The invention discloses a greenhouse psammophyte culture medium. Four different matrix layers, namely a base layer, an original soil layer, a nutritive layer and a covering layer, are sequentially paved from bottom to top, wherein the base layer is one or a mixture of more than one of quartz, feldspar, mica, kaolinite, limestone and granite, the thickness of the base layer is 30-80cm, and particle size is 1-5cm; the original soil layer is obtained by mixing chestnut soil, gray desert soil and desert sandy soil in volume ratio of (1-2):10:(1-3), and the thickness of the original soil layer is 20-60cm; the nutritive layer is a mixture of organic matters, inorganic minerals, inorganic fertilizer, microelement fertilizer and microbial fertilizer, and the thickness of the nutritive layer is 20-100cm; and the covering layer is formed by vermiculite and / or volcanic rock, and the thickness of the covering layer is 5-10cm. The greenhouse psammophyte culture medium is easy to produce and low in cost and can obviously improve the transplanting survival rate of greenhouse landscape psammophytes when applied to cultivation of the greenhouse landscape psammophytes.