203 results about "GMO Plants" patented technology

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention belongs to the technical field of agricultural biology, and particularly relates to a plant constitutive promoter OsSULTR2. 2pro and an application thereof. The promoter is characterized in that the promoter is OsSULTR2; the nucleotide sequence of the 2pro is as shown in SEQ ID NO. 1. The promoter provided by the invention is OsSULTR2; 2pro is a rice endogenous constitutive promoter, can drive a target gene to realize efficient and stable expression in plant callus, roots, stems, leaves, young ears, seeds and other tissues in a vegetative growth period, and can replace an existing non-plant-source promoter. The method has important application value in the field of plant genetic engineering, and can effectively reduce potential safety risks of transgenic plants caused by introduction of exogenous DNA.

The present invention relates to a recombinant construct comprising: a first expression cassette comprising a fluorescence enhancing factor; wherein the amino acid sequence of the fluorescence enhancement factor is as shown in SEQ ID NO: 2, or an amino acid sequence which at least has 60%, 65%, 70%, 75%, 80%, 85% or 90% identity with the SEQ ID NO: 2 and is functionally equivalent to the protein as shown in the SEQ ID NO: 2; the first expression cassette is configured to increase the self-luminous intensity of a plant, plant tissue, or cell. According to the application, the LumEnhancer gene is further introduced on the basis of an FBP biological self-luminous system, so that the physiological activity of transgenic plant cells is effectively improved, and the energy metabolism level of the plant is further enhanced, thereby providing a more sufficient energy basis for a bioluminescence reaction and effectively enhancing the luminous intensity of the whole plant.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention relates to the technical field of plant breeding, in particular to a primer combination for detecting a regulatory element and an exogenous gene of a transgenic plant and application of the primer combination. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. 22-63. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. The application comprises: (1) transgenic plant variety germplasm supervision or transgenic plant component screening in non-transgenic varieties; (2) screening transgenic ingredients of the plant product; (3) auxiliary screening in the plant breeding process; and (4) tracing plant varieties. The invention provides a primer combination which can be applied to detection of regulatory elements and exogenous genes of transgenic plants, can realize detection of a plurality of regulatory elements and exogenous genes in a reaction system, and can meet high-throughput screening of components of the transgenic plants. The method is used for monitoring illegal planting of transgenic plants and detecting transgenic components of related plant products.

The present application relates to the technical field of intelligent identification system of crop pests, in particular to a plant field pest fine-grained identification method, system, device and storage medium based on deep learning. The identification method provided by the present application is specialized in high-precision identification of real field scenes, and can provide technical support for important work such as future development of field inspection robot, automatic identification and monitoring system of field pests and the like. In addition to pest monitoring, the field biological safety test of genetically modified plants is gradually carried out at present, and by using the identification method provided by the present application, the dynamic change of farmland insect community can be quickly and accurately identified and predicted, so that the efficiency and accuracy of ecological investigation are greatly improved.

The application discloses a protein separated from Pinus tabulaeformis Carr. Pinus tabuliformis ​ and an encoding gene PtAMT2.8 of the protein, which can significantly enhance nitrogen absorption and transportation of plants. 31019b The PtAMT2.8 gene is introduced into a yeast mutant strain with a deficiency in ammonium nitrogen absorption function, so that the growth phenotype of the yeast mutant is recovered, and the PtAMT2.8 gene is introduced into Pinus tabulaeformis and Arabidopsis thaliana, so that the nitrogen absorption and transportation capacity of the transgenic plants can be significantly enhanced. The application has important significance for accelerating genetic improvement of plants and cultivating new varieties with high yield, high quality and multi-resistance.

The invention discloses a protein which is separated from pinus tabuliformis and is capable of remarkably enhancing nitrogen absorption and transport of a plant, and a coding gene PtAMT2.8 of the protein. The PtAMT2.8 gene is introduced into a yeast ammonium nitrogen absorption function deficient mutant strain 31019b, so that the growth phenotype of a yeast mutant can be recovered, and the nitrogen absorption and transport capability of a transgenic plant can also be remarkably enhanced by introducing the PtAMT2.8 gene into pinus tabulaeformis and a model plant arabidopsis thaliana. The invention has important significance in accelerating plant genetic improvement and cultivating high-yield, high-quality and multi-resistant new varieties.

The present invention relates to a method for producing diosmin in plants by reconstructing the diosmin biosynthetic pathway. Reconstructing the diosmin biosynthetic pathway by selecting optimal genes according to the present invention has the effect of enabling mass production of diosmin or a precursor thereof in plants, and therefore can be advantageously used for large-scale production of diosmin or a precursor thereof in the form of a gene expression cassette, a recombinant vector, a transformant, a transgenic plant, a method for producing a transgenic plant, or a method for producing diosmin or a precursor thereof.

The present disclosure provides a transgenic plant comprising one or more nucleotide sequences encoding polypeptides selected from photosystem II subunit S (PsbS), zeaxanthin epoxidase (ZEP), and violaxanthin de-epoxidase (VDE), operably linked to at least one expression control sequence. Expression vectors for making transgenic plants, and methods for increasing biomass production and / or carbon fixation and / or growth in a plant comprising increasing expression of at least one of PsbS, ZEP and VDE polypeptides are also provided.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The present invention relates to a method for preparing a MAGEA8-Fc transgenic plant. Through a platform using a tobacco plant, a recombinant protein MAGEA8-Fc, in which a human IgG Fc fragment is fused to a MAGEA8 protein, can be stably expressed. The method of the present invention enables high-level expression efficiency and stable protein production by introducing a target gene into a plant genome using an Agrobacterium binary vector. By directly isolating and purifying the recombinant protein from the transgenic tobacco plant, large-scale production can be easily achieved. Compared to conventional microbial or animal cell-based expression systems, the method provides economic efficiency and scalability, and thus can be usefully applied as a platform for producing fusion proteins.

The invention belongs to the field of molecular biology and gene engineering, and particularly relates to a construction method of a transgenic plant, a biological material and application of the biological material. The invention provides a construction method of a transgenic plant, which comprises the step of regulating and controlling the expression level of an OsRLK7 gene in a receptor plant to obtain an OsRLK7 gene overexpressed receptor plant. By improving the expression level of the OsRLK7 gene in a receptor plant, the grain size and thousand grain weight of the receptor plant can be effectively changed, so that the yield of the receptor plant is improved. The OsRLK7 gene is used as a positive regulation factor, and the change of the expression level of the OsRLK7 gene directly affects the development process of receptor plant seeds so as to further affect the size and thousand seed weight of the seeds. The regulation and control mechanism provides a new thought and means for gene function research and breeding work related to the yield of plants such as rice and the like, and has important theoretical value and application prospect.

The present invention addresses the problem of providing a technique capable of haploid induction by enhancing the expression and / or function of a regulatory gene. The problem is solved by the use of a modified plant comprising an introduced or modified H2A.W gene, wherein said introduction or modification enhances the expression and / or function of the H2A.W gene in a germ cell.

The application discloses a protein kinase gene positively regulating drought resistance of oats AVESA.00022b.r1.6C0001455 and application thereof, and belongs to the technical field of plant genetic engineering. AVESA.00022b.r1.6C0001455 The CDS sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. Expression mode analysis shows that the gene is significantly induced and up-regulated under drought stress; virus-induced gene silencing verification shows that silencing the gene can significantly reduce the drought resistance of the oats; overexpression verification shows that overexpression of the gene can significantly enhance the drought resistance of the oats, and the performance is that wilting is delayed, the water loss rate is reduced, and the survival rate is improved. The application first proves that the gene is a key positive regulation factor of drought resistance in the oats, and can be used for cultivating drought-resistant transgenic plants and as a drought-resistant molecular marker for assisted breeding, and has important application value in genetic improvement of crop drought resistance.

The present invention relates to a method for cultivating transgenic plants having increased anthocyanin content and enhanced tolerance to biotic and abiotic stress environments. The method comprises transferring a bHLH35 gene into recipient plants using a genetic engineering transformation method, to obtain transgenic plants having increased anthocyanin content and tolerance to biotic and abiotic stress environments. The bHLH35 gene may be selected from a ChbHLH35 gene from Corydalis hemidicentra or an AtbHLH35 gene from Arabidopsis thaliana.

Methods and materials for modulating biomass composition in plants are disclosed. For example, nucleic acids encoding biomass composition-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having altered biomass composition and plant products produced from plants having altered biomass composition.

The invention belongs to the technical field of gene engineering, and particularly relates to a poa pratensis drought-resistant gene PpNRT2.4 and application thereof. The invention provides a nitrate transporter gene PpNRT2.4 from poa pratensis, provides a complete coding sequence of the nitrate transporter gene PpNRT2.4 and a protein sequence coded by the nitrate transporter gene PpNRT2.4, clarifies biological functions of the PpNRT2.4 gene, and verifies that the drought resistance of transgenic plants can be remarkably improved by overexpression of the PpNRT2.4 gene. A precise, efficient and safe innovative technical path is provided for creating new drought-resistant germplasm of turfgrass and other crops and breaking through the traditional breeding efficiency bottleneck.

The present disclosure relates to the Field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure aiso provides herbicide tolérant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molécules, and methods of using the same.

The invention discloses a device and method for evaluating the influence of a transgenic plant on a soil microbial community in the technical field of transgenic plant planting evaluation, and the device comprises a base, a box body is arranged on the base, regulation and control equipment is arranged on the box body, and the interior of the box body is used for planting the transgenic plant; the regulation and control equipment is used for changing the planting environment in the box body; the side wall of the box body is provided with an opening and a shielding assembly used for blocking the opening. The sampling opening with the shielding assembly is formed in the side wall of the simulation box, when the soil samples need to be collected, the soil samples with different depths can be directly obtained from the side face of the box body only by removing blocking of the shielding assembly on the opening, and the sampling mode does not need to puncture or excavate soil from the top down; disturbance and damage to a soil surface structure, root system distribution and a microbial microenvironment in a traditional top sampling process are effectively avoided.

The application discloses a soybean alkali-resistant gene GmFPS1 and application thereof. The soybean farnesyl pyrophosphate synthase gene GmFPS1, and a nucleotide sequence of the GmFPS1 gene is shown as SEQ ID NO. 1. The application also discloses application of the soybean farnesyl pyrophosphate synthase gene GmFPS1 in construction of alkali-resistant transgenic crops. The application carries out genetic transformation of a plant alkali-resistance related protein coding gene GmFPS1 into transgenic Arabidopsis and Agrobacterium rhizogenes-mediated soybean chimera, and studies alkali stress resistance of the transgenic Arabidopsis and Agrobacterium rhizogenes-mediated soybean chimera. The transgenic Arabidopsis and Agrobacterium rhizogenes-mediated soybean chimera obtained have improved alkali stress resistance. The result of qRT-PCR also shows that the soybean GmFPS1 is induced in response to alkali stress, and the expression level is significantly up-regulated. Therefore, the plant alkali-resistance related protein coding gene GmFPS1 is introduced into crops by a genetic engineering method, and a new alkali stress-resistant transgenic plant variety can be obtained.

The invention provides a raspberry NFYA3 gene and application of the raspberry NFYA3 gene in raspberry secondary metabolism. The amino acid sequence of the NFYA3 gene is as shown in SEQ ID NO. 1, and the nucleotide sequence for coding the gene is as shown in SEQ ID NO. 2. The gene can regulate and control synthesis of secondary metabolites, especially ellagic acid, in transgenic plants. The overexpressed NFYA3 gene significantly enhances the synthesis of ellagic acid in raspberry, and has wide agricultural and medicinal values.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

The invention relates to the technical field of plant breeding, in particular to application of ZmCIPK9 protein or a coding gene thereof in regulating and controlling heat resistance of plants. The ZmCIPK9 protein comprises an amino acid sequence as shown in SEQ ID NO. 1 (sequence identifier number 1). 1, and the coding gene comprises a nucleotide sequence as shown in SEQ ID NO. 2. The application comprises the step of improving the heat resistance of the plant by improving the expression level of the ZmCIPK9 protein in the plant or the coding gene of the ZmCIPK9 protein. New functions of the ZmCIPK9 protein are obtained through research, and the ZmCIPK9 protein can be used for (1) cultivating transgenic plants; (2) improving plant varieties related to heat resistance; and (3) plant germplasm resource improvement. The discovery of the heat-resistant function of the ZmCIPK9 protein provides new gene targets and resources for cultivating heat-resistant plant varieties, and is of great significance to research on a heat-resistant molecular mechanism of corn.

The invention discloses a transcription factor HbNAC47 related to saline-alkaline tolerance, and a coding gene and application thereof. The amino acid sequence of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 1. The nucleotide sequence of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 2. The transcription factor HbNAC47 or the coding gene thereof is applied to cultivation of saline-alkaline tolerant transgenic plants. The invention further provides a recombinant expression vector, an expression cassette or a recombinant thallus of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character and application of the recombinant expression vector, the expression cassette or the recombinant thallus in cultivation of saline-alkaline tolerant transgenic plants. By overexpressing the barley grass HbNAC47 gene, the expression of salt-alkali tolerant related genes is regulated, so that a salt-alkali tolerant plant is obtained, and the barley grass HbNAC47 gene has great production and application values.

The application discloses a Gossypium hirsutum L. GhISR43 gene, a coding protein thereof and application of the GhISR43 gene in regulating plant salt stress tolerance. The application provides the GhISR43 gene isolated from cotton and capable of regulating plant salt stress tolerance performance, wherein a polynucleotide sequence of the GhISR43 gene is shown as SEQ ID No. 1, and an amino acid sequence of a coding protein of the GhISR43 gene is shown as SEQ ID No. 2. The application provides an expression cassette containing the gene, a recombinant expression vector or a recombinant host cell. The application also provides application of the GhISR43 gene in regulating plant salt stress tolerance performance, including: making the normal function of the GhISR43 gene defective by making the GhISR43 gene in cotton produce a mutation, interfering with the normal expression or normal function of the GhISR43 gene in cotton or increasing the salt stress tolerance performance of cotton; or obtaining a transgenic plant by overexpressing the GhISR43 gene in plants to reduce the salt stress tolerance performance of the plants.

The invention discloses an application of a GhSZF3 gene in improving the salt tolerance of plants. Belongs to the technical field of cotton salt-tolerant gene research. The GhSZF3 gene provided by the invention can improve the salt tolerance of plants, can significantly improve the seed germination ability of transgenic arabidopsis thaliana under salt stress, and is of great significance to cultivation of new varieties of salt stress resistant transgenic plants. A wider gene source can be provided for directionally editing and improving the salt resistance of the cotton and molecular breeding of various plants and crops.

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an m6A demethylase is introduced, wherein said m6A demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an m6A demethylase into a regenarable plant cell, and regenerating a transgenic plant from the regenerable plant cell.