97 results about "Plant virus" patented technology

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention belongs to the technical field of plant cultivation, and relates to a cultivation method for preventing and controlling plant viral diseases. The invention provides a cultivation method for preventing and controlling plant viral diseases. The cultivation method comprises the following steps: doping a soil microorganism conditioning additive when furrowing and applying a base fertilizer in a field, and doping the soil microorganism conditioning additive when dressing a fertilizer, the soil microorganism conditioning additive is composed of sodium alginate, a crushed material of an underground part of Khaxican eggplant and a crushed material of an underground part of rubus alceolatus. The cultivation method provided by the invention can effectively solve the technical problem that viral diseases of various plants are difficult to control, and the yield of crops such as tobaccos, tomatoes and peppers is increased.

The invention relates to the technical field of plant disease control, in particular to application of a quinazoline carboxylic acid compound in plant virus resistance. The invention discovers that 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid (DHCA) has the effect of resisting plant viruses for the first time. In the specific embodiment of the invention, the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid is used for preventing and treating the cucumber virus disease and the pepper virus disease, and the result shows that the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid and the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid are used for preventing and treating the cucumber virus disease and the pepper virus disease. And the composition has a good control effect by combining the 2, 2-a] quinazoline-3a (1H)-carboxylic acid with wuyiencin.

The invention discloses bacillus subtilis subsp.deserticola, nano-selenium synthesis application of the bacillus subtilis subsp.deserticola and application of the bacillus subtilis subsp.deserticola to prevention and treatment of plant virus diseases, and belongs to the technical field of microorganisms. The bacillus subtilis subsp. Deserticola KBD-4 has better selenium tolerance and can reduce selenium salt into nano-selenium, and meanwhile, a bacterial solution and a nano-selenium active bacterial solution which are generated after the strain is fermented have a better inhibition effect on plant viruses such as TMV, CMV, PVY and TSWV, so that the plant virus diseases can be better prevented and treated. Besides, a biological source nano-selenium potassium fertilizer can be prepared by adding a high-potassium trace element additive into the nano-selenium active bacterial liquid, after root irrigation, the disease-resistant and growth-promoting effects on the tomatoes are better, the tomato yield is increased, the nutritional quality of the tomatoes is further improved, selenium-rich agricultural products meeting the national standard are produced, and the method is worthy of popularization and application. The method has good practical application value in production.

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.

This disclosure provides compositions and combinations for treating cancer. The compositions or combinations comprise a plant virus and a natural killer (NK) cell agonist.

A method of treating cancer in a subject that includes administering in situ to the cancer of the subject a therapeutically effective amount of a plant virus or plant virus-like particle in combination with administration of an immune checkpoint therapy to the subject.

A cassette for the delivery of one or more mRNA is provided, the cassette comprising a 5' UTR, a Nodamura replicon, a ribosomal skipping polynucleotide, the one or more mRNA, a plant virus assembly origin polynucleotide ("OAS"), and a 3' UTR. In one aspect, the cassette further comprises a 5'mRNA cap. In a yet further aspect, the cassette does not comprise a poly A tail.

The invention belongs to the technical field of chemical prevention and control of agricultural diseases and insect pests, and provides an antiviral heterocyclic compound. The heterocyclic compound has a relatively good inhibition effect on plant viruses, has passivation, protection and treatment effects after being applied to plants, is superior to a commercial medicament virazole, has good biological activity and has a commercial prospect; the invention also provides a composition containing the heterocyclic compound. When the composition contains ningnanmycin, the two components have a remarkable synergistic effect in a certain proportion range, and the resistance risk of a single agent can be reduced. The invention provides a new medicament for preventing and treating plant viruses, and has important practical application value.

The utility model relates to the technical field of plant virus inoculation devices, in particular to a plant virus friction virus receiving device which comprises a liquid taking box and a friction virus receiving assembly. The liquid taking box is detachably connected with the friction poison receiving assembly; the friction poison receiving assembly comprises a bottom supporting box, the bottom supporting box is movably connected with a poison receiving box, the bottom supporting box is detachably connected with a liquid taking box, and the liquid taking box and the poison receiving box are oppositely arranged; a plurality of bristles are arranged on the inner wall of the poison receiving box, and the length of the bristles is equal to the depth of the liquid taking box; a movable bottom plate is arranged in the poison receiving box, the movable bottom plate can move towards or away from the liquid taking box, and the bristles movably penetrate through the movable bottom plate. According to the friction virus receiving device provided by the utility model, the friction stress of the blades can be strictly controlled, and the stress of the whole blades is uniform, so that the condition of insufficient friction or excessive force during inoculation is avoided. The device is suitable for laboratory novice, and can reduce the number of times of experiment groping and failure.

The invention relates to the technical field of nitrogen heterocyclic compounds, in particular to nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the specification, wherein X is selected from any one of-OCH3,-OCH2CH3 and-O (CH2) 2CH3. An antiviral activity test result shows that the azacyclo-alkaloid series compounds provided by the invention have antiviral activity, and the antiviral activity level of part of azacyclo-alkaloid reaches or even exceeds that of a positive control drug. Therefore, the azacyclo-alkaloid is expected to be applied to prevention and treatment of plant viruses.

The application relates to the technical field of nitrogen heterocyclic compounds, in particular to a nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the description, X is selected from any one of -OCH3, -OCH2CH3 and -O(CH2)2CH3. The anti-virus activity test result shows that the nitrogen heterocyclic alkaloid series compounds provided by the application all have anti-virus activity, wherein the anti-virus activity level of part of the nitrogen heterocyclic alkaloids reaches or even exceeds that of a positive control drug. Therefore, the nitrogen heterocyclic alkaloid can be expected to be applied to the prevention and treatment of plant viruses.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for transferring an exogenous nucleic acid fragment into a plant by using a plant virus. The technical problem to be solved by the invention is to provide a new method which can be applied to transferring an exogenous fragment into a plant. According to the technical scheme, the method for transferring the exogenous nucleic acid fragment into the plant by using the plant virus comprises the following steps: fusing the exogenous nucleic acid fragment into a 3'non-coding RNA region of TVMVwt to obtain a fused fragment, constructing the fused fragment onto an infectious clone skeleton vector, and transforming the plant. According to the invention, an exogenous coding sequence is inserted into a 3'non-coding RNA region of TVMVwt to construct an infectious cloning vector, the infectious cloning vector is transferred into a plant, and expression of the exogenous coding sequence is realized.

The present invention relates to a highly effective, broad-spectrum anti-plant viral disease pharmaceutical composition and its application. The active ingredients of the anti-plant viral disease pharmaceutical composition include chlorin iron and lentinan; wherein the weight ratio of chlorin iron to lentinan is 1:6000 to 4:1. When a certain concentration of the chlorin iron and lentinan pharmaceutical composition is applied to plants, the two active ingredients exert a synergistic effect, alleviating symptoms of diseased plants, reducing disease indexes, and reducing viral accumulation in the plants. The plant anti-viral disease pharmaceutical composition is a biological agent that is environmentally friendly, safe, and environmentally friendly. It has important application prospects in combating plant viral diseases, preventing and controlling plant diseases caused by plant viruses, and improving plant quality.

The invention provides a coumoxystrobin compound composition for preventing and treating plant virus diseases and application of the coumoxystrobin compound composition, and relates to the technical field of pesticides, the coumoxystrobin compound composition comprises coumoxystrobin and an antiviral synergist, and the mass ratio of the coumoxystrobin to the antiviral synergist is 1: 20-20: 1; the antiviral synergist comprises at least one of ningnanmycin, amino-oligosaccharin or moroxydine hydrochloride, the antiviral effect of coumoxystrobin can be improved by compounding the antiviral synergist and coumoxystrobin, and the combination of the two has a remarkable synergistic effect; through the combination of different action mechanism components, the drug resistance risk of viruses to a single medicament is reduced, and the service life of the medicament is prolonged; the bactericidal composition has a good control effect on various plant virus diseases, and can be widely applied to various crops such as tobacco, tomatoes, cucumbers, peppers and the like. The technical problem that in the prior art, coumoxystrobin is poor in prevention and treatment effect on plant virus diseases is solved.

The invention provides a vector and a method for expressing astaxanthin by using nicotiana benthamiana leaves, which are characterized in that plant virus expression vectors of calendula astaxanthin synthesis related genes AdKC and AdKeto and plant virus gene silencing (RNAi) vectors of nicotiana benthamiana carotenoid cleavage dioxygenase 1 (CCD1) are respectively constructed; the agrobacterium tumefaciens of the three vectors are mixed and infiltrated into the boeng tobacco leaves to obtain the leaves for expressing the astaxanthin. According to the invention, the expression of the nicotiana benthamiana CCD1 gene is interfered while the AdKC and AdKeto genes are expressed in the nicotiana benthamiana leaves, so that the content of astaxanthin expressed in the nicotiana benthamiana leaves is increased.

A method of treating cancer in a subject that includes administering in situ to the cancer of the subject a therapeutically effective amount of a plant virus or plant virus-like particle in combination with administration of an immune checkpoint therapy to the subject.

The invention relates to the technical field of botanical pesticides, and discloses a cistanche polysaccharide anti-plant virus agent as well as a preparation method and application thereof. The preparation is prepared into an aqueous solution by taking cistanche polysaccharide as an effective component and adding an auxiliary agent, and specifically comprises the following components in percentage by weight: 10-40% of a cistanche polysaccharide extract, 0-10% of an organic solvent, 1-20% of a surfactant, 1-5% of an anti-freezing agent, 1-5% of a preservative and the balance of water, and the sum of the weight percentages of the raw materials is 100%. The desertliving cistanche polysaccharide antiviral agent can be used for preventing and treating virus diseases such as ToBRFV and TMV on crops such as tomatoes, tobaccos and peppers, and healthy growth of the crops is guaranteed. The antiviral agent provided by the invention has the advantages of good effect, no drug resistance risk, no residual risk, high safety to non-target organisms, and ecological friendliness, and has the application prospect of industrially preparing green plant protection products.

The invention provides qRT-PCR (quantitative real-time polymerase chain reaction) primers, a kit and a detection method for detecting tomato brown wrinkled fruit virus with high sensitivity, and belongs to the technical field of plant virus detection. The qRT-PCR primer for detecting the tomato brown wrinkled fruit virus, disclosed by the invention, is as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The invention also provides a qRT-PCR detection method of the tomato brown wrinkled fruit virus, the primer and the detection method provided by the invention have the characteristics of strong specificity and high sensitivity, the detection limit is 1.562 * 10 < 1 > copies / [mu] L, and the primer and the detection method have a better detection effect on a sample with low virus loading amount; and a technical support is provided for detecting the tomato brown wrinkled fruit virus and preventing and controlling the tomato brown wrinkled fruit virus.

This invention relates to plant genetic engineering, and more particularly to a variegated Phalaenopsis orchid and its preparation method, the preparation method comprising the following steps: (1) according to the target gene with the base sequence shown in SEQ ID No. 1 PePDS (1) Synthesize specific nucleotide fragments; (2) Construct specific nucleotide fragments into a plant virus vector; (3) Introduce the recombinant plant virus vector obtained in step (2) into Phalaenopsis orchid plant material; (4) Screen to obtain Phalaenopsis orchids with silenced target genes. This invention achieves rapid, efficient, and non-transgenic leaf color variegation creation in commercial Phalaenopsis orchid varieties for the first time through VIGS technology; it not only breaks through the technical bottleneck of difficult and long cycle of genetic manipulation of orchid plants, significantly shortening the trait verification cycle, but also induces diverse and highly ornamental variegated leaf phenotypes such as albino, yellow, or purple in different varieties, and has the advantages of simple operation, low cost, and industrialization feasibility.

The invention relates to the technical field of plant growth regulation, in particular to application of berberine based on ACAA targets in resisting plant viruses and promoting plant growth. The invention reveals that berberine plays a role by specifically targeting acetyl coenzyme A acyltransferase and up-regulating the expression of the acetyl coenzyme A acyltransferase for the first time. The clear compound-target-pathway mechanism overcomes the defect that the existing plant source product mechanism is unknown. As the target spot is clear, the application dosage can be accurately controlled, so that the stability and predictability of field antiviral and growth promoting effects are ensured, and a foundation is laid for large-scale agricultural application. An ACAA target is activated by berberine, and an antiviral defense mechanism and an energy metabolism growth pathway of a plant can be synchronously started, so that the plant growth is effectively promoted while virus diseases are prevented and treated. The disease resistance-growth negative correlation problem existing in agricultural production for a long time is solved, and the dual requirements of modern agriculture for precise prevention and control and yield increase and quality improvement are met.

The invention discloses chloroindole hydrazide-loaded nanogel and a preparation method and application thereof, and relates to the technical field of pesticides, the chloroindole hydrazide-loaded nanogel is successfully prepared, CHI can be stably loaded, slow release is realized, and the chloroindole hydrazide-loaded nanogel has excellent leaf adhesiveness, can resist rain wash and avoid loss of pesticide effect, and can be used as a pesticide for preventing and treating diseases. The application pain point of CHI and the defects of a traditional nano-carrier are overcome; the nano-scale characteristic assists plant cell endocytosis and uptake, breaks through the transport limitation of cell membranes, and gives full play to the high induced resistance activity of CHI. High-speed centrifugal purification is not needed in the preparation process, the process is simplified, the yield is increased, large-scale production is adapted, and meanwhile energy consumption and cost are reduced. Compared with a traditional nano-carrier, the nano-gel does not need expensive precursors or complex modification, is good in dispersity and free of the agglomeration problem, preparation is low in energy consumption and pollution, the comprehensive performance is more suitable for practical application of pesticide carriers, and a solution with practicability and economical efficiency is provided for efficient prevention and treatment of plant virus diseases.

The invention discloses a virus-free cutting seedling planting device and a virus-free cutting seedling planting method for viburnum macranthum, and relates to the field of cutting planting devices. A virus-free cutting seedling planting device for viburnum verum comprises a moving trolley and further comprises a storage box which is installed on the moving trolley, a plurality of sets of magnetic suction plates are connected into the storage box in a sliding mode, the storage box is divided into a plurality of cavities through the magnetic suction plates, and the cavities are used for containing potato seedlings; the push plate is used for pushing the potato seedlings in the chamber; through the arrangement of the storage box, to-be-cut potatoes with seedling raising trays can be temporarily stored, meanwhile, through the cooperation of a push plate, a magnetic suction plate and a communicating pipe, potato seedlings can be moved out and fall into a field below, through the arrangement of a camera, the potato seedlings are shot, the development condition of the potatoes is checked, and the potato seedlings can be conveniently taken out. And the potato seedlings are moved out through the communicating pipe or the auxiliary pipe according to the inspection result, so that the potato seedlings can be conveniently planted.

A scientifically formulated blend of extracts derived from various terrestrial and aquatic plants. It empowers plants to combat both biotic and abiotic stresses by activating their innate resistance mechanisms, resulting in increased overall physiological functions and productivity. The formulation is having different combination PP-1 and PP-2 Blends (blend of Salicylic acid, gibberellic acid and organic silica along with seaweed extract, fulvic substances, humic acid and micronutrients) are prepared for the treatment against Papaya Ring Spot Virus (PRSV) in crops like papaya and tomato, pomegranate, black pepper, pulses, oilseeds, flowers, and others

The invention discloses bacillus subtilis subsp.deserticola, nano-selenium synthesis application of the bacillus subtilis subsp.deserticola and application of the bacillus subtilis subsp.deserticola to prevention and treatment of plant virus diseases, and belongs to the technical field of microorganisms. The bacillus subtilis subsp. Deserticola KBD-4 has better selenium tolerance and can reduce selenium salt into nano-selenium, and meanwhile, a bacterial solution and a nano-selenium active bacterial solution which are generated after the strain is fermented have a better inhibition effect on plant viruses such as TMV, CMV, PVY and TSWV, so that the plant virus diseases can be better prevented and treated. Besides, a biological source nano-selenium potassium fertilizer can be prepared by adding a high-potassium trace element additive into the nano-selenium active bacterial liquid, after root irrigation, the disease-resistant and growth-promoting effects on the tomatoes are better, the tomato yield is increased, the nutritional quality of the tomatoes is further improved, selenium-rich agricultural products meeting the national standard are produced, and the method is worthy of popularization and application. The method has good practical application value in production.

The invention belongs to the technical field of plant virus detection, and particularly relates to an RT-RPA-LFD method capable of rapidly and visually detecting three pathogens of sugarcane mosaic disease at the same time and application of the RT-RPA-LFD method. The invention relates to an RT-RPA-LFD primer group capable of simultaneously, rapidly and visually detecting three pathogens of sugarcane mosaic disease. The RT-RPA-LFD primer group comprises a primer group I, a primer group II and a primer group III, wherein the primer group I comprises an SrMV primer pair and an SrMV probe; the primer group II comprises an SCSMV primer pair and an SCSMV probe; and the primer group III comprises an SCMV primer pair and an SCMV probe. According to the multiple RT-RPA-LFD detection method disclosed by the invention, complicated RNA extraction, cDNA reverse transcription, gel electrophoresis and the like are not needed in the whole process, and multiple detection results can be observed by naked eyes within 5 minutes through a lateral flow test strip only by incubating at the constant temperature of 37 DEG C for 10 minutes. The detection system has the characteristics of rapidness, high efficiency, high sensitivity, strong specificity and no dependence on laboratory equipment, is suitable for on-site large-scale detection of field samples and sugarcane introduction materials, and greatly improves the detection efficiency.

The invention relates to an indole diazepine derivative as well as a preparation method and application thereof, the structural formula is as shown in formula I. Anti-plant virus activity test on the indole diazepine derivative proves that the indole diazepine derivative has good antiviral activity on pepper mild mottle virus. The compound can be used for preparing a pepper mild mottle virus disease prevention and control medicament, and the preparation method is simple and low in production cost.

The invention discloses a pH-responsive isoGPBG hydrogel and an application of the pH-responsive isoGPBG hydrogel in miRNA delivery, and belongs to the technical field of biopesticides. The pH-responsive isoGPBG hydrogel disclosed by the invention is formed by deprotonating isoguanosine and guanosine under an alkaline condition and cross-linking the deprotonated isoguanosine and guanosine with a boric acid group of PBA to form a pH-sensitive phenylboronic acid ester bond. The hydrogel delivery system can break through plant miRNA transmembrane transport obstacles, and efficient delivery and controllable release of antiviral nucleic acid drugs are achieved. When the hydrogel delivery system is used for delivering miRNA, in-vitro degradation of miRNA can be prevented, and the hydrogel delivery system has an important application prospect in prevention and treatment of plant viral diseases and bacterial diseases.

The present invention provides intravenous pharmaceutical compositions comprising engineered plant virus-based nanoparticles for the treatment or prevention of autoimmune conditions. The nanoparticles display immunodominant peptides that induce immune tolerance in a subject when administered intravenously. The invention further provides methods of manufacturing virus-based nanoparticles, including production in plant expression systems and optional viral inactivation, to render them suitable for therapeutic use in mammals. Also provided are methods of intravenous administration of the pharmaceutical compositions to a subject in need thereof for treating or preventing autoimmune conditions.

The application provides a kind of 3, 4-dichloroisothiazole benzamide derivatives and preparation method and purposes thereof, specifically relates to a kind of derivatives, and the chemical structure general formula is seen formula I: the application discloses the general formula of the structure of the above compound, synthesis method and as insecticide, acaricide, fungicide, anti-plant virus agent, plant activator purposes, it is combined with agricultural acceptable adjuvant or synergist and with commercial insecticide, acaricide, fungicide, anti-plant virus agent, plant immune activator in the prevention and treatment of agricultural, forestry, horticultural plant pest, mite damage, disease, viral disease purposes and preparation method.