79 results about "Malachite green stain" patented technology

The application discloses a malachite green label-free proportional biosensor based on multi-dimensional cutting aptamer, which comprises the following steps: (1) a performance-improved malachite green aptamer sequence; (2) a label-free proportional malachite green sensor sequence; and (3) malachite green detection. The biosensor is constructed by using various aptamer sequences, and the ideal sensing effect can be realized through the competition balance principle. The label-free proportional sensor can present a good linear relationship in the range of 5nM-4muM, and has a low detection limit of nanomolar level, which indicates that the sensor has good malachite green detection potential and can meet the application requirements.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The present invention relates to a method and a dual-color detection kit for the rapid and simultaneous detection of MS-222 and malachite green, and a technology for the rapid detection of veterinary drug residues in aquatic products. The invention utilizes red and blue colloidal gold with different particle sizes, combined with specific antibodies to construct different red and blue probes, respectively. The invention utilizes the principle that MG-BSA complete antigen and 3-aminobenzoic acid-BSA antigen coated on the T1 line and the T2 line, respectively, can compete with MG and MS-222 in the sample for binding to the blue probe and the red probe, thereby constructing a dual-color rapid detection test strip for MS-222 and malachite green that is clearly distinguishable to the naked eye and simple to operate.

The invention discloses an aptamer with improved multi-dimensional performance. The aptamer is applied to a malachite green label-free proportional biosensor and long-acting, mild and slow-release antibacterial regulation and control. Specifically, two malachite green aptamer biosensors with excellent detection performance are constructed through a competitive balance principle, and the malachite green aptamer biosensors have a wide detection range and a low detection limit. In addition, the aptamer can slowly release malachite green in the bacteriostasis process, and the long-acting and mild bacteriostasis process is assisted. The aptamer with improved multi-dimensional performance is applied to the field of slow-release bacteriostasis, can effectively relieve toxic and side effects of a bactericide on non-target microorganisms or host cells, and is beneficial to realizing long-acting control and relieving drug resistance.

This invention discloses a G4-DNA tetrahedral probe electrode, its preparation method, applications, and application methods. This invention utilizes four DNA strands to self-assemble into a DNA tetrahedral structure based on Watson-Crick hybridization. The DNA tetrahedron and G4 strand can be connected to the electrode based on the interaction between thiol groups and the gold electrode, and complementary base pairing. The addition of the DNA tetrahedron, through the construction of a reasonable probe density, further improves the detection sensitivity. This invention utilizes square wave voltammetry for electrochemical detection of malachite green. The content of malachite green can be determined based on the strength of the current signal of its characteristic peak. Rapid detection of malachite green can be achieved in a short time, with a minimum detection concentration of 3.65 μg / kg, showing potential for on-site detection. Six repeated tests using this electrode yielded almost identical detection signals, indicating that the electrode has good reusability and stability.

This invention discloses a molecularly switched adsorbent for adsorbing triphenylmethane dyes and its application, belonging to the field of wastewater treatment technology. The molecularly switched adsorbent proposed in this invention can rapidly remove triphenylmethane dyes such as crystal violet, malachite green, and methylene blue dyes from water; it can remove tens of ppm concentrations of crystal violet, malachite green, and methylene blue dyes from water in a very short time (90 min), with a removal rate of ≥92%. Moreover, this adsorbent can maintain a removal rate of ≥90% under a wide pH range (6-11) and high ion concentration conditions (1M). The activation and regeneration conditions of the adsorbent of this invention are mild; dye molecules can be removed by elution in a low-concentration alkaline solution only a few times. The adsorbent can be continuously recycled at least 10 times without significant attenuation of adsorption capacity.

The present invention discloses an application of a mixed bacterial fermentation broth in the decolorization of triphenylmethane dyes. The mixed bacterial fermentation broth comprises a mixture of fermentation broths of one or more of Bacillus subtilis, Pseudomonas or Actinomycetes. The mixed bacterial fermentation broth constructed in the present invention for the efficient decolorization of triphenylmethane dyes can decolorize more than 70% of water-soluble aniline blue under both static and shaking conditions, and can tolerate a pH of about 4-8 and a high concentration of water-soluble aniline blue. In addition, the bacterial fermentation broth also has a good decolorization effect on other triphenylmethane dyes. It can decolorize more than 90% of basic fuchsin and malachite green within 48 hours, and also has a certain decolorization effect on three triphenylmethane dyes, namely methyl violet, acid blue 1 and acid red 94. The types of triphenylmethane dyes decolorized are significantly more than those of the reported microbial strains, showing high broad-spectrum property, and having the possibility of being actually applied to solve triphenylmethane dye wastewater.

The invention relates to the technical field of water treatment, in particular to citric acid modified bagasse biochar, a preparation method of the citric acid modified bagasse biochar and application of the citric acid modified bagasse biochar in removal of malachite green in water. The alkali-treated citric acid modified bagasse biomass charcoal is prepared by taking bagasse as a raw material and citric acid as a modifier through high-temperature oxygen-limited pyrolysis, the alkali-treated citric acid modified bagasse biomass charcoal is used for adsorbing organic dye malachite green in water, and the adsorption capacity can reach 1139 mg.g <-1 >.

The present application relates to the technical field of water treatment, in particular to a kind of lemon acid modified bagasse biochar and its preparation method and application in removing malachite green in water.The present application uses bagasse as raw material, uses lemon acid as modifier, prepares the alkali treated lemon acid modified bagasse biochar by high-temperature limited oxygen pyrolysis, and is used to absorb organic dye malachite green in water, and the adsorption capacity can reach 1139 mg·g ‑1 .

The invention discloses a nucleic acid aptamer with synergistically enhanced fluorescence property and affinity, and application of the nucleic acid aptamer in a malachite green label-free proportional biosensor. Specifically, the malachite green aptamer biosensor with excellent detection performance is constructed through a competitive balance principle, and the malachite green aptamer biosensor has a wide detection range and a low detection limit. The nucleic acid aptamer and the biosensor provided by the invention can assist the development of a malachite green detection method and the development of the field of malachite green food safety or environment detection kits, and have certain universality and industrialization potential.

The invention relates to a cobalt-doped MgAl-LDH adsorbent and a preparation method and application thereof.The cobalt-doped MgAl-LDH adsorbent is prepared by enabling cobalt nitrate, aluminum nitrate and magnesium nitrate to react through a precipitation method, and the cobalt nitrate, the aluminum nitrate and the magnesium nitrate are fed according to the molar ratio of Co < 2 + > to Al < 3 + > to Mg < 2 + > being (0.1-0.5): 1: (2-4). The cobalt-doped MgAl-LDH adsorbent disclosed by the invention has the advantages of simple preparation method, low cost and structures such as specific surface area, pore volume and pore diameter suitable for adsorbing malachite green, so that the cobalt-doped MgAl-LDH adsorbent is good in malachite green adsorption effect and high in adsorption capacity, and has a wide application prospect in the technical field of water treatment.

The invention provides a rare earth metal-organic framework material as well as a preparation method and application thereof, and relates to the technical field of fluorescence detection, the general formula of the material is {[Ln4 (THBA) 2 (H2O) 5]. 4H2O} n, Ln is Eu < 3 + > or Tb < 3 + > ions; tHBA is a deprotonated [1, 1 ', 4', 1 ''-terphenyl]-2 ', 3, 3', 5, 5 ', 5''-hexacarboxylic acid ligand; the material belongs to a triclinic system, the space group is P1, the material has a three-dimensional porous frame structure, and each Ln < 3 + > ion is in an eight-coordinate configuration; n > = 1, and n is an integer. The material not only shows high-sensitivity, high-selectivity and quick-response detectability to the harmful substance malachite green, but also successfully realizes conversion from a powder material to a portable device, and has wide application prospects in the fields of environmental monitoring, food safety, information encryption and the like.

The application discloses a metal composite Fenton-like solid catalyst and preparation and application of the metal composite Fenton-like solid catalyst in removal of malachite green and Cr(VI) in wastewater, and belongs to the technical field of degradation and removal of cationic dyes and chromium by adsorption. 2+ -Silica. Compared with liquid chelating agents involved in the existing research, EDDS in the catalyst structure can provide six coordination atoms due to the structure of four carboxyl and diimino groups, and can form a stable metal complex with Co 2+ by virtue of the superior metal chelating characteristics of the hexadentate ligand EDDS. The metal composite solid catalyst prepared by grafting the spacer arm on the silica gel matrix can be separated by filtration and the like, and the subsequent regeneration operation can be performed by selecting a suitable eluent, so that the metal composite solid catalyst has the characteristics of easy separation, regeneration and reusability.

The present invention relates to the technical field of rapid detection of malachite green, and specifically relates to a fluorescence sensor for detecting malachite green compounds, a preparation method thereof, and an application thereof. The fluorescence sensor includes: thioflavin T for generating a fluorescence signal; a nucleic acid aptamer for specifically binding to a malachite green compound; wherein, thioflavin T and the nucleic acid aptamer non-specifically bind to form a G-quadruplex-thioflavin T conjugate, which is the fluorescence sensor. The fluorescence sensor does not require modification with nanomaterials. When it is used for detecting malachite green compounds, the detection of malachite green compounds can be achieved without any separation steps. It has the characteristics of simplicity, rapidity, and high sensitivity, and the detection limit is 5.4 ng / L.

The invention discloses a malachite green detection device based on Raman. The malachite green detection device comprises a high-precision distribution sample introduction module which is used for controlling automatic distribution of an enhancement reagent and a malachite green solution; the micro-fluidic mixing module is connected with the high-precision distribution sample introduction module and is used for uniformly mixing a water sample to be detected and an enhancement reagent required by detection to form a mixed sample solution and outputting the mixed sample solution to the data acquisition module; the data acquisition module emits laser to the mixed sample liquid, acquires a Raman signal generated by the mixed sample liquid under the irradiation of the laser, obtains Raman spectrum data after photoelectric conversion and primary processing, and sends the Raman spectrum data to the data processing and display module for analysis; and the data processing and display module is used for further processing the Raman spectrum data of the mixed solution, carrying out quantitative analysis on the content of malachite green in the malachite green solution through an algorithm, and displaying a detection result. According to the invention, real-time and rapid detection of malachite green in aquatic water is realized, and full automation of the detection process can be realized.

The invention belongs to the technical field of fluorescence detection, and particularly relates to a ratio-dependent up-conversion fluorescence nanoprobe for veterinary drug detection and a preparation method and application of the ratio-dependent up-conversion fluorescence nanoprobe. The invention provides a ratio type up-conversion fluorescent nanoprobe for veterinary drug detection. The ratio type up-conversion fluorescent nanoprobe comprises a core nanoparticle NaGdF4: Yb / Tm, an inner layer shell NaYbF4, a middle layer shell NaGdF4: Yb / Er and an outer layer shell NaYF4 from inside to outside, after the fluorescent probe is incubated with furaltadone and malachite green, the fluorescence intensity is quenched, the selectivity is good, and the fluorescent probe can be used for detecting furaltadone and malachite green; furthermore, as a ratio-type up-conversion fluorescent nanoprobe, the fluorescent nanoprobe can be excited by low-energy near-infrared light, does not cause light damage, has higher biological tissue penetration depth, no background fluorescence interference and higher signal-to-noise ratio, and is a fluorescence detection method for veterinary drug residues with excellent reliability; therefore, the technical problem of low reliability of fluorescence detection of veterinary drug residues in the prior art is solved.

The application belongs to the field of microbial degradation of dyes, and relates to application of deinococcus in degradation of malachite green wastewater. The deinococcus is Deinococcus wulumuqiensis R12. When the initial concentration of malachite green MG is 200 mg / L, the cell concentration of R12 is 8.0 g / L, the reaction is carried out at 30 DEG C and pH 6.0 for 30 min, the degradation rate is 99.80%. When the initial concentration of MG is 1000 mg / L, the degradation rate can reach more than 95% after 1.5 h of reaction. Meanwhile, the R12 has wide adaptability to pH and temperature in degrading malachite green, and can efficiently degrade malachite green MG under the conditions of pH 5.0-10.0 and 10-45 DEG C.

The invention discloses a method for detecting malachite green, crystal violet and methylene blue, which is characterized in that two different haptens are respectively used as an immunogen and a coating antigen to obtain a high-sensitivity monoclonal antibody for resisting malachite green, crystal violet and methylene blue. The invention further establishes a chemiluminescence immune method for detecting malachite green, crystal violet and methylene blue based on biotin-streptavidin, and the method has the advantages of rapidness, sensitivity, low cost and high throughput, and is suitable for simultaneously detecting the residual quantity of malachite green, crystal violet or methylene blue in aquatic products.

The invention discloses a method for rapidly detecting malachite green in aquatic products, and belongs to the technical field of biological detection. According to the time-resolved fluorescent microsphere immunochromatography kit based on the specific nano-antibody, the nano-antibody is applied to immunochromatography, an optimized pretreatment method is combined, malachite green in aquatic products can be rapidly detected on site, sample pretreatment and detection can be completed within 15 min, the limit of detection (LOQ) is 0.03 mu g / kg, and the time-resolved fluorescent microsphere immunochromatography kit can be used for detecting malachite green in aquatic products. The naked eye line elimination value is 1.6 mu g / kg which is far higher than the detection level in the prior art, and the method has the advantages of high sensitivity, good specificity, simplicity in operation, short detection time and the like, and is suitable for on-site rapid screening of malachite green residues in aquatic products.

The invention discloses a triphenylmethane dye metabolite rapid detection method based on magnetic nano-enzyme selective oxidation and a preparation method of magnetic nano-enzyme, a novel nano-enzyme MnFe2O4 / PSS of sodium polystyrenesulfonate (PSS) modified magnetic ferrite (MnFe2O4) is prepared by adopting a coprecipitation method, the morphology and Zeta potential of the nano-enzyme are changed by doping PSS, and the magnetic nano-enzyme can be used for rapidly detecting the metabolite of the triphenylmethane dye. The nano-enzyme can react with leuco crystal violet and leuco malachite green more quickly, the leuco crystal violet and the leuco malachite green are oxidized into corresponding crystal violet and malachite green without further oxidation, after the reaction is completed, the error of a detection system can be reduced by utilizing magnetic separation, and by detecting the ultraviolet absorption peak intensity of oxidation products of the nano-enzyme, the detection accuracy is improved. A triphenylmethane dye metabolite oxidation-detection system is established, the magnetic nano-enzyme has good magnetism, the influence of the nano-enzyme on the absorbance of the system can be reduced through simple magnetic separation, and the purposes of reducing experimental errors and accurately detecting are achieved.

This invention provides a method for detecting malachite green veterinary drug residues using SERS based on silver-coated gold nanorods. The method includes: mixing a malachite green standard solution with silver-coated gold nanorods, adding a coagulant to form a mixed solution, and performing Raman spectroscopy to obtain the surface-enhanced Raman scattering (SERS) peaks of the malachite green standard solution; determining the characteristic peaks used to identify malachite green using SERS by assigning the malachite green molecular structure to the Raman peak positions; and determining the linear relationship between the concentration of malachite green and the intensity of the characteristic peaks. The method also involves mixing the sample solution to be tested with silver-coated gold nanorods, adding a coagulant to form a mixed solution, and performing Raman spectroscopy to obtain the peak intensity of the characteristic peaks of malachite green in the sample solution. The concentration of malachite green in the sample solution is calculated based on the linear relationship between the concentration of malachite green and the peak intensity of the characteristic peaks. This invention enables accurate and rapid detection of malachite green in aquatic products, ensuring the safe production of aquatic products.

This invention discloses a method for degrading dyes using dye decolorization peroxidase, belonging to the field of biotechnology. This invention expresses a DyP (RhDyPB) derived from the bacterium *Rhodococcus jostii* in *Escherichia coli*. By adjusting the pH, manganese ion, hydrogen peroxide, and Tween-80 content during the decolorization process, the decolorization efficiency of RhDyPB and *E. coli* expressing RhDyPB on the anthraquinone dye Reactive Blue 19, the oxanthracene dye Eosin Y, the indigo dye Indigo Carmine, and the triphenylmethane dye Malachite Green is effectively improved.

The invention relates to a fibrous protein ELISA (enzyme-linked immunosorbent assay) detection method based on homing peptide combined with malachite green, which comprises the following steps: S1, synthesizing a template chain and homing peptide to form a sensing probe; s2, adding a to-be-detected solution containing fibrin into an antibody solution to obtain a fibrin-antibody compound, and adding a sensing probe, a first primer and a second primer for incubation; s3, adding a first lock-type probe and a second lock-type probe for catalytic cyclization; s4, carrying out synchronous rolling circle transcription to obtain long-chain RNA respectively containing M1 and M2 sequence fragments; and S5, adding malachite green dye for incubation, and triggering a fluorescence signal. Compared with the prior art, by means of target fibrin mediation, dual recognition of the integrated antibody and the homing peptide on fibrin and signal generation induced by the split type MG-RNA aptamer, background noise is remarkably reduced, and specificity is remarkably improved.

The invention discloses an NH2-MIL-125 (Ti) nanocrystal adsorbent as well as a preparation method and application thereof, and belongs to the technical field of adsorption materials. The preparation method comprises the following steps: (1) mixing a hydrogen bond donor and a hydrogen bond acceptor to obtain a eutectic solvent; (2) adding the eutectic solvent into N, N-dimethylformamide to obtain a mixed solution; (3) adding an organic ligand into the mixed solution, stirring, and adding a metal titanium source to obtain a mixture; and (4) transferring the mixture into a reaction kettle, and carrying out solvothermal reaction to obtain the NH2-MIL-125 (Ti) nanocrystal adsorbent. The average particle size of the NH2-MIL-125 (Ti) nanocrystal adsorbent prepared by the invention is 50-175 nm, the shape of the NH2-MIL-125 (Ti) nanocrystal adsorbent is an irregular disc shape, and the specific surface area of the NH2-MIL-125 (Ti) nanocrystal The adsorbent can efficiently, rapidly and selectively adsorb cationic dyes in dye wastewater, the adsorption rates of rhodamine B and malachite green in an aqueous solution can reach 99.7% and 98.3% respectively, and the adsorbent has a wide pH application range and good cycle stability.

The present invention discloses a method for site-directed oxidative modification of RNA, which utilizes guide DNA to directionally guide the oxidation reaction to a preset guanine site in the post-transcriptional stage. LOCAL technology achieves site-selective oxidation with near-stoichiometric efficiency and achieves accuracy close to that of a single nucleotide by enhancing the reactivity of unpaired nucleotides in DNA-induced ring structures. The present invention systematically optimizes the guide DNA and reaction conditions, and preliminarily reveals the reaction rules that depend on sequence and structure in the oxidation reaction. The present invention uses the malachite green aptamer as a model, implements site-selective oxidation near its ligand binding site, and finds that its fluorescence generation function is completely lost, which strongly proves that specific oxidative damage can lead to impaired RNA function. This modular approach can also be adapted to biocoupling strategies through click chemistry for intracellular RNA visualization and affinity enrichment studies.

The present invention relates to the technical field of fluorescent probes, and in particular to a fluorescent probe, test paper, and preparation method and application thereof for detecting malachite green, a triphenylmethane compound. The fluorescent probe comprises a fluorescent ion pair formed by rhodamine B (RB) and perfluorinated tetraphenylborate ion (F5TPB); the triphenylmethane compounds are malachite green (MG), crystal violet (CV), and methyl violet (MV); the detection limit for malachite green is 0.1776 μmol / L (3σ / k, n=11); the present invention also provides a test paper for detecting triphenylmethane compounds, which can simply and conveniently detect malachite green and its residual amount.

The invention belongs to the technical field of chemistry and new materials, and particularly relates to preparation of a carboxylic acid functionalized porous organic polymer for adsorbing cationic dye and application of the carboxylic acid functionalized porous organic polymer in dye adsorption. The carboxylic acid functionalized porous organic polymer LNU-81 is prepared by taking octaphenyl-POSS as a raw material and taking pyromellitic dianhydride as a reaction external cross-linking agent. The porous organic polymer has the characteristics of a pi-conjugated aromatic system, a hierarchical pore structure, an electronegative skeleton and the like, so that the LNU-81 shows excellent adsorption capacity on malachite green under the synergistic effect of pi-pi accumulation action and hydrogen bond and electrostatic interaction, and the highest adsorption capacity is 1945.36 mg g <-1 >. The carboxyl skeleton shows strong affinity to malachite green, ensures rapid adsorption kinetics, and is beneficial for obtaining higher adsorption efficiency of the material. The invention provides a novel method for directionally designing and preparing the porous organic polymer with high adsorption performance.

This invention belongs to the field of environmental pollution detection and treatment technology, specifically relating to a SiO2@Ag / ZnO flexible substrate and its application in the detection and degradation of malachite green. A SiO2@Ag nanocomposite material is prepared by modifying Ag nanoparticles onto the surface of SiO2 spheres. Further, ZnO is attached to the surface of SiO2@Ag to prepare the composite material SiO2@Ag / ZnO. A SiO2@Ag / ZnO flexible substrate was successfully prepared by attaching SiO2@Ag / ZnO to fabric. This material exhibits detection capability at lower concentrations and demonstrates stable response to concentration changes in SERS signal detection of malachite green, exhibiting high quantitative accuracy and suitability for SERS quantitative analysis. Simultaneously, this material achieves stable degradation of malachite green under light irradiation conditions; the degradation rate gradually increases with prolonged ultraviolet light irradiation time, reaching a maximum degradation rate of 91.1%.

The invention discloses a preparation method of a carboxyl-functionalized porous organic polymer adsorbent and application of the carboxyl-functionalized porous organic polymer adsorbent in the field of dye wastewater. According to the method, dimethyl formal is used as an external cross-linking agent, LNU-80 is synthesized through a Friedel-Crafts coupling reaction, and a hydrophilic group is introduced into an LNU-80 framework, so that the carboxyl functionalized porous organic polymer LNU-80-COOH is obtained. Experimental results show that LNU-80-COOH shows excellent adsorption performance on malachite green, and the adsorption capacity of the LNU-80-COOH under the room temperature condition reaches up to 4375.9 mg g <-1 >. After five times of circulation, the adsorption efficiency of the LNU-80-COOH on the malachite green is still kept at 90% or above. The invention provides a novel method for directionally designing and preparing the porous organic polymer with high adsorption selectivity. The adsorbent has excellent adsorption selectivity and cycling stability, and has a wide development prospect in the field of dye wastewater treatment.

The present application relates to the technical field of immunochromatographic analysis, and discloses a signal enhancer for a colloidal gold immunochromatographic rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, signal amplification is realized by catalytic deposition of silver nanoparticles on the surface of colloidal gold, the present application is simple to operate, is added at one time, and reaction is completed in 5-10 minutes; sensitivity is improved by 5-10 times, specificity is higher than 98%; stability is excellent, 12 months of storage at 4 DEG C; it is compatible with various colloidal gold rapid detection cards; cost is controllable, single detection is increased by 0.3-0.5 yuan, and the present application is especially suitable for super-sensitive detection of small molecule substances in the field of food safety, such as detection limit of malachite green of 0.1 microgram per liter, detection limit of fipronil of 0.5 microgram per liter, and the application range of the colloidal gold immunochromatographic rapid detection card is significantly expanded.