13 results about "Alpha-amylase" patented technology

The application discloses a kind of green hydrophobic light-weight bio-based composite material of wood structure imitation and preparation method thereof, comprising the following steps: plant fiber is treated by low-alkali cooking pretreatment, standby;Starch is mixed with water, then 75-95 ℃ is added with ultrasonic vibration stirring 1-2h, then adding mesophilic alpha amylase is carried out enzymolysis, after enzymolysis is completed, oxidation treatment is carried out, then urea is added to starch, stirring reaction 1-2h, finally foaming agent is added, uniformly stirred, and the foaming hot melt modified starch is prepared;Wood pulp fiber, lignin, pretreated plant fiber and foaming hot melt modified starch are mixed according to proportion, and mixed slurry is obtained;The mixed slurry is injected in injection molding machine, and the intermediate bubble layer is prepared;A layer of waterproof film containing lignin nanocellulose slurry and polylactic acid mixed solution is sprayed on the surface of the intermediate bubble layer, and the dense waterproof layer is prepared, that is, obtained.

The invention discloses a production process for preparing citric acid by taking degermed corn flour as a raw material, which comprises the following steps: after corn is subjected to degerming, germs, fibers and a mixture of starch and protein are separated, the mixture of starch and protein is crushed, metered and subjected to size mixing, then primary liquefaction and secondary liquefaction are sequentially performed, and alpha amylase is inoculated; one part of the secondary liquefied liquid serves as a fermentation substrate of a seed tank and enters a fermentation tank together with seed liquid of the seed tank, the other part of the secondary liquefied liquid enters a horizontal screw separation unit, syrup separated by a horizontal screw also enters the fermentation tank, citric acid discharged from the fermentation tank is subjected to primary filter pressing, and citric acid is discharged from the fermentation tank; performing ceramic membrane filtration, pre-ion exchange and flash deoxidation on the primary filter pressing liquid in sequence, then performing chromatographic separation, feeding a purified phase after chromatographic separation into a continuous ion exchange unit for impurity removal, and performing pre-concentration, evaporative crystallization and drying on a discharged material of the continuous ion exchange unit in sequence to obtain a citric acid finished product. The process can improve the added value of byproducts, improve the yield of citric acid and reduce the unit consumption of energy and materials.

The invention discloses a method for increasing oligosaccharide content in pellet feed for pigs. The method comprises the following steps: mixing a starch raw material with water according to a mass ratio of 1: 1.20; adding medium-temperature alpha amylase and high-temperature-resistant alpha amylase for enzymolysis under the condition of 6872 DEG C; directly mixing an oligosaccharide solution obtained by enzymolysis with raw materials of the pellet feed for the pigs; and carrying out steam conditioning, extrusion granulation and ventilation cooling to obtain the pellet feed containing oligosaccharide. According to the method, the process of preparing the oligosaccharide through enzymolysis of the starch is combined with the production process of the pellet feed, so that a high-energy-consumption drying link in the traditional oligosaccharide production is omitted, the production cost is remarkably reduced, and the reduction amplitude can reach 3537%; the oligosaccharide content in the pellet feed is stabilized at 1.702.60%, the water content is not higher than 13.5%, the pellet feed meets the safe storage standard, and the pellet feed has good industrial application value.

The present invention relates to a composition comprising a combination of enzymes comprising an alpha amylase, a glucanase and / or xylanase, and a lipase and / or phosphodiesterase; the use of the composition as a medicament; the use of the composition in the treatment of oral diseases; methods of treatment comprising administering the composition to an animal; methods of preventing or removing an oral biofilm comprising contacting the oral biofilm with the composition; methods for reducing the risk of oral biofilm formation; a method for removing the formed oral biofilm; the invention also discloses a kit containing the composition.

The present application relates to a kind of spent grain protein peptide and its preparation method and application. Specifically, with beer brewing by-product spent grain as raw material, it is prepared by protease enzymolysis to prepare spent grain enzymolysis oligopeptide, and it is proved by mouse in-vivo experiment that the enzymolysis complex has the function of improving blood sugar, obesity, blood fat. On this basis, from the enzymolysis product, the key blood sugar reducing active peptide segment FPQPPQ, FPQQPPFG, FPQPQQPFP is identified, and it is verified that its alpha amylase inhibition rate is superior to the same concentration of acarbose. The spent grain protein peptide complex and spent grain protein peptide of the present application have the effect of reducing blood sugar, and can be applied to the development of food, health food, medicine and the like.

The invention discloses a compound rice soup preparation process and a preparation method thereof, and relates to the technical field of rice soup preparation. Raw material pretreatment: mixing and soaking rice, coarse cereals and compound components; composite enzymatic hydrolysis: adding alpha amylase, saccharifying enzyme and cellulase for enzymatic hydrolysis; performing low-temperature boiling and concentration: boiling the enzymatic hydrolysate, performing vacuum concentration, and adding probiotics and compound trace elements to obtain a rice soup mixed solution; homogenizing: filling the blended mixed solution into a homogenizer, and homogenizing twice under the condition of 15MPa; through compound raw material proportioning and an enzymolysis process, the content of protein in rice soup is increased by 30-40%, the content of essential amino acid is increased by 25-30%, the content of polysaccharide is increased by 15-20% compared with a traditional process, and various trace elements and functional components are introduced.

The invention discloses corn meal replacement powder capable of promoting growth of intestinal probiotics and a preparation method of the corn meal replacement powder. The corn meal replacement powder is prepared from the following components in percentage by weight: 76.9%-88.5% of enzyme unfrozen dry corn flour, 10%-20% of Chinese yam powder, 0.3%-0.7% of stevioside, 0.4%-0.8% of propylene glycol alginate, 0.4%-0.8% of xanthan gum and 0.4%-0.8% of sodium carboxymethyl cellulose. The preparation method comprises the following steps: mixing the corn flour and water, pulping, and adding anhydrous calcium chloride and alpha-amylase for enzymolysis; carrying out suction filtration on the enzymatic hydrolysate, adding water to wash the precipitate, freeze-drying the obtained precipitate, and crushing to obtain enzyme-unfrozen dry corn flour; all the components are uniformly stirred and mixed according to the proportion. According to the meal replacement powder disclosed by the invention, the corn is used as a main material, starch in the corn flour is enzymatically hydrolyzed by using amylase, energy provided by the meal replacement powder is reduced to the greatest extent, the content of lysine in the added Chinese yam is rich, the nutritional structure of the meal replacement powder is improved, the readjusted taste and mouth feel are given to the meal replacement powder, and the utilization space of the corn is expanded.

The invention belongs to the technical field of food processing, and relates to a preparation method of a Chinese yam fermented beverage and a product thereof, the preparation method comprises the following steps: pulping Chinese yam, gelatinizing, and carrying out enzymolysis treatment by using alpha amylase to obtain a first mixture; and performing mixed fermentation on the first mixture by using streptococcus salivarius subsp. Thermophilus and lactobacillus delbrueckii subsp. Bulgaricus to obtain the fermented beverage. The beverage prepared from Chinese yam as a raw material is extremely easy to layer and precipitate, and starch in the Chinese yam is hydrolyzed by alpha-amylase, so that on one hand, the fermentability of the Chinese yam is enhanced, the fermentation efficiency is improved, and the generation of flavor substances in the fermentation process is promoted; on the other hand, the stability of the product in the shelf life is improved to a certain extent. The prepared product is fresh, cool, soft and rich in nutritional value.

The application discloses a millet intelligent maintenance line construction method which is convenient to identify. The application prepares a millet intelligent maintenance line by transferring a complete set of vectors for creating the millet intelligent maintenance line into wild type millet. The complete set of vectors for creating the millet intelligent maintenance line comprises a fertility gene knockout vector and an intelligent sterility expression cassette vector. The fertility gene knockout vector comprises an sgRNA expression cassette and a Cas9 nuclease expression cassette. The intelligent sterility expression cassette vector comprises an aminoglycoside phosphotransferase expression cassette, a fertility restoration gene expression cassette, a corn alpha amylase expression cassette and a betalain expression cassette. The selfed offspring of the millet intelligent maintenance line prepared by the application can generate 50% of the maintenance line seeds and the sterile line seeds respectively. The red seeds have betalain synthesis and are intelligent maintenance line seeds. The yellow seeds have no betalain synthesis and are intelligent sterile line seeds, and naked eyes can distinguish them.

The invention discloses a modification technology for soybean soluble dietary fibers based on UHP. The modification technology comprises the following steps: pretreating SDF raw materials; extracting soluble dietary fibers from the bean dregs; determining basic physicochemical properties, and determining monosaccharide composition; determining the WHC and the OHC of the SDF; analysis is carried out through a scanning electron microscope (SEM), differential scanning calorimetry (DSC) and X-ray diffraction (XRD); soluble dietary fibers of bean dregs are modified by adopting a UHP technology, and a result shows that WHC, OHC and SC of the bean dreg dietary fibers, the antioxidant capacity, the glucose adsorption capacity, the alpha-amylase inhibition capacity and the alpha-glucosidase inhibition capacity (p < lt >; 0.05), cholesterol adsorption capacity, in-vitro bile salt binding capacity and pancreatic lipase inhibition capacity, which indicate that the soybean dreg soluble dietary fiber adsorption capacity, in-vitro blood sugar reducing activity and in-vitro blood fat reducing property are better.

Disclosed are recombinant endoxylanases that are expressed and exported in high levels in bacteria, in particular Bacillus subtilis. The recombinant endoxylanases are based on an endoxylanase selected from Trichoderma reesei or Bacillus pumilus modified with a signal peptide from B. subtilis alpha amylase (AmyE), B. subtilis levanase (SacC), or B. subtilis YwmC. Accordingly, also disclosed are plasmids for transforming bacteria to express and export such recombinant endoxylanases and the resulting recombinant bacterial strains. The disclosure also encompasses compositions for simultaneously degrading and assimilate cellobiose and xylan comprising a recombinant bacteria engineered to produce xylose from hydrolyzing the agricultural biomass and a xylose assimilator and methods of producing value-added products, for example succinate, from agricultural biomass using such compositions.

The application relates to an enzymatic modification of old eagle tea polysaccharide, and preparation comprises the following steps: step one, old eagle tea polysaccharide is prepared into a solution with a concentration of 10-30 mg / mL; step two, biological enzymes are added into the solution to perform an enzymatic reaction, the biological enzymes are papain, medium-temperature alpha amylase or cellulase; step three, after the enzymatic reaction is completed, inactivation is performed, supernatant is obtained through centrifugation, a Sevag method is used to remove protein, after dialysis, freeze-drying is performed, and the enzymatic modification of old eagle tea polysaccharide is obtained. The enzymatic modification of old eagle tea polysaccharide prepared in the application can significantly improve one or more specific effects of old eagle tea polysaccharide.

The present invention relates to a variant pullulanase, having increased thermo-stability and / or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399, 401, 402, 411, 412, 434, 435, 443, 446, 459, 460, 479, 490, 498, 514, 529, 531, 533, 541, 545, 581, 583, 595, 649, 665, 688, 700, 709, 804, 811 of SEQ ID NO: 1, and optionally a deletion of one or more, e.g., all amino acids at positions 821, 822, 823, 824, 825, 826, 827, and 828, wherein the variant has pullulanase activity, and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5.Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.