57 results about "Malachite green" patented technology

The application discloses a malachite green label-free proportional biosensor based on multi-dimensional cutting aptamer, which comprises the following steps: (1) a performance-improved malachite green aptamer sequence; (2) a label-free proportional malachite green sensor sequence; and (3) malachite green detection. The biosensor is constructed by using various aptamer sequences, and the ideal sensing effect can be realized through the competition balance principle. The label-free proportional sensor can present a good linear relationship in the range of 5nM-4muM, and has a low detection limit of nanomolar level, which indicates that the sensor has good malachite green detection potential and can meet the application requirements.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The present invention relates to a method and a dual-color detection kit for the rapid and simultaneous detection of MS-222 and malachite green, and a technology for the rapid detection of veterinary drug residues in aquatic products. The invention utilizes red and blue colloidal gold with different particle sizes, combined with specific antibodies to construct different red and blue probes, respectively. The invention utilizes the principle that MG-BSA complete antigen and 3-aminobenzoic acid-BSA antigen coated on the T1 line and the T2 line, respectively, can compete with MG and MS-222 in the sample for binding to the blue probe and the red probe, thereby constructing a dual-color rapid detection test strip for MS-222 and malachite green that is clearly distinguishable to the naked eye and simple to operate.

This invention provides a composite aerogel based on Eucommia ulmoides gum and MXene nanosheets, its preparation method, and its application. The aerogel is prepared by reacting a toluene solution of Eucommia ulmoides gum with Ti3C2T... x MXene aqueous solution was mixed and a water-in-oil microemulsion was formed using a combination of ultrasound-assisted shaking. This allowed MXene nanosheets to self-assemble at the microemulsion droplet interface. Following vertical freezing and freeze-drying processes, a three-dimensional network structure of Eucommia ulmoides gum-Ti3C2T was successfully constructed. x MXene composite aerogel. The preparation method of this invention is mild and requires no chemical cross-linking agents. The resulting composite aerogel exhibits excellent adsorption performance for enrofloxacin, malachite green, Congo red, rhodamine, and kanamycin in water, with adsorption equilibrium times all within 10 minutes, enabling rapid removal of organic pollutants from water. This invention provides a new approach for the high-value utilization of Eucommia ulmoides gum and has promising application prospects in the field of water environment remediation.

This invention discloses a G4-DNA tetrahedral probe electrode, its preparation method, applications, and application methods. This invention utilizes four DNA strands to self-assemble into a DNA tetrahedral structure based on Watson-Crick hybridization. The DNA tetrahedron and G4 strand can be connected to the electrode based on the interaction between thiol groups and the gold electrode, and complementary base pairing. The addition of the DNA tetrahedron, through the construction of a reasonable probe density, further improves the detection sensitivity. This invention utilizes square wave voltammetry for electrochemical detection of malachite green. The content of malachite green can be determined based on the strength of the current signal of its characteristic peak. Rapid detection of malachite green can be achieved in a short time, with a minimum detection concentration of 3.65 μg / kg, showing potential for on-site detection. Six repeated tests using this electrode yielded almost identical detection signals, indicating that the electrode has good reusability and stability.

The present application relates to the technical field of water treatment, in particular to a kind of lemon acid modified bagasse biochar and its preparation method and application in removing malachite green in water.The present application uses bagasse as raw material, uses lemon acid as modifier, prepares the alkali treated lemon acid modified bagasse biochar by high-temperature limited oxygen pyrolysis, and is used to absorb organic dye malachite green in water, and the adsorption capacity can reach 1139 mg·g ‑1 .

The invention discloses a nucleic acid aptamer with synergistically enhanced fluorescence property and affinity, and application of the nucleic acid aptamer in a malachite green label-free proportional biosensor. Specifically, the malachite green aptamer biosensor with excellent detection performance is constructed through a competitive balance principle, and the malachite green aptamer biosensor has a wide detection range and a low detection limit. The nucleic acid aptamer and the biosensor provided by the invention can assist the development of a malachite green detection method and the development of the field of malachite green food safety or environment detection kits, and have certain universality and industrialization potential.

The invention provides a rare earth metal-organic framework material as well as a preparation method and application thereof, and relates to the technical field of fluorescence detection, the general formula of the material is {[Ln4 (THBA) 2 (H2O) 5]. 4H2O} n, Ln is Eu < 3 + > or Tb < 3 + > ions; tHBA is a deprotonated [1, 1 ', 4', 1 ''-terphenyl]-2 ', 3, 3', 5, 5 ', 5''-hexacarboxylic acid ligand; the material belongs to a triclinic system, the space group is P1, the material has a three-dimensional porous frame structure, and each Ln < 3 + > ion is in an eight-coordinate configuration; n > = 1, and n is an integer. The material not only shows high-sensitivity, high-selectivity and quick-response detectability to the harmful substance malachite green, but also successfully realizes conversion from a powder material to a portable device, and has wide application prospects in the fields of environmental monitoring, food safety, information encryption and the like.

The application discloses a metal composite Fenton-like solid catalyst and preparation and application of the metal composite Fenton-like solid catalyst in removal of malachite green and Cr(VI) in wastewater, and belongs to the technical field of degradation and removal of cationic dyes and chromium by adsorption. 2+ -Silica. Compared with liquid chelating agents involved in the existing research, EDDS in the catalyst structure can provide six coordination atoms due to the structure of four carboxyl and diimino groups, and can form a stable metal complex with Co 2+ by virtue of the superior metal chelating characteristics of the hexadentate ligand EDDS. The metal composite solid catalyst prepared by grafting the spacer arm on the silica gel matrix can be separated by filtration and the like, and the subsequent regeneration operation can be performed by selecting a suitable eluent, so that the metal composite solid catalyst has the characteristics of easy separation, regeneration and reusability.

The invention belongs to the technical field of fluorescence detection, and particularly relates to a ratio-dependent up-conversion fluorescence nanoprobe for veterinary drug detection and a preparation method and application of the ratio-dependent up-conversion fluorescence nanoprobe. The invention provides a ratio type up-conversion fluorescent nanoprobe for veterinary drug detection. The ratio type up-conversion fluorescent nanoprobe comprises a core nanoparticle NaGdF4: Yb / Tm, an inner layer shell NaYbF4, a middle layer shell NaGdF4: Yb / Er and an outer layer shell NaYF4 from inside to outside, after the fluorescent probe is incubated with furaltadone and malachite green, the fluorescence intensity is quenched, the selectivity is good, and the fluorescent probe can be used for detecting furaltadone and malachite green; furthermore, as a ratio-type up-conversion fluorescent nanoprobe, the fluorescent nanoprobe can be excited by low-energy near-infrared light, does not cause light damage, has higher biological tissue penetration depth, no background fluorescence interference and higher signal-to-noise ratio, and is a fluorescence detection method for veterinary drug residues with excellent reliability; therefore, the technical problem of low reliability of fluorescence detection of veterinary drug residues in the prior art is solved.

The application belongs to the field of microbial degradation of dyes, and relates to application of deinococcus in degradation of malachite green wastewater. The deinococcus is Deinococcus wulumuqiensis R12. When the initial concentration of malachite green MG is 200 mg / L, the cell concentration of R12 is 8.0 g / L, the reaction is carried out at 30 DEG C and pH 6.0 for 30 min, the degradation rate is 99.80%. When the initial concentration of MG is 1000 mg / L, the degradation rate can reach more than 95% after 1.5 h of reaction. Meanwhile, the R12 has wide adaptability to pH and temperature in degrading malachite green, and can efficiently degrade malachite green MG under the conditions of pH 5.0-10.0 and 10-45 DEG C.

The invention discloses a method for rapidly detecting malachite green in aquatic products, and belongs to the technical field of biological detection. According to the time-resolved fluorescent microsphere immunochromatography kit based on the specific nano-antibody, the nano-antibody is applied to immunochromatography, an optimized pretreatment method is combined, malachite green in aquatic products can be rapidly detected on site, sample pretreatment and detection can be completed within 15 min, the limit of detection (LOQ) is 0.03 mu g / kg, and the time-resolved fluorescent microsphere immunochromatography kit can be used for detecting malachite green in aquatic products. The naked eye line elimination value is 1.6 mu g / kg which is far higher than the detection level in the prior art, and the method has the advantages of high sensitivity, good specificity, simplicity in operation, short detection time and the like, and is suitable for on-site rapid screening of malachite green residues in aquatic products.

This invention provides a method for detecting malachite green veterinary drug residues using SERS based on silver-coated gold nanorods. The method includes: mixing a malachite green standard solution with silver-coated gold nanorods, adding a coagulant to form a mixed solution, and performing Raman spectroscopy to obtain the surface-enhanced Raman scattering (SERS) peaks of the malachite green standard solution; determining the characteristic peaks used to identify malachite green using SERS by assigning the malachite green molecular structure to the Raman peak positions; and determining the linear relationship between the concentration of malachite green and the intensity of the characteristic peaks. The method also involves mixing the sample solution to be tested with silver-coated gold nanorods, adding a coagulant to form a mixed solution, and performing Raman spectroscopy to obtain the peak intensity of the characteristic peaks of malachite green in the sample solution. The concentration of malachite green in the sample solution is calculated based on the linear relationship between the concentration of malachite green and the peak intensity of the characteristic peaks. This invention enables accurate and rapid detection of malachite green in aquatic products, ensuring the safe production of aquatic products.

The invention relates to a fibrous protein ELISA (enzyme-linked immunosorbent assay) detection method based on homing peptide combined with malachite green, which comprises the following steps: S1, synthesizing a template chain and homing peptide to form a sensing probe; s2, adding a to-be-detected solution containing fibrin into an antibody solution to obtain a fibrin-antibody compound, and adding a sensing probe, a first primer and a second primer for incubation; s3, adding a first lock-type probe and a second lock-type probe for catalytic cyclization; s4, carrying out synchronous rolling circle transcription to obtain long-chain RNA respectively containing M1 and M2 sequence fragments; and S5, adding malachite green dye for incubation, and triggering a fluorescence signal. Compared with the prior art, by means of target fibrin mediation, dual recognition of the integrated antibody and the homing peptide on fibrin and signal generation induced by the split type MG-RNA aptamer, background noise is remarkably reduced, and specificity is remarkably improved.

This invention discloses a rapid detection method for leucomall green based on nanozyme oxidation and a method for preparing nanozymes. The specific steps are as follows: (1) Solution preparation: Prepare an acetate-sodium acetate buffer solution, a leucomall green solution, and a nanozyme stock solution for rapid detection of leucomall green; (2) Add the nanozyme stock solution to the acetate-sodium acetate buffer solution and shake well, then add the leucomall green solution, and measure the absorbance of the solution before and after adding leucomall green at 618 nm; (3) Adjust the final concentration of the malachite green solution, the leucomall green solution, and the mixed solution after nanozyme catalysis of leucomall green to 10 μM, and determine the sample under liquid chromatography-tandem mass spectrometry conditions to obtain an ion chromatogram, confirming that the product of nanozyme catalysis of leucomall green is malachite green. The nanozyme catalysis of leucomall green using the rapid oxidation of leucomall green of this invention is simple to operate, rapid and sensitive, low in cost, and highly efficient.

Preparation of nitrogen-sulfur co-doped canna flower-based biochar and a use thereof in the removal of malachite green in wastewater. The method comprises: washing, drying, and pulverizing a collected canna flower, mixing same with a certain amount of phosphoric acid in a beaker, adding an appropriate amount of deionized water, stirring same, loading a feed liquid into a hydrothermal kettle, and then placing same in an oven for hydrothermal treatment to obtain a canna flower-based hydrothermal carbon; uniformly mixing hydrothermal carbon powder with a certain mass ratio of thiourea and molten salt, placing the mixture in a muffle furnace for firing in air, cooling same with the furnace after firing is completed, finally taking out a sample, separating the molten salt, performing acid washing on the biochar, washing same with alcohol, washing same with water, and then drying same to obtain the nitrogen-sulfur co-doped canna flower-based biochar; and using the nitrogen-sulfur co-doped canna flower-based biochar to remove malachite green in wastewater. Compared with conventional carbon materials, the nitrogen-sulfur co-doped carbon material prepared by the method has greatly improved removal efficiency for malachite green in a water body. In addition, the preparation method provides a way to treat a biomass waste, saves costs, and realizes resource utilization of biomass wastes.

This invention belongs to the field of environmental pollution detection and treatment technology, specifically relating to a SiO2@Ag / ZnO flexible substrate and its application in the detection and degradation of malachite green. A SiO2@Ag nanocomposite material is prepared by modifying Ag nanoparticles onto the surface of SiO2 spheres. Further, ZnO is attached to the surface of SiO2@Ag to prepare the composite material SiO2@Ag / ZnO. A SiO2@Ag / ZnO flexible substrate was successfully prepared by attaching SiO2@Ag / ZnO to fabric. This material exhibits detection capability at lower concentrations and demonstrates stable response to concentration changes in SERS signal detection of malachite green, exhibiting high quantitative accuracy and suitability for SERS quantitative analysis. Simultaneously, this material achieves stable degradation of malachite green under light irradiation conditions; the degradation rate gradually increases with prolonged ultraviolet light irradiation time, reaching a maximum degradation rate of 91.1%.

The invention discloses a preparation method of a carboxyl-functionalized porous organic polymer adsorbent and application of the carboxyl-functionalized porous organic polymer adsorbent in the field of dye wastewater. According to the method, dimethyl formal is used as an external cross-linking agent, LNU-80 is synthesized through a Friedel-Crafts coupling reaction, and a hydrophilic group is introduced into an LNU-80 framework, so that the carboxyl functionalized porous organic polymer LNU-80-COOH is obtained. Experimental results show that LNU-80-COOH shows excellent adsorption performance on malachite green, and the adsorption capacity of the LNU-80-COOH under the room temperature condition reaches up to 4375.9 mg g <-1 >. After five times of circulation, the adsorption efficiency of the LNU-80-COOH on the malachite green is still kept at 90% or above. The invention provides a novel method for directionally designing and preparing the porous organic polymer with high adsorption selectivity. The adsorbent has excellent adsorption selectivity and cycling stability, and has a wide development prospect in the field of dye wastewater treatment.

The present application relates to the technical field of immunochromatographic analysis, and discloses a signal enhancer for a colloidal gold immunochromatographic rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, signal amplification is realized by catalytic deposition of silver nanoparticles on the surface of colloidal gold, the present application is simple to operate, is added at one time, and reaction is completed in 5-10 minutes; sensitivity is improved by 5-10 times, specificity is higher than 98%; stability is excellent, 12 months of storage at 4 DEG C; it is compatible with various colloidal gold rapid detection cards; cost is controllable, single detection is increased by 0.3-0.5 yuan, and the present application is especially suitable for super-sensitive detection of small molecule substances in the field of food safety, such as detection limit of malachite green of 0.1 microgram per liter, detection limit of fipronil of 0.5 microgram per liter, and the application range of the colloidal gold immunochromatographic rapid detection card is significantly expanded.

The present invention discloses a method for processing and detecting a sample. The present invention is based on the addition of a phase separator, a purifier and an extractant to respectively realize extraction and oil removal treatment, solves the difficulty of large conflicts in the extraction and oil removal process due to the large difference in the chemical properties of enrofloxacin, malachite green and diazepam, realizes a four-in-one unified sample pretreatment method of chloramphenicol, diazepam, malachite green and enrofloxacin, and the sample is pretreated once. Relative to the sample pretreatment method of a single compound, under the premise of ensuring high sensitivity, the detection time is greatly shortened, and it is subsequently applicable to the on-site rapid large-scale screening test of the colloidal gold method, and the minimum detection limit of each compound is 0.1μg / kg, 0.5μg / kg, 0.5μg / kg and 2μg / kg, respectively, which meets the accuracy and stability requirements of the detection and is convenient for the detection operation of grassroots personnel.

The application discloses a double-emission ratio fluorescent probe RB@Zr-fcu-sti, a preparation method thereof and application thereof in detection of malachite green (MG). The preparation method comprises the following steps: adding Zr-fcu-sti and rhodamine B into anhydrous ethanol, ultrasonic dispersion, stirring reaction at room temperature in dark, centrifugation, washing, and vacuum drying. The double-emission ratio fluorescent probe RB@Zr-fcu-sti is applied to the fluorescence detection of malachite green, and has high sensitivity and fast response. With the increase of the concentration of the sensing system malachite green (MG), the red fluorescence emission of the probe is significantly quenched, while the intensity of the blue fluorescence emission remains basically unchanged, so that the fluorescence color of the sensing system gradually changes from purple to blue. The detection limit of the application to malachite green (MG) is as low as 2.69nM, and thus the application can be applied to high-sensitivity ratio fluorescence sensing of malachite green (MG).

The invention discloses a visible / ultraviolet dual-light synergistically enhanced substrate, a preparation method thereof and application of the visible / ultraviolet dual-light synergistically enhanced substrate in rapid detection of veterinary drugs. According to the method, a gas-liquid interface self-assembly technology is combined with a light welding process, and the heterojunction composite Raman substrate Bi: ZnO-SUSNs of a Bi-doped zinc oxide nano array and urchin-shaped silver nanoparticles is prepared. The Bi: ZnO-SUSNs composite Raman substrate disclosed by the invention has excellent visible light / ultraviolet light dual response characteristics, and the detection sensitivity to target molecules can be greatly improved under dual-light driving by utilizing a PIERS mechanism. According to the method, trace detection of veterinary drugs such as malachite green, crystal violet and furacilin can be realized without complex pretreatment. In addition, the composite substrate also has a photocatalytic degradation function, can realize self-cleaning and reutilization, and has the advantages of low preparation cost, high detection speed, good repeatability and the like.

The invention discloses an application of a malachite green aptamer in low-damage slow-release bacteriostasis and long-acting bacteriostasis. Specifically, the nucleic acid aptamer with improved affinity, luminescence property and stability can slowly release malachite green in the bacteriostasis process, and assists the long-acting and mild bacteriostasis process. The aptamer with improved multi-dimensional performance is applied to the field of slow-release bacteriostasis, can effectively relieve toxic and side effects of a bactericide on non-target microorganisms or host cells, and is beneficial to realizing long-acting control and relieving drug resistance.

The present application relates to the technical field of food quality supervision, and particularly relates to a method for rapidly detecting sulfur dioxide in shrimps based on fading technology of addition reaction. The method comprises the following steps: S1, weighing the sample to be measured, adding pure water for homogenization, centrifuging, and obtaining supernatant, which is used as sample extract; S2, taking a test tube, adding V1 volume of sample extract and V2 volume of acetonitrile, shaking, centrifuging, making the upper liquid clear, and taking the upper liquid as positive reference; V2 / (V1+V2)=40-70%; taking another test tube for synchronous operation to make the upper liquid clear, then adding malachite green reagent dropwise, shaking gently, and standing for 4-10 min; comparing the test tube with the positive reference, and judging the content of sulfur dioxide in the sample to be measured according to the fading of the lake blue in the test tube. The present application uses dynamic fading reaction to replace static colorimetric card colorimetry, and avoids the subjectivity and matrix interference problems of color card method, and is especially suitable for rapid detection of shrimp samples.

The invention belongs to the field of environmental protection, and particularly relates to a modified reed adsorbent, a preparation method and application of the modified reed adsorbent in malachite green adsorption. The modified reed adsorbent obtained by combining calcium oxide with amylase treatment has good adsorption performance when being applied to adsorption of malachite green. Particularly, for a to-be-adsorbed solution with the malachite green concentration of about 10 mg / L, the adsorption rate can reach 90% or above by adjusting the crushing particle size, the adsorption temperature, the addition amount, the pH and the adsorption time of the modified reed adsorbent, meanwhile, the desorption and regeneration capacity is excellent, and the adsorption capacity can still reach 70% or above after three times of desorption and regeneration.

The invention discloses a method for detecting malachite green based on a fluorescence resonance energy transfer principle, a biosensor and a kit, and belongs to the technical field of aquatic product quality safety detection. According to the method, near-infrared light excited lanthanide doped up-conversion nanoparticles (UCNPs) are used as an energy donor, and a nucleic acid aptamer for specifically recognizing malachite green is covalently linked to the surface of the UCNPs to form the nano probe. When the malachite green does not exist in the sample, the aptamer is in a flexible conformation, and the fluorescence of the UCNPs is quenched due to the FRET effect. When malachite green exists in a sample, the aptamer is specifically combined with the malachite green and is subjected to conformational change, so that the spatial distance between the nanoprobe and a quenching agent is increased, the FRET process is blocked, and UCNPs fluorescence is recovered. The qualitative and quantitative analysis of malachite green can be realized by detecting the change of a fluorescence signal. The method has the advantages of high sensitivity, strong specificity, simple operation and rapid response, and is suitable for on-site rapid detection of malachite green residues in aquatic products and aquatic water.

This invention discloses a method for detecting malachite green residues in food based on a ratiometric fluorescent paper-based sensor using titanium carbide quantum dots and biomass carbon quantum dots. First, titanium carbide quantum dots and biomass carbon quantum dots are prepared. Then, a Ti3C2MXene / biomass quantum dot ratiometric fluorescent probe is prepared. Based on this, a Ti3C2MXene / biomass quantum dot ratiometric fluorescent paper-based sensor (filter paper chip) is constructed. A linear equation for the working curve of malachite green concentration is established. Finally, the pretreated sample solution is dropped onto the ratiometric fluorescent filter paper chip. After drying, the paper chip is photographed using a mobile phone under a 365 nm UV analyzer. The RGB values ​​of the paper chip are extracted using smartphone color recognition software. The obtained R value is substituted into the linear equation of the working curve to obtain the malachite green concentration in the sample. The titanium carbide quantum dot / biomass carbon quantum dot ratiometric fluorescent paper-based sensor and malachite green concentration detection method disclosed in this invention are sensitive, accurate, and rapid.

The invention discloses a copper oxide loaded sea stone composite material for water treatment and a preparation method thereof, sepiolite is loaded with copper oxide to obtain composite particles, and the composite material composed of a plurality of composite particles can effectively adsorb malachite green and other cationic dye molecules and has higher cyclic regeneration performance. The CuO active component on the surface of the composite material has good antibacterial performance, and can synergistically realize water purification and bacteriostasis functions.

The invention discloses a method for detecting the RNA encapsulation efficiency of nano-liposome by using malachite green. The method comprises the following steps: S1, preparing a recombinant MGA solution, a blank sample solution, an MG solution and a nano-liposome demulsification solution with different concentrations; s2, preparing a nano liposome-MGA (Melamine Glycolic Acid) wrapping system; s3, respectively incubating the recombinant MGA solutions with different concentrations and the blank sample solution with the MG solution, detecting the fluorescence intensity by using a multifunctional microplate reader, and calculating the theoretical concentrations of the recombinant MGA solutions in different batches according to the fluorescence intensity, and the accuracy and precision of the detection method; and S4, incubating the nano-liposome demulsification liquid and the nano-liposome-MGA wrapping system, detecting the fluorescence intensity of the nano-liposome-MGA wrapping system before and after demulsification by using a multifunctional microplate reader, and calculating the encapsulation efficiency of the nano-liposome to RNA according to the fluorescence intensity. The method has the characteristics of good accuracy, high precision and convenience in operation in RNA content determination.

The application discloses a SERS substrate for detecting malachite green and a preparation method thereof, and belongs to the technical field of Raman spectroscopy.The preparation method of the substrate is as follows: a PET film is preprocessed by sanding to form a micron-level gully structure on the surface of the PET film; the treated PET film is subjected to oxygen plasma treatment to etch nano-protrusions on the surface of the micron-level gully structure and introduce oxygen-containing polar groups on the surface of the PET film; and a silver layer is grown on the treated PET film by a vacuum evaporation method to obtain a SERS substrate with a micro-nano composite structure for detecting malachite green.The method constructs a micro-nano composite structure on the surface of the PET film through the synergistic effect of sanding and oxygen plasma treatment, greatly improves the SERS activity of the subsequently deposited silver layer, significantly enhances the bonding force between the silver layer and the substrate, and enables the substrate to have high sensitivity, high uniformity, excellent mechanical stability and reusability.