33 results about "Malachite green" patented technology

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

This invention provides a composite aerogel based on Eucommia ulmoides gum and MXene nanosheets, its preparation method, and its application. The aerogel is prepared by reacting a toluene solution of Eucommia ulmoides gum with Ti3C2T... x MXene aqueous solution was mixed and a water-in-oil microemulsion was formed using a combination of ultrasound-assisted shaking. This allowed MXene nanosheets to self-assemble at the microemulsion droplet interface. Following vertical freezing and freeze-drying processes, a three-dimensional network structure of Eucommia ulmoides gum-Ti3C2T was successfully constructed. x MXene composite aerogel. The preparation method of this invention is mild and requires no chemical cross-linking agents. The resulting composite aerogel exhibits excellent adsorption performance for enrofloxacin, malachite green, Congo red, rhodamine, and kanamycin in water, with adsorption equilibrium times all within 10 minutes, enabling rapid removal of organic pollutants from water. This invention provides a new approach for the high-value utilization of Eucommia ulmoides gum and has promising application prospects in the field of water environment remediation.

This invention discloses a G4-DNA tetrahedral probe electrode, its preparation method, applications, and application methods. This invention utilizes four DNA strands to self-assemble into a DNA tetrahedral structure based on Watson-Crick hybridization. The DNA tetrahedron and G4 strand can be connected to the electrode based on the interaction between thiol groups and the gold electrode, and complementary base pairing. The addition of the DNA tetrahedron, through the construction of a reasonable probe density, further improves the detection sensitivity. This invention utilizes square wave voltammetry for electrochemical detection of malachite green. The content of malachite green can be determined based on the strength of the current signal of its characteristic peak. Rapid detection of malachite green can be achieved in a short time, with a minimum detection concentration of 3.65 μg / kg, showing potential for on-site detection. Six repeated tests using this electrode yielded almost identical detection signals, indicating that the electrode has good reusability and stability.

The present application relates to the technical field of water treatment, in particular to a kind of lemon acid modified bagasse biochar and its preparation method and application in removing malachite green in water.The present application uses bagasse as raw material, uses lemon acid as modifier, prepares the alkali treated lemon acid modified bagasse biochar by high-temperature limited oxygen pyrolysis, and is used to absorb organic dye malachite green in water, and the adsorption capacity can reach 1139 mg·g ‑1 .

The invention discloses a nucleic acid aptamer with synergistically enhanced fluorescence property and affinity, and application of the nucleic acid aptamer in a malachite green label-free proportional biosensor. Specifically, the malachite green aptamer biosensor with excellent detection performance is constructed through a competitive balance principle, and the malachite green aptamer biosensor has a wide detection range and a low detection limit. The nucleic acid aptamer and the biosensor provided by the invention can assist the development of a malachite green detection method and the development of the field of malachite green food safety or environment detection kits, and have certain universality and industrialization potential.

The invention provides a rare earth metal-organic framework material as well as a preparation method and application thereof, and relates to the technical field of fluorescence detection, the general formula of the material is {[Ln4 (THBA) 2 (H2O) 5]. 4H2O} n, Ln is Eu < 3 + > or Tb < 3 + > ions; tHBA is a deprotonated [1, 1 ', 4', 1 ''-terphenyl]-2 ', 3, 3', 5, 5 ', 5''-hexacarboxylic acid ligand; the material belongs to a triclinic system, the space group is P1, the material has a three-dimensional porous frame structure, and each Ln < 3 + > ion is in an eight-coordinate configuration; n > = 1, and n is an integer. The material not only shows high-sensitivity, high-selectivity and quick-response detectability to the harmful substance malachite green, but also successfully realizes conversion from a powder material to a portable device, and has wide application prospects in the fields of environmental monitoring, food safety, information encryption and the like.

The invention belongs to the technical field of fluorescence detection, and particularly relates to a ratio-dependent up-conversion fluorescence nanoprobe for veterinary drug detection and a preparation method and application of the ratio-dependent up-conversion fluorescence nanoprobe. The invention provides a ratio type up-conversion fluorescent nanoprobe for veterinary drug detection. The ratio type up-conversion fluorescent nanoprobe comprises a core nanoparticle NaGdF4: Yb / Tm, an inner layer shell NaYbF4, a middle layer shell NaGdF4: Yb / Er and an outer layer shell NaYF4 from inside to outside, after the fluorescent probe is incubated with furaltadone and malachite green, the fluorescence intensity is quenched, the selectivity is good, and the fluorescent probe can be used for detecting furaltadone and malachite green; furthermore, as a ratio-type up-conversion fluorescent nanoprobe, the fluorescent nanoprobe can be excited by low-energy near-infrared light, does not cause light damage, has higher biological tissue penetration depth, no background fluorescence interference and higher signal-to-noise ratio, and is a fluorescence detection method for veterinary drug residues with excellent reliability; therefore, the technical problem of low reliability of fluorescence detection of veterinary drug residues in the prior art is solved.

The invention discloses a method for rapidly detecting malachite green in aquatic products, and belongs to the technical field of biological detection. According to the time-resolved fluorescent microsphere immunochromatography kit based on the specific nano-antibody, the nano-antibody is applied to immunochromatography, an optimized pretreatment method is combined, malachite green in aquatic products can be rapidly detected on site, sample pretreatment and detection can be completed within 15 min, the limit of detection (LOQ) is 0.03 mu g / kg, and the time-resolved fluorescent microsphere immunochromatography kit can be used for detecting malachite green in aquatic products. The naked eye line elimination value is 1.6 mu g / kg which is far higher than the detection level in the prior art, and the method has the advantages of high sensitivity, good specificity, simplicity in operation, short detection time and the like, and is suitable for on-site rapid screening of malachite green residues in aquatic products.

Preparation of nitrogen-sulfur co-doped canna flower-based biochar and a use thereof in the removal of malachite green in wastewater. The method comprises: washing, drying, and pulverizing a collected canna flower, mixing same with a certain amount of phosphoric acid in a beaker, adding an appropriate amount of deionized water, stirring same, loading a feed liquid into a hydrothermal kettle, and then placing same in an oven for hydrothermal treatment to obtain a canna flower-based hydrothermal carbon; uniformly mixing hydrothermal carbon powder with a certain mass ratio of thiourea and molten salt, placing the mixture in a muffle furnace for firing in air, cooling same with the furnace after firing is completed, finally taking out a sample, separating the molten salt, performing acid washing on the biochar, washing same with alcohol, washing same with water, and then drying same to obtain the nitrogen-sulfur co-doped canna flower-based biochar; and using the nitrogen-sulfur co-doped canna flower-based biochar to remove malachite green in wastewater. Compared with conventional carbon materials, the nitrogen-sulfur co-doped carbon material prepared by the method has greatly improved removal efficiency for malachite green in a water body. In addition, the preparation method provides a way to treat a biomass waste, saves costs, and realizes resource utilization of biomass wastes.

This invention belongs to the field of environmental pollution detection and treatment technology, specifically relating to a SiO2@Ag / ZnO flexible substrate and its application in the detection and degradation of malachite green. A SiO2@Ag nanocomposite material is prepared by modifying Ag nanoparticles onto the surface of SiO2 spheres. Further, ZnO is attached to the surface of SiO2@Ag to prepare the composite material SiO2@Ag / ZnO. A SiO2@Ag / ZnO flexible substrate was successfully prepared by attaching SiO2@Ag / ZnO to fabric. This material exhibits detection capability at lower concentrations and demonstrates stable response to concentration changes in SERS signal detection of malachite green, exhibiting high quantitative accuracy and suitability for SERS quantitative analysis. Simultaneously, this material achieves stable degradation of malachite green under light irradiation conditions; the degradation rate gradually increases with prolonged ultraviolet light irradiation time, reaching a maximum degradation rate of 91.1%.

The present application relates to the technical field of immunochromatographic analysis, and discloses a signal enhancer for a colloidal gold immunochromatographic rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, signal amplification is realized by catalytic deposition of silver nanoparticles on the surface of colloidal gold, the present application is simple to operate, is added at one time, and reaction is completed in 5-10 minutes; sensitivity is improved by 5-10 times, specificity is higher than 98%; stability is excellent, 12 months of storage at 4 DEG C; it is compatible with various colloidal gold rapid detection cards; cost is controllable, single detection is increased by 0.3-0.5 yuan, and the present application is especially suitable for super-sensitive detection of small molecule substances in the field of food safety, such as detection limit of malachite green of 0.1 microgram per liter, detection limit of fipronil of 0.5 microgram per liter, and the application range of the colloidal gold immunochromatographic rapid detection card is significantly expanded.

The application discloses a double-emission ratio fluorescent probe RB@Zr-fcu-sti, a preparation method thereof and application thereof in detection of malachite green (MG). The preparation method comprises the following steps: adding Zr-fcu-sti and rhodamine B into anhydrous ethanol, ultrasonic dispersion, stirring reaction at room temperature in dark, centrifugation, washing, and vacuum drying. The double-emission ratio fluorescent probe RB@Zr-fcu-sti is applied to the fluorescence detection of malachite green, and has high sensitivity and fast response. With the increase of the concentration of the sensing system malachite green (MG), the red fluorescence emission of the probe is significantly quenched, while the intensity of the blue fluorescence emission remains basically unchanged, so that the fluorescence color of the sensing system gradually changes from purple to blue. The detection limit of the application to malachite green (MG) is as low as 2.69nM, and thus the application can be applied to high-sensitivity ratio fluorescence sensing of malachite green (MG).

The invention discloses a visible / ultraviolet dual-light synergistically enhanced substrate, a preparation method thereof and application of the visible / ultraviolet dual-light synergistically enhanced substrate in rapid detection of veterinary drugs. According to the method, a gas-liquid interface self-assembly technology is combined with a light welding process, and the heterojunction composite Raman substrate Bi: ZnO-SUSNs of a Bi-doped zinc oxide nano array and urchin-shaped silver nanoparticles is prepared. The Bi: ZnO-SUSNs composite Raman substrate disclosed by the invention has excellent visible light / ultraviolet light dual response characteristics, and the detection sensitivity to target molecules can be greatly improved under dual-light driving by utilizing a PIERS mechanism. According to the method, trace detection of veterinary drugs such as malachite green, crystal violet and furacilin can be realized without complex pretreatment. In addition, the composite substrate also has a photocatalytic degradation function, can realize self-cleaning and reutilization, and has the advantages of low preparation cost, high detection speed, good repeatability and the like.

The invention discloses an application of a malachite green aptamer in low-damage slow-release bacteriostasis and long-acting bacteriostasis. Specifically, the nucleic acid aptamer with improved affinity, luminescence property and stability can slowly release malachite green in the bacteriostasis process, and assists the long-acting and mild bacteriostasis process. The aptamer with improved multi-dimensional performance is applied to the field of slow-release bacteriostasis, can effectively relieve toxic and side effects of a bactericide on non-target microorganisms or host cells, and is beneficial to realizing long-acting control and relieving drug resistance.

The present application relates to the technical field of food quality supervision, and particularly relates to a method for rapidly detecting sulfur dioxide in shrimps based on fading technology of addition reaction. The method comprises the following steps: S1, weighing the sample to be measured, adding pure water for homogenization, centrifuging, and obtaining supernatant, which is used as sample extract; S2, taking a test tube, adding V1 volume of sample extract and V2 volume of acetonitrile, shaking, centrifuging, making the upper liquid clear, and taking the upper liquid as positive reference; V2 / (V1+V2)=40-70%; taking another test tube for synchronous operation to make the upper liquid clear, then adding malachite green reagent dropwise, shaking gently, and standing for 4-10 min; comparing the test tube with the positive reference, and judging the content of sulfur dioxide in the sample to be measured according to the fading of the lake blue in the test tube. The present application uses dynamic fading reaction to replace static colorimetric card colorimetry, and avoids the subjectivity and matrix interference problems of color card method, and is especially suitable for rapid detection of shrimp samples.

This invention discloses a method for detecting malachite green residues in food based on a ratiometric fluorescent paper-based sensor using titanium carbide quantum dots and biomass carbon quantum dots. First, titanium carbide quantum dots and biomass carbon quantum dots are prepared. Then, a Ti3C2MXene / biomass quantum dot ratiometric fluorescent probe is prepared. Based on this, a Ti3C2MXene / biomass quantum dot ratiometric fluorescent paper-based sensor (filter paper chip) is constructed. A linear equation for the working curve of malachite green concentration is established. Finally, the pretreated sample solution is dropped onto the ratiometric fluorescent filter paper chip. After drying, the paper chip is photographed using a mobile phone under a 365 nm UV analyzer. The RGB values ​​of the paper chip are extracted using smartphone color recognition software. The obtained R value is substituted into the linear equation of the working curve to obtain the malachite green concentration in the sample. The titanium carbide quantum dot / biomass carbon quantum dot ratiometric fluorescent paper-based sensor and malachite green concentration detection method disclosed in this invention are sensitive, accurate, and rapid.

The invention discloses a copper oxide loaded sea stone composite material for water treatment and a preparation method thereof, sepiolite is loaded with copper oxide to obtain composite particles, and the composite material composed of a plurality of composite particles can effectively adsorb malachite green and other cationic dye molecules and has higher cyclic regeneration performance. The CuO active component on the surface of the composite material has good antibacterial performance, and can synergistically realize water purification and bacteriostasis functions.

The application discloses a SERS substrate for detecting malachite green and a preparation method thereof, and belongs to the technical field of Raman spectroscopy.The preparation method of the substrate is as follows: a PET film is preprocessed by sanding to form a micron-level gully structure on the surface of the PET film; the treated PET film is subjected to oxygen plasma treatment to etch nano-protrusions on the surface of the micron-level gully structure and introduce oxygen-containing polar groups on the surface of the PET film; and a silver layer is grown on the treated PET film by a vacuum evaporation method to obtain a SERS substrate with a micro-nano composite structure for detecting malachite green.The method constructs a micro-nano composite structure on the surface of the PET film through the synergistic effect of sanding and oxygen plasma treatment, greatly improves the SERS activity of the subsequently deposited silver layer, significantly enhances the bonding force between the silver layer and the substrate, and enables the substrate to have high sensitivity, high uniformity, excellent mechanical stability and reusability.

This invention relates to a wastewater treatment method based on carbon materials used in methane cracking for hydrogen production. The method involves cracking methane to produce hydrogen using an iron-based catalyst, collecting the carbon material byproducts, and utilizing the adsorption properties of carbon nanotubes in the byproducts and the catalytic properties of residual metals (such as iron) in the catalyst, combined with hydroxyl radicals (·OH) generated by the Fenton reaction, to achieve efficient removal of organic pollutants (such as dyes methylene blue and malachite green) from the wastewater. An external magnetic field can be applied to rapidly recover the carbon materials, enabling resource recycling.

The application discloses a multi-dimensional performance-improved aptamer, and applies the aptamer to a malachite green label-free proportional biosensor and long-acting, mild and slow-release bacteriostatic regulation. Specifically, two kinds of malachite green aptamer biosensors with superior detection performance are constructed through a competition balance principle, and the two kinds of biosensors have a wide detection range and a low detection limit. In addition, the aptamer can slow release malachite green in the bacteriostatic process, and help to realize long-acting and mild bacteriostatic process. Application of the multi-dimensional performance-improved aptamer to the slow-release bacteriostatic field can effectively alleviate the toxic side effects of bactericides on non-target microorganisms or host cells, and is beneficial to realize long-acting control and reduce drug resistance.

A preparation method of a gellan gum / ZIF-8 composite aerogel for dye adsorption, comprising the following steps: S1: preparing ZIF-8; S2: adding ZIF-8 into deionized water to obtain a ZIF-8 suspension A; S3: adding gellan gum into a 0.1 M potassium chloride solution, heating and stirring at 95 DEG C until completely dissolved to obtain a solution B; S4: adding the suspension A into the solution B, heating and stirring at 95 DEG C for 1.5 hours to obtain a mixed solution C; S5: pouring the mixed solution C into a corresponding mold to obtain a gellan gum / ZIF-8 composite hydrogel; S6: pre-freezing the gellan gum / ZIF-8 composite hydrogel at-18 DEG C for 12 hours, and then freeze-drying for 24 hours to obtain a gellan gum / ZIF-8 composite aerogel. The gellan gum / ZIF-8 composite aerogel has excellent adsorption effect on cationic dye malachite green.

This invention discloses a method for preparing manganese-loaded soybean residue baked charcoal and its application in purifying malachite green in ornamental fish aquaculture water. The invention uses soybean residue as raw material, which is washed, dried, and pulverized. It is then pretreated by soaking in a phosphoric acid solution, followed by manganese loading through soaking in a potassium permanganate buffer solution. Finally, the manganese-loaded soybean residue baked charcoal is obtained through low-temperature baking technology. This invention not only realizes the value-added utilization of soybean residue and low-temperature waste heat resources, but also the prepared manganese-loaded soybean residue baked charcoal exhibits a high adsorption capacity for malachite green in water, with a maximum adsorption capacity of 373.4 mg / g, making it a highly efficient adsorbent for purifying malachite green in ornamental fish aquaculture water.

The application belongs to the field of fluorescent sensors, and discloses a dual-emission fluorescent sensor for malachite green detection and removal, and a preparation method and application thereof. 3+ By introducing 2-quinoxaline carboxylic acid into UiO-66-(COOH)2, and then modifying and doping Eu 0000001 , the Eu-MOF luminescent material EuUQCA is constructed. The material can be used as a fluorescent sensor to quickly and sensitively detect MG, and can realize quantitative detection of MG in complex fishpond water and fish samples, and good recovery is obtained. More importantly, the EuUQCA powder can effectively adsorb and remove MG from fishpond water. The material can realize recycling as a fluorescent sensor and an adsorbent, and provides an effective method for constructing a sensing and adsorbing material which can sensitively detect MG and effectively remove MG residues.

This invention discloses a three-dimensional cobalt metal-organic complex, its preparation method, and its applications, belonging to the field of crystal materials technology. This invention uses 2,5-diketopiperazine-N,N'-diacetic acid and 4,4'-bipyridine as raw materials with cobalt ions to prepare a compound with the chemical formula [Co(DPDA)(4,4'-bpy)]. n A three-dimensional cobalt-organic metal complex. It has a six-connected three-dimensional network structure with dual-core [Co2(COO)2] structural units, and its topological symbol is {3 12 0.4 24 0.5 9 Furthermore, the aforementioned three-dimensional cobalt metal-organic complex can rapidly activate peroxymonosulfate to effectively degrade dyes. It can degrade methylene blue, malachite green, and crystal violet to over 95.0% within 5 minutes, without producing toxic byproducts during the degradation process, and exhibits good reusability. Additionally, the synthesis conditions of the three-dimensional cobalt metal-organic complex are mild, the steps are simple, and it possesses excellent structural stability, good thermal stability, and antiferromagnetism at high temperatures.

This invention discloses a high-performance laccase mutant constructed based on multi-source gene recombination and its applications. Using BspLac from Bacillus sp. SL-1 and BLTCLac from Bacillus licheniformis TCCC111219 as templates, a mutant library DS was constructed using DNA shuffling technology. Two high-performance laccase mutants, DS2 and DS5, were obtained through screening. Dynamic enzyme production analysis showed that the laccase activity of DS2 peaked at 1922 U / L after 24 hours, equivalent to twice that of the original strain; the laccase activity of DS5 peaked at 1337 U / L after 24 hours, equivalent to 1.4 times that of the original strain. Simultaneously, experimental verification showed that DS2 and DS5 achieved degradation rates of 83% and 89% for malachite green, respectively. This laccase mutant significantly improved the detoxification efficiency of synthetic dyes and possesses good potential for industrial application.

The application discloses a preparation method of an adsorbent for removing malachite green in wastewater, and comprises the following steps: collecting poplar catkins around a campus, removing other sundries, washing and flushing the poplar catkins with tap water, drying the poplar catkins, soaking the poplar catkins in a solution containing 3-chloro-2-hydroxypropane sulfonic acid sodium, and performing ultrasonic treatment to obtain hydrophilic poplar catkins; after the hydrophilic poplar catkins are squeezed dry, the poplar catkins are placed in a mixed solution of boric acid-triethylamine, modified treatment is performed under the action of ultrasonic waves, the poplar catkins are taken out after a period of reaction, are filtered, are washed with a dilute acetic acid solution for several times, are washed with distilled water until the pH is 5-7, and are dried in an oven to obtain fibrous poplar catkin adsorbents, which are used for removing malachite green and other cationic dyes in wastewater; the adsorbent preparation method is simple, has large adsorption capacity, and is a relatively good adsorbent for removing malachite green in wastewater.

The present application relates to the technical field of supramolecular chemistry, and particularly relates to a pyrimidine acylhydrazine organic gel factor and preparation and application thereof.The preparation process of the pyrimidine acylhydrazine organic gel factor is simple, and the organic gel prepared by further using the pyrimidine acylhydrazine derivative organic gel factor can effectively adsorb and remove bright green, malachite green, crystal violet, rhodamine B and other organic dyes.Among them, the removal rate of bright green dye can be as high as 99.86%, and the removal efficiency of rhodamine B dye can be as high as 99.89%;it is shown that the organic gel of the present application has excellent removal efficiency on organic dyes.

The application provides a rare earth metal-organic framework material, a preparation method and application thereof, relates to the field of fluorescent detection technology, and the general formula of the material is {[Ln4(THBA)2(H2O)5]·4H2O} n , wherein Ln is Eu 3+ , or Tb 3+ ; THBA is a deprotonated [1,1',4',1''-triphenyl] 2',3,3',5,5',5''-hexacarboxylic acid ligand; the material belongs to a triclinic system, the space group is P 1, has a three-dimensional porous framework structure, and each Ln 3+ ion is in an octahedral coordination; n is greater than or equal to 1, and n is an integer. The material not only shows high sensitivity, high selectivity and rapid response detection capability for harmful substances malachite green, but also successfully realizes conversion from a powder material to a portable device, and has wide application prospects in the fields of environmental monitoring, food safety and information encryption.

The present application relates to the technical field of food quality detection, and particularly relates to a fast detection method and kit for sulfur dioxide in starch products based on colloidal gold immunochromatography technology. The fast detection method comprises the following steps: preparing a detection solution: quantitatively weighing the starch product, extracting sulfur dioxide at normal temperature or low temperature with a caustic solution, and centrifuging the extraction solution to obtain the upper clear liquid. Intermediate reaction: measuring the detection solution, calculating the limited molar amount of sulfur dioxide according to the limited value and extraction rate of sulfur dioxide, adding an equal molar amount of DDQ reagent and LMG reagent, and reacting at pH 6.5-7.5. Colloidal gold reaction: adding the reaction solution dropwise to a malachite green colloidal gold immunochromatography detection card. Judgment: if the redness of the T line is deeper than that of the C line or the same, the malachite green is negative, and the sulfur dioxide is over-limited; if the redness of the T line is lighter or not colored, the malachite green is positive, and the sulfur dioxide is not over-limited; and if the C line is not colored, the detection is invalid. The present application is suitable for rapid qualitative detection of sulfur dioxide in starch.

The invention discloses a three-phase interface self-assembly gold nano triangular single-layer film array SERS (Surface Enhanced Raman Scattering) substrate based on PVP (Polyvinyl Pyrrolidone) concentration and halide ion dual optimization and application thereof, and aims to solve the problems of poor uniformity, poor reproducibility and insufficient multi-target detection capability of the existing SERS substrate. According to the technical scheme, the method comprises the steps that AuNTs colloid is prepared and purified, ligands are optimized through a 0.001%-0.1% PVP-ethanol solution, an AuNTs single-layer film is formed through three-phase interface self-assembly, and then a 5-40 mM halogen ion solution is used for treating and reducing array gaps; and the optimal condition is 0.01% PVP (Polyvinyl Pyrrolidone) and 20mM NaCl. The substrate can realize high-sensitivity detection on malachite green (detection limit of 0.05 mu g / L) in aquatic products, pirimicarb (detection limit of 10 mu g / L) in vegetables and beta-carotene (detection limit of 0.05 mu g / mL) in apricots, the relative standard deviation is less than or equal to 6.75%, multi-target simultaneous detection can be realized, and the substrate is suitable for rapid quantitative analysis of food safety.