232 results about "Polyclonal antibodies" patented technology

The invention relates to the technical field of biological detection, and particularly discloses a test strip and a kit for detecting Alzheimer-related neurofilament protein AD7c-NTP. The detection test strip for the Alzheimer-related neurofilament protein AD7c-NTP provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; a mouse anti-AD7c-NTP monoclonal antibody I marked by latex is contained on the combination pad; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a mouse anti-AD7c-NTP monoclonal antibody II, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The detection test strip for Alzheimer-related neurofilament protein AD7c-NTP provided by the invention has the advantages of high sensitivity, strong specificity, good stability and the like, can realize auxiliary diagnosis of Alzheimer's disease, and has a wide application prospect.

The invention belongs to the technical field of biological detection, and discloses a kit and a method for quantitatively detecting residual quantity of host cell protein of pichia pastoris in order to solve the problems of limit of quantitation and low detection sensitivity of an existing detection method. According to the present invention, the empty plasmid without the recombinant human serum albumin gene is introduced into the pichia pastoris, and purification is performed by combining the Protein A chromatographic column and the host cell protein coupling affinity column, such that the prepared polyclonal antibody can be effectively used for the quantitative detection of the residual amount of the pichia pastoris host cell protein in the biological product; the kit formed by the polyclonal antibody has good accuracy and repeatability on the quantitative detection of the host cell protein residues in the pichia pastoris source biological products, the quantification limit is 0.2 ng / mL, and the kit provided by the invention can meet the quantitative detection of the host cell protein residues in all the pichia pastoris source biological products.

The invention relates to the technical field of antibodies, in particular to antigen peptides of human PP1 alpha protein and Thr320 site phosphorylated protein of the human PP1 alpha protein, and a preparation method and application of an antibody of the antigen peptides of the human PP1 alpha protein and the Thr320 site phosphorylated protein. The invention provides an antigen peptide of human PP1 alpha protein and Thr320 site phosphorylated protein thereof, the antigen peptide has obvious advantages in the aspects of immunogenicity and the like, animals can be induced to generate high-level antibodies, and the generated antibodies have high specificity, affinity and titer. Based on the antigen peptide, the invention provides an anti-human PP1 alpha protein or a polyclonal antibody with phosphorylated Thr320 site thereof and a preparation method thereof, the polyclonal antibody has the characteristics of strong specificity, high affinity and high titer, and through immunoblotting and immunohistochemical verification, the polyclonal antibody can be used for preparing the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein. The probe shows good specificity and sensitivity in detection of phosphorylation modification of human PP1 alpha protein and Thr320 site thereof, and has a good application prospect.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The invention belongs to the technical field of immunodetection, and discloses an avian single-chain antibody for detecting a liver cancer marker GPC3, a kit and a detection method. The poultry-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a chicken-derived single-chain antibody is coated on a solid-phase carrier, a rabbit-derived polyclonal antibody is taken as a detection carrier, the antibodies are combined with a GPC3 antigen to form a sandwich structure, and the double-antibody sandwich immunoassay method of the GPC3 is established by using the method, and has the advantages of high specificity, good sensitivity, high sensitivity and high sensitivity. The invention provides a rapid, accurate and reliable detection method for detecting GPC3 in serum.

The invention discloses a duck BRS3 polypeptide and a preparation method and application of a polyclonal antibody of the duck BRS3 polypeptide, and belongs to the field of biochemistry and molecular immunology. The amino acid sequence of the duck BRS3 polypeptide is as shown in SEQ ID NO. 3. A segment of polypeptide sequence with the length of 20 aa is screened out in an intracellular region of a duck BRS3 protein sequence, after artificial solid-phase synthesis, the polypeptide sequence is used as an immunizing antigen to immunize New Zealand white rabbits, and the duck BRS3 protein polyclonal antibody with the antiserum titer being 1: 25600 or above is obtained. The rabbit anti-duck BRS3 polyclonal antibody prepared by the invention can be specifically combined with the BRS3 protein in duck tissues, so that the blank in the field of detection and research of the duck BRS3 protein is filled, and a foundation is laid for research of functions of the BRS3 in duck bodies.

The invention provides a preparation method and application of a polyclonal antibody for recognizing IL-33 protein K6 site lactic acid modification, and belongs to the technical field of biological medicine, the invention discloses that lactic acid modification of IL-33 protein 6th lysine (Lys6) plays a key role in occurrence and development of liver cancer, and experiments prove that the site lactic acid promotes liver cancer cell proliferation and tumor growth; based on the discovery, the invention innovatively proposes that Lys6 lactic acid is used as a new target for tumor diagnosis and treatment, and successfully develops an antigen peptide and an antibody tool for specifically recognizing the modification site; the preclinical invention shows that the progress of the liver cancer can be effectively inhibited by blocking the Lys6 lactylation, the constructed detection system shows high sensitivity and specificity in liver cancer diagnosis, and the developed targeted antibody drug also has remarkable anti-tumor activity.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

A polyclonal antibody composition specifically binds the pTonB epitope (SEQ ID NO: 11) from Acinetobacter baumannii and is elicited by immunization with a multi-epitope antigen comprising thioredoxin leader, rigid linker, and kernels including SEQ ID NOs: 6, 7, 11, 12, and 8. These antibodies enhance opsonophagocytic killing of A. baumannii Ci79 via classical complement activation and Fey receptor-mediated phagocytosis by bone marrow-derived macrophages. Absorption of pTonB-specific antibodies reduces killing by >70%, confirming epitope dominance. Passive transfer of the composition protects >60% of mice in a lethal intranasal challenge model. Pharmaceutical compositions, treatment methods (alone or with antibiotics like colistin), prophylactic uses, diagnostic kits, and polyclonal compositions are disclosed for combating multidrug-resistant A. baumannii infections.

This invention belongs to the field of biology, specifically relating to a method for preparing polyclonal antibodies based on a specific fragment of the capsid protein of Chrysanthemum B virus. This invention discloses the antigenic determinant CVB-CP-B and its acquisition method, as well as the antigen CVB-CP-B obtained from the antigenic determinant, and Ab-CVB-CP-B, which facilitates the preparation of antibodies from antigen CVB-CP-B. This invention also discloses the application of the above-mentioned antibodies in the detection of Chrysanthemum B virus.

The invention relates to a mycobacterium tuberculosis EspB polyclonal antibody as well as a preparation method and application thereof. The purified EspB antibody has good detection specificity, only reacts with mycobacteria, and does not react with other common pneumonia pathogenic bacteria. The EspB polyclonal antibody specifically recognizes mycobacteria EspB, comprises mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) EspB which grows slowly and rapidly, and can be used for detecting tuberculosis and NTM diseases.

The application discloses a sequence of a separated new bunyavirus M segment and a Gn protein coded by the sequence, a nucleotide sequence of the M segment and an amino acid sequence coded by the nucleotide sequence, and expression, purification and activity analysis of a fusion protein formed by fusing the Gn coded by the M segment with Fc to form a Gn-Fc protein. In addition, the application also relates to cross reaction of the fusion protein with polyclonal antibodies in different patient sera.

The invention belongs to the technical field of Ultrap1 enzyme quantitative detection, and particularly relates to an Ultrap1 enzyme double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) kit and application. Comprising an elisa plate coated with an anti-Ultrap1 enzyme mouse monoclonal antibody, an anti-Ultrap1 enzyme rabbit polyclonal antibody, an HRP-labeled goat anti-rabbit antibody, a diluent, a cleaning solution, a confining solution, a developing solution and a stop solution. According to the present invention, the complex of the Ulp1 enzyme and the SUMO tag protein is adopted as the immunogen to simultaneously immunize mice and New Zealand rabbits so as to prepare different species of the good affinity Ulp1 enzyme antibodies, and then the optimal reaction condition is further screened to develop the stable double-antibody sandwich ELISA detection kit with characteristics of high specificity and strong sensitivity, the defects in the prior art can be effectively overcome.

The invention relates to the technical field of biological medicines, and discloses a construction method and expression application of an Hcp gene deleted strain of a rahnella aqua KCL-5 strain for fish. The Hcp 0186 gene deletion strain is constructed by using a homologous recombination method, the comparison between the deletion strain and a wild strain is carried out, and the result shows that the deletion of the Hcp 0186 gene has an influence on the growth speed, the pathogenicity of the Hcp 0186 gene to the zebra fish is obviously reduced, the survival rate of the infected zebra fish is increased from 0% to 52%, and the Hcp 0186 gene deletion strain can be used as a fish attenuated live vaccine. A recombinant prokaryotic expression vector pET32a-Hcp and expression and purification of fusion protein of the recombinant prokaryotic expression vector pET32a-Hcp are further constructed, and Hcp 0186 rabbit polyclonal antibody serum is prepared as a primary antibody and can be applied to localization expression analysis of fish tissues infected by the bacterium and detection of WB and IHC. Results prove that the Hcp 0186 gene deletion of the strain provides an attenuated strain vaccine and clinical diagnosis and detection application of infected fish of the attenuated strain vaccine, and a scientific basis can be provided for revealing a molecular pathogenic mechanism of the strain and development and application of a mutant strain of the strain.

The application discloses a monoclonal antibody of a rice field eel Adam8a and a preparation method and application thereof, relates to the technical field of biological antibodies, and fills the research blank of the rice field eel Adam8a. The rice field eel Adam8a monoclonal antibody is high in specificity and titer, simple in preparation process, and controllable in cost, can be widely used for recognizing Adam8a homologous proteins in different fish ovary, and is suitable for being prepared into a fish ovary Adam8a protein detection kit, and is compared with the expression of the protein in different fish, so that the rice field eel Adam8a monoclonal antibody becomes a very convenient detection tool in the biological research of fish ovary development.

The invention discloses a crassostrea hongkongensis NKA-alpha protein polyclonal antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: constructing a recombinant expression vector containing an NKA-alpha target gene segment; transforming the recombinant expression vector into competent cells to construct a recombinant expression strain, inducing the recombinant expression strain to express fusion protein, and collecting and purifying to obtain NKA-alpha recombinant protein; carrying out animal immune treatment by taking the NKA-alpha recombinant protein as an antigen, collecting and separating antiserum, and purifying to obtain the Crassostrea hongkongensis NKA-alpha protein polyclonal antibody. The prepared Crassostrea hongkongensis NKA-alpha polyclonal antibody is high in affinity, can specifically recognize NKA-alpha protein in Crassostrea hongkongensis, and can be widely applied to Crassostrea hongkongensis biological immunodetection.

The invention discloses a preparation method and application of carassius auratus rahn aquatic OmpA gene cloning and prokaryotic expression as well as a polyclonal antibody. The preparation method comprises the following steps: culture and DNA amplification of rahn aquatic, design of an OmpA primer, gene cloning and prokaryotic expression, back multi-point subcutaneous injection of an immune rabbit, heart blood sampling and preparation of the polyclonal antibody. The invention further provides OmpA protein prokaryotic expression and protein purification, preparation of OmpA polyclonal antiserum and application of fish bodies. According to the invention, a rahnella aquatilis OmpA gene is cloned, a specific Hind III and EcoR1 double-enzyme digestion primer sequence OmpA-1F / OmpA-1R is designed, a prokaryotic expression vector pET32a-OmpA is constructed, high-immunogenicity OmpA protein is obtained through purification, OmpA specific multi-antibody serum is prepared, and the OmpA specific multi-antibody serum can be used for preparing the multi-antibody serum. The OmpA polyclonal antibody can be used for detecting and positioning the expression application of rahnella aquatica OmpA protein in crucian carp bodies through bidirectional agar diffusion, an immunoblotting method and an immunohistochemical method, and provides a reference basis for research and development and application of rahnella aquatica subunit vaccines and a molecular diagnosis technology as well as immune prevention and control of infectious diseases of the rahnella aquatica subunit vaccines and the molecular diagnosis technology.

The present invention is directed to the field of in vitro diagnostics and relates to reagents containing non-blood-based antibodies for determining an analyte in an immunoprecipitation assay and having a sensitivity comparable to conventional reagents containing polyclonal antibody sera.

The invention discloses a polyclonal antibody of panda Rbp7 protein as well as a preparation method and application thereof, and belongs to the technical field of antibodies. The first to nineteenth amino acid sequences of the panda Rbp7 protein are used as antigen polypeptides, and are specifically shown as SEQ ID No.1. After chemical synthesis or exogenous expression purification, a Balb / C mouse is immunized, and the polyclonal antibody is obtained through repeated immunization, serum titer detection and affinity chromatography purification. The titer of the antibody is not lower than 1 / 128000, the panda Rbp7 recombinant protein can be efficiently and specifically detected through WB and ELISA, the blank of a protein detection tool is filled, and reliable technical support is provided for research on the functional mechanism of the panda Rbp7 protein.

The present application relates to the antibody of phorbolylated isocitrate dehydrogenase 1 and its preparation method and purpose, and specifically provides a polyclonal antibody, the polyclonal antibody is obtained by immunizing animals with the antigen epitope peptide of IDH1 phorbolylated, the specific site of the antigen epitope peptide is phorbolylated, and high-titer antibodies can be prepared, the phorbolylated modification of IDH1 in clinical specimens can be recognized with high specificity, and the polyclonal antibody can be used for the research of cell / tissue specimen immunoblotting and immunohistochemical method and the diagnosis of IDH1 related diseases.

The invention discloses a polyclonal antibody targeting a human CPNE7 variable splicing isomer and application of the polyclonal antibody, and belongs to the technical field of biological medicine. The antigen epitope 'KYKQKRRSYKN' (SEQ ID NO.1) capable of being targeted by the polyclonal antibody provided by the invention is positioned in a common conserved region of all main isomers of CPNE7, and a variable splicing region is not involved. The titer of the obtained antibody serum is as high as 1: 32,000, which indicates that the antibody has extremely high immunoreactivity and sensitivity and can be used for detecting trace proteins. The antibody disclosed by the invention can be used for effectively identifying GST-CPNE7 fusion protein expressed by a prokaryotic system and CPNE7-FL and CPNE7-S protein overexpressed in an eukaryotic system (A549 cells), and the applicability of the antibody in various experimental systems is proved. Therefore, the blank in the prior art can be filled, and a core tool is provided for research of the CPNE7 in the fields of tumor biology, diagnosis and treatment.

The application discloses a monoclonal antibody specifically combined with PGLYRP1 and a preparation method and application thereof. The monoclonal antibody capable of accurately recognizing PGLYRP1 is constructed, and the monoclonal antibody can stably recognize the conservative domain of PGLYRP1. The monoclonal antibody can significantly reduce cross-reaction and improve the accuracy of experimental results, and is particularly suitable for signal detection in complex tissues. In addition, the monoclonal antibody is derived from a stable cell clone, can be cultured and amplified for a long time, and ensures the quality stability of the product, so that the problems of large batch-to-batch difference and non-reproducibility of polyclonal antibodies are overcome.

The invention discloses a halogen modified hapten for enhancing antibody titer and affinity as well as a preparation method and application of the halogen modified hapten, and belongs to the technical field of biochemical engineering. According to the method, an amantadine structure is innovatively and chemically modified, various halogens such as fluorine, chlorine and bromine are introduced, the AMA halogen modified hapten shown in the formula (I) is formed, and the traditional'most similar 'design principle is broken through. Compared with an unmodified hapten, the hapten modified by halogen in the invention has the advantages that the titer of the polyclonal antibody on amantadine can be obviously improved by 3.4-14 times; wherein the haptens A5 and A6 can obviously improve the titer of the monoclonal antibody to amantadine by 3-18 times, and the affinity is improved by 10.8-16.1 times. The invention provides a new thought and a new method for the rational design of the hapten of the small molecule compound and the preparation of the high-affinity antibody, and the related halogen modification type and quantity strategy can also provide valuable reference for the design of the hapten of other small molecule compounds.

The application discloses a fluorescence detection method and a kit for rapidly detecting mycotoxin roquefortine in a dual-purpose medicinal material, and a rapid fluorescence detection kit for mycotoxin roquefortine, which comprises the following components: A) a roquefortine standard solution; B) a roquefortine polyclonal antibody modified magnetic nano material; and C) a roquefortine polyclonal antibody modified up-conversion nano material. The detection method using the kit uses roquefortine polyclonal antibody modified magnetic nanoparticles to capture roquefortine and uses roquefortine polyclonal antibody modified up-conversion nano materials as a fluorescence probe to detect roquefortine, and has the advantages of simple method, high sensitivity and easy operation.

The invention relates to the technical field of genetic engineering, in particular to a small yellow croaker IRE1 alpha protein polyclonal antibody as well as a preparation method and application thereof. The invention provides a preparation method of a small yellow croaker IRE1alpha protein polyclonal antibody. The preparation method comprises the following steps: cloning an open reading frame of a small yellow croaker IRE1alpha gene, and constructing a small yellow croaker IRE1alpha gene recombinant expression vector pET-30a (+)-IRE1alpha. Performing inducible expression and purification on the recombinant protein of the Larimichthys polyactis IRE1 alpha, and performing specific analysis on Western Blot; and preparing the polyclonal antibody of the small yellow croaker IRE1 alpha. According to the invention, a recombinant expression vector of the small yellow croaker IRE1alpha gene is constructed by adopting molecular biology and genetic engineering methods, purified recombinant protein is obtained through induced expression and affinity chromatography, the prepared polyclonal antibody can be used for expression detection of the small yellow croaker IRE1alpha protein, and an important material foundation is laid for research on functions and action mechanisms of the small yellow croaker IRE1alpha protein.

The application belongs to the field of marine biotechnology, and specifically discloses a preparation method of a long oyster insulin-like peptide receptor (ILPR) polyclonal antibody and application thereof. The method is characterized by the following steps: specific antigen epitopes of the long oyster ILPR protein are screened through bioinformatics analysis, a pET32a-ILPR recombinant plasmid is constructed and induced to express in Escherichia coli; the purified recombinant protein is used as an immunogen to immunize New Zealand white rabbits, and rabbit antisera are collected after four times of booster immunization; specific polyclonal antibodies are purified by using antigen affinity chromatography technology, and can specifically recognize the ILPR protein in long oyster tissues. The antibody prepared by the application has high specificity and sensitivity, and provides an important biological tool for studying the insulin signal transduction pathway, growth regulation and energy metabolism mechanism of long oysters.

The invention discloses an indoor allergen active removal and intelligent control system and method, and relates to the technical field of indoor environment health and intelligent control. The system monitors the concentration of allergens in air and environmental parameters in real time through a plurality of sensor nodes; the source inhibition module acts on an allergen source through a conveying device by utilizing an IgY polyclonal antibody preparation from poultry yolk; the environment removing module comprises an air purifying device and an allergen removing device and is used for removing air and object surfaces; the control module analyzes the detection result based on the dynamic mathematical model and the feedback regulation logic, generates a control instruction, and coordinates the above modules to achieve a synergistic effect. According to the invention, real-time monitoring, source inhibition and active elimination of allergens can be realized, rapid response and adaptive adjustment can be realized under different environmental conditions, the concentration of indoor allergens can be obviously reduced, the air quality can be improved, the health risk of sensitive people can be reduced, and the application prospect is good.

The present invention relates to a multi-epitope antigen comprising 20 epitope masses of from 20 to 36 amino acids, from a protein selected from the group consisting of NanA, PcsB, PhtD, Ply, PncO, StkP, PspA-F1 and PspA-F2. In addition, the invention relates to an immunogenic composition comprising said antigen and a pharmaceutically acceptable vehicle and / or adjuvant. In addition, the invention also discloses a pneumococcus diagnostic kit, which comprises the antigen combined with a detectable fraction or a bioactive fragment thereof; an antibody generated from the antigen; and instructions for use. Furthermore, the invention relates to the use of said antigens for the preparation of vaccines for the prevention of diseases caused by pneumococcal bacteria and for the production of monoclonal and polyclonal antibodies. Finally, the present invention relates to an in vitro diagnostic method of pneumococci comprising contacting said antigen bound to a detectable fraction or a biologically active fragment thereof with a sample of bodily fluids selected from mucus and urine expelled from blood, lower respiratory tract.

The invention discloses a bridging antibody, an enzyme-labeled polymer as well as a preparation method and application of the bridging antibody. The bridging antibody is prepared by the following steps: performing enzyme digestion, purification, reduction, sulfydryl protection and coupling on a polyclonal antibody and an anti-DMT-biotin monoclonal antibody through pepsin, and then purifying to obtain a heterodimer product polyclonal antibody-Fab '-Linker-Fab'-anti-DMT-biotin; hRP is subjected to maleimide and then coupled with a sulfhydrylated polymer to obtain a polymer enzyme, and then the polymer enzyme is coupled with a small molecule DMT-biotin to obtain the enzyme-labeled polymer which is specifically combined with the bridging secondary antibody. The obtained bridging antibody and enzyme-labeled polymer are used for preparing a kit for three-stage amplification of immunohistochemical signals, through cooperation of all the components, low-abundance antigens in tissues can be better detected, and the false positive rate is greatly reduced.