757 results about "Color reaction" patented technology

A method for applying gold nanoparticles mimetic enzyme in biological detection adopts gold nanoparticles instead of horse radish peroxidase (HRP) in the biological detection. The detection comprises the following steps: coupling the gold nanoparticles and a specific molecular probe to construct a specific nano-probe; performing specific binding between the nano-probe and the corresponding target molecule to be detected; developing with coloring solution containing peroxide and hydrogen-donating substrate, and measuring absorbance or performing microscopic observation so as to realize the qualitative and quantitative detection of the target molecule. The method belongs to the nanometer material and biomedical nanometer technical field. In the method, the size of the used gold nanoparticles mimetic enzyme is 1-1000nm, the gold nanoparticles mimetic enzyme can imitate HRP to catalyze peroxide and hydrogen-donating substrate to perform a color development reaction, and the enzymatic activity increases with the decrease of the size of the gold nanoparticles. The method of the invention uses the gold nanoparticles to label antibody and other biological molecules to build the similar enzyme labeled antibody and other diagnose preparations, thus having extensive application value.

The present invention discloses a quantum point labeled sandwich immunodetection method and its diagnosis kit. It is a new type sandwich immunodetection method using QDs nano particle as label to make antigen antibody specificity sandwich reaction. It includes the following processes: firstly, directly or indirectly enveloping captured antibody in microwell of polystyrene plate, forming captured antibody-antigen-detection antibody three-layer sandwich luminescent immune complex and fluorescence intensity detection. According to that every QD has narrow and symmetrical fluorescence spectral peak it can select and use quantum point label needle sending different light to simultaneously detect several antigens to be tested in same sample.

The invention discloses a reaction tube used in a loop-mediated isothermal amplification technique, wherein the reaction tube is composed of two parts of a tube body and a tube cover; the lower part of the inner cavity of the tube body is provided with a longitudinally outspread clapboard which separates the inner cavity of the tube body into two cavities of A and B; the cavity A and the cavity B are respectively provided with loop-mediated isothermal amplification reaction working solution or developing solution, the upper layers of the liquid in the two cavities are both sealed up by paraffin wax. Through the design of the clapboard and the paraffin wax, the reaction tube in the invention effectively separates the developing solution and the working solution, thus not only ensuring to relatively maintain complete closure in the storage and transportation process, but also having no need to open the tube cover to realize the developing reaction and greatly reduce the pollution of aerosol. The reaction tube of the invention ensures rapid and efficient detection of loop-mediated isothermal amplification, and has low cost and is beneficial for large-scale promotion.

The invention discloses a colorimetric detection method for glucose. The colorimetric detection method for the glucose comprises the steps of pretreating a sample, adding a buffer solution to dilute the sample, mixing the sample with glucose oxidase, performing reaction to generate hydrogen peroxide, then adding a molybdenum disulfide solution, a developing agent and the buffer solution, and measuring the content of the glucose in the sample by a visual colorimetry method or through an ultraviolet-visible light spectrophotometer after mixing reaction is implemented. According to the colorimetric detection method, glucose oxidase is used for oxidizing the glucose to generate the hydrogen peroxide, and molybdenum disulfide serving as a catalyst is used for catalyzing the hydrogen peroxide to generate color development reaction with the developing agent; the glucose concentration is measured in a semi-quantified manner by the visual colorimetry method or is measured in a quantified manner through the ultraviolet-visible light spectrophotometer. According to the colorimetric detection method for the glucose, the problems of high operation requirement on glucose detection, complicated detection process, high detection cost, long detection time, high background interference and the like in the prior art are solved; the method disclosed by the invention is low in cost and easy to operate; the glucose content of the sample can be quickly detected in a visual manner.

An End of Service Life (ESLI) and Residual Life Indicator (RLI), for gas masks and other filters. The indicator includes a solid conduit with one end closed by a transparent material and the other by a porous material which allows gas to permeate through it. In one implementation of the ESLI, a fine powder that can react chromogenically is placed in the conduit. The powder is free to move in the conduit, but it cannot escape. The porous end of the conduit is inserted into the filter to the desired depth. When a target gas reaches the porous end, it enters the conduit, reacts chromogenically with the powder and changes color. The powder can be viewed through the conduit by tilting the filter to cause the powder to gravitate into the transparent end. A color chart is attached to the filter near the transparent end to facilitate a determination of whether the chromogenic powder has undergone a color change or not. In another implementation of the indicator, the porous material includes a reagent that can react with the target gas and release a reactive gas. The transparent end of the conduit is coated internally with a chromogenic reagent that changes its color when either the target gas or the gas produced in the reaction reaches it. The ESLI/RLI may be inserted into the filter from different directions to accommodate practical filter or mask designs. Two or more ESLI/RLI may be inserted into the filter from different directions to alert the user to different levels of filter consumption. The ESLI/RLI may contain more than one chromophore to alert users of the penetration of different gases or classes of gases.

The invention provides a test strip for detecting heavy metal-chromium (Cr) in water quality and a preparation method thereof, and belongs to the technical field of water quality detection. The test strip sequentially comprises an insulating waterproof substrate, a reaction substrate membrane, a filter layer and a cover plate which are bonded together from the bottom up, wherein, two distance sleeves are respectively arranged at the sides of the test strip. The operating method comprises the following steps: immobilizing a color-developing agent (diphenylcarbazide) into the test strip; placing the obtained test strip in a photoelectric sensor, and then dropping a Cr-containing sample solution into the test strip for full color development reaction; and utilizing the sensor to irradiate the test strip by virtue of monochromatic light, then collecting green reflected light and finally converting the green reflected light into an electrical signal. The reflectivity of the developed test strip and corresponding Cr concentration show good linear relation after color development, the detection limit is less than 5mol/L, and the test time is less than 3 minutes. Repetitive experimental data proves that the method has the advantages of good repeatability and short detection time; and by utilizing the method, on-site rapid reagent-instrument integrated detection of Cr ions can be realized.

The invention discloses a colorimetric analysis method for detecting kanamycin based on aptamer-modified magnetic beads and gold nanoparticles to simulate enzyme activity, and belongs to the technical field of analytical chemistry. In the present invention, the gold nanoparticles synthesized by tyrosine reduction of chloroauric acid simulate the peroxidase-like activity inherent in the enzyme AuNPs, and the gold nanoparticles modified by kanamycin-specific aptamers modify their complementary single-stranded cDNA The capture of nanoparticles, and the displacement of gold nanoparticles by the combination of kanamycin and aptamer, the peroxidation-like peroxidation of gold nanoparticles AuNPs contained in the supernatant after magnetic separation is related to the concentration of kanamycin The enzyme activity catalyzes the color reaction between the substrate tetramethylbenzidine (TMB) and H2O2, realizes the visual detection of kanamycin, and uses the linear relationship between the absorption value at 450 nm and the concentration of kanamycin to realize the detection of kanamycin. Colorimetric quantitative analysis of kanamycin. This method has the advantages of high sensitivity and good specificity, and is suitable for the quantitative analysis of kanamycin residues in food samples such as honey.

The invention discloses an embedded type harmful gas detection system, which comprises a cylinder, a reaction gas chamber; a gas pump, a flow sensor, an illuminating apparatus, an image acquisition instrument, an embedded type ARM9 chip, a lower computer PIC16F877, a touch screen, a porphyrin array sensor, a temperature sensor, a humidity sensor and a controllable heater, wherein the cylinder is used for storing a gas to be detected; the gas pump and the flow sensor are connected in series between the cylinder and the reaction gas chamber; the illuminating apparatus is arranged at the bottom of the reaction gas chamber; the image acquisition instrument is arranged at the top part of the reaction gas chamber; the embedded type ARM9 chip is connected with the lower computer PIC16F877 through an RS232 serial port; the touch screen is connected to the embedded type ARM9 chip; and the porphyrin array sensor, the temperature sensor, the humidity sensor and the controllable heater are arranged in the reaction gas chamber. By using a principle that different color reactions appear when the porphyrin array sensor contacts the gas, and extracting, processing, performing match identification on spectral information, the system finally obtains information on the gas to be detected, displays the information and gives an early warning in time, so the system is suitable for detecting toxic, harmful and volatile organic gasses in the fields of industrial operations, chemical industry, foods, environmental protection, interior decoration, safety and the like.

The invention discloses a method for treating the wastewater in textile printing and dyeing industry. The treatment method comprises the following steps of: A. implementing pretreatment to high-chroma dye stock solution, injecting the high-chroma dye stock solution into a color reaction tank, then adding flocculant FeCl3 and adding 200-400mg/L high-chroma dye stock solution with the residence time of 5-7h; B. homogenization treatment: adding the product in step A, other waste water and waste liquid, and externally-introduced organic wastewater into a regulation pool with the residence time of 7-9h; C. biochemical treatment: leading the effluent of the regulation pool to enter a hydrolytic acidification pool, an aerobic tank and a sedimentation tank in sequence, with the residence time of 45-50h for the wastewater at the stage; and D. post-flocculation treatment: adding flocculant FeSO4 into the effluent in the step C and then implementing post-flocculation treatment. After the treatment of the method, the effluent quality conforms to the I-class standard in Table 3 of GB4287-92 Discharge Standards for the Textile Dyeing and Finishing Industrial Water Pollutant.

The invention relates to a test paper strip of using color reaction to detect the concentration of uric acid in urine. It includes test paper base material, its feature is that: enzyme color developing reagent adheres to test paper base material. The beneficial effect of this invention is that, the detector can detect the concentration of uric acid in urine in own home, so as to preliminary reach the range of uric acid content in the urine, thus it is certain guiding significance for the blood uric acid content.

The invention relates to a method for rapidly identifying inferior edible oil. According to the method, whether an edible oil sample contains cholesterol and amino acid impurities is determined to visually, accurately and rapidly identify inferior edible oil. The method contains the following steps of: firstly adding potassium hydroxide and ethanol into the edible oil sample for saponification, adding petroleum ether and sodium chloride solution for centrifugal extraction, respectively adding a jarosite reagent and a ninhydrin reagent into an upper layered extract and a lower layered extract for color development reaction, determining whether the edible oil sample contains corresponding exogenous substances through observation of color change and simultaneously carrying out quality identification. The method provided by the invention is applicable to the detection of inferior edible oil in food hygiene and safety supervision and inspection.

The invention provides a kit for detecting the total polyphenol content in beverage, a preparation method of the kit and a method for detecting the total polyphenol content in beverage using the kit, and belongs to the field of food nutrition detection. The kit includes a coloring agent, a color stabilizer and a standard substance. According to the invention, an optimized Folio-Ciocalteu method is adopted; a beverage sample develops color after being subjected to a water bath reaction with a combined reagent at 45 DEG C for 0.5 h, and the color reaction time is reduced; the application of the kit to ultraviolet spectrophotometers and semi-automatic biochemical analyzers is simple, easy to operate and high in sensitivity, and a standard curve is not required, so that the detection time is greatly reduced, especially the application to the semi-automatic biochemical analyzers, the detection result can be read visually, and the detection difficulty is reduced; a plurality of beverage samples can be subjected to coloration and detection at the same time, so that the detection is efficient, accurate and fast, and batch detection is facilitated; therefore, the kit and detection method, provided by the invention, have great application prospects in detecting the total polyphenol content in liquid or solid beverage.

The invention relates to the technical field of chlorine dioxide disinfection sterilization, in particular to a method for evaluating a chlorine dioxide release rate. The method provided by the invention comprises the following steps: firstly forming a colorimetric card showing one-to-one correspondence between chlorine dioxide release rates and color spots, then detecting color spots of to-be-evaluated samples in the same test environment and comparing with the colorimetric card, thus the chlorine dioxide release rates of the to-be-evaluated samples can be directly read according to the colorimetric card. The method provided by the invention has the advantages that the to-be-evaluated samples only need to be placed in a test container for preset time after a color spot-release rate colorimetric card is formed, then chromogenic reaction is performed by using wet potassium iodide test paper, and reading is performed by comparing the colorimetric card, so that operation is simple and easy; and especially when the chlorine dioxide release rate of a to-be-evaluated sample needs to be tracked for a long time, only simple potassium iodide test paper chromogenic reaction and comparison with a pre-prepared color spot-release rate colorimetric card need to be performed, so that evaluation work is greatly simplified and evaluation efficiency is improved. The method provided by the invention is easy to operate, low in cost and quick in result and is applicable to popularization and application.

The invention relates to a method for quantitatively detecting alpha-synuclein auto-antibodies in human sera, and prepares recombinant human alpha-synuclein as antigens and rabbit anti-human alpha-synuclein polyclonal antibodies as antibodies into a kit, wherein, the quantities of alpha-synuclein auto-antibodies in the sera of patients with Parkinson's disease (PD) and in the sera of normal healthy persons are detected by utilizing the antigen-antibody reaction principle and are compared; and the presence and quantity of alpha-synuclein auto-antibodies in human sera are judged by the kit on the basis of color reaction.

The invention discloses a rapid detection method of synthetic pyrethroid pesticide residual, which comprises the following steps: (1) extraction the detected material: putting the synthetic pyrethroid pesticide residual in the reactor; (2) decomposition, gas production reaction: decomposing the detected material into compound within cyanide; adding the solid particle of sodium borohydride and adsorption host to mix with the detected material; adding sulphuric acid solution in the reactor to react large amount of gas; (3) colour reaction: guiding the decomposed gas in the absorber; reacting with the specific developer to make solution display blue; (4) qualitative analysis: generating qualitative or semiquanlitative analysis of synthetic pyrethroid pesticide in comparison with the colorimetric card.

This invention relates to a quick test reagent box and its application of nitrite composed of a reaction post, colorimetric cylinder, 1-naphthol, sulfanilic acid hydrochloric acid solution, potassium ferrocyanide, zine acetate, borax saturated solution and a comparison card. The test method includes: preparing a sample process fluid to be processed in chromogenic reaction to compare with the comparison card and determine the content of nitrite in the sample to judge if it has exceeded the target by transformation, which can semiquantitatively test nitrite in foodstuff and the lowest detection limit is 0.1mg/kg.

The invention discloses a method to detect Tadalafil and its derivative. It contains steps: (1) Color reaction. Make color reaction by adding concentrated sulfuric acid. (2) Result estimation. If color reaction presents blue violet, there are Tadalafil and its derivative in test samples. The method has merits of quick, simple, high sensitivity and strong property etc.

The invention discloses a method to test Sildenafil citrate and its ramifications. It contains steps: (1) Color reaction. Make color reaction of test sample with bismuth potassium iodide, silico-tungstic acid and Hager's reagent to observe result. (2) Result estimation. If above three color reactions appear deposit, there are Sildenafil citrate and its ramifications in test samples. If just one, two or none color reaction appear deposit, there is no Sildenafil citrate and its ramifications in test samples. The method has merits of quick, simple, strong property, high accuracy, extensive application and testing range.

The invention discloses a gas detection analyzer based on an intelligent terminal and a testing method; the gas detection analyzer comprises to-be-measured gas test paper or a to-be-measured gas reagent and the intelligent terminal; the to-be-measured gas test paper or the to-be-measured gas reagent is chromogenic test paper or a chromogenic reagent, and comprises chemical substances capable of performing chromogenic reaction with a to-be-measured gas, the to-be-measured gas test paper or the to-be-measured gas reagent is used for chromogenic reaction after being contacted with the to-be-measured gas, and the degree of the change is proportional to the concentration of the to-be-measured gas. By use of video acquisition and image analysis contrast functions of the intelligent terminal, the detection of the concentration of the to-be-measured gas can be realized, the operation mode is simple, and the detection precision is more accurate.