95 results about "Methyl blue" patented technology

The invention relates to a method for preparing a magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material by a one-step method and an application of the magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material, and aims to solve the problems of poor catalytic activity, complex separation and recovery and complex synthesis method of the existing PMS activated catalytic material. According to the method, melamine is selected as a carbon source and a nitrogen source, ferric nitrate and copper sulfate are selected as an iron source and a copper source respectively, and the magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material is synthesized in one step by adopting a high-temperature pyrolysis method. The adsorbent not only has excellent adsorption performance on dye-methylene blue, but also can realize high-efficiency oxidative degradation. The magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material prepared by the invention not only has excellent catalytic performance, but also is easy to magnetically separate and recycle, and is suitable for efficient treatment of dye wastewater. The method is applied to the field of catalytic degradation of wastewater.

The application discloses a high-efficiency degradation of dyes and antibiotics high-entropy spinel ferrite catalyst and a preparation method and application thereof. 1 / 2 Zn 1 / 2 Fe 2 / 3Co 2 / 3 Ni 2 / 3 O4(HEO-FNCCZ) high-entropy spinel ferrite catalyst, the photocatalytic degradation performance of common dyes and antibiotics is evaluated by using simulated visible light photocatalysis, and the reaction mechanism of the photocatalytic degradation of methylene blue with the best effect is studied. The catalytic effect of the spinel ferrite is utilized to effectively promote the photocatalytic reaction, so that the environment-friendly pollutant degradation is realized. The method has the characteristics of simple production process, no introduction of various organic toxic and harmful substances, non-toxic and harmless in use, catalyst recovery efficiency more than 95%, and no secondary pollution. The preparation method only involves processes such as dissolving, calcining and crushing, and is extremely suitable for industrialized batch production.

The invention discloses a black phosphorus-methylene blue nano composition for improving blood brain permeability and a synthesis method thereof. The synthesis method comprises the following steps: step 1, mixing black phosphorus nanosheets with polyethylene glycol for reaction; step 2, carrying out centrifugal cleaning on the reaction liquid in the step 1, and collecting a lower-layer reactant; step 3, adding aminated superparamagnetic nanoparticles and methylene blue into the lower reactant obtained in the step 2 for reaction; and 4, carrying out centrifugal cleaning on the reaction liquid obtained in the step 3, and collecting a lower-layer reactant. The invention also discloses the black phosphorus-methylene blue nano composition synthesized by the synthesis method, the drug loading rate of methylene blue in the composition exceeds 60%, and the composition has good biological safety, is non-toxic to cells and mice, is very simple and convenient in preparation process, and has a wide application prospect in the aspect of improving the blood-brain barrier permeability.

This invention discloses a molecularly switched adsorbent for adsorbing triphenylmethane dyes and its application, belonging to the field of wastewater treatment technology. The molecularly switched adsorbent proposed in this invention can rapidly remove triphenylmethane dyes such as crystal violet, malachite green, and methylene blue dyes from water; it can remove tens of ppm concentrations of crystal violet, malachite green, and methylene blue dyes from water in a very short time (90 min), with a removal rate of ≥92%. Moreover, this adsorbent can maintain a removal rate of ≥90% under a wide pH range (6-11) and high ion concentration conditions (1M). The activation and regeneration conditions of the adsorbent of this invention are mild; dye molecules can be removed by elution in a low-concentration alkaline solution only a few times. The adsorbent can be continuously recycled at least 10 times without significant attenuation of adsorption capacity.

The present invention belongs to the field of biomedicine technology and discloses a nanomotor drug-loaded particle and its preparation method and application. The nanomotor drug-loaded particle is formed by loading platinum nanoparticles and methylene blue drugs on a DNA tetrahedron structure. The nanomotor drug-loaded particle can enhance the uptake of the photosensitizer methylene blue (MB) by tumor cells while achieving deep penetration into tumors, thereby reducing the amount of drugs required for conventional systemic cancer treatment. The nanomotor drug-loaded particles are safer and have fewer adverse reactions. The nanomotor drug-loaded particles provided by the present invention have a reliable source, are simple to prepare, have high biocompatibility, specifically respond to photodynamic therapy, and effectively inhibit tumor cell growth.

This invention belongs to the field of SERS detection technology, specifically relating to an Ag-Cu 0.5 Zn 0.5 Application of Fe2O4 composite SERS substrate in the detection of methylene blue in wastewater. First, Cu was prepared using a calcination method. 0.5 Zn 0.5 Fe2O4 nanomaterials, and then Ag NPs are grown on Cu 0.5 Zn 0.5 Ag-Cu was prepared on Fe2O4 support surface. 0.5 Zn 0.5 Fe2O4 composite SERS substrate was loaded onto a glass slide. This substrate integrates the LSPR effect of Ag NPs and Cu. 0.5 Zn 0.5 The cation rearrangement and lattice distortion effects introduced by Fe2O4 optimize the separation and migration behavior of photogenerated charges. The abundant defect states and tunable electronic structure brought about by Cu / Zn synergistic regulation are conducive to constructing efficient interfacial charge transfer channels, thereby enhancing the Raman signal and enabling the detection of trace amounts of methylene blue in wastewater.

The invention discloses a quercetin composite methyl blue light catalyst and a preparation method and application thereof, and relates to the technical field of photocatalyst preparation, and the preparation method comprises the following steps: S1, placing quercetin and methyl blue in a beaker, adding deionized water, then adding H2SO4 and absolute ethyl alcohol, and stirring to obtain a mixed solution; s2, transferring the mixed solution into a hydrothermal reaction kettle with a polytetrafluoroethylene lining for constant-temperature reaction; and S3, after the constant-temperature reaction is finished, cooling to room temperature, washing a reaction product with deionized water, and then drying and grinding the reaction product into powder. According to the photocatalyst disclosed by the invention, a stable compound structure is constructed by utilizing the dihydrogen-bond interaction between hydroxyl of quercetin and sulfo and amino of methyl blue, and the photocatalytic activity is remarkably enhanced. Under the irradiation of visible light, the prepared photocatalyst shows excellent lignite depolymerization performance, and humic acid and fulvic acid rich in hydroxyl and amino can be efficiently generated.

The present invention relates to a method for improving the detection performance of an electrochemical biosensor and its application in a ratiometric electrochemical biosensor. Methylene blue and cholesterol-modified double-stranded DNA probes are immobilized on a gold electrode modified with a 1-hexanethiol assembly layer via hydrophobic interaction, thereby improving the stability of the methylene blue at the gold electrode interface and the immobilization efficiency of the double-stranded DNA probe at the gold electrode interface. In a potassium ferricyanide-mediated electrocatalytic system, the methylene blue on the gold electrode serves as a signal unit, and ferrocene is introduced as another signal unit. The 1-hexanethiol assembly layer on the gold electrode can shield [Fe(CN)6] 3‒ Electron transfer between the electrode interface and methylene blue enables simultaneous signal amplification of both methylene blue and ferrocene. Methylene blue, acting as an electron mediator, enhances the signal response of ferrocene, achieving secondary signal amplification of ferrocene, thereby improving sensor detection sensitivity. The ratiometric electrochemical biosensor constructed using this method exhibits excellent sensitivity, accuracy, and selectivity.

The invention provides a living cell surface double-tumor marker in-situ detection method based on a closed bipolar electrode (cBPE) array and an electrochemiluminescence (ECL) technology. The living cell surface double-tumor marker in-situ detection method is used for synchronously quantifying carcino-embryonic antigen (CEA) and alpha-fetoprotein (AFP). According to the system, the high specific surface area, the ordered porous structure and the rich surface amino active sites of a covalent organic framework (COF) material are utilized, and the excellent conductivity and biocompatibility of gold nanoparticles (Au NPs) are combined, so that the bifunctional signal probe COF (at) Au (at) MBApt is constructed. The probe can simultaneously load a large number of signal molecule methylene blue (MB) and is coupled with a targeting aptamer (Apt), so that high-capacity labeling and specific recognition of a target marker are realized.

The invention discloses a preparation method of a CdIn2S4 and InVO4 composite material for promoting photocatalytic degradation of an organic dye methylene blue, InVO4 is synthesized through a hydrothermal reaction, then spherical InVO4 is modified on the surface of spherical CdIn2S4, and a unique peanut-shaped CIS-2IVO composite material is successfully constructed, so that the light absorption capacity of CIS-2IVO is enhanced, the conversion from light energy to chemical energy is promoted, and the photocatalytic degradation of the organic dye methylene blue is promoted. The separation and migration efficiency of photon-generated carriers is remarkably improved, the compound loss of electron-hole pairs is reduced, the chemical stability of the composite material is enhanced, and the loss of active components in the reaction process is effectively inhibited, so that the service life of the catalyst is prolonged while the degradation efficiency is greatly improved, and the cost is reduced. And a practical and innovative solution is provided for efficient photocatalytic degradation of organic pollutants. The method is easy and convenient to operate and high in controllability, and the light response range is effectively widened.

This invention discloses a method for measuring methylene blue based on the inner filter effect of chlorophyll carbon quantum dots (CQDs). This method uses CQDs as fluorescent probes and exploits the inner filter effect of CQD fluorescence quenching to detect the CQD content. The method is simple and convenient to operate, highly sensitive, and can rapidly complete sample determination. The CQDs are produced from biomass, resulting in low cost and broad application prospects.

The application relates to the technical field of full-color photopolymer, and specifically discloses a full-color photopolymer film, a preparation method and application thereof. The full-color photopolymer film provided by the application specifically comprises 40-50 parts of writing monomers, 0.8-1.2 parts of photosensitizers, 4-7.2 parts of photoinitiators, 14-16 parts of polycaprolactone glycol, 21-25 parts of aliphatic polyether isocyanate, 4-6 parts of crosslinking agents, 0.3-0.6 parts of defoaming agents, 0.8-1.2 parts of anti-oxidation inhibitors, 0.8-1.2 parts of catalysts and 5-8 parts of solvents; the photosensitizer is composed of 2,5-bis((2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)methylene)cyclopentan-1-one and methylene blue. By using the technical scheme, the full-color photopolymer with high red, blue and green light diffraction efficiency and high refractive index modulation degree can be obtained.

The invention provides a molecular classifier for detecting gastric cancer miRNA. A preparation method of the molecular classifier comprises the following steps: depositing a PABA modification layer on the surface of a screen-printed electrode; activating carboxyl on the surface of the para aminobenzoic acid modification layer by adopting an EDC / NHS composite system; carrying out amination modification on an MXene material, and coating the surface of the activated para aminobenzoic acid modification layer with the modified MXene material to form an MXene functional layer; depositing gold nanoparticles on the surface of the MXene functional layer to obtain an electrochemical activity sensing interface; a signal reporter molecule is combined with an aptamer probe, the signal reporter molecule is a DNA tetrahedron-methylene blue complex, and the combined complex is dropwise added on the surface of an electrochemical activity sensing interface, so that the aptamer probe is combined on the surface of the electrochemical activity sensing interface. The molecular classifier can be used for preparing gastric cancer miRNA detection products, and the problems that in the prior art, a classifier is high in complexity and high in signal leakage risk are solved.

The present application relates to a kind of functional materials for organic dye adsorption and degradation and its preparation method and application, belong to pollutant degradation field.The present application uses one-step hydrothermal synthesis method, with anhydrous ferric chloride, tin chloride tetrahydrate and sodium hydroxide as raw material, successfully constructs the Fe (Sn (OH) 6) / FeSnO (OH) 5 heterojunction composite material with close interface combination.The material can realize the efficient removal of typical organic pollutants methylene blue at normal temperature and pressure, and the degradation rate can reach more than 90% in a short time, showing excellent adsorption and degradation performance.The present application has simple process, mild reaction condition, low raw material cost, avoids complex post-processing process, has good scalability and application potential.The material can provide an efficient, economical technical solution for the treatment of refractory organic pollutants in water, has significant environmental benefits and practical application value.

This invention discloses a quercetin-methylene blue composite photocatalyst, its preparation method, and its application, relating to the field of photocatalyst preparation technology. The method includes the following steps: S1: Quercetin and methylene blue are placed in a beaker, deionized water is added, followed by H2SO4 and anhydrous ethanol, and the mixture is stirred to obtain a mixed solution; S2: The mixed solution is transferred to a hydrothermal reactor with a polytetrafluoroethylene liner for a isothermal reaction; S3: After the isothermal reaction, the mixture is cooled to room temperature, the reaction product is washed with deionized water, and then dried and ground into powder to obtain the final product. The photocatalyst of this invention utilizes the double hydrogen bond between the hydroxyl groups of quercetin and the sulfonic acid and amino groups of methylene blue to construct a stable composite structure, significantly enhancing its photocatalytic activity. Under visible light irradiation, the prepared photocatalyst exhibits excellent depolymerization performance in lignite, efficiently generating humic acid and fulvic acid rich in hydroxyl and amino groups.

The invention relates to the field of biotechnology and dead / living cell detection, in particular to a method for rapidly determining the activity of biological membrane cells in immobilized fermentation. According to the method, a methylene blue and trisodium citrate synergistic chromosome system is optimized, a biofilm EPS matrix is synergistically penetrated by virtue of the dispersion effect of trisodium citrate and the reduction characteristic of methylene blue, and a spectrophotometric method is combined to detect the OD value of a staining solution instead of stained cells, so that the efficient quantitative analysis of the ratio of dead cells to living cells of the immobilized biofilm is realized. The method can complete detection within 200 s, is simple and convenient to operate and low in cost, does not need complex equipment, and is remarkably superior to a traditional fluorescent staining method.

The application belongs to the technical field of biosensing detection, and particularly relates to a preparation method of a photoelectrochemical sensor based on a methylene blue composite material loaded by Ni-MOF and application thereof. The application introduces a green Ni-MOF to fix a photosensitive molecule methylene blue, and SA-ALP catalyzes an electron donor ascorbic acid (AA) generated by ascorbic acid phosphate, thereby constructing a spatially confined aptamer sensor of the methylene blue composite material loaded by Ni-MOF, realizing the improvement of the sensitivity of the detection of MC-LR, and the sensor has good selectivity and stability. The spatially confined photoelectrochemical aptamer sensor developed by the application can realize the rapid, high-selectivity and sensitive analysis of the detection of MC-LR by adjusting the content of AA, and a good recovery rate is obtained.

The invention provides clostridium pasteurianum loaded with nano-copper and application of the clostridium pasteurianum in catalytic degradation of methylene blue. The clostridium pasteurianum loaded with nano-copper is obtained by adsorbing a clostridium pasteurianum solution with a copper solution under an anaerobic condition and then introducing hydrogen as a reducing agent for reaction; according to the clostridium pasteurianum loaded with the nano-copper, the loading capacity of the nano-copper is 25.33 mmol / g to 27.33 mmol / g, and the average particle size of the nano-copper is 42.28 + / -12.14 nm. The functional clostridium pasteurianum with nano-copper loaded in cells is constructed, methylene blue in an aqueous solution is degraded to serve as a template for reaction to verify the catalytic capacity of the clostridium pasteurianum, and a certain supporting effect is expected to be provided for biological preparation of a nano-metal catalyst.

The invention discloses a preparation method of Bi2WO6 / Bi4Ti3O12 for synergistically promoting photocatalytic degradation of an organic dye methylene blue, irregular Bi4Ti3O12 nanosheets are loaded on Bi2WO6 nanospheres by using a hydrothermal method, the Bi2WO6 nanospheres are used for degrading methylene blue, nanocrystallized Bi2WO6 and Bi4Ti3O12 of a nanosheet structure have larger specific surface areas, more surface active sites are exposed, and the photocatalytic degradation of the organic dye methylene blue is promoted. The adsorption and catalytic degradation of methylene blue molecules are facilitated. The preparation process is simple, the degradation effect is excellent, and 99.9% of 10 mg L <-1 > methylene blue solution is finally degraded within 180 min through optimization of the initial concentration and pH.

This invention relates to the field of biomedical technology, specifically to an antifungal nanoparticle, a combination drug, and its application. The antifungal nanoparticle is prepared by covalently linking the aptamer AU1 to drug-loaded silk fibroin nanoparticles via amide bonds. The mass ratio of the drug-loaded silk fibroin nanoparticles to the aptamer AU1 is 20:0.5~2.0. The antifungal nanoparticles of this invention are prepared by a desolvation method using voriconazole-loaded silk fibroin nanoparticles, and the targeting aptamer AU1 is attached to their surface using a carbodiimide chemical cross-linking method. This allows for specific recognition of Candida albicans, increasing drug accumulation at the infection site. Further combination with methylene blue-mediated photodynamic therapy can disrupt biofilm structures, enhance nanoparticle penetration, achieve synergistic bactericidal activity, and reduce systemic toxicity, providing a novel, highly effective, and low-toxicity treatment strategy for fungal biofilm infections.

The preparation method comprises the following steps: adding ammonium citrate as a reducing agent, polyvinylpyrrolidone as a dispersing agent and carboxymethyl cellulose as an adhesive, carrying out hydrothermal reduction on graphene to self-assemble gel, adding ultrasonic-treated multi-walled carbon nanotubes to prepare a multi-walled carbon nanotube / graphene suspension, and finally, carrying out freeze drying to prepare the aerogel. The multi-walled carbon nanotube / graphene composite aerogel prepared through freeze drying in short hydrothermal time and large-scale production has the advantages of ultralight weight, low density and high methylene blue dye pollutant adsorption performance.

The application relates to the technical field of detection, in particular to a general foodborne pathogenic bacteria detection method suitable for a long-distance sailing environment. UiO-66 is used to enrich methylene blue, and is combined with target bacteria aptamer to obtain UiO-66 / MB / Aptamer which simultaneously serves as a signal amplification and recognition probe. A membrane separation method is used to filter out the probes not combined with the bacteria. The electrochemical signal of methylene blue is detected by using a differential pulse voltammetry method, a corresponding linear relationship is constructed, and the concentration of the bacteria is quantitatively detected. The application not only guarantees the sensitivity, detection time, portability and other characteristics of the detection of foodborne pathogenic bacteria, but also provides a general detection method for detecting various target bacteria. Through the reproducibility and stability test under the high-temperature, high-humidity and high-salt environment, it is proved that the application can realize the rapid, sensitive and stable detection of foodborne pathogenic bacteria in a complex long-distance sailing environment.

The invention discloses a calibration method of a nucleic acid quantitative fluorometer, which adopts a methylene blue aqueous solution with stable chemical property as a standard substance to replace a biological nucleic acid reagent, and verifies the optical performance of the nucleic acid quantitative fluorometer by constructing a linear response relationship of a chemical standard substance. The method comprises the following steps: preparing a methylene blue mother solution and a gradient calibration solution, and carrying out light-shielding standing and thermodynamic stabilization treatment; collecting a fluorescence signal by using a nucleic acid quantitative fluorometer to construct a standard curve; selecting a verification sample for reverse measurement, and calculating an indication error, a channel range and measurement repeatability; and finally, judging whether the calibration result is qualified or not through hierarchical logic including linear validity verification and metrological characteristic subentry verification. The problems that an existing RNA kit is high in calibration cost, poor in linearity and unstable in result are solved, and the method has the advantages of being low in cost, easy and convenient to operate, high in result confidence coefficient and the like.

Disclosed in the present invention are the preparation and use of a supported photo-Fenton catalyst. The preparation method comprises: I, formulating a sodium tungstate solution and a potassium oxalate solution, adjusting the pH until acidic, and performing a hydrothermal reaction and calcination to obtain a tungsten trioxide composite material; II, formulating a sodium tungstate solution and an ammonium ferrous sulfate solution; III, ultrasonically dispersing the tungsten trioxide composite material in the ammonium ferrous sulfate solution to obtain a suspension; and IV, under continuous stirring, adding the sodium tungstate solution into the suspension in a dropwise manner, stirring same, performing a hydrothermal reaction, and washing and drying same to obtain a tungsten trioxide / iron tungstate nano material. In the present invention, an FeWO4 / WO3 heterojunction is formed by using a hydrothermal synthesis method, which improves the separation efficiency of carriers and the photo-Fenton catalyst activity of the tungsten trioxide / iron tungstate nano material, thereby increasing the catalytic efficiency thereof. The catalyst is suitable for the degradation treatment of photocatalyst wastewater or dye methylene blue and methyl violet, and the preparation process is simple and easy to implement, and has low pollution.

The invention discloses a preparation method of an Er-doped CdIn2S4 photocatalyst for photocatalytic degradation of methylene blue, which comprises the following steps: adding cadmium chloride, indium chloride tetrahydrate and thioacetamide into deionized water, and carrying out hydrothermal reaction to prepare the CdIn2S4 photocatalyst; in the same reaction system, Er (NO3) 3.5 H2O is added according to different proportions of the molar weight of indium trichloride tetrahydrate, and three samples with different Er doping concentrations are obtained. The photocatalytic performance of CdIn2S4 is effectively regulated and controlled through Er doping, the separation efficiency of photon-generated carriers is remarkably improved, and meanwhile the light absorption capacity of the material is enhanced. More adsorption sites and active sites are exposed on the surface of the modified catalyst, so that the degradation efficiency of methylene blue is greatly improved. The method has the remarkable advantages of being simple in preparation process, low in cost, easy to recycle and the like, and shows a good application prospect in the field of dye wastewater treatment.

The application discloses a kind of long afterglow base ratio type aptamer sensor construction and carbendazim detection application, belong to spectral analysis technical field.The application is based on zinc germanate manganese-doped long afterglow material (ZGM) and the methylene blue with fluorescent characteristic constructs the ratio type aptamer sensor without complementary chain, using the overlapping characteristics of ultraviolet absorption spectrum and ZGM phosphorescence emission spectrum of methylene blue, through resonance energy transfer mechanism, realize the ZGM phosphorescence quenching and methylene blue fluorescence enhancement of probe, and then construct the long afterglow ratio type aptamer sensor without complementary chain.The characteristic emission peak of the long afterglow ratio type aptamer sensor is at the green phosphorescence emission of 542nm and the fluorescence emission of 700nm, the probe has the advantages of without in-situ excitation, without background interference and exogenous interference, high specificity, can sensitively, accurately and specifically detect CBZ residue in food sample.

This utility model relates to the field of anti-counterfeiting label technology and discloses a double-sided printed anti-counterfeiting label, including a substrate isolation layer and a protective film installed on the substrate isolation layer, a second label layer, a first label layer, and a main anti-counterfeiting layer. Through the interaction of the brittle film layer, the cavity, and the microcapsule dye inside the cavity, when the customer completes the anti-counterfeiting code verification, they can directly press and break the brittle film layer, and then directly apply force to the microcapsule dye to break it, allowing the methylene blue dye inside to cover the anti-counterfeiting layer, destroying its function and making it unusable. The structure is simple, easy to operate, and highly practical.

The invention discloses a simple preparation and high-efficiency recovery method of polymethylene blue based on composite printing and dyeing wastewater, which realizes high-efficiency recovery of polymethylene blue in composite printing and dyeing wastewater through a multi-stage grid, an adjusting tank, a reaction tank, a sedimentation tank and a dehydration room. Adjusting the composite printing and dyeing wastewater in the adjusting tank to be acidic, reacting the composite printing and dyeing wastewater with peroxydisulfate in a reaction tank, performing high-speed stirring, low-speed stirring and standing separation, decolorizing liquid-phase methylene blue, and depositing solid-phase polymethylene blue; under the action of the sedimentation tank, solid-phase polymethylene blue forms a filter cake, and the filter cake can be stored in the shade for later use after being subjected to vacuum drying. Compared with other dye recovery methods, the method has the advantages of simplicity in operation, low cost, safety, high efficiency, no secondary pollution, wide applicable concentration range and the like, realizes specific recovery of polymethylene blue in the composite printing and dyeing wastewater, and has wide engineering application prospects.

The invention discloses a leucine aminopeptidase fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of fine chemical engineering, the probe takes a fluorescent dye methylene blue MB as a structural basis, and the final fluorescent probe for detecting leucine aminopeptidase LAP is constructed by introducing an LAP recognition group L-leucine to destroy a conjugated structure of the fluorescent dye methylene blue MB. After the fluorescent probe with the structural formula of # imgabs0 # responds to LAP, the recognition group leaves, and the probe recovers the conjugated structure of MB, so that strong near-infrared fluorescence is shown at 690nm, and the detection limit of 1.65 mU / mL is realized; the LAP can specifically recognize N-terminal residues of L-leucine in a probe structure, so that the probe shows high selectivity on LAP detection; meanwhile, MB is used as a dye with photodynamic performance, and the photodynamic performance of MB is activated after the probe and LAP act; finally, the probe is successfully used for fluorescence imaging and photodynamic therapy of cancer cells.