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491 results about "Plant virus" patented technology

Plant viruses are viruses that affect plants. Like all other viruses, plant viruses are obligate intracellular parasites that do not have the molecular machinery to replicate without a host. Plant viruses can be pathogenic to higher plants.

Seedling method for tissue culture and rapid breeding of camellia semiserrata chi

The present invention discloses a seedling method for tissue culture and rapid breeding of camellia semiserrata chi. The method comprises: explant collecting, aseptic differentiation, strong seedling culturing and bagged seedling transplanting, wherein the explant is provided for breeding. According to the method, bud tip or leaf is adopted as the explant for the breeding, and is subjected to treatments of disinfection and impurity removing; a differentiation culture media is adopted to culture the treated bud tip or the treated leaf to form a small plant; the induced small plant is cut, and inoculated in a new differentiation culture media to carry out multiple subcultures; the resulting small plant is inoculated on the specially-made strong seedling culture media to carry out culture; finally the bagged seedling transplanting is performed. According to the present invention, the original culture media component is adopted; the culture media adopts the oriented polypropylene film bagas the culture dish; the tea seedlings breed rapidly; a lot of plant viruses can be removed, such that the original fine varietal characters can be maintained; the seedling has characteristics of good quality, high transplanting survival rate, rapid flowering, rapid fruiting, high oil yield, high oil quality and long high yield period; the method of the present invention has characteristics of strong operability, high application value and originality, and provides a new approach for the camellia oleifera seedling breeding, wherein the approach has characteristics of rapid breeding and rapid commissioning.
Owner:张桂琴 +3

Infectious clone vector of pepper mild mottle virus, agrobacterium tumefaciens strain, method for preparing same and application of infectious clone vector

The invention relates to the field of genetic engineering, in particular to preparation of an infectious clone vector of a Pepper mild mottle virus (PMMoV) of RNA (ribonucleic acid) plant viruses and tobacco mosaic viruses, pathogenesis analysis on an infectiously cloned agrobacterium tumefaciens strain with the pepper mild mottle virus and exogenous proteins infectiously cloned and expressed by the aid of the PMMoV. The preparation, the pathogenesis analysis and exogenous proteins have the advantages that genomes of Shanghai bay isolate of the pepper mild mottle virus are constructed onto efficient plant expression vectors pCB301-CH by the aid of a genetic recombination process, the recombinant vectors are transferred into the agrobacterium tumefaciens strain by means of electroporation, and accordingly the virus vector with infection ability can be obtained; the plant expression vectors can be constructed by means of infectious cloning, and the exogenous proteins can be efficiently and stably expressed in host plants; mature systems can be provided for studying structures and functions of the virus genomes and studying interaction between the virus and hosts.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Double amide derivative containing sulfur ether and N-sulfur cyano (sulphone) imine structure and replacing pyrazolecarboxamide and preparing method and purpose thereof

The invention provides a double amide derivative containing a sulfur ether and N-sulfur cyano (sulphone) imine structure and replacing pyrazolecarboxamide and a preparing method and purpose thereof, and relates to double amide derivatives containing the pyrazolecarboxamide. The double amide derivative containing the pyrazolecarboxamide has a chemical structure general formula as shown in the map I. The structure general formula and the synthetic method of the double amide derivative and the purpose for being used as insecticides, antiseptics, a plant virus resistance agent and a plant activator are disclosed. The double amide derivative containing the sulfur ether and N-sulfur cayano (sulphone) imine structure and replacing the pyrazolecarboxamide can be mixed with auxiliaries or synergists which are acceptable in agriculture to form a processing technology to be used for preparing the insecticides, the antiseptics, the plant virus resistance agent and the plant activator. The invention further discloses the purpose and preparing method of the double amide derivative containing the sulfur ether and N-sulfur cyano (sulphone) imine structure and replacing the pyrazolecarboxamide combining with commodity insecticides, commodity antiseptics, commodity plant virus resistance agents and commodity plant activators to be used for preventing and curing plant diseases, insect pests, virus diseases in agriculture, forestry and horticulture.
Owner:NANKAI UNIV

Primer and method for carrying out specific detection and absolute quantification on tomato spotted wilt virus

The invention relates to a primer and a method for carrying out specific detection and absolute quantification on a tomato spotted wilt virus and belongs to the field of plant virus detection. According to the primer and method disclosed by the invention, the tomato spotted wilt virus is taken as an object of study, a more rapid, simple, accurate and sensitive method for quantitatively detecting a plant virus is established, the method comprises the following steps of (1) establishing a standard curve according to the standard plasmid; (2) obtaining the cDNA of a sample to be detected; synthesizing cDNA by virtue of reverse transcription; and carrying out fluorescence quantitative PCR by virtue of using cDNA as a template and TSWV-113F / TSWV-113R as a primer to obtain the Ct value of the sample to be detected; and (3) calculating the copy number of the tomato spotted wilt virus in the sample to be detected by virtue of the standard curve. By virtue of the method, tomato spotted wilt virus within the sample can be accurately quantified in 4-5 hours, the time is saved and the experimental steps are simplified, the method is contributed to taking corresponding remedial measures before the onset of plant diseases to prevent huge economic losses brought by the widespread of the virus among crops.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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