40 results about "Bacterial endotoxin" patented technology

This invention relates to the field of glycerol purification technology, specifically to a multi-stage distillation purification method for pharmaceutical-grade glycerol. Using industrially synthesized crude glycerol as raw material, the pretreated material is obtained through heating and degassing. Then, a first-stage molecular distillation removes low-boiling-point impurities, yielding an intermediate product. A second-stage molecular distillation further refines the product, collecting the gaseous glycerol fraction. Finally, under nitrogen protection, trace colored substances and residual polar impurities are removed through an adsorbent bed. After cooling, pharmaceutical-grade glycerol is obtained. Finally, the product is filtered and aseptically filled. This invention effectively removes aldehydes and ketones, residual solvents, and trace impurities from synthetic glycerol through the coupling of two-stage molecular distillation and adsorption processes. The resulting product has a purity ≥99.7%, total aldehyde and ketone impurities ≤0.005%, residual solvent ≤0.001%, bacterial endotoxin <2.5 EU / g, and batch-to-batch RSD <1%, meeting the standards for injection and suitable as an excipient in high-end pharmaceutical preparations.

This invention provides a drug for antagonizing bacterial endotoxins, its preparation method, and its application, belonging to the biomedical field. The composition consists of 25-55 parts of dried powdered traditional Chinese medicine extract, 25-70 parts of mixed essential oil powder, and 40-90 parts of lentinan powder. The traditional Chinese medicine extract is prepared by decoction of Scutellaria baicalensis, Lonicera japonica, Forsythia suspensa, and Isatis indigotica under reduced pressure at low temperature and spray drying. The mixed essential oil powder is prepared by encapsulating peppermint oil and eucalyptus oil with gelatin and hydroxypropyl cellulose and then spray drying under high pressure. This invention, through specific formulation and processing, significantly enhances the synergistic antagonistic effect of each component against endotoxins. Experiments show that this composition exhibits antagonistic activity far superior to ibuprofen in vitro; in vivo, it significantly reduces the endotoxin content in the plasma of 817 broiler chickens, rapidly alleviates LPS-induced fever and diarrhea, and improves decreased growth performance. Therefore, this invention is of great significance for reducing antibiotic dependence and improving the efficiency of livestock and poultry farming.

The invention discloses a bacterial endotoxin detection method of lipid nanoparticles and a demulsifier, and the detection method comprises the following steps: firstly preparing the demulsifier comprising polyoxyethylene lauryl ether, dimethyl sulfoxide and ethanol, then demulsifying a sample containing the lipid nanoparticles by using the demulsifier, and finally diluting the demulsified sample by using a diluent to obtain the bacterial endotoxin detection product. And determining the content of bacterial endotoxin. According to the invention, the solution containing polyoxyethylene lauryl ether, dimethyl sulfoxide and ethanol is used as the demulsifier of the lipid nanoparticles, the demulsification effect is good, the intracellular toxin wrapped in the demulsifier can be fully released, and the demulsifier does not influence the detection of the intracellular toxin; after the diluent is diluted, when the toxin in the cells is determined, the determination result meets the methodological requirements, and the accuracy of the detection result is ensured; in addition, the method provided by the invention is suitable for detecting different types of lipid nanoparticles.

The invention discloses a bacterial endotoxin detection analyzer, and particularly relates to the technical field of biological detection, and the bacterial endotoxin detection analyzer comprises a detection instrument, an MCU microcontroller, a color development detection module, a position sensing module, a motor driving module, an automatic temperature control module, a data transmission and processing system and upper computer software. The MCU coordinates the operation of each module, the color development detection module is combined with an electrochemical impedance spectroscopy and an aptamer biosensor to quantify the endotoxin concentration, and the other modules are respectively responsible for sample frame positioning, sample positioning, temperature control, data interaction, human-computer interaction and the like. The technical effects and advantages are remarkable: various stable voltages can be efficiently converted, and the system stability is improved; high-sensitivity and high-specificity signal acquisition and concentration quantification are realized, and the detection accuracy is enhanced; signal jitter is eliminated, motor out-of-step stalling is prevented, and reliability is improved; the temperature fluctuation is maintained between-0.2 DEG C and + 0.2 DEG C, so that the biochemical reaction is stable and repeatable.

The invention discloses a preparation method and a detection method of a detection liquid for determining bacterial endotoxin in letemovir injection, which can accurately detect the original endotoxin in the letemovir injection by effectively destroying an inclusion body of the letemovir injection. A simple, reliable and accurate method is established for detecting the endotoxin in the letemovir injection, and strict quality control on the letemovir injection is facilitated.

The invention discloses an application of an unmarked fluorescent aptamer sensor in endotoxin detection, according to an unmarked aptamer probe, a guanine-rich nucleic acid sequence is introduced to two ends of an endotoxin specific aptamer, the unmarked aptamer probe is combined with thioflavin T (ThT) to present a fluorescence'open 'state, when the endotoxin exists, the fluorescence is weakened, and when the endotoxin exists, the guanine-rich nucleic acid sequence is introduced to the two ends of the endotoxin specific aptamer, the guanine-rich nucleic acid sequence is introduced to the two ends of the thioflavin T (ThT); a linear relation exists between the fluorescence intensity change and the endotoxin content, and the method can be used for quantitative detection of endotoxin. Under the optimal condition, the linear range of the detector is 10-80 ng / mL, the limit of detection (LOD) is 3.26 ng / mL, the recovery rate in drinking water and fruit juice standard samples reaches 93.34-113.29%, and the relative deviation is 1.37-6.44%. The constructed non-labeled fluorescent aptamer sensor has the characteristics of strong specificity, high sensitivity and the like, and provides a promising solution for detecting endotoxin in a food sample.

The invention belongs to the technical field of medicine quality detection, and particularly relates to a method for detecting bacterial endotoxin of a raw material medicine in bromhexine hydrochloride injection. According to the method, a mode of heating and dissolving the hydrochloric acid solution with the pH value of 4.0 is adopted, and the 001 * 7 type cation exchange resin microcolumn purification is combined, so that the inhibition effect of cation impurities in the raw material medicine on agglutination reaction of the tachypleus amebocyte lysate can be effectively eliminated, and a stable and uniform test solution is prepared. According to the process, the minimum non-interference dilution multiple is obviously reduced to 160 times, and the operation error and the detection sensitivity loss caused by high-multiple dilution are reduced. As the dilution ratio is greatly reduced, a tachypleus amebocyte lysate with the sensitivity of 0.25 EU.mL <-1 > can be selected in the method. Compared with a high-sensitivity reagent needed in the prior art, the reagent of the specification is easier to purchase and lower in cost, solves the problems that the high-sensitivity reagent is large in daily purchase difficulty and high in price, and is more suitable for conventional application in industrial quality control.

The application discloses a compound amino acid injection (15-HBC) composition and a preparation method thereof, and belongs to the technical field of medicines. The compound amino acid injection (15-HBC) composition provided by the application comprises the following components in an amount of 250mL of the composition: isoleucine 1.532-2.298g, leucine 2.756-4.134g, lysine acetate 1.160-1.740g, methionine 0.500-0.750g, phenylalanine 0.640-0.960g, threonine 0.400-0.600g, tryptophan 0.180-0.270g, valine 1.772-2.658g, alanine 0.800-1.200g, arginine 1.160-1.740g, histidine 0.320-0.480g, proline 1.260-1.890g, serine 0.660-0.990g, glycine 0.660-0.990g, cysteine hydrochloride 0.040-0.060g, and appropriate amount of glacial acetic acid for pH adjustment, and the rest is injection water. The application also provides a preparation method of the compound amino acid injection (15-HBC) without sulfite antioxidant. Meanwhile, the activated carbon adsorption process is removed in the preparation, the control of raw material packages, production processes and the like is strengthened to control bacterial endotoxins, the production process is simplified, and the product quality is ensured.

This invention discloses a sample pretreatment method for detecting bacterial endotoxins in LNP-type mRNA vaccines. The method involves unpacking the sample with a 3-10% (v / v) Triton X-100 solution, followed by a 0.9% sodium chloride solution as the diluent in the sample gradient dilution step. This effectively eliminates interference from the positive control group in the gel limit method for detecting bacterial endotoxins in mRNA vaccines. The method provides accurate and reliable results and is suitable for the quality control of bacterial endotoxins in LNP-type mRNA vaccines.

Verification plates for a bacterial endotoxin reader are provided, namely a temperature verification plate (TVP) and optical verification plate (OVP). The TVP has a body configured to be placed on a spindle of said reader and rotated by said spindle. The body has a temperature verification circuit with a temperature sensor and a temperature indicator. The temperature sensor is configured to measure a temperature of the body rotated by the spindle of the reader. The temperature indicator optically represents a value of the temperature measured by the temperature sensor. The temperature indicator is readable by an optical bench of the reader. The OVP has a body with a plurality of apertures located along a periphery that line up with an optical bench of the reader. Light produced by a light source of the reader can pass through the aperture and an intensity measured by a photodetector of the reader.

The invention relates to the technical field of biological detection, in particular to a bacterial endotoxin detection method for a disposable liquid storage bag. The bacterial endotoxin test method of the disposable liquid storage bag comprises the following steps: filling water into the disposable liquid storage bag, and then carrying out constant-temperature shaking table treatment to prepare a test solution; performing an interference preliminary screening test on the test solution to determine the minimum non-interference dilution multiple of the test solution; and diluting the test solution by selecting the minimum non-interference dilution multiple determined by the interference preliminary screening test, and then detecting the bacterial endotoxin by adopting a dynamic color development method. According to the bacterial endotoxin detection method for the disposable liquid storage bag, the consumption of adopted key reagents is low, and the cost is remarkably reduced; a matched full-automatic system can detect as many as 19 samples at a time, the detection period is greatly shortened, and the error rate of manual operation is reduced.

To provide an ophthalmic composition of bevacizumab.SOLUTION: Provided is an ophthalmic composition of bevacizumab; a. herein, the limit of Bacterial Endotoxins Test (BET) is within the range of 0.001 to 0.4EU / mg, preferably within the range of 0.001 to 0.2Eu / mg, and more preferably within the range of 0.001 to 0.16Eu / mg; b. herein, a particulate substance is as follows; 1 to 50 particles≥diameter 10 μm per iv.ml, 0 to 5 particles≥diameter 25 μm per v.ml, and 0 to 2 particles≥diameter 50 μm per vi.ml; and c. herein, the composition has an aggregate of 0.1 to 5%, more preferably 0.1 to 4%, and most preferably 0.1 to 3.5% for 2 to 3 years, preferably for 2 to 2.5 years, and most preferably for 2 years.SELECTED DRAWING: None

The invention discloses a recombinant tachypleus amebocyte lyophilized preparation for bacterial endotoxin detection as well as a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is obtained by lyophilizing a lyophilized solution, wherein the lyophilized solution comprises the following components: mannitol, cane sugar, polyvinylpyrrolidone, recombinant human albumin, ascorbic acid, sodium chloride, inositol, L-arginine hydrochloride, ethylene glycol diethyl ether diamine tetraacetic acid, Tween 80, Proclin 300, Tris-HCl with the pH value of 7.0-8.0, a recombinant tachypleus amebocyte lyophilized preparation, a chromogenic substrate and a reaction buffer solution. The invention further discloses a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is prepared by matching a multi-element synergistic protection system with a seven-stage gradient temperature control freeze-drying process, and can be used for high-sensitivity and high-stability detection of bacterial endotoxin in drugs, medical instruments, biological products and clinical samples.

The utility model discloses a bacteria endotoxin gel method detection device based on automatic control, including work table, the upper end of work table is provided with sample holder, the right -hand member of sample holder is provided with dilution frame correspondingly, dilution frame is provided with three groups, the right -hand member of dilution frame is provided with oscillation frame correspondingly, the rear end of oscillation frame is installed tip head area correspondingly, this patent device easy operation, man -machine interface is friendly, has reduced manpower cost, has improved work efficiency, the device degree of automation is high, has reduced the risk factor that human interference possibly brings maximum limit, and whole device can realize automatic gel result determination simultaneously, result calculation can, and can real -time monitoring whole test process, realizes the traceability of metadata simultaneously, can carry out endotoxin gel method detection to a large number of samples simultaneously, adapts to the demand of large -scale detection.

1. The name of the design product: electronic device for IGL-800 automatic bacterial endotoxin detection system graphical user interface. 2. The use of the design product: for display and interaction. 3. The design points of the design product: in the graphical user interface. 4. The picture or photo that best indicates the design points: change state figure 1. 5. The use of the graphical user interface: for IGL-800 automatic bacterial endotoxin detection system. 6. The change state description of the graphical user interface: the IGL-800 automatic bacterial endotoxin detection system enters the interface as shown in the front view; input account password, click 【login】 button in the main view to enter change state figure 1; click 【experimental operation】 button in change state figure 1 to enter change state figure 2; click 【connection】 button in change state figure 2 to enter change state figure 3; after the operation in change state figure 3 is completed, enter change state figure 4; click 【sample entry】 button in change state figure 4 to enter change state figure 5; after the operation in change state figure 5 is completed, enter change state figure 6; click 【self-check complete】 button in change state figure 6 to enter change state figure 7; click 【continue】 button in change state figure 7 to enter change state figure 8; click 【add complete】 button in change state figure 8 to enter change state figure 9; click 【determine】 button in change state figure 9 to enter change state figure 10; click 【add complete】 button in change state figure 10 to enter change state figure 11; click 【determine】 button in change state figure 11 to enter change state figure 12; click 【new suction head box】 button in change state figure 12 to enter change state figure 13; click 【reaction curve】 button in change state figure 13 to enter change state figure 14.

The invention discloses a bacterial endotoxin detection method for cross-linked sodium hyaluronate gel containing poly-L-lactic acid microspheres. The method comprises the following steps: dissolving poly-L-lactic acid microspheres in gel by using trichloromethane as a weak polar solvent so as to release bacterial endotoxin wrapped by the L-lactic acid microspheres, extracting by using bacterial endotoxin inspection water, and inspecting the bacterial endotoxin. According to the method, through the pretreatment step of solvent dissolution-water phase extraction, the problem of bacterial endotoxin detection of implantable medical devices containing water-insoluble biopolymer materials and water-insoluble polymer materials is effectively solved, operation is easy and convenient, the result is reliable, and the method is suitable for quality control of related products in the medical device industry.

The present application provides a kind of detection method of bacterial endotoxin in PHA gel filling agent.The filling agent of the present application comprises PHA microspheres, gel carrier sodium carboxymethyl cellulose, osmotic pressure regulator, pH regulator and water for injection, the present application systematically studies the influence of gel carrier and osmotic pressure regulator on the performance of composition shear viscosity and osmotic pressure, and scientifically selects the excellent and stable gel prescription.The present application accidentally finds that the combination of water and a small amount of dichloromethane can realize the sufficient release of endotoxin, and accurately detect its content.The method of the present application can effectively ensure the safety of human medicine, and has important industrial value.

The application provides a fast-acting flunixin meglumine injection and a preparation method thereof, and belongs to the technical field of veterinary medicine, and contains the following components: flunixin meglumine 5-10 (w / v) %, alpha-pyrrolidone 10-30 (w / v) %, hydrophilic solvent 0.5-1 (w / v) %, antioxidant 0.1-0.3 (w / v) % and the balance of water for injection. The application takes flunixin meglumine as the main drug, matches alpha-pyrrolidone as a solvent and a targeting guide distribution component, uses a hydrophilic solvent to assist dissolution and promote absorption, and uses an antioxidant to inhibit the oxidative degradation of the main drug, so that the dissolution and absorption rate of the main drug and the target tissue distribution efficiency can be effectively improved, the storage stability can be ensured, the bacterial endotoxin and the injection site redness rate meet the requirements, the acute inflammation and pain of livestock and poultry can be quickly and efficiently relieved, and the demand for instant intervention in large-scale breeding can be met.

The utility model relates to biological medicine technical field especially, and it is degerming treatment device, including the reaction kettle of fixed installation on the ground, raw and auxiliary material filter cylinder and reaction liquid post filter cylinder, the export of raw and auxiliary material filter cylinder is connected to the entrance of reaction kettle through pipeline and delivery pump, and the export of reaction kettle is connected to the entrance of reaction liquid post filter cylinder through pipeline and delivery pump, the inner wall of raw and auxiliary material filter cylinder is fixedly connected with the placement strip, and the filter membrane is installed on the placement strip, and the flow guide assembly that can carry out the compression firm of filter membrane is provided on the filter membrane, and the same filter membrane and flow guide assembly of raw and auxiliary material filter cylinder are provided in reaction liquid post filter cylinder. The utility model adds 0.45 microns degerming filtration step before raw and auxiliary material feeding, sets up 0.22 microns degerming grade filter in the most critical position before spray drying, can filter the vast majority of bacteria cadaver and microparticle that possibly exist in polymerization reaction liquid, directly and efficiently reduces bacterial endotoxin content.

The present application relates to the technical field of bacterial endotoxin detection, in particular to a detection method of bacterial endotoxin of paliperidone palmitate. The detection method comprises: mixing paliperidone palmitate to be tested, a solvent and bacterial endotoxin test water to form a test solution, and then performing detection, wherein the solvent is a mixed solution formed by mixing tetrahydrofuran and Tween. The detection method provided by the present application can completely dissolve the paliperidone palmitate to be tested, the selected solvent does not interfere with the detection of bacterial endotoxin, effectively solves the problem that endotoxin is wrapped by the sample and cannot be completely released, and has very important significance for the safety of poorly soluble injections.

This invention provides a rapid detection kit for bacterial endotoxins in recombinant hepatitis B vaccine and its usage method. The kit includes the following items: one working standard for bacterial endotoxins, 10 Limulus amebocyte lysate (LAL) reagents, one 5ml vial of 1×PBS buffer solution (Solution A), one 50ml vial of water for bacterial endotoxin testing (BET) solution (Solution B), six pyrogen-free glass test tubes, two test tube racks, one ampoule opener, two sealing films, and one instruction manual. This kit is designed for the bacterial endotoxin test of recombinant hepatitis B vaccine (CHO cells). The solution concentration is pre-set according to the limits specified in the pharmacopoeia, eliminating the need for testing personnel to recalculate the dilution steps and solution concentrations according to the limits. The test can be completed by simply adding samples according to the instructions. The test is easy to repeat and suitable for personnel with or without experience in endotoxin testing.

The invention provides baicalin for an injection and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations, the preparation method comprises the following steps: taking a radix scutellariae medicinal material, extracting, adding low-carbon alcohol into the obtained solution, carrying out ultrafiltration, heating the obtained liquid medicine, carrying out heat preservation and acid adjustment, and sequentially carrying out heat preservation, filtering while hot, washing with hot low-carbon alcohol and drying to obtain the baicalin for the injection. And filtering to obtain the baicalin for the injection. The preparation method disclosed by the invention is few in steps, high in yield, low in cost and convenient for large-scale production; the baicalin produced by the method is high in purity, good in solubility, few in insoluble impurities and low in bacterial endotoxin, can meet the use requirements of injections, and can be used by the injections.

This application relates to novel pharmaceutical uses of rilpivirine, specifically its application in the preparation of drugs for the prevention and / or treatment of macrophage inflammatory diseases, sepsis, and sepsis-induced acute lung injury. Experiments have demonstrated that rilpivirine can significantly reduce gene expression of inflammatory factors in macrophage inflammation models, exhibits no significant toxicity at effective doses, and has high safety. Furthermore, rilpivirine can significantly alleviate acute lung injury induced by sepsis or lung exposure to bacterial endotoxins by inhibiting the expression of inflammatory factors, thereby improving the effect of acute lung injury in mice and significantly increasing the survival rate of mice with acute lung injury models.

The invention discloses a method for detecting bacterial endotoxin of degradable polyester, and relates to the technical field of insoluble substance impurity detection.The method comprises the steps that the degradable polyester is dissolved with dichloromethane, and an organic phase solution is obtained; bET water is added, and water-phase extract liquor is extracted through oscillation; and carrying out bacterial endotoxin detection on the test solution by using the tachypleus amebocyte lysate. According to the method, the degradable polyester is dissolved by adopting dichloromethane, so that the problem that the material is difficult to dissolve is solved, the interference effect on bacterial endotoxin detection is eliminated, the detection result is accurate and reliable, the reproducibility is good, and the method has important significance in guaranteeing the safety of the polymer medical material; more powerful technical support can be provided for quality control of related medical implant materials, and the safety and reliability in medical application are further improved.

Verification plates for a bacterial endotoxin reader are provided, namely a temperature verification plate (TVP) and optical verification plate (OVP). The TVP has a body configured to be placed on a spindle of said reader and rotated by said spindle. The body has a temperature verification circuit with a temperature sensor and a temperature indicator. The temperature sensor is configured to measure a temperature of the body rotated by the spindle of the reader. The temperature indicator optically represents a value of the temperature measured by the temperature sensor. The temperature indicator is readable by an optical bench of the reader. The OVP has a body with a plurality of apertures located along a periphery that line up with an optical bench of the reader. Light produced by a light source of the reader can pass through the aperture and an intensity measured by a photodetector of the reader.

This invention discloses a probiotic delivery system based on a core-shell hydrogel structure and its application, belonging to the field of biomedical technology. The system comprises a hydrogel capsule shell and a core containing probiotics. The hydrogel capsule shell isolates the probiotics from the external environment and adsorbs lipopolysaccharides produced by the probiotics. The internal pore size of the capsule shell is 200-300 nm, allowing external nutrients to enter and the release of therapeutically active metabolites secreted by the probiotics. The core is a sodium alginate hydrogel that effectively maintains bacterial activity and contains certain nutrients required by the bacteria. This probiotic delivery system provides dual isolation effects, combining physical and chemical isolation, avoiding the negative effects of bacterial endotoxins on the human body. It achieves a balance between bacterial isolation and drug penetration, exhibiting good long-term stability.

The application relates to the technical field of biological medicine, in particular to a bacterial endotoxin removing device. The removing column comprises a column body and a shaking mechanism; the inside of the column body is provided with a cavity, a plurality of activated carbon particles wrapped with cellulose acetate membranes are filled in the cavity, the two ends of the column body are respectively provided with openings, and a plurality of 0.22-micron microporous filter membranes are arranged at the ends of the column body at intervals; the shaking mechanism clamps and fixes the column body and is used for shaking the column body, so that the solution entering the inside of the column body fully contacts the activated carbon particles. The removing column can remove bacterial endotoxins in various product solutions, does not use stimulating solvents, does not damage the components in the product solutions, is simple to operate, is widely applicable, and is high in practicality.

The invention belongs to the field of bacterial endotoxin detection, and particularly relates to a method for detecting bacterial endotoxin in lornoxicam bulk drugs. The invention develops a method for detecting bacterial endotoxin in lornoxicam bulk drug, which is convenient to operate, and systematically and completely researches the applicability of the novel method. A basis is provided for formulating bacterial endotoxin inspection items in lornoxicam raw material quality standards, and a reference is provided for bacterial endotoxin inspection of other water-insoluble products at the same time. Compared with an original quality standard method, the method has the advantages that the operation steps are simplified, the recovery rate of endotoxin is increased, and the false negative rate and the detection limit of a detection result are remarkably reduced.

1. The name of the design product: electronic device for MB-80X bacterial endotoxin test system graphical user interface. 2. The use of the design product: for display and interaction. 3. The design points of the design product: in the graphical user interface. 4. The picture or photo that best indicates the design points: change state figure 1. 5. The use of the graphical user interface: for MB-80X bacterial endotoxin test system. 6. The change state description of the graphical user interface: the MB-80X bacterial endotoxin test system enters the interface as shown in the main view; enter the username and password in the main view, click the 【login】 button to enter change state figure 1; click the 【test collection】 button in change state figure 1 to enter change state figure 2; click the 【self-check】 button in change state figure 2 to enter change state figure 3; click the 【optical density】 button in change state figure 3 to enter change state figure 4; click the 【sample entry】 button in change state figure 4 to enter change state figure 5; click the 【complete】 button in change state figure 5 to enter change state figure 6; click the 【detect】 button in change state figure 6 to enter change state figure 7; click the 【reaction curve】 button in change state figure 7 to enter change state figure 8; after detection is completed, enter change state figure 9.

The utility model discloses an endotoxin detection kit, which relates to the field of biological detection kits, and comprises a detection kit, the top of the inner side of the detection kit is provided with a support frame, the top end of the support frame is uniformly provided with a plurality of clamping holes, and the inner side of each clamping hole is provided with a taking and sending assembly matched with the detection kit. Protective top covers are arranged at the top ends of the taking and sending assemblies; a kit cover matched with the supporting frame is arranged on one side of the top end of the detection kit. Under the matching action of the detection kit, the supporting frame, the clamping hole and the taking and conveying assembly, bacterial endotoxin in a biological product can be effectively detected and quantified, the quality and safety of the product are ensured, serious adverse reactions caused by the endotoxin are prevented, and the health of a patient is guaranteed; and meanwhile, the detection efficiency can be improved, the standardization degree of a laboratory working process is improved, and the method has important significance on pharmaceutical, medical and biological product industries.