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Processing method of sea cucumber glycopeptides chelated calcium

A processing method, sea cucumber glycopeptide technology, applied in the field of aquatic product processing

Active Publication Date: 2016-03-02
烟台参福元海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium hydroxide is added in the process of its process, which has certain food safety hazards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Sea cucumber mouthparts are cleaned, steamed for 15 minutes, soaked in pure water at 4°C for 24 hours, added with pure water at a material-to-water ratio of 1:4 for beating, and homogenized. Using papain and compound protease, each added at the same time at a ratio of 1% (W / W) of the material, and enzymatically hydrolyzed at 60°C for 3 hours. Then use flavor protease accounting for 0.5% (W / W) of the material to continue enzymatic hydrolysis at 50°C for 1 hour to complete the enzymatic hydrolysis. After the enzymatic solution is continuously centrifuged, sea cucumber glycopeptide solution and sea cucumber calcium precipitate are obtained. After the sea cucumber glycopeptide enzymatic hydrolysis solution is intercepted and concentrated by the ultrafiltration membrane, the sea cucumber glycopeptide concentrate with a molecular weight cut-off greater than 30KDa, a polysaccharide concentration of 5mg / mL, and a purity of 90% is obtained; at the same time, the molecular weight...

Embodiment 2

[0030]Sea cucumber mouthparts are cleaned, steamed for 20 minutes, soaked in pure water at 4°C for 24 hours, then added with pure water at a material-to-water ratio of 1:4 for beating and homogenization. Add compound protease at a ratio of 0.5% (W / W) of the material first, and enzymolyze it at 50°C for 2 hours; then add papain at a ratio of 1% (W / W) of the material, and enzymatically digest it at 65°C Solution for 1 hour. Then, use alkaline protease accounting for 1% (W / W) of the material to continue enzymatic hydrolysis at 45°C for 1 hour to complete the enzymatic hydrolysis. After the enzymatic solution is continuously centrifuged, sea cucumber glycopeptide solution and sea cucumber calcium precipitate are obtained. After the sea cucumber glycopeptide enzymatic hydrolyzate is intercepted and concentrated by the ultrafiltration membrane, the sea cucumber glycopeptide concentrate with a molecular weight cut-off greater than 10KDa, a polysaccharide concentration of 10mg / mL, an...

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Abstract

The invention relates to a processing method of sea cucumber glycopeptides chelated calcium. The method comprises the following steps: A. material treatment: cleaning sea cucumber mouthparts, cooking, soaking, beating, and homogenizing; B. biological enzymatic hydrolysis: choosing a variety of protease for composite segmented enzymatic hydrolysis; C. centrifugal separation: conducting continuous centrifugal separation on an enzymolysis liquid to obtain the sea cucumber glycopeptides solution and sea cucumber calcium precipitate; D. filtration, concentration and grading: subjecting the enzyme liquid to ultrafiltration fractionation concentration, and desalinating small peptide filtered solution by nanofiltration; E. preparation of sea cucumber calcium by organic acidolysis; G. chelating reaction: promoting the chelating of sea cucumber calcium and glycopeptides of sea cucumber by microwave assistance; and H. after the reaction, carrying out centrifuging separation, and drying the precipitate and supernatant respectively. The present invention comprehensively utilizes the sea cucumber processing waste to prepare sea cucumber autologous calcium and autologous glycopeptides for chelation. The method on the one hand is beneficial to playing their biological effects, and on the other hand can also obtain five products with different specific biological functions.

Description

technical field [0001] The invention relates to the technical field of aquatic product processing, in particular to a processing method for sea cucumber glycopeptide chelated calcium. Background technique [0002] Calcium is one of the essential mineral elements for the human body, and it is also the element with the most content. It has many important physiological functions. Calcium ions are neurotransmitters that regulate muscle tension and are of great significance to bone health. At present, due to changes in lifestyle, calcium deficiency has gradually become a global health problem, especially in special populations such as the elderly, children, and pregnant women. Effective calcium supplementation is of great significance to improving people's physical fitness and quality of life. [0003] Sea cucumber is a traditional valuable nourishing product, and has the reputation of "Ginseng in the sea". Relevant studies have proved that sea cucumber is a high-protein, low-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00
CPCA23V2002/00A23V2200/324A23V2200/308A23V2200/306
Inventor 张健赵云苹井月欣刘昕王共明高继庆
Owner 烟台参福元海洋科技有限公司
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