362 results about "Gene targets" patented technology

Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in glnD, wherein said modification is selected from the group consisting of: deletion of the entire gene, deletion of substantially the entire gene, deletion of an ACT domain, deletion of more than 50% of an ACT domain, deactivation of an ACT domain, and deactivation of an UTase domain.

The invention provides a primer probe composition and a kit for detecting a Databean virus and application, and relates to the technical field of biological detection, the primer probe composition comprises a primer probe group aiming at a Databean virus S gene and a primer probe group aiming at a Databean virus L gene, the Databean virus L and S genes are used as amplification target genes for design, and the kit is used for detecting the Databean virus. The primer probe group aiming at the S gene of the Databu virus and the primer probe group aiming at the L gene of the Databu virus are used as the primer probe composition, so that multi-gene target amplification is realized, and the positive detection rate reaches 100% and is higher than the positive detection rate of single-gene amplification; the lowest detection limit is up to 200 copies / mL; the quantification limit reaches 500 copies / mL, and the quantification linear range is 5.0 * 10 < 2 >-1.0 * 10 < 8 > copies / mL. The technical problem that in the prior art, a high-sensitivity and high-accuracy multiplex qPCR detection method for the nucleic acid of the copyda virus is lacked is solved.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The present disclosure provides Crispr / cas9-based repressors for silencing gene targets in vivo and methods of use.

The invention discloses a multi-subtype influenza A virus nucleic acid detection kit and a detection method, and relates to the technical field of medical detection.The kit comprises a group of reverse transcription RT-RPA amplification primer pairs used for amplifying influenza A virus M gene target sequences, the reverse transcription RT-RPA amplification primer pairs are designed for specific areas of subtypes H1N1, H2N2, H3N2, H5N1 and H7N9, and the reverse transcription RT-RPA amplification primer pairs are designed for specific areas of subtypes H1N1, H2N2, H3N2, H5N1 and H7N9; the crRNA library comprises a plurality of crRNA molecules which are respectively complementary with different conserved regions in the M gene target sequence and is used for guiding Cas12a protein to carry out specific recognition; a Cas12a protein reagent; the reporter molecule can be subjected to non-specific cleavage by the Cas12a protein; the enzyme preparation, the buffer solution and the nucleotide substrate are required by RT-RPA reaction. The invention aims to realize synchronous, rapid, high-sensitivity and high-specificity detection of various subtype influenza A viruses.

The invention relates to application of an OsMPK4 gene in enhancing low-temperature stress resistance in a rice seedling stage. Specifically, the rice OsMPK4 gene participating in positive regulation and control of low-temperature stress resistance is cloned in rice, so that the rice OsMPK4 gene is overexpressed, and compared with a wild plant, the rice OsMPK4 gene overexpressed plant shows the low-temperature-resistant characteristic, and the survival rate is remarkably increased. The discovery of the new function of the rice OsMPK4 gene provides a new gene target and resource for improving rice low-temperature stress resistance genetic breeding.

The invention provides a method for screening disease-related gene targets based on whole genome association analysis, and belongs to the technical field of disease genetic risk assessment, the method comprises the following steps: collecting and recording a sample information data set; performing whole genome sequencing on the samples to obtain SNP data, and combining the SNP data of all the samples to obtain a training data set; performing principal component analysis on the SNP matrix, and screening the top 10-30 SNPs as covariables of the model; and carrying out whole genome association analysis on the training data set and constructing a logistic regression model, and screening out the SNP related to the disease according to the P value. According to the method, confounding factors can be effectively controlled by adopting the logistic regression model and introducing covariables (such as age, gender and the number of weekly exercises), high-dimensional data are processed through a regularization method, and the risk of over-fitting is reduced. The method can also capture interaction between SNPs, and provides more accurate disease-related SNP recognition.

The invention relates to the technical field of gene breeding, in particular to an application of FveWRKY50 protein and a coding gene thereof in regulation and control of plant anthracnose resistance, the FveWRKY50 gene is knocked out from strawberries and overexpressed, it is verified that the strawberry FveWRKY50 gene can negatively regulate and control the anthracnose resistance, and by reducing the expression quantity of the FveWRKY50 protein in plants, the anthracnose resistance of the plants can be negatively regulated and controlled. The anthracnose resistance of plants can be effectively improved. The verification of the anthracnose resistance function of the FveWRKY50 gene provides a new gene target and resource for cultivating anthracnose resistance improving plant varieties, and lays a certain theoretical foundation for researching the anthracnose resistance responding mechanism and the adverse environment resisting molecular mechanism of plants.

The invention relates to the technical field of plant genetic engineering, in particular to application of ZmAAAP59 protein and a coding gene thereof in regulation and control of high temperature stress tolerance of corn, the ZmAAAP59 gene is knocked out in corn, it is determined that a mutant of the ZmAAAP59 gene has an obvious high temperature resistance phenotype, it is verified that the corn ZmAAAP59 gene can negatively regulate and control plant heat resistance, and by reducing the expression quantity of the ZmAAAP59 gene in the plant, the corn ZmAAAP59 gene can negatively regulate and control plant heat resistance. The heat resistance of plants can be effectively improved. The discovery of the heat-resistant function of the ZmAAAP59 gene provides a new gene target and resource for cultivating heat-resistant plant varieties, has important significance on research on a heat-resistant molecular mechanism of corn, and lays a certain theoretical foundation for research on a high-temperature stress response mechanism and a molecular mechanism for resisting adverse environments of plants.

The invention discloses application of amantadine in inhibition of ITGAV in preparation of medicines for treating dry age-related macular degeneration, and belongs to the technical field of biological medicines. At present, no report for researching the ITGAV gene in the dry AMD exists, the specific action mechanism is not clear, and amantadine adaptation diseases do not include the dry AMD; according to the application disclosed by the invention, the gene target ITGAV is screened from mutual hair generation of microglial cells and RPE cells in a retina microenvironment, and amantadine is used for inhibiting ITGAV protein to relieve the progress of AMD, so that a new thought is provided for treating dry AMD. Experiments show that amantadine can inhibit ITGAV and delay EMT transformation of RPE cells. In a dry AMD mouse model induced by sodium iodate, RPE cell damage can be remarkably relieved by intraocular injection of amantadine, and the structure and function of a retina layer are improved. These results indicate that it may function in early intervention of dry AMD.

1. The name of the product of this design: Fully automatic nucleic acid detection analyzer (iGenebox 3A). 2. Purpose of the product of this design: used for the detection of nucleic acids of multiple pathogenic pathogens and human gene target nucleic acids. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention relates to an application of a soybean GmWRKY75 gene in improvement of agronomic characters of soybeans. In particular, the invention relates to application of the soybean GmWRKY75 gene in increasing the yield or the grain number of a single soybean plant and / or in soybean plant type improvement genetic breeding. The recombinant vector and the genetic engineering strain containing the soybean GmWRKY75 gene are applied to increase of single-plant yield or single-plant grain number of soybeans and / or soybean plant type improvement genetic breeding. And a method for increasing the yield of a soybean single plant or the grain number of the single plant and / or soybean plant type improvement genetic breeding by overexpressing the soybean GmWRKY75 gene and the like. Through cloning of the soybean GmWRKY75 gene and discovery of a new function, a new gene target and a new resource are provided for soybean plant type improvement genetic breeding.

The invention discloses application of a substance taking an SAA2 gene as a target spot in preparation of a kit for diagnosing DKD and a medicine for treating DKD, relates to the technical field of detection of gene target spots related to diabetic nephropathy and research and development of targeted medicines, discloses an effect of the SAA2 gene in diagnosis of DKD, and meanwhile, finds that expression of the SAA2 gene in DKD kidney tissues is increased, and the expression of the SAA2 gene in the DKD kidney tissues is reduced. The result is negatively correlated with renal function. The invention also finds that the SAA2 gene is related to podocyte injury and inflammatory response, and can reflect the inflammation level of DKD to a certain extent. The effect of the compound in DKD-mediated inflammatory reaction can be verified through further in-vivo and in-vitro experiments subsequently, and a certain theoretical basis is laid for research and development of targeted drugs.

The invention provides gRNA (guide Ribonucleic Acid) targeting an AGT (Absorption Growth Tube) gene and application thereof, in particular, the invention provides gRNA targeting an AGT gene exon-intron splicing junction region, the invention firstly discovers that the gRNA designed by targeting the AGT gene splicing junction region (such as an intron 1 receptor, an intron 2 donor / receptor and an intron 4 donor / receptor) can effectively inhibit the expression of the AGT gene, therefore, the gRNA can be used for inhibiting the expression of the AGT gene. According to the gRNA designed by the invention, the gene editing efficiency can be remarkably improved; and / or treating a disease.

The invention provides a primer probe combination and a kit for detecting the copy number of a 22q11 region based on digital PCR and a use method of the primer probe combination. Specific probes of a target gene TBX1, a target gene CRKL, a target gene SMARCB1 and a reference gene GAPDH are integrated in different fluorescence channels, and the copy number of the target gene is detected according to the ratio of the copy number of the reference gene to the copy number of the three target genes. According to the invention, amplification products of different sites in different fluorescence channels are integrated in the same system in digital PCR reaction, so that the detection flux is greatly improved, and low-cost and high-flexibility accurate quantification of 22q11. 2 copy number variation is realized through a single-channel multi-target fluorescence layering strategy; and the dependence on expensive equipment, complex operation and professional analysis in the prior art is overcome.

The invention discloses application of a transcription factor ZmbZIP27 in regulation and control of corn plant height and ear height, and belongs to the technical field of plant genetic engineering and corn molecular breeding. The invention reveals that the bZIP transcription factor family member ZmbZIP27 has an important biological function of regulating and controlling the corn plant height and lodging resistance for the first time. A ZmbZIP27 knockout vector is constructed through a CRISPR / Cas9 gene editing technology, and an obtained homozygous mutant strain shows remarkable plant type improvement characteristics compared with a wild type: the plant height is reduced by 8-12%, and the ear height is reduced by 15-22%. And a new gene target and a breeding strategy are provided for creating a new high-yield lodging-resistant corn variety suitable for mechanical harvesting.

The invention discloses a cancer prognosis prediction method and system based on multi-omics fusion. The method comprises the following steps: acquiring multiple groups of omics data; preprocessing the plurality of groups of omics data to obtain a plurality of groups of first omics data features; inputting the plurality of groups of first omics data features into a multi-head attention-based graph convolutional network feature extraction model to obtain a plurality of groups of second omics features; inputting the plurality of groups of second group of characteristics into an attention mechanism fusion module based on biological priori knowledge guidance to obtain fusion characteristics; and inputting the fusion features into a prognostic scoring model to obtain prognostic scores. In addition, potential gene targets related to cancer prognosis are obtained by adopting an omics data feature importance evaluation method. The prognosis prediction conclusion obtained by the method is high in accuracy and high in interpretability.

The invention discloses identification and application of a mycobacterium tuberculosis bacteriophage susceptibility gene, and a gene target which affects mycobacterium bacteriophage infection efficiency is obtained through combined application of a mycobacterium tuberculosis CRISPR-KO library and a CRISPRi library and bacteriophage D29 infection screening. Reagents or drugs targeting phage susceptibility genes or encoded proteins thereof are screened based on the gene targets, and the reagents or drugs can knock out the phage susceptibility genes or inhibit expression of the phage susceptibility genes, or inhibit activity of the encoded proteins of the phage susceptibility genes, serve as phage synergists, or are combined with antibiotics to be used with phages, and can be used for preparing drugs for treating the phage susceptibility genes or the encoded proteins of the phage susceptibility genes. The bacteriophage-antibiotic composition has the advantages that a synergistic sterilization effect is achieved, a new strategy is provided for bacteriophage-antibiotic combined treatment of drug-resistant mycobacterium infection, rapid sterilization is expected to be achieved, the treatment course is shortened, and finally the target of rapid treatment of tuberculosis and other pathogenic mycobacterium infection is achieved.

The invention relates to the technical field of microbiology, in particular to a biological reagent research and development method based on microbial functional gene screening. The method comprises the following steps: acquiring a microorganism sample, and performing genome sequencing extraction treatment and gene transcription analysis to generate transcriptome data of the microorganism sample; obtaining a biological reagent research and development target function, and performing target function gene screening treatment and gene targeted knockout treatment to generate a target function gene targeted knockout experimental group; carrying out gene cloning on the target functional gene targeted knockout experimental group, transferring into a preset host strain, carrying out function expression evaluation analysis to obtain a target functional expression level corresponding to each target functional targeted knockout gene, and carrying out excellent function screening and verification to generate a biological reagent excellent function verification gene, and the method is applied to a research and development process corresponding to a biological reagent so as to generate a target functional biological reagent product. The functional screening efficiency and the specificity of biological reagent products can be improved.

The invention relates to application of a rice OsbZIP83 gene in enhancing low-temperature stress resistance in a rice seedling stage. Specifically, the rice OsbZIP83 gene participating in positive regulation and control of low-temperature stress resistance is cloned in rice, so that the rice OsbZIP83 gene is overexpressed, and compared with a wild plant, the rice OsbZIP83 gene overexpressed plant shows the low-temperature-resistant characteristic, and the survival rate is remarkably increased. The discovery of the new function of the rice OsbZIP83 gene provides a new gene target and resource for improving rice low-temperature stress resistance genetic breeding.

The invention relates to application of rice genes SPPL1 and / or SPPL2 in improving high-temperature resistance of rice. Specifically, rice genes SPPL1 and / or SPPL2 participating in positive regulation and control of high-temperature stress resistance are cloned in rice, so that the rice genes SPPL1 and / or SPPL2 are overexpressed, and compared with a wild plant, the rice gene SPPL1 and / or SPPL2 overexpressed plant shows high-temperature-resistant characteristics, and agronomic characters (such as maturing rate, thousand seed weight, single-plant yield, plot yield and the like) are remarkably improved. Through cloning and discovery of new functions of the rice gene SPPL1 and / or SPPL2, a new gene target and a new resource are provided for improving high-temperature resistance genetic breeding of rice.

The invention discloses an efficient gene editing system for streptomyces as well as a construction method and application of the efficient gene editing system. The system comprises a TnpB nuclease which can be expressed in streptomyces and a guide RNA, wherein the guide RNA comprises an RNA skeleton, a gene targeting section and a gene sequence of hepatitis delta virus (HDV) ribozyme. Wherein the gene targeting segment is located at the 3'end of the RNA skeleton and is a nucleic acid fragment with the length of 12-40bp after a TAM sequence (5 'TTGAT) on a target gene; the hepatitis D virus (HDV) ribozyme is used for stabilizing the RNA skeleton-gene targeting section structure. According to the streptomyces mini-gene editing tool provided by the invention, in the presence of a homologous repair template, the gene editing efficiency can reach 70-100% in streptomyces, and the gene editing efficiency under the action of the guide RNA after rational design can reach 100%.

The invention discloses a pathogen detection kit based on microfluidics and a CRISPR / Cas system and a detection method thereof, and particularly relates to the technical field of biological detection.The pathogen detection kit comprises a nucleic acid releasing agent layer, an RPA reaction chamber layer and a CRISPR detection chamber layer, the nucleic acid releasing agent layer is located above the RPA reaction chamber layer, and the CRISPR detection chamber layer is located above the RPA reaction chamber layer. The RPA reaction chamber layer is positioned above the CRISPR detection chamber layer; a nucleic acid releasing chamber is formed in the bottom surface of the nucleic acid releasing agent layer in a penetrating manner. According to the present invention, the kit has the high specific recognition ability on the specific pathogen nucleic acid sequence, can accurately detect the target pathogen, can reduce the false result occurrence probability, can detect a variety of pathogens or a plurality of gene targets of the same pathogen at one time, and can rapidly amplify the nucleic acid through the optimized RPA reaction system of the RPA reaction chamber so as to substantially shorten the detection time.

The invention relates to an application of an Aspergillus oryzae Aokap9 gene in improving the kojic acid yield of Aspergillus oryzae, which is characterized in that an expression cassette containing an Aspergillus oryzae U6 promoter PU6, an Aokap9 gene target sequence, sgRNA and an Aspergillus oryzae U6 terminator TU6 is constructed by inhibiting the expression or activity of the Aokap9 gene in the Aspergillus oryzae and taking the 278th site to the 297th site of the Aokap9 gene as target regions, and is inserted into a gene editing vector pPRTII-Cas9, so that the Aspergillus oryzae Aokap9 gene is obtained. The method comprises the following steps: generating a gene editing vector pPRTII-Cas9-Aokap9 by using aspergillus oryzae as a raw material, then carrying out protoplast genetic transformation on the aspergillus oryzae, screening strains with high yield of kojic acid, and improving the kojic acid yield of the aspergillus oryzae. According to the invention, the CRISPR / Cas9 technology is utilized to excavate and identify the gene for improving the kojic acid yield of aspergillus oryzae, and the aspergillus oryzae strain with high kojic acid yield is constructed, so that the efficient biosynthesis of the kojic acid of aspergillus oryzae is effectively promoted.

The invention discloses a PRX gene, application thereof and peroxidase protein, and relates to the field of type III peroxidase and application thereof, and the peroxidase protein coded by the PRX gene screened by a dry water database transcriptome can participate in the synthesis process of lignin in citrus peel. The PRX gene CgPRX24 / CgPRX41 / CgPRX65 can participate in the synthesis process of lignin in citrus peel, the hardness of the citrus peel is promoted, and meanwhile, the PRX gene CgPRX24 / CgPRX41 / CgPRX65 can be used as a gene target for genetic engineering and biological preservation of citrus, so that the purpose of improving the peel texture in the postharvest storage process is achieved.

The invention relates to the technical field of biological medicines, in particular to a recombinant oncolytic virus for expressing a bispecific or trispecific single-chain antibody and application of the recombinant oncolytic virus. The recombinant oncolytic virus comprises an antibody gene integrated into a virus skeleton of the recombinant oncolytic virus; the antibody gene comprises a target antibody gene targeting tumor cells and at least one antibody gene targeting immune cells. When the antibody gene comprises a target antibody gene targeting tumor cells and an antibody gene targeting immune cells, the antibody gene is a bispecific antibody; when the antibody gene comprises a target antibody gene targeting tumor cells and two antibody genes targeting immune cells, the antibody gene is a trispecific antibody. According to the present invention, the coding gene having the bispecific single-chain antibody or the trispecific single-chain antibody is integrated in the modified oncolytic virus, such that the gene of the bispecific single-chain antibody or the trispecific single-chain antibody can be expressed in vivo so as to further improve the tumor cell treatment effect.

The invention relates to the technical field of gene engineering, in particular to a cynanchum thesioides CtPDS gene as well as a VIGS virus vector and a silencing method of the cynanchum thesioides CtPDS gene. The invention provides phytoene dehydrogenase of cynanchum thesioides. The phytoene dehydrogenase has an amino acid sequence as shown in SEQ ID NO. 1. The invention discloses an amino acid sequence of cynanchum thesioides phytoene dehydrogenase and a sequence of a coding gene CtPDS of the cynanchum thesioides phytoene dehydrogenase, and provides a target nucleic acid molecule for silencing the CtPDS gene, a VIGS silencing system and a silencing method on the basis of the amino acid sequence of the cynanchum thesioides phytoene dehydrogenase. The leaf CtPDS gene expression level of a CtPDS gene silent plant constructed by the method provided by the invention is remarkably reduced, the silence efficiency reaches 56-65%, the chlorophyll and carotenoid contents are remarkably reduced, the leaf has a whitening phenotype, and a technical support is provided for developing functional research of cynanchum thesioides key genes.

The invention relates to application of a ZmbZIP68 protein and a coding gene thereof in regulation and control of heat resistance of corn. It is found that the corn ZmbZIP68 gene can positively regulate the heat resistance of plants, and the heat resistance of the plants can be effectively improved by overexpressing the expression quantity of the ZmbZIP68 gene. The discovery of the heat-resistant function of the ZmbZIP68 gene provides a new gene target and resource for cultivating heat-resistant plant varieties, has important significance on research on a heat-resistant molecular mechanism of corn, and lays a certain theoretical foundation for research on a high-temperature stress response mechanism and a molecular mechanism for resisting adverse environments of plants.

The invention provides an application of a GABA-A receptor negative regulator in preparation of a medicine for treating pan cancer, and belongs to the technical field of medicines. The research finds that in the tumor microenvironment of panthenic cancer, the GABA-A receptor negative regulator acts on the key cancer-promoting gene target of cancer cells for drug therapy, abnormal overexpression of the key cancer-promoting gene causing cancer occurrence and development can be controlled, the inhibition effect of the cancer cells on immune cells is reduced, and the cancer-promoting effect of the cancer cells is improved. The medicine can be used for treating cancer cells, so that an immunosuppressive tumor microenvironment beneficial to cancer cell growth is destroyed, immune infiltration of the cancer tumor microenvironment is readjusted, a normal immune system of an organism is activated to treat the cancer cells, and the medicine can also be used for reducing NF-kb signal channels of the cancer cells to reduce inflammatory factor expression of the cancer cells and promote apoptosis of the cancer cells. The GABA-A receptor negative regulator acts on key cancer promoting gene targets of cancer cells, so that accurate and effective targeted therapy can be carried out on cancers on the molecular level.

The invention relates to a method for carrying out gene targeting on cephalosporium acremonium based on a CRISPR-Cas9 gene editing system and application. According to the method, cephalosporium acremonium is taken as a starting strain, a ku70 gene is knocked out to improve the homologous recombination probability of exogenous DNA of the strain, and meanwhile, a Cas9 nuclease coding sequence is integrated into a cephalosporium acremonium genome to obtain the recombinant cephalosporium acremonium engineering strain with high homologous recombination efficiency. And further transforming and recombining the cephalosporium acremonium engineering bacteria to perform gene targeting on the target gene DNA by constructing a recombinant vector containing homologous arms on two sides of the gene targeting target DNA sequence, an sgRNA expression box and a resistance selection marker. An efficient cephalosporium acremonium gene targeting platform is constructed, single-gene gene knockout with the homologous recombination efficiency exceeding 90% and deletion of genome large fragments exceeding 30 kb are achieved, and basic support is provided for genetic modification in cephalosporium acremonium.