8 results about "Stem elongation" patented technology

The invention relates to the technical field of culture medium manufacturing, in particular to a ford nervilia leaf tissue culture rhizome proliferation culture medium and a preparation method of the ford nervilia leaf tissue culture rhizome proliferation culture medium. The proliferation material is prepared from the following components in parts by weight: 2 to 4 parts of 6-benayl aminopurine, 0.3 to 0.7 part of naphthylacetic acid, 0.3 to 0.6 part of indolebutyric acid, 4 to 6 parts of gibberellin, 95 to 105 parts of novel coconut juice extract, 18 to 22 parts of novel pomegranate extract, 250 to 350 parts of cane sugar, 60 to 70 parts of agar and 3 to 5 parts of activated carbon. The improved pomegranate rind extract is subjected to ultrasonic and purification composite treatment, more antioxidant components such as ellagic acid and gallic acid are released, the antioxidant components, fermentation-enhanced amino acid and a nano silicon / selenium compound in the improved coconut juice extract form a dual antioxidant barrier, free radicals in plant cells are actively removed, oxidative damage is reduced, and the anti-aging effect is achieved. The hormone combination is precisely proportioned, so that lateral bud germination and stem elongation can be promoted.

This invention discloses a peony PsLBD12 Genes and their application in regulating root development PsLBD12 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsLBD12 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsLBD12 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene grew faster, exhibited earlier stem elongation, and showed earlier senescence of basal leaves in the later stages of growth. The taproot length was significantly shortened, the number of lateral roots was significantly increased, and the endogenous hormones IAA and ABA in the leaves were significantly increased. The average number of roots in detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

The invention relates to the technical field of agricultural biology, and discloses an efficient in-vitro regeneration and rapid propagation method for Shannong No.1 rootstocks, and the method comprises the steps of explant selection and pretreatment, sterile disinfection, axillary bud germination and induction, adventitious bud proliferation, rooting culture and acclimatization and transplanting. The browning rate and the pollution rate of the explants are effectively reduced by combining full-dark culture at the initial induction stage; gibberellin A3 is creatively added into a proliferation culture medium to be matched with 6-benzylamino adenine and indole-3-butyric acid, so that a bud seedling vitrification phenomenon is prevented, and stem elongation is promoted; in the rooting stage, dark treatment is combined with high-concentration auxin induction, so that the rooting rate and the root system quality are improved. According to the method, an efficient and stable regeneration system is established, the method has the advantages of high propagation coefficient, robust seedling, high transplanting survival rate and the like, and reliable technical support is provided for large-scale propagation of the Shannong No.1 rootstock.

The invention discloses a method for promoting stem elongation and adventitious bud regeneration of hibiscus mutabilis, and belongs to the technical field of plant tissue culture, and the stem elongation method provided by the invention comprises the step of inoculating the hibiscus mutabilis stem into an MS elongation culture medium added with 5.0 mg.L <-1 > GA3 and 0.5 mg.L <-1 > IAA for culture. The adventitious bud regeneration method provided by the invention comprises the following steps: inoculating a Cottonia mutabilis leaf disc into an MS regeneration culture medium added with 1.0 mg.L <-1 > 6-BA and 0.08 mg.L <-1 > NAA, and culturing. Through a specific plant growth regulator ratio, the method effectively solves the problem of low propagation and transgenosis efficiency caused by height, dwarf and small plant of the hibiscus mutabilis, can significantly promote the elongation of stem segments of the hibiscus mutabilis and induce efficient adventitious bud regeneration, and provides a reliable technical basis for rapid propagation and genetic transformation of the hibiscus mutabilis.

The invention discloses a moso bamboo stalk elongation and drought resistance double-effect regulation gene PeHECT1, and relates to the technical field of plant molecular biology. The PeHECT1 gene overexpression vector is transferred into a japonica rice variety Nipponbare through an agrobacterium tumefaciens-mediated rice mature embryo callus transformation method, and the result shows that compared with a wild type strain, an overexpression strain shows obvious drought resistance under the drought stress condition, the length between stem nodes of the overexpression strain is obviously changed, and the yield of the overexpression strain is obviously increased. The stalk length is obviously shortened. The result provides a theoretical basis for research on moso bamboo stalk elongation and drought resistance, and also provides important resources for rice plant type improvement and drought resistance improvement.

The invention discloses an application of a rapid alkalization factor GmRALF4 gene and an encoded protein thereof in inhibition of shade-induced soybean stalk elongation, which comprises the following steps: amplifying an encoded nucleotide sequence of the soybean rapid alkalization factor GmRALF4 gene, and carrying out carrier recombination by adopting a seamless connection method; the coding nucleotide sequence of the soybean rapid alkalization factor GmRALF4 gene is efficiently cloned to a pBF vector; positive transformed seedlings are obtained through herbicide resistance screening of the transformation receptor endowed with the herbicide-resistant marker gene. The soybean rapid alkalization factor GmRALF4 gene disclosed by the invention has a remarkable inhibition effect on shading-induced soybean stem elongation, the result is accurate and reliable, and the regulation and control method is simple and convenient to operate, can be formulated and is easy to popularize and apply.

The application belongs to the technical field of genetic engineering, and particularly relates to a CmWRKY25 Application of a gene in regulating Clivia flower stem elongation. CmWRKY25 The CDS sequence of the gene is shown as SEQ ID NO. 1. The application first clearly shows that the WRKY family transcription factor CmWRKY25 responds to a salicylic acid signal, and positively regulates the Clivia flower bud differentiation process.

An agricultural drone fertilization device, belonging to the agricultural field, addresses the problem of fertilizer clumps of varying sizes, leading to excessive localized fertilization due to the simultaneous release of clumped and non-clumped fertilizer. In areas of excessive fertilization, crops are prone to nutrient overload, manifesting as thick leaves, excessive stem elongation, soft tissues, and reduced resistance to lodging and pests / diseases, making them susceptible to disease and pest infestations. Conversely, in areas of insufficient fertilization, crops suffer from nutrient deficiency, resulting in slow growth, yellowing leaves, and stunted growth, severely impacting overall crop yield and quality. This agricultural drone fertilization device includes a drone body and a first support plate fixedly connected below the drone body. A rotating mechanism, during its vertical movement, separates clumped fertilizer, resulting in a more even fertilizer distribution compared to existing technologies, thereby promoting better crop growth.