300 results about "Herbicide resistant" patented technology

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide.

The invention provides cropping systems for managing weeds in crop environments. The cropping systems comprise, in one embodiment, transgenic plants that display tolerance to an auxin-like herbicide such as dicamba. Method for minimizing the development of herbicide resistant weeds are also provided.

Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of the Brassica juncea B genome. Two B. juncea AHAS gene sequences (BjAHAS-a and BjAHAS-b) and one B. nigra AHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutant B. juncea lines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.

The present invention provides compositions and crystals of the carboxyltransferase (CT) domain (the C-terminal ˜90 kDa fragment) of various acetyl-CoA carboxylase (ACC) proteins, including yeast, mouse and human ACCs. Further, the present invention provides methods for identifying and designing compounds that can modulate ACC activity. These methods are based, in part, on the X-ray crystallographic structures of the CT domain of yeast ACC, either alone or bound to acetyl-CoA or a CT inhibitor, such as haloxyfop or diclofop or CP-640186. Thus, the present invention relates to the crystal structures of the carboxyltransferase (“CT”) domain of acetyl-CoA carboxylase (“ACC”), and to the use of these structures in the design of anti-obesity compounds, anti-diabetes compounds, antibiotic compounds, herbicide compounds, and in the design of herbicide resistant plants.

Described are polynucleotides having nucleotide sequences encoding mutant plant phytoene desaturase proteins that are resistant the bleaching herbicides that act on phytoene desaturase, and related nucleic acid constructs, plants and methods.

The invention belongs to the technical field of bioengineering and in particular relates to a gene site-directed mutation vector as well as a construction method and application thereof. The invention provides the gene site-directed mutation vector due to lots of optimizations, cytosine deaminase is guided to be close to cytosine through a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, the cytosine is changed into uracil, then uracil is changed into thymine through self-repair in plant cells, and finally, site-specific mutagenesis from C to T is realized in plants so as to generate resistance to herbicides. In addition, point mutation can be produced in a non-sgRNA targeted area, and novel herbicide-resistant great agronomic traits are brought.

Canola plants with high oleic acid and low linolenic acid content are disclosed. In addition, the canola plants of the invention are resistant to imidazolinone herbicides and are resistant to blackleg. The canola plants of the invention also have low total glucosinolate content. There is also provided a method for controlling weeds in a field of canola plants wherein the canola plants are herbicide resistant.

The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a molecular marker in close linkage with a foxtail millet herbicide resistant gene, with a nucleotide sequence of the molecular marker shown as SEQID NO:1, or a DNA fragment containing the nucleotide sequence shown in the SEQ ID NO:1 in a foxtail millet genome. The invention also relates to primers of the molecular marker, application of the molecular marker in the positioning of the foxtail millet herbicide resistant gene or in foxtail millet inheritance breeding, a foxtail millet herbicide resistant gene positioning method, and a foxtail millet breeding method. The invention finds the molecular marker SIsv0372 which is in close linkage with the foxtail millet herbicide resistant gene, associates the foxtail millet genome DNA sequence and the foxtail millet herbicide resistant gene, and better benefits the establishment of a foxtail millet molecular marker assisted breeding system.

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene. The amino acids at the positions 126, 177, 207, 438, 479, 480 and/or 505 are changed tp produce a mutant EPSPS gene product.

A protoporphyrinogen oxidase tolerant to photobleaching herbicide and derivatives thereof, comprising a polypeptide having the amino acid sequence represented by SEQ ID No. 2 or mutated peptides derived therefrom by deletion, addition, substitution, etc. of one or more amino acids in the above amino acid sequence and having an activity substantially equivalent to that of the protoporphyrinogen oxidase. The acquisition of the novel protoporphyrinogen oxidase, which is highly tolerant to photobleaching herbicide and originates in plant, make it possible to construct plants highly tolerant to photobleaching herbicide via the expression of this enzyme in host plants.

The present invention provides a compound represented by the formula:

wherein Q represents a fused heterocyclic group, X and Y are the same or different and each represent an optionally halogenated lower alkyl group, an optionally halogenated lower alkoxy group, etc., or a salt thereof, as well as a herbicide comprising the compound or a salt thereof, which exhibits a significant effect for control of sulfonylurea herbicide-resistant weeds in paddy fields and can reduce the number of active ingredients in a combined preparation and a method of controlling sulfonylurea herbicide-resistant weeds which comprises using the same.

The invention provides a preparation method of glyphosate resistant transgenic rice. The method comprises the following steps: converting an expression vector containing glyphosate resistant gene into Agrobacterium tumefaciens, invading rice calluses by the Agrobacterium tumefaciens, transferring the calluses to a selective aluminum containing glyphosate for screening, choosing resistant calluses, differentiating, rooting, hardening-seedling, and transplanting to obtain glyphosate resistant transgenic rice. A molecular identification result shows that an exogenous gene is stably expressed. The obtained glyphosate resistant transgenic rice contains glyphosate herbicide resistant gene, contains no other screening marker genes, and can be used as a commercial herbicide resistant rice kind to reduce the reduce the later process and accelerate the breeding pace. The method has the advantages of simple operation, low copy number of transgenic plants, stable heredity properties, and good market application prospect.

The invention provides a herbicide-resistant rape directive breeding method based on acetolactate synthase (ALS) target esterase, which belongs to a plant trait breeding method. The method comprises the following steps of selecting materials needed by production of rape or breeding of the rape, processing seeds with ethylmethane sulfonate (EMS), mutagenizing the seeds to be isolated and propagated to a M2 generation, and directionally screening herbicide-resistant mutant characters in a large group under the selection pressure of the herbicide adopting the ALS as the target esterase. In order to increase the probability for acquiring the mutant characters, the directive screening can be continuously conducted in multiple years, or conducted in multiple places in the same year or conducted in multiple places in multiple years until the needed mutant material is screened out.

Herbicidal compositions are disclosed comprising an effective amount of flufenacet, diflufenican and metribuzin. A method of controlling harmful plants is disclosed. The compositions may be used flexibly, e.g, in post-emergent and/or pre-emergent applications. The compositions may be used prior to sowing a crop. The compositions may provide enhanced compatibility with crops. The compositions may be used on different soil types, e.g., with high-organic matter soils and/or at different soil depths, e.g., at shallow soil depths. The compositions may be used against herbicide-resistant plant species, for example EMR and TSR-resistant species and/or may be used for resistance management. The compositions may be used with various irrigation techniques.

The invention discloses a herbicide-resistant gene, wherein a nucleotide sequence of the gene is SEQ ID NO: 1 or has more than 90 percent of identity with the SEQ ID NO: 1. The invention also discloses herbicide-resistant protein encoded by the herbicide-resistant gene, wherein an amino acid sequence of the protein is SEQ ID NO: 2 or has more than 90 percent of identity with the SEQ ID NO: 2. The gene can be used for preparing a transgenic plant which increases the resistance to a herbicide, or obtaining a method which utilizes the herbicide to selectively kill transgenic corn.

The invention discloses a rice ACCase mutant gene and an application thereof in herbicide resistance of plants. The amino acid sequence of the encoding protein of the ACCase mutant gene has the following mutation: the 1731st siteor/and 2276th site of the amino acid sequence corresponding to the wild type rice ACCase is subjected to mutation. The ACCase mutant gene is introduced into herbicide-sensitive rice genome by a transgenic method and is expressed; and receiver plants can have resistance to acetyl-CoA carboxylase inhibitor herbicide. The ACCase mutant gene can be widely applied to the breeding of plants with the herbicide resistance.

The invention provides a specific PCR (polymerase chain reaction) primer pair for detecting ALS (acetolactate synthetase) inhibitor herbicide-resistant descurainia sophia. The primer pair comprises a primer pair (Seq ID No.1-2) for specific amplification of a B-ALS-1 gene of the descurainia sophia and a primer pair (Seq ID No.3-4) for specific amplification of a B-ALS-2 gene of the descurainia sophia. A PCR detection method adopting the primer pair is excellent in specificity and sensitivity, and a specificity test proves that the two ALS genes of the descurainia sophia can be amplified (figures 1 and 2). As the PCR method is simple, convenient and quick to operate, the sampling detection can be carried out in season based on nucleotide detection, and only 2-4 days are required for a process from sampling to results. The sensitivity and specificity of the method can realize quick identification of suspended ALS inhibitor herbicide-resistant descurainia sophia in the field and confirmation of mutation sites, thus the scientific control of resistant weeds in production practice is guided.

The invention belongs to the field of pesticides, and particularly relates to weeding composition containing pyrazolone compounds and an application of the weeding composition. The weeding composition comprises an effective weeding quantity of an active component A, an active component B and a safener compound C, wherein the active component A is one of general formulae represented in the specification, R1 represents hydrogen atoms, R2 represents methyl, R3 represents ethyoxyl, R4 represents helium atoms, and R5, R6 and R7 represents alkyl and the like; the active component B is selected from one or more of compounds as follows: 1) a triazine compound: ametryn; 2) diphenyl ether compounds: oxyfluorfen and the like; the safener compound C is selected from one or more of compounds as follows: C1: isoxadifen-ethyl and the like. With the adoption of the composition, the problems about weeds including barnyard grass, eleusine indica, horseweed herb and the like during destructive weeding and sugarcane field weeding can be solved effectively, and the composition has the characteristics that the weeding spectrum is enlarged, the application amount is reduced, the composition is safe to crops, herbicide resistant weeds can be controlled and the like.

The invention relates to a herbicide-resistant protein as well as an encoding gene and application thereof. The herbicide-resistant protein comprises: (a) a protein constituted by an amino acid sequence shown as SEQ ID NO: 2, or (b) a protein which is obtained by performing substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence in (a), has herbicide resisting activity and is derived from (a). The herbicide-resistant protein is particularly suitable for expression in plants, and is highly resistant to herbicides, especially a phenoxyl auxin herbicide.