Method for detecting blood homocysteine and kit

A homocysteine, detection kit technology, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, material excitation analysis, etc., can solve the problem of high sensitivity, high price, color development Reaction and other problems, to achieve the effects of good reagent stability, sensitive method and high repeatability
CN102901720AInactive Publication Date: 2013-01-30INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
2 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
Publication Date
2013-01-30
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The utility model discloses a method for detecting blood homocysteine and a kit in the field of biological detection technologies. The kit comprises a kit body, a 96-pore plate, HCY standard substance, HCY specific convertase, a reducing agent, a buffer solution, color developing liquid A and color developing liquid B, wherein the 96-pore plate, the HCY standard substance, the HCY specific convertase, the reducing agent, the buffer solution, the color developing liquid A and the color developing liquid B are arranged in the kit body. The kit utilizes HCY in rHCYase decomposed blood to produce H2S, produced H2S is reacted with dimethyl-p-toluidine to produce methyl blue, and the HCY content in the blood is reflected through the methyl blue generating amount. The kit is simple in operation, a reagent is good in stability and high in repeatability, and the method is flexible and suitable for quick clinical HCY detection of laboratories.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a blood homocysteine ​​detection method and a kit. Background technique

[0002] Studies in the past ten years have confirmed that a high concentration of homocysteine ​​(Homocysteine, HCY) in the blood is an independent risk factor for coronary artery disease, cerebrovascular and peripheral vascular diseases. HCY is a sulfur-containing amino acid produced by demethylation of methionine. It is metabolized in the body through two pathways: remethylation and transsulfurization: first, under the catalysis of methionine synthase, vitamin B12 is used as the The coenzyme uses N5-methyltetrahydrofolate as the methyl donor, and HCY is methylated to generate methionine. The key enzyme in this process is methylenetetrahydrofolate reductase (MTHFR) with vitamin B2 as a coenzyme. This enzyme reduces N5, N10-methylenetetrahydrofolate to N5-methyltetrahydrofolate, whi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More