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Method for extracting rice protein powder by utilizing enzyme-alkali method

A technology of rice protein powder and enzyme base, which is applied in the food field, can solve the problems of unsatisfactory water solubility and functional properties, imperfect extraction methods, and limited application, and achieve easy control of product quality, short heating time, and simple process Effect

Inactive Publication Date: 2010-10-27
长沙湘博医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the amount and demand of rice protein is increasing day by day, its application in the food industry is very limited, mainly due to its imperfect extraction method, water solubility and its functional properties are not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 100g of rice dregs powder crushed through a 100-mesh sieve, add water according to the ratio of 1g of rice dregs powder to 8ml of water, adjust the temperature to 80°C, keep it warm for 1.5h, wash it once, pour it into a colloid mill and refine it for 30s. Centrifuge at 5000r / min for 10min to take the precipitate; add water at the ratio of 1g of precipitate to 8ml of water, adjust the pH to 4.5, and add high temperature resistant α-amylase at the rate of 1g of precipitate plus 150u enzyme activity For amylase, heat up to 90°C, keep warm for 90min, then centrifuge at 5000r / min for 10min to get the precipitate; then add water at the ratio of 1g of precipitate to 6ml of water, adjust the pH value to 7.5, at 45°C, press 1g Add alkaline protease to the ratio of precipitate plus 1200u enzyme activity alkaline protease, extraction time is 150min, after enzymolysis is completed, use 1mol / L NaOH solution to adjust the pH of the system to 12 for alkaline extraction, extractio...

Embodiment 2

[0023] Take 100g of rice dregs powder crushed through a 100-mesh sieve, add water according to the ratio of 1g of rice dregs powder to 10ml of water, adjust the temperature to 90°C, keep it warm for 1h, wash it twice, refine it with ultrasonic waves for 60s, and then use it at 4000r / min. Centrifuge under the condition of 15min to take the precipitate; then add water according to the ratio of 1g sediment to 10ml water, adjust the pH to 4.6, and add high temperature resistant alpha amylase according to the ratio of 1g sediment plus 120u enzyme activity of high temperature resistant alpha amylase, heat up To 95°C, keep warm for 60min, then centrifuge at 4000r / min for 15min to get the precipitate; then add water according to the ratio of 1g of precipitate to 8ml of water, adjust the pH value to 8.0, at 50°C, add 1000u to 1g of precipitate Add alkaline protease to the proportion of alkaline protease with enzyme activity, and the extraction time is 120min. / min, centrifuge under the...

Embodiment 3

[0025] Take 100g of rice dregs powder crushed through a 100-mesh sieve, add water according to the ratio of 1g of rice dregs powder to 9ml of water, adjust the temperature to 85°C, keep it warm for 1.2h, wash it once, pour it into a colloid mill and refine it for 45s, and then Centrifuge at 4500r / min for 12min to take the precipitate; then add water at the ratio of 1g of precipitate to 9ml of water, adjust the pH to 4.5, and add high temperature resistant alpha amylase at the ratio of 1g of precipitate to 140u enzyme activity For amylase, heat up to 92°C, keep warm for 75min, then centrifuge at 4500r / min for 12min to get the precipitate; then add water at the ratio of 1g of precipitate to 7ml of water, adjust the pH value to 7.8, at 48°C, press 1g Add alkaline protease to the ratio of sediment plus 1100u enzyme activity alkaline protease, and the extraction time is 135min. Centrifuge at 4500r / min for 12min to take the supernatant for 135min, adjust the pH of the supernatant to...

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PUM

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Abstract

The invention provides a method for extracting rice protein powder by utilizing enzyme-alkali method. The method in the invention takes rice residue as raw materials and comprises the following steps: washing with water, eliminating sugar, carrying out milling refining on the colloid, eliminating residue starch by utilizing starch enzyme, pre-extracting alkali protease, adding alkali for extraction, centrifuging and taking the supernate to mix to neutrality, inactivating enzymes and sterilizing, and spraying and drying to obtain the rice protein powder. The rice protein powder produced by utilizing the process has faint yellow color, delicate taste and good solubility, and can be maintained well from physicochemical function. The invention has simple process, few investment device, high yield and purity of products, good solubility, and easily-controlled product quality, is suitable for practice popularization and provides a new route for the extraction of rice protein.

Description

Technical field: [0001] The invention relates to the field of food, in particular to a deep processing technology of rice protein powder which is a high-quality vegetable protein resource used in food production. Background technique: [0002] Rice is an important food crop. According to statistics, about half of the world's population takes rice as a staple food. The main area of ​​rice production and consumption in the world is Asia, which accounts for 91% of the world's total consumption, and my country's rice production accounts for 37% of it. %, ranking first in the world. The by-products in rice processing - broken rice, rice dregs, rice bran, etc. contain many ingredients with great development value. Rice dregs are the by-products of monosodium glutamate, lactic acid, citric acid and starch sugar production in the fermentation industry. They have a good amino acid composition ratio and contain essential amino acids needed by the body, and have great recycling value. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12
Inventor 吴卫国徐伟
Owner 长沙湘博医药科技有限公司
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