499 results about "Plant virus" patented technology

The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

The invention relates to an anti-plant virus fertilizer additive and a fertilizer containing the additive. And the additive contains medium trace element-containing fertilizer, organic acids, chitin-containing matters, and plant virus preventing and curing agent. As used, the additive has remarkable production increasing effect, where the production of the grain crops is averagely increased by 7-10%, that of the vegetables by 15-30%, that of the fruits by 12-18%, and that of the cotton by 6-12%; besides, the additive can prevent and cure some plant viruses.

Methods of inducing systemic acquired resistance to infection in a plant are provided. The methods comprise applying a composition comprising a Bacillus control agent to said plant wherein said plant is capable of producing defense proteins. Also provided are, methods for controlling one or more plant diseases, methods for preventing plant virus transmission, methods for preventing and / or treating soil-borne plant pathogens using the Bacillus control agent of the present invention, and methods of generating bacterial spores. In addition, synergistic biocontrol combinations and methods of using the same are provided.

The invention provides o-formammidotiazol-benzamide derivatives, and a preparation method and applications thereof. The o-formammidotiazol-benzamide derivatives have the chemical structure general formula represented by the formula I or the formula II. The invention discloses the structure general formula of the compounds, the synthesis method and the applications of the compounds as insecticides, acaricides and bactericides; the compounds are mixed with agriculturally acceptable auxiliary agents or synergistic agents for use in processing crafts of preparing the insecticides, the acaricides and the bactericides. The invention also discloses the applications of the compounds in combination with the commercial insecticides, the acaricides, the bactericides, anti-plant virus agents and plant activators for prevention and treatment of agriculture, forestry, horticulture plant diseases, insect attacks, acarus attacks and virus diseases, and the preparation method of the compounds.

The invention provides 1,2,3-thiadiazole-1,3,4-thiadiazole derivatives, and a preparation method and application thereof. The invention relates to 1,2,3-thiadiazole-containing heterocyclic compounds with the chemical structural general formula shown as the specification. The invention discloses the structural general formula of the compounds, a synthesizing method of the compounds, application ofthe compounds serving as pesticides, bactericides, anti-plant-virus agents and plant activating agents, and a process for preparing the pesticides, the bactericides, the anti-plant-virus agents and the plant activating agents by mixing the compounds and agriculturally acceptable adjuvants or synergists. The invention also discloses application of the compounds when the compounds are combined withcommodities of the pesticides, the bactericides, the anti-plant-virus agents and the plant activating agents in prevention and control of agricultural, forestry, horticultural and health diseases, insect pests and virus diseases, and a preparation method of the compounds.

The invention relates to an anti-plant virus fertilizer additive and a fertilizer containing the additive. The anti-plant virus additive contains a medium trace element-containing fertilizer, organic acid, an anti-plant viral agent and a trace element additive. The fertilizer containing the additive is composed of an elemental fertilizer or a compound fertilizer and the anti-plant virus fertilizer additive, and the content of the additive accounts for 0.1-5% of the total fertilizer weight. The method has the advantages that by adopting the anti-plant virus additive or fertilizer, the yield-increasing effect is obvious, and moreover plant viruses can be prevented.

A gene chip for detecting viruses of leguminous plant is disclosed, several probes and nature-control collations are fixed on surface of solidoid carrier, the probes has the nucleotide sequence showed on from SEQ ID No.1 to SEQ ID No.80; the nature-control collations consists of the surface chemistry LUT, fluorescence labeling LUT, positive LUT, negative LUT, cross LUT and blank control. The advantages and innovations of the invention are following: practicability, quick-speed, and idiocrasy, and sensitivity, low activity cost, repeatability, stable and reliable results. The testing system can guarantee the repeatability and accuracy of results that is proofed by the repeatability and stability test, the system can be widely used in area of plant quarantine of the outbound and enter the country test and quarantine system, the safety supervision of plant source food, the diagnosis of plant virus in plant protecting station and the authenticate of pathogenesis in research of plant taxonomic virology. The gene chip testing call for detecting viruses of leguminous plant can be used in about 600 daily quarantine pursuits of leguminous plant source food.

The present invention discloses a seedling method for tissue culture and rapid breeding of camellia semiserrata chi. The method comprises: explant collecting, aseptic differentiation, strong seedling culturing and bagged seedling transplanting, wherein the explant is provided for breeding. According to the method, bud tip or leaf is adopted as the explant for the breeding, and is subjected to treatments of disinfection and impurity removing; a differentiation culture media is adopted to culture the treated bud tip or the treated leaf to form a small plant; the induced small plant is cut, and inoculated in a new differentiation culture media to carry out multiple subcultures; the resulting small plant is inoculated on the specially-made strong seedling culture media to carry out culture; finally the bagged seedling transplanting is performed. According to the present invention, the original culture media component is adopted; the culture media adopts the oriented polypropylene film bagas the culture dish; the tea seedlings breed rapidly; a lot of plant viruses can be removed, such that the original fine varietal characters can be maintained; the seedling has characteristics of good quality, high transplanting survival rate, rapid flowering, rapid fruiting, high oil yield, high oil quality and long high yield period; the method of the present invention has characteristics of strong operability, high application value and originality, and provides a new approach for the camellia oleifera seedling breeding, wherein the approach has characteristics of rapid breeding and rapid commissioning.

This invention discloses a medicine of anti-tumor and anti-plant virus effect---- cyanoacrylate category derivative and its preparing method and biological activity. The following general formula denotes the compound and its preparing method. The definition of R1, R2, R3 in the formula is in instruction book. Materials are carbon bisulfide, cyan acetic ester, dimethyl sulfate, aromatic amine, and sodium cyanide is catalyst, cyanoacrylate category derivative is synthesized by three steps. There is proliferation inhibitory effect of compound b, k to PC3 prostate cancer cell gene stock, and proliferation inhibitory effect of compound i, j to NIN3T3 cell ERK albumen phosphorylation, and good anti-cancer activity is revealed. There is inhibitory activity of compound b, n to tobacco mosaic, and certain anti-plant virus activity is revealed.

The invention discloses an application of an activator protein prepared from Alternaria tenuissima by microbial fermentation to a pesticide preparation. The activator protein from Alternaria tenuissima which is prepared from Alternaria tenuissima by fermentation and special extraction processes is a single and stable protein with biological activities. The protein can be prepared with glucosamine oligosaccharides and related protein protectants into wettable powder, the protein can be combined with a receptor protein on a plant cell membrane, in order to induce increases of a series of related enzymatic activity and gene expression quantity in the plant, stimulate a series of metabolism regulation in the plant, promote growth of roots, stems and leaves of the plant, increase chlorophyll content, and increase crop yield. The wettable powder prepared by the protein from Alternaria tenuissima completely accords with each national index, and has the effects for preventing and controlling plant virus diseases, greatly improving physiological functions of plants, enhancing disease resistance of plants, and increasing crop output to a certain degree.

The invention discloses a myricetin derivative containing amide thioether thiadiazole, of which a general formula is represented as follows, wherein R is hydrocarbyl groups, phenyl groups, substituted phenyl groups, heteroaromatic ring groups or substituted heteroaromatic ring groups. The compound has high treatment and prevention effect against tobacco mosaic virus (TMV), has high anti-plant virus activity and can be used for preparing anti-plant virus pesticides. The compound has high inhibition activity on Xanthomonas axonopodis and Xanthomonas oryzae and can be used for preparing agricultural bactericides.

The invention relates to the field of genetic engineering, in particular to preparation of an infectious clone vector of a Pepper mild mottle virus (PMMoV) of RNA (ribonucleic acid) plant viruses and tobacco mosaic viruses, pathogenesis analysis on an infectiously cloned agrobacterium tumefaciens strain with the pepper mild mottle virus and exogenous proteins infectiously cloned and expressed by the aid of the PMMoV. The preparation, the pathogenesis analysis and exogenous proteins have the advantages that genomes of Shanghai bay isolate of the pepper mild mottle virus are constructed onto efficient plant expression vectors pCB301-CH by the aid of a genetic recombination process, the recombinant vectors are transferred into the agrobacterium tumefaciens strain by means of electroporation, and accordingly the virus vector with infection ability can be obtained; the plant expression vectors can be constructed by means of infectious cloning, and the exogenous proteins can be efficiently and stably expressed in host plants; mature systems can be provided for studying structures and functions of the virus genomes and studying interaction between the virus and hosts.

The invention provides a pesticide composition containing ningnanmycin. The pesticide composition also contains two ningnanmycin analogs which can effectively prevent and control plant virus diseases, fungus diseases and bacteria diseases separately and together. In addition, the invention also provides a preparation method for the pesticide composition, a prepared pesticide preparation and application of the ningnanmycin analogs and the like.

The invention provides a double amide derivative containing a sulfur ether and N-sulfur cyano (sulphone) imine structure and replacing pyrazolecarboxamide and a preparing method and purpose thereof, and relates to double amide derivatives containing the pyrazolecarboxamide. The double amide derivative containing the pyrazolecarboxamide has a chemical structure general formula as shown in the map I. The structure general formula and the synthetic method of the double amide derivative and the purpose for being used as insecticides, antiseptics, a plant virus resistance agent and a plant activator are disclosed. The double amide derivative containing the sulfur ether and N-sulfur cayano (sulphone) imine structure and replacing the pyrazolecarboxamide can be mixed with auxiliaries or synergists which are acceptable in agriculture to form a processing technology to be used for preparing the insecticides, the antiseptics, the plant virus resistance agent and the plant activator. The invention further discloses the purpose and preparing method of the double amide derivative containing the sulfur ether and N-sulfur cyano (sulphone) imine structure and replacing the pyrazolecarboxamide combining with commodity insecticides, commodity antiseptics, commodity plant virus resistance agents and commodity plant activators to be used for preventing and curing plant diseases, insect pests, virus diseases in agriculture, forestry and horticulture.

The present invention relates to methods of imparting virus resistance to plants. In one aspect, this method involves silencing a gene encoding a translation initiation factor eIF4E in the plant. In another aspect, this method involves overexpressing a heterologous translation initation factor eIF4E in a plant. The present invention further relates to a genetic construct containing a nucleic acid molecule encoding a heterologous translation initiation factor eIF4E, as well as to an expression system containing the genetic construct and a host cell transformed with the genetic construct. The present invention also relates to transgenic plants, seeds, and plant parts transformed with the genetic construct. The present invention also relates to an isolated nucleic acid molecule encoding a mutant translation initiation factor eIF4E that is effective in imparting virus resistance in plants. The present invention also relates to a mutant translation initiation factor eIF4E and a method for making the mutant.

The invention provides an o-formylamino benzamide derivative containing N-cyano sulfone(sulfur) imine and a preparation method and use thereof. The invention relates to a bisamide derivative containing pyrazole formamide, wherein the bisamide derivative containing pyrazole formamide has a chemical structural general formula shown in I. The invention discloses structural general formulae and synthesis methods of the compounds, use of the compounds serving as insecticide, bactericide, plant virus resisting agents and plant activators and processing processes for the compounds in the preparation of insecticide, bactericide, plant virus resisting agents and plant activators in a manner of being mixed with agriculturally-acceptable assistants or synergists. The invention further discloses use of the compounds in the prevention and treatment of the diseases, insect pests and viral diseases of agriculture, forestry and horticulture in a manner of being combined with the commercial insecticide, bactericide, plant virus resisting agents and plant activators and preparation methods thereof.

The present invention provides compositions for use as vaccines against the influenza virus, and rapid methods of producing such compositions. The composition include i) at least one peptide derived from an influenza virus, wherein the peptide is fused to a capsid protein derived from a plant virus forming a recombinant capsid fusion peptide and ii) at least one isolated antigenic protein or protein fragment derived from a human or avian influenza virus. The isolated antigenic protein or protein fragment derived from the human or avian influenza virus can be conjugated to the surface of the recombinant capsid fusion peptide.

The present invention relates to a plant virus inhibitory artificial miRNA (microRNA) and construction and application thereof. The invention firstly designs oligonucleotides with excellent silencing effect according to the nucleic acid sequence of a gene silencing suppressor of a plant virus infecting plants. When the virus infects a plant, these oligonucleotides transfected into the plant can form miRNA in the plant which specially binds to the corresponding parts of mRNA (massager RNA) of the virus gene silencing suppressor to affect the transcription of the mRNA, thereby achieving virus inhibitory effect. In addition, oligonucleotides of the invention are optimized and designed on the basis of locating the most conservative positions after comparing the virus gene silencing suppressors derived from different virus subtypes, the oligonucleotides designed based on these positions has broad-spectrum resistant effect on related viruses after being introduced into the plant.

The present invention relates to one kind of plant viral disease preventing and controlling agent. It contains the composition of moroxydine hydrochloride, copper compound and azone disinfectant as its active component.

The invention provides formyl urea derivatives containing 1,2,3-thiadiazole perssad and a preparation method and application thereof, and relates to heterocyclic compounds containing 1,2,3-thiadiazole, which have the chemical construction general formula shown in the specification of the invention. The invention discloses the construction general formula, a synthesis method, application of the compounds used as insecticides, fungicides, anti-plant virus agents and plant activating agents, and the process for preparing insecticides, fungicides, anti-plant virus agents and plant activating agents by mixing the compounds with acceptable auxiliaries or synergists in agriculture, the invention also discloses a preparation method and application of a combination of the compounds and commodity insecticides, fungicides, anti-plant virus agents and plant activating agents in disease and insect prevention and control in agriculture, forestry and gardening.

The invention relates to a rapid virus detection method of monorchid herminium herb virus-free seedlings of tissue culturing, which belongs to the technology field of plant virus detection, including the following steps: preparing the culture medium, stem tip virus-free culturing, virus-free seedling culturing of the indicator plant tomato, micrografting culturing and the proliferation culture of the virus-free seedlings of tissue culturing. The method has the advantages that the adopted culture medium, tissue culturing conditions and the micrograft cutter are applicable to the micrografting culturing of the virus-free seedlings and facilitating the healing of the graft unions with a grafting rate at more than 90%, which fully meet the requirement of the virus detection. Compared with the conventional field indicator plant detection, the method greatly reduces the required detection time, which is free from time and environmental factors and can be carried out under the laboratory conditions throughout the year. The method is very practical and applicable to the rapid virus detection of the monorchid herminium herb virus-free seedlings and the mass production of the virus-free seedlings.

The present invention provides an anti-plant virus agent which exhibits preventative and therapeutic effects on plant virus diseases. The anti-plant virus agent of the present invention is characterized by containing at least one compound selected from the group consisting of ascorbic acid derivatives represented by Formula (I) (wherein, R1 to R4 each independently represents a hydrogen atom, —SO3H, —PO3H2, a glycosyl group, or —COR11, wherein R11 represents an unsubstituted or substituted C1-30 alkyl group, or an unsubstituted or substituted C2-30 alkenyl group, with the proviso that R1 to R4 may not all be hydrogen atoms at the same time), and salts thereof.

Methods of inducing systemic acquired resistance to infection in a plant are provided. The methods comprise applying a composition comprising a Bacillus control agent to said plant wherein said plant is capable of producing defense proteins. Also provided are, methods for controlling one or more plant diseases, methods for preventing plant virus transmission, methods for preventing and / or treating soil-borne plant pathogens using the Bacillus control agent of the present invention, and methods of generating bacterial spores. In addition, synergistic biocontrol combinations and methods of using the same are provided.

The invention discloses a composition for preventing and treating plant nematode and virus diseases, and an application of the composition, belonging to the technical field of biopesticide. The composition takes chitosan oligosaccharide and lentinan as active ingredients and can be made into liquid or effervescent tablets. The composition can be used for separately and simultaneously preventing and treating the plant nematode and the virus diseases of plants, and has good using effect on pumpkins, winter squashes, pepper, alfalfa, lily, strawberries and gingers, thus effectively reducing the crop diseases and pest and improving the yield and the quality of crops.

The invention relates to gossypol derivatives and preparation method thereof and application of the gossypol derivatives in plant virus prevention and control, insecticidal action, bactericidal action and anti-cancer action, wherein the significance of all groups in a formula is shown in the description. The gossypol derivatives show the excellent anti-plant virus activity, bactericidal activity, insecticidal activity and anti-cancer activity.

The invention relates to a primer and a method for carrying out specific detection and absolute quantification on a tomato spotted wilt virus and belongs to the field of plant virus detection. According to the primer and method disclosed by the invention, the tomato spotted wilt virus is taken as an object of study, a more rapid, simple, accurate and sensitive method for quantitatively detecting a plant virus is established, the method comprises the following steps of (1) establishing a standard curve according to the standard plasmid; (2) obtaining the cDNA of a sample to be detected; synthesizing cDNA by virtue of reverse transcription; and carrying out fluorescence quantitative PCR by virtue of using cDNA as a template and TSWV-113F / TSWV-113R as a primer to obtain the Ct value of the sample to be detected; and (3) calculating the copy number of the tomato spotted wilt virus in the sample to be detected by virtue of the standard curve. By virtue of the method, tomato spotted wilt virus within the sample can be accurately quantified in 4-5 hours, the time is saved and the experimental steps are simplified, the method is contributed to taking corresponding remedial measures before the onset of plant diseases to prevent huge economic losses brought by the widespread of the virus among crops.

The invention relates to an antiviral composition containing daucosterol and sitosterol and antiviral application of the antiviral composition. The weight ratio of daucosterol to sitosterol in the antiviral composition is 1:50 to 50:1. Compared with single use of daucosterol or sitosterol, the antiviral composition disclosed by the invention has a significant synergistic effect on a variety of plant viruses, and has the characteristic of long lasting period at the same time, so that the service life of an active compound is prolonged, and the generation of drug resistance of the plant viruses is delayed.