366 results about "GMO Plants" patented technology

The invention discloses a soybean bidirectional promoter and application thereof, and belongs to the technical field of separation and application of bidirectional promoters. The nucleotide sequence of the soybean bidirectional promoter disclosed by the invention is as shown in SEQ ID NO. 2. Experiments prove that the soybean bidirectional promoter can bidirectionally and simultaneously drive the expression of a target gene, and by applying the soybean bidirectional promoter, plant traits can be improved, transgenic plants or new plant varieties can be cultivated, and the biological breeding process can be promoted.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention belongs to the field of gene engineering, and relates to application of a soybean saline-alkaline tolerant gene GmANN13. The soybean annexin gene GmANN13 is applied to gene engineering for improving the saline-alkaline tolerance of soybeans. The invention discloses application of the soybean annexin gene GmANN13 in cultivation of saline-alkaline tolerant soybean varieties. The invention also discloses application of an expression vector containing the soybean annexin gene GmANN13 in cultivation of saline-alkaline tolerant soybean varieties. Stable transformation and saline-alkaline tolerance researches of soybean hairy roots, arabidopsis thaliana and soybeans show that the soybean GmANN13 responds to induction of saline-alkaline stress, and the saline-alkaline tolerance of plants can be improved by overexpressing the gene in the soybeans. Therefore, when the plant saline-alkaline tolerance related protein coding gene GmANN13 is transferred into crops through a genetic engineering means, a new variety of transgenic plants with saline-alkaline stress tolerance can be obtained.

The invention relates to the technical field of gene engineering, in particular to a cucumber salt-tolerant gene CsWRKY2 and application thereof. The invention provides application of a cucumber gene CsWRKY2 as shown in SEQ ID NO.1 in improvement of salt tolerance and stress resistance of plants or cultivation of salt-tolerant transgenic plants. The application comprises the following steps: constructing a recombinant expression vector containing the cucumber gene CsWRKY2 as shown in SEQ ID NO.1, transforming the recombinant expression vector into agrobacterium tumefaciens, and culturing to obtain recombinant bacteria carrying the recombinant expression vector, infecting the plant by using the recombinant bacteria carrying the recombinant expression vector; the cucumber gene CsWRKY2 is constructed into arabidopsis thaliana, so that the salt resistance of transgenic arabidopsis thaliana can be improved, and the salt resistance of cucumber hairy roots can also be improved by infecting the cucumber hairy roots with the cucumber gene CsWRKY2. And a basis is provided for breeding new stress-resistant varieties of arabidopsis thaliana and cucumbers.

Disclosed herein is a method for producing a lipid or oil in a plant, the method comprising genetically modifying the plant to express a plurality of heterologous proteins selected from PaWRI1, PaWRI2, PaDGAT1, or PaPDAT1, or variants thereof. The expression of the plurality of heterologous proteins in the genetically modified plant may result in a change in the nutrient profile of the plant relative to non-genetically modified plants of the same species. Also disclosed herein is a method for producing fatty acid and triacylglycerol content in plant non-seed tissue. Further disclosed herein is a method for producing a genetically modified plant.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

The invention belongs to the field of biology, and particularly relates to a rhododendron molle heat shock protein RmHSP20-4 gene and application thereof. The RmHSP20-4 participates in response of rhododendron molle to high-temperature stress, and can be used for screening high-temperature tolerance germplasm of rhododendron molle and evaluating high-temperature tolerance of sheep. The gene is transferred into a prokaryotic expression strain, the growth of the transgenic strain under the stress of CdCl2 and high temperature is obviously improved, and the RmHSP20-4 gene and a protein coded by the RmHSP20-4 gene provide candidate genes for the cultivation of Rhododendron molle heavy metal resistance, high temperature stress resistance and other related varieties. A new candidate gene resource is provided for cultivating transgenic plants or microorganisms with excellent shapes such as heavy metal resistance and high temperature stress resistance, and an important reference is also provided for mining heat-resistant genes of azaleaceae plants.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

The invention belongs to the technical field of agricultural biology, and particularly relates to a plant constitutive promoter OsSULTR2. 2pro and an application thereof. The promoter is characterized in that the promoter is OsSULTR2; the nucleotide sequence of the 2pro is as shown in SEQ ID NO. 1. The promoter provided by the invention is OsSULTR2; 2pro is a rice endogenous constitutive promoter, can drive a target gene to realize efficient and stable expression in plant callus, roots, stems, leaves, young ears, seeds and other tissues in a vegetative growth period, and can replace an existing non-plant-source promoter. The method has important application value in the field of plant genetic engineering, and can effectively reduce potential safety risks of transgenic plants caused by introduction of exogenous DNA.

The present invention relates to a recombinant construct comprising: a first expression cassette comprising a fluorescence enhancing factor; wherein the amino acid sequence of the fluorescence enhancement factor is as shown in SEQ ID NO: 2, or an amino acid sequence which at least has 60%, 65%, 70%, 75%, 80%, 85% or 90% identity with the SEQ ID NO: 2 and is functionally equivalent to the protein as shown in the SEQ ID NO: 2; the first expression cassette is configured to increase the self-luminous intensity of a plant, plant tissue, or cell. According to the application, the LumEnhancer gene is further introduced on the basis of an FBP biological self-luminous system, so that the physiological activity of transgenic plant cells is effectively improved, and the energy metabolism level of the plant is further enhanced, thereby providing a more sufficient energy basis for a bioluminescence reaction and effectively enhancing the luminous intensity of the whole plant.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention relates to the technical field of plant breeding, in particular to a primer combination for detecting a regulatory element and an exogenous gene of a transgenic plant and application of the primer combination. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. 22-63. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. The application comprises: (1) transgenic plant variety germplasm supervision or transgenic plant component screening in non-transgenic varieties; (2) screening transgenic ingredients of the plant product; (3) auxiliary screening in the plant breeding process; and (4) tracing plant varieties. The invention provides a primer combination which can be applied to detection of regulatory elements and exogenous genes of transgenic plants, can realize detection of a plurality of regulatory elements and exogenous genes in a reaction system, and can meet high-throughput screening of components of the transgenic plants. The method is used for monitoring illegal planting of transgenic plants and detecting transgenic components of related plant products.

The present application relates to the field of genetic engineering, and in particular to a vector for analyzing plant promoter expression specificity, a preparation method and application thereof. The T-DNA region of the vector comprises, from 5' end to 3' end, a callus-specific promoter-driven selection marker gene expression cassette and a reporter gene expression cassette with a multiple cloning site; the callus-specific promoter-driven selection marker gene expression cassette comprises a callus-specific promoter, a selection marker gene coding sequence and a terminator. The vector of the present application utilizes a rice callus-specific promoter to drive the expression of a selection marker gene, can greatly reduce the non-specific interaction with the promoter of a target gene, improve the specificity of the expression of the target gene, and effectively reduce the biological safety risk caused by the selection marker gene in a transgenic plant, and has important application value in basic theoretical research and molecular breeding.

The present application relates to the technical field of intelligent identification system of crop pests, in particular to a plant field pest fine-grained identification method, system, device and storage medium based on deep learning. The identification method provided by the present application is specialized in high-precision identification of real field scenes, and can provide technical support for important work such as future development of field inspection robot, automatic identification and monitoring system of field pests and the like. In addition to pest monitoring, the field biological safety test of genetically modified plants is gradually carried out at present, and by using the identification method provided by the present application, the dynamic change of farmland insect community can be quickly and accurately identified and predicted, so that the efficiency and accuracy of ecological investigation are greatly improved.

The application discloses a protein separated from Pinus tabulaeformis Carr. Pinus tabuliformis ​ and an encoding gene PtAMT2.8 of the protein, which can significantly enhance nitrogen absorption and transportation of plants. 31019b The PtAMT2.8 gene is introduced into a yeast mutant strain with a deficiency in ammonium nitrogen absorption function, so that the growth phenotype of the yeast mutant is recovered, and the PtAMT2.8 gene is introduced into Pinus tabulaeformis and Arabidopsis thaliana, so that the nitrogen absorption and transportation capacity of the transgenic plants can be significantly enhanced. The application has important significance for accelerating genetic improvement of plants and cultivating new varieties with high yield, high quality and multi-resistance.

The invention discloses a protein which is separated from pinus tabuliformis and is capable of remarkably enhancing nitrogen absorption and transport of a plant, and a coding gene PtAMT2.8 of the protein. The PtAMT2.8 gene is introduced into a yeast ammonium nitrogen absorption function deficient mutant strain 31019b, so that the growth phenotype of a yeast mutant can be recovered, and the nitrogen absorption and transport capability of a transgenic plant can also be remarkably enhanced by introducing the PtAMT2.8 gene into pinus tabulaeformis and a model plant arabidopsis thaliana. The invention has important significance in accelerating plant genetic improvement and cultivating high-yield, high-quality and multi-resistant new varieties.

The invention belongs to the technical field of gene engineering, and particularly relates to a cornutgiella axataenae PdMYB44 gene and application thereof. The invention provides a cornutgiella dotacea PdMYB44 gene, wherein the CDS (Coding Sequence) of the PdMYB44 gene is as shown in SEQ ID NO. 1. The PdMYB44 gene disclosed by the invention is derived from the cornutgiium axataenense, so that the drought resistance of a transgenic plant can be improved, the flowering time is shortened, and the PdMYB44 gene can be applied to plant stress resistance genetic improvement. Results of embodiments show that according to a transgenic plant over-expressing the PdMYB44 gene, the proline content is increased, the malondialdehyde content is reduced, the transpiration rate and stomatal conductance are remarkably reduced, and the drought resistance of the transgenic plant is jointly improved by synergistically regulating three key mechanisms, namely accumulation of permeation protection substances, inhibition of membrane damage and optimization of stomatal behaviors.

The present invention relates to a method for producing diosmin in plants by reconstructing the diosmin biosynthetic pathway. Reconstructing the diosmin biosynthetic pathway by selecting optimal genes according to the present invention has the effect of enabling mass production of diosmin or a precursor thereof in plants, and therefore can be advantageously used for large-scale production of diosmin or a precursor thereof in the form of a gene expression cassette, a recombinant vector, a transformant, a transgenic plant, a method for producing a transgenic plant, or a method for producing diosmin or a precursor thereof.

The invention provides an application of ACR11 protein in improving the content of plant protein and the utilization efficiency of urea, and provides an application of the ACR11 protein in improving the yield of the plant protein and / or improving the utilization efficiency of the plant to a urea nitrogen fertilizer, and the ACR11 protein can improve the yield of the plant protein and improve the utilization efficiency of the plant to the urea nitrogen fertilizer. The ACR11 protein disclosed by the invention can also improve the utilization efficiency of a plant on urea nitrogen fertilizer, and is specifically characterized in that a transgenic plant formed by transferring the coding gene of the duckweed ACR11 protein disclosed by the invention into a receptor plant is cultured under the condition of taking urea as a nitrogen source; compared with a receptor plant cultured under the same condition, the transgenic plant has higher biomass, higher protein content, higher protein yield and higher protein production efficiency under the same urea nitrogen fertilizer input. The invention provides a new way for improving the utilization efficiency of urea by crops and improving the protein yield of the crops in agriculture, and is beneficial to promoting the sustainable development of green agriculture.

The invention discloses a blackberry RubHLH1 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the blackberry RubHLH1 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of expressed protein is as shown in SEQ ID NO. 2. The RubHLH1 gene is genetically transformed into arabidopsis thaliana, and a RubHLH transgenic arabidopsis thaliana plant shows a plurality of beneficial characters compared with a wild type plant at the same growth time, including that the stem length is fast and thick, the SPAD value is high, and podding is carried out in advance; wherein stem segments and petiole epithelium spines of the bHLH-3 strain are more than those of a wild type, the leaf surface is more shrunk, and the number of pores is large. The activities of POD, SOD and CAT and the contents of MDA, H2O2, total phenols and flavonoids in a transgenic plant are higher than those in a CK contrast. The invention provides an efficient gene resource and a molecular tool for cultivating excellent transgenic plant varieties by using a molecular breeding means.

The present disclosure provides a transgenic plant comprising one or more nucleotide sequences encoding polypeptides selected from photosystem II subunit S (PsbS), zeaxanthin epoxidase (ZEP), and violaxanthin de-epoxidase (VDE), operably linked to at least one expression control sequence. Expression vectors for making transgenic plants, and methods for increasing biomass production and / or carbon fixation and / or growth in a plant comprising increasing expression of at least one of PsbS, ZEP and VDE polypeptides are also provided.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The invention relates to the field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The present invention provides a nucleic acid molecule having a nucleotide sequence as any of SEQ ID NO: 1 to SEQ ID NO: 7. The invention provides a left flanking sequence and a right flanking sequence of an exogenous insertion vector of an insect-resistant and herbicide-resistant corn WYN8158 transformation event and application of the left flanking sequence and the right flanking sequence in detection of transgenic corn, and can be used for rapidly, efficiently and specifically detecting whether a sample to be detected is derived from a transgenic component or not. Meanwhile, technical support is provided for market identification and identification after commercialization and industrialization of the product, and meanwhile the method has important significance and wide prospects for promoting corn germplasm resource identification in China.

The invention belongs to the field of gene engineering, and relates to a chalkiness-related protein OsGAPDH of rice endosperm as well as a coding gene and application of the chalkiness-related protein OsGAPDH. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO: 3 in a sequence table; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO: 3, is related to plant starch synthesis and is derived from SEQ ID NO: 3. The protein provided by the invention affects normal development of rice endosperm. The coding gene of the protein is introduced into Ningjing No.7 wild type, so that a transgenic plant of which the chalkiness of endosperm is obviously reduced can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The present invention relates to a method for preparing a MAGEA8-Fc transgenic plant. Through a platform using a tobacco plant, a recombinant protein MAGEA8-Fc, in which a human IgG Fc fragment is fused to a MAGEA8 protein, can be stably expressed. The method of the present invention enables high-level expression efficiency and stable protein production by introducing a target gene into a plant genome using an Agrobacterium binary vector. By directly isolating and purifying the recombinant protein from the transgenic tobacco plant, large-scale production can be easily achieved. Compared to conventional microbial or animal cell-based expression systems, the method provides economic efficiency and scalability, and thus can be usefully applied as a platform for producing fusion proteins.