329 results about "Polyclonal antibodies" patented technology

The invention discloses a polystyrene micro-nano plastic immunochromatography time-resolved fluorescence kit and application thereof.The kit comprises a fluorescent test strip and a sample reaction bottle containing a quantum dot fluorescent microsphere labeled anti-polystyrene monoclonal antibody freeze-dried product, and the fluorescent test strip comprises a bottom plate; a water absorption pad, a detection pad and a sample pad are sequentially adhered to the adhesive surface of the bottom plate from top to bottom, adjacent pads are overlapped and connected at joints, a nitrocellulose membrane serves as a base pad of the detection pad, a transverse quality control line and a detection line are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with an anti-mouse-free polyclonal antibody, and the detection line is coated with an anti-mouse-free polyclonal antibody. And the detection line is coated with a polystyrene-hemocyanin conjugate. The immunochromatography time-resolved fluorescence kit provided by the invention can realize quantitative detection of polystyrene, and has the advantages of strong specificity, high sensitivity, simple sample pretreatment, small pollution hazard to the environment, suitability for on-site batch detection and the like.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention relates to the technical field of biological detection, and particularly discloses a test strip and a kit for detecting Alzheimer-related neurofilament protein AD7c-NTP. The detection test strip for the Alzheimer-related neurofilament protein AD7c-NTP provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; a mouse anti-AD7c-NTP monoclonal antibody I marked by latex is contained on the combination pad; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a mouse anti-AD7c-NTP monoclonal antibody II, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The detection test strip for Alzheimer-related neurofilament protein AD7c-NTP provided by the invention has the advantages of high sensitivity, strong specificity, good stability and the like, can realize auxiliary diagnosis of Alzheimer's disease, and has a wide application prospect.

The invention belongs to the technical field of biological detection, and discloses a kit and a method for quantitatively detecting residual quantity of host cell protein of pichia pastoris in order to solve the problems of limit of quantitation and low detection sensitivity of an existing detection method. According to the present invention, the empty plasmid without the recombinant human serum albumin gene is introduced into the pichia pastoris, and purification is performed by combining the Protein A chromatographic column and the host cell protein coupling affinity column, such that the prepared polyclonal antibody can be effectively used for the quantitative detection of the residual amount of the pichia pastoris host cell protein in the biological product; the kit formed by the polyclonal antibody has good accuracy and repeatability on the quantitative detection of the host cell protein residues in the pichia pastoris source biological products, the quantification limit is 0.2 ng / mL, and the kit provided by the invention can meet the quantitative detection of the host cell protein residues in all the pichia pastoris source biological products.

The invention discloses a rabbit polyclonal antibody for specific recognition of ANP32a K236 milk acylation modification and application of the rabbit polyclonal antibody. The rabbit polyclonal antibody is prepared by taking modified polypeptide as an immunogen and immunizing animals. The amino acid sequence of the adopted modified polypeptide is as shown in SEQ ID NO.1, and the 7th site in the amino acid sequence is modified by a lactic acid group and is CGSQKR-(lactyl) K-REPDD. The rabbit polyclonal antibody has high specificity and high sensitivity, can be used for detecting the milk acylation modification of the K236 site of the ANP32a protein, and fills the blank of detection tools in the field.

The invention provides a serum free light chain detection reagent and method, and the reagent comprises a first reagent which comprises a reaction buffer solution and an anti-human IgG-Fab polyclonal antibody; the second reagent comprises an anti-human FLC polyclonal antibody marked by nano microspheres; wherein the anti-human IgG-Fab polyclonal antibody is used for blocking a cross reaction between a binding light chain in immune globulin in a sample and the anti-human FLC polyclonal antibody. The anti-human IgG-Fab polyclonal antibody is added into the reaction buffer solution, so that the problem of cross reaction between the anti-FLC polyclonal antibody and a light chain combined on immune globulin can be effectively solved, and meanwhile, the long-term stability of the reagent is ensured.

The invention discloses an influenza C virus HEF protein polyclonal antibody preparation method, which comprises: carrying out signal peptide prediction, antigen epitope screening and hydrophobicity analysis through bioinformatics, constructing a recombinant prokaryotic expression vector pET32a-HEF by using a seamless cloning technology, and efficiently expressing the HEF protein in Escherichia coli to obtain the influenza C virus HEF protein polyclonal antibody. The inclusion body protein yield is improved by optimizing conditions; and purifying by nickel column affinity chromatography to obtain high-purity recombinant protein, emulsifying the high-purity recombinant protein and a Freund's adjuvant, immunizing a Japanese white rabbit, immunizing for three times, and collecting high-titer serum. The method breaks through detection limitation caused by antigen tag concealment in a eukaryotic expression system, the obtained polyclonal antibody is high in specificity, the eukaryotic expression HEF protein can be accurately recognized, and a key tool is provided for ICV virus-like particle identification, vaccine research and development and diagnosis technologies. Compared with a traditional method, the scheme is simple and convenient to operate, low in cost and high in antibody titer, and has remarkable application value.

The invention relates to the technical field of biological detection, and particularly discloses a bovine brucella antibody immunochromatography detection test strip as well as a preparation method and application thereof. The bovine brucella antibody immunochromatography detection test strip provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; the combination pad contains a mouse anti-bovine IgG monoclonal antibody marked by quantum dot microspheres; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a brucella LPS antigen, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The bovine brucella antibody immunochromatography detection test strip provided by the invention has the advantages of no subjective influence of a detector, simplicity and convenience in operation, high detection sensitivity and specificity and the like.

The invention belongs to the field of biological medicines, and particularly relates to an antigen-antibody compound for enhancing immune response of hepatitis B patients as well as a preparation method and application of the antigen-antibody compound. The antigen-antibody compound preparation prepared from the HBsAg and the anti-HBsAg monoclonal antibody is superior to an antigen-antibody compound preparation prepared from a polyclonal antibody in the aspect of continuously inducing the immune response of a chronic hepatitis B patient, and an antigen-antibody compound for inducing the immune response of the hepatitis B patient is developed for the first time; in the compound, the hepatitis B surface antigen is combined with the anti-HBsAg monoclonal antibody, and the compound formed by the hepatitis B surface antigen and the anti-HBsAg monoclonal antibody in a certain mass ratio can induce immune response of chronic hepatitis B patients and can also prevent and / or treat chronic hepatitis B.

The invention discloses a polyclonal antibody of a grouper iridovirus SGIV VP101 gene and application of the polyclonal antibody. The polyclonal antibody is prepared from an SGIVVP101 gene, and the nucleotide sequence of the VP101 gene of an optimized codon is as shown in SEQ ID NO. 1. The polyclonal antibody of the SGIV VP101 gene recombinant expression protein prepared by the invention has good specificity, not only can specifically recognize a prokaryotic expression product of the SGIV VP101, but also can be used for quantitative and positioning analysis of the SGIV VP101 in vivo and in vitro. The successful preparation of the polyclonal antibody of the recombinant expression protein of the SGIV VP101 gene lays a foundation for the establishment of an SGIV immunological detection method, the quantitative and positioning analysis of the protein of the VP101 gene and the analysis of gene functions.

The invention discloses a preparation method of a myeloid cell triggered receptor 2 protein polyclonal antibody and application of the polyclonal antibody in inhibition of renal fibrosis, and belongs to the technical field of medical molecular biology. The myeloid cell triggered receptor 2 protein is expressed by macrophages, and verification in the mechanism aspect is carried out on the protein; meanwhile, the polyclonal antibody is synthesized according to the polypeptide of the protein, then the effect of the antibody on treating the renal fibrosis is verified again, and a more targeted and personalized method is provided for diagnosis, treatment and management of the renal fibrosis and related kidney diseases.

The invention discloses an application of an AP2-F2 protein polyclonal antibody as a cryptosporidium female gamete positioning tag antibody. The AP2-F2 protein of cryptosporidium parvum is subjected to truncated prokaryotic expression, protein is purified to immunize an ICR mouse, a polyclonal antibody is prepared, ELISA and WB detection of antibody titer are carried out to prove that the polyclonal antibody is effective, then indirect immunofluorescence detection of the antibody is carried out to determine that the polyclonal antibody is located on the female gametes of cryptosporidium parvum, and the polyclonal antibody can be used as a label to locate the female gametes of cryptosporidium parvum. The method plays an important role in localization research of unknown protein of cryptosporidium.

In one aspect, a sensor for detecting a drug of abuse (DOA) in a sample is disclosed. In some embodiments, the sensor includes a graphene layer, a plurality of binding agents, which bind to the DOA, coupled to said graphene layer to generate a functionalized graphene layer, and a plurality of electrical conductors electrically coupled to said functionalized graphene layer for measuring an electrical property of said functionalized graphene layer. While in some embodiments, such binding agents are monoclonal antibodies, in other embodiments they are polyclonal antibodies.

The invention relates to the technical field of biology, in particular to a chicken CKAP4 protein polyclonal antibody and application and a kit thereof. An animal is immunized with the CKAP4 recombinant protein, serum is purified from the immunized animal, the chicken CKAP4 protein polyclonal antibody is obtained, and the amino acid sequence of the CKAP4 recombinant protein is shown as SEQ ID No.1. Sensitivity and specificity detection tests on the chicken CKAP4 protein polyclonal antibody find that the serum titer of CKAP4 is 1: 102400, the expression of the CKAP4 protein mainly focuses on cytoplasm parts of various organs of animals, and the polyclonal antibody can specifically bind to the CKAP4 protein in animal tissues. Therefore, the polyclonal antibody has the advantages of high sensitivity, good specificity, wide application range and the like, and a foundation is laid for later detection of CKAP4 protein and research on pathological mechanisms of virus infection diseases.

The invention relates to the technical field of antibodies, in particular to antigen peptides of human PP1 alpha protein and Thr320 site phosphorylated protein of the human PP1 alpha protein, and a preparation method and application of an antibody of the antigen peptides of the human PP1 alpha protein and the Thr320 site phosphorylated protein. The invention provides an antigen peptide of human PP1 alpha protein and Thr320 site phosphorylated protein thereof, the antigen peptide has obvious advantages in the aspects of immunogenicity and the like, animals can be induced to generate high-level antibodies, and the generated antibodies have high specificity, affinity and titer. Based on the antigen peptide, the invention provides an anti-human PP1 alpha protein or a polyclonal antibody with phosphorylated Thr320 site thereof and a preparation method thereof, the polyclonal antibody has the characteristics of strong specificity, high affinity and high titer, and through immunoblotting and immunohistochemical verification, the polyclonal antibody can be used for preparing the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein. The probe shows good specificity and sensitivity in detection of phosphorylation modification of human PP1 alpha protein and Thr320 site thereof, and has a good application prospect.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The invention discloses a porcine epidemic diarrhea virus S protein monoclonal antibody and application thereof. Specifically, two monoclonal antibodies GC42G2-1 and GC33D8-1 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained firstly, the GC42G2-1 antibody has good universality and can have strong affinity with different strains, and the GC33D8-1 antibody can be used for binding the porcine epidemic diarrhea virus S protein in a broad-spectrum manner. The GC42G2-1 can be independently used, and also can be matched with a polyclonal antibody or GC33D8-1 to be used for sandwich ELISA (enzyme-linked immunosorbent assay) so as to detect and quantify PEDV (porcine epidemic diarrhea virus). Secondly, the two monoclonal antibodies are respectively used as a coating antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method disclosed by the invention has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and can be used for the development of blocking ELISA (Enzyme-Linked Immunosorbent Assay), capturing ELISA, indirect ELISA, competitive ELISA and detection of porcine epidemic diarrhea viruses by colloidal gold test strips.

The invention belongs to the technical field of immunodetection, and discloses an avian single-chain antibody for detecting a liver cancer marker GPC3, a kit and a detection method. The poultry-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a chicken-derived single-chain antibody is coated on a solid-phase carrier, a rabbit-derived polyclonal antibody is taken as a detection carrier, the antibodies are combined with a GPC3 antigen to form a sandwich structure, and the double-antibody sandwich immunoassay method of the GPC3 is established by using the method, and has the advantages of high specificity, good sensitivity, high sensitivity and high sensitivity. The invention provides a rapid, accurate and reliable detection method for detecting GPC3 in serum.

The invention relates to a kit for jointly detecting markers A beta 1-40 and A beta 1-42 as well as a preparation method and application of the kit, and relates to the technical field of immunodetection. The kit comprises a first reagent and a second reagent, the first reagent comprises a magnetic bead working solution and a magnetic microsphere coated with an A beta polyclonal antibody; and the second reagent comprises a labeled antibody diluent and an acridinium ester labeled anti-A beta antibody. According to the method, an anti-A beta 1-40 antibody and an anti-A beta 1-42 antibody with high specificity are adopted, and the ratio of A beta 1-40 to A beta 1-42 is calculated; a magnetic bead working solution and a labeled antibody diluent are adopted, so that the sensitivity and the stabilizer of the kit are further improved; the detection time is 8 minutes, the result can be obtained, the repeatability is within 4%, the lowest detection limit of A beta 1-40 is 1.08 pg / ml, and the lowest detection limit of A beta 1-42 is 1.08 pg / ml.

The invention discloses a duck BRS3 polypeptide and a preparation method and application of a polyclonal antibody of the duck BRS3 polypeptide, and belongs to the field of biochemistry and molecular immunology. The amino acid sequence of the duck BRS3 polypeptide is as shown in SEQ ID NO. 3. A segment of polypeptide sequence with the length of 20 aa is screened out in an intracellular region of a duck BRS3 protein sequence, after artificial solid-phase synthesis, the polypeptide sequence is used as an immunizing antigen to immunize New Zealand white rabbits, and the duck BRS3 protein polyclonal antibody with the antiserum titer being 1: 25600 or above is obtained. The rabbit anti-duck BRS3 polyclonal antibody prepared by the invention can be specifically combined with the BRS3 protein in duck tissues, so that the blank in the field of detection and research of the duck BRS3 protein is filled, and a foundation is laid for research of functions of the BRS3 in duck bodies.

The invention provides an anti-grouper PSMB8 protein polyclonal antibody as well as a preparation method and application thereof. The anti-grouper PSMB8 protein polyclonal antibody is obtained by taking recombinant protein EcPSMB8 as an antigen immune animal, and the amino acid sequence of the recombinant protein EcPSMB8 is as shown in SEQ ID NO: 1. The polyclonal antibody for resisting the grouper PSMB8 protein, provided by the invention, has high specificity on the grouper PSMB8 protein, the problem that a high-specificity antibody for the grouper PSMB8 protein is lacked in the prior art is solved, and the grouper PSMB8 protein can be better and more effectively researched.

The invention provides a preparation method and application of a polyclonal antibody for recognizing IL-33 protein K6 site lactic acid modification, and belongs to the technical field of biological medicine, the invention discloses that lactic acid modification of IL-33 protein 6th lysine (Lys6) plays a key role in occurrence and development of liver cancer, and experiments prove that the site lactic acid promotes liver cancer cell proliferation and tumor growth; based on the discovery, the invention innovatively proposes that Lys6 lactic acid is used as a new target for tumor diagnosis and treatment, and successfully develops an antigen peptide and an antibody tool for specifically recognizing the modification site; the preclinical invention shows that the progress of the liver cancer can be effectively inhibited by blocking the Lys6 lactylation, the constructed detection system shows high sensitivity and specificity in liver cancer diagnosis, and the developed targeted antibody drug also has remarkable anti-tumor activity.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

A polyclonal antibody composition specifically binds the pTonB epitope (SEQ ID NO: 11) from Acinetobacter baumannii and is elicited by immunization with a multi-epitope antigen comprising thioredoxin leader, rigid linker, and kernels including SEQ ID NOs: 6, 7, 11, 12, and 8. These antibodies enhance opsonophagocytic killing of A. baumannii Ci79 via classical complement activation and Fey receptor-mediated phagocytosis by bone marrow-derived macrophages. Absorption of pTonB-specific antibodies reduces killing by >70%, confirming epitope dominance. Passive transfer of the composition protects >60% of mice in a lethal intranasal challenge model. Pharmaceutical compositions, treatment methods (alone or with antibiotics like colistin), prophylactic uses, diagnostic kits, and polyclonal compositions are disclosed for combating multidrug-resistant A. baumannii infections.

This invention belongs to the field of biology, specifically relating to a method for preparing polyclonal antibodies based on a specific fragment of the capsid protein of Chrysanthemum B virus. This invention discloses the antigenic determinant CVB-CP-B and its acquisition method, as well as the antigen CVB-CP-B obtained from the antigenic determinant, and Ab-CVB-CP-B, which facilitates the preparation of antibodies from antigen CVB-CP-B. This invention also discloses the application of the above-mentioned antibodies in the detection of Chrysanthemum B virus.

The present disclosure discloses a detection kit and a preparation method thereof and a detection method for novel coronavirus, and relates to the technical field of biomedicine, including an antigen test strip. The antigen test strip includes a substrate, bibulous paper, immune nitrocellulose membrane, and immune microsphere pad. The immune microsphere pad, the immune nitrocellulose membrane and the bibulous paper are pasted on the substrate. The immune microsphere pad is coated with latex microsphere-labeled novel coronavirus SARS-CoV-2 monoclonal antibody 1. The immune nitrocellulose membrane is provided with a test line coated with novel coronavirus SARS-CoV-2 monoclonal antibody 2 and a quality control line coated with goat anti-mouse IgG polyclonal antibody. The present disclosure uses latex particles as labeled tracer, and uses antigen-antibody reaction and lateral chromatography to detect and analyze targets, having advantages of convenience and swift.

The invention relates to a mycobacterium tuberculosis EspB polyclonal antibody as well as a preparation method and application thereof. The purified EspB antibody has good detection specificity, only reacts with mycobacteria, and does not react with other common pneumonia pathogenic bacteria. The EspB polyclonal antibody specifically recognizes mycobacteria EspB, comprises mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) EspB which grows slowly and rapidly, and can be used for detecting tuberculosis and NTM diseases.

The invention discloses a human intragastric factor protein polyclonal antibody and a preparation method thereof. The preparation method comprises the steps of artificial antigen design, animal immune optimization and antibody purification and verification. Accurate epitope design is achieved, structural interference of natural GIF is avoided through artificial polypeptide, and the cross reaction rate is lt; 5%; an efficient immune strategy is adopted, so that the immune period is shortened to 8-10 weeks, and the titer is increased by 2-3 times; low-cost production is realized, natural protein is replaced by the synthesized polypeptide, and the cost is reduced by 60%; the specificity is high, the cross reaction is reduced due to the design of the artificial antigen, and verification data supports specific recognition of natural GIF; large-scale production, polypeptide synthesis and a double-stage purification process are adopted, so that the consistency between batches is high (CV is less than or equal to 8%); the application range is wide, and diagnosis, pathological research and treatment monitoring scenes are covered.

The application discloses a sequence of a separated new bunyavirus M segment and a Gn protein coded by the sequence, a nucleotide sequence of the M segment and an amino acid sequence coded by the nucleotide sequence, and expression, purification and activity analysis of a fusion protein formed by fusing the Gn coded by the M segment with Fc to form a Gn-Fc protein. In addition, the application also relates to cross reaction of the fusion protein with polyclonal antibodies in different patient sera.