316 results about "Alkaline protease" patented technology

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention relates to the technical field of microorganisms, and particularly discloses a fermentation method of bifidobacterium longum BBMN68 as well as a fermented product, a postbiotic and application of the fermented product. According to the method for fermenting the bifidobacterium longum BBMN68, a fermentation substrate comprises alkaline protease hydrolyzed milk and a pomelo peel extract; the hydrolysis rate of the alkaline protease hydrolyzed milk is 9-11%, and the water-soluble dietary fiber content of the shaddock peel extract is 75-80%; the mass ratio of the alkaline protease hydrolyzed milk to the shaddock peel extract is (10-15): (1-3). The content of active ingredients in the fermented product prepared by the method is high, and after the fermented product is prepared into the postbiotics to be taken, the immune function of an organism can be recovered, immune organ damage is reduced, inflammation is relieved, and intestinal flora is regulated and controlled.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention belongs to the field of egg by-product processing, and particularly relates to a preparation method and application of eggshell membrane peptides, the preparation method of the eggshell membrane peptides comprises the following steps: S1, drying and crushing cleaned eggshells, and then separating eggshell membranes; s2, putting the eggshell membrane into a steam explosion machine, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.5 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; s3, adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-9 according to the solid-liquid ratio of 1: (20-30), then adding alkaline protease according to the enzyme addition amount of 5000-15000U / g, carrying out enzymolysis for 5-10 hours at the temperature of 35-65 DEG C, then inactivating for 3-5 minutes at the temperature of 100 DEG C, filtering enzymatic hydrolysate, taking filtrate, centrifuging, taking supernate, and freeze-drying to obtain polypeptide; s4, polypeptide obtained in S3 is dissolved in an activation solution to be activated for 8-10 h at the temperature of 4-8 DEG C; and S5, separating the sample activated in the step S4 through a Sephadex G-15 chromatographic column to obtain the activated eggshell membrane peptide. Based on the method, the preparation efficiency is high, and the prepared eggshell membrane peptide is high in antioxidant activity.

The present application relates to the technical field of oat bran deep processing, and particularly relates to an oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, and a preparation method and application thereof.The present application is prepared by adding alkaline protease and ficin for double-enzyme step-by-step hydrolysis after homogenate, enzymolysis and alkali extraction and acid precipitation pretreatment, and then filtering, ultrafiltration desalination and drying, so that the oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity is obtained.The present application is simple to operate, low in equipment requirement, and suitable for industrial large-scale production.The prepared oat active polypeptide has GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, has a significant inhibitory effect on pancreatic lipase and alpha-glucosidase, has an obvious GLP-1 secretion promotion effect, and has a significant blood sugar reduction and lipid reduction effect.

The invention discloses a preparation method of black garlic polysaccharide, which comprises the steps of extraction, enzymolysis deproteinization, ultrafiltration, concentration, drying, crushing and the like, and'enzymolysis deproteinization + ultrafiltration 'is the core step of the method. Wherein the enzymolysis deproteinization can be selected from papain, neutral protease, acid protease, alkaline protease and the like or a combination of the papain, the neutral protease, the acid protease and the alkaline protease. In the ultrafiltration step, an organic ultrafiltration membrane or an inorganic ceramic membrane or a combination of the organic ultrafiltration membrane and the inorganic ceramic membrane can be selected. The method has the remarkable advantages of simple process flow, low cost, safety, high efficiency, good finished product quality and the like. Pharmacological experiments prove that the black garlic polysaccharide prepared by the method disclosed by the invention has a remarkable anti-constipation effect, and can be used for preparing anti-constipation healthy products, including medicines, health-care foods and foods.

The invention relates to the technical field of active peptides, in particular to a myocardial active peptide capable of relieving heart failure and protecting heart and a preparation method thereof. The method takes pig hearts as raw materials, and sequentially comprises the following steps: pretreating the pig hearts, adding water, and homogenizing; adding alkaline protease to carry out first constant-temperature enzymolysis; adding neutral protease and flavourzyme to carry out second constant-temperature enzymolysis; then lipase is added for third-time constant-temperature enzymolysis, and enzyme deactivation and cooling are carried out; and finally, adding activated carbon into the enzymatic hydrolysate, filtering with diatomite, and spray-drying to obtain the myocardial active peptide. According to the method, the yield and the purity of the active peptide are remarkably improved through a synergistic process of three-step enzymolysis and activated carbon purification, the obtained product is rich in small molecule peptide with a definite heart protection function, and a new way is provided for high-value utilization of pig heart resources and development of functional products for preventing and treating heart failure.

The invention discloses mandulapalka milk and a preparation method thereof, and belongs to the technical field of beverage processing. According to the technical scheme provided by the invention, the preparation method of the degreased cyperus esculentus milk enzymolysis beverage comprises the following steps: adding high-temperature amylase into a degreased cyperus esculentus meal aqueous solution for enzymolysis for 1 hour, and then adding alkaline protease and flavourzyme for enzymolysis for 1 hour to obtain an enzymolysis solution; and finally, emulsifying residual starch and dextrin in the enzymatic hydrolysate by using soybean protein isolate and corn oil, and homogenizing by using a high-pressure homogenizer to obtain the cyperus esculentus beverage. According to the method, a multi-enzymolysis technology is adopted, the enzymolysis speed can be increased, the taste can be improved, a double-enzymolysis method is adopted, the enzymolysis time is shortened, starch is subjected to enzymolysis more sufficiently, and the flavor is improved. The defatted cyperus esculentus beverage prepared by the method disclosed by the invention is appropriate in sweetness, silky in mouth, mellow and strong in fragrance and unique in flavor, and is a healthy and mellow leisure beverage.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.

The invention discloses a method for preparing mushroom peptide with salt reducing and salinity increasing capabilities by a controllable biological enzymolysis technology, which comprises the following steps: (1) dissolving edible mushroom powder in water, uniformly stirring, heating and cooling to room temperature; (2) adding cellulase into the solution obtained in the step (1) for enzymolysis, and finishing the first-stage enzymolysis; (3) adding flavourzyme into the enzymatic hydrolysate obtained in the step (2) for enzymolysis, and finishing second-stage enzymolysis; (4) adding alkaline protease into the enzymatic hydrolysate obtained in the step (3) for enzymolysis, and ending third-stage enzymolysis; and (5) carrying out enzyme deactivation on the enzymatic hydrolysate obtained in the step (4), cooling to room temperature, centrifuging, and taking the supernatant to obtain the mushroom protein peptide which is rich in protein peptide and has an obvious salty-increasing effect as the mushroom peptide with salt-reducing and salty-increasing capabilities.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention relates to a preparation method and application of yolk polypeptide with ACE inhibitory activity, and the method comprises the following steps: Q1, adding degreased yolk powder into a proper amount of water, dissolving, and adjusting the pH value to 6-8; and Q2, adding alkaline protease or flavourzyme to carry out enzymolysis for 1.5-2.5 hours under the conditions that the temperature is 50-60 DEG C and the pH value is 7-8, and then carrying out enzyme deactivation to obtain the egg yolk hydrolysate. The ACE inhibitor with high activity is prepared by adopting step-by-step enzyme hydrolysis, five ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, APTFGKL and KLPDMILY) with high activity are screened and identified by utilizing a bioinformatics method, and in order to solve the problem that the polypeptide is unstable after being sterilized in the functional beverage, CMC and GG are used as stabilizers, so that the utilization problem of the enzymolysis polypeptide in the functional beverage is solved.

The invention relates to the technical field of gel material preparation, in particular to a bio-based gel material for dangerous chemical leakage disposal and a preparation method thereof.The preparation method comprises the steps that gelatin and deionized water are added into a reaction container, a gelatin solution is obtained under the preset dissolution condition, alkaline protease is added into the gelatin solution for an enzymolysis reaction, and a gelatin solution is obtained; a modified gelatin solution is obtained; the preparation method comprises the following steps: adding a phytic acid-chitosan compound and magnesium-aluminum layered double hydroxide into dispersion equipment, and obtaining a composite inorganic matter dispersion liquid under a preset dispersion condition; adding the modified gelatin solution, the composite inorganic substance dispersion liquid, polyethylene glycol and phase change microcapsules into a mixing reactor, and obtaining a premixed solution under a preset mixing condition; sequentially adding an initiator, a cross-linking agent and tetramethylethylenediamine into the premixed liquid, and performing cross-linking reaction under preset cross-linking conditions to obtain a bio-based gel initial product. The plugging efficiency of the bio-based gel material on dangerous chemical leakage is improved.

The invention relates to the technical field of fermentation extraction, and particularly provides a PHA extraction and purification method, a PHA product and application, the PHA extraction and purification method comprises the following steps: (1) adding a surfactant I into a PHA fermentation broth, stirring while keeping the temperature, adding alkali until the feed liquid is alkaline, and carrying out mechanical wall breaking treatment to obtain a first feed liquid; (2) adding lysozyme into the first feed liquid, keeping the temperature, adding alkali until the feed liquid is alkaline, adding alkaline protease, keeping the temperature, and obtaining second feed liquid; and (3) adding a surfactant II into the second feed liquid, stirring and washing, wherein the surfactant II comprises SDS (sodium dodecyl sulfate), sodium lauryl sulfate and lauryl glucoside. The PHA product obtained by adopting the PHA extraction and purification method has excellent purity and whiteness, so that the PHA product has a wider application prospect, and the method is simple in process, convenient to operate and low in cost.

The invention provides a preparation method and application of anti-aging and cell-repairing pancreas biological zymolyte, and relates to the technical field of enzyme viscera extracts. The preparation method comprises the following steps: pretreating pancreas, adding water, pulping, boiling, cooling to room temperature, and adjusting the pH value; adding alkaline protease, heating for enzymolysis, dynamically adjusting the temperature and pH during enzymolysis, and inactivating after enzymolysis is finished to obtain enzymatic hydrolysate; adding a reducing agent into the enzymatic hydrolysate, then adding staphylococcus xylosus liquid, carrying out two-stage fermentation, taking supernate after fermentation is finished, and carrying out ultrafiltration concentration; the staphylococcus xylosus bacterial liquid is produced from staphylococcus xylosus YB-12, the staphylococcus xylosus YB-12 is preserved in the China General Microbiological Culture Collection Center on January 3, 2023, and the preservation number of the staphylococcus xylosus YB-12 is CGMCC No. 26373. The finished product prepared by the method is high in stability; meanwhile, the zymolyte can be applied to cell repair or anti-aging.

The invention relates to the technical field of colla corii asini peptide preparation, in particular to a preparation method of colla corii asini peptide. The preparation method comprises the following steps: step 1, unhairing and degreasing fresh donkey hide, dicing, blanching and cleaning to obtain blocky donkey hide; step 2, adding the blocky donkey hide into water for ultrasonic treatment, then cooking, filtering and collecting filtrate 1; and step 3, adding a compound enzyme into the filtrate 1, carrying out ultrasonic treatment, sequentially carrying out enzymolysis, enzyme deactivation and centrifugal filtration, carrying out debitterizing, decolorizing and degreasing treatment on the obtained filtrate 2, carrying out ultrafiltration, and carrying out spray drying to obtain the colla corii asini peptide, the compound enzyme is a mixture of alkaline protease, neutral protease, pepsase and papain in a mass ratio of 3: 1: 2: 1. According to the method, the donkey-hide gelatin peptide is prepared from the fresh donkey hide, the production cost is reduced, and the donkey-hide gelatin peptide with the molecular weight smaller than 1000 Daltons in the donkey-hide gelatin peptide prepared through the method is high in proportion and can be easily absorbed and utilized by the human body.

The invention relates to the technical field of treatment of thallus mash, in particular to a treatment method and system of thallus protein mash and application of the treatment method and system. The treatment method comprises the following steps: carrying out vacuum concentration on mycoprotein mash to obtain concentrated protein mash; carrying out wall breaking treatment on the concentrated protein mash to obtain concentrated protein mash containing thallus fragments; carrying out neutralization reaction on the concentrated protein mash by using an alkaline solution to obtain a concentrated protein reaction solution; sequentially carrying out enzymolysis reaction and enzyme deactivation treatment on the concentrated protein reaction solution by using a compound enzyme system to obtain a proteolysis reaction solution; wherein the compound enzyme system comprises the following raw materials: lysozyme, alkaline protease, nuclease and flavourzyme; and carrying out purification treatment on the proteolysis reaction liquid to obtain the mycoprotein treatment liquid. According to the treatment method, operations such as vacuum treatment, wall breaking treatment, neutralization reaction treatment and enzymolysis reaction are combined, and the content of peptide substances in the mycoprotein mash is effectively increased to 25% or above.

The invention relates to the technical field of laundry detergents, and discloses a laundry detergent containing a modified gemini quaternary ammonium salt surfactant and a preparation method thereof.The laundry detergent is prepared from iminodiacetonitrile, cardanol glycidyl ether, 1, 8-dibromooctane, Raney Ni, 1, 3-propane sultone, brominated alkane and the like as raw materials through a one-step method. The modified gemini quaternary ammonium salt surfactant is obtained through an epoxy ring-opening reaction, a quaternization reaction, a reduction reaction, a ring-opening alkylation reaction and a substitution reaction. The preparation method comprises the following steps: adding alkyl glycoside and the modified gemini quaternary ammonium salt surfactant into deionized water, uniformly stirring and mixing, adding alkaline protease and the like, and stirring and mixing to obtain the laundry detergent containing the modified gemini quaternary ammonium salt surfactant. Tests prove that the laundry detergent prepared by the invention has better dirt-removing power and antibacterial effect.

The invention provides a low-sensitization whey protein hydrolysate as well as a preparation method and application thereof, and the preparation method of the low-sensitization whey protein hydrolysate comprises the following steps: adjusting the pH value of whey liquid to be alkaline, then adding protease, and carrying out heat-preservation hydrolysis, the protease is selected from at least one of bacillus licheniformis alkaline protease, aspergillus oryzae neutral protease and aspergillus mellitus protease. At least one of the bacillus licheniformis alkaline protease, the aspergillus oryzae neutral protease and the honey aspergillus protease is adopted to hydrolyze the whey liquid, unique active ingredients of protein hydrolysate can be enriched, and therefore the problem that existing protein hydrolysate is high in sensitization is effectively solved, excessive hydrolysis is avoided, and the product quality is improved. The nutritional value of protein is reserved, the crowd acceptability is high, the problem that special crowds are allergic or intolerant to cow milk protein is effectively solved, the method is simple, the raw materials are easy to obtain, and the cost is low.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention discloses a preparation method of a crocodile polypeptide extract and a beverage, and relates to the technical field of polypeptide extraction and beverage preparation, and the preparation method comprises the following steps: pretreatment of crocodile skin; denaturation treatment: adding a NaHCO3 buffer solution, and treating under a microwave condition; performing ultrasonic pretreatment; carrying out enzymolysis reaction, namely sequentially carrying out primary enzymolysis of alkaline protease and secondary enzymolysis of compounding trypsin and neutral protease; adding composite colloid after enzyme deactivation treatment, wherein the composite colloid comprises flaxseed gum and xanthan gum; centrifuging to take supernate, and carrying out fractional separation through ultrafiltration membranes with the molecular weight cut-off of 500Da and 1000Da; and concentrating and drying to obtain polypeptide powder. According to the method, the polypeptide yield, the beverage stability and the oral bioavailability are improved; finally, the obtained polypeptide is scientifically matched with hyaluronic acid and vitamin C to prepare the beautifying beverage with the effects of remarkably improving skin stains and fine wrinkles and brightening the skin color, and the breakthrough of high-value utilization of crocodile skin resources and product function integration is realized.

This invention discloses a reconstituted rice made from leafy greens and its preparation method. The method involves mixing brown rice flour pretreated with leafy green filtrate with leafy green residue obtained by treatment with sodium hydroxide and alkaline protease. The mixture is then extruded, molded, and air-dried to obtain high-quality, high-nutrient reconstituted rice made from leafy greens. This method solves the problem of nutrient loss in grain raw materials without adding other additives, while synergistically promoting nutrient absorption, improving raw material utilization and nutrient absorption efficiency, and enhancing the nutritional value of the reconstituted rice.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

The invention relates to the technical field of daily chemicals, in particular to a color-protecting laundry detergent containing plant extracts and a preparation method of the color-protecting laundry detergent. The color-protecting laundry detergent is prepared from the following components in percentage by mass: 12.5 to 18.3 percent of natural anionic surfactant, 6.4 to 9.7 percent of nonionic surfactant, 3.2 to 5.8 percent of plant polyphenol extract, 3.0 to 5.2 percent of fiber affinity natural polymer, 2.1 to 4.0 percent of bio-based chelating aid, 1.8 to 3.5 percent of natural glycoside synergist, 0.9 to 2.3 percent of pH buffering agent, 0.6 to 1.4 percent of antioxidant plant essential oil and the balance of deionized water. The compound protease comprises alkaline protease, neutral protease and papain; the invention aims to solve the technical problems that the existing laundry detergent is poor in color protection effect, insufficient in environmental protection performance and large in damage to fabrics, and provides a laundry detergent product which takes natural plant extracts as core components and has an efficient color protection function.

The application provides a preparation method of non-denatured type II collagen for increasing bone density and improving bone elasticity, and belongs to the technical field of biotechnology. The application adopts EDTA-citric acid buffer containing beta-mercaptoethanol to decalcify cartilage, adopts supercritical CO2 extraction technology to defat, adopts alkaline protease and collagenase II to perform matrix directional enzymolysis, and performs gradient digestion; then three-phase centrifugal separation is adopted to separate non-denatured type II collagen, and biomimetic H column chromatography purification is adopted, so that non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained.

The application discloses a kind of S-delta-substituted caprolactam chemical / enzyme series preparation method, and the specific preparation method is: 1-substituted high allyl amine occurs dynamic kinetic resolution reaction with acrylic ester compound under the catalysis of alkaline protease (special treatment) and generates S-acryl (1-substituted high allyl) amine, then the product occurs intramolecular olefin metathesis under the catalysis of Grubbs reagent and finally generates S-delta-substituted caprolactam shown in formula (1), wherein, substituent R is phenyl, halogenated phenyl or C2-C12 alkyl.The application has mild reaction condition, is conducive to reducing the equipment requirement of preparation process, improves synthesis safety and economy;Secondly, the reaction course of the method is simple, and single configuration S-delta-substituted caprolactam can be obtained by one-pot reaction, with very high atom economy.

The invention belongs to the technical field of bioengineering, and particularly provides a stable liquid alkaline protease preparation which comprises liquid alkaline protease, NaCl, polysaccharide and histidine. Based on the mass of the liquid alkaline protease, the addition amount of NaCl is 5%-10%, the addition amount of polysaccharide is 0.1%-0.5%, and the addition amount of histidine is 0.02%-0.1%. According to the stable liquid alkaline protease preparation provided by the invention, NaCl, polysaccharide and histidine are added into alkaline protease, filtration and sterilization are performed after pH is adjusted, the stability of the liquid alkaline protease is obviously improved, heat preservation is performed for 6 months at the temperature of 45 DEG C, and the enzyme activity is basically free of loss. The preparation has simple components and is especially suitable for feed and food industries.

The application discloses a preparation method and application of Lactobacillus plantarum fermentum based on environmental stress, and comprises the following steps: activating Lactobacillus plantarum; inducing and culturing the Lactobacillus plantarum in a culture medium containing sodium dodecyl sulfate to obtain Lactobacillus plantarum under environmental stress; dissolving milk powder in deionized water, adding alkaline protease, adjusting the pH to 7-10, and performing enzymolysis to prepare an enzymolysis solution; mixing the enzymolysis solution with sugar to obtain a fermentation substrate; inoculating the Lactobacillus plantarum into the fermentation substrate, performing fermentation culture, centrifuging to collect supernatant, sterilizing, and obtaining the Lactobacillus plantarum fermentum under environmental stress. It is found for the first time that sodium dodecyl sulfate stress can promote the synthesis of anti-inflammatory peptides of Lactobacillus plantarum, and the efficacy threshold of a natural strain is broken. The method is mild in conditions, simple in operation, is conducive to industrialized production, and fully guarantees the stability of product quality.