69 results about "Methyl blue" patented technology

The application discloses a high-efficiency degradation of dyes and antibiotics high-entropy spinel ferrite catalyst and a preparation method and application thereof. 1 / 2 Zn 1 / 2 Fe 2 / 3Co 2 / 3 Ni 2 / 3 O4(HEO-FNCCZ) high-entropy spinel ferrite catalyst, the photocatalytic degradation performance of common dyes and antibiotics is evaluated by using simulated visible light photocatalysis, and the reaction mechanism of the photocatalytic degradation of methylene blue with the best effect is studied. The catalytic effect of the spinel ferrite is utilized to effectively promote the photocatalytic reaction, so that the environment-friendly pollutant degradation is realized. The method has the characteristics of simple production process, no introduction of various organic toxic and harmful substances, non-toxic and harmless in use, catalyst recovery efficiency more than 95%, and no secondary pollution. The preparation method only involves processes such as dissolving, calcining and crushing, and is extremely suitable for industrialized batch production.

The invention discloses a black phosphorus-methylene blue nano composition for improving blood brain permeability and a synthesis method thereof. The synthesis method comprises the following steps: step 1, mixing black phosphorus nanosheets with polyethylene glycol for reaction; step 2, carrying out centrifugal cleaning on the reaction liquid in the step 1, and collecting a lower-layer reactant; step 3, adding aminated superparamagnetic nanoparticles and methylene blue into the lower reactant obtained in the step 2 for reaction; and 4, carrying out centrifugal cleaning on the reaction liquid obtained in the step 3, and collecting a lower-layer reactant. The invention also discloses the black phosphorus-methylene blue nano composition synthesized by the synthesis method, the drug loading rate of methylene blue in the composition exceeds 60%, and the composition has good biological safety, is non-toxic to cells and mice, is very simple and convenient in preparation process, and has a wide application prospect in the aspect of improving the blood-brain barrier permeability.

This invention belongs to the field of SERS detection technology, specifically relating to an Ag-Cu 0.5 Zn 0.5 Application of Fe2O4 composite SERS substrate in the detection of methylene blue in wastewater. First, Cu was prepared using a calcination method. 0.5 Zn 0.5 Fe2O4 nanomaterials, and then Ag NPs are grown on Cu 0.5 Zn 0.5 Ag-Cu was prepared on Fe2O4 support surface. 0.5 Zn 0.5 Fe2O4 composite SERS substrate was loaded onto a glass slide. This substrate integrates the LSPR effect of Ag NPs and Cu. 0.5 Zn 0.5 The cation rearrangement and lattice distortion effects introduced by Fe2O4 optimize the separation and migration behavior of photogenerated charges. The abundant defect states and tunable electronic structure brought about by Cu / Zn synergistic regulation are conducive to constructing efficient interfacial charge transfer channels, thereby enhancing the Raman signal and enabling the detection of trace amounts of methylene blue in wastewater.

The invention discloses a quercetin composite methyl blue light catalyst and a preparation method and application thereof, and relates to the technical field of photocatalyst preparation, and the preparation method comprises the following steps: S1, placing quercetin and methyl blue in a beaker, adding deionized water, then adding H2SO4 and absolute ethyl alcohol, and stirring to obtain a mixed solution; s2, transferring the mixed solution into a hydrothermal reaction kettle with a polytetrafluoroethylene lining for constant-temperature reaction; and S3, after the constant-temperature reaction is finished, cooling to room temperature, washing a reaction product with deionized water, and then drying and grinding the reaction product into powder. According to the photocatalyst disclosed by the invention, a stable compound structure is constructed by utilizing the dihydrogen-bond interaction between hydroxyl of quercetin and sulfo and amino of methyl blue, and the photocatalytic activity is remarkably enhanced. Under the irradiation of visible light, the prepared photocatalyst shows excellent lignite depolymerization performance, and humic acid and fulvic acid rich in hydroxyl and amino can be efficiently generated.

The invention provides a living cell surface double-tumor marker in-situ detection method based on a closed bipolar electrode (cBPE) array and an electrochemiluminescence (ECL) technology. The living cell surface double-tumor marker in-situ detection method is used for synchronously quantifying carcino-embryonic antigen (CEA) and alpha-fetoprotein (AFP). According to the system, the high specific surface area, the ordered porous structure and the rich surface amino active sites of a covalent organic framework (COF) material are utilized, and the excellent conductivity and biocompatibility of gold nanoparticles (Au NPs) are combined, so that the bifunctional signal probe COF (at) Au (at) MBApt is constructed. The probe can simultaneously load a large number of signal molecule methylene blue (MB) and is coupled with a targeting aptamer (Apt), so that high-capacity labeling and specific recognition of a target marker are realized.

The invention provides a molecular classifier for detecting gastric cancer miRNA. A preparation method of the molecular classifier comprises the following steps: depositing a PABA modification layer on the surface of a screen-printed electrode; activating carboxyl on the surface of the para aminobenzoic acid modification layer by adopting an EDC / NHS composite system; carrying out amination modification on an MXene material, and coating the surface of the activated para aminobenzoic acid modification layer with the modified MXene material to form an MXene functional layer; depositing gold nanoparticles on the surface of the MXene functional layer to obtain an electrochemical activity sensing interface; a signal reporter molecule is combined with an aptamer probe, the signal reporter molecule is a DNA tetrahedron-methylene blue complex, and the combined complex is dropwise added on the surface of an electrochemical activity sensing interface, so that the aptamer probe is combined on the surface of the electrochemical activity sensing interface. The molecular classifier can be used for preparing gastric cancer miRNA detection products, and the problems that in the prior art, a classifier is high in complexity and high in signal leakage risk are solved.

The present application relates to a kind of functional materials for organic dye adsorption and degradation and its preparation method and application, belong to pollutant degradation field.The present application uses one-step hydrothermal synthesis method, with anhydrous ferric chloride, tin chloride tetrahydrate and sodium hydroxide as raw material, successfully constructs the Fe (Sn (OH) 6) / FeSnO (OH) 5 heterojunction composite material with close interface combination.The material can realize the efficient removal of typical organic pollutants methylene blue at normal temperature and pressure, and the degradation rate can reach more than 90% in a short time, showing excellent adsorption and degradation performance.The present application has simple process, mild reaction condition, low raw material cost, avoids complex post-processing process, has good scalability and application potential.The material can provide an efficient, economical technical solution for the treatment of refractory organic pollutants in water, has significant environmental benefits and practical application value.

This invention discloses a quercetin-methylene blue composite photocatalyst, its preparation method, and its application, relating to the field of photocatalyst preparation technology. The method includes the following steps: S1: Quercetin and methylene blue are placed in a beaker, deionized water is added, followed by H2SO4 and anhydrous ethanol, and the mixture is stirred to obtain a mixed solution; S2: The mixed solution is transferred to a hydrothermal reactor with a polytetrafluoroethylene liner for a isothermal reaction; S3: After the isothermal reaction, the mixture is cooled to room temperature, the reaction product is washed with deionized water, and then dried and ground into powder to obtain the final product. The photocatalyst of this invention utilizes the double hydrogen bond between the hydroxyl groups of quercetin and the sulfonic acid and amino groups of methylene blue to construct a stable composite structure, significantly enhancing its photocatalytic activity. Under visible light irradiation, the prepared photocatalyst exhibits excellent depolymerization performance in lignite, efficiently generating humic acid and fulvic acid rich in hydroxyl and amino groups.

The invention relates to the field of biotechnology and dead / living cell detection, in particular to a method for rapidly determining the activity of biological membrane cells in immobilized fermentation. According to the method, a methylene blue and trisodium citrate synergistic chromosome system is optimized, a biofilm EPS matrix is synergistically penetrated by virtue of the dispersion effect of trisodium citrate and the reduction characteristic of methylene blue, and a spectrophotometric method is combined to detect the OD value of a staining solution instead of stained cells, so that the efficient quantitative analysis of the ratio of dead cells to living cells of the immobilized biofilm is realized. The method can complete detection within 200 s, is simple and convenient to operate and low in cost, does not need complex equipment, and is remarkably superior to a traditional fluorescent staining method.

The application belongs to the technical field of biosensing detection, and particularly relates to a preparation method of a photoelectrochemical sensor based on a methylene blue composite material loaded by Ni-MOF and application thereof. The application introduces a green Ni-MOF to fix a photosensitive molecule methylene blue, and SA-ALP catalyzes an electron donor ascorbic acid (AA) generated by ascorbic acid phosphate, thereby constructing a spatially confined aptamer sensor of the methylene blue composite material loaded by Ni-MOF, realizing the improvement of the sensitivity of the detection of MC-LR, and the sensor has good selectivity and stability. The spatially confined photoelectrochemical aptamer sensor developed by the application can realize the rapid, high-selectivity and sensitive analysis of the detection of MC-LR by adjusting the content of AA, and a good recovery rate is obtained.

This invention relates to the field of biomedical technology, specifically to an antifungal nanoparticle, a combination drug, and its application. The antifungal nanoparticle is prepared by covalently linking the aptamer AU1 to drug-loaded silk fibroin nanoparticles via amide bonds. The mass ratio of the drug-loaded silk fibroin nanoparticles to the aptamer AU1 is 20:0.5~2.0. The antifungal nanoparticles of this invention are prepared by a desolvation method using voriconazole-loaded silk fibroin nanoparticles, and the targeting aptamer AU1 is attached to their surface using a carbodiimide chemical cross-linking method. This allows for specific recognition of Candida albicans, increasing drug accumulation at the infection site. Further combination with methylene blue-mediated photodynamic therapy can disrupt biofilm structures, enhance nanoparticle penetration, achieve synergistic bactericidal activity, and reduce systemic toxicity, providing a novel, highly effective, and low-toxicity treatment strategy for fungal biofilm infections.

The preparation method comprises the following steps: adding ammonium citrate as a reducing agent, polyvinylpyrrolidone as a dispersing agent and carboxymethyl cellulose as an adhesive, carrying out hydrothermal reduction on graphene to self-assemble gel, adding ultrasonic-treated multi-walled carbon nanotubes to prepare a multi-walled carbon nanotube / graphene suspension, and finally, carrying out freeze drying to prepare the aerogel. The multi-walled carbon nanotube / graphene composite aerogel prepared through freeze drying in short hydrothermal time and large-scale production has the advantages of ultralight weight, low density and high methylene blue dye pollutant adsorption performance.

The application relates to the technical field of detection, in particular to a general foodborne pathogenic bacteria detection method suitable for a long-distance sailing environment. UiO-66 is used to enrich methylene blue, and is combined with target bacteria aptamer to obtain UiO-66 / MB / Aptamer which simultaneously serves as a signal amplification and recognition probe. A membrane separation method is used to filter out the probes not combined with the bacteria. The electrochemical signal of methylene blue is detected by using a differential pulse voltammetry method, a corresponding linear relationship is constructed, and the concentration of the bacteria is quantitatively detected. The application not only guarantees the sensitivity, detection time, portability and other characteristics of the detection of foodborne pathogenic bacteria, but also provides a general detection method for detecting various target bacteria. Through the reproducibility and stability test under the high-temperature, high-humidity and high-salt environment, it is proved that the application can realize the rapid, sensitive and stable detection of foodborne pathogenic bacteria in a complex long-distance sailing environment.

The invention discloses a calibration method of a nucleic acid quantitative fluorometer, which adopts a methylene blue aqueous solution with stable chemical property as a standard substance to replace a biological nucleic acid reagent, and verifies the optical performance of the nucleic acid quantitative fluorometer by constructing a linear response relationship of a chemical standard substance. The method comprises the following steps: preparing a methylene blue mother solution and a gradient calibration solution, and carrying out light-shielding standing and thermodynamic stabilization treatment; collecting a fluorescence signal by using a nucleic acid quantitative fluorometer to construct a standard curve; selecting a verification sample for reverse measurement, and calculating an indication error, a channel range and measurement repeatability; and finally, judging whether the calibration result is qualified or not through hierarchical logic including linear validity verification and metrological characteristic subentry verification. The problems that an existing RNA kit is high in calibration cost, poor in linearity and unstable in result are solved, and the method has the advantages of being low in cost, easy and convenient to operate, high in result confidence coefficient and the like.

This utility model relates to the field of anti-counterfeiting label technology and discloses a double-sided printed anti-counterfeiting label, including a substrate isolation layer and a protective film installed on the substrate isolation layer, a second label layer, a first label layer, and a main anti-counterfeiting layer. Through the interaction of the brittle film layer, the cavity, and the microcapsule dye inside the cavity, when the customer completes the anti-counterfeiting code verification, they can directly press and break the brittle film layer, and then directly apply force to the microcapsule dye to break it, allowing the methylene blue dye inside to cover the anti-counterfeiting layer, destroying its function and making it unusable. The structure is simple, easy to operate, and highly practical.

The invention discloses a simple preparation and high-efficiency recovery method of polymethylene blue based on composite printing and dyeing wastewater, which realizes high-efficiency recovery of polymethylene blue in composite printing and dyeing wastewater through a multi-stage grid, an adjusting tank, a reaction tank, a sedimentation tank and a dehydration room. Adjusting the composite printing and dyeing wastewater in the adjusting tank to be acidic, reacting the composite printing and dyeing wastewater with peroxydisulfate in a reaction tank, performing high-speed stirring, low-speed stirring and standing separation, decolorizing liquid-phase methylene blue, and depositing solid-phase polymethylene blue; under the action of the sedimentation tank, solid-phase polymethylene blue forms a filter cake, and the filter cake can be stored in the shade for later use after being subjected to vacuum drying. Compared with other dye recovery methods, the method has the advantages of simplicity in operation, low cost, safety, high efficiency, no secondary pollution, wide applicable concentration range and the like, realizes specific recovery of polymethylene blue in the composite printing and dyeing wastewater, and has wide engineering application prospects.

This invention discloses a miR-141 / near-infrared light dual-response synergistic drug delivery system, relating to the fields of biomedicine and nanomedicine. The system uses DNA-modified gold nanocages as carriers, loading doxorubicin (a chemotherapy drug) and methylene blue (a photosensitizer). H-type DNA nanolocks formed by thiol-modified A and B chains achieve specific recognition of miR-141 and dual response to near-infrared light. miR-141 binding triggers initial unwinding of the nanolocks, followed by complete unwinding and drug release via 808nm near-infrared light irradiation (photothermal effect). A 650nm laser excites methylene blue to generate reactive oxygen species, simultaneously completing quantitative detection of miR-141 (fluorescence / SERS signal complementarity) and synergistic treatment in three modes: chemotherapy + photothermal + photodynamic therapy. In vitro experiments show a miR-141 detection sensitivity of 5.0 × 10⁻⁶. ‑12 M, with a dual-response drug release rate exceeding 60%; in vivo experiments have demonstrated a tumor inhibition rate exceeding 70% and good biocompatibility. This system solves the problems of single-response and separation of diagnosis and treatment in existing delivery systems, and is suitable for precision treatment of miR-141 positive solid tumors such as MCF-7.

The application discloses a dopamine in-situ growth ZIF-8 dye / salt separation loose nanofiltration membrane and a preparation method thereof, and comprises the following steps: preparing a Tris buffer solution by taking a Tris buffer solution as a solvent and an alcohol-water mixed solution; preparing a precursor solution by adding dopamine, polyethyleneimine and a metal ion compound into the buffer solution; depositing the precursor solution on a polysulfone membrane surface; dissolving 2-methylimidazole in the alcohol-water mixed solution and then depositing the 2-methylimidazole on the polysulfone membrane surface; pouring off the excess solution, drying the membrane and then storing the membrane in deionized water to obtain a filter membrane. The nanofiltration membrane prepared by the application can intercept dyes and almost completely pass bivalent inorganic salts, and can fully separate a mixed solution of Congo red, methyl blue and sodium sulfate. Meanwhile, the application is simple in operation, can be popularized to various material substrates, and has great application value and development prospect.

The invention provides a BiPO4 / CdIn2S4 composite photocatalyst, a preparation method thereof and application of the BiPO4 / CdIn2S4 composite photocatalyst in photocatalytic degradation of methylene blue, and relates to the technical field of environmental catalytic materials.CdIn2S4 (indium cadmium sulfide) microspheres and rod-like BiPO4 (bismuth phosphate) are prepared through a hydrothermal method, and then a BiPO4 / CdIn2S4 heterojunction is constructed through a mechanical mixing method; wherein the optimal molar ratio of BiPO4 to CdIn2S4 is 1: 5 (marked as BC5). According to the composite photocatalyst disclosed by the invention, through the heterostructure design, the material contact area is obviously increased, the photo-induced electron migration path is shortened, the photo-induced electron-hole pair compounding is effectively inhibited, and the visible light absorption range is widened; under the optimal condition, the MB degradation rate within 180 min reaches 99.31%, the degradation rate is only reduced by 6.09% after five times of recycling, and the stability is excellent. The preparation process is simple and convenient, the cost is low, the methylene blue dye wastewater can be efficiently treated, and the industrial application prospect is wide.

The invention discloses a split type magnetic attraction bipolar electrochemical luminescence sensor, a detection platform and application of the split type magnetic attraction bipolar electrochemical luminescence sensor. According to the invention, a detection chamber and a signal chamber which are physically separated are constructed, and magnetic control capture and signal transduction of target molecules are realized by combining a magnetic response nickel-coated neodymium iron boron (Ni-coated NdFeB) closed bipolar electrode (mag-BPE); meanwhile, mesoporous silica nanoparticles (MBatMSNs) loaded with methylene blue are used as an electrochemical marker, and the signal response intensity is remarkably improved through the electric neutrality principle of BPE. The invention not only provides a high-sensitivity, economical and simple method for detecting the target object, but also provides a technical reference for the development of a multi-index joint detection platform through the modular design and a universal detection interface, and shows a potential application value in the field of instant diagnosis.

This invention discloses a polyacrylamide aerogel supported on a high-entropy spinel ferrite catalyst, its preparation method, and its applications. This invention uses a hydrothermal method to prepare Cu... 1 / 2 Zn 1 / 2 Fe 2 / 3 Co 2 / 3Ni 2 / 3 O4 high-entropy ferrite catalyst (HEO) was supported on polyacrylamide aerogel to form a composite catalyst (PAM@HEO). The performance of this catalyst in photocatalytic degradation of dyes was evaluated using simulated visible light, and the reaction mechanism of the most effective photocatalytic degradation of methylene blue was investigated. This invention utilizes the synergistic catalytic effect of ferrite and polymer aerogel to effectively promote photocatalytic reactions, thereby achieving environmentally friendly pollutant degradation. The production process is simple, does not introduce various toxic or harmful organic substances, is non-toxic and harmless during use, has a catalyst recovery efficiency exceeding 95%, and features significant degradation effects without generating secondary pollution. The preparation method only involves processes such as dissolution, hydrothermal treatment, filtration, and calcination, making it extremely suitable for industrial-scale mass production.

The application belongs to the technical field of medical material preparation, and particularly relates to a composite material based on photodynamic therapy of periodontitis and a preparation method and application thereof. The method disperses sodium dodecyl benzene sulfonate, tin chloride pentahydrate and L-cysteine in a mixed solution of water and ethylene glycol, uniformly stirs, and obtains nanosheets through hydrothermal reaction; then the nanosheets are mixed with mercapto-polyethylene glycol through ultrasonic, to obtain tin disulfide nanosheets; a germanium oxide solution, a chromium nitrate nonahydrate solution, zinc nitrate hexahydrate, hydrated gallium nitrate and water are mixed, stirred in the dark, ammonia water is added and uniformly stirred, and then hydrothermal reaction is carried out, to obtain long-light-emitting nanoparticles; finally, the tin disulfide nanosheets and the long-light-emitting nanoparticles are dissolved in water, stirred in the dark, a methylene blue solution is added and continuously stirred in the dark, and the composite material based on photodynamic therapy of periodontitis is obtained. The composite material based on photodynamic therapy of periodontitis prepared by the method can effectively improve the curative effect of photodynamic therapy in the treatment of periodontitis.

The invention discloses an electrochemical sensor for IBD protein marker detection as well as a preparation method and application of the electrochemical sensor. The electrochemical sensor is composed of a semi-cured PDMS flexible substrate in which CNT is embedded, deposited gold nanoparticles and a nucleic acid sensing layer, the nucleic acid sensing layer comprises a tumor necrosis factor-alpha sensor, an interleukin 6 sensor, an interleukin 8 sensor, a transforming growth factor beta1 sensor, a reference electrode and a counter electrode; the tumor necrosis factor-alpha sensor, the interleukin 6 sensor, the interleukin 8 sensor and the transforming growth factor beta1 sensor all adopt aptamer single chains modified by signal molecule methylene blue, and correspondingly adopt nucleotide sequences shown in SEQ ID NO.1-4. Matching and excellent biocompatibility of the sensor and human tissue modulus can be improved, and accurate detection of low-concentration biomarkers and stable signal output of the sensor under the strain working condition are achieved.

The invention provides a photo-initiation system and application thereof. The photo-initiation system comprises a photosensitizer and a co-initiator, wherein the photosensitizer is obtained by modifying a basic dye by using a modifier; the modifying agent is prepared from a C6-C20 alkyl sodium sulfosuccinate compound; the basic dye comprises at least one of ethyl violet, navy T, basic violet 7, basic fuchsin, methylene blue, neomethylene blue and azure A. The photosensitizer in the photo-initiation system provided by the invention is obtained by modifying the basic dye by using the modifier, and the types of the modifier and the basic dye are limited, so that when the photo-initiation system is used for preparing the holographic grating, the photosensitizer is easily dispersed in a solution, is not easy to form a precipitate and is more easily bleached by visible light; therefore, the diffraction efficiency, the refractive index modulation degree and the transmittance of the holographic grating can be improved.

The invention discloses a BiOI-based composite photocatalyst and a method for photocatalytic degradation of methylene blue, and relates to the technical field of methylene blue degradation. The method comprises the following steps: firstly, adjusting the pH value of methylene blue wastewater to be less than or equal to 7.0, then adding a BiOI-based composite photocatalyst and H2O2 into the methylene blue wastewater, and carrying out light treatment for not less than 40 minutes to finish photocatalytic degradation of methylene blue. The research of the invention finds for the first time that the BiOI-based composite photocatalyst not only has certain activity of photocatalysis of methylene blue, but also can be combined with hydrogen peroxide to improve the catalytic activity of hydrogen peroxide; compared with single application, the combined use of the two has obviously higher degradation efficiency on methylene blue in wastewater, and the highest degradation efficiency is up to 98.0%.

The invention belongs to the technical field of geotechnical engineering physical model tests, and discloses a tunnel lining physical model crack identification method based on a double-oxidation-reduction indicator, which comprises the following steps: weighing raw materials of similar materials, uniformly stirring, adding the double-oxidation-reduction indicator, continuously stirring, and pouring into a lining test piece; the double-oxidation-reduction indicator is methylene blue and dichlorophenol indophenol, the methylene blue is fixed on the sand surface in the similar material through physical adsorption, and the dichlorophenol indophenol is dissolved in water in the similar material and dispersed in the matrix; loading until the lining cracks through a model testing machine, synchronously recording a crack propagation process by using a high-speed camera, and collecting an image; segmenting a crack region by using a threshold segmentation method, and calculating an average RGB value of a crack center pixel; and substituting into a crack width-color parameter regression formula to calculate the crack width. On the premise of not changing a similar material matrix, uniform dispersion and stable color development of the indicator are realized, so that the reliability of a physical test result is ensured.

The invention discloses an iron-doped carbon dot nano-enzyme assisted ratio type electrochemical aptamer sensor, a preparation method and application, and belongs to the field of sensors. The method comprises the following steps: (1) preparing a molybdenum disulfide-gold nanoparticle-methylene blue composite material modified electrode; (2) preparing a MoS2 / Au / MB / cDNA modified electrode; (3) preparing the iron-doped carbon dot nano enzyme; (4) preparing the iron-doped carbon dots modified by the acetamiprid aptamer; and (5) preparing the MoS2 / Au / MB / cDNA (at) Apt / Fe-CDs modified electrode. The preparation method disclosed by the invention is low in cost, simple in experimental operation and easy in reaction condition control. The iron-doped carbon dot nano enzyme-assisted ratio-type electrochemical aptamer sensor prepared by the invention can be used as a working electrode for quantitative analysis and detection of acetamiprid, and has good stability, high sensitivity, high specificity and high accuracy in acetamiprid detection.

A system for the synthesis and characterization of luminescent copper oxide nanoparticles, comprising: a) an extract preparation plant configured to produce an extract from white garland leaves by boiling cut leaves in double distilled water and subsequently filtering the mixture; b) a synthesis unit configured to mix a copper sulfate solution with the extract from white garland leaves and to obtain a black colored solid under stirring conditions; c) a centrifuge configured to separate the black-colored solid from the mixture; d) a combustion unit configured to subject the separated solid to heat treatment in a muffle furnace to obtain copper oxide nanoparticles; e) a characterization unit for the analysis of the copper oxide nanoparticles, wherein the characterization unit includes: UV-Vis spectrophotometer, X-ray diffractometer, photoluminescence spectrometer, etc. f) a photocatalytic degradation unit configured to enable the complete removal of industrial dyes (methylene blue) and g) an antimicrobial test unit for evaluating the antibacterial and antifungal activity of the copper oxide nanoparticles.

The application discloses a method for determining the methylene blue adsorption capacity of montmorillonite, which comprises the following steps: S1, montmorillonite dispersion, stirring and dispersing the dried montmorillonite after wetting with water, then adding a composite dispersing agent and continuing to stir, heating to micro-boiling, cooling to obtain the dispersed montmorillonite mixture; S2, methylene blue adsorption, adding excess methylene blue solution dropwise to the dispersed montmorillonite mixture under stirring and stirring to make the reaction complete, then performing suction filtration to obtain the precipitate, and transferring the precipitate to a glass sand crucible; S3, drying to constant weight, drying the glass sand crucible containing the precipitate to constant weight, at the same time, performing a blank experiment, and the treatment is the same except that no sample is added, finally, the methylene blue adsorption capacity of the montmorillonite can be obtained by substituting into the formula. The application is a gravimetric method, which is not affected by personal subjective consciousness, and the obtained determination result is good in repeatability and high in accuracy.