70 results about "Vibrio parahemolyticus" patented technology

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention relates to the field of microbiological detection, in particular to a method for detecting vibrio parahaemolyticus by combining RPA-CRISPR / Cas12a with a microfluidic biosensor. According to the method, the preloaded freeze-drying reagent is combined with centrifugally driven liquid operation, so that rapid and closed system detection is realized, and the pollution risk is effectively reduced. According to the invention, quantitative detection of vibrio parahaemolyticus is realized in a centrifugal micro-fluidic chip by systematically optimizing an RPA amplification primer and crRNA. The detection method shows high specificity and high sensitivity to non-target vibrio and other food-borne bacteria, and the lower detection limit is 6.08 copies / mu L. In a word, the invention provides a rapid, portable and sensitive platform, can realize quantitative detection in a wide linear range, provides a practical tool for real-time monitoring of pathogens, and has application prospects in monitoring of aquaculture biosafety and marine product safety.

The invention discloses a nano antibody for detecting vibrio parahaemolyticus as well as a preparation method and application thereof, and aims to provide a nano antibody for detecting vibrio parahaemolyticus, which is high in detection accuracy, strong in specificity and simple in obtaining method. The amino acid sequence of the nano antibody is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. Belongs to the technical field of biological detection.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

The invention belongs to the technical field of microorganisms, and discloses a bifidobacterium animalis subsp. Lactis XB02 strain capable of inhibiting pathogenic bacteria and improving inflammation, the preservation number of the bifidobacterium animalis subsp. Lactis XB02 strain is CGMCC (China General Microbiological Culture Collection Center) No.34942, and the preservation date is June 18, 2025. Meanwhile, the probiotic composition adopting the bacterial strain XB02 also comprises a lactobacillus rhamnosus XB01 bacterial strain. The XB02 strain disclosed by the invention has excellent bacteriostatic activity, especially for vibrio parahaemolyticus and gardneria; the atopic dermatitis symptom can be obviously improved; the intestinal mucosa permeability is reduced, and the intestinal barrier function is enhanced; and when the strain is combined with the XB01 strain for use, a synergistic sleep promoting effect is shown. The invention further provides a probiotic composition containing the strain, a preparation and application of the probiotic composition and the preparation in food, medicine or health care products.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

The invention discloses a PtALF-8 gene for coding a portunus trituberculatus anti-lipopolysaccharide factor as well as a protein and application of the PtALF-8 gene, and belongs to the technical field of molecular biology. The nucleotide sequence of the PtALF-8 gene is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor protein obtained by encoding the PtALF-8 gene is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor protein PtALF-8 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control and gene-assisted breeding of portunus trituberculatus, and has potential market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 as well as a protein and application thereof, and belongs to the technical field of molecular biology. The nucleotide sequence of the coding gene PtALF-7 is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor obtained by coding of the coding gene PtALF-7 is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor generated by encoding the gene PtALF-7 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control, gene-assisted breeding and the like of the portunus trituberculatus, and has good market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus is as shown in SEQ ID NO. 2. The invention further discloses application of the nucleic acid aptamer A4-CA2 with high affinity to the vibrio parahaemolyticus in detection of the vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2 in preparation of products for detecting the vibrio parahaemolyticus. The affinity and dissociation constant of the nucleic acid aptamer A4-CA2 and the vibrio parahaemolyticus is 76.5 nM, and the nucleic acid aptamer A4-CA2 has high affinity, does not obviously bind to escherichia coli, staphylococcus aureus, aeromonas salmonicida and bacillus cereus, shows good specificity and is suitable for specific detection of the vibrio parahaemolyticus.

The application discloses a Vibrio parahaemolyticus CJ5 and application thereof, and belongs to the technical field of microorganisms. The Vibrio parahaemolyticus CJ5 provided by the application is separated and screened from workshop water of a sea cucumber breeding base, the strain can efficiently and directionally degrade SC-FUC, the degradation product has a relatively high sulfate retention rate and a relatively concentrated molecular mass distribution, under the conditions that the growth temperature is 30 DEG C, the salinity is 3%, the pH value is 8.0 and the inoculation amount is 3%, the degradation rate of the strain to SC-FUC reaches 61.47%, the sulfate mass fraction of the degradation product is 34.55%, and the relative molecular mass distribution range is within 800 Da, so that the sea cucumber fucoidan with DP 2-5 can be prepared.

The application provides a bacteriophage for controlling Vibrio infection, and belongs to the field of veterinary technology. The application designs a recombinant tail chain protein rTC and synthesizes an expression gene of the recombinant tail chain protein rTC through amino acid sequence alignment and structure analysis of tail chain proteins of Vibrio phages. The rTC gene is electroporated into Vibrio parahaemolyticus isolated from diseased prawns, and the host bacteria are infected with PHxp1 bacteriophage isolated from seawater; after the rTC gene is recombined with the PHxp1 bacteriophage, the collected bacteriophage is screened with Vibrio alginolyticus to obtain two bacteriophages PHxp1-1 and PHxp1-2 capable of lysing Vibrio alginolyticus; the bacteriophage is screened with Vibrio harveyi to obtain one bacteriophage PHxp1-3 capable of lysing Vibrio harveyi. The host spectrum of the three recombinant bacteriophages is widened. The recombinant tail chain protein bacteriophage greatly reduces the death and growth stagnation of prawns caused by Vibrio infection in prawn farms, and improves the breeding benefit.

The invention discloses an application of nomuraea sp. 18B9 in resisting pathogenic vibrio. The nomuraea sp. 18B9 can be used for resisting pathogenic vibrio. The vibrio is not limited to vibrio candersonii, vibrio mimicus and vibrio parahaemolyticus.

The application discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 and a protein and application thereof, and belongs to the technical field of molecular biology. PtALF-7 The nucleotide sequence of the coding gene is shown as SEQ ID No. 1, and the amino acid sequence of the obtained portunus trituberculatus anti-lipopolysaccharide factor is shown as SEQ ID No. 2. PtALF-7 The in-vitro bacteriostatic experiment proves that the anti-lipopolysaccharide factor coded by the gene has a significant inhibiting effect on the growth of Vibrio parahaemolyticus and Staphylococcus caprae. Therefore, the application lays an application foundation for disease prevention of portunus trituberculatus, gene-assisted breeding and the like, and has good market application value in the development of antibacterial drugs, feed additive production and the like.

The invention belongs to the technical field of biology, and discloses a polypeptide derived from a deep sea ecological environment, an application of the polypeptide in preparation of an antibacterial preparation and an antibacterial drug. According to the application of the polypeptide disclosed by the invention in the preparation of the antibacterial preparation, the polypeptide comprises one or more of amino acid fragments with sequences shown as SEQ ID NO: 1-9, the compound has good bacteriostatic or bactericidal activity on one or more of escherichia coli, halomonas aeruginosa, acinetobacter picoteri, staphylococcus aureus, linear preristeria, klebsiella pneumoniae and vibrio parahaemolyticus, and has extremely low toxicity on mammalian cells under an effective concentration. The compound has excellent bacteriostatic activity, bacteriostatic broad-spectrum property and biocompatibility, and has very excellent application prospects in multiple fields such as food preservation, agricultural disease prevention and control, feed additives and the like.

The invention discloses separation and application of a vibrio parahaemolyticus lytic bacteriophage vBVpaSR27Z (Vibrio parahaemolyticus lytic bacteriophage). The preservation number of the bacteriophage vBVpaSR27Z is GDMCC No: 67438-B1 (GDMCC No: 1). The bacteriophage is separated from an environmental sample by adopting a double-layer plate method, the head of the bacteriophage is in a regular icosahedron shape (84 nm * 71 nm), the tail length of the bacteriophage is about 141 nm, the incubation period of the bacteriophage is only 20 min, the lysis speed is high, and the activity of the bacteriophage is stable within the pH range of 5-10 and the temperature range of 4-37 DEG C; the genome of the vibrio bacteriophage is double-stranded DNA without known drug resistance and toxicity genes, phylogenetic analysis shows that the vibrio bacteriophage has low homology with known vibrio bacteriophages, and the vibrio bacteriophage belongs to a novel genetic resource. The vBVpaSR27Z can rapidly inhibit the growth of strains such as vibrio parahaemolyticus, is suitable for green prevention and control of diseases such as vibrio parahaemolyticus in aquaculture, and can be used as an active component of a biological antibacterial preparation.

The invention discloses five food-borne pathogenic bacteria LAMP method high-throughput detection primer groups, a kit and application, and belongs to the technical field of food-borne pathogenic bacteria detection. The primer group corresponds to five bacteria, namely salmonella, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157: H7 and staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No.1-30. A matched system can complete the whole-process detection at the constant temperature of 62 DEG C for 45 minutes, the result does not need a complicated instrument, the positive grey and negative purple are judged by naked eyes, and the operation is simple and convenient; the detection sensitivity reaches 1-75 copies / reaction, an artificially polluted aquatic product sample does not need to be subjected to enrichment and is subjected to simple splitting decomposition and enrichment, the specificity is high, and no cross reaction exists; the anti-interference capability is outstanding, the method adapts to complex matrixes of aquatic products, and the detection coincidence rate is high; the device is simple in structure and low in cost, only needs a conventional constant temperature device, is suitable for basic food, agriculture, disease control, customs and other detection institutions, food enterprises, on-site law enforcement and on-site detection and other scenes, and provides an efficient and economic technical means for aquatic product safety management and control.

The invention relates to the technical field of microorganisms, and provides a plant lactobacillus strain which is separated from an intestinal tract sample of wild fish in a Danjiangkou reservoir. Experiments prove that the strain has relatively high acid resistance and bile salt tolerance, and has certain bacteriostatic ability to vibrio parahaemolyticus, vibrio alginolyticus, aeromonas veronii, aeromonas cainea and aeromonas hydrophila. The invention also provides an aquatic feed composition comprising such a strain. The aquatic feed composition containing the DY-JY3 strain can promote growth of largemouth black bass, improve immunity and digestive enzyme activity and adjust intestinal flora, so that the aquatic feed composition has remarkable potential in the aspect of being a probiotic candidate strain in aquaculture application, and a new microbial intervention strategy is provided for anti-infection treatment of fishes. The strain and the composition provided by the invention have wide application prospects in the field of aquatic products.

This invention discloses a bivalent anti-Vibrio parahaemolyticus nanobody, its preparation method, a self-sandwich ELISA kit, and a detection method. The aim is to provide a bivalent anti-Vibrio parahaemolyticus nanobody, kit, and detection method with high accuracy, specificity, and sensitivity for detecting Vibrio parahaemolyticus. The amino acid sequence of the bivalent anti-Vibrio parahaemolyticus nanobody is shown in SEQ ID NO.7. This invention belongs to the field of biodetection technology.

The invention relates to the technical field of microorganisms, and particularly discloses a streptomyces rust red strain JQ021 and application thereof. The strain is preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number of the strain is CCTCC NO: M 20252186. The strain JQ021, a culture of the strain JQ021 and a metabolic extract produced by the strain JQ021 show efficient and broad-spectrum inhibitory activity on various aquatic pathogenic vibrios, especially vibrio parahaemolyticus, vibrio alginolyticus and vibrio Ewana. The invention also relates to a microbial preparation and an antibacterial composition containing the strain or the metabolic extract, and an application of the microbial preparation and the antibacterial composition in preparation of a preparation for preventing and treating aquaculture vibriosis. The strain provides valuable microbial resources for developing safe and efficient antibiotic substitutes.

The application discloses five kinds of high-throughput detection primer groups, kits and application of LAMP method for foodborne pathogenic bacteria, and belongs to the technical field of foodborne pathogenic bacteria detection. The primer groups correspond to five kinds of bacteria, including Salmonella, Vibrio parahaemolyticus, Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No. 1-30. The complete process detection can be completed at 62 DEG C constant temperature for 45 minutes, the result does not need complex instruments, the positive gray and the negative purple are judged by naked eyes, and the operation is simple. The detection sensitivity reaches 1-75 copies / reaction, the artificially contaminated aquatic product sample does not need to be subjected to bacterial enrichment, is subjected to simple lysis and enrichment, has strong specificity and no cross reaction. The anti-interference ability is outstanding, the complex matrix of aquatic products is adapted, the detection coincidence rate is high, the cost is low, only a conventional constant temperature device is needed, and the application is suitable for multiple scenes, such as detection institutions, food enterprises and on-site law enforcement on-site detection of primary food, agriculture, disease control, customs and the like, and provides an efficient and economical technical means for aquatic product safety control.

The invention discloses an application of Schimannella sp. DY612-18-2 for resisting pathogenic vibrio and a preparation method of the Schimannella sp. DY612-18-2. The vibrio is not limited to vibrio harveyi, vibrio mimicus and vibrio parahaemolyticus.

The invention discloses an antibacterial peptide as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO: 1. According to the invention, the antibacterial peptide with high antibacterial activity is designed through an AI technology, a multi-copy (four-copy) recombinant plasmid expression system (pHBM905M-Eel-AMP-4C) based on a pHBM905M vector is constructed, and the antibacterial peptide is efficiently secreted and expressed in pichia pastoris; besides, the antibacterial peptide has excellent thermal stability and pH tolerance, and has a remarkable inhibition effect on common pathogenic bacteria (such as aeromonas hydrophila and vibrio parahaemolyticus) in aquaculture, so that the antibacterial peptide has an important application value in green prevention and control of aquaculture diseases.

The application belongs to the technical field of disease prevention and treatment of aquaculture, and particularly relates to application of honokiol combined with sulfisoxazole sodium in preparation of a Vibrio parahaemolyticus inhibitor, characterized by that the mass ratio of honokiol to sulfisoxazole sodium in the composition is 1:0.5-2, the concentration of honokiol in the composition is 32-64 µg / mL, and the concentration of sulfisoxazole sodium in the composition is 32-128 µg / mL, and the application further provides a medicine for improving sensitivity of Vibrio parahaemolyticus to sulfisoxazole sodium and treating acute hepatopancreas necrosis disease of prawns, and the mass ratio of honokiol to sulfisoxazole sodium in the medicine is 1:0.125-0.5; the composition can significantly reverse drug resistance of Vibrio parahaemolyticus to SMM-Na, reduce the use amount of SMM-Na, and produce a rapid sterilization effect through combined action, and meanwhile, the composition exhibits excellent protection efficacy in prawn bodies.

The invention discloses a spore surface display vibrio parahaemolyticus VPOmpK-MeL recombinant bacillus subtilis as well as a construction method and application thereof. According to the recombinant bacillus subtilis, on the basis of an integrated plasmid pDG364, a recombinant vector which takes spore capsid protein CotY as anchoring protein and displays a fusion fragment of a VPOmpK antigen and bee venom peptide Melittin on the surface is constructed, recombinant protein is converted into a wild type bacillus subtilis 168 gene, and the obtained recombinant spore expressing OmpK-MeL protein has genetic stability; the bacterium directly displays antigen protein on the surface of a spore, does not need to be broken, and can be directly mixed with materials or drink water to immunize animals, so that the problems of difficult protein purification, tedious injection immunization process and the like are avoided; the spores have good stress resistance, so that the spores are easy to store and transport, and the cost is remarkably reduced; the strain can induce specific immune response, and the fusion expressed MeL plays an excellent role in enhancing immunity, so that a new way is provided for prevention and treatment of VP, and the strain can be applied to development of commercial vaccines.

The present invention provides a use of allicin E for preventing and treating Vibrio parahemolyticus infection in giant freshwater prawns, comprising the step of: adding a compound as shown in formula (I) to giant freshwater prawn feed. This can improve Vibrio parahemolyticus infection in giant freshwater prawns, reduce the mortality rate of giant freshwater prawns, and reduce the threat to food and environmental safety caused by long-term use of antibiotics.

This invention relates to the field of microbial technology, and more particularly to a broad-spectrum Aeromonas hydrophila phage and its applications. The Aeromonas hydrophila phage AhP2505006 is classified as follows: Aeromonas hydrophila Phage AhP2505006, with accession number CCTCC NO: M20251307, exhibits cross-species lytic activity. It can effectively lyse *Aeromonas*, *Vibrio parahaemolyticus* and *Vibrio alginolyticus*, and *Citrobacter freundii*. It also possesses excellent targeting ability against multidrug-resistant strains and broad host spectrum characteristics, achieving a lysis rate of up to 80% against multidrug-resistant *Aeromonas hydrophila*. In the prevention and control of diseases caused by various pathogens, or in practical scenarios facing the limitations of traditional antibiotics and highly specific bacteriophages, it has become a highly promising solution.