ELISA reagent for detecting malachite green and method
An enzyme-linked immunosorbent reagent, malachite green technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming detection, expensive sample processing, complexity, etc., and achieve simple operation, simple sample pretreatment, and strong specificity Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] Example 1 Preparation of ELISA Kit Components for Malachite Green Detection
[0048] 1. Antigen Synthesis
[0049] a. Synthesis of Coating Progen
[0050] The malachite green hapten is synthesized by the derivative method, and then the hapten is obtained by coupling the hapten with the bovine gamma globulin carrier protein by the active ester method through the diazotization reaction.
[0051] b. Synthesis of Immunogen
[0052] The malachite green hapten is obtained by coupling the malachite green hapten with the keyhole limpet hemocyanin carrier protein by the active ester method through diazotization reaction.
[0053] 2. Preparation of Malachite Green Mouse Monoclonal Antibody
[0054] a. Animal immunity
[0055] Balb / c mice were used as immunized animals, and the conjugated malachite green hapten and keyhole limpet hemocyanin was used as the immunogen, and the immunization dose was 300 μg / mouse. The adjuvant was mixed to make an emulsifier, which was injected s...
Embodiment 2
[0070] Example 2 Detecting the formation of malachite green monoclonal ELISA kit
[0071] Set up the enzyme-linked immunosorbent assay kit for detection of malachite green, so that it contains the following components:
[0072] (1) enzyme-linked plate coated with malachite green antigen;
[0073] (2) Goat anti-mouse anti-antibody labeled with horseradish peroxidase;
[0074] (3) malachite green mouse monoclonal antibody;
[0075] (4) 6 bottles of malachite green standard solution, the concentrations are 0μg / L, 0.01μg / L, 0.05μg / L, 0.1μg / L, 0.5μg / L, 1μg / L;
[0076] (5) The substrate chromogenic solution is a mixed solution of hydrogen peroxide or carbamide peroxide and o-phenylenediamine (OPD) or tetramethylbenzidine (TMB);
[0077] (6) The stop solution is 2mol / L sulfuric acid buffer;
[0078] (7) Concentrated washing solution: phosphate buffer containing 0.1% to 0.5% Tween 80 and 0.5% sodium azide;
[0079] (8) Antibody diluent is pH 8.2, 0.05 mol / L, phosphate buffer cont...
Embodiment 3
[0081] Example 3 Detecting the formation of malachite green polyclonal ELISA kit
[0082] Set up the enzyme-linked immunosorbent assay kit for detection of malachite green, so that it contains the following components:
[0083] (1) Enzyme-linked plate coated with goat anti-rabbit anti-antibody;
[0084] (2) malachite green antigen labeled with alkaline phosphatase;
[0085] (3) Malachite green rabbit polyclonal antibody;
[0086] (4) 6 bottles of malachite green standard solution, the concentrations are 0μg / L, 0.01μg / L, 0.05μg / L, 0.1μg / L, 0.5μg / L, 1μg / L;
[0087] (5) Substrate chromogenic solution p-nitrophosphate buffer;
[0088] (6) The stop solution is 2mol / L sodium hydroxide buffer solution;
[0089] (7) Concentrated washing solution containing 0.1% to 0.5% Tween 80, 0.5% sodium azide in phosphate buffer, 1 bottle;
[0090] (8) The concentrated complex solution is a phosphate buffer solution containing 20% methanol and 1% bovine serum (BSA).
PUM
Property | Measurement | Unit |
---|---|---|
absorbance | aaaaa | aaaaa |
absorbance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com