The invention relates to a white large-fruited strawberry germplasm innovation method and belongs to the technical field of plant breeding. A cultivated variety 'Toyonoka' of large-fruited strawberries having sweet and sour flavor are used as a female parent, a wild white-fruited yellow-hairy strawberry variety 'white bubble' originally from Yunnan in our country is used as a male parent to perform hybridization, the obtained hybrid generation undergoes colchicine processing and directional selections, such as fruit characteristics and features, and the novel white large-fruited strawberry germplasm is bred. By adopting the novel germplasm 'FB02', obtained fruits are conical, and the whole fruits are uniform and regular. The average fruit weight is about 13.4 g, the longitudinal diameter of each fruit is 3.5 cm, and the transverse diameter of each fruit is 2.7 cm. The fruits have white surfaces and low glossiness. Fruit pulp and cores are white. The sweet and sour flavor is agreeable, the fragrance is heavy, and the fruits have rich peach fragrance and moderate hardness. The growth vigor of plants is moderately strong, plant shapes are semi-upright, and the plant height is about 30 cm. Leaves are subround, large and thick, and leaf color is green. Flowers are moderately large, and the crown diameter is about 2.8 cm. The plants are similar to the Toyonoka in resistance, are quite resistant to anthracnose and can be used as a parent material for white strawberry breeding.