The application discloses a method for preparing rice with changed
tiller number, and belongs to the technical field of genetic breeding. The method comprises the following steps: introducing a coding
gene of an OsD14
mutant into target rice to obtain rice with changed
tiller number, wherein the
tiller number of the rice with changed tiller number is higher or lower than that of the target rice, and the OsD14
mutant is a
protein obtained by mutating the 10th, 11th, 13th and 15th amino acids in SEQ ID NO: 1 into other amino acids and keeping other amino acids in SEQ ID NO: 1 unchanged. It is proved by experiments that whether ubiquitination degradation of the OsD14 occurs is related to a
phosphorylation state of the OsD14, the number of rice tillers is adjusted by using the
phosphorylation state of the OsD14, and the
plant type is improved, so that the yield of the rice is improved, and the method has a wide application prospect.