87 results about "Directional selection" patented technology

A pipette guide for a standard well plate provides improvements over conventional guides. The improvements include: reference characters to signify a columns of wells exposed, an aperture for viewing those characters, a angled slot and open ended isolator for improved ergonomic function and visibility, a multilevel slide to help prevent contamination and a bi-directional selection assembly for selecting a 96 or 384 well plate.

The invention provides a breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS, which is characterized in that, distant hybridization is performed by taking common wheat variety Xiaoyan 6 as female parent and relative plant rye variety German White as male parent; seeds are acquired after immature embryo salvation culture, colchicine half root soaking method and chromosome doubling; backcross seeds are acquired after disease resistance identification, cytological identification and backcrossing utilizing the original parent Xiaoyan 6 as male parent; then after disease resistance identification and cytological identification, individual plants with excellent comprehensive characters are selected in direction for self crossing, and after continuous self cross selective breeding for six generations, the finally acquired individual plant is confirmed to be wheat-rye T2BL.1RS translocation line after identified by continuous total genome in situ hybridization and three-probe multicolor fluorescence in situ hybridization. The breeding method for the germ plasm of a new translocation line wheat-rye T2BL.1RS has the advantages that, the wheat-rye T2BL.1RS translocation line has high resistance to stripe rust, current prevalent fungus strain, powdery mildew and other important virus strains; the invention has excellent high yield character; the method lays an excellent germplasm base for the cultivation of breakthrough new wheat variety with disease resistance and high yield.

The invention relates to a breeding method of typically sterile non glutinous rice widely compatible sterile line with high stigma exposure rate, belonging to the field of rice breeding. In the invention, indica type temperature sensitive sterile line Peiai 64S serves as a female parent, conventional non glutinous rice variety Xiushui 110 serves as a male parent, the two are hybridized, direction selection is carried out on later generations; single plant with good agronomic trait, high stigma exposure rate and early blossom time is selected, test cross is carried out on the single plant by typically sterile non glutinous rice sterile line Chunjiang 16A dwarfing-sterile, meanwhile, widely compatible testing seeds are used to carry out wide compatibility appraisal, thus breeding fine material Chunjiang 95 with high stigma exposure rate, early blossom time, good wide compatibility and capacity of staying sterile; Chunjiang 16A dwarfing-sterile / Chunjiang 95 F1 serves as the female parent, and Chunjiang 95 serves as the male parent to carry out back cross; fertility appraisal in a generation by generation manner and continuous back cross is carried out on the later generations after the back cross to transform the later generations after the back cross into typically sterile non glutinous rice widely compatible sterile line Chunjiang 95A with high stigma exposure rate and early blossom time, corresponding maintenance line thereof is Chunjiang 95B. The method of the invention has the advantages of improved fertility stability and hybrid non glutinous rice seed production output of non glutinous rice sterile line.

The invention discloses a method for breeding high-yield high-oil peanut species. In the method, a genetic relationship of the selected high-yield high-oil peanut species is found out by an SSR (Simple Sequence Repeat) molecule marker technology; the species with distant hybrid advantages and larger selection difference is used as a parent for preparing cross combination; the variation frequency is expanded by radiation effect, and the selecting range of a high-yield high-oil gene is expanded; the genetic linkage of an adverse gene is broken down by applying hybridization, and the recombination, polymerization and complementation of high yield, high oil and resistance are realized; an individual plant is directionally selected by using ideal characters as a target in stable generation; and the individual plant is inbred for one generation, a plant system with complete and ideal characters is further selected, and the high-yield high-oil plant system is obtained. The invention has the characteristics of rapidly breeding the high-yield high-oil peanut new species and having simple method and high operability.

The invention relates to a breeding method and application of a cytoplasmic male sterility restoring line of brassica napus rapeseed and radish. The method comprises the following steps of: choosing rapeseed S1628 with the preservation number of CCTCC NO: P201203, performing continuous inbreeding directional selection for six years to breed an excellent, high-oil and stable inbred line; using the cytoplasmic male sterility restoring line of the brassica napus rapeseed and radish as a female parent and using the bred excellent and stable inbred line as a male parent to select out strains comprising radish cytoplasmic male sterility restoring genes from large amounts of filial generations; using the strains comprising the radish cytoplasmic male sterility restoring genes as the female parent and using Zhongshuang No.2, Huashuang No.3, Huyou 17, Zunyou No.1 and Zheyou 758 of double-low winter rapeseed varieties in China as recurrent male parents to hybridize, performing the continuous inbreeding directional selection for six years to breed the cytoplasmic male sterility restoring line of the double-low and high-oil brassica napus rapeseed and radish. The restoring line has been crossed with hybrid varieties. A gap of producing the hybrid varieties by the cytoplasmic male sterility restoring line of the double-low radish in China is filled, and the application prospect is broad.

The present invention provides a process for rapidly breeding novel strains of colorful silkworm cocoons, particularly a process for breeding novel strains of natural colorful silkworm cocoons by utilizing selection of gene expression of multi-phenotype special character tags and quickening homozygosity of characters of offspring from wide cross of cultivated silkworms and wild silkworms. Based on the expressive characteristics of a plurality of appreciable morph character stigmata genes (such as ovum color, mottled grain of larvas, skin redness of larvas, volume color of scalewings, color of cocoon fibers, etc.) of offspring from wide cross of cultivated silkworms and wild silkworms at different development phases for the same generation, the wide cross color incunabulum strain syngen is constructed with the goal of precocity, easy cultivation, strong disease resistance, high yield, high feeding efficiency, thin titer and colourful cocoon fibers; the genetic amelioration of multi-phenotype tag character assistant selection and economic character orthoselection is carried out continuously for many years to stabilize the homozygosity of each system character from wide cross of silkworms; simultaneously, desired practical characters of silkworms can be sufficiently recombined and converged to obtain novel strains of natural colorful silkworm cocoons.

A method and apparatus for passive determination of target data (R, K, V) associated with a target from measured and estimated bearing angles. The measured bearing angles (4) are determined by a sonar receiving installation (2) by directionally selective reception of sound waves which are emitted or sent from a target, and the estimated bearing angles are determined from estimated positions of the target. The bearing angle differences from estimated and measured bearing angles are minimized iteratively over a plurality of processing cycles, and a position of the target, on which the minimum is based, is determined as the optimized solution (12). Furthermore, limit values (10) are defined for the target data (R, K, V) to be determined, in order to exclude unrealistic target data as solutions. However, because the optimized solution (12) from the optimization method (6) does not converge with the actual solution if the limit values (10) are chosen erroneously, an error handling process (14) for identification and correction of incorrect limit values (10) is carried out.

The invention discloses a T12 talent evaluation method and a T12 talent evaluation system. The system comprises a talent evaluation subsystem, an interface management module, a personal center module and a report management module. The interface management module comprises a registration management submodule and realizes system operation through registration and login; after registration is completed, automatic entry is realized, personal information is generated, and a personal database is generated through the personal center module; the talent evaluation subsystem is internally provided with multiple modules for evaluation and realizes the evaluation through standard data comparison and directional selection; and after the evaluation is completed, reports with different standards are generated from personal evaluation data through the report management module. The system and method provided by the invention help enterprises to put talents at right posts, improve the efficiency of the enterprises and enhance the competitiveness of the enterprises. At the same time, the talents can find jobs suitable to their career development by means of the system, the overall happiness of the society is improved, and a harmonious social is realized in a real sense.

The invention relates to a breeding method for a plant, in particular to a breeding method for broccoli, belonging to the technical field of plant cultivation. The breeding method comprises the following steps of: performing continuous selfing and directional selection for at least five generations through collected broccoli material to obtain a self compatible line of the broccoli to serve as a transformation male parent to be hybridized with a cytoplasmic male sterile material; breeding a cytoplasmic male sterile line with superior comprehensive performance and stable genetic character through backcross transformation of five generations; then hybridizing the cytoplasmic male sterile line with stable genetic character with the superior self compatible line of the broccoli; and performing field repeated identification and screening to obtain superior hybridized combined variety. According to the method, an optimal growth and development environment for the broccoli is created though low-temperature treatment of seeds in coordination with related facilities and equipment; double blossom and seed setting are realized in one year; the breeding progress can be quickened; and the seed quality is improved.

The invention belongs to the biotechnology field, provides an obtaining method of molecular markers related to chrysanthemum drought resistance, and is used for positioning and cloning of a chrysanthemum drought resistance excellent gene and breeding of new chrysanthemum varieties. The obtaining method comprises the steps: a, identification of the drought resistance; b, analysis of a chrysanthemum variety group structure; c, analysis of chrysanthemum variety genetic relationship; and d, determination of the molecular markers closely related to the chrysanthemum drought resistance. The method simultaneously adopts three molecular marker techniques comprising SRAP, SCoT and EST-SSR, the principles are different with each other and each have pertinency, and obtained dyeing strips are good in polymorphism and high in repeatability, can cover the entire genome, have rich number of strips and are beneficial for obtaining the molecular markers closely related to the drought resistance. 8 molecular markers related to the chrysanthemum drought resistance are totally obtained, early selection, directional selection and accurate selection of excellent drought-resistant varieties are achieved, the work load can be reduced, and the selection efficiency of chrysanthemum drought resistant genotypes is greatly improved, so as to speed up the process of breeding.

The invention belongs to the technical field of corn breeding and particularly relates to a breeding method of a new strain of a sweet-waxy recessive gene corn selfing line. The breeding method of thenew strain of the high-quality, multi-resistant and high-reassortant corn selfing line with super-sweet and waxy double-recessivity utilizes a waxy corn strain NB group synthesized by nine excellentwaxy corn materials and a super-sweet and waxy corn added combined flower sweet-waxy 179 hybrid containing a super-sweet gene (sh2 / sh2) as basic materials for line selection, uses a pedigree method and a molecular marker to assist directional selection of selfing single plants having excellent comprehensive characters and containing super-sweet and waxy double-recessive genes and breeds a new cornselfing strain WT1791 having super-sweet and waxy double-recessivity (sh2 / sh2 and wx / wx). The WT1791 obtained through the breeding method has the advantages of appropriate growth period, higher yield, good cooperation property and good comprehensive resistance.

The invention discloses a method for selecting a long-term domestic silkworm pupa new material by crossing domestic silkworms and wild silkworms, which comprises the following steps: 1, transferring long-term pupa genes in wild silkworms into domestic silkworms; 2, integrating various excellent properties; 3, building a new strain; and 4, performing directional selection. The method can breed the new variety which has specific properties and special utilization values, develop new products for silkworm industry, exploit new consumption fields for the silkworm industry, deeply develop and comprehensive utilize resources of silkworm industry, extend industrial production chain and obtain high added value.

The invention provides a breeding method for a hybrid rice three-line male sterile line with a recessive marker. The recessive marker used by the method is a multi-awn marker and specifically comprises the steps that: 1) a material with the multi-awn marker is used to hybridize with a non-multi-awn material, a generation F1 is expressed to be non-multi-awn, the multi-awn plants and the non-multi-awn plants of a generation F2 are separated according to a ratio of 1:3, and fertile parents with the multi-awn markers are bred through multi-generation directional selection; and 2) the multi-awn fertile plants obtained in the step 1 are taken as male parents which hybridize with the non-multi-awn three-line male sterile line, sterile plants are selected from the generation F1, the sterile plants selected from the generation F1 continuously back-cross with the paternal multi-awn fertile plants, thoroughly abortive individual plants with the multi-awn marker are selected from generations after multi-generation back-crossing and finally the rice three-line male sterile line with the recessive marker is bred. The method provided by the invention has the advantages that the purity of rice seeds can be detected and abnormal plants in a paddy field can be identified intuitively, conveniently, rapidly and accurately.

The invention belongs to the technical field of crop disease-resistance genetic breeding, and provides a wheat anti-gibberellic in-vitro directional selection breeding method, which comprises: (1) Fusarium graminearum strain separation and culture; (2) crude toxin obtaining; (3) hybridization F1 generation seed obtaining; (4) resistant microspore arrhenokaryon development; (5) resistant callus differentiation; (6) haploid seedling obtaining; (7) anti-gibberellic strain obtaining; and (8) anti-gibberellic characteristic identification on the doubled haploid individual so as to screen the stable anti-gibberellic strain line. According to the present invention, the toxin is used to stress the wide microspore genotype variation and massive microspore cell populations due to gene recombination in the F1 generation anther tissue, the sensitivity difference of the resistant genotype microspore and the non-resistant genotype microspore on toxin is used to screen the resistant genotype, the selection characteristic is directional, the selected groups are large, and the selection efficiency is high.

The invention provides a breeding method capable of resisting wheat yellow mosaic disease, and relates to the technical field of a crop breeding method. According to the breeding method, Ningmai 9 which is high in yellow mosaic disease resistance and high in excellent character combining ability is taken as a male parent, and Ningmai 8 or Yangmai variety which is high in yield and wide in adaptation and has the yellow mosaic disease is taken as a female parent, The method comprises the steps of carrying out hybridization on the male parent and the female parent to obtain F1; breeding the F1 to produce F2, F3 and F4, selecting excellent single spikes of the F2 and the F3, and carrying out blend threshing; selecting excellent single spikes of the F4, separating the spikes for threshing, and sowing head progeny rows F5 in a disease nursery; carrying out wheat yellow mosaic disease resistance identification on the F5, and selecting the head progeny rows which are high in resistance and good in agronomic trait; then, sowing the harvested head progeny rows in the disease nursery to form spike lines F6; repeatedly identifying the disease resistance of all the spike lines, and selecting the excellent spike lines; sowing the excellent spike lines of the F6 to form an evaluation nursery, and selecting the good lines which are disease-resistant, good in quality and high in yield. According to the breeding method, excellent genetic recombination and additive effects which are supplemented by disease resistance, agronomic trait, quality character and the like are used for directional selection of multiple generations, so that Ningmai 16 and a batch of budding new lines which are disease-resistant, good in quality and high in yield are cultured; the new lines are high in yellow mosaic disease resistance and has the advantages of having scab resistance and being high in yield potential, prominent in special quality, and the like, thus having production application values.

The invention relates to a white large-fruited strawberry germplasm innovation method and belongs to the technical field of plant breeding. A cultivated variety 'Toyonoka' of large-fruited strawberries having sweet and sour flavor are used as a female parent, a wild white-fruited yellow-hairy strawberry variety 'white bubble' originally from Yunnan in our country is used as a male parent to perform hybridization, the obtained hybrid generation undergoes colchicine processing and directional selections, such as fruit characteristics and features, and the novel white large-fruited strawberry germplasm is bred. By adopting the novel germplasm 'FB02', obtained fruits are conical, and the whole fruits are uniform and regular. The average fruit weight is about 13.4 g, the longitudinal diameter of each fruit is 3.5 cm, and the transverse diameter of each fruit is 2.7 cm. The fruits have white surfaces and low glossiness. Fruit pulp and cores are white. The sweet and sour flavor is agreeable, the fragrance is heavy, and the fruits have rich peach fragrance and moderate hardness. The growth vigor of plants is moderately strong, plant shapes are semi-upright, and the plant height is about 30 cm. Leaves are subround, large and thick, and leaf color is green. Flowers are moderately large, and the crown diameter is about 2.8 cm. The plants are similar to the Toyonoka in resistance, are quite resistant to anthracnose and can be used as a parent material for white strawberry breeding.

The invention provides a method for breeding a storage-resistant, high-yield and good-quality new green Chinese onion variety. Qingye No.1 green Chinese onion serves as the material, a green Chinese onion sterile line breeding nursery is built, a green Chinese onion male sterile line and a maintainer line are bred, and a good-quality green Chinese onion male sterile line assessment and screening system is built; single plant selfing is conducted in green Chinese onion resources with different characters, a stable and homozygous male parent line is directionally selected and bred, comprehensive character identification is conducted, and a good male parent line is bred; by hybridizing the bred good male sterile line and the male parent line, a hybrid combination is prepared, and the new green Chinese onion variety is bred by identifying storage resistance, yielding ability and stress resistance of offspring of the hybrid combination. An efficient male sterile line breeding technical assessment and screening system is built for the first time, germplasm resources are innovated, the new triple crossing green Chinese onion variety which is resistant to storage, high in yield, resistant to diseases, high in stress resistance and suitable for multiple-season cultivation is bred, and technical support is provided for breeding of good-quality new green Chinese onion varieties.

The invention discloses a breeding method for rapidly obtaining a cytoplasm male sterility line and a maintenance line of carmine radish. The method mainly comprises the following steps: selecting different types of carmine radish materials, performing first generation selfing, determining a selective breeding object, performing orientation selection on a single plant, and performing second generation selfing; performing orientation selection on the single plant for continuously selfing in a selfing offspring group, and performing orientation selection on the single plant according to the selective breeding object; selecting cytoplasm male sterility as a female parent, selecting the selected single plant as a male parent, performing interspecies cross, obtaining hybrid F1, thereby transferring CMS genes of the sterility to collard; continuously selfing and purifying the selfing offspring, and screening a sterile plant which is the closest to the selective breeding object of the male parent to serve as a female parent in a cytoplasm male sterility hybrid plant group, taking the screen single plant which is the closest to the selective breeding object in the selfing offspring as a male parent for continuously performing paired cross; and purifying the selfing offspring, transferring the male sterility, simultaneously performing paired cross on each generation, and selfing multiple generations to obtain the maintenance line and the male sterility line. According to the method, the labor and cost can be obviously reduced, and the breeding time is shortened.

A method for selectively breeding the rape variety with yellow seeds and high oil content features that different yellow-seed rape varieties are hybridized with the karyon male-sterility plant in the recurrent selection group of created cytoplamic male sterility restoring line to create the recurrent selection group of cytoplasmic male sterility restoring line, and recurrent selection is performed to selectively breed the cytoplasmic male sterility restoring line of said rape with yellow seeds and high oil content. Its advantages are simple process, low cost, continuous directional selection, and high effect.

The invention relates to a method for directionally and selectively separating intestinal bacteria, and belongs to the technical field of microbial separation. The invention provides a method for directionally and selectively separating intestinal bacteria. The method comprises the following steps: before separating samples, performing high-throughput sequencing on samples and samples growing in different culture media; selecting samples with high abundance content of required strains through bioinformatics standardized analysis; and mastering the requirements of nutritional ingredients and the interaction relationship among the bacteria so as to increase or reduce corresponding nutrient substances and bacterial fermentation liquor, and performing directional separation to obtain the required strains.

The invention relates to a method for selecting a low-laying-rate hen in a feeding process of cage culture laying hens. The method comprises the following steps: dividing a plurality of groups of hens in a hen group, recording the egg laying quantity of each group for successive days, calculating the laying rate per day of each group, and calculating the group average laying rate of a group which does not achieve 100 percent laying rate; taking a group of which the group average laying rate is lower than a certain value as an alternative elimination group; observing the egg laying situations of individual hens in the alternative elimination group within several days; if the egg laying quantity of a certain hen within individual observation days is zero, eliminating the hen. According to the method provided by the invention, traditional selection of the low-laying-rate hen is converted from experience-based selection to data-based selection, and mass selection is transformed into small-scale directional selection, so that the accuracy rate, the efficiency and the convenience in hen selection are remarkably improved, not only a great number of feeding costs and expenses are reduced in time, but also a potential epidemic disease risk on other healthy hens in the whole hen group caused by a small number of hens in a disease status or a sub-health status is remarkably reduced.

The invention discloses an elevator oil-free self-lubricating guide shoe. The elevator oil-free self-lubricating guide shoe comprises a guide shoe base and is characterized in that the guide shoe baseis provided with a U-shaped clamping groove, a damping mechanism is arranged in the clamping groove and comprises a groove frame with a U-shaped mounting groove, and shoe liners matched with an elevator guiding rail are arranged in the U-shaped mounting groove; multi-dimensional damping balls are arranged on the outer side of the U-shaped mounting groove of the groove frame; and each shoe liner comprises an ultra-high-molecular-weight polyethylene liner body and grease mixed and stored in the liner body. Oil adding devices such as an oil cup on the guide shoe are omitted, thus the structure is simplified, the problems that oil contamination is serious and the top-layer / bottom-pit occupying space is large are solved, and range limitation of guide shoe directional selection is eliminated; awhole guide whip achieves overall force bearing and integrated damping, effective damping is obtained in stereo, multi-dimensional and multi-angle modes, and particularly, the phenomenon of sudden orwavy abnormal vibration can be coped with; and the structure is reasonable and compact, and replacement and maintenance are convenient.

The invention provides a breeding method for a novel peanut variety. The breeding method comprises the following steps: with a high-yield disease-resistant line 025 spring / 26 as a female parent, hybridizing the female parent with a high-yield disease-resistant variety Shanyou 162 and carrying out F1-F4 continuous screening so as to breed a high-yield disease-resistant strain (025 spring / 26 * Shanyou 162) F5; with sweet osmanthus 22 as a female parent, hybridizing the female parent with 01 autumn / 813 and carrying out F1-F4 continuous screening so as to breed a high-yield high-oil high-kernel-percent excellent strain 3001 / 18; and with 3001 / 18 as a female parent, hybridizing the female parent with the (025 spring / 26 * Shanyou 162) F5 and carrying out F1-F9 continuous screening so as to breed a high-yield high-oil disease-resistant novel peanut variety sweet osmanthus 33. The breeding method for the novel peanut variety is simple and highly efficient; and the bred novel peanut variety has the advantages of a large per-plant-fruiting number, high yield, high oil and disease resistance and is especially applicable to cultivation and plantation in Guangxi Province, China.

The invention belongs to the agricultural technology, and relates to the field of crop genetics and breeding. The invention relates to a rapid directional improvement technology for wheat target traits. The rapid directional improvement technology comprises the following steps of 1, hybridizing a receptor variety which does not contain a target trait gene and is used as a female parent and a donor wheat variety which contains the target trait gene and is used as a male parent to generate F1 seeds; carrying out backcross on the F1 seeds and receptor varieties to generate BC1 seeds; carrying out backcross on the selected single plant carrying the target trait gene and the receptor variety to generate BC2 seeds; and 2, planting the BC2 seeds in a greenhouse, carrying out KASP marker-assisted selection and whole genome background selection, and carrying out backcross on the selected single plant carrying the target trait gene and the receptor variety to generate BC3 seeds and the like. By means of the technology, in the wheat breeding process, rapid and accurate directional selection and directional improvement can be achieved on target traits, the new variety breeding period is greatly shortened, and the new variety breeding efficiency is improved.

The invention discloses a few-mode fiber-based high-order mode Brillouin optical fiber laser. The laser is of an annular cavity structure and comprises a narrow-line wide pump laser, an optical amplifier, a first optical fiber mode selection coupler, a first polarization controller, an optical fiber circulator, a second optical fiber mode selection coupler, a second polarization controller and a few-mode fiber. The first optical fiber mode selection coupler is an extra-cavity mode conversion device, and can realize the directional selection coupling of a fundamental transverse mode in a single-mode fiber and a specific high-order mode in a few-mode fiber. The second optical fiber mode selection coupler is an intra-cavity mode coupling device, and can realize the directional selection coupling of a high-order mode in a few-mode fiber and a high-order mode in a few-mode fiber. Based on the Brillouin nonlinear gain of the few-mode fiber in the annular cavity, the resonance amplification of the high-order mode in the cavity is achieved. The high-order mode laser is directly outputted.

The invention provides a method for breeding shattering-resisting high-yield hybrid rice. The method comprises the following steps: an easy-shattering two-line sterile line Guangzhan 63S is adopted as a female parent, and a difficult-threshing excellent self-fertile restorer line is adopted a male parent; hybridization is carried out; and directional selection is carried out among the offspring, such that a shattering-resisting temperature-sensitive sterile line 5303S is bred. According to the sterile line, shattering resistance is dominant. A hybrid F1 bred with the sterile line is shattering resistant. The sterile line can be used as a hybrid rice seed production female parent, such that hybrid rice seed production yield loss can be reduced. With the shattering-resisting excellent sterile line breed, mature-stage rice shattering can be effectively reduced, and yield loss caused by adverse weather influence and during harvesting processes can be effectively reduced. The breed is suitable for existing paddy rice cultivation method and mechanized harvesting. With the method, hybrid paddy rice seed production cost is reduced, farmer income increase is ensured, and food security of our nation is guaranteed.

The invention discloses a method for preparing a high-oleic oilseed rape material by utilizing artificially synthesized brassica napus. The method comprises the steps of utilizing the artificially synthesized brassica napus for hybridizing with natural brassica napus, obtaining a great deal of variations through mass exchange and recombination among filial generation chromosomes, and deficiency, insertion, transposition and inactivation of gene sequences, preventing brassica napus oleic acid synthesis from transforming to linoleic acid and linolenic acid, and utilizing mutation accumulation to acquire a high oleic acid material. According to the method provided by the invention, in earlier generation, oleic acid characteristics have no need to be selected, so that the breeding period can be shortened through a generation-adding technique; in earlier generation, a planting group contains about 300 plants (strains), so that compared with thousands of single plants (strains) for mutation breeding, the work load is smaller; during orientation selection, the selective pressure is increased by successive generations, so that the frequency of acquiring different types of high oleic acid is increased, and the breeding efficiency is improved. According to the method provided by the invention, the breeding selection group is small, the work load is small, the variation range is large, and high oleic acid brassica napus genetic resources with the oleic acid content being higher than 80 percent are obtained.

The invention provides a molecular identification marker for fertile cytoplasm of clustery tabasco pepper and a maintainer line breeding method. The method disclosed by the invention comprises the following steps: cultivating etiolated seedlings of fine varieties of clustery tabasco pepper by utilizing seeds, extracting mitochondria DNA, and screening varieties with fertile cytoplasm genotype N according to a molecular marker Mt_InDel10 in the invention; finally, performing flowering period hybridization on 100% of male sterility line materials and listed varieties with the fertile cytoplasm genotype N, rapidly breeding the male sterility line and maintainer line with excellent apparent characters according to fertility segregation conditions of hybrid offspring, and providing parent stockfor the male sterility three-line conveyance crossbreeding breeding. According to the breeding method disclosed by the invention, molecular marker screening and the conventional crossbreeding are combined, the blindness of the conventional breeding is reduced, the breeding age limit is shortened, the directive selection effect is improved, the breeding process is accelerated, and the molecular identification marker has important application value for accelerating improvement and promotion of excellent local varieties of the clustery tabasco pepper.

The invention relates to a completely-artificial hemibagrus wyckioides reproduction method. According to the method, individuals with remarkable growth rate advantages are selected from F1 progenies obtained through wild parent reproduction to serve as reserved parent fish, gender distinguishing is conducted after the reserved parent fish is over two years old, and then superior individuals are selected for cultivation and taken as artificial parent fish after being bred to over three years old; individuals from different wild parent combinations are selected from the artificial parent fish to serve as male and female parent fish for completely-artificial reproduction; odinagogue is injected into the female parent fish, and odinagogue is also injected into the male parent fish when odinagogue is injected into the female parent fish for the second time; dry artificial insemination is conducted by dissecting the male fish to take out testes and acquire seminal fluid and conducting egg stripping on the female fish, and then incubation and fry cultivation are conducted by routine. By the adoption of the method, the wild parents can be protected, cost for obtaining the wild parent fish can be reduced, labor force is saved remarkably, reproduction efficiency is improved, individuals with good growth traits can be selected in a targeted mode for reproduction, genetic diversity is maintained, and the problems of inbreeding and germplasm resource degradation during conventional reproduction are solved.