109 results about "Polyclonal antibodies" patented technology

The invention belongs to the technical field of biological detection, and discloses a kit and a method for quantitatively detecting residual quantity of host cell protein of pichia pastoris in order to solve the problems of limit of quantitation and low detection sensitivity of an existing detection method. According to the present invention, the empty plasmid without the recombinant human serum albumin gene is introduced into the pichia pastoris, and purification is performed by combining the Protein A chromatographic column and the host cell protein coupling affinity column, such that the prepared polyclonal antibody can be effectively used for the quantitative detection of the residual amount of the pichia pastoris host cell protein in the biological product; the kit formed by the polyclonal antibody has good accuracy and repeatability on the quantitative detection of the host cell protein residues in the pichia pastoris source biological products, the quantification limit is 0.2 ng / mL, and the kit provided by the invention can meet the quantitative detection of the host cell protein residues in all the pichia pastoris source biological products.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

A polyclonal antibody composition specifically binds the pTonB epitope (SEQ ID NO: 11) from Acinetobacter baumannii and is elicited by immunization with a multi-epitope antigen comprising thioredoxin leader, rigid linker, and kernels including SEQ ID NOs: 6, 7, 11, 12, and 8. These antibodies enhance opsonophagocytic killing of A. baumannii Ci79 via classical complement activation and Fey receptor-mediated phagocytosis by bone marrow-derived macrophages. Absorption of pTonB-specific antibodies reduces killing by >70%, confirming epitope dominance. Passive transfer of the composition protects >60% of mice in a lethal intranasal challenge model. Pharmaceutical compositions, treatment methods (alone or with antibiotics like colistin), prophylactic uses, diagnostic kits, and polyclonal compositions are disclosed for combating multidrug-resistant A. baumannii infections.

The invention relates to a mycobacterium tuberculosis EspB polyclonal antibody as well as a preparation method and application thereof. The purified EspB antibody has good detection specificity, only reacts with mycobacteria, and does not react with other common pneumonia pathogenic bacteria. The EspB polyclonal antibody specifically recognizes mycobacteria EspB, comprises mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) EspB which grows slowly and rapidly, and can be used for detecting tuberculosis and NTM diseases.

The application discloses a sequence of a separated new bunyavirus M segment and a Gn protein coded by the sequence, a nucleotide sequence of the M segment and an amino acid sequence coded by the nucleotide sequence, and expression, purification and activity analysis of a fusion protein formed by fusing the Gn coded by the M segment with Fc to form a Gn-Fc protein. In addition, the application also relates to cross reaction of the fusion protein with polyclonal antibodies in different patient sera.

The invention relates to the technical field of biological medicines, and discloses a construction method and expression application of an Hcp gene deleted strain of a rahnella aqua KCL-5 strain for fish. The Hcp 0186 gene deletion strain is constructed by using a homologous recombination method, the comparison between the deletion strain and a wild strain is carried out, and the result shows that the deletion of the Hcp 0186 gene has an influence on the growth speed, the pathogenicity of the Hcp 0186 gene to the zebra fish is obviously reduced, the survival rate of the infected zebra fish is increased from 0% to 52%, and the Hcp 0186 gene deletion strain can be used as a fish attenuated live vaccine. A recombinant prokaryotic expression vector pET32a-Hcp and expression and purification of fusion protein of the recombinant prokaryotic expression vector pET32a-Hcp are further constructed, and Hcp 0186 rabbit polyclonal antibody serum is prepared as a primary antibody and can be applied to localization expression analysis of fish tissues infected by the bacterium and detection of WB and IHC. Results prove that the Hcp 0186 gene deletion of the strain provides an attenuated strain vaccine and clinical diagnosis and detection application of infected fish of the attenuated strain vaccine, and a scientific basis can be provided for revealing a molecular pathogenic mechanism of the strain and development and application of a mutant strain of the strain.

The application discloses a monoclonal antibody of a rice field eel Adam8a and a preparation method and application thereof, relates to the technical field of biological antibodies, and fills the research blank of the rice field eel Adam8a. The rice field eel Adam8a monoclonal antibody is high in specificity and titer, simple in preparation process, and controllable in cost, can be widely used for recognizing Adam8a homologous proteins in different fish ovary, and is suitable for being prepared into a fish ovary Adam8a protein detection kit, and is compared with the expression of the protein in different fish, so that the rice field eel Adam8a monoclonal antibody becomes a very convenient detection tool in the biological research of fish ovary development.

The invention discloses a crassostrea hongkongensis NKA-alpha protein polyclonal antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: constructing a recombinant expression vector containing an NKA-alpha target gene segment; transforming the recombinant expression vector into competent cells to construct a recombinant expression strain, inducing the recombinant expression strain to express fusion protein, and collecting and purifying to obtain NKA-alpha recombinant protein; carrying out animal immune treatment by taking the NKA-alpha recombinant protein as an antigen, collecting and separating antiserum, and purifying to obtain the Crassostrea hongkongensis NKA-alpha protein polyclonal antibody. The prepared Crassostrea hongkongensis NKA-alpha polyclonal antibody is high in affinity, can specifically recognize NKA-alpha protein in Crassostrea hongkongensis, and can be widely applied to Crassostrea hongkongensis biological immunodetection.

The present application relates to the antibody of phorbolylated isocitrate dehydrogenase 1 and its preparation method and purpose, and specifically provides a polyclonal antibody, the polyclonal antibody is obtained by immunizing animals with the antigen epitope peptide of IDH1 phorbolylated, the specific site of the antigen epitope peptide is phorbolylated, and high-titer antibodies can be prepared, the phorbolylated modification of IDH1 in clinical specimens can be recognized with high specificity, and the polyclonal antibody can be used for the research of cell / tissue specimen immunoblotting and immunohistochemical method and the diagnosis of IDH1 related diseases.

The invention discloses a halogen modified hapten for enhancing antibody titer and affinity as well as a preparation method and application of the halogen modified hapten, and belongs to the technical field of biochemical engineering. According to the method, an amantadine structure is innovatively and chemically modified, various halogens such as fluorine, chlorine and bromine are introduced, the AMA halogen modified hapten shown in the formula (I) is formed, and the traditional'most similar 'design principle is broken through. Compared with an unmodified hapten, the hapten modified by halogen in the invention has the advantages that the titer of the polyclonal antibody on amantadine can be obviously improved by 3.4-14 times; wherein the haptens A5 and A6 can obviously improve the titer of the monoclonal antibody to amantadine by 3-18 times, and the affinity is improved by 10.8-16.1 times. The invention provides a new thought and a new method for the rational design of the hapten of the small molecule compound and the preparation of the high-affinity antibody, and the related halogen modification type and quantity strategy can also provide valuable reference for the design of the hapten of other small molecule compounds.

The application belongs to the field of marine biotechnology, and specifically discloses a preparation method of a long oyster insulin-like peptide receptor (ILPR) polyclonal antibody and application thereof. The method is characterized by the following steps: specific antigen epitopes of the long oyster ILPR protein are screened through bioinformatics analysis, a pET32a-ILPR recombinant plasmid is constructed and induced to express in Escherichia coli; the purified recombinant protein is used as an immunogen to immunize New Zealand white rabbits, and rabbit antisera are collected after four times of booster immunization; specific polyclonal antibodies are purified by using antigen affinity chromatography technology, and can specifically recognize the ILPR protein in long oyster tissues. The antibody prepared by the application has high specificity and sensitivity, and provides an important biological tool for studying the insulin signal transduction pathway, growth regulation and energy metabolism mechanism of long oysters.

The present invention relates to a multi-epitope antigen comprising 20 epitope masses of from 20 to 36 amino acids, from a protein selected from the group consisting of NanA, PcsB, PhtD, Ply, PncO, StkP, PspA-F1 and PspA-F2. In addition, the invention relates to an immunogenic composition comprising said antigen and a pharmaceutically acceptable vehicle and / or adjuvant. In addition, the invention also discloses a pneumococcus diagnostic kit, which comprises the antigen combined with a detectable fraction or a bioactive fragment thereof; an antibody generated from the antigen; and instructions for use. Furthermore, the invention relates to the use of said antigens for the preparation of vaccines for the prevention of diseases caused by pneumococcal bacteria and for the production of monoclonal and polyclonal antibodies. Finally, the present invention relates to an in vitro diagnostic method of pneumococci comprising contacting said antigen bound to a detectable fraction or a biologically active fragment thereof with a sample of bodily fluids selected from mucus and urine expelled from blood, lower respiratory tract.

The invention discloses an OVA-D fluorescence immunochromatography detection test strip in a surimi product and application thereof, the test strip comprises a substrate, a sample adding pad, a combination pad, a reaction film and a water absorption pad, the sample adding pad, the combination pad, the reaction film and the water absorption pad are arranged on the substrate, the reaction film is arranged in the middle of the substrate, one end of the sample adding pad is in contact with the other end of the combination pad, one end of the combination pad is in contact with the other end of the reaction film, and the other end of the reaction film is in contact with the water absorption pad. One end of the reaction film contacts with the other end of the water absorption pad; the combination pad contains a rabbit anti-OVA-D polyclonal antibody marked by fluorescent quantum dots and goat IgG marked by fluorescent quantum dots; the reaction film is provided with a detection area, the detection area is sequentially provided with a detection line and a control line from the other end to one end of the reaction film, the detection line is coated with an OVA-D antigen, and the control line is coated with a rabbit anti-goat IgG antibody. The fluorescence signal is stable, the fluorescence intensity of the T line and the C line is not influenced by a complex matrix in a detected sample, the detection sensitivity is higher, and the reliability of quantitative detection of the duck egg ovalbumin is also ensured.

This invention relates to the field of genetic engineering technology, specifically disclosing a polyclonal antibody against citrus HIPP7 protein, its preparation method, and its applications. The method includes selecting amino acid 1-246 of the HIPP7 protein sequence for prokaryotic expression, synthesizing the sequence and ligating it into the vector pET41a to construct pET41a-HIPP7, transforming it into *E. coli*, culturing it in LB liquid medium containing kanamycin, inducing expression with IPTG, isolating and purifying the protein, and preparing a polyclonal antibody against citrus HIPP7 protein by immunizing rabbits with the HIPP7 protein. The polyclonal antibody against citrus HIPP7 protein obtained by this invention can specifically detect citrus HIPP7 protein and can be used to detect the accumulation of HIPP7 protein in different citrus tissues and under different treatment conditions, as well as to detect the accumulation of HIPP7 protein in transgenic plants.

The purpose of the present invention is to provide, as a platform technique that may serve as preparation for pandemics, a rapid antibody production technique in which a non-human animal having a human antibody gene is used. More specifically, the purpose of the present invention is to provide an immunization method with which it is possible to induce, in a short period of time, a high antigen-binding ability of an antibody or a high antibody titer of an antiserum, and a method for producing a human antibody / antiserum with which it is possible to rapidly produce a wide range of neutralizing antibodies.　(1) An immunization method for rapidly inducing a human monoclonal antibody having a high ability to bind to a desired antigen or a human polyclonal antibody having a high antibody titer, the method comprising a step for immunizing a non-human animal a plurality of times with a desired antigen or a nucleic acid encoding the antigen within 30 days from the first immunization, the non-human animal having a human antibody gene or locus, and the non-human animal being such that an endogenous gene or locus of the non-human animal corresponding to the human antibody gene or locus is disrupted or deleted, or mutated so as to result in a loss of expression or low expression. (2) A method for producing a human antibody against a desired antigen using a non-human animal, the method comprising: an immunization step for immunizing the non-human animal with the antigen or a nucleic acid encoding the antigen; a human immunoglobulin-positive B cell isolation step for isolating B cells that are human immunoglobulin-positive from a tissue of the immunized non-human animal; an antibody base sequence acquisition step for acquiring the base sequences of antibody light-chain mRNA-derived cDNA and antibody heavy-chain mRNA-derived cDNA prepared from the isolated B cells; and an antibody production step for producing an antibody on the basis of the acquired antibody base sequence, the non-h

The invention discloses preparation and application of a mouse TMC5 truncated recombinant protein and a polyclonal antibody, and belongs to the technical field of biological medicine. The amino acid sequence of the TMC5 truncated recombinant protein is as shown in SEQ ID NO. 1, and the nucleotide sequence of the TMC5 truncated recombinant protein is as shown in SEQ ID NO. 2. Compared with a full-length protein, the mouse TMC5 truncated recombinant protein provided by the invention has better stability and antigenicity, the obtained polyclonal antibody is high in titer and strong in specificity, the identification of endogenous TMC5 protein in mouse testicular tissues is facilitated, meanwhile, the TMC5 truncated recombinant protein and the polyclonal antibody can also be used for researching a mechanism of regulating spermatogenesis by TMC5, and the application prospect is broad. The blank of preparation of the mouse TMC5 antibody and functional research of the mouse TMC5 antibody in the testis is filled. The mouse TMC5 truncated recombinant protein and the polyclonal antibody can be applied to development of novel diagnostic reagents and drugs for treating male sterility.

The invention relates to a polyclonal antibody of enterohemorrhagic Escherichia coli Intimin protein as well as a preparation method and application of the polyclonal antibody, and the polyclonal antibody of the enterohemorrhagic Escherichia coli Intimin protein can specifically recognize the enterohemorrhagic Escherichia coli Intimin protein and has relatively high titer; according to the present invention, with the enterohemorrhagic Escherichia coli Intimin protein polyclonal antibody, the infection process of enterohemorrhagic Escherichia coli can be deeply understood through the enterohemorrhagic Escherichia coli Intimin protein polyclonal antibody, such that the hereditary basis and the molecular mechanism of the enterohemorrhagic Escherichia coli infection host cell can be further researched; the method has double values for understanding pathogenesis and developing prevention and control means.

This application relates to a label-free immunosensor for Helicobacter pylori, its preparation method, and its application. The method involves directly incubating the Helicobacter pylori multivalent epitope antigen FvpE on a modified electrode, eliminating the need for hybridization labeling during the preparation of the label-free immunosensor. This simplifies the preparation process, making it simple and easy to operate. Furthermore, the prepared label-free immunosensor can directly detect Helicobacter pylori in samples, greatly simplifying the detection process and reducing detection time. Simultaneously, the prepared label-free immunosensor can detect polyclonal antibodies anti-Hp and different antibody genotypes such as CagA, UreA, UreB, and VacA, and can simultaneously detect multiple cytotoxins or virulence factors. This improves the universality of the label-free immunosensor, thereby reducing workload, shortening detection time, and lowering detection costs.

This invention belongs to the field of biotechnology, specifically relating to an anti-human C-reactive protein antibody and its application in the preparation of time-resolved immunofluorescence chromatography quantitative test strips. This application involves pairing and screening the prepared antibody 7A9 with a commercially available antibody (anti-human CRP polyclonal antibody) to obtain the optimal antibody combination. Further optimization of the immunochromatographic reaction system's formulation and process is then performed, ultimately establishing a simple, highly sensitive, and specific dual-antibody sandwich detection system whose performance meets the requirements for clinical sample testing. This system can be used to prepare time-resolved immunofluorescence chromatography quantitative test strips, which exhibit a linear range of 0.5–200 mg / L and a correlation coefficient of [missing value]. R 2 With a coefficient of variation (CV) of ≥0.99 and an intra-batch CV of ≤8%, the test strip offers fast detection speed, completing the test in just 3 minutes. It is suitable for rapid quantitative detection of CRP in clinical emergency and primary healthcare settings, and has promising prospects for industrial application.

This invention relates to an anti-phospholipase A2 receptor polyclonal antibody, its preparation method, and its application. The preparation method includes immunizing animals with phospholipase A2 receptor protein as an antigen and collecting serum; separating and purifying the serum to obtain the anti-phospholipase A2 receptor polyclonal antibody; the amino acid sequence of the phospholipase A2 receptor protein includes the sequence shown in SEQ ID NO.1. This invention designs specific antigens for the preparation of polyclonal antibodies, enabling efficient preparation of polyclonal antibodies that specifically recognize the phospholipase A2 receptor. It has also been found that polyclonal antibodies prepared using eukaryotically expressed antigens, compared to those prepared using prokaryotically expressed or peptide-derived antigens, can more stably and efficiently construct an anti-PLA2R-associated membranous nephropathy (PLA2R MN) animal model.

The invention discloses a litopenaeus vannamei Vg polyclonal antibody as well as a preparation method, a quantitative detection method and application thereof, and relates to the field of biotechnology and immunodetection. The polyclonal antibody is obtained by immunizing a polypeptide antigen containing an amino acid sequence SEQ ID NO: 1 (CPTKYEIETEGEKV), and the polyclonal antibody can specifically recognize the litopenaeus vannamei Vg. The invention provides and establishes a novel non-double antibody sandwich enzyme-linked immunosorbent assay (ELISA) method for the first time, and the core of the novel non-double antibody sandwich enzyme-linked immunosorbent assay (ELISA) method is that a Litopenaeus vannamei VgR ligand binding structural domain is used as a solid-phase capture molecule, and the polyclonal antibody is used as a detection antibody to form a receptor-ligand-antibody detection mode. The method effectively avoids the technical bottleneck that paired antibodies are difficult to prepare in a traditional method, and has the advantages of wide linear range and good repeatability.

This invention discloses a kit for the quantitative detection of C-reactive protein (CRP) in giant pandas and its application, belonging to the field of biodetection technology. The kit disclosed in this invention comprises reagent R2 with the following concentrations: 50 mM PBS pH 7.5, Tween-20 6 ml / L, 270 nm polystyrene carboxylated latex 2.5 g / L, sucrose 30 g / L, BSA 10 g / L, rabbit anti-giant panda CRP polyclonal antibody 6.75 mg / L, and preservative 0.5 ml / L. The rabbit anti-giant panda CRP polyclonal antibody is obtained by immunizing giant white rabbits with the recombinant antigen expressed by the sequence shown in SEQ ID NO. 4. This invention provides a highly efficient, simple, low-cost, and scalable detection method.

The invention discloses a C-reactive protein rapid detection method, and belongs to the technical field of C-reactive protein detection.The C-reactive protein rapid detection device comprises a disc provided with a first liquid storage bin, a second liquid storage bin and a reaction bin, and the first liquid storage bin and the second liquid storage bin are communicated with the reaction bin through corresponding microfluidic channels; a mixed solution of polystyrene microspheres, Tween20, sodium azide, a phosphate buffer and a C reactive protein polyclonal antibody is added into the first liquid storage bin; a mixed solution of a phosphate buffer solution with the pH value of 7.4, Tween 20, polyethylene glycol, sodium chloride and sodium azide is added into the second liquid storage bin; adding a to-be-detected sample into the second liquid storage bin; the disc is driven to rotate; the flow (such as starting, stopping and path switching) of the solution in the micro-channel can be accurately controlled by driving the disc to rotate without externally connecting a pump, a valve and other complex mechanical devices and adjusting the rotating speed of the disc, uniform mixing of a sample and a solution reagent is promoted, the mixing efficiency is remarkably improved compared with passive diffusion, and the reaction is sufficient.

The invention discloses a method for preparing a high-titer polyclonal antibody from a Zika virus strain, which comprises the following steps: selecting the Zika virus strain, culturing in cells, and purifying virus particles with high purity to prepare an immunogen; an optimized animal immunization strategy is adopted and comprises the steps of selecting a proper host animal, applying a potent immunologic adjuvant, namely a Freund's adjuvant, and designing a scientific immunization program, so that humoral immune response of the host is stimulated to the maximum extent; when the titer of the antibody reaches a peak value, collecting antiserum, synthesizing hybridoma cells through splenocytes, and obtaining the high-purity polyclonal antibody through a multi-step purification technology. According to the method, high-purity and non-chemically-inactivated whole virus particles which retain natural conformation are used as immunogens, a mode of primary immunization and multiple booster immunization is adopted, a Freund's adjuvant is combined, animals are continuously stimulated to generate high-titer antibodies, non-specific antibodies and impurities are effectively removed through multi-step purification, and the immunogen immunogen is obtained. The target antibody with high purity and high specificity is obtained.

The invention discloses a kit for quantitatively detecting panda C-reactive protein and application of the kit, and belongs to the technical field of biological detection. The invention discloses a kit for quantitatively detecting panda C reactive protein. A reagent R2 is prepared from the following components in concentration: 50 mM PBS (Phosphate Buffer Solution) with pH (Potential of Hydrogen) of 7.5, 6 ml / L of tween-20, 2.5 g / L of 270 nm polystyrene carboxyl latex, 30 g / L of sucrose, 10 g / L of BSA (Bovine Serum Albumin), 6.75 mg / L of rabbit anti-panda CRP (C reactive protein) polyclonal antibody and 0.5 ml / L of preservative; the rabbit anti-panda CRP polyclonal antibody is obtained by immunizing a rabbit with a recombinant antigen expressed by the sequence as shown in SEQ ID NO.4. The invention provides a detection scheme which is efficient, simple, convenient, low in cost and capable of being popularized on a large scale.

The invention provides a fusion antibody of group A rotavirus, a detection test strip and a kit, and belongs to the technical field of virus detection. The invention provides a fusion antibody of a group A rotavirus. The fusion antibody is obtained by chemically coupling, modifying, purifying and separating a group A rotavirus monoclonal antibody or a group A rotavirus polyclonal antibody. The invention also provides a brand new microsphere labeling method, a test strip for detecting rotavirus is prepared based on the microsphere labeled fusion antibody, the detection sensitivity can reach 100%, the specificity reaches 100%, the accuracy rate is improved by 10 times, and the sensitivity is improved by 30 times. The invention further provides an immunochromatography kit capable of rapidly detecting the rotavirus, the detection speed is high, the detection result can be obtained within 1-15 min, operation is easy, the price is low, and the immunochromatography kit is suitable for popularization and self-detection.

The invention discloses colloidal gold chromatography test paper and a kit for detecting platelet CD36 antigen, the colloidal gold chromatography test paper comprises a bottom plate, a sample pad, a gold-labeled combination pad, a nitrocellulose membrane and a water absorption pad, the gold-labeled combination pad is coated with a colloidal gold labeled mouse anti-human CD36 IgG2a monoclonal antibody and a colloidal gold labeled quality control rabbit IgG polyclonal antibody; the nitrocellulose membrane is provided with a detection line coated with a human platelet CD36 antigen capture antibody and a quality control line coated with quality control goat anti-rabbit polyclonal antibody molecules. When the kit is used for detecting the CD36 antigen, a detected sample is fully split by the platelet lysis buffer and then is added into the sample adding hole of the kit, and a detection result can be observed after waiting for 10-15 minutes. By adopting the detection mode provided by the invention, the CD36 antigen on the human platelets can be quickly, simply, conveniently, sensitively and specifically detected.

The present application relates to the technical field of biotechnology, and particularly relates to a monoclonal antibody against CopGFP and application thereof, the monoclonal antibody can recognize CopGFP protein, and the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; the light chain variable region has three complementarity determining regions with the amino acid sequences shown in SEQ ID NO:1-SEQ ID NO:3; and the heavy chain variable region has three complementarity determining regions with the amino acid sequences shown in SEQ ID NO:8-SEQ ID NO:10. The monoclonal antibody against CopGFP of the present application can specifically recognize CopGFP protein, and the antibody has good specificity and high titer. The monoclonal antibody against CopGFP of the present application has good recognition specificity for in-vitro purified human CopGFP protein and intracellular exogenous expression CopGFP protein; compared with a polyclonal antibody, the monoclonal antibody has stronger recognition specificity.

This invention relates to the field of biochemical technology, and particularly to highly specific haptens of polymyxin B and colistin, artificial antigens, their preparation methods, and applications. The haptens have structures as shown in Formula I or Formula II. The applications include: (1) preparing specific antibodies against polymyxin B or colistin; (2) detecting specific antibodies against polymyxin B or colistin; and (3) preparing reagents for detecting specific antibodies against polymyxin B or colistin. This invention further provides artificial antigens obtained by conjugating the haptens with carrier proteins. Using the artificial antigens provided by this invention as immunogens to immunize experimental animals, highly specific and sensitive antiserum can be prepared, which can then be used to extract and prepare polyclonal antibodies. These methods can be used to establish highly specific, highly sensitive, simple, and rapid detection methods for polymyxin B and colistin, and have significant application value.