11 results about "Malachite green stain" patented technology

This invention discloses a G4-DNA tetrahedral probe electrode, its preparation method, applications, and application methods. This invention utilizes four DNA strands to self-assemble into a DNA tetrahedral structure based on Watson-Crick hybridization. The DNA tetrahedron and G4 strand can be connected to the electrode based on the interaction between thiol groups and the gold electrode, and complementary base pairing. The addition of the DNA tetrahedron, through the construction of a reasonable probe density, further improves the detection sensitivity. This invention utilizes square wave voltammetry for electrochemical detection of malachite green. The content of malachite green can be determined based on the strength of the current signal of its characteristic peak. Rapid detection of malachite green can be achieved in a short time, with a minimum detection concentration of 3.65 μg / kg, showing potential for on-site detection. Six repeated tests using this electrode yielded almost identical detection signals, indicating that the electrode has good reusability and stability.

The present application relates to the technical field of water treatment, in particular to a kind of lemon acid modified bagasse biochar and its preparation method and application in removing malachite green in water.The present application uses bagasse as raw material, uses lemon acid as modifier, prepares the alkali treated lemon acid modified bagasse biochar by high-temperature limited oxygen pyrolysis, and is used to absorb organic dye malachite green in water, and the adsorption capacity can reach 1139 mg·g ‑1 .

This invention belongs to the field of environmental pollution detection and treatment technology, specifically relating to a SiO2@Ag / ZnO flexible substrate and its application in the detection and degradation of malachite green. A SiO2@Ag nanocomposite material is prepared by modifying Ag nanoparticles onto the surface of SiO2 spheres. Further, ZnO is attached to the surface of SiO2@Ag to prepare the composite material SiO2@Ag / ZnO. A SiO2@Ag / ZnO flexible substrate was successfully prepared by attaching SiO2@Ag / ZnO to fabric. This material exhibits detection capability at lower concentrations and demonstrates stable response to concentration changes in SERS signal detection of malachite green, exhibiting high quantitative accuracy and suitability for SERS quantitative analysis. Simultaneously, this material achieves stable degradation of malachite green under light irradiation conditions; the degradation rate gradually increases with prolonged ultraviolet light irradiation time, reaching a maximum degradation rate of 91.1%.

This invention discloses a method for detecting malachite green residues in food based on a ratiometric fluorescent paper-based sensor using titanium carbide quantum dots and biomass carbon quantum dots. First, titanium carbide quantum dots and biomass carbon quantum dots are prepared. Then, a Ti3C2MXene / biomass quantum dot ratiometric fluorescent probe is prepared. Based on this, a Ti3C2MXene / biomass quantum dot ratiometric fluorescent paper-based sensor (filter paper chip) is constructed. A linear equation for the working curve of malachite green concentration is established. Finally, the pretreated sample solution is dropped onto the ratiometric fluorescent filter paper chip. After drying, the paper chip is photographed using a mobile phone under a 365 nm UV analyzer. The RGB values ​​of the paper chip are extracted using smartphone color recognition software. The obtained R value is substituted into the linear equation of the working curve to obtain the malachite green concentration in the sample. The titanium carbide quantum dot / biomass carbon quantum dot ratiometric fluorescent paper-based sensor and malachite green concentration detection method disclosed in this invention are sensitive, accurate, and rapid.

The application discloses a SERS substrate for detecting malachite green and a preparation method thereof, and belongs to the technical field of Raman spectroscopy.The preparation method of the substrate is as follows: a PET film is preprocessed by sanding to form a micron-level gully structure on the surface of the PET film; the treated PET film is subjected to oxygen plasma treatment to etch nano-protrusions on the surface of the micron-level gully structure and introduce oxygen-containing polar groups on the surface of the PET film; and a silver layer is grown on the treated PET film by a vacuum evaporation method to obtain a SERS substrate with a micro-nano composite structure for detecting malachite green.The method constructs a micro-nano composite structure on the surface of the PET film through the synergistic effect of sanding and oxygen plasma treatment, greatly improves the SERS activity of the subsequently deposited silver layer, significantly enhances the bonding force between the silver layer and the substrate, and enables the substrate to have high sensitivity, high uniformity, excellent mechanical stability and reusability.

A preparation method of a gellan gum / ZIF-8 composite aerogel for dye adsorption, comprising the following steps: S1: preparing ZIF-8; S2: adding ZIF-8 into deionized water to obtain a ZIF-8 suspension A; S3: adding gellan gum into a 0.1 M potassium chloride solution, heating and stirring at 95 DEG C until completely dissolved to obtain a solution B; S4: adding the suspension A into the solution B, heating and stirring at 95 DEG C for 1.5 hours to obtain a mixed solution C; S5: pouring the mixed solution C into a corresponding mold to obtain a gellan gum / ZIF-8 composite hydrogel; S6: pre-freezing the gellan gum / ZIF-8 composite hydrogel at-18 DEG C for 12 hours, and then freeze-drying for 24 hours to obtain a gellan gum / ZIF-8 composite aerogel. The gellan gum / ZIF-8 composite aerogel has excellent adsorption effect on cationic dye malachite green.

This invention discloses a three-dimensional cobalt metal-organic complex, its preparation method, and its applications, belonging to the field of crystal materials technology. This invention uses 2,5-diketopiperazine-N,N'-diacetic acid and 4,4'-bipyridine as raw materials with cobalt ions to prepare a compound with the chemical formula [Co(DPDA)(4,4'-bpy)]. n A three-dimensional cobalt-organic metal complex. It has a six-connected three-dimensional network structure with dual-core [Co2(COO)2] structural units, and its topological symbol is {3 12 0.4 24 0.5 9 Furthermore, the aforementioned three-dimensional cobalt metal-organic complex can rapidly activate peroxymonosulfate to effectively degrade dyes. It can degrade methylene blue, malachite green, and crystal violet to over 95.0% within 5 minutes, without producing toxic byproducts during the degradation process, and exhibits good reusability. Additionally, the synthesis conditions of the three-dimensional cobalt metal-organic complex are mild, the steps are simple, and it possesses excellent structural stability, good thermal stability, and antiferromagnetism at high temperatures.

This invention discloses a high-performance laccase mutant constructed based on multi-source gene recombination and its applications. Using BspLac from Bacillus sp. SL-1 and BLTCLac from Bacillus licheniformis TCCC111219 as templates, a mutant library DS was constructed using DNA shuffling technology. Two high-performance laccase mutants, DS2 and DS5, were obtained through screening. Dynamic enzyme production analysis showed that the laccase activity of DS2 peaked at 1922 U / L after 24 hours, equivalent to twice that of the original strain; the laccase activity of DS5 peaked at 1337 U / L after 24 hours, equivalent to 1.4 times that of the original strain. Simultaneously, experimental verification showed that DS2 and DS5 achieved degradation rates of 83% and 89% for malachite green, respectively. This laccase mutant significantly improved the detoxification efficiency of synthetic dyes and possesses good potential for industrial application.

The application belongs to the technical field of SERS detection, and particularly relates to an Ag-CuCo 0.5 Fe 1.5 O4 composite SERS substrate for detecting malachite green in wastewater. CuCo 0.5 Fe 1.5 O4 nanomaterials are synthesized by a calcination method, AgNPs are in-situ loaded on the surface of the CuCo 0.5 Fe 1.5 O4 composite SERS substrate is constructed, and the substrate is fixed on the surface of a glass sheet. The substrate fully combines the local surface plasmon resonance effect of AgNPs and the cation rearrangement and lattice distortion characteristics generated by CuCo 0.5 Fe 1.5 O4, effectively promotes the separation and transmission of photo-generated carriers. The Co / Fe synergistic effect endows the material with rich defect sites and controllable electronic structure, is conducive to forming an efficient interface charge transfer path, thereby significantly enhancing the Raman signal and realizing sensitive detection of trace malachite green in wastewater.