The invention discloses a production method of an organic and inorganic composite fertilizer containing amino acid trace element chelates, and the product belongs to the field of composite fertilizer. The organic and inorganic composite fertilizer is composed of urea, monoammonium phosphate, potassium sulphate, a dry mixture of pig manure and cow manure, a mixture of peanut straw and rape stalk, a mixture of cotton seed cake and rapeseed cake, a mixture of rice husk and wheat bran, dry pollution-free river mud, plant ash, distillers' grain, biogas residue, an organic fertilizer leavening agent, a sterilizing and deodorizing agent, amino acid chelated calcium, amino acid chelated copper, amino acid chelated iron, amino acid chelated manganese, amino acid chelated zinc, and ammonium molybdate; the preparation method of the composite fertilizer comprises the following steps: preparing the raw materials mentioned above, and then subjecting the raw materials to processes of grinding, fermenting, mixing, granulating, and drying so as to obtain the composite fertilizer. The method has the advantages of simple technology, universal equipment, and high quality product. The product contains a large amount of inorganic substances, organic substances and trace elements, has comprehensive nutritional components and a long fertilizing effect, and can achieve the complementary advantages of organic substances and inorganic substances.