The invention relates to the technical field of agricultural
biology, and provides an efficient and stable
canna edulis
germplasm resource in-vitro preservation method which comprises the following steps: step 1, selecting healthy and
pollution-free sterile
canna edulis test-tube plantlets; 2, NAA, BA,
kanamycin,
hygromycin B,
sucrose and
agar are added to a B5 culture medium formula, and a culture medium is obtained; 3, the test-tube plantlets are inoculated into a culture medium, the culture medium is placed in an artificial climate box to be cultured, and subculture is conducted once every 6-8 months (the generations are controlled within 6 generations); 4, in the culture process, the growth condition of the test-tube
plantlet is recorded regularly, and meanwhile, a conventional culture medium without a growth inhibitor is set as a control group. By optimizing a culture medium formula and controlling culture conditions,
excessive growth of test-tube plantlets is effectively inhibited, formation of short and strong seedlings is promoted, and functions of root systems and leaves are enhanced, so that long-term, stable and efficient
germplasm resource preservation is realized, the
transplanting survival rate is remarkably increased, and the method has important application value.