151 results about "Color reaction" patented technology

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention discloses a dynamic leaching-spectrophotometric detection method for the release rate of nutrients in a slow-release fertilizer, and relates to the field of agrochemical analys.The detection method comprises the following steps that S1, a slow-release fertilizer sample is placed in a leaching column to be leached, and leachate is collected; s2, sequentially adding a masking agent solution and a chromogenic reagent into the leachate, and carrying out chromogenic reaction; s3, pumping the solution into a double-optical-path flow cell, and measuring absorbance values under a target nutrient characteristic absorption wavelength and a background interference characteristic wavelength; s4, calculating the corrected net absorbance; and S5, calculating the nutrient concentration, and drawing a nutrient release kinetic curve. By adopting a dual-wavelength synchronous detection and real-time dynamic subtraction correction algorithm, the method can eliminate system errors of time-varying background chromaticity, so that accurate and reliable absorbance readings can be obtained at the initial stage of nutrient release even if the nutrient concentration in the leachate is very low, thereby ensuring the authenticity of the initial stage of a release curve and improving the accuracy of nutrient release. And initial release kinetic parameters are accurately captured.

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The invention discloses a red soil dry land soil microbial diversity detection method based on multicolor developing test paper, and belongs to the technical field of soil environment microbial detection. According to the method, test paper loaded with different color developing agents is designed, a specific color developing reaction is triggered by combining microbial metabolic activity, and color developing characteristics are converted into parameters such as microbial diversity indexes and dominant species proportions by utilizing an image analysis model. The detection device comprises an image acquisition module and a data analysis module, and quantitative association of color development features and diversity is realized through a machine learning model. Compared with the prior art, the method has the advantages that the cost is extremely low, the detection time is shortened to be within 30 minutes, the method is suitable for field real-time monitoring of the red soil dry land, an efficient, low-cost and portable detection tool is provided for soil health management, and the method is particularly suitable for soil health assessment of resource-limited areas.

The utility model provides a portable chromogenic reaction device, and relates to the technical field of detection. The portable chromogenic reaction device comprises a base, a first reaction cavity and a second reaction cavity, wherein the base is provided with an indentation line extending in the width direction of the base, the upper surface of the base is covered with a removable sealing layer, and the sealing layer is used for achieving airtight storage of the device; the first reaction cavity is arranged on the base in a protruding manner, and a pre-reaction reagent is pre-arranged in the first reaction cavity; the second reaction cavity is arranged on the base in a protruding mode, the first reaction cavity and the second reaction cavity are located on the same side of the base, and a color developing element is arranged in the second reaction cavity in advance. The first reaction cavity and the second reaction cavity are arranged on the two sides of the indentation line respectively, and when the base is folded along the indentation line, the second reaction cavity can be pressed to deform, so that the color developing element in the second reaction cavity is mixed with the liquid in the first reaction cavity. The device is portable, and the detection process is simplified at least to a certain extent.

The application provides a human Helicobacter pylori detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology, amplifies the genomic fragment containing the four-strand DNA sequence in the human Helicobacter pylori genome, and realizes rapid and visual detection of the human Helicobacter pylori by adding hematin and oxidoreductase substrate for color reaction.

The application discloses a cyanide-containing slag tailing pond groundwater environment risk early warning system, a groundwater monitoring well subsystem comprises a plurality of groundwater monitoring wells; a timing sampling control subsystem is used for controlling the pumping time, flow and frequency of a water pump in each groundwater monitoring well; a color reaction subsystem comprises a color developing device and is used for monitoring the color developing reaction of the groundwater sample; a color sensing subsystem comprises a light source and a color sensor, the light source is irradiated towards the color developing device, and the color sensor is used for collecting the emergent light signal of the color developing device after the color developing reaction; a central control platform comprises an early warning subsystem and an emergency disposal response subsystem, the early warning subsystem receives the color sensor signal and divides the risk grade according to the obtained signal; the emergency disposal response subsystem executes corresponding detection and repair responses according to the risk grade made by the early warning subsystem; the application realizes the rapid detection of the cyanide content in the groundwater around the cyanide-containing slag tailing pond and the feedback and early warning of the environmental pollution risk grade.

This utility model discloses a portable rapid detector for pesticide residues in food, comprising: a sample container, a tube head integrally installed on the top of the sample container, and a top cover installed above the tube head. A threaded sleeve is fixedly connected to the bottom surface of the top cover, and a docking hole is hollowed out in the middle of the top cover. A cap is installed on the top of the top cover by connecting bolts, and a reagent tube is installed inside the docking hole. The reagent tube is continuously fed into the sample container through the threaded connection structure between the top cover and the sample container. A rubber stopper is provided at the bottom of the reagent tube. Inserting the tube will puncture the rubber stopper at the bottom of the reagent tube. A through hole is provided on the surface of the inserting tube. After the inserting tube is inserted into the reagent tube, the reagent will flow into the sample container through the through hole on the surface of the inserting tube. After shaking the sample container, the reagent comes into contact with the agricultural product sample. Pesticide residues will cause the reagent to produce a color reaction. The color reaction is observed through the observation window on the surface of the sample container. The presence of pesticide residues can be quickly determined after the color reaction occurs.

The present disclosure relates to a diagnostic cartridge for immunodiagnosis, and a reader and a diagnostic system using the same. A diagnostic cartridge, including the diagnostic pad and the color reaction pad, is provided to detect a color reaction and a fluorescence reaction through the single diagnostic cartridge.

To provide a water-based ink composition for a ballpoint pen, which is excellent in dry-up resistance and can form clear handwriting, and to provide a ballpoint pen containing the ink composition.SOLUTION: An aqueous ink composition for a ballpoint pen, comprising: a colorant; an alkyl polyglucoside having a C8 to C14 aliphatic-hydrocarbon group; a shear-thinning agent; and water. The colorant contains a reversible thermochromic microcapsule pigment containing a reversible thermochromic composition comprising at least (a) an electron-donating color-developing organic compound, (b) an electron-accepting compound, and (c) a reaction medium for determining the temperature at which the color development reaction of the components (a) and (b) occurs.SELECTED DRAWING: None

The application belongs to the technical field of circuit board detection, and particularly relates to a circuit board cavity residual detection, identification and analysis method. The method first locates a suspected residual area according to the gray difference of an X-ray image and generates a spatial coordinate set thereof, then, combines chemical color reaction intensity and X-ray profile characteristics to screen out a to-be-analyzed area set, further divides the area set into a plurality of subsets according to spatial diffusion characteristics, obtains element and functional group data of each area through energy spectrum and spectrum analysis, analyzes a candidate residual class thereof, dynamically adjusts subset division based on consistency of the candidate class in the subset, until the consistency requirement is met, and finally outputs a specific residual class for each area according to the division result. According to the spatial proximity and profile characteristics, the application realizes systematic differentiation and classification of diffusion-type residues and adhering-type residues, and improves the accuracy of class determination.

The invention relates to a color developing solution for detecting iron metal foreign matters in a lithium battery production process and a detection method, and the color developing solution comprises the following components in parts by volume: 3-4 parts of an acid solution, 1-2 parts of an oxidizing agent and 3-4 parts of a thiocyanate solution. The color developing solution is provided for detection of the iron metal foreign matter in the lithium battery production process, the iron metal foreign matter in the lithium battery production process is rapidly and qualitatively detected by utilizing a color developing reaction of ferric ions and thiocyanate ions to generate a blood red complex iron thiocyanate, and the detection sensitivity, the detection efficiency and the speed are high; the reagent of the color developing solution is nontoxic and harmless, the cost is low, the color developing solution is particularly suitable for large-batch detection on a mass production line, the detection efficiency of the production line is greatly improved, and meanwhile, high detection sensitivity is taken into account.

This invention discloses a method and application for constructing a dual-mode sensor for detecting diphenyl phosphate using smartphone-assisted surface molecular imprinting, belonging to the fields of biochemical analysis, functional nanomaterials, and life science technology. In the synthesis process, the solubility, selectivity, and photoinitiation efficiency of the target analyte during polymerization are optimized, and molecularly imprinted nanozymes (MIPs) for diphenyl phosphate are rapidly prepared using molecular imprinting technology. In the detection process, the peroxidase-like activity of Fe,N-CDs catalyzes the colorimetric reaction between H₂O₂ and 3,3,5,5-tetramethylbenzidine (TMB), and the internal filtering effect of oxTMB suppresses the fluorescence signal. Combined with a smartphone, dual-signal cross-validation is achieved, constructing a colorimetric-fluorescence sensor for detecting diphenyl phosphate. This sensor exhibits specific recognition selectivity and binding affinity for diphenyl phosphate, is simple to operate, and low in cost. It can achieve sensitive detection of diphenyl phosphate over a wide linear range, with a detection limit as low as 16 pg / mL, making it suitable for on-site analysis of trace DPH in complex aquatic environments.

The application provides a double-layer structure colorimetric test paper for medical equipment occult blood detection and a preparation method and application thereof, and belongs to the technical field of hospital infection control and cleaning quality monitoring. The colorimetric test paper adopts a double-layer structure design: the bottom layer is obtained by immersing qualitative filter paper in immersion liquid A and drying, and is used for storing oxidant stably; and the upper layer is formed by uniformly coating coating liquid B on the surface of the bottom layer, and is used as a chromogenic substrate layer. When in use, hemoglobin remaining on the surface of the medical equipment can catalyze H2O2 in the bottom layer to oxidize TMB in the upper layer, a blue color reaction is generated, and naked-eye visual judgment is realized. The test paper physically separates the oxidant and the substrate, significantly improves the storage stability and detection sensitivity, and has the advantages of simple preparation process, low cost, convenient operation, and the like. The test paper is suitable for on-site rapid evaluation of cleaning effects of surgical instruments, endoscopes and the like, and has good clinical application prospect and popularization value.

The application provides a precise positioning method for sewer pipeline leakage based on a concentration gradient color development principle, and comprises the following steps: S1, fixing a color development tape connected with a deployer in a manhole upstream of a pipeline, allowing the deployer to advance spirally along a central axis direction of the pipeline, and continuously releasing the color development tape in the process, so that the color development tape is radially and tightly pressed and fixedly attached to an inner wall detection area of the pipeline, and is arranged along a spiral track in the central axis direction of the pipeline until the deployer reaches a manhole downstream of the pipeline; S2, continuously injecting a tracer upstream of the pipeline; S3, activating a color development reaction of the color development tape under a sewage environment, so as to form a color development intensity gradient corresponding to a pipeline leakage point in a length direction of the color development tape, and the color development intensity gradient is positively correlated with a pipeline leakage flow; and S4, continuously scanning the color development tape by using a colorimetric analyzer method to obtain color development intensity, and determining the pipeline leakage point and leakage intensity according to the color development intensity and color development tape position information.

The invention discloses an ozone concentration online monitor based on an electrochemical sensor, and belongs to the technical field of environment monitoring. The instrument comprises a shell, a display assembly, an air inlet window, an air outlet window, a sampling part, a limiting part and a detection part, the sampling component comprises a lifting base, a lifting column, a lifting plate, a rotating column, a rotating bracket and a macro camera, and is used for identifying the concentration of interference gas such as sulfur dioxide or nitrogen oxide through a chromogenic reaction; the limiting component comprises a supporting bracket, a pressing bolt, a pressing piece, a semi-permeable net and an air blower and is responsible for air filtration and pretreatment; the detection component comprises a detection sensor and a detection probe and measures ozone concentration based on an electrochemical principle. According to the instrument, through the sequential detection process, after air suction, filtering and interference recognition are conducted firstly, then ozone is corrected and measured, and the problem of inaccurate detection caused by cross interference in the prior art is solved. The device has the beneficial effects of high precision, automation, quick response and easy maintenance, and is suitable for continuous monitoring of air quality.

The present application relates to the field of agricultural chemical analysis, and particularly relates to a method for grading extraction and fluorescent labeling quantitative detection of effective phosphorus forms in organic fertilizer, first, water-soluble phosphorus is extracted by oscillation with deionized water; then, the filter residue is treated with an ammonium citrate-EDTA complexing extraction liquid to obtain citric-soluble phosphorus; finally, the residue is dissolved with a mixed acid extraction liquid to obtain insoluble phosphorus, three kinds of extraction liquids are taken respectively, color developing reagent of ammonium molybdate, ascorbic acid reducing agent are added in turn to carry out color developing reaction, then a fluorescent enhancer is added to amplify the signal, the fluorescence intensity is measured, and the contents of water-soluble phosphorus, citric-soluble phosphorus and insoluble phosphorus are accurately calculated according to a standard curve, a water-ammonium citrate / EDTA-mixed acid three-stage gradient extraction system is adopted to realize efficient separation and extraction of water-soluble phosphorus, citric-soluble phosphorus and insoluble phosphorus in the organic fertilizer, especially, the synergistic complexing system of ammonium citrate and EDTA is introduced in the second-stage extraction, thereby improving the extraction efficiency of citric-soluble phosphorus.

The invention discloses an unsymmetrical dimethylhydrazine detection method based on a 2, 4-dinitrobenzaldehyde chromogenic reaction, a reagent and a sensing film, and belongs to the field of dangerous chemical detection, the embodiment of the invention further provides the unsymmetrical dimethylhydrazine detection method based on the 2, 4-dinitrobenzaldehyde chromogenic reaction, a to-be-detected sample is in contact with an absorption liquid, and the absorption liquid is detected through the unsymmetrical dimethylhydrazine detection method based on the 2, 4-dinitrobenzaldehyde chromogenic reaction. Observing whether a color change from yellow to brownish red appears; the absorption liquid comprises 2, 4-dinitrobenzaldehyde, an acidic medium and a solvent. The existence of unsymmetrical dimethylhydrazine is judged by observing whether the color change from yellow to brownish red appears or not and observing the color depth or the size of a color change area. According to the method, the unsymmetrical dimethylhydrazine as low as 1 ppm in a gas phase and as low as 0.1% in a liquid phase can be subjected to rapid and distinct yellow-to-brown yellow chromogenic reaction, and common interference such as 50 ppm ammonia gas can be effectively inhibited in an acidic environment.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

The application discloses a microbial nano-enzyme probe, a method for detecting salbutamol and application, and comprises a salbutamol monoclonal antibody and a signal carrier, wherein the signal carrier is a yeast cell loaded with iridium oxide nanoparticles. The application firstly constructs a probe by using a yeast cell loaded with iridium oxide nanoparticles in the detection of an immunochromatographic test strip, uses [3, 3', 5, 5' tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2)] as a substrate to trigger a color developing reaction, can realize high-sensitivity target detection, and the detection limit is as low as 0.012 ng / mL, which is 55 times higher than the sensitivity of a traditional gold-labeled test strip. In addition, the application has a good application prospect, can provide a universal platform for sensitive, accurate and rapid detection of food-borne stimulants, and can be successfully applied to the detection of salbutamol in pig liver and beef samples.

The invention relates to the technical field of semiconductor manufacturing, and discloses a titanium etching solution preparation process based on a hydrogen peroxide-monoethanolamine system.The titanium etching solution comprises, by mass, 5%-15% of hydrogen peroxide, 5%-15% of monoethanolamine, 5%-15% of sodium hydroxide and the balance water. 5%-10% of monoethanolamine; 2%-5% of tris (hydroxymethyl) aminomethane; 0.5% to 1.5% of 2, 2 '-dipyridyl; and 0.1%-0.5% of a double-effect stabilizing and indicating agent. Trihydroxymethyl aminomethane and 2, 2 '-dipyridyl cooperate to stabilize hydrogen peroxide, and a stable pH buffer system is constructed. The key double-effect stabilizing agent and the indicator (such as salicylic acid) can be subjected to complexing chromogenic reaction when the etching product titanium ions are accumulated to reach a threshold value, so that the self-indication function of solution failure is realized. The invention discloses a preparation process for dropwise adding hydrogen peroxide under cooling and temperature control conditions. The titanium etching solution disclosed by the invention has the beneficial effects of high chemical stability, good Ti / Cu etching selectivity and in-situ failure early warning function.

According to the embodiments, an information processing system (1, 2) comprises: an acquisition unit (101, 301) that acquires an image captured of the color of a color reagent of a color reaction that uses the feces of a subject; and a determination unit (103, 303) that determines intestinal environment information about the state of the intestinal environment of the subject on the basis of the captured image. The color reagent is a reagent that changes color depending on the pH or polarity of a biological sample in the feces. The determination unit (103, 303) determines the concentration or abundance of an intestinal metabolite and at least one of the abundance ratio, abundance, and Shannon index of an intestinal bacterium as the intestinal environment information.

This invention discloses an immunochromatographic method for the simultaneous detection of multiple mycotoxins in grains and oils. The method includes: pulverization and weighing: pulverizing the grain sample and weighing the pulverized sample into a centrifuge tube; adding purified water and a formulation packet, sealing the tube, vigorously shaking at high speed to mix, centrifuging to obtain the supernatant; collecting the supernatant; adding it separately to a reactor; inserting test strips for detecting different mycotoxins into the reactor and waiting for the colorimetric reaction to complete the detection. The formulation packet for the water extraction detection of multiple mycotoxins, based on 100 samples, includes the following components: 7-9 parts bovine serum albumin, 3-5 parts HLB packing material, and 3-5 parts PAS packing material. This invention is based on the principle of traditional chromatographic detection methods, utilizing water extraction by combining different formulation packets with the analytes for extraction, optimizing the experimental steps and reducing the use of organic solvents.

The invention provides a nano-enzyme system based on an Ag-polydopamine nano-microsphere (at) MOF-74 structure as well as a preparation method and application of the nano-enzyme system, and belongs to the technical field of biological medicines. The nano-enzyme system takes a polydopamine microsphere as a core, and an MOF-74 shell layer grows on the surface of the polydopamine microsphere in situ to form a composite microsphere with a core-shell structure; and by utilizing the reduction property of polydopamine, Ag nanoparticles are reduced in situ and uniformly dispersed in the MOF-74 framework, so that the composite nano material with the enzyme-like activity and the photo-thermal function is constructed. The system shows excellent horse radish peroxidase-like activity, and can effectively promote electron transfer between hydrogen peroxide and a substrate and catalyze a chromogenic reaction. Meanwhile, the composite system has good photo-thermal conversion performance, shows a remarkable photo-thermal sterilization effect on gram-negative bacteria and gram-positive bacteria, and can effectively inhibit and destroy the formation of bacterial biofilms. An in-vitro antibacterial experiment result shows that the MOF-74-based nano enzyme system has relatively high antibacterial activity.

The application relates to the field of polyamide material analysis, and discloses application of an indantrione color developing reagent in determination of polyamide hydrolysis products and a method for determining the content of the polyamide hydrolysis products, wherein the indantrione color developing reagent contains an indantrione-ethylene glycol solution and an acetic acid-sodium acetate buffer solution. The application provided in the application adopts ethylene glycol as a single solvent of the indantrione, effectively inhibits oxidative degradation of the indantrione under high-temperature color developing conditions, and enables uniform color development of oligomers. The application does not add antioxidants, avoids interference with color developing reactions, is accurate and reliable in determination, is simple in preparation due to the single solvent, has a long storage time under light shielding at 4 DEG C, and can not only accurately determine monomers of polyamides and dimers to heptamers of the monomers, but also accurately determine various polyamide hydrolysis products, and has a good linear relationship.

The invention discloses a high-throughput food-borne pathogenic bacterium detection method based on a crRNA-DNA compound slow release effect, and belongs to the technical field of biological detection. An isothermal amplification primer and a crRNA-DNA compound are designed for five food-borne pathogenic bacteria, isothermal amplification, a gold nanomaterial and a CRISPR activation detection technology are combined, the CRISPR-Cas12a and the isothermal amplification process are in a buffer system by utilizing the slow release effect of the crRNA-DNA compound, and the detection of the food-borne pathogenic bacteria is realized. And the color development reaction is realized by utilizing the modified SH-DNA gold nanoparticles positioned on the cover. According to the invention, isothermal amplification and CRISPR activation in a detection system are simultaneously carried out by utilizing the slow release effect of crRNA-DNA, the reaction time is shortened, uncovering reaction is not needed, the operation is simple, the sensitivity is high, and the detection specificity is strong.

The invention relates to a method for detecting pesticide based on a ferriporphyrin modified covalent organic framework nano-enzyme sensing array. According to the invention, ferriporphyrin molecules are loaded on a porous and ordered COFs skeleton, so that excellent peroxidase simulated catalytic activity is realized, H2O2 can be catalyzed to be decomposed to generate reactive oxygen free radicals, and TMB, OPD and ABTS chromogenic reactions are catalyzed. Based on the performance, a three-channel colorimetric sensing array is constructed to detect and distinguish various pesticides such as malathion, profenofos, chlorpyrifos, acephate and dimethoate, and the detection limit is as low as 0.341 nM. After different pesticides are adsorbed to active sites, catalytic reaction can be remarkably inhibited, and specific colorimetric fingerprint signals are generated. Colorimetric data is further combined with a machine learning algorithm, high-accuracy classification and recognition of single pesticides and mixtures thereof are achieved by means of a multilayer sensor, a support vector machine, a random forest and other models, the classification accuracy of an MLP model reaches 90%, ROC curve AUC exceeds 0.97, and the reliability of the detection method is proved.

The invention discloses a DAB immune color development kit capable of enhancing color development performance and product stability and application of the DAB immune color development kit, and relates to the technical field of immunohistochemical staining. The DAB immune chromogenic kit provided by the invention comprises a DAB A solution, a DAB B solution and a DAB enhancer, the DAB A liquid comprises hydrochloric acid, 3, 3 '-diaminobenzidine and a specific polymer component; the DAB solution B comprises hydrogen peroxide, disodium ethylene diamine tetraacetate, tris (hydroxymethyl) aminomethane, hydrochloric acid and imidazole. A specific polymer component is introduced to form a compound with DAB, so that the rigidity of a precipitation product is remarkably enhanced, the problems of deformation and collapse caused by water loss in a dry sealing process are effectively solved, and the morphological characteristics of cell nucleuses are completely maintained. By optimizing the pH ratio system of the solution A and the solution B, the sensitivity and the dyeing strength of the chromogenic reaction are optimized, and the chromogenic strength and the sensitivity are further improved in cooperation with the copper sulfate enhancer. The kit is particularly suitable for nuclear antigen labeling items such as Ki-67 and the like, and is suitable for manual and automatic platforms.

In the field of medical supplies and food / drug storage technology, we provide a drug storage device for maintaining nattokinase activity. The solution includes a box body 1 and a screw-fitted box cover 2. The box body contains a main box 11 for storing capsules and a micro-perforated desiccant inner tray chamber 12, which physically isolates the capsules and keeps them fresh. The key feature is the activity detection module 21 integrated into the box cover. This module is equipped with a dedicated capsule detection chamber, and uses a built-in needle sampling mechanism to drip the capsule contents onto a specific colorimetric substrate test strip at the bottom. An optical sensor reads the RGB values ​​of the color reaction, and after processing by an MCU controller, the activity results are output via a display mechanism. This device integrates in situ, rapid, quantitative detection of nattokinase capsule activity with intelligent storage, providing simple operation and intuitive, reliable results. It solves the problems of conventional technology, which make it difficult to easily quantitatively detect nattokinase capsule activity and which are susceptible to moisture inactivation during storage.

The invention provides a resazurin chromogenic stop buffer and application thereof, and belongs to the technical field of biological detection. The resazurin chromogenic stop buffer is a solution containing SDS and a buffer system, and the pH value of the solution is larger than or equal to 8. The invention aims to solve the problem of color fading during the existing resazurin color development, can effectively terminate the color development reaction, and ensures the stability and reliability of the color development result. According to the resazurin color development stop buffer, an environment-friendly formula and a process are adopted, and the burden on the environment is reduced. The technical breakthrough is expected to promote wide application of resazurin chromogenic reaction in the field of biotechnology, a more reliable and environment-friendly tool is provided for researches such as microbiological detection, cell activity detection and enzyme activity determination, and development of related scientific research and industries is further promoted.