387 results about "Alkaline protease" patented technology

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention discloses sturgeon roe peptide for preventing alcoholic liver injury as well as a preparation method and application of the sturgeon roe peptide. The peptide sequence is ILKPTDAGTL, ALPLDPK, LGGL, VNRDL, LLGQ, VFVLN or LAGL. The preparation method specifically comprises the following steps: (1) adding deionized water into the sturgeon roes, and homogenizing; (2) adjusting the pH value, adding alkaline protease, carrying out enzymolysis, carrying out enzyme inactivation, and cooling; and (3) performing centrifugal treatment, and taking supernate to obtain the traditional Chinese medicine composition. The sturgeon roe peptide disclosed by the invention has the efficacy of preventing or treating alcoholic liver injury, and is remarkable in hangover alleviating effect, simple to prepare and low in cost.

The invention relates to a preparation method of a placenta active factor as well as a product and application of the placenta active factor, and the preparation method comprises the following steps: soaking and rinsing a full-month normal delivery placenta, and homogenizing to obtain homogenate; repeatedly freezing and thawing the homogenate to collect a thaw solution, centrifuging the thaw solution, taking a supernatant, mixing the supernatant with a compound enzyme, and carrying out ultrasonic enzymolysis extraction to obtain an enzymolysis product; and centrifuging the enzymolysis product, taking the supernatant, and carrying out membrane filtration to obtain the placenta active factor. The compound enzyme comprises a combination of animal protein hydrolysis compound enzyme, alkaline protease, neutral protease and papain. The placenta tissue is extracted through cooperation of the specific compound enzyme and ultrasonic waves, the placenta tissue and the specific compound enzyme have a synergistic effect, the extraction efficiency can be further improved, the content of active ingredients in the extract is high, the biological activity is high, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention belongs to the technical field of bioengineering, and particularly relates to an alkaline protease mutant which is obtained through molecular modification and has improved enzyme activity and thermal stability and application of the alkaline protease mutant. An ALK wild type is mutated by utilizing a site-directed mutagenesis technology to obtain a mutant T56C / A90C of which the enzyme activity is improved relative to that of the wild type at 40 DEG C, and the mutant T56C / A90C is subjected to heat preservation at 60 DEG C for 4 hours to determine the protease activity and residual enzyme activity and has certain activity in a high-temperature environment, so that the heat stability of alkaline protease is improved, and the alkaline protease can play a role in a relatively high alkaline environment; therefore, the method can be widely applied to a plurality of industries such as washing, leather, medicine, feed, silk, food and the like.

The invention discloses a preparation method of sturgeon roe peptide freeze-dried powder with an anti-aging effect. The preparation method specifically comprises the following steps: (1) unfreezing sturgeon roes, adding water, uniformly stirring, grinding and crushing, and adjusting the pH value; (2) heating and naturally cooling; (3) adding alkaline protease, uniformly stirring, carrying out enzymolysis, and adding starch sodium octenylsuccinate and vitamin E powder; (4) carrying out enzyme inactivation, naturally cooling, and adjusting the pH value; (5) carrying out rough filtration and centrifugal treatment, taking supernate, adsorbing, filtering and taking filtrate; and (6) carrying out plate frame filtration, taking filtrate, carrying out vacuum freeze drying, screening and packaging, thereby obtaining the product. The preparation method disclosed by the invention has the advantages of simplicity and convenience in operation, low cost, high embedding yield and the like, and has important significance in improving the stability and biological activity of the sturgeon roe peptide. Moreover, the sturgeon roe peptide freeze-dried powder has an anti-aging effect, and can be widely applied to oral liquid, solid beverages, tablet candies and the like in common foods.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention belongs to the field of egg by-product processing, and particularly relates to a preparation method and application of eggshell membrane peptides, the preparation method of the eggshell membrane peptides comprises the following steps: S1, drying and crushing cleaned eggshells, and then separating eggshell membranes; s2, putting the eggshell membrane into a steam explosion machine, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.5 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; s3, adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-9 according to the solid-liquid ratio of 1: (20-30), then adding alkaline protease according to the enzyme addition amount of 5000-15000U / g, carrying out enzymolysis for 5-10 hours at the temperature of 35-65 DEG C, then inactivating for 3-5 minutes at the temperature of 100 DEG C, filtering enzymatic hydrolysate, taking filtrate, centrifuging, taking supernate, and freeze-drying to obtain polypeptide; s4, polypeptide obtained in S3 is dissolved in an activation solution to be activated for 8-10 h at the temperature of 4-8 DEG C; and S5, separating the sample activated in the step S4 through a Sephadex G-15 chromatographic column to obtain the activated eggshell membrane peptide. Based on the method, the preparation efficiency is high, and the prepared eggshell membrane peptide is high in antioxidant activity.

The present application relates to the technical field of oat bran deep processing, and particularly relates to an oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, and a preparation method and application thereof.The present application is prepared by adding alkaline protease and ficin for double-enzyme step-by-step hydrolysis after homogenate, enzymolysis and alkali extraction and acid precipitation pretreatment, and then filtering, ultrafiltration desalination and drying, so that the oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity is obtained.The present application is simple to operate, low in equipment requirement, and suitable for industrial large-scale production.The prepared oat active polypeptide has GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, has a significant inhibitory effect on pancreatic lipase and alpha-glucosidase, has an obvious GLP-1 secretion promotion effect, and has a significant blood sugar reduction and lipid reduction effect.

The invention discloses a preparation method of black garlic polysaccharide, which comprises the steps of extraction, enzymolysis deproteinization, ultrafiltration, concentration, drying, crushing and the like, and'enzymolysis deproteinization + ultrafiltration 'is the core step of the method. Wherein the enzymolysis deproteinization can be selected from papain, neutral protease, acid protease, alkaline protease and the like or a combination of the papain, the neutral protease, the acid protease and the alkaline protease. In the ultrafiltration step, an organic ultrafiltration membrane or an inorganic ceramic membrane or a combination of the organic ultrafiltration membrane and the inorganic ceramic membrane can be selected. The method has the remarkable advantages of simple process flow, low cost, safety, high efficiency, good finished product quality and the like. Pharmacological experiments prove that the black garlic polysaccharide prepared by the method disclosed by the invention has a remarkable anti-constipation effect, and can be used for preparing anti-constipation healthy products, including medicines, health-care foods and foods.

The invention relates to the technical field of active peptides, in particular to a myocardial active peptide capable of relieving heart failure and protecting heart and a preparation method thereof. The method takes pig hearts as raw materials, and sequentially comprises the following steps: pretreating the pig hearts, adding water, and homogenizing; adding alkaline protease to carry out first constant-temperature enzymolysis; adding neutral protease and flavourzyme to carry out second constant-temperature enzymolysis; then lipase is added for third-time constant-temperature enzymolysis, and enzyme deactivation and cooling are carried out; and finally, adding activated carbon into the enzymatic hydrolysate, filtering with diatomite, and spray-drying to obtain the myocardial active peptide. According to the method, the yield and the purity of the active peptide are remarkably improved through a synergistic process of three-step enzymolysis and activated carbon purification, the obtained product is rich in small molecule peptide with a definite heart protection function, and a new way is provided for high-value utilization of pig heart resources and development of functional products for preventing and treating heart failure.

The invention discloses mandulapalka milk and a preparation method thereof, and belongs to the technical field of beverage processing. According to the technical scheme provided by the invention, the preparation method of the degreased cyperus esculentus milk enzymolysis beverage comprises the following steps: adding high-temperature amylase into a degreased cyperus esculentus meal aqueous solution for enzymolysis for 1 hour, and then adding alkaline protease and flavourzyme for enzymolysis for 1 hour to obtain an enzymolysis solution; and finally, emulsifying residual starch and dextrin in the enzymatic hydrolysate by using soybean protein isolate and corn oil, and homogenizing by using a high-pressure homogenizer to obtain the cyperus esculentus beverage. According to the method, a multi-enzymolysis technology is adopted, the enzymolysis speed can be increased, the taste can be improved, a double-enzymolysis method is adopted, the enzymolysis time is shortened, starch is subjected to enzymolysis more sufficiently, and the flavor is improved. The defatted cyperus esculentus beverage prepared by the method disclosed by the invention is appropriate in sweetness, silky in mouth, mellow and strong in fragrance and unique in flavor, and is a healthy and mellow leisure beverage.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention provides an albumin peptide for promoting growth and development as well as a preparation method and application thereof, and belongs to the technical field of food science. According to the albumin peptide provided by the invention, denatured egg white is taken as a substrate, firstly, chymotrypsin is used for prehydrolysis, after prehydrolysis is completed, the temperature and pH are adjusted, and then alkaline protease and chymotrypsin are added for synergistic hydrolysis. The prepared albumin peptide is small in molecular weight and easy to digest and absorb, and has the effects of promoting growth and development, improving macrophage proliferation capacity, improving immunity and the like.

The invention provides collagen tripeptide as well as a preparation method and application thereof, and belongs to the technical field of active peptide. The invention provides a preparation method of collagen tripeptide, which comprises the following steps: carrying out enzymolysis treatment on tilapia scales and high-temperature-resistant protease, then carrying out enzymolysis treatment by using alkaline protease and neutral protease in sequence, filtering enzymatic hydrolysate by using a ceramic membrane and an organic filter membrane in sequence, and intercepting by using a 400Da nanofiltration membrane to obtain collagen tripeptide. The permeate contains active peptide mainly composed of collagen tripeptide. After the permeate is concentrated and dried, the collagen tripeptide powder is detected, and the protein content reaches 96.2%, the GPH content is 3.8%, and the CTP content is 35.09%. The collagen tripeptide powder has better DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity; the traditional Chinese medicine composition has an obvious ROS removing effect, a beta-galactosidase activity inhibiting effect and a telomerase activity enhancing promoting effect on a hydrogen peroxide induced zebrafish aging model, and shows an obvious anti-aging effect.

The invention belongs to the technical field of microorganisms and genetic engineering, and particularly relates to an alkaline protease mutant and application thereof in leather unhairing. According to the invention, alkaline protease derived from Virgibacillus byunsanensis and alkaline protease derived from Ordinitinibacillus caprae are subjected to directed evolution in vitro, so that an alkaline protease mutant of which the enzyme activity and the stability are remarkably improved is obtained, and active extracellular expression of a coding gene of the mutant is realized in an engineering bacterium bacillus subtilis WB600. The optimal reaction temperature of the alkaline protease mutant is 45 DEG C, the optimal pH value of the alkaline protease mutant is 8, the residual enzyme activity of the alkaline protease mutant can be kept at 90% or above after the alkaline protease mutant is incubated for 12 h under the conditions that the pH is 8 and the temperature is 50 DEG C or below or the temperature is 45 DEG C and the pH is 7-9, and the alkaline protease mutant has good thermal stability and pH stability, has high tolerance to NaCl, is applied to unhairing of salt wet sheepskin subjected to preservative treatment through NaCl with the concentration smaller than or equal to 100 g / L, and has good application prospects. Under the conditions that the pH is 7-9 and the temperature is 40-50 DEG C, the unhairing time is shortened to 1.5-2 hours.

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.

The invention provides application of sturgeon swimming bladder collagen peptide in preparation of a medicine for premature ovarian failure, and relates to the technical field of protein peptide. According to the method, the sturgeon swim bladder is subjected to resource utilization, so that the waste of the sturgeon swim bladder can be effectively reduced, the sturgeon resource can be sustainably utilized, the extraction rate of the sturgeon swim bladder collagen is relatively high, and industrial production is facilitated; meanwhile, collagen peptide obtained after enzymolysis treatment is conducted through alkaline protease can achieve the effects of preventing, treating, inhibiting and improving the premature ovarian failure.

The invention relates to the technical field of degreasing agents, in particular to a high-efficiency energy-saving degreasing agent and a preparation method thereof, and the high-efficiency energy-saving degreasing agent comprises the following components in parts by weight: 6-9 parts of recombinant thermostable lipase; 4-7 parts of alkaline protease; 1.5 to 2.5 parts of nano silver particles wrapped by chitosan; 0.8 to 1.8 parts of beta-cyclodextrin modified carbon quantum dots; 12 to 18 parts of sodium alginate based intelligent hydrogel microspheres; 18 to 22 parts of lauryl glucoside; 12 to 18 parts of coconut oil-based betaine; 6 to 9 parts of propylene glycol; 4 to 7 parts of d-limonene; 10 to 14 parts of polyethylene glycol 400; 2 to 4 parts of dipotassium phosphate; according to the modified recombinant thermostable lipase, the low-temperature activity and the pH adaptability are remarkably improved while the high catalytic efficiency is maintained, and the antibacterial performance is enhanced by combining the nano-silver particles wrapped by the chitosan with the beta-cyclodextrin modified carbon quantum dots.

The invention discloses a method for preparing mushroom peptide with salt reducing and salinity increasing capabilities by a controllable biological enzymolysis technology, which comprises the following steps: (1) dissolving edible mushroom powder in water, uniformly stirring, heating and cooling to room temperature; (2) adding cellulase into the solution obtained in the step (1) for enzymolysis, and finishing the first-stage enzymolysis; (3) adding flavourzyme into the enzymatic hydrolysate obtained in the step (2) for enzymolysis, and finishing second-stage enzymolysis; (4) adding alkaline protease into the enzymatic hydrolysate obtained in the step (3) for enzymolysis, and ending third-stage enzymolysis; and (5) carrying out enzyme deactivation on the enzymatic hydrolysate obtained in the step (4), cooling to room temperature, centrifuging, and taking the supernatant to obtain the mushroom protein peptide which is rich in protein peptide and has an obvious salty-increasing effect as the mushroom peptide with salt-reducing and salty-increasing capabilities.

The invention relates to the technical field of food production, in particular to highland barley dietary fiber and a preparation method thereof.The preparation method comprises the steps that highland barley is subjected to steam explosion, drying, grinding and screening in sequence to obtain a highland barley material, and when it is judged that preparation of the highland barley material does not meet a preset standard according to an explosion characteristic value, the highland barley dietary fiber is obtained; secondarily judging whether the preparation of the highland barley material meets the preset standard or increasing the steam explosion pressure of the steam explosion machine; preparing a highland barley suspension from a highland barley material meeting a preset standard, sequentially adding a high-temperature-resistant alpha-amylase solution, an alkaline protease solution and a saccharifying enzyme into the highland barley suspension to obtain a highland barley saccharification liquid, and judging whether the preparation of the highland barley saccharification liquid meets the preset standard or not according to the enzymolysis characteristic value; the ethanol solution is added into the highland barley saccharified liquid meeting the preset standard for centrifugal operation, the obtained precipitate is the highland barley dietary fiber, and whether the preparation of the highland barley dietary fiber meets the preset standard or not is judged according to the light absorption characteristic value, so that the quality of the highland barley dietary fiber is improved.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention relates to a preparation method and application of yolk polypeptide with ACE inhibitory activity, and the method comprises the following steps: Q1, adding degreased yolk powder into a proper amount of water, dissolving, and adjusting the pH value to 6-8; and Q2, adding alkaline protease or flavourzyme to carry out enzymolysis for 1.5-2.5 hours under the conditions that the temperature is 50-60 DEG C and the pH value is 7-8, and then carrying out enzyme deactivation to obtain the egg yolk hydrolysate. The ACE inhibitor with high activity is prepared by adopting step-by-step enzyme hydrolysis, five ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, APTFGKL and KLPDMILY) with high activity are screened and identified by utilizing a bioinformatics method, and in order to solve the problem that the polypeptide is unstable after being sterilized in the functional beverage, CMC and GG are used as stabilizers, so that the utilization problem of the enzymolysis polypeptide in the functional beverage is solved.

The invention relates to the technical field of gel material preparation, in particular to a bio-based gel material for dangerous chemical leakage disposal and a preparation method thereof.The preparation method comprises the steps that gelatin and deionized water are added into a reaction container, a gelatin solution is obtained under the preset dissolution condition, alkaline protease is added into the gelatin solution for an enzymolysis reaction, and a gelatin solution is obtained; a modified gelatin solution is obtained; the preparation method comprises the following steps: adding a phytic acid-chitosan compound and magnesium-aluminum layered double hydroxide into dispersion equipment, and obtaining a composite inorganic matter dispersion liquid under a preset dispersion condition; adding the modified gelatin solution, the composite inorganic substance dispersion liquid, polyethylene glycol and phase change microcapsules into a mixing reactor, and obtaining a premixed solution under a preset mixing condition; sequentially adding an initiator, a cross-linking agent and tetramethylethylenediamine into the premixed liquid, and performing cross-linking reaction under preset cross-linking conditions to obtain a bio-based gel initial product. The plugging efficiency of the bio-based gel material on dangerous chemical leakage is improved.

The invention relates to the technical field of fermentation extraction, and particularly provides a PHA extraction and purification method, a PHA product and application, the PHA extraction and purification method comprises the following steps: (1) adding a surfactant I into a PHA fermentation broth, stirring while keeping the temperature, adding alkali until the feed liquid is alkaline, and carrying out mechanical wall breaking treatment to obtain a first feed liquid; (2) adding lysozyme into the first feed liquid, keeping the temperature, adding alkali until the feed liquid is alkaline, adding alkaline protease, keeping the temperature, and obtaining second feed liquid; and (3) adding a surfactant II into the second feed liquid, stirring and washing, wherein the surfactant II comprises SDS (sodium dodecyl sulfate), sodium lauryl sulfate and lauryl glucoside. The PHA product obtained by adopting the PHA extraction and purification method has excellent purity and whiteness, so that the PHA product has a wider application prospect, and the method is simple in process, convenient to operate and low in cost.

The invention discloses a production method of full-fishy-smell-removed soybean milk powder, and relates to the technical field of food, and the production method comprises the following specific steps: (1) soaking: soaking soybeans in white vinegar for 14-16 days, and grinding to obtain coarse soybean milk; (2) deodorization treatment: adding a compound enzyme into the coarse soybean milk for enzymolysis and deodorization to obtain an enzymatic hydrolysate, and then sequentially adding beta-cyclodextrin and activated carbon into the enzymatic hydrolysate for deodorization treatment to obtain deodorized soybean milk; wherein the compound enzyme is composed of alkaline protease, flavourzyme and lipase; and (3) sequentially homogenizing, sterilizing and spray-drying the de-fishy soybean milk to obtain the fully de-fishy soybean milk powder. Through combined application of multiple steps and multiple technologies such as soaking pretreatment, compound enzymolysis deodorization, beta-cyclodextrin inclusion and activated carbon adsorption, the beany flavor and other peculiar smell components of soybeans can be thoroughly removed, so that the produced soybean milk powder has pure and fresh flavor, and the taste and market acceptability of the product are greatly improved.

The invention provides a preparation method and application of anti-aging and cell-repairing pancreas biological zymolyte, and relates to the technical field of enzyme viscera extracts. The preparation method comprises the following steps: pretreating pancreas, adding water, pulping, boiling, cooling to room temperature, and adjusting the pH value; adding alkaline protease, heating for enzymolysis, dynamically adjusting the temperature and pH during enzymolysis, and inactivating after enzymolysis is finished to obtain enzymatic hydrolysate; adding a reducing agent into the enzymatic hydrolysate, then adding staphylococcus xylosus liquid, carrying out two-stage fermentation, taking supernate after fermentation is finished, and carrying out ultrafiltration concentration; the staphylococcus xylosus bacterial liquid is produced from staphylococcus xylosus YB-12, the staphylococcus xylosus YB-12 is preserved in the China General Microbiological Culture Collection Center on January 3, 2023, and the preservation number of the staphylococcus xylosus YB-12 is CGMCC No. 26373. The finished product prepared by the method is high in stability; meanwhile, the zymolyte can be applied to cell repair or anti-aging.

The invention discloses a preparation process of environment-friendly degradable thermo-sensitive paper for food, and relates to the technical field of thermo-sensitive paper. Wood pulp and polylactic acid fibers are pulped, lignin is used for backfilling, so that interaction among the fibers is enhanced, pores of prepared paper are reduced along with improvement of the pulping degree, a matrix indirectly achieves the oil-proof effect, then the interior of the matrix is in a porous form through vacuum freeze drying treatment, and under assistance of ultrasonic waves, the oil-proof effect of the matrix is improved. A starch composite zein protein solution is introduced to realize an easy degradation effect, and alkaline protease is utilized to hydrolyze zein protein and react with glucose, so that a dried protein structure becomes compact, a flexible film layer structure is formed on the surface of a matrix, and the transmittance of water molecules and grease molecules is reduced; the easily degradable and oil-proof effects are further improved.

The invention relates to a production method of imitated natural casings. The production method comprises the following steps: selecting high-quality cow belly and cow back split leather and leftover materials of natural casings; the split leather is treated with alkaline protease in a rotating hub, ammonium sulfate and citric acid are used for decalcification treatment after treatment, and citric acid and sodium citrate are used for buffering; fully cleaning; aging and fully swelling; mixing split leather and natural casing leftover materials, and circularly grinding to obtain collagen pulp; mixing the mucilage and acid water; the colloid is fully swelled; homogenizing by a homogenizer, and filtering to obtain collagen colloid; and extruding the imitated natural collagen casing. The method has the advantages that split leather is screened, the casing is softer and more stable in quality, the raw materials comprise leftover materials of natural casings, and the performance of the casing is guaranteed; high-speed extrusion is adopted, so that the strength during filling is ensured, and the casing can be brighter; drying is rapid, so that the casing has enough brittleness; and a gradual traction mode is used, so that the softness similar to that of natural casings is ensured.