Freeze-dried vaccine protective agent, freeze-dried varicella attenuated live vaccine and preparation methods of freeze-dried vaccine protective agent and freeze-dried varicella attenuated live vaccine

Abstract

The invention discloses a freeze-dried vaccine protective agent, a freeze-dried varicella attenuated live vaccine and preparation methods of the freeze-dried vaccine protective agent and the freeze-dried varicella attenuated live vaccine, belongs to the field of vaccine production and preparation processes, and solves the problem that an existing freeze-dried vaccine protective agent of a varicella vaccine contains gelatin, or contains dextranum and still contains human serum albumin even if the gelatin is removed, so that a potential hazard still exists in the safety of medication of the vaccine. The freeze-dried vaccine protective agent contains mycose, sodium glutamate, urea, L-arginine and a 199 culture medium, and does not contain macromolecular allergen ingredients such as gelatin, dextranum and human serum albumin; the bacterial endotoxin content is low; the varicella vaccine prepared by using the protective agent is low in endotoxin content and residual content of bovine serum albumin and antibiotics, good in stability, safe and effective; the main point is direct infection after passage; the cells are cleaned in the next day after infection, and are replaced with a serum-free maintenance fluid; the operation is simple; the cost is low; regular and uniform formation of cytopathy is facilitated; and the residual content of the bovine serum albumin can be greatly lowered.

Description

technical field [0001] The invention belongs to the technical field of vaccine production and preparation technology, and in particular relates to a vaccine freeze-drying protectant, a freeze-dried live attenuated varicella vaccine and a preparation method thereof. Background technique [0002] Varicella (chickenpox) is a common highly contagious disease in childhood, which is mainly transmitted through air droplets and contact. After recovery from the initial infection, latent infection is common, and reactivation of the latent virus can cause herpes zoster. The clinical features of chickenpox are rashes on the skin and mucous membranes of the whole body in batches, and maculopapular rashes, papules, herpes and scabs exist at the same time. 96% of the cases have obvious clinical symptoms, and only 4% are subclinical infections. In the temperate regions of the northern hemisphere, it is easy to The secondary infection rate of close family contact of infected persons is close...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the existing varicella vaccine lyoprotectant contains gelatin, or contains dextran although the gelatin has been removed, and some formulas still contain human albumin, so that there are still potential hidden dangers in the drug safety of the vaccine, the present invention Provided is a gelatin-free, dextran-free, and serum-albumin-free vaccine freeze-drying protectant, a freeze-dried live attenuated varicella vaccine prepared by using the protectant, and a preparation method thereof

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