved. The fermentation process provided by the application solves the problem of improving high-efficiency and high-quality expression of a target product while reducing production cost under the condition of high-density bacterial body fermentation.

1.The name of the design product: packaging box (Wei Mei Hive Col 164.88° vertical box three). 2.The use of the design product: packaging box for injection of recombinant humanized type III collagen gel. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best indicates the design points: perspective view. 5.The design for which protection is sought contains color.

The invention relates to the technical field of preparation methods of cosmetic functional raw materials, in particular to application of a fructus arctii extraction product. The invention provides application of a fructus arctii extraction product in preparation of a product with the effect of improving the fiber structure order of a skin corium layer. According to the invention, a unique preparation process is adopted, and the active ingredients of phenolic acid and lignan substances are enriched, so that the expression of Fibulin-5, LOX, LOXL1 / 2 / 3, BMP1 and ADAMTS2 can be remarkably up-regulated by the fructus arctii extraction product, the ordered crosslinking of collagenous fibers is promoted, the activity of MMP-1 and MMP-12 is inhibited, the content of III-type collagen and elastin is increased, a multi-target and co-synergistic action mechanism is realized, and the effect of the fructus arctii extraction product is enhanced. Therefore, the potential of the fructus arctii extracted product for promoting ordered arrangement of fibers is improved, and reconstruction and reinforcement of a corium layer structure are realized on the molecular level.

This invention relates to recombinant mussel adhesive proteins, the nucleic acid molecules encoding them, nucleic acid constructs, expression vectors, recombinant engineered strains, pharmaceutical compositions, preparation methods, purification methods, and applications. This invention assembles and splices partial functional sequences from different mussels and type III collagen to obtain several novel mussel adhesive protein sequences. Further screening yielded recombinant mussel adhesive proteins Mfp5, Mfp55, Mfp131, and Mfp151, suitable for large-scale industrial production and exhibiting high expression levels. Efficacy experiments demonstrated that these proteins significantly promote cell migration, indicating their excellent cell repair function and broad application prospects.

The invention relates to a fusion protein, in particular, the fusion protein comprises the following elements fused together: (a) Annexin A5; and (b) a type III collagen. The fusion protein disclosed by the invention has the anti-inflammatory and anti-coagulation activity of annexin A5 and the repair promoting and anti-aging functions of III-type collagen, and has excellent stability, biocompatibility and application performance.