524 results about "GMO Plants" patented technology

The present disclosure is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The disclosure discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The disclosure also discloses methods and compositions for targeted genome editing in the pest in order to achieve a reduction in pest infestation through disruption of protein activity. The disclosure is also directed to methods for making transgenic plants that express the double stranded RNA molecules and targeted genome editing constructs for use in protecting plants from pest infestation.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

The invention discloses a soybean bidirectional promoter and application thereof, and belongs to the technical field of separation and application of bidirectional promoters. The nucleotide sequence of the soybean bidirectional promoter disclosed by the invention is as shown in SEQ ID NO. 2. Experiments prove that the soybean bidirectional promoter can bidirectionally and simultaneously drive the expression of a target gene, and by applying the soybean bidirectional promoter, plant traits can be improved, transgenic plants or new plant varieties can be cultivated, and the biological breeding process can be promoted.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT5 is found for the first time, and the gene has obvious positive correlation with the accumulation of valine (Val), isoleucine (Ile) and leucine (Leu) of crops. The invention provides an important target gene HvBCAT5 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT5 gene as well as a recombinant vector, a recombinant bacterium and a transgenic plant thereof have good application prospects in the fields of food and feed industry.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention relates to the technical field of gene engineering, in particular to OsSULTR3; 1 protein and the coding gene thereof are applied to cultivation of selenium-rich plants. According to the invention, the OsSULTR3 is disclosed for the first time; 1 gene has the function of increasing the selenium content of rice, and OsSULTR3; 1 overexpression results in increase of the selenium content of rice grains. A new direction is provided for breeding of selenium-rich plants such as rice and the like and preparation of transgenic plants, the selenium content in the plants such as rice and the like can be remarkably improved by constructing and transforming the transgenic plants of the gene, the nutritional quality of the plants can be improved, and the gene has a wide application prospect.

The invention relates to the technical field of gene engineering, in particular to a plant low-phosphorus-resistant important gene GsMYB10 and application thereof. Researches show that the expression of the GsMYB10 gene is induced to be up-regulated under the stress of low phosphorus, and under different phosphorus concentration treatment conditions, the over-expression of the GsMYB10 can obviously increase the biomass of a transgenic plant, so that the plant growth is promoted under the low-phosphorus condition; meanwhile, overexpression of GsMYB10 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth.

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.

The invention provides a salt-tolerant gene, namely a PrABC1 gene and application thereof, and also provides a vector containing the gene, an expression cassette, a recombinant cell, a transgenic plant and a method for producing the transgenic plant. Specifically, the gene has a nucleotide sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The plant growth regulator can be used for improving the tolerance of plants to a salt stress environment, relieving the adverse effects of a high-salt environment on the germination rate and root length of the plants, reducing the water loss rate of the plants under the high-salt stress condition and reducing the reduction of the chlorophyll content.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmPP2-10 in promoting growth of plants in a low-phosphorus stress environment. Researches show that the expression of the GmPP2-10 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the over-expression of the GmPP2-10 can increase the biomass of a transgenic plant and promote the growth of the plant under the low phosphorus condition; meanwhile, overexpression of GmPP2-10 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GmPP2-10 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved by transferring the GmPP2-10 into the plant through a transgenic technology.

The invention relates to the technical field of plant genetic engineering, and particularly provides a rice endosperm tissue specific promoter pPROLM26 and application thereof. According to the invention, a tissue-specific promoter with a nucleotide sequence as shown in SEQ ID NO.1 is separated from a rice alcohol-soluble protein gene, the tissue-specific promoter is cut off for four times, and then the cut-off promoter fragment with an endosperm-specific promoter function as shown in SEQ ID NO.2-5 is obtained after molecular identification is carried out on the cut-off promoter fragment and an RUBY visual marker; the betaine content of the expression product can reach 2.5% of the dry weight of the seeds through determination, and the method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter or the truncated promoter fragment in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

The invention belongs to the field of gene engineering, and relates to application of a soybean saline-alkaline tolerant gene GmANN13. The soybean annexin gene GmANN13 is applied to gene engineering for improving the saline-alkaline tolerance of soybeans. The invention discloses application of the soybean annexin gene GmANN13 in cultivation of saline-alkaline tolerant soybean varieties. The invention also discloses application of an expression vector containing the soybean annexin gene GmANN13 in cultivation of saline-alkaline tolerant soybean varieties. Stable transformation and saline-alkaline tolerance researches of soybean hairy roots, arabidopsis thaliana and soybeans show that the soybean GmANN13 responds to induction of saline-alkaline stress, and the saline-alkaline tolerance of plants can be improved by overexpressing the gene in the soybeans. Therefore, when the plant saline-alkaline tolerance related protein coding gene GmANN13 is transferred into crops through a genetic engineering means, a new variety of transgenic plants with saline-alkaline stress tolerance can be obtained.

The invention relates to the technical field of gene engineering, in particular to a cucumber salt-tolerant gene CsWRKY2 and application thereof. The invention provides application of a cucumber gene CsWRKY2 as shown in SEQ ID NO.1 in improvement of salt tolerance and stress resistance of plants or cultivation of salt-tolerant transgenic plants. The application comprises the following steps: constructing a recombinant expression vector containing the cucumber gene CsWRKY2 as shown in SEQ ID NO.1, transforming the recombinant expression vector into agrobacterium tumefaciens, and culturing to obtain recombinant bacteria carrying the recombinant expression vector, infecting the plant by using the recombinant bacteria carrying the recombinant expression vector; the cucumber gene CsWRKY2 is constructed into arabidopsis thaliana, so that the salt resistance of transgenic arabidopsis thaliana can be improved, and the salt resistance of cucumber hairy roots can also be improved by infecting the cucumber hairy roots with the cucumber gene CsWRKY2. And a basis is provided for breeding new stress-resistant varieties of arabidopsis thaliana and cucumbers.

Disclosed herein is a method for producing a lipid or oil in a plant, the method comprising genetically modifying the plant to express a plurality of heterologous proteins selected from PaWRI1, PaWRI2, PaDGAT1, or PaPDAT1, or variants thereof. The expression of the plurality of heterologous proteins in the genetically modified plant may result in a change in the nutrient profile of the plant relative to non-genetically modified plants of the same species. Also disclosed herein is a method for producing fatty acid and triacylglycerol content in plant non-seed tissue. Further disclosed herein is a method for producing a genetically modified plant.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

The invention relates to the technical field of functional gene and protein engineering, and discloses an amino acid sequence, a gene sequence, an expression vector and application of medicago ruthenica MrAGL8 protein, the amino acid sequence of the medicago ruthenica MrAGL8 protein is as shown in SEQ ID NO.1, the gene sequence for coding the medicago ruthenica MrAGL8 protein is as shown in SEQ ID NO.2, and the gene sequence for coding the medicago ruthenica MrAGL8 protein is as shown in SEQ ID NO.1. The invention further discloses application of the medicago ruthenica MrAGL8 protein to construction of an early-maturing plant strain or a low-pod-cracking-rate plant strain. Experiments show that the MrAGL8 gene plays an important role in promoting the early maturing of plants and reducing the pod cracking rate, and by utilizing the phenomenon that the found MrAGL8 gene participates in the response reaction of plant growth and development and pod cracking, the MrAGL8 gene is used for constructing an early maturing plant strain or a low pod cracking rate plant strain and carrying out heterologous overexpression; therefore, early maturing of transgenic plants is effectively promoted, and the pod cracking rate is remarkably reduced.

Methods and materials for modulating biomass levels in plants are disclosed. For example, nucleic acids encoding biomass-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased biomass levels and plant products produced from plants having increased biomass levels.

The invention relates to the technical field of biology, and discloses a Pm52 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof. The Pm52 protein disclosed by the invention is a protein as shown in SEQ ID No.2 in a sequence table. Pm52 protein related biological materials also belong to the protection range of the invention. The invention further discloses application of the Pm52 protein and related biological materials thereof, and the Pm52 protein and the related biological materials are used for improving the powdery mildew resistance of plants. The invention also protects a method for cultivating powdery mildew resistant wheat, which comprises the following step: introducing the nucleic acid molecule into a receptor plant to obtain a transgenic plant with enhanced powdery mildew resistance. The invention has great application and popularization value for breeding of powdery mildew of plants.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT3 is found for the first time, and the gene is obviously positively correlated with the accumulation of leucine (Leu) of crops. The invention provides an important target gene HvBCAT3 for crop quality improvement, and has important value for increasing the content of branched chain amino acids of crops and improving dietary nutrition. The HvBCAT3 gene as well as a recombinant vector, recombinant bacteria and transgenic plants thereof have good application prospects in the fields of food and feed industry.

The invention belongs to the field of biology, and particularly relates to a rhododendron molle heat shock protein RmHSP20-4 gene and application thereof. The RmHSP20-4 participates in response of rhododendron molle to high-temperature stress, and can be used for screening high-temperature tolerance germplasm of rhododendron molle and evaluating high-temperature tolerance of sheep. The gene is transferred into a prokaryotic expression strain, the growth of the transgenic strain under the stress of CdCl2 and high temperature is obviously improved, and the RmHSP20-4 gene and a protein coded by the RmHSP20-4 gene provide candidate genes for the cultivation of Rhododendron molle heavy metal resistance, high temperature stress resistance and other related varieties. A new candidate gene resource is provided for cultivating transgenic plants or microorganisms with excellent shapes such as heavy metal resistance and high temperature stress resistance, and an important reference is also provided for mining heat-resistant genes of azaleaceae plants.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention belongs to the technical field of gene engineering, and particularly relates to a highland barley flavonoid glycosyl transferase gene and application thereof. A novel glycosyl transferase gene HOVUSG6091200 in highland barley is found, and protein expressed by the gene can efficiently catalyze glycosylation modification of various flavonoid compounds, so that flavonoid compound glucoside is prepared, and the health-care value of the highland barley is improved; the gene is also transferred into tobacco, so that the highland barley flavonoid glycosyl transferase gene is also expressed in a tobacco plant, flavonoid glucoside is further generated, and the value of the tobacco plant is improved; the novel gene, the recombinant vector, the recombinant bacteria and the transgenic plant provided by the invention have good application prospects.

Methods and materials for modulating biomass levels in plants are disclosed. For example, nucleic acids encoding biomass-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased biomass levels and plant products produced from plants having increased biomass levels.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

The invention relates to the technical field of plant genetic engineering, and discloses a plant low-phosphorus-resistant important gene GsMYB7 and application thereof. According to the invention, an MYB family GsMYB7 gene is cloned in soybean for the first time. Researches show that the expression of the GsMYB7 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the overexpression GsMYB7 can obviously increase the biomass of transgenic plants and promote the growth of the plants under the low phosphorus condition; meanwhile, overexpression of GsMYB7 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GsMYB7 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved through a transgenic technology.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT1 is found for the first time, and the gene has obvious positive correlation with valine (Val) and isoleucine (Ile) accumulation of crops. The invention provides an important target gene HvBCAT1 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT1 gene as well as a recombinant vector, recombinant bacteria and transgenic plants thereof have good application prospects in the fields of food and feed industry.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.