56 results about "Plant virus" patented technology

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.

A cassette for the delivery of one or more mRNA is provided, the cassette comprising a 5' UTR, a Nodamura replicon, a ribosomal skipping polynucleotide, the one or more mRNA, a plant virus assembly origin polynucleotide ("OAS"), and a 3' UTR. In one aspect, the cassette further comprises a 5'mRNA cap. In a yet further aspect, the cassette does not comprise a poly A tail.

The invention belongs to the technical field of chemical prevention and control of agricultural diseases and insect pests, and provides an antiviral heterocyclic compound. The heterocyclic compound has a relatively good inhibition effect on plant viruses, has passivation, protection and treatment effects after being applied to plants, is superior to a commercial medicament virazole, has good biological activity and has a commercial prospect; the invention also provides a composition containing the heterocyclic compound. When the composition contains ningnanmycin, the two components have a remarkable synergistic effect in a certain proportion range, and the resistance risk of a single agent can be reduced. The invention provides a new medicament for preventing and treating plant viruses, and has important practical application value.

The utility model relates to the technical field of plant virus inoculation devices, in particular to a plant virus friction virus receiving device which comprises a liquid taking box and a friction virus receiving assembly. The liquid taking box is detachably connected with the friction poison receiving assembly; the friction poison receiving assembly comprises a bottom supporting box, the bottom supporting box is movably connected with a poison receiving box, the bottom supporting box is detachably connected with a liquid taking box, and the liquid taking box and the poison receiving box are oppositely arranged; a plurality of bristles are arranged on the inner wall of the poison receiving box, and the length of the bristles is equal to the depth of the liquid taking box; a movable bottom plate is arranged in the poison receiving box, the movable bottom plate can move towards or away from the liquid taking box, and the bristles movably penetrate through the movable bottom plate. According to the friction virus receiving device provided by the utility model, the friction stress of the blades can be strictly controlled, and the stress of the whole blades is uniform, so that the condition of insufficient friction or excessive force during inoculation is avoided. The device is suitable for laboratory novice, and can reduce the number of times of experiment groping and failure.

The invention relates to the technical field of nitrogen heterocyclic compounds, in particular to nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the specification, wherein X is selected from any one of-OCH3,-OCH2CH3 and-O (CH2) 2CH3. An antiviral activity test result shows that the azacyclo-alkaloid series compounds provided by the invention have antiviral activity, and the antiviral activity level of part of azacyclo-alkaloid reaches or even exceeds that of a positive control drug. Therefore, the azacyclo-alkaloid is expected to be applied to prevention and treatment of plant viruses.

The invention provides a coumoxystrobin compound composition for preventing and treating plant virus diseases and application of the coumoxystrobin compound composition, and relates to the technical field of pesticides, the coumoxystrobin compound composition comprises coumoxystrobin and an antiviral synergist, and the mass ratio of the coumoxystrobin to the antiviral synergist is 1: 20-20: 1; the antiviral synergist comprises at least one of ningnanmycin, amino-oligosaccharin or moroxydine hydrochloride, the antiviral effect of coumoxystrobin can be improved by compounding the antiviral synergist and coumoxystrobin, and the combination of the two has a remarkable synergistic effect; through the combination of different action mechanism components, the drug resistance risk of viruses to a single medicament is reduced, and the service life of the medicament is prolonged; the bactericidal composition has a good control effect on various plant virus diseases, and can be widely applied to various crops such as tobacco, tomatoes, cucumbers, peppers and the like. The technical problem that in the prior art, coumoxystrobin is poor in prevention and treatment effect on plant virus diseases is solved.

A method of treating cancer in a subject that includes administering in situ to the cancer of the subject a therapeutically effective amount of a plant virus or plant virus-like particle in combination with administration of an immune checkpoint therapy to the subject.

The invention provides qRT-PCR (quantitative real-time polymerase chain reaction) primers, a kit and a detection method for detecting tomato brown wrinkled fruit virus with high sensitivity, and belongs to the technical field of plant virus detection. The qRT-PCR primer for detecting the tomato brown wrinkled fruit virus, disclosed by the invention, is as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The invention also provides a qRT-PCR detection method of the tomato brown wrinkled fruit virus, the primer and the detection method provided by the invention have the characteristics of strong specificity and high sensitivity, the detection limit is 1.562 * 10 < 1 > copies / [mu] L, and the primer and the detection method have a better detection effect on a sample with low virus loading amount; and a technical support is provided for detecting the tomato brown wrinkled fruit virus and preventing and controlling the tomato brown wrinkled fruit virus.

This invention relates to plant genetic engineering, and more particularly to a variegated Phalaenopsis orchid and its preparation method, the preparation method comprising the following steps: (1) according to the target gene with the base sequence shown in SEQ ID No. 1 PePDS (1) Synthesize specific nucleotide fragments; (2) Construct specific nucleotide fragments into a plant virus vector; (3) Introduce the recombinant plant virus vector obtained in step (2) into Phalaenopsis orchid plant material; (4) Screen to obtain Phalaenopsis orchids with silenced target genes. This invention achieves rapid, efficient, and non-transgenic leaf color variegation creation in commercial Phalaenopsis orchid varieties for the first time through VIGS technology; it not only breaks through the technical bottleneck of difficult and long cycle of genetic manipulation of orchid plants, significantly shortening the trait verification cycle, but also induces diverse and highly ornamental variegated leaf phenotypes such as albino, yellow, or purple in different varieties, and has the advantages of simple operation, low cost, and industrialization feasibility.

The invention relates to the technical field of plant growth regulation, in particular to application of berberine based on ACAA targets in resisting plant viruses and promoting plant growth. The invention reveals that berberine plays a role by specifically targeting acetyl coenzyme A acyltransferase and up-regulating the expression of the acetyl coenzyme A acyltransferase for the first time. The clear compound-target-pathway mechanism overcomes the defect that the existing plant source product mechanism is unknown. As the target spot is clear, the application dosage can be accurately controlled, so that the stability and predictability of field antiviral and growth promoting effects are ensured, and a foundation is laid for large-scale agricultural application. An ACAA target is activated by berberine, and an antiviral defense mechanism and an energy metabolism growth pathway of a plant can be synchronously started, so that the plant growth is effectively promoted while virus diseases are prevented and treated. The disease resistance-growth negative correlation problem existing in agricultural production for a long time is solved, and the dual requirements of modern agriculture for precise prevention and control and yield increase and quality improvement are met.

The invention discloses chloroindole hydrazide-loaded nanogel and a preparation method and application thereof, and relates to the technical field of pesticides, the chloroindole hydrazide-loaded nanogel is successfully prepared, CHI can be stably loaded, slow release is realized, and the chloroindole hydrazide-loaded nanogel has excellent leaf adhesiveness, can resist rain wash and avoid loss of pesticide effect, and can be used as a pesticide for preventing and treating diseases. The application pain point of CHI and the defects of a traditional nano-carrier are overcome; the nano-scale characteristic assists plant cell endocytosis and uptake, breaks through the transport limitation of cell membranes, and gives full play to the high induced resistance activity of CHI. High-speed centrifugal purification is not needed in the preparation process, the process is simplified, the yield is increased, large-scale production is adapted, and meanwhile energy consumption and cost are reduced. Compared with a traditional nano-carrier, the nano-gel does not need expensive precursors or complex modification, is good in dispersity and free of the agglomeration problem, preparation is low in energy consumption and pollution, the comprehensive performance is more suitable for practical application of pesticide carriers, and a solution with practicability and economical efficiency is provided for efficient prevention and treatment of plant virus diseases.

The invention discloses bacillus subtilis subsp.deserticola, nano-selenium synthesis application of the bacillus subtilis subsp.deserticola and application of the bacillus subtilis subsp.deserticola to prevention and treatment of plant virus diseases, and belongs to the technical field of microorganisms. The bacillus subtilis subsp. Deserticola KBD-4 has better selenium tolerance and can reduce selenium salt into nano-selenium, and meanwhile, a bacterial solution and a nano-selenium active bacterial solution which are generated after the strain is fermented have a better inhibition effect on plant viruses such as TMV, CMV, PVY and TSWV, so that the plant virus diseases can be better prevented and treated. Besides, a biological source nano-selenium potassium fertilizer can be prepared by adding a high-potassium trace element additive into the nano-selenium active bacterial liquid, after root irrigation, the disease-resistant and growth-promoting effects on the tomatoes are better, the tomato yield is increased, the nutritional quality of the tomatoes is further improved, selenium-rich agricultural products meeting the national standard are produced, and the method is worthy of popularization and application. The method has good practical application value in production.

The invention relates to an indole diazepine derivative as well as a preparation method and application thereof, the structural formula is as shown in formula I. Anti-plant virus activity test on the indole diazepine derivative proves that the indole diazepine derivative has good antiviral activity on pepper mild mottle virus. The compound can be used for preparing a pepper mild mottle virus disease prevention and control medicament, and the preparation method is simple and low in production cost.

The invention discloses a pH-responsive isoGPBG hydrogel and an application of the pH-responsive isoGPBG hydrogel in miRNA delivery, and belongs to the technical field of biopesticides. The pH-responsive isoGPBG hydrogel disclosed by the invention is formed by deprotonating isoguanosine and guanosine under an alkaline condition and cross-linking the deprotonated isoguanosine and guanosine with a boric acid group of PBA to form a pH-sensitive phenylboronic acid ester bond. The hydrogel delivery system can break through plant miRNA transmembrane transport obstacles, and efficient delivery and controllable release of antiviral nucleic acid drugs are achieved. When the hydrogel delivery system is used for delivering miRNA, in-vitro degradation of miRNA can be prevented, and the hydrogel delivery system has an important application prospect in prevention and treatment of plant viral diseases and bacterial diseases.

The present invention provides intravenous pharmaceutical compositions comprising engineered plant virus-based nanoparticles for the treatment or prevention of autoimmune conditions. The nanoparticles display immunodominant peptides that induce immune tolerance in a subject when administered intravenously. The invention further provides methods of manufacturing virus-based nanoparticles, including production in plant expression systems and optional viral inactivation, to render them suitable for therapeutic use in mammals. Also provided are methods of intravenous administration of the pharmaceutical compositions to a subject in need thereof for treating or preventing autoimmune conditions.

The application provides a kind of 3, 4-dichloroisothiazole benzamide derivatives and preparation method and purposes thereof, specifically relates to a kind of derivatives, and the chemical structure general formula is seen formula I: the application discloses the general formula of the structure of the above compound, synthesis method and as insecticide, acaricide, fungicide, anti-plant virus agent, plant activator purposes, it is combined with agricultural acceptable adjuvant or synergist and with commercial insecticide, acaricide, fungicide, anti-plant virus agent, plant immune activator in the prevention and treatment of agricultural, forestry, horticultural plant pest, mite damage, disease, viral disease purposes and preparation method.

The present application relates to the technical field of aquatic plant virus separation, and discloses a method for separating aquatic plant viruses and application thereof, wherein the method comprises mixing amino-functionalized carbon nanomaterials and aquatic bodies containing plant viruses. The amino-functionalized carbon nanomaterials used in the method can be combined with the plant viruses in the aquatic bodies, thereby improving the adsorption capacity of the amino-functionalized carbon nanomaterials for the plant viruses. The method is simple, efficient and low in cost, and does not produce toxic substances, thereby avoiding secondary pollution possibly caused by chemical disinfection, providing an economic and sustainable solution to the problem of plant virus pollution, and showing a broad application prospect in the field of aquatic plant virus removal.

The present application relates to the field of agricultural technology, and particularly relates to an amine group-containing alstoniascholaris alkaloid derivative, a preparation method and application thereof. The amine group-containing alstoniascholaris alkaloid derivative comprises a compound as shown in formula (I): wherein R comprises at least one of C3-C12 linear alkyl, C3-C12 branched alkyl, C3-C6 cycloalkyl, C3-C6 cycloalkylmethyl, C2-C6 aminoalkyl, tetrahydropyrrole group, piperidine group, piperidinylmethyl, N-methylpiperidine group and N-methylpiperidinylmethyl. The amine group-containing alstoniascholaris alkaloid derivative has good plant virus control and good fungicidal activity.

The invention provides a cyclopentene chromene-cinnamate compound as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis. According to the invention, mangiferin is taken as a guide, and a cyclopentenochromene structure is designed and synthesized from cyclopentenopyrane and a chromene skeleton by using molecular hybridization and skeleton transition strategies. According to the cyclopentenochromene-cinnamate derivative containing the cinnamic acid active fragment, the cinnamic acid fragment with pesticide activity is grafted to the 8-site of cyclopentenochromene, the cyclopentenochromene-cinnamate derivative containing the cinnamic acid active fragment is synthesized, and the obtained compound has good insecticidal activity, bactericidal activity and plant virus resisting activity.

The present application provides a kind of containing isochromene pyridone derivatives and its preparation method and purposes, specifically related to a kind of derivatives, its chemical structure general formula see formula I: The present application discloses the general formula of the structure of the above compound, synthesis method and as insecticide, fungicide, anti-plant virus agent purposes, it is combined with agricultural acceptable adjuvant or synergist and with commercial insecticide, fungicide, anti-plant virus agent, miticide in the prevention and treatment of agricultural, forestry, horticultural plant pest, disease, viral disease purposes and preparation method.

The invention relates to the technical field of plant virus detection, in particular to a pepper vein flavivirus detection method and system based on RT-RAA-CRISPR / Cas12a and application of the pepper vein flavivirus detection method and system. The invention discloses a pepper vein flavivirus detection method based on RT-RAA-CRISPR / Cas12a. The pepper vein flavivirus detection method comprises the following steps: S1, extracting virus RNA (Ribonucleic Acid) from a plant leaf to be detected; s2, adding RNA (Ribonucleic Acid) of a sample to be detected into the RT-RAA amplification system, and carrying out isothermal amplification at 37-42 DEG C for 10-30 minutes to obtain an RT-RAA amplification product; s3, adding the RT-RAA amplification product into a CRISPR / Cas12a (clustered regularly interspaced short palindromic repeats / CRISPR associated 12a) detection system, and reacting for 20 to 30 minutes under the condition of 37 DEG C; s4, detecting fluorescence change in real time, and judging a result through a fluorescence signal; if a fluorescence signal is detected, it is indicated that the pepper vein flavivirus exists in the RNA of the to-be-detected sample, efficient amplification is achieved through a recombinase-single-chain binding protein-primer compound, and high sensitivity and specificity of detection are ensured through accurate nucleic acid recognition and signal amplification functions; the method is especially suitable for rapid screening and diagnosis of plant viruses in limited resource environments such as fields.

The invention discloses a cultivation method for efficient in-vitro rapid propagation of sweet potato virus-free seedlings, and belongs to the technical field of plant breeding. The method comprises the following steps: by taking new buds germinated from excellent plant tubers of a bred sweet potato variety as a material source, performing stem efficient adventitious bud induction and sterile system establishment, stem tip stripping and bud induction, adventitious bud proliferation and elongation synchronous culture, regenerated plant detoxification effect detection, ex-vitro adventitious root induction and domestication transplanting synchronous culture and the like. And finally, efficient propagation of the detoxified sweet potato seedlings is realized. The method provides key technical support for detoxification of sweet potato excellent strain seedlings and industrialized production of detoxified seedlings, and can meet the annual requirements of industrialized development on high-quality seedlings.

This invention discloses primers, probes, a kit, and a method for detecting TobRFV brown wrinkled fruit virus based on MIRA technology, belonging to the field of plant virus detection technology. The primers and probes include an upstream primer ToBRFV-ORF1-3F3, a downstream primer ToBRFV-ORF1-3R6, and a probe ToBRFV-ORF1-P3. The nucleotide sequence of the upstream primer ToBRFV-ORF1-3F3 is shown in SEQ ID NO:15, the nucleotide sequence of the downstream primer ToBRFV-ORF1-3R6 is shown in SEQ ID NO:21, and the nucleotide sequence of the probe ToBRFV-ORF1-P3 is shown in SEQ ID NO:2. The kit of this invention can achieve highly sensitive and specific rapid amplification of ToBRFV nucleic acid under isothermal conditions, and is suitable for rapid on-site detection in non-laboratory environments such as fields and greenhouses.

This invention discloses a one-step RT-PCR method for detecting five viruses in *Calamus sylvestris*, belonging to the field of plant virus molecular detection technology. Specifically, it provides a primer set for the simultaneous detection of multiple viruses in *Calamus sylvestris*, including five pairs of virus-specific primers for the specific detection of PVBV, CaYMAV, CaYMV, CaYSV, and SCMV, and one pair of *Calamus sylvestris* internal reference gene primers, the sequences of which are shown in SEQ ID NO:1-12. RNA is extracted from *Calamus sylvestris* samples, a one-step RT-PCR reaction system is prepared, amplification and electrophoresis are performed, the detection validity is verified by the internal reference gene band, and the type of virus infection is determined by the virus-specific bands. This invention can achieve simultaneous detection of five viruses and has the advantages of simple operation, short detection cycle, high sensitivity, and strong specificity, making it suitable for rapid screening of large batches of *Calamus sylvestris* samples.

The utility model relates to the technical field of plant virus detection, in particular to a plant virus sample extracting device which comprises a sampling box, the sampling box is provided with a material channel, a feeding block is arranged in the material channel and matched with the material channel, a feeding screw is arranged on the right side of the feeding block, and the left end of the feeding screw is rotationally connected with the feeding block. The right end is in threaded connection with the sampling box; the material channel is sequentially divided into a feeding part, a cutting part and a pressing part from right to left, and a feeding port is formed in the top of the feeding part; a cutter is arranged on the upper portion of the cutting portion, and a cutter collecting groove is formed in the top in the cutting portion and corresponds to the cutter. A plurality of through holes are formed in the bottom wall of the pressing part, a containing groove is formed in the top wall of the pressing part, an extrusion block is movably embedded in the containing groove, an extrusion rod is fixed to the top of the extrusion block, and the extrusion rod slides upwards to penetrate through the top wall of the pressing part; a slag outlet is formed in the left end of the material channel. According to the utility model, cutting, squeezing and filtering of samples can be completed in a closed environment, and the probability that the samples are polluted is reduced.

The application discloses application of a polysaccharide of Ossyra edulis in preparation of an anti-plant virus agent. The polysaccharide is obtained by alkali extraction and alcohol precipitation of dry sclerotia of Ossyra edulis. The application finds that the polysaccharide of Ossyra edulis shows significant anti-virus activity on various plant pathogenic viruses in a pot experiment, and its preparation also shows good activity in a field efficacy test, and is significantly better than a positive control agent. Compared with the prior art, the application develops an anti-virus agent pesticide from a biological source of natural substances, has good environmental compatibility, safety, high efficiency, green environmental protection and the like, and has a wide application prospect.

The present invention provides a plant RNA virus detection method that is highly efficient in detection while achieving labor savings and lower costs compared to conventional techniques. [Solution] A plant RNA virus detection method comprising a step of heat-treating a mixture obtained by grinding a plant, the mixture being mixed with a primer solution, wherein the heat-treating step heats the mixture at 60°C to 98°C for 4 to 6 minutes. Alternatively, the plant RNA virus detection method is one in which the plant grinding mixture is obtained by grinding plant tissue together with a solution containing at least one selected from the group consisting of a buffer solution and RNase-free water.

This invention belongs to the field of pesticides and provides a composition containing toxic fluoride for the control of plant viral diseases. The composition uses toxic fluoride and (1S,3S)-N'-(4-chlorophenylmethylene)-1-methyl-2,3,4,9-tetrahydropyrido[3,4-b]indole-3-carboxylhydrazine as active ingredients, with the active ingredients accounting for 20%-45% of the total composition by weight. This composition can be used to control viral diseases in various plants, such as cucumbers, tobacco, celery, peppers, and potatoes. It can also improve crop resistance to drought and high temperatures, regulate crop growth, promote flowering, and increase fruit yield and quality.

The application discloses a cucumber aphid-susceptible regulation gene CsAGO5a and application thereof, including that a nucleotide sequence thereof is shown as SEQ ID NO. 1. An expression protein of the cucumber aphid-susceptible regulation gene CsAGO5a has an amino acid sequence shown as SEQ ID NO. 2. A plant virus carrier pV190 containing the cucumber aphid-susceptible regulation gene CsAGO5a. The carrier silences the CsAGO5a gene and has low expression in plants. The introduced carrier is pV190, and linearization treatment is carried out on the pV190 after enzyme cutting using BamH I to obtain a recombined carrier. The application takes a cucumber main cultivar "Superina" as material, silences the CsAGO5a gene through a VIGS technology, and carries out a non-selective colonization experiment and the like, proves that the application is related to plant aphid resistance, has important application value in the fields of cucumber breeding such as enhancing plant resistance, improving aphid resistance screening methods and creating germplasm resources and the like.