90 results about "Herbicide resistant" patented technology

The present application relates to a kind of deletion mutant nucleic acid and its application in anti-herbicide.The deletion mutation occurs in the promoter of rice OsHPPD Gene, the length of deletion sequence is at least 10 bp, and the A in the start codon ATG of gene is 0, at least the bases located in the upstream of start codon ATG from 2041 to 2032 are deleted. OsHPPD The present application finds that by deleting part of the sequence in the promoter of rice OsHPPD Gene, rice can obtain resistance to HPPD inhibitor herbicides, which is of great application value for ensuring agricultural production safety and improving the efficiency of herbicide use.

The invention relates to a deleted mutant nucleic acid and application thereof in herbicide resistance. The deletion mutation occurs in a promoter of a rice OsHPPD gene, the length of a sequence of a deleted nucleic acid is at least 25 bp, A in an initiation codon ATG of the OsHPPD gene is taken as a 0 site, and at least bases at 2299 to 2275 sites on the upstream of the initiation codon ATG are deleted. According to the application disclosed by the invention, the rice can obtain resistance to HPPD inhibitor herbicides by deleting part of sequences in the promoter of the rice OsHPPD gene, so that the application has great application values in guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

The invention relates to a deleted mutant nucleic acid and application thereof in herbicide resistance. The deletion mutation occurs in a promoter of the rice OsHPPD gene, the length of a deletion sequence is at least 10 bp, A in an initiation codon ATG of the OsHPPD gene is taken as a 0 site, and at least 2041-2032 sites of basic groups located on the upstream of the initiation codon ATG are deleted. According to the application disclosed by the invention, the rice can obtain resistance to HPPD inhibitor herbicides by deleting part of sequences in the promoter of the rice OsHPPD gene, so that the application has great application values in guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

The invention relates to the technical field of gene editing, and particularly provides a method for creating herbicide-resistant rice through gene editing of an OsEPSPS gene promoter region, and the method is characterized in that WT / 29bp single allelic deletion mutation is introduced into the OsEPSPS gene promoter region, i.e., A in an OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, and A in the OsEPSPS gene initiation codon ATG is 0 site; 29bp is deleted from the 112nd site to the 1150th site at the upstream of the initiation codon ATG on one allele, and the other allele is kept unchanged. By introducing the single allelic deletion mutation, the rice can obtain resistance to EPSPS inhibitor herbicides, the resistance is identified in T0-generation plants carrying the mutation and can be stably inherited to offspring plants, and the application value is high for guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

The invention belongs to the technical field of herbicide resistance genes, and relates to a rice herbicide resistance gene and application thereof. The herbicide-resistant gene provided by the invention is OsCYP704A5, and the sequence of the herbicide-resistant gene is as shown in SEQ ID NO. 1. According to the application disclosed by the invention, molecular docking analysis is carried out on the OsCYP704A5 gene and the triazole sulcotrione, and the result shows that the OsCYP704A5 and the triazole sulcotrione have relatively high combining capacity and have a certain promoting effect on detoxification metabolism. The OsCYP704A5 gene is further over-expressed in Nipponbare rice, so that the transgenic rice can obtain the drug resistance to triazole sulcotrione. The HPPD inhibitor herbicide-resistant rice strain can be used for cultivating HPPD inhibitor herbicide-resistant rice varieties, and provides a new possibility for researching HPPD inhibitor herbicide resistance of paddy field weeds (barnyard grass).

The invention relates to the field of plant genetic engineering. Specifically, the invention relates to a method for creating a novel herbicide-resistant plant through a plant basic group editing technology and screening an endogenous gene mutation site capable of endowing the plant with herbicide resistance. The invention also relates to the use of the identified mutated endogenous resistance gene in crop breeding.

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

The invention discloses a corn ALS mutant gene and application of the corn ALS mutant gene in herbicide resistance. The invention provides a method for improving the herbicide resistance of corn, which comprises the following steps: mutating 493th nucleotides of a ZmALS1 gene and a ZmALS2 gene in a corn genome from C to G so as to improve the herbicide resistance of the corn, the nucleotide sequence of the ZmALS1 gene is shown in SEQ ID No. 1; the nucleotide sequence of the ZmALS2 gene is shown in SEQ ID No. 2. Specific sites in corn ZmALS1 and ZmALS2 serve as sgRNA recognition sites on the basis of the principle of a base editing technology, a single base replacement editing vector capable of simultaneously targeting the ZmALS1 and ZmALS2 is designed, the gene editing vector is stably transformed through agrobacterium to obtain a transgenic plant, and the resistance to herbicides is remarkably improved.

The present invention relates to the fields of agriculture, plant biotechnology, and molecular biology. Specifically, the present invention relates to a wheat EPSPS mutant protein and use thereof in improving the tolerance of wheat to glyphosate. The mutant protein comprises an amino acid sequence having the following mutations compared to the EPSPS amino acid sequence of any genome of wild-type wheat: in the amino acid sequence set forth in SEQ ID NO: 2, the amino acid at position 168 is mutated from threonine to isoleucine, the amino acid at position 169 is mutated from alanine to valine, and the amino acid at position 172 is mutated from proline to serine. The mutant protein enables the production of stable glyphosate-resistant wheat, showing important significance for wheat breeding.

The application discloses a P450 enzyme mutant for degrading herbicides and application thereof, and the P450 enzyme mutant is named P450 BSβ -F46A, P450 BSβ -F46A-CPR; the amino acid sequences are shown in SEQ ID NO. 2 and 4 respectively. The application also discloses application of the P450 enzyme mutant in degrading pyridine oxycarboxylic acid herbicides or aryloxyphenoxypropionic acid herbicides and related genes in cultivating herbicide-resistant transgenic crops. Experiments prove that the highest conversion rate of the P450 enzyme mutant for degrading pyridine oxycarboxylic acid herbicides reaches 86%, and the total conversion number of catalyzing flumiclorac can reach 7443 at most, and the highest catalytic conversion rate of degrading aryloxyphenoxypropionic acid herbicides reaches 89%. The P450 enzyme mutant provided by the application has the advantages of high catalytic efficiency, high protein expression amount and low industrial cost, and the whole operation process for degrading herbicides is simple, the process is mature, the cost is low, and the application prospect is wide.

The application discloses a sulfonylurea herbicide hydrolytic enzyme TsmE mutant, a coding gene thereof and a use thereof. The amino acid sequence of the mutant is shown in SEQ ID NO. 4. The mutant M6 provided by the application has deesterification activity to macromolecular sulfonylurea herbicides methyldisulfuron and sodium salt of methyl iodosulfuron, and therefore has a good application prospect in sulfonylurea herbicide residual pollution bioremediation and anti-herbicide transgenic engineering.

The present invention provides a mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid and an application thereof, and in particular, relates to a mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid and an application thereof in plant breeding. In particular, the present invention provides a mutant acetyl-CoA carboxylase (ACC) protein, and the mutant acetyl-CoA carboxylase (ACC) protein is compared with the parent acetyl-CoA carboxylase (ACC) protein in the region corresponding to SEQ ID Mutations occur at any one or several of the following amino acid positions in the amino acid sequence shown in No. 1: 2125, 2097, 2139, 2194, 2186, 2273, 2168, 1975, 1954, 1864, 2211, 2187, 2123, and 2126; plants with the acetyl-CoA carboxylase (ACC) mutation have high herbicide resistance and have very broad application prospects in breeding herbicide-resistant plants.

This invention relates to the field of gene editing technology, specifically providing a method for creating herbicide-resistant rice by gene editing the promoter region of the OsEPSPS gene. The method involves introducing a WT / -29bp monoallelic deletion mutation into the OsEPSPS gene promoter region. Specifically, with position A of the OsEPSPS gene start codon ATG set to 0, a 29bp deletion is made in one allele located at positions 1122 to 1150 upstream of the start codon ATG, while the other allele remains unchanged. This invention demonstrates that by introducing this monoallelic deletion mutation, rice can acquire resistance to EPSPS inhibitor herbicides. This resistance was identified in the T0 generation plants carrying this mutation and can be stably inherited by their offspring, possessing significant application value for ensuring agricultural production safety and improving herbicide application efficiency.

The invention provides a novel herbicide-resistant mutation efficiently created by editing a rice genome targeted G-to-T basic group. Specifically, the invention provides a separated herbicide resistant polypeptide, and the herbicide resistant polypeptide is a mutant ALS1 polypeptide, the mutant ALS1 polypeptide is mutated at the 171st amino acid, corresponding to SEQ ID NO.1, of the wild ALS1 polypeptide, and proline (P) at the 171st position is mutated into one or more amino acids selected from the following groups: threonine (T), histidine (H), asparagine (N) and arginine (R). According to the invention, it is found for the first time that a guanine base editor is used for editing the wild ALS1 polypeptide in a plant; efficient G-to-T base editing can be achieved, multiple amino acid replacement types which are not reported before are created, and partial mutation types can greatly improve the tolerance of plant herbicides.

The present application relates to a herbicide-resistant HPPD mutant protein. At least three excellent mutants are obtained by gene editing technology, which express different HPPD mutant proteins and can confer herbicide resistance or tolerance to plants.

The invention discloses an active polypeptide of protoporphyrinogen oxidase and application, the polypeptide has at least 85%, 90%, 95%, 99% and 100% sequence identity of an amino acid sequence as shown in one of SEQ ID NO: 1-42, and has protoporphyrinogen oxidase activity. The active polypeptide disclosed by the invention shows relatively high tolerance to PPO inhibitor herbicides, and has a wide application prospect in plants. According to the weed control method provided by the invention, the occurrence of resistant weeds can be effectively reduced, and transgenic crops obtained by the method can resist various herbicides such as PPO inhibitors, ACC enzyme inhibitors, ALS inhibitors, EPSPS inhibitors and synthetic auxin at the same time, and have broad-spectrum herbicide resistance; in the weed control process, the PPO inhibitor herbicide is used in combination with one or more other herbicides, so that the cost can be effectively reduced, the control efficiency can be improved, and the weed resistance can be greatly avoided.

Screening assays are used to identify mutant homogentisate solanesyl transferase (HST) enzymes which are at least partially resistant to HST-inhibiting herbicides. Nucleic acids encoding the mutant HST enzyme proteins are made available and useful in modifying plants and plant parts so that they can be made herbicide resistant. Crop plants which express the mutant HST enzymes can be grown in the field and herbicides used to controlling unwanted other vegetation. The homogentisate solanesyltransferase (HST) enzyme or an active fragment thereof comprises the amino acid sequence motif: F[V / M]TX[F / Y] (SEQ ID NO: 1), wherein X is any amino acid; and wherein one or more of the amino acid residues of the motif are mutated.

This invention discloses a breeding method for improving japonica rice by cultivating polymorphic lines. This invention integrates the GS3 long-grain gene, PigmR disease-resistant gene, fgr aroma gene, ALS herbicide-resistant gene, and COLD1 cold-tolerant gene into japonica rice. The GS3 long-grain trait successfully compensates for the reduction in thousand-grain weight caused by the PigmR gene. Simultaneously, the COLD1 cold-tolerant genotype repairs the genetic defect of low-temperature intolerance in the GS3 donor parent. Furthermore, it demonstrates the heat-resistant function of GS3 in the context of polymorphic lines, simultaneously improving grain shape, blast resistance, aroma, herbicide resistance, heat tolerance, and low-temperature tolerance. This provides new germplasm for the green and efficient production of japonica rice and has significant application value.

The invention provides a novel nucleic acid molecule and application thereof in cultivating herbicide-resistant plants. The nucleic acid molecule provided by the invention can improve the tolerance of corn to quizalofop-p-ethyl and 2, 4-D herbicides, can be used for cultivating novel herbicide-resistant crops, and provides a solution for controlling the growth of weeds in agricultural production.

The invention discloses a non-transgenic herbicide-resistant Chinese cabbage or Chinese cabbage type rape strain as well as a cultivation method and application thereof, and aims to solve the problem of weeds in the production practice of Chinese cabbage or Chinese cabbage type rape, a non-transgenic herbicide-resistant gene of a sibling species is transferred into Chinese cabbage or Chinese cabbage type rape through a distant hybridization technology; the non-transgenic herbicide-resistant Chinese cabbage or Chinese cabbage type rape germplasm is obtained; the non-transgenic herbicide-resistant Chinese cabbage or Chinese cabbage type rape germplasm is used as a herbicide-resistant character donor, and is hybridized with other varieties to obtain a new variety of Chinese cabbage or Chinese cabbage type rape with resistance to specific herbicides through screening. After the new non-transgenic herbicide-resistant cabbage or cabbage type rape variety is applied to production, the herbicide can be sprayed in the early stage, the growth of the cabbage or cabbage type rape is not affected while weed seedlings are killed in the early stage, the weed problem is effectively solved, and cost saving and efficiency increasing in the weeding link are achieved.

The invention relates to the technical field of gene editing, and particularly provides rice OsEPSPS gene promoter region deletion mutation and application thereof in creation of herbicide-resistant rice, the deletion mutation is that a sequence with the length of 22bp or 28bp is deleted in the OsEPSPS gene promoter region, namely, A in an OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, and A in the OsEPSPS gene initiation codon ATG is 0 site; and deleting the basic groups at sites 2534 to 2555 upstream of the initiation codon ATG or the basic groups at sites 2527 to 2554 upstream of the initiation codon ATG. According to the application disclosed by the invention, the rice can obtain resistance to EPSPS inhibitor herbicides by deleting part of sequences in the promoter of the rice OsEPSPS gene, so that the application has great application values in guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

Provided is a method for effectively controlling specific weeds having resistance to herbicides. The method comprises applying one or more uracil compounds selected from a group consisting of a compound represented by formula (I):and a compound represented by formula (II):to a PPO inhibitor-resistant weed which has one or more mutations selected from a group consisting of Arg128Met mutation, Arg128Gly mutation, Arg128His mutation, Arg128Ile mutation, Arg128Lys mutation, and Gly399Ala mutation in PPO.

The invention relates to the technical field of biology, and particularly discloses a rice ALS mutant protein ALS-ANTSL and application of the rice ALS mutant protein ALS-ANTSL. Compared with a wild type rice ALS protein, the rice ALS mutant protein disclosed by the invention contains mutation that valine at the 170th position is mutated into alanine, proline at the 171st position is mutated into asparagine, arginine at the 172nd position is mutated into threonine, arginine at the 173rd position is mutated into serine, and methionine at the 174th position is mutated into leucine. According to the rice ALS mutant protein disclosed by the invention, various plants can have relatively high resistance to various ALS inhibitor herbicides, and the rice ALS mutant protein can be used for cultivating herbicide-resistant plant varieties and has relatively high application value.

The invention relates to a rice OsACC gene promoter mutant for resisting ACCase inhibitor herbicides and application of the rice OsACC gene promoter mutant. The rice OsACC gene mutant is a deletion mutant or a deletion and combination insertion mutant; the deletion mutation occurs in the promoter of the rice OsACC gene, and the sequence of the nucleic acid before deletion mutation is as shown in SEQ ID No. 1; the length of the sequence of the deleted nucleic acid is 29 bp to 97 bp, A in the initiation codon ATG of the OsACC gene is 0 site, the deleted nucleic acid occurs in the 305-209 sites of the upstream of the initiation codon ATG, and at least the 271-243 sites of the upstream of the initiation codon ATG are deleted; the inserted nucleic acid occurs within sites 271 to 243 upstream of the initiation codon ATG. The mutant can enable rice to obtain resistance to at least one of herbicides such as haloxyfop-R-methyl, fluazifop-p-butyl and clethodim.

The invention provides development and application of an anti-pyrithiobac-sodium KASP molecular marker, and belongs to the technical field of genetic typing. The invention provides a KASP typing primer group for detecting the SNP site of the millet anti-pyrithiobac-sodium gene, and verifies that the resistance of millet to pyrithiobac-sodium herbicide is caused by single SNP mutation of an ALS target site. The KASP primers are designed on the basis of SNP loci, the primer pair can be used for accurately distinguishing resistance, susceptibility and heterozygous genotypes, and the method has the advantages of being stable and reliable in result, easy and convenient to operate, rapid and high in automation degree. By adopting the molecular marker to assist breeding, the breeding of the pyrithiobac-sodium-resistant millet matching variety can be accelerated, the breeding period can be shortened, the breeding efficiency can be improved, and the high-throughput identification of the pyrithiobac-sodium-resistant herbicide genotype and the molecular marker to assist the breeding of herbicide-resistant millet are significant.

This invention discloses a herbicide-resistant cytochrome P450 gene, BjCYP71C2, its protein, plasmid, and their applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene is SEQ ID NO:1 or has at least 80% identity with SEQ ID NO:1. This invention also discloses the protein polypeptide and plasmid encoded by the above gene. The purpose of this gene is to express it transgenic in plants, cultivating herbicide-resistant plants for selective weed control using herbicides.

The present invention relates to modified protoporphyrinogen oxidase (PPO) or functional fragments thereof having increased resistance to PPO inhibiting compounds, plants or parts thereof comprising these modified PPO enzymes, uses thereof and methods of using these plants or parts thereof to control undesirable vegetation.

A method for controlling PPO resistant weeds, wherein compounds of formula (I) wherein the variables are defined as given in the description and claims; are applied to the PPO inhibitor herbicide resistant weed, parts of it or its propagation material.

The invention discloses a EcCYP81A105 gene and application thereof, and belongs to the technical field of plant biology, the nucleotide sequence of the EcCYP81A105 gene is shown as SEQ ID NO.1, and the amino acid sequence of the EcCYP81A105 gene is shown as SEQ ID NO.2. The invention finds that the EcCYP81A105 gene has the function of improving the drug resistance of rice to ACCase herbicides for the first time, and can be used as a resistance gene for cultivating herbicide-resistant rice germplasm.

The invention discloses a herbicide-resistant gene StGSTF8 in sagittaria trifolia and application of the herbicide-resistant gene StGSTF8, and belongs to the technical field of genetic engineering. The bensulfuron methyl resistance gene StGSTF8 (glutathione-S-transferase F8) is identified from sagittaria trifolia for the first time by means of proteomics, molecular biology, transgenic function verification and the like, the nucleotide sequence is as shown in SEQ ID NO: 1, the coded amino acid sequence is as shown in SEQ ID NO: 2, and the nucleotide sequence is as shown in SEQ ID NO: 1. It is proved that the strain endows plants with resistance through mediating herbicide detoxification metabolism and oxidative stress resistance, and reference is provided for improvement of the resistance of plants to sulfonylurea herbicides and research and development of novel herbicides.