126 results about "Vibrio parahemolyticus" patented technology

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

The invention provides a lactobacillus pentosus and a preparation method and application of bacteriocin of the lactobacillus pentosus. The name of the strain is lactobacillus pentosus YCL31, and the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the preservation address is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 66589. The bacteriocin separated and purified from the metabolite of the bacterium can destroy the morphological structure of the vibrio parahemolyticus and inhibit the toxicity of the vibrio parahemolyticus, can also achieve the effect of remarkably inactivating the vibrio parahemolyticus in a metapenaeus ensis system, can effectively delay the spoilage of the metapenaeus ensis, and has a good application prospect as a bacteriostatic agent or preservative. Therefore, development and application of the novel bacteriocin have important reference value in related fields.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention relates to the field of microbiological detection, in particular to a method for detecting vibrio parahaemolyticus by combining RPA-CRISPR / Cas12a with a microfluidic biosensor. According to the method, the preloaded freeze-drying reagent is combined with centrifugally driven liquid operation, so that rapid and closed system detection is realized, and the pollution risk is effectively reduced. According to the invention, quantitative detection of vibrio parahaemolyticus is realized in a centrifugal micro-fluidic chip by systematically optimizing an RPA amplification primer and crRNA. The detection method shows high specificity and high sensitivity to non-target vibrio and other food-borne bacteria, and the lower detection limit is 6.08 copies / mu L. In a word, the invention provides a rapid, portable and sensitive platform, can realize quantitative detection in a wide linear range, provides a practical tool for real-time monitoring of pathogens, and has application prospects in monitoring of aquaculture biosafety and marine product safety.

The invention relates to an extraction method and application of hippophae rhamnoides leaf polyphenol, and belongs to the technical field of food functional component extraction. According to the method, the hippophae rhamnoides leaf polyphenol is extracted by innovatively adopting a wall-breaking-step extraction system combining enzymatic pretreatment with ultrasonic induction, and the method is specifically characterized in that hippophae rhamnoides leaf powder is used as a substrate, and normal-temperature enzymolysis wall-breaking treatment is performed. Furthermore, a sound field is combined to promote non-thermal dissociation of secondary metabolites in the hippophae rhamnoides leaf powder, and a continuous process of'normal-temperature enzymolysis of cellulase-ultrasonic extraction 'is constructed. Then, adding an ethanol solution, and placing in a water bath for reaction extraction; and centrifugally freeze-drying to obtain the crude polyphenol of the sea buckthorn leaves. An antibacterial experiment shows that the hippophae rhamnoides leaf polyphenol crude extract has a remarkable antibacterial effect on food-borne pathogenic bacteria such as vibrio parahaemolyticus and aeromonas hydrophila, the technical scheme effectively keeps the biological activity of polyphenol, meanwhile, the solvent consumption and the oxidative degradation rate of heat-sensitive components are lower than those of a traditional process, and the method is suitable for development of natural preservatives and functional foods.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention discloses a nano antibody for detecting vibrio parahaemolyticus as well as a preparation method and application thereof, and aims to provide a nano antibody for detecting vibrio parahaemolyticus, which is high in detection accuracy, strong in specificity and simple in obtaining method. The amino acid sequence of the nano antibody is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. Belongs to the technical field of biological detection.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.

The invention belongs to the technical field of pathogenic microorganism screening, and particularly relates to vibrio parahaemolyticus capable of causing prawn vibrio vitreovibriosis and application of the vibrio parahaemolyticus, and the pathogenic bacteria can be used for preparing inactivated vaccines or used as the pathogenic bacteria to screen medicine components with the effect of preventing and treating the prawn vibrio vitreovibriosis or a culture method. The preservation number of the vibrio parahaemolyticus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The pathogenicity of the vibrio parahaemolyticus with high pathogenicity and strong lethality is about 1000 times stronger than that of the vibrio parahaemolyticus which is collected by the world animal hygiene organization and causes acute hepatopancreatic necrosis, and the strain is popular in main prawn culture areas and causes serious harm to the prawn fry culture industry in China. The vibrio parahaemolyticus provided by the invention can be used as a positive control for preparing a diagnosis product for the prawn vibrio vitreovirus disease; or preparing an immune vaccine after inactivation.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

The invention belongs to the technical field of microorganisms, and discloses a bifidobacterium animalis subsp. Lactis XB02 strain capable of inhibiting pathogenic bacteria and improving inflammation, the preservation number of the bifidobacterium animalis subsp. Lactis XB02 strain is CGMCC (China General Microbiological Culture Collection Center) No.34942, and the preservation date is June 18, 2025. Meanwhile, the probiotic composition adopting the bacterial strain XB02 also comprises a lactobacillus rhamnosus XB01 bacterial strain. The XB02 strain disclosed by the invention has excellent bacteriostatic activity, especially for vibrio parahaemolyticus and gardneria; the atopic dermatitis symptom can be obviously improved; the intestinal mucosa permeability is reduced, and the intestinal barrier function is enhanced; and when the strain is combined with the XB01 strain for use, a synergistic sleep promoting effect is shown. The invention further provides a probiotic composition containing the strain, a preparation and application of the probiotic composition and the preparation in food, medicine or health care products.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The invention belongs to the technical field of gene engineering, and discloses bacteriophage lysozyme as well as a preparation method and application thereof. The bacteriophage lysozyme provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 4 or a variant fragment with at least 80% homology with the fragment as shown in SEQ ID NO: 4. The antibacterial spectrum of the bacteriophage lysozyme covers a plurality of bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas putida, vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus, and the bacteriophage lysozyme has the advantages of excellent antibacterial performance, high thermal stability and high acid and alkali tolerance, and can be used for preparing the antibacterial bacteriophage lysozyme. The antibacterial agent can be well applied to the fields of food, aquaculture, animal husbandry and the like.

The invention discloses a modified chitosan compound with a function of promoting healing of seawater soaking wounds. The modified chitosan compound DGCS prepared by the invention has good biological activity, has a remarkable promotion effect on cell proliferation, and is enhanced along with the increase of concentration; the inflammation level in cells can be reduced; the healing of a seawater soaking wound can be promoted. An antibacterial experiment shows that the DGCS has good antibacterial activity on escherichia coli, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa, enterococcus faecalis, vibrio vulnificus and vibrio parahaemolyticus.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

The invention discloses a PtALF-8 gene for coding a portunus trituberculatus anti-lipopolysaccharide factor as well as a protein and application of the PtALF-8 gene, and belongs to the technical field of molecular biology. The nucleotide sequence of the PtALF-8 gene is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor protein obtained by encoding the PtALF-8 gene is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor protein PtALF-8 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control and gene-assisted breeding of portunus trituberculatus, and has potential market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 as well as a protein and application thereof, and belongs to the technical field of molecular biology. The nucleotide sequence of the coding gene PtALF-7 is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor obtained by coding of the coding gene PtALF-7 is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor generated by encoding the gene PtALF-7 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control, gene-assisted breeding and the like of the portunus trituberculatus, and has good market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus is as shown in SEQ ID NO. 2. The invention further discloses application of the nucleic acid aptamer A4-CA2 with high affinity to the vibrio parahaemolyticus in detection of the vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2 in preparation of products for detecting the vibrio parahaemolyticus. The affinity and dissociation constant of the nucleic acid aptamer A4-CA2 and the vibrio parahaemolyticus is 76.5 nM, and the nucleic acid aptamer A4-CA2 has high affinity, does not obviously bind to escherichia coli, staphylococcus aureus, aeromonas salmonicida and bacillus cereus, shows good specificity and is suitable for specific detection of the vibrio parahaemolyticus.

The application discloses a Vibrio parahaemolyticus CJ5 and application thereof, and belongs to the technical field of microorganisms. The Vibrio parahaemolyticus CJ5 provided by the application is separated and screened from workshop water of a sea cucumber breeding base, the strain can efficiently and directionally degrade SC-FUC, the degradation product has a relatively high sulfate retention rate and a relatively concentrated molecular mass distribution, under the conditions that the growth temperature is 30 DEG C, the salinity is 3%, the pH value is 8.0 and the inoculation amount is 3%, the degradation rate of the strain to SC-FUC reaches 61.47%, the sulfate mass fraction of the degradation product is 34.55%, and the relative molecular mass distribution range is within 800 Da, so that the sea cucumber fucoidan with DP 2-5 can be prepared.

The application discloses a broad-spectrum short tail phage PhLNJZ2 targeting multi-drug resistant Vibrio and an application thereof, and belongs to the technical field of microorganisms. The phage PhLNJZ2 is preserved in the China Center for Type Culture Collection on October 20, 2025, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 20252251, and the classification and naming is Vibrio phage PhLNJZ2. The phage is screened from a multi-drug resistant Vibrio alginolyticus clinical isolate which is resistant to more than 7 kinds of antibiotics, exhibits high efficient and broad-spectrum lytic activity on three main waterborne pathogenic Vibrio, i.e., Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio harveyi, and the total lytic rate reaches 81.25%. The application further provides a composition containing the phage, a feed additive and an application of the phage in preparation of a medicine for preventing and controlling waterborne multi-drug resistant and mixed Vibrio disease or an environmental disinfectant.

The application provides a bacteriophage for controlling Vibrio infection, and belongs to the field of veterinary technology. The application designs a recombinant tail chain protein rTC and synthesizes an expression gene of the recombinant tail chain protein rTC through amino acid sequence alignment and structure analysis of tail chain proteins of Vibrio phages. The rTC gene is electroporated into Vibrio parahaemolyticus isolated from diseased prawns, and the host bacteria are infected with PHxp1 bacteriophage isolated from seawater; after the rTC gene is recombined with the PHxp1 bacteriophage, the collected bacteriophage is screened with Vibrio alginolyticus to obtain two bacteriophages PHxp1-1 and PHxp1-2 capable of lysing Vibrio alginolyticus; the bacteriophage is screened with Vibrio harveyi to obtain one bacteriophage PHxp1-3 capable of lysing Vibrio harveyi. The host spectrum of the three recombinant bacteriophages is widened. The recombinant tail chain protein bacteriophage greatly reduces the death and growth stagnation of prawns caused by Vibrio infection in prawn farms, and improves the breeding benefit.

The invention discloses an application of nomuraea sp. 18B9 in resisting pathogenic vibrio. The nomuraea sp. 18B9 can be used for resisting pathogenic vibrio. The vibrio is not limited to vibrio candersonii, vibrio mimicus and vibrio parahaemolyticus.

The application discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 and a protein and application thereof, and belongs to the technical field of molecular biology. PtALF-7 The nucleotide sequence of the coding gene is shown as SEQ ID No. 1, and the amino acid sequence of the obtained portunus trituberculatus anti-lipopolysaccharide factor is shown as SEQ ID No. 2. PtALF-7 The in-vitro bacteriostatic experiment proves that the anti-lipopolysaccharide factor coded by the gene has a significant inhibiting effect on the growth of Vibrio parahaemolyticus and Staphylococcus caprae. Therefore, the application lays an application foundation for disease prevention of portunus trituberculatus, gene-assisted breeding and the like, and has good market application value in the development of antibacterial drugs, feed additive production and the like.

The invention belongs to the technical field of biology, and discloses a polypeptide derived from a deep sea ecological environment, an application of the polypeptide in preparation of an antibacterial preparation and an antibacterial drug. According to the application of the polypeptide disclosed by the invention in the preparation of the antibacterial preparation, the polypeptide comprises one or more of amino acid fragments with sequences shown as SEQ ID NO: 1-9, the compound has good bacteriostatic or bactericidal activity on one or more of escherichia coli, halomonas aeruginosa, acinetobacter picoteri, staphylococcus aureus, linear preristeria, klebsiella pneumoniae and vibrio parahaemolyticus, and has extremely low toxicity on mammalian cells under an effective concentration. The compound has excellent bacteriostatic activity, bacteriostatic broad-spectrum property and biocompatibility, and has very excellent application prospects in multiple fields such as food preservation, agricultural disease prevention and control, feed additives and the like.

The invention discloses separation and application of a vibrio parahaemolyticus lytic bacteriophage vBVpaSR27Z (Vibrio parahaemolyticus lytic bacteriophage). The preservation number of the bacteriophage vBVpaSR27Z is GDMCC No: 67438-B1 (GDMCC No: 1). The bacteriophage is separated from an environmental sample by adopting a double-layer plate method, the head of the bacteriophage is in a regular icosahedron shape (84 nm * 71 nm), the tail length of the bacteriophage is about 141 nm, the incubation period of the bacteriophage is only 20 min, the lysis speed is high, and the activity of the bacteriophage is stable within the pH range of 5-10 and the temperature range of 4-37 DEG C; the genome of the vibrio bacteriophage is double-stranded DNA without known drug resistance and toxicity genes, phylogenetic analysis shows that the vibrio bacteriophage has low homology with known vibrio bacteriophages, and the vibrio bacteriophage belongs to a novel genetic resource. The vBVpaSR27Z can rapidly inhibit the growth of strains such as vibrio parahaemolyticus, is suitable for green prevention and control of diseases such as vibrio parahaemolyticus in aquaculture, and can be used as an active component of a biological antibacterial preparation.

The invention discloses five food-borne pathogenic bacteria LAMP method high-throughput detection primer groups, a kit and application, and belongs to the technical field of food-borne pathogenic bacteria detection. The primer group corresponds to five bacteria, namely salmonella, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157: H7 and staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No.1-30. A matched system can complete the whole-process detection at the constant temperature of 62 DEG C for 45 minutes, the result does not need a complicated instrument, the positive grey and negative purple are judged by naked eyes, and the operation is simple and convenient; the detection sensitivity reaches 1-75 copies / reaction, an artificially polluted aquatic product sample does not need to be subjected to enrichment and is subjected to simple splitting decomposition and enrichment, the specificity is high, and no cross reaction exists; the anti-interference capability is outstanding, the method adapts to complex matrixes of aquatic products, and the detection coincidence rate is high; the device is simple in structure and low in cost, only needs a conventional constant temperature device, is suitable for basic food, agriculture, disease control, customs and other detection institutions, food enterprises, on-site law enforcement and on-site detection and other scenes, and provides an efficient and economic technical means for aquatic product safety management and control.