160 results about "Gene targets" patented technology

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses a multi-subtype influenza A virus nucleic acid detection kit and a detection method, and relates to the technical field of medical detection.The kit comprises a group of reverse transcription RT-RPA amplification primer pairs used for amplifying influenza A virus M gene target sequences, the reverse transcription RT-RPA amplification primer pairs are designed for specific areas of subtypes H1N1, H2N2, H3N2, H5N1 and H7N9, and the reverse transcription RT-RPA amplification primer pairs are designed for specific areas of subtypes H1N1, H2N2, H3N2, H5N1 and H7N9; the crRNA library comprises a plurality of crRNA molecules which are respectively complementary with different conserved regions in the M gene target sequence and is used for guiding Cas12a protein to carry out specific recognition; a Cas12a protein reagent; the reporter molecule can be subjected to non-specific cleavage by the Cas12a protein; the enzyme preparation, the buffer solution and the nucleotide substrate are required by RT-RPA reaction. The invention aims to realize synchronous, rapid, high-sensitivity and high-specificity detection of various subtype influenza A viruses.

The invention relates to application of an OsMPK4 gene in enhancing low-temperature stress resistance in a rice seedling stage. Specifically, the rice OsMPK4 gene participating in positive regulation and control of low-temperature stress resistance is cloned in rice, so that the rice OsMPK4 gene is overexpressed, and compared with a wild plant, the rice OsMPK4 gene overexpressed plant shows the low-temperature-resistant characteristic, and the survival rate is remarkably increased. The discovery of the new function of the rice OsMPK4 gene provides a new gene target and resource for improving rice low-temperature stress resistance genetic breeding.

The present disclosure relates to systems, non-transitory computer-readable media, and methods that analyze gene perturbation machine learning embeddings and clinical observation data sets utilizing machine learning, explainability models, and causal discovery models to generate causal predictions between one or more genes and clinical outcomes. Indeed, in one or more implementations, the disclosed systems identify gene perturbation embeddings generated from cells exposed to perturbations. For instance, the disclosed systems select a cluster of genes from a plurality of genes by applying a clustering model to the gene perturbation embeddings. In some instances, the disclosed systems select gene targets from the cluster of genes by using a machine learning classification model trained on a plurality of features of the clinical observation data set. Moreover, in some instances, the disclosed systems generate the causal prediction from the gene targets and the clinical observation data set utilizing a causal discovery model.

The mechanisms driving the development of extracapillary lesions in focal segmental glomerulosclerosis (FSGS) and crescentic glomerulonephritis (CGN) remain poorly understood. A key question is how parietal epithelial cells (PECs) invade glomerular capillaries, thereby promoting injury and kidney failure. Here the inventors show that expression of the tetraspanin CD9 increases markedly in PECs in mouse models of CGN and FSGS, and in kidneys from individuals diagnosed with these diseases. Cd9 gene targeting in PECs prevents glomerular damage in CGN and FSGS mouse models. Mechanistically, CD9 deficiency prevents the oriented migration of PECs into the glomerular tuft and their acquisition of CD44 and β1 integrin expression. These findings highlight a critical role for de novo expression of CD9 as a common pathogenic switch driving the PEC phenotype in CGN and FSGS, while offering a potential therapeutic avenue to treat these conditions. Accordingly, CD9 represents a reliable biomarker and as well as a biotargets in glomerulonephritides.

The invention discloses an application of an OsCFIm68b gene in regulation and control of Cd resistance of rice and Cd accumulation of grains. The OsCFIm68b gene in rice is knocked out through gene editing to obtain a knockout mutant, and it is found that under the Cd polluted soil culture condition, the length of a main root is shortened, the number of lateral roots is reduced, the length of a total root is shortened, and the Cd concentration of grains is reduced to 1 / 3 of that of a wild type. Therefore, the OsCFIm68b gene is a negative regulatory factor for encoding Cd accumulation of the rice grains, the Cd accumulation of the rice grains can be improved by weakening the expression of the OsCFIm68b gene, the Cd resistance of the rice is improved, and a new gene target is provided for Cd low-accumulation breeding of the rice.

The present application relates to the technical field of genetic engineering, and specifically discloses a Chlamydomonas reinhardtii SnRK2.7 gene and application thereof in adaptation to osmotic stress and maintenance of cell survival. The present application first discloses SnRK2.7 the key biological function of the gene in maintaining Chlamydomonas cell homeostasis. By knocking out the gene through CRISPR / Cas9 gene editing technology and adopting an expression frame driven by an endogenous promoter for genetic back complementation, it is confirmed that SnRK2.7 the gene positively regulates cell osmotic stress tolerance and maintains cell long-term survival. In addition, the present application first discovers that the SnRK2.7 protein is specifically located in the Chlamydomonas expansion vesicle, which provides direct spatial evidence for the cell function. The above discovery not only expands the understanding of the osmotic stress signal transduction mechanism of Chlamydomonas, but also provides a new gene target and research tool for the improvement of microalgae stress adaptability, and has important basic research value and biological technology application potential.

The present application relates to the field of plant genetic engineering technology, in particular to the application of ZmAAAP59 protein and its coding gene in regulating corn tolerance to high temperature stress, by knocking out ZmAAAP59 gene in corn, it is determined that the mutant has obvious high temperature tolerance phenotype, it is verified that corn ZmAAAP59 gene can negatively regulate plant heat resistance, by reducing the expression amount of ZmAAAP59 gene in plants, the heat resistance of plants can be effectively increased.The discovery of the heat resistance function of ZmAAAP59 gene provides a new gene target and resource for breeding heat-resistant plant varieties, has important significance for the study of the heat tolerance molecular mechanism of corn, and lays a certain theoretical foundation for the study of the mechanism of plant response to high temperature stress and the molecular mechanism of resisting adverse environment.

The application belongs to the technical field of biology, and discloses application of CmPHL1 transcription factor in inhibition of citrus leaf oil cell formation and regulation of volatile substances. In view of the problem of insufficient research on existing citrus oil cell formation and essential oil synthesis, a pGBI-CmPHL1 overexpression vector is constructed, and positive strains are obtained by means of Agrobacterium-mediated transformation of citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and the content of monoterpenes, sesquiterpenes and other volatile substances. CmPHL1 targets the molecular regulation network of oil cell development, accurately regulates and is heritable, provides a new gene target for citrus quality improvement and breeding, is in line with the direction of green agriculture, and has important economic and ecological benefits.

This invention discloses a method for preparing a co-loaded dual-gene targeted delivery system and its application, involving the construction and preparation of a dual-gene (pHNF4α, pFOXA3) delivery system. This delivery system can target myofibroblasts (MFs) and activated hepatic stellate cells (aHSCs) and can be used for gene therapy of liver fibrosis. This invention synthesizes the targeting carrier material PEI. 25K -PEG 2K -pPB, through electrostatic adsorption, combines with pHNF4α and pFOXA3 to form a co-loaded dual-gene targeted delivery system, F4α / A3@PP-pPB. This delivery system, through the targeting group pPB, binds to MFs (metacellular matrix cells) that lead to excessive accumulation of extracellular matrix in fibrotic livers and to PDGFR (polydimethylformamide) highly expressed on the surface of aHSCs. On the one hand, it inhibits the activation of aHSCs; on the other hand, it transdifferentiates MFs into hepatocyte-like cells (iHep). These two modes, while combating liver fibrosis, can also achieve a certain degree of liver function reconstruction through transdifferentiated iHep cells, providing new ideas and methods for the effective treatment of liver fibrosis.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

The invention discloses application of a LOXL2 gene in resisting avian leukosis virus infection, and belongs to the technical field of biology. The nucleotide sequence of the LOXL2 gene is as shown in SEQ ID NO. 1. The invention provides a new gene target LOXL2 related to avian leukosis virus infection resistance, and proves the effect of the LOXL2 gene in regulating and controlling avian leukosis virus replication. Replication of the avian leukemia virus can be promoted by inhibiting expression of the LOXL2 gene in a host cell, and replication of the avian leukemia virus can be inhibited by overexpressing the LOXL2 gene in the host cell. According to the invention, favorable theoretical guidance and technical support are provided for the application of the LOXL2 gene as a target in the aspects of development of drugs for treating avian leukemia, breeding of transgenic animals for resisting avian leukemia, construction of avian leukemia virus infection models and the like, and the LOXL2 gene has wide application prospects and extremely high market value.

The invention relates to the field of medical treatment, and discloses a sepsis risk prompt detection method based on model prediction, which comprises the following steps of: collecting a clinical blood sample of an acute infection patient, matching gene expression characteristics of the clinical blood sample with a database through gene target analysis, primarily screening out gene expression data obviously related to sepsis, and comparing the gene expression data with the database; according to the gene expression data of the marked sepsis patient samples and the gene expression data of the non-sepsis patient samples, gene expression differences are obtained, differential gene data and clinical data are established, a machine learning algorithm model is trained, and a sepsis recognition model is obtained; differential gene detection data related to sepsis is input into a sepsis recognition model for prediction, a sepsis recognition result is output, the sepsis recognition result and detection data of a sepsis rehabilitation patient are compared and analyzed, a model prediction effect is evaluated, and early warning is performed on prediction and evaluation results. The accuracy of model prediction is improved, and the prediction capability of the model is enhanced.

The invention relates to a ZmNF-YA13 gene and application thereof in plant disease resistance breeding, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the ZmNF-YA13 gene is shown as SEQ ID NO.1, the amino acid sequence of protein coded by the gene is shown as SEQ ID NO.2, the ZmNF-YA13 gene can be used for regulating and controlling the resistance of plants to corn southern leaf blight, and the ZmNF-YA13 gene can be applied to plant disease resistance breeding. The over-expression of the ZmNF-YA13 gene can enhance the resistance of the plant to the corn southern leaf blight, and the function deletion of the ZmNF-YA13 gene can weaken the resistance of the plant to the corn southern leaf blight. According to the invention, the resistance of the ZmNF-YA13 gene for positively regulating the plant to the corn southern leaf blight is found and verified, and a new gene target and an important theoretical basis are provided for corn breeding for disease resistance.

The invention relates to the technical field of virus detection, and particularly discloses a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for identifying a classical swine fever virus vaccine strain and a wild strain. The kit comprises a specific primer combination, a specific fluorescent probe, a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction solution, a positive control and a negative control, the primer combination comprises a vaccine strain and wild strain specific primer pair targeting a virus 5 '-UTR region and an E2 gene; the fluorescent probes comprise probes corresponding to the targets respectively and are marked with fluorophores; verification is carried out through double gene targets, missing detection caused by single site mutation is effectively avoided, and the method has the advantages of high specificity, high sensitivity and high accuracy; the invention further provides a non-diagnostic purpose detection method of the kit, detection can be completed within 2 h, and the kit is suitable for rapid identification, epidemic situation monitoring and purification effect evaluation of classical swine fever virus vaccine strains and wild strains in clinical samples.

This invention belongs to the field of biological breeding technology, specifically involving SlCSLA2 Application of genes in improving the storage and transportability of tomato fruit. This invention addresses the industry problem of severe storage and transport losses in tomatoes due to excessive softening after harvest. For the first time, it utilizes CRISPR / Cas9 gene editing technology to knock out the cell wall hemicellulose (mannan) synthase gene. SlCSLA2 This invention has led to the creation of a tomato mutant with significantly enhanced fruit firmness. The firmness of the entire fruit during the red-ripe stage is 35%–42% higher than that of the wild type, and the firmness can be maintained for about a week after harvest, effectively delaying the softening process and significantly improving storage and transportability. This invention provides new gene targets and technical approaches for breeding tomato varieties with improved storage and transportability.

The present application relates to a CRISPR-mediated immortalized sheep fetal skin fibroblast cell line in the field of variation or genetic engineering and application thereof.The construction of the CRISPR-mediated immortalized sheep fetal skin fibroblast cell line of the present application comprises the following steps: step one, constructing a Cas9 site-directed knockout plasmid containing an FGF5 gene target sequence; step two, constructing a homologous repair plasmid Donor-2 kb HA-SV40LT containing an FGF5 gene homologous arm; step three, constructing a donor DNA containing an SV40LT protein expression frame; and step four, preparing a sheep fetal fibroblast cell line stably expressing SV40LT protein.The immortalized SFF-FGF5 / SV40LT monoclonal cell strain created by the present application can become an ideal tool cell line for genome editing researches such as sheep CRISPR library screening, multiple gene editing and mutation gene function exploration.

The application provides a HBV inhibitor screening method based on a molecule-gene interaction constraint graph convolution network. In view of the limitation of a traditional drug discovery method in processing complex biological data, the application is based on a constructed compound library verified by anti-HBV in-vitro activity, a plurality of gene targets associated with the corresponding compound and an interaction network thereof, a graph data processing capacity of a graph convolution network model is used, and a molecule-gene interaction constraint graph convolution network model is constructed. The model combines an interaction matrix of a target protein corresponding to the gene, a gene feature matrix and a compound activity label, and effectively predicts the biological activity category of the compound. The specific steps include data processing, graph data generation, graph convolution network model training, hyperparameter optimization and model evaluation. The model parameter AUC value is 0.97, and the model effect is good. The application provides a new path and idea for virtual screening of anti-HBV drugs, and has potential application value.

The invention discloses an application of a CTL-S1 gene and miR-let-7 in regulation and control of reproductive development of bactrocera cucurbitae. The CTL-S1 gene has high specificity expression in bactrocera cucurbitae ovary, and the hatching rate of offspring can be reduced by inhibiting the expression of the CTL-S1 gene through RNA interference. Through the analysis of a dual luciferase report system of RNA co-immunoprecipitation, the result shows that the miR-let-7 and the CTL-S1 have a targeting relationship. Overexpression of miR-let-7 can lead to significant reduction of the expression quantity of the CTL-S1 gene, and a phenotype similar to RNA interference inhibition of the expression of the CTL-S1 gene appears, so that the hatching rate of offspring is reduced. The invention provides a potential gene target for developing a genetic control strategy for destroying the reproduction of bactrocera cucurbitae, provides a new thought for the subsequent biological control of insects by adopting a sterile technology, and has a wide application prospect.

The invention relates to the technical field of plant breeding, in particular to application of ZmCIPK9 protein or a coding gene thereof in regulating and controlling heat resistance of plants. The ZmCIPK9 protein comprises an amino acid sequence as shown in SEQ ID NO. 1 (sequence identifier number 1). 1, and the coding gene comprises a nucleotide sequence as shown in SEQ ID NO. 2. The application comprises the step of improving the heat resistance of the plant by improving the expression level of the ZmCIPK9 protein in the plant or the coding gene of the ZmCIPK9 protein. New functions of the ZmCIPK9 protein are obtained through research, and the ZmCIPK9 protein can be used for (1) cultivating transgenic plants; (2) improving plant varieties related to heat resistance; and (3) plant germplasm resource improvement. The discovery of the heat-resistant function of the ZmCIPK9 protein provides new gene targets and resources for cultivating heat-resistant plant varieties, and is of great significance to research on a heat-resistant molecular mechanism of corn.

The invention belongs to the technical field of aquaculture biological growth, and particularly relates to a gene for regulating fish growth, application and a regulating method. The gene for regulating the growth of the grass carp is a myogenesis inhibition gene mdfi of a myogenesis regulatory factor (MRFs) family; the base sequence of the gene is shown in SEQ ID NO: 1. The invention discloses a method for promoting proliferation of fish muscle cells. The expression level or protein activity of mdfi genes in grass carp muscle cells is inhibited. The inhibiting effect of the mdfi gene in the muscle growth of the grass carp is systematically clarified for the first time, the individual growth of the grass carp can be effectively promoted by establishing an mdfi gene knockout technology, and a new technical means and a new gene target are provided for the genetic breeding of the grass carp.

The application discloses CTL-S1 A gene and an application of miR-let-7 in regulation of reproductive development of drosophila bacterea CTL-S1 The gene is specifically highly expressed in ovaries of drosophila bacterea, and the expression of the gene is inhibited by RNA interference CTL-S1 The expression of the gene can reduce the hatching rate of offspring. Through analysis of an RNA immunoprecipitation double luciferase reporter system, it is found that miR-let-7 and CTL-S1 there is a target relationship. Overexpression of miR-let-7 can cause CTL-S1 the expression amount of the gene to be significantly reduced, and a phenotype similar to the expression of the gene being inhibited by RNA interference CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S is generated, and the hatching rate of offspring is reduced. The application provides a potential gene target for developing a genetic control strategy for destroying the reproduction of drosophila bacterea, provides a new idea for subsequent biological control of insects by using the sterile technique, and has a wide application prospect.

The present invention provides compositions and methods for novel digital PCR (dPCR) assay designs (droplet digital or other systems) for the detection and quantification of multiple Hepatitis B virus (HBV) gene targets. The compositions and methods can further include a non-extendible blocker oligonucleotide to reduce nonspecific inter-amplicon extension.

This invention relates to the field of biotechnology and discloses a method for regulating the angle of fruiting branches in upland cotton. GhPDF1 Genes and their applications, the aforementioned GhPDF1 The coding region sequence of the gene is shown in SEQ ID NO:1; GhPDF1 The gene ID is GH_D04G0386.1 ;A sort of GhPDF1 The application of genes in regulating the fruiting branch angle of upland cotton, either for regulating the fruiting branch angle of upland cotton or for breeding upland cotton varieties with a compact plant type, is described. GhPDF1 Genes regulate the fruiting branch angle of upland cotton by modulating their expression levels in upland cotton cells. This invention provides a precise genetic modification pathway, demonstrating the ability to inhibit [the growth of] upland cotton fruiting branches using VIGS technology. GhPDF1 Gene expression can significantly increase the angle between cotton fruiting branches, making the plant type looser, providing a clear gene target and effective technical means for using genetic engineering technology to cultivate new cotton varieties with compact plant type.

The invention relates to a multi-robot task allocation method and system based on multi-task optimization, and the method comprises the steps: constructing a mathematical model of multi-robot task allocation, and taking the minimization of the total path length and the minimization of the longest path as optimization targets; a multi-modal multi-target gene targeting differential evolution algorithm based on multi-task optimization is constructed, in the algorithm, a multi-robot task allocation problem which can not be returned to an original base station is set as a main task, and a multi-robot task which is returned to the original base station is set as an auxiliary task; based on the mathematical model and an improved differential evolution algorithm, task allocation of multiple robots is carried out; according to the improved differential evolution algorithm, information sharing of main tasks and auxiliary tasks of multiple robots is achieved through knowledge migration, a non-dominated solution is optimized in combination with a correction algorithm based on gene targeting, and an event triggering mechanism is adopted to allocate computing resources so as to control knowledge migration and auxiliary task optimization. Compared with the prior art, the method has the advantages of high global search capability, high optimization efficiency, high adaptability in a complex environment and the like.

The invention relates to application of a proteasome inhibitor in preparation of a medicine for treating tumors resistant to a tyrosine kinase inhibitor, and in the application, a plurality of strains of liver cancer cells with drug resistance to lenvatinib or regorafenib are constructed by utilizing a plurality of human liver cancer cell lines with different genetic backgrounds; a high-throughput CRISPR (clustered regularly interspaced short palindromic repeats) gene knockout screening technology and a patent medicine gene CRISPR library are utilized, and gene targets of which the gene knockout or inactivation can better kill drug-resistant cells are specifically identified from thousands of patent medicine genes. Wherein the function inactivation of a plurality of genes in the proteasome family shows more obvious cell killing activity in a plurality of drug-resistant cell models compared with non-drug-resistant cells. The invention discovers and verifies that the proteasome inhibitor has a specific killing effect on tyrosine kinase inhibitor drug-resistant liver cancer for the first time.

The invention relates to a gene for regulating and controlling boll shape and quality characters of cotton and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence of encoded protein of the gene is shown as SEQ ID NO.2. By constructing a gene editing vector and an overexpression vector and performing stable genetic transformation, the result shows that compared with wild type cotton Y668, a cotton Y668 mutant strain of which the gene is knocked out through a CRISPR / Cas9 technology has the advantages that the length-width ratio of a cotton boll is remarkably increased, the fiber length is remarkably increased, and under the background of Xinluzhong 75, the length of the cotton boll is remarkably increased. The length-width ratio of a cotton boll of an overexpression strain of the gene is obviously reduced, the fiber length is obviously shortened, the gene plays an important role in cotton boll shape and cotton fiber development, and important gene targets and germplasm resources are provided for cotton quality molecular breeding.

This invention provides a microfluidic chip for the multiplex differentiation and diagnosis of different avian influenza virus subtypes and its application. Specifically, it is a microfluidic chip based on TaqMan probes that can simultaneously and rapidly differentiate and diagnose 25 avian influenza subtypes, enabling high-throughput simultaneous detection of multiple gene targets. This invention utilizes high-throughput microfluidic chip technology to simplify the differential diagnosis procedure for avian influenza virus subtypes, resulting in more accurate and time-saving results. It is more suitable for rapid clinical diagnosis of avian influenza, providing technical support for rapid clinical detection, diagnosis, and prevention of avian influenza, and is of great significance for epidemic prevention and control and safeguarding public health security.

The invention discloses a soybean GmHRF-1 gene and application of protein coded by the soybean GmHRF-1 gene in improvement of soybean regeneration frequency and genetic transformation efficiency, and relates to the technical field of plant genetic engineering. The invention identifies and verifies that the GmHRF-1 gene in the soybean B3 transcription factor family has a brand new function of remarkably improving the regeneration capacity of somatic cells for the first time. By introducing the gene into soybean recipient cells and overexpressing the GmHRF-1, the regeneration frequency and genetic transformation efficiency of soybeans can be remarkably improved. The invention provides an efficient and universal endogenous gene for breaking through the technical bottleneck of genetic transformation of backbone germplasm which is difficult to regenerate of soybeans. The research result not only provides a new gene target for analyzing a molecular mechanism of soybean stem tip meristem regulation and regeneration, but also provides a technical support for constructing an efficient and stable soybean genetic transformation system.