6 results about "Thioflavin" patented technology

The invention belongs to the field of fluorescence sensors, relates to detection of tobramycin, and discloses a double-state probe switch type fluorescence sensor as well as a detection method and application thereof. According to the technology, quantitative analysis can be realized without enzyme catalysis or nucleic acid labeling through dynamic balance regulation and control of a G-quadruplex and thioflavin fluorescent system and CHA. The core mechanism is based on base pairing of Apt and cDNA: in the absence of TOB, Apt-cDNA forms a stable double-helix structure, so that HP2-5 rich in G bases spontaneously forms a G-quadruplex, and strong fluorescence is generated after ThT is embedded; according to the sensor, the CHA effect and G-quadruplex dynamic coordinated regulation and control are realized for the first time, complex steps such as enzyme catalysis and fluorescence labeling are not needed in an experiment, the sensor has low detection limit and high accuracy, can be accurately applied to actual sample detection, and a practical solution is provided for detection of TOB residues in animal-derived food.

The application belongs to the technical field of fluorescent probe detection, and particularly relates to a kind of blue fluorescent carbon quantum dots and its application. The application takes thioflavin T as raw material, ethanol as solvent, and synthesizes blue fluorescent carbon quantum dots with large conjugated structure through simple one-step microwave method. The carbon quantum dots are difficult to dissolve in water and easy to dissolve in organic solvents, and have sensitive fluorescence response to ethanol in the mixed solution of ethanol and water. Using the carbon quantum dots as fluorescent probe, the rapid and efficient detection of ethanol content in liquor can be realized by using ordinary fluorescence spectrometer, and the application has the advantages of convenient operation, high sensitivity, short response time and the like, and has broad application potential to develop portable alcohol detection scheme.

The invention belongs to the field of biosensors, and particularly relates to a label-free fluorescence resonance energy transfer (FRET) enhanced fluorescent histamine RNA biosensor. A histamine response type fluorescent RNA switch sequence is constructed, a histamine RNA aptamer and a Pepper fluorescent RNA aptamer are integrated into a whole through a connecting sequence, thioflavin T is introduced to serve as an energy donor, HBC530 serves as a receptor ligand, a label-free FRET pair is formed, the background is reduced, response is amplified, the signal-to-noise ratio is remarkably increased, and rapid, low-cost and fluorescent quantitative detection of histamine is achieved.

The application belongs to a kind of difluoroborane hydrazine pyrrole fluorescent probe and its preparation method and beta amyloid detection application, belongs to the field of fluorescence detection technology. The probe is composed of BOPHY fluorescent mother body and recognition group, and its preparation method includes three steps of BOPHY precursor preparation, fluorescent mother body synthesis and target probe molecule synthesis. The prepared series of probes have large Stokes shift, can effectively reduce imaging interference, and show high sensitivity and high selectivity of fluorescence response to A beta aggregate, wherein the performance of N-BOPHY is optimal. Experiments show that the binding affinity of the probe to A beta aggregate is high, the staining imaging result of A beta plaque in brain slice has high colocalization with commercial probe thioflavin-T, and the cell toxicity is low and the biocompatibility is good.

Provided herein are methods of determining a cognitive status of a test subject. In some embodiments, the methods include passing a test subject data set through a model that relates test subject data sets to cognitive status of the test subjects in which the model was created using reference subject data sets produced by determining three or more features from samples obtained from the reference subjects using at least a thioflavin T (ThT) assay, a dynamic light scattering (DLS) assay, and a neurotoxicity assay. In some embodiments, the methods also include outputting from the model a classification of the test subject as having a Parkinson's disease (PD) status of PD-NC (normal cognition) or PD-CI (cognitive impairment). Related systems, computer readable media, and additional methods are also provided.

The application discloses a hydrogel for improving the precision of photocuring 3D printing and application thereof. The application finds that the precision of photocuring 3D printing can be improved by adding a pigment such as thioflavin T, alizarin yellow R, methylene blue and / or purple cabbage into an ink containing a photo-crosslinking hydrogel such as methacrylated gelatin (GelMA) as a photocuring stabilizer. Based on this, the application provides a hydrogel ink capable of improving the precision of photocuring 3D printing, and by mixing the photo-crosslinking hydrogel with a photo initiator and a photocuring stabilizer in proportion, the entity obtained by printing using the hydrogel ink has higher precision. In addition, the operation of 3D printing using the hydrogel ink is simple, has strong practicability, and is beneficial to the construction of repair materials required in the biomedical field such as tissue engineering.