182 results about "Plant virus" patented technology

The invention discloses a target segment for preventing and treating tomato brown fruit wrinkling virus in tobacco, double-stranded RNA (Ribonucleic Acid) and application of the target segment and the double-stranded RNA. Wherein the nucleotide sequence of the target segment is as shown in SEQ ID NO.3, or a sequence which is as shown in SEQ ID NO.3, has at least 90% of homology and has coded amino acid as shown in SEQ ID NO.4 is substituted. The invention also discloses a double-stranded RNA of a targeted interference target section, and the double-stranded RNA can significantly inhibit the proliferation of the tomato brown crinkled fruit virus in tobacco by injecting or smearing tobacco leaves, so that the double-stranded RNA can be effectively applied to prevention and control of tobacco diseases caused by the tomato brown crinkled fruit virus. The method for preventing and controlling the tomato brown crinkled fruit virus in the tobacco by using the double-stranded RNA is safe, harmless and efficient, and provides a new path for preventing and controlling plant virus diseases by using RNAi (Ribonucleic Acid Interference).

The invention belongs to the technical field of plant virus detection, and provides a multiplex RT-PCR detection method for areca-nut related viruses. The primer group for multiple RT-PCR detection of areca-related viruses (areca yellow virus APV1, areca ringspot virus ANRSV and areca shuttle spot virus ANSSV) comprises a first primer group for detecting the APV1, a second primer group for detecting the ANRSV and a third primer group for detecting the ANSSV. The invention also provides a detection kit, which comprises a PCR reaction solution, and the PCR reaction solution comprises the primer group. The invention also provides application of the kit in simultaneous detection of areca-related viruses APV1, ANRSV and ANSSV. The primer group and the kit disclosed by the invention can specifically detect three areca-nut related viruses in a PCR system, can accurately identify any combination infection of the three viruses, and have the characteristics of high efficiency, economy, simplicity and convenience.

The current invention relates to compounds of the formula (I) wherein the substituents are as defined in claim 1, to processes and methods for preparing compounds of formula (I), to agrochemical compositions comprising compounds of formula (I) as defined in claim 1, to preparation of these compositions and to the use of the compounds or compositions in agriculture or horticulture for combating, preventing or controlling infestation of plants, harvested food crops, seeds or non-living materials by phytopathogenic microorganisms, in particular fungi.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses an MQDs-PEI-coated miR43-CMCS / TA composite nanomaterial for preventing and treating plant viruses as well as a preparation method and application of the MQDs-PEI-coated miR43-CMCS / TA composite nanomaterial, and belongs to the technical field of genetic engineering. The composite nano material is prepared from Mxene-QDs, polyetherimide, carboxymethyl chitosan, tannic acid and miR43 nano microspheres, the nano composite material is small in particle size and high in drug loading capacity, has an excellent protection effect and pH release capacity on miR43, can deliver miR43 into a plant body in a spraying mode and the like, and can be used for preparing the miR43 in the plant body by specifically shearing NbIAA29 in the plant. Therefore, replication and infection of the PVY virus and the TMV virus are inhibited, and important significance is achieved on prevention and treatment of the PVY virus and the TMV virus.

The invention discloses a tobacco antiviral protein gene NbSRC2 and application of the tobacco antiviral protein gene NbSRC2 in inhibiting tobacco infected by plant viruses, and belongs to the technical field of biology and molecular biology. The amino acid sequence of the tobacco antiviral protein gene NbSRC2 is as shown in SEQ ID NO: 2, and the nucleotide sequence is as shown in SEQ ID NO: 1. The NbSRC2 is silenced through a VIGS technology, and the virus coat protein CP mRNA, the virus coat protein CP and the virus infection symptom difference show that the silent NbSRC2 up-regulates the sensitivity of nicotiana benthamiana to TMV infection and promotes virus infection. The TMV infection is inhibited by overexpression of the NbSRC2. The NbSRC2 can inhibit the infection of plant viruses, is a potential antiviral gene on tobacco, can be used as a potential antiviral protein, and further enriches the scope of antiviral genes.

The invention belongs to the technical field of plant cultivation, and relates to a cultivation method for preventing and controlling plant viral diseases. The invention provides a cultivation method for preventing and controlling plant viral diseases. The cultivation method comprises the following steps: doping a soil microorganism conditioning additive when furrowing and applying a base fertilizer in a field, and doping the soil microorganism conditioning additive when dressing a fertilizer, the soil microorganism conditioning additive is composed of sodium alginate, a crushed material of an underground part of Khaxican eggplant and a crushed material of an underground part of rubus alceolatus. The cultivation method provided by the invention can effectively solve the technical problem that viral diseases of various plants are difficult to control, and the yield of crops such as tobaccos, tomatoes and peppers is increased.

The invention provides a plant viral vector comprising a heterologous nucleotide sequence that encodes, or corresponds to the RNA sequence of a functional minimal miRNA precursor, wherein said RNA sequence consists of S1 that is of a length of 56-400 ribonucleotides and that comprises regions R1, R2, R3, R4 and R5 directly linked to each other as indicated in formula (i): (i) 5'-R1-R2-R3-R4-R5-3', wherein: - R1 consists of the RNA sequence of a strand of a basal stem (BS) structure, - R2 consists of the RNA sequence of a miRNA, - R3 comprises an RNA sequence that has a length of at least 8 nucleotides, - R4 consists of an RNA sequence that base pairs with the RNA sequence of R2 to form a miRNA / miRNA* duplex, - R5 consists of the RNA sequence complementary to R1 in the BS comprising R1, and wherein the RNA sequence S1 forms a stem-loop structure where the stem comprises regions R1, R2, R4 and R5 wherein region R1 base pairs with region R5 and region R2 base pairs with region R4, and the loop comprises a sequence of at least 4 contiguous nucleotides of region R3. The invention also provides a virus encoded by or comprising the viral vector, a plasmid comprising or encoding the viral vector, a bacterium comprising the plasmid, a plant comprising the viral vector, the virus or the bacterium, a plant extract from the plant, and a method for obtaining the plant and a method for obtaining the extract.

The invention discloses a primer, a kit and a detection method for detecting tomato brown wrinkled fruit virus based on a digital PCR technology, and relates to the technical field of plant virus biological detection.The primer comprises ToBRFV-CqF and ToBRFV-CqR, the nucleotide sequence of the ToBRFV-CqF is shown as SEQ ID NO: 1, and the nucleotide sequence of the ToBRFV-CqR is shown as SEQ ID NO: 2. The method disclosed by the invention has extremely high sensitivity, specificity and repeatability, can be used for accurately detecting a sample with extremely low virus loading quantity, and can be directly used for quantitatively monitoring the tomato brown wrinkled fruit virus in the field.

The invention relates to the technical field of plant disease control, in particular to application of a quinazoline carboxylic acid compound in plant virus resistance. The invention discovers that 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid (DHCA) has the effect of resisting plant viruses for the first time. In the specific embodiment of the invention, the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid is used for preventing and treating the cucumber virus disease and the pepper virus disease, and the result shows that the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid and the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid are used for preventing and treating the cucumber virus disease and the pepper virus disease. And the composition has a good control effect by combining the 2, 2-a] quinazoline-3a (1H)-carboxylic acid with wuyiencin.

The invention discloses bacillus subtilis subsp.deserticola, nano-selenium synthesis application of the bacillus subtilis subsp.deserticola and application of the bacillus subtilis subsp.deserticola to prevention and treatment of plant virus diseases, and belongs to the technical field of microorganisms. The bacillus subtilis subsp. Deserticola KBD-4 has better selenium tolerance and can reduce selenium salt into nano-selenium, and meanwhile, a bacterial solution and a nano-selenium active bacterial solution which are generated after the strain is fermented have a better inhibition effect on plant viruses such as TMV, CMV, PVY and TSWV, so that the plant virus diseases can be better prevented and treated. Besides, a biological source nano-selenium potassium fertilizer can be prepared by adding a high-potassium trace element additive into the nano-selenium active bacterial liquid, after root irrigation, the disease-resistant and growth-promoting effects on the tomatoes are better, the tomato yield is increased, the nutritional quality of the tomatoes is further improved, selenium-rich agricultural products meeting the national standard are produced, and the method is worthy of popularization and application. The method has good practical application value in production.

The invention discloses a natural tobacco extract and application thereof in prevention and treatment of plant viruses, and belongs to the technical field of agricultural drugs and biology. A natural extract is obtained from tobacco leaves slightly infected with viruses, and experiments show that when the tobacco natural extract is sprayed on the plant leaves infected with the viruses, the immune defense capability of plants can be induced, propagation and replication of the viruses can be effectively inhibited, the disease resistance of the plants is enhanced, and the effect of preventing and treating the viruses is achieved. Therefore, the purpose of improving crop yield and quality is achieved. The natural tobacco extract can be used as an effective component of a plant antiviral agent, and a new direction is provided for research and development of the plant antiviral agent. The method for preventing and treating plant viruses by externally spraying the natural tobacco extract has the advantages of being environmentally friendly, non-toxic and harmless, is an ideal plant virus prevention and treatment technology, is simple and efficient, can keep high stability and practicability in field application, and meets the current requirements for ecological safety and sustainable development.

Provided are compositions, methods and uses relating to conjugate comprising a liposome encapsulating one or cancer antigen(s) that is conjugated to a plant virus nanoparticle that is useful to treat cancer.

The invention relates to a primer for visual detection of ToBRFV and application thereof, and belongs to the technical field of plant virus detection, the primer comprises an upstream outer primer ToBRFV-F3 with a nucleotide sequence as shown in SEQ ID No.2, a downstream outer primer ToBRFV-B3 with a nucleotide sequence as shown in SEQ ID No.3, an upstream inner primer ToBRFV-FIP with a nucleotide sequence as shown in SEQ ID No.4, and a downstream inner primer ToBRFV-BIP with a nucleotide sequence as shown in SEQ ID No.5. In addition, the invention also comprises the application of the primer in the visual detection of the ToBRFV based on LAMP-CRISPR / Cas12a. The method is sensitive and accurate in ToBRFV detection, and has the advantages of easiness in operation, rapidness in detection, high specificity, high sensitivity, visualization and the like.

The invention relates to the technical field of plant virus detection, in particular to a system and a method for detecting areca yellowing related viruses based on an RPA-LFD technology. The primer group for detecting the areca yellows virus APV1 is characterized by comprising a specific primer pair, the nucleotide sequence of an upstream primer in the specific primer pair is as shown in SEQ ID No.1 in a sequence table, and the nucleotide sequence of a downstream primer is as shown in SEQ ID No.2 in the sequence table; the device further comprises a probe. The probe is labeled and modified by biotin or FAM fluorophore; the nucleotide sequence of the modified probe is as shown in SEQ ID No.3 in the sequence table. The kit has the advantages that the kit has higher specificity and lower detection limit in the aspect of detection, and has no cross reaction with other viruses such as areca necrotic ringspot virus; the primer probe combination is suitable for RPA-LFD detection, and the time required by an amplification reaction is short; the reaction result is shown on the test strip and can be observed by naked eyes.

Provided are a cryptolepine salt derivative, and a preparation method therefor and the use thereof. The cryptolepine salt derivative comprises a compound as represented by formula (I). By means of introducing different substituents into the cryptolepine salt derivative, a biological activity of the compound is improved by means of the regulation effects of the substituents on the electrical property and the solubility of the cryptolepine salt derivative, so that the obtained cryptolepine salt derivative has a good prevention and control effect on plant viruses and a bactericidal activity.

The invention belongs to the technical field of biology, and particularly relates to an OsATG8b gene and application of an encoding protein of the OsATG8b gene to regulation and control of plant virus resistance of rice. The OsATG8b gene is derived from rice, the nucleotide sequence of the OsATG8b gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the OsATG8b gene is shown as SEQ ID NO.2. By cloning the OsATG8b gene and constructing a transgenic vector, OsATG8b gene overexpressed rice and OsATG8b gene knockout rice are obtained, the resistance of the OsATG8b gene overexpressed rice and OsATG8b gene knockout rice to watery straw dwarf virus and rice serrated leaf dwarf virus is measured, and the result shows that compared with wild type rice and OsATG8b gene knockout rice, the OsATG8b gene overexpressed rice and OsATG8b gene knockout rice have the advantages that the OsATG8b gene overexpressed rice and OsATG8b gene knockout rice has higher rice resistance to watery straw dwarf virus and rice serrated leaf dwarf virus; the OsATG8b gene overexpressed rice has higher resistance to rice straw dwarf virus and rice serrated leaf dwarf virus, which indicates that the OsATG8b gene plays an important regulation and control role in rice plant virus resistance.

A fungicidal composition comprising a mixture of components (A) and (B), wherein components (A) and (B) are as defined in claim 1, and use of the compositions in agriculture or horticulture for controlling or preventing infestation of plants by phytopathogenic microorganisms, preferably fungi.

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.

This disclosure provides compositions and combinations for treating cancer. The compositions or combinations comprise a plant virus and a natural killer (NK) cell agonist.

A method of treating cancer in a subject that includes administering in situ to the cancer of the subject a therapeutically effective amount of a plant virus or plant virus-like particle in combination with administration of an immune checkpoint therapy to the subject.

A cassette for the delivery of one or more mRNA is provided, the cassette comprising a 5' UTR, a Nodamura replicon, a ribosomal skipping polynucleotide, the one or more mRNA, a plant virus assembly origin polynucleotide ("OAS"), and a 3' UTR. In one aspect, the cassette further comprises a 5'mRNA cap. In a yet further aspect, the cassette does not comprise a poly A tail.

The invention discloses a preparation process and application of celandine alkaloid for preventing and treating plant diseases, on the basis of a single factor test, polynomial fitting regression is carried out by adopting a Box-Behnken response surface method and taking oxalic acid concentration, liquid-to-solid ratio, ethanol concentration, ultrasonic time and extraction temperature as influence factors to obtain a regression equation, and the regression equation is used as a regression equation to obtain the celandine alkaloid for preventing and treating the plant diseases. The optimal total alkaloid extraction method is as follows: the oxalic acid concentration is 0.6%, the liquid-material ratio is 17: 1, the ethanol concentration is 70%, the ultrasonic time is 47min, the extraction temperature is 52 DEG C, and the extraction yield is 9.20 mg / mL. According to the method, strong acid is replaced with weak acid in the extraction process of the celandine total alkaloids, corrosion to equipment in the production process is reduced, production safety is improved, environmental safety is improved, the extraction process is optimized through a response surface method, and the product yield is increased. An indoor biological assay test finds that the celandine total alkaloid has good bacteriostatic activity and plant virus resisting activity, and has the potential of being further developed into a botanical fungicide and an antiviral agent.

The invention belongs to the technical field of chemical prevention and control of agricultural diseases and insect pests, and provides an antiviral heterocyclic compound. The heterocyclic compound has a relatively good inhibition effect on plant viruses, has passivation, protection and treatment effects after being applied to plants, is superior to a commercial medicament virazole, has good biological activity and has a commercial prospect; the invention also provides a composition containing the heterocyclic compound. When the composition contains ningnanmycin, the two components have a remarkable synergistic effect in a certain proportion range, and the resistance risk of a single agent can be reduced. The invention provides a new medicament for preventing and treating plant viruses, and has important practical application value.

The utility model relates to the technical field of plant virus inoculation devices, in particular to a plant virus friction virus receiving device which comprises a liquid taking box and a friction virus receiving assembly. The liquid taking box is detachably connected with the friction poison receiving assembly; the friction poison receiving assembly comprises a bottom supporting box, the bottom supporting box is movably connected with a poison receiving box, the bottom supporting box is detachably connected with a liquid taking box, and the liquid taking box and the poison receiving box are oppositely arranged; a plurality of bristles are arranged on the inner wall of the poison receiving box, and the length of the bristles is equal to the depth of the liquid taking box; a movable bottom plate is arranged in the poison receiving box, the movable bottom plate can move towards or away from the liquid taking box, and the bristles movably penetrate through the movable bottom plate. According to the friction virus receiving device provided by the utility model, the friction stress of the blades can be strictly controlled, and the stress of the whole blades is uniform, so that the condition of insufficient friction or excessive force during inoculation is avoided. The device is suitable for laboratory novice, and can reduce the number of times of experiment groping and failure.

The invention relates to the technical field of nitrogen heterocyclic compounds, in particular to nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the specification, wherein X is selected from any one of-OCH3,-OCH2CH3 and-O (CH2) 2CH3. An antiviral activity test result shows that the azacyclo-alkaloid series compounds provided by the invention have antiviral activity, and the antiviral activity level of part of azacyclo-alkaloid reaches or even exceeds that of a positive control drug. Therefore, the azacyclo-alkaloid is expected to be applied to prevention and treatment of plant viruses.

The application relates to the technical field of nitrogen heterocyclic compounds, in particular to a nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the description, X is selected from any one of -OCH3, -OCH2CH3 and -O(CH2)2CH3. The anti-virus activity test result shows that the nitrogen heterocyclic alkaloid series compounds provided by the application all have anti-virus activity, wherein the anti-virus activity level of part of the nitrogen heterocyclic alkaloids reaches or even exceeds that of a positive control drug. Therefore, the nitrogen heterocyclic alkaloid can be expected to be applied to the prevention and treatment of plant viruses.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for transferring an exogenous nucleic acid fragment into a plant by using a plant virus. The technical problem to be solved by the invention is to provide a new method which can be applied to transferring an exogenous fragment into a plant. According to the technical scheme, the method for transferring the exogenous nucleic acid fragment into the plant by using the plant virus comprises the following steps: fusing the exogenous nucleic acid fragment into a 3'non-coding RNA region of TVMVwt to obtain a fused fragment, constructing the fused fragment onto an infectious clone skeleton vector, and transforming the plant. According to the invention, an exogenous coding sequence is inserted into a 3'non-coding RNA region of TVMVwt to construct an infectious cloning vector, the infectious cloning vector is transferred into a plant, and expression of the exogenous coding sequence is realized.

The present invention relates to a highly effective, broad-spectrum anti-plant viral disease pharmaceutical composition and its application. The active ingredients of the anti-plant viral disease pharmaceutical composition include chlorin iron and lentinan; wherein the weight ratio of chlorin iron to lentinan is 1:6000 to 4:1. When a certain concentration of the chlorin iron and lentinan pharmaceutical composition is applied to plants, the two active ingredients exert a synergistic effect, alleviating symptoms of diseased plants, reducing disease indexes, and reducing viral accumulation in the plants. The plant anti-viral disease pharmaceutical composition is a biological agent that is environmentally friendly, safe, and environmentally friendly. It has important application prospects in combating plant viral diseases, preventing and controlling plant diseases caused by plant viruses, and improving plant quality.

The invention provides a coumoxystrobin compound composition for preventing and treating plant virus diseases and application of the coumoxystrobin compound composition, and relates to the technical field of pesticides, the coumoxystrobin compound composition comprises coumoxystrobin and an antiviral synergist, and the mass ratio of the coumoxystrobin to the antiviral synergist is 1: 20-20: 1; the antiviral synergist comprises at least one of ningnanmycin, amino-oligosaccharin or moroxydine hydrochloride, the antiviral effect of coumoxystrobin can be improved by compounding the antiviral synergist and coumoxystrobin, and the combination of the two has a remarkable synergistic effect; through the combination of different action mechanism components, the drug resistance risk of viruses to a single medicament is reduced, and the service life of the medicament is prolonged; the bactericidal composition has a good control effect on various plant virus diseases, and can be widely applied to various crops such as tobacco, tomatoes, cucumbers, peppers and the like. The technical problem that in the prior art, coumoxystrobin is poor in prevention and treatment effect on plant virus diseases is solved.