74 results about "Bacterial endotoxin" patented technology

The invention discloses humanized III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. According to the method, firstly, on the basis of an original humanized III-type collagen gene, cell adhesion activity is taken as an evaluation index, a better cell adhesion sequence is screened out and spliced and integrated with the original collagen gene, and brand-new humanized III-type collagen is obtained. And constructing a recombinant expression vector of the humanized III type collagen containing the adhesion sequence by taking the probiotic EcN as a host cell, and introducing the recombinant expression vector into the EcN to obtain a strain of functional recombinant probiotic. Compared with a traditional escherichia coli engineering bacterium BL21 (DE3), the recombinant probiotics have good cell adhesion activity while efficiently expressing the humanized type III collagen, the bacterial endotoxin content of the recombinant humanized type III collagen obtained through purification meets the industrial standard, and the recombinant probiotics have good application prospects. The purification cost caused by industrial removal of endotoxin can be avoided, so that the production cost is reduced, and the method has a good application prospect.

The invention relates to an adjusting system applied to continuous kidney replacement treatment of acute kidney injury patients. Comprising the following steps: capturing the blood flow velocity and vascular endothelial permeability of glomerular capillaries in real time by using a micro sensor or a nano-scale optical imaging technology; the method comprises the following steps: pre-judging a risk time point of insufficient tissue perfusion through dynamic modeling of a capillary structure and a functional relationship; combining the microcirculation data with conventional biochemical indexes of the patient to create an early intervention model; when it is monitored that microcirculation perfusion is greatly reduced or kidney oxygen supply is insufficient, continuous renal replacement therapy CRRT parameter fine adjustment is triggered in advance; the method comprises the following steps: collecting plasma toxins of different patients at clinical and molecular levels, and constructing a common toxin map of the acute kidney injury AKI; whether the specific toxin concentration exceeds the standard or not is judged by combining a real-time sensor, and the removal priority is output in real time; a membrane material with an adjustable aperture or a special coating is adopted, so that inflammatory mediators and bacterial endotoxin molecules are adsorbed or intercepted, and the permeability of micromolecular electrolyte is kept.

The present invention discloses a sea dragon-derived antimicrobial peptide, SsPle, for antimicrobial applications in the complex environments of tropical islands and reefs. The amino acid sequence of this antimicrobial peptide is shown in SEQ ID NO. 3. This antimicrobial peptide, SsPle, exhibits good water solubility, broad antimicrobial activity, and highly stable antimicrobial properties under high temperature, high salt, and ultraviolet irradiation conditions. It can inhibit the release of free bacterial endotoxins and has significant application prospects as an eco-friendly antimicrobial agent in the unique environments of South China Sea islands and reefs.

The invention relates to low-fat collagen as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out acidolysis and enzymolysis on a pre-treated product; preheating, centrifuging and salting out the enzymolysis product; after salting out, carrying out secondary centrifugation, taking precipitate, re-dissolving the precipitate in an acetic acid solution, carrying out first pasteurization, and filtering; and carrying out ultrafiltration concentration on the filtered product, and carrying out secondary pasteurization on the concentrated solution to obtain the recombinant collagen. The collagen obtained by the extraction process disclosed by the invention is lower in grease content and lower in bacterial endotoxin content, and the purity, the safety, the thermal stability, the structural integrity, the applicability and the like of the collagen are guaranteed.

The invention belongs to the technical field of bacterial endotoxin detection, and particularly discloses a sulpiride bacterial endotoxin detection method which comprises the following steps: sequentially preparing a series of standard substance solutions, a test substance solution, a test substance positive control solution and an interference test solution; adding the series of standard substance solutions into tachypleus amebocyte lysate, detecting by adopting a dynamic nephelometry, and drawing a standard curve; respectively adding the test sample solution, the test sample positive control solution and the interference test solution into a limulus reagent, detecting by adopting a dynamic nephelometry, and calculating the bacterial endotoxin content in the sulpiride sample according to a standard curve linear regression equation. Compared with the prior art, the sulpiride sample is dissolved by using thionyl chloride, and then the sulpiride sample is further diluted by using the bacterial endotoxin inspection water to prepare the test solution, so that the problems that sulpiride is difficult to dissolve in the bacterial endotoxin inspection water and cannot effectively release bacterial endotoxin can be solved.

The invention discloses a medicinal material capable of being used in an injection as well as a preparation method and application of the medicinal material, and belongs to the technical field of medicines, and the main component of the medicinal material capable of being used in the injection comprises epsilon-polylysine. The invention provides a preparation process of a medicinal material capable of being used in an injection, and the medicinal material has a good recovery rate; experimental studies on bacterial endotoxin, safety and the like are carried out, the self-made medicinal material capable of being used in the injection has good safety, and the blank that the medicinal material capable of being used in the injection is prepared from food-grade epsilon-polylysine is filled.

The invention discloses a probiotic delivery system based on core-shell structure hydrogel and application of the probiotic delivery system, and belongs to the technical field of biological medicines.The probiotic delivery system based on the core-shell structure hydrogel comprises a hydrogel capsule shell and an inner core containing probiotics, the hydrogel capsule shell isolates the probiotics from the outside, and the inner core contains the probiotics; the hydrogel capsule comprises a shell and a core, the shell is made of sodium alginate, lipopolysaccharide generated by probiotics is adsorbed, the internal aperture of the shell of the hydrogel capsule is 200-300 nm, external nutrient substances are allowed to enter, metabolic substances secreted by the probiotics and having a treatment effect are allowed to go out, and the core is sodium alginate hydrogel which can well keep bacterial activity and contains certain nutrient substances required by bacteria. The probiotic delivery system has double isolation effects of physical isolation and chemical isolation, avoids negative effects of bacterial endotoxin on a human body, can realize bacterial isolation and drug permeation balance, and is good in long-term stability.

This invention relates to the field of glycerol purification technology, specifically to a multi-stage distillation purification method for pharmaceutical-grade glycerol. Using industrially synthesized crude glycerol as raw material, the pretreated material is obtained through heating and degassing. Then, a first-stage molecular distillation removes low-boiling-point impurities, yielding an intermediate product. A second-stage molecular distillation further refines the product, collecting the gaseous glycerol fraction. Finally, under nitrogen protection, trace colored substances and residual polar impurities are removed through an adsorbent bed. After cooling, pharmaceutical-grade glycerol is obtained. Finally, the product is filtered and aseptically filled. This invention effectively removes aldehydes and ketones, residual solvents, and trace impurities from synthetic glycerol through the coupling of two-stage molecular distillation and adsorption processes. The resulting product has a purity ≥99.7%, total aldehyde and ketone impurities ≤0.005%, residual solvent ≤0.001%, bacterial endotoxin <2.5 EU / g, and batch-to-batch RSD <1%, meeting the standards for injection and suitable as an excipient in high-end pharmaceutical preparations.

This invention provides a drug for antagonizing bacterial endotoxins, its preparation method, and its application, belonging to the biomedical field. The composition consists of 25-55 parts of dried powdered traditional Chinese medicine extract, 25-70 parts of mixed essential oil powder, and 40-90 parts of lentinan powder. The traditional Chinese medicine extract is prepared by decoction of Scutellaria baicalensis, Lonicera japonica, Forsythia suspensa, and Isatis indigotica under reduced pressure at low temperature and spray drying. The mixed essential oil powder is prepared by encapsulating peppermint oil and eucalyptus oil with gelatin and hydroxypropyl cellulose and then spray drying under high pressure. This invention, through specific formulation and processing, significantly enhances the synergistic antagonistic effect of each component against endotoxins. Experiments show that this composition exhibits antagonistic activity far superior to ibuprofen in vitro; in vivo, it significantly reduces the endotoxin content in the plasma of 817 broiler chickens, rapidly alleviates LPS-induced fever and diarrhea, and improves decreased growth performance. Therefore, this invention is of great significance for reducing antibiotic dependence and improving the efficiency of livestock and poultry farming.

The invention discloses a bacterial endotoxin detection method of lipid nanoparticles and a demulsifier, and the detection method comprises the following steps: firstly preparing the demulsifier comprising polyoxyethylene lauryl ether, dimethyl sulfoxide and ethanol, then demulsifying a sample containing the lipid nanoparticles by using the demulsifier, and finally diluting the demulsified sample by using a diluent to obtain the bacterial endotoxin detection product. And determining the content of bacterial endotoxin. According to the invention, the solution containing polyoxyethylene lauryl ether, dimethyl sulfoxide and ethanol is used as the demulsifier of the lipid nanoparticles, the demulsification effect is good, the intracellular toxin wrapped in the demulsifier can be fully released, and the demulsifier does not influence the detection of the intracellular toxin; after the diluent is diluted, when the toxin in the cells is determined, the determination result meets the methodological requirements, and the accuracy of the detection result is ensured; in addition, the method provided by the invention is suitable for detecting different types of lipid nanoparticles.

The invention discloses a promethazine hydrochloride injection and a preparation method thereof, and relates to the technical field of pharmaceutical preparations. Every 1 mL of the promethazine hydrochloride injection is prepared from the following raw materials and auxiliary materials: 20 to 30 mg of promethazine hydrochloride, 0.3 to 0.8 mg of anhydrous sodium sulfite, 0.5 to 1.5 mg of sodium pyrosulfite, 0.02 to 0.08 mg of citrulline, 0.02 to 0.05 mg of gallic acid and the balance of water for injection. The stability of the promethazine hydrochloride injection can be improved, the impurity content can be reduced, and it can be guaranteed that bacterial endotoxin meets the production requirement in the long-term storage process.

The invention discloses a bacterial endotoxin detection analyzer, and particularly relates to the technical field of biological detection, and the bacterial endotoxin detection analyzer comprises a detection instrument, an MCU microcontroller, a color development detection module, a position sensing module, a motor driving module, an automatic temperature control module, a data transmission and processing system and upper computer software. The MCU coordinates the operation of each module, the color development detection module is combined with an electrochemical impedance spectroscopy and an aptamer biosensor to quantify the endotoxin concentration, and the other modules are respectively responsible for sample frame positioning, sample positioning, temperature control, data interaction, human-computer interaction and the like. The technical effects and advantages are remarkable: various stable voltages can be efficiently converted, and the system stability is improved; high-sensitivity and high-specificity signal acquisition and concentration quantification are realized, and the detection accuracy is enhanced; signal jitter is eliminated, motor out-of-step stalling is prevented, and reliability is improved; the temperature fluctuation is maintained between-0.2 DEG C and + 0.2 DEG C, so that the biochemical reaction is stable and repeatable.

The invention discloses a preparation method and a detection method of a detection liquid for determining bacterial endotoxin in letemovir injection, which can accurately detect the original endotoxin in the letemovir injection by effectively destroying an inclusion body of the letemovir injection. A simple, reliable and accurate method is established for detecting the endotoxin in the letemovir injection, and strict quality control on the letemovir injection is facilitated.

The utility model relates to the technical field of distilled water machines, and discloses an S-shaped multi-effect distilled water machine which comprises a plurality of distillation towers, each distillation tower is of a vertical tube type falling film evaporation structure and consists of an upper evaporator and a lower separator, and the plurality of distillation towers comprise a primary distillation tower and a plurality of secondary distillation towers; the input end of the condenser is connected with a water pump, and the water pump is used for inputting raw material water into the condenser. According to the utility model, the bottom of each distillation tower is provided with an independent discharge port for independent discharge, the discharge is not gathered to the last tower for discharge, the concentration of endotoxin in the product injection water is lower and lower along with the continuous removal of concentrated water of each distillation tower, and the centrifugal separation efficiency of the next tower is also higher and higher in sequence, so that the production efficiency of the product injection water is improved. The result is that the bacterial endotoxin content of the product injection water finally collected to the condenser is extremely low, so that the injection water with extremely high quality is obtained.

The invention discloses a sodium oleate bacterial endotoxin detection method, which comprises: S1, adding a sodium oleate sample to be detected into BET water, mixing, and placing in a water bath to dissolve; s2, after dissolving, adjusting the PH to be neutral by using hydrochloric acid to obtain a test sample solution with the initial concentration of 100-200mg / ml, and diluting the test sample solution by using a diluent to obtain a series of test sample solutions with dilution concentrations; the diluent is diluted by the steps of a magnesium ion buffer solution, a Tween-containing BET aqueous solution and BET water; s3, detecting the diluted sample solution to be tested; and S4, setting the positive control solution, the positive control group and the negative control group of the test sample, performing the same detection with the test sample solution, and performing comparison. The method can be used for quantitatively, efficiently and quickly detecting the bacterial endotoxin in the sodium oleate sample to be detected.

The invention relates to a bacterial endotoxin reader verification plate and a method of use. Verification plates, i.e., a temperature verification plate (TVP) and an optical verification plate (OVP), for a bacterial endotoxin reader are provided. The TVP has a body configured to be placed on and rotated by a spindle of the reader. The body has a temperature verification circuit having a temperature sensor and a temperature indicator. The temperature sensor is configured to measure a temperature of the body rotated by the main shaft of the reader. The temperature indicator optically represents a value of the temperature measured by the temperature sensor. The temperature indicator is readable through an optical table of the reader. The OVP has a body with a plurality of apertures positioned along a perimeter, the apertures being aligned with an optical stage of the reader. Light generated by a light source of the reader may pass through the aperture, and the intensity is measured by a photodetector of the reader.

The invention discloses an application of an unmarked fluorescent aptamer sensor in endotoxin detection, according to an unmarked aptamer probe, a guanine-rich nucleic acid sequence is introduced to two ends of an endotoxin specific aptamer, the unmarked aptamer probe is combined with thioflavin T (ThT) to present a fluorescence'open 'state, when the endotoxin exists, the fluorescence is weakened, and when the endotoxin exists, the guanine-rich nucleic acid sequence is introduced to the two ends of the endotoxin specific aptamer, the guanine-rich nucleic acid sequence is introduced to the two ends of the thioflavin T (ThT); a linear relation exists between the fluorescence intensity change and the endotoxin content, and the method can be used for quantitative detection of endotoxin. Under the optimal condition, the linear range of the detector is 10-80 ng / mL, the limit of detection (LOD) is 3.26 ng / mL, the recovery rate in drinking water and fruit juice standard samples reaches 93.34-113.29%, and the relative deviation is 1.37-6.44%. The constructed non-labeled fluorescent aptamer sensor has the characteristics of strong specificity, high sensitivity and the like, and provides a promising solution for detecting endotoxin in a food sample.

The invention belongs to the technical field of medicine quality detection, and particularly relates to a method for detecting bacterial endotoxin of a raw material medicine in bromhexine hydrochloride injection. According to the method, a mode of heating and dissolving the hydrochloric acid solution with the pH value of 4.0 is adopted, and the 001 * 7 type cation exchange resin microcolumn purification is combined, so that the inhibition effect of cation impurities in the raw material medicine on agglutination reaction of the tachypleus amebocyte lysate can be effectively eliminated, and a stable and uniform test solution is prepared. According to the process, the minimum non-interference dilution multiple is obviously reduced to 160 times, and the operation error and the detection sensitivity loss caused by high-multiple dilution are reduced. As the dilution ratio is greatly reduced, a tachypleus amebocyte lysate with the sensitivity of 0.25 EU.mL <-1 > can be selected in the method. Compared with a high-sensitivity reagent needed in the prior art, the reagent of the specification is easier to purchase and lower in cost, solves the problems that the high-sensitivity reagent is large in daily purchase difficulty and high in price, and is more suitable for conventional application in industrial quality control.

The application discloses a compound amino acid injection (15-HBC) composition and a preparation method thereof, and belongs to the technical field of medicines. The compound amino acid injection (15-HBC) composition provided by the application comprises the following components in an amount of 250mL of the composition: isoleucine 1.532-2.298g, leucine 2.756-4.134g, lysine acetate 1.160-1.740g, methionine 0.500-0.750g, phenylalanine 0.640-0.960g, threonine 0.400-0.600g, tryptophan 0.180-0.270g, valine 1.772-2.658g, alanine 0.800-1.200g, arginine 1.160-1.740g, histidine 0.320-0.480g, proline 1.260-1.890g, serine 0.660-0.990g, glycine 0.660-0.990g, cysteine hydrochloride 0.040-0.060g, and appropriate amount of glacial acetic acid for pH adjustment, and the rest is injection water. The application also provides a preparation method of the compound amino acid injection (15-HBC) without sulfite antioxidant. Meanwhile, the activated carbon adsorption process is removed in the preparation, the control of raw material packages, production processes and the like is strengthened to control bacterial endotoxins, the production process is simplified, and the product quality is ensured.

The present invention provides a method for detecting bacterial endotoxins in drugs containing a quinoline ring structure, and relates to the technical field of bacterial endotoxin detection. Specifically comprising the following steps: first dissolving a drug containing a quinoline ring structure with a solvent to obtain a test sample stock solution to release bacterial endotoxins in the drug; then diluting the stock solution to reduce the interference of the drug's own properties on bacterial endotoxins; and finally detecting bacterial endotoxins by dynamic colorimetry. Wherein, the solvent is N, N-dimethylformamide with a volume fraction of 60-70%; the drug containing a quinoline ring structure includes imiquimod; the test sample stock solution concentration is 0.1-10 mg / ml, and the stock solution dilution multiple is 10-1000 times. The technical solution provided by the present invention solves the problem that drugs containing a quinoline ring structure in the prior art are insoluble in water, and effectively improves the accuracy of detecting the bacterial endotoxin content of drugs containing a quinoline ring structure. Moreover, the conventionally adopted gel method is avoided, thereby solving the problem of false negative results of taking the supernatant and detecting it by gel method.

The utility model discloses a fixing device used in a bacterial endotoxin gel determination method, which comprises a test tube rack, the test tube rack comprises an upper plate and a lower plate, the outer surface of the upper plate is provided with placing holes, the outer surface of the lower plate is provided with flow guide holes, the upper surface of the upper plate is provided with a multifunctional test tube clamp, and the upper plate is provided with a clamping groove. The multifunctional test tube clamp comprises a test tube clamp body and an upper cover, the test tube clamp body comprises a frame body and a clamping mechanism, a rectangular groove is formed in the frame body, the clamping mechanism is located in the rectangular groove, the clamping mechanism comprises a connecting plate and a clamping plate, the side inner wall of the rectangular groove is fixedly connected with the connecting plate, and the clamping plate is located in the rectangular groove. The side surface of the connecting plate is fixedly connected with the clamping plates, the two clamping plates are symmetrically distributed, the outer side of a test tube can be rapidly clamped through the clamping mechanism, and the situation that a sample shakes to interfere with an experiment result in the process that the sample tube enters the constant-temperature water bath box is avoided.

This invention discloses a sample pretreatment method for detecting bacterial endotoxins in LNP-type mRNA vaccines. The method involves unpacking the sample with a 3-10% (v / v) Triton X-100 solution, followed by a 0.9% sodium chloride solution as the diluent in the sample gradient dilution step. This effectively eliminates interference from the positive control group in the gel limit method for detecting bacterial endotoxins in mRNA vaccines. The method provides accurate and reliable results and is suitable for the quality control of bacterial endotoxins in LNP-type mRNA vaccines.

Verification plates for a bacterial endotoxin reader are provided, namely a temperature verification plate (TVP) and optical verification plate (OVP). The TVP has a body configured to be placed on a spindle of said reader and rotated by said spindle. The body has a temperature verification circuit with a temperature sensor and a temperature indicator. The temperature sensor is configured to measure a temperature of the body rotated by the spindle of the reader. The temperature indicator optically represents a value of the temperature measured by the temperature sensor. The temperature indicator is readable by an optical bench of the reader. The OVP has a body with a plurality of apertures located along a periphery that line up with an optical bench of the reader. Light produced by a light source of the reader can pass through the aperture and an intensity measured by a photodetector of the reader.

The invention relates to the field of collagen preparation, in particular to a preparation method for improving the biological safety of collagen, a product and application. The preparation method comprises the following steps: pretreating raw materials; performing enzymolysis on the pre-treated product, and performing salting-out on the enzymolysis product; after redissolving the precipitate separated out by salting out, filtering by using a diatomite filter; and carrying out ultrafiltration and concentration on the filtered product to obtain the collagen. On the basis of ensuring the purity, the collagen obtained by the extraction process disclosed by the invention is higher in biological safety, sterile, lower in pathogen content and lower in bacterial endotoxin content, and can be ensured to be applied to the medical field.

The invention relates to the technical field of biological detection, in particular to a bacterial endotoxin detection method for a disposable liquid storage bag. The bacterial endotoxin test method of the disposable liquid storage bag comprises the following steps: filling water into the disposable liquid storage bag, and then carrying out constant-temperature shaking table treatment to prepare a test solution; performing an interference preliminary screening test on the test solution to determine the minimum non-interference dilution multiple of the test solution; and diluting the test solution by selecting the minimum non-interference dilution multiple determined by the interference preliminary screening test, and then detecting the bacterial endotoxin by adopting a dynamic color development method. According to the bacterial endotoxin detection method for the disposable liquid storage bag, the consumption of adopted key reagents is low, and the cost is remarkably reduced; a matched full-automatic system can detect as many as 19 samples at a time, the detection period is greatly shortened, and the error rate of manual operation is reduced.

To provide an ophthalmic composition of bevacizumab.SOLUTION: Provided is an ophthalmic composition of bevacizumab; a. herein, the limit of Bacterial Endotoxins Test (BET) is within the range of 0.001 to 0.4EU / mg, preferably within the range of 0.001 to 0.2Eu / mg, and more preferably within the range of 0.001 to 0.16Eu / mg; b. herein, a particulate substance is as follows; 1 to 50 particles≥diameter 10 μm per iv.ml, 0 to 5 particles≥diameter 25 μm per v.ml, and 0 to 2 particles≥diameter 50 μm per vi.ml; and c. herein, the composition has an aggregate of 0.1 to 5%, more preferably 0.1 to 4%, and most preferably 0.1 to 3.5% for 2 to 3 years, preferably for 2 to 2.5 years, and most preferably for 2 years.SELECTED DRAWING: None

The present invention provides the use of a pharmaceutical composition in the preparation of a medicament for treating diseases associated with high permeability of the blood-brain barrier and intestinal barrier. The pharmaceutical composition contains pharmaceutically acceptable β-hydroxybutyrate and vitamin D as active ingredients. In classical systemic inflammations of nerves, intestines, etc. induced by bacterial endotoxin lipopolysaccharide (LPS), the pharmaceutical composition of the present invention can well reduce the high permeability of the blood-brain barrier and intestinal barrier, and play a role in treating central nervous system inflammation, nerve damage, behavioral abnormalities, etc.

The invention discloses a recombinant tachypleus amebocyte lyophilized preparation for bacterial endotoxin detection as well as a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is obtained by lyophilizing a lyophilized solution, wherein the lyophilized solution comprises the following components: mannitol, cane sugar, polyvinylpyrrolidone, recombinant human albumin, ascorbic acid, sodium chloride, inositol, L-arginine hydrochloride, ethylene glycol diethyl ether diamine tetraacetic acid, Tween 80, Proclin 300, Tris-HCl with the pH value of 7.0-8.0, a recombinant tachypleus amebocyte lyophilized preparation, a chromogenic substrate and a reaction buffer solution. The invention further discloses a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is prepared by matching a multi-element synergistic protection system with a seven-stage gradient temperature control freeze-drying process, and can be used for high-sensitivity and high-stability detection of bacterial endotoxin in drugs, medical instruments, biological products and clinical samples.

Thermal disinfection equipment with a rapid cooling function relates to the technical field of thermal disinfection equipment, is connected between a water return pipeline and a pure water pipeline of hemodialysis water production equipment and a dialysis use point, and comprises an electric three-way valve A on the water return pipeline and an electric three-way valve B on the pure water pipeline, and a disinfection pipeline is connected between the electric three-way valve A and the electric three-way valve B; a hot water pump, a flow switch, an electromagnetic heating device and a temperature switch are sequentially arranged on the disinfection pipeline. An opposite flushing pipeline is connected between the disinfection pipeline close to one side of the electric three-way valve B and the pure water pipeline close to one side of the hemodialysis water production equipment, an electric ball valve A, a check valve, a stop valve and an electric ball valve B are arranged on the opposite flushing pipeline, and a drainage pipeline is connected to the opposite flushing pipeline between the electric ball valve A and the check valve. According to the thermal disinfection equipment, a traditional heating water tank is abandoned, bacteria and endotoxin breeding is inhibited, secondary pollution is eradicated, rapid cooling is achieved through valve switching and cold and hot water opposite flushing, and time is saved.

The utility model discloses a bacteria endotoxin gel method detection device based on automatic control, including work table, the upper end of work table is provided with sample holder, the right -hand member of sample holder is provided with dilution frame correspondingly, dilution frame is provided with three groups, the right -hand member of dilution frame is provided with oscillation frame correspondingly, the rear end of oscillation frame is installed tip head area correspondingly, this patent device easy operation, man -machine interface is friendly, has reduced manpower cost, has improved work efficiency, the device degree of automation is high, has reduced the risk factor that human interference possibly brings maximum limit, and whole device can realize automatic gel result determination simultaneously, result calculation can, and can real -time monitoring whole test process, realizes the traceability of metadata simultaneously, can carry out endotoxin gel method detection to a large number of samples simultaneously, adapts to the demand of large -scale detection.