45 results about "Herbicide resistant" patented technology

The present application relates to a kind of deletion mutant nucleic acid and its application in anti-herbicide.The deletion mutation occurs in the promoter of rice OsHPPD Gene, the length of deletion sequence is at least 10 bp, and the A in the start codon ATG of gene is 0, at least the bases located in the upstream of start codon ATG from 2041 to 2032 are deleted. OsHPPD The present application finds that by deleting part of the sequence in the promoter of rice OsHPPD Gene, rice can obtain resistance to HPPD inhibitor herbicides, which is of great application value for ensuring agricultural production safety and improving the efficiency of herbicide use.

The invention relates to the technical field of gene editing, and particularly provides a method for creating herbicide-resistant rice through gene editing of an OsEPSPS gene promoter region, and the method is characterized in that WT / 29bp single allelic deletion mutation is introduced into the OsEPSPS gene promoter region, i.e., A in an OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, and A in the OsEPSPS gene initiation codon ATG is 0 site; 29bp is deleted from the 112nd site to the 1150th site at the upstream of the initiation codon ATG on one allele, and the other allele is kept unchanged. By introducing the single allelic deletion mutation, the rice can obtain resistance to EPSPS inhibitor herbicides, the resistance is identified in T0-generation plants carrying the mutation and can be stably inherited to offspring plants, and the application value is high for guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

The application discloses a P450 enzyme mutant for degrading herbicides and application thereof, and the P450 enzyme mutant is named P450 BSβ -F46A, P450 BSβ -F46A-CPR; the amino acid sequences are shown in SEQ ID NO. 2 and 4 respectively. The application also discloses application of the P450 enzyme mutant in degrading pyridine oxycarboxylic acid herbicides or aryloxyphenoxypropionic acid herbicides and related genes in cultivating herbicide-resistant transgenic crops. Experiments prove that the highest conversion rate of the P450 enzyme mutant for degrading pyridine oxycarboxylic acid herbicides reaches 86%, and the total conversion number of catalyzing flumiclorac can reach 7443 at most, and the highest catalytic conversion rate of degrading aryloxyphenoxypropionic acid herbicides reaches 89%. The P450 enzyme mutant provided by the application has the advantages of high catalytic efficiency, high protein expression amount and low industrial cost, and the whole operation process for degrading herbicides is simple, the process is mature, the cost is low, and the application prospect is wide.

This invention relates to the field of gene editing technology, specifically providing a method for creating herbicide-resistant rice by gene editing the promoter region of the OsEPSPS gene. The method involves introducing a WT / -29bp monoallelic deletion mutation into the OsEPSPS gene promoter region. Specifically, with position A of the OsEPSPS gene start codon ATG set to 0, a 29bp deletion is made in one allele located at positions 1122 to 1150 upstream of the start codon ATG, while the other allele remains unchanged. This invention demonstrates that by introducing this monoallelic deletion mutation, rice can acquire resistance to EPSPS inhibitor herbicides. This resistance was identified in the T0 generation plants carrying this mutation and can be stably inherited by their offspring, possessing significant application value for ensuring agricultural production safety and improving herbicide application efficiency.

This invention discloses a breeding method for improving japonica rice by cultivating polymorphic lines. This invention integrates the GS3 long-grain gene, PigmR disease-resistant gene, fgr aroma gene, ALS herbicide-resistant gene, and COLD1 cold-tolerant gene into japonica rice. The GS3 long-grain trait successfully compensates for the reduction in thousand-grain weight caused by the PigmR gene. Simultaneously, the COLD1 cold-tolerant genotype repairs the genetic defect of low-temperature intolerance in the GS3 donor parent. Furthermore, it demonstrates the heat-resistant function of GS3 in the context of polymorphic lines, simultaneously improving grain shape, blast resistance, aroma, herbicide resistance, heat tolerance, and low-temperature tolerance. This provides new germplasm for the green and efficient production of japonica rice and has significant application value.

The invention relates to the technical field of gene editing, and particularly provides rice OsEPSPS gene promoter region deletion mutation and application thereof in creation of herbicide-resistant rice, the deletion mutation is that a sequence with the length of 22bp or 28bp is deleted in the OsEPSPS gene promoter region, namely, A in an OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, and A in the OsEPSPS gene initiation codon ATG is 0 site; and deleting the basic groups at sites 2534 to 2555 upstream of the initiation codon ATG or the basic groups at sites 2527 to 2554 upstream of the initiation codon ATG. According to the application disclosed by the invention, the rice can obtain resistance to EPSPS inhibitor herbicides by deleting part of sequences in the promoter of the rice OsEPSPS gene, so that the application has great application values in guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

The invention relates to a rice OsACC gene promoter mutant for resisting ACCase inhibitor herbicides and application of the rice OsACC gene promoter mutant. The rice OsACC gene mutant is a deletion mutant or a deletion and combination insertion mutant; the deletion mutation occurs in the promoter of the rice OsACC gene, and the sequence of the nucleic acid before deletion mutation is as shown in SEQ ID No. 1; the length of the sequence of the deleted nucleic acid is 29 bp to 97 bp, A in the initiation codon ATG of the OsACC gene is 0 site, the deleted nucleic acid occurs in the 305-209 sites of the upstream of the initiation codon ATG, and at least the 271-243 sites of the upstream of the initiation codon ATG are deleted; the inserted nucleic acid occurs within sites 271 to 243 upstream of the initiation codon ATG. The mutant can enable rice to obtain resistance to at least one of herbicides such as haloxyfop-R-methyl, fluazifop-p-butyl and clethodim.

This invention discloses a herbicide-resistant cytochrome P450 gene, BjCYP71C2, its protein, plasmid, and their applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene is SEQ ID NO:1 or has at least 80% identity with SEQ ID NO:1. This invention also discloses the protein polypeptide and plasmid encoded by the above gene. The purpose of this gene is to express it transgenic in plants, cultivating herbicide-resistant plants for selective weed control using herbicides.

A method for controlling PPO resistant weeds, wherein compounds of formula (I) wherein the variables are defined as given in the description and claims; are applied to the PPO inhibitor herbicide resistant weed, parts of it or its propagation material.

The invention discloses a EcCYP81A105 gene and application thereof, and belongs to the technical field of plant biology, the nucleotide sequence of the EcCYP81A105 gene is shown as SEQ ID NO.1, and the amino acid sequence of the EcCYP81A105 gene is shown as SEQ ID NO.2. The invention finds that the EcCYP81A105 gene has the function of improving the drug resistance of rice to ACCase herbicides for the first time, and can be used as a resistance gene for cultivating herbicide-resistant rice germplasm.

The invention discloses a herbicide-resistant gene StGSTF8 in sagittaria trifolia and application of the herbicide-resistant gene StGSTF8, and belongs to the technical field of genetic engineering. The bensulfuron methyl resistance gene StGSTF8 (glutathione-S-transferase F8) is identified from sagittaria trifolia for the first time by means of proteomics, molecular biology, transgenic function verification and the like, the nucleotide sequence is as shown in SEQ ID NO: 1, the coded amino acid sequence is as shown in SEQ ID NO: 2, and the nucleotide sequence is as shown in SEQ ID NO: 1. It is proved that the strain endows plants with resistance through mediating herbicide detoxification metabolism and oxidative stress resistance, and reference is provided for improvement of the resistance of plants to sulfonylurea herbicides and research and development of novel herbicides.

The invention discloses an application of rice OsTIP2; the invention discloses application of the gene 1, 2 in improving the resistance of rice to acetochlor and a rice breeding material thereof. The method comprises the following steps: knocking out OsTIP2; according to the OsTIP2 gene, the resistance of rice to acetochlor can be improved; the amino acid sequence of the gene 1, 2 is as shown in SEQ ID NO: 2; and by matching with acetochlor for use, simplified planting of the rice can be assisted, and important economic value is achieved. The herbicide resistance characteristic of rice edited by the gene can be stably inherited through sexual or asexual reproduction, and the gene can be used for constructing resistant rice germplasm resources.

The invention relates to the technical field of gene engineering, in particular to an OsHSL1 protein mutant and application of the OsHSL1 protein mutant to improvement of herbicide resistance of plants. The amino acid sequence of the OsHSL1 protein mutant comprises any one or more of the following mutations on the basis of the amino acid sequence of the OsHSL1 protein: (1) F140H and Q255P; and (2) L90M, F140H, L204F, and F298L, which are selected from the group consisting of a group consisting of Two OsHSL1 protein mutants are obtained through research and screening, and compared with the degradation activity of the OsHSL1 protein mutants before mutation, the degradation activity of the OsHSL1 protein mutants on herbicides is remarkably improved. The OsHSL1 protein mutant provided by the invention can also be used for cultivating plant varieties with high herbicide resistance, which has important application value in the field of plant breeding.

The invention relates to a method for breeding a rice blast-resistant and herbicide-resistant japonica glutinous rice variety by overcoming Pigm adverse effects. A glutinous rice variety which is widely popularized in production and has excellent comprehensive characters is selected as a receptor parent, a broad-spectrum rice blast-resistant gene Pigm and a herbicide-resistant gene ALS are screened under the assistance of molecular markers, and the rice blast resistance gene Pigm and the herbicide-resistant gene ALS are obtained through backcross, selfing, multiple cross and phenotype screening based on thousand seed weight. The thousand seed weight of the japonica glutinous rice material is 26.5 g or above, and the japonica glutinous rice material is polymerized with broad-spectrum rice blast-resistant and herbicide-resistant genes. The glutinous rice new strain bred by the method successfully integrates two key genes of broad-spectrum high rice blast resistance Pigm and imidazolone herbicide resistance ALS, and main agronomic characters such as thousand seed weight, maturing rate, plant and leaf morphology and the like are equivalent to or better than those of an excellent receptor parent.

The application relates to the technical field of gene editing, and provides a method for creating herbicide-resistant rice through gene editing of an intron region of an OsEPSPS gene, specifically, a WT / -22bp single allelic deletion mutation or a WT / -32+6bp single allelic deletion insertion mutation is introduced into the intron region of the OsEPSPS gene, that is, taking A in an initiation codon ATG of the OsEPSPS gene as the 0 position, 22bp is deleted from 771 to 792 downstream of the 0 position on one allele or 32bp is deleted from 775 to 806 downstream of the 0 position and 6bp is inserted into the interval, and the other allele remains unchanged. It is found by the application that the rice can obtain resistance to EPSPS inhibitor herbicides by introducing the above-mentioned mutation, the resistance is identified in T0 generation plants carrying the mutation, and can be stably inherited to the offspring plants, and the application value for improving the use efficiency of herbicides is extremely high.

The application belongs to the technical field of herbicide resistance genes, and relates to a rice herbicide-resistant gene and application. OsCYP704A5 The sequence is shown as SEQ ID NO. 1. The application carries out molecular docking analysis on the gene and triazolopyrimidine sulfonamide, and indicates that OsCYP704A5 has high binding capacity with triazolopyrimidine sulfonamide and has certain promoting effect on detoxification metabolism. OsCYP704A5 Further overexpression of the gene in Nipponbare rice can make the transgenic rice obtain triazolopyrimidine sulfonamide resistance. OsCYP704A5 The application can be used for cultivating HPPD inhibitor herbicide-resistant rice varieties and provides new possibilities for studying rice field weed (barnyard grass) resistance to HPPD inhibitor herbicides.

The invention relates to the technical field of gene editing. Specifically, the invention provides a method for creating herbicide-resistant rice through gene editing of a 3 'UTR region of an OsEPSPS gene, and the method is characterized in that-38 / -13 + 12bp double allelic deletion insertion mutation is introduced into the 3' UTR region of the OsEPSPS gene, i.e., A in an initiation codon ATG of the OsEPSPS gene is taken as a 0 site, two alleles are respectively positioned at the 3363 to 3400 site deletion 38bp of the downstream of the OsEPSPS gene and at the 3377 to 3389 site deletion 13bp of the downstream of the OsEPSPS gene, and 12bp is inserted in the section. It is found that by introducing the double allelic deletion insertion mutation, rice can obtain resistance to EPSPS inhibitor herbicides, the resistance is identified in T0-generation plants carrying the mutation and can be stably inherited to offspring plants of the T0-generation plants, and the application value for guaranteeing agricultural production safety and improving the use efficiency of the herbicides is extremely high.

The application discloses an anti-herbicide gene LcAKR4C11, a protein, a plasmid and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO:1 or a sequence with not less than 80% homology with SEQ ID NO:1. The application further discloses a protein polypeptide and a plasmid encoded by the anti-herbicide gene. The anti-herbicide gene is used for: cultivating a plant with anti-herbicide and anti-oxidation capability by transgenic expression in a plant, and selectively preventing weeds by using herbicides.

The present invention pertains to the fields of agricultural biotechnology or plant biotechnology. Specifically, it relates to the genetic modification of plants, in which genes of interest are inserted to impart desirable characteristics thereto. The present invention relates, in particular, to a method for obtaining genetically modified Agave sisalana (A. sisalana) plants. More specifically, the method according to the invention relates to the development of an unprecedented protocol for obtaining transgenic A. sisalana plants via infection by Agrobacterium tumefaciens (A. tumefaciens), with increased efficiency in the creation of transgenic plants.

The invention relates to a herbicide-resistant mulberry new germplasm creation and optimal matching rapid propagation technology, which comprises the following steps: constructing 86 resistant single plants by 180Gy electron beam irradiation, and distinguishing the 86 resistant single plants into a stock type and a scion type according to root biomass and a leaf resistance index; performing two-stage screening on 1260 groups of excessive matching groups to obtain 3 sets of optimized combinations with the death rate reduced by more than or equal to 15%; a double-backup mechanism of'main part + 2 parts of alternative parts' is established, and annual rapid propagation of 150000 plants is realized by means of cloud collaboration. The method fills the blank of mulberry resistance breeding matching optimization and risk prevention and control.

The invention discloses a method for improving gene expression and plant herbicide resistance, and particularly relates to a method for improving the expression level of endogenous polynucleotide or a target gene by modifying upstream and downstream of a transcription start site of the target gene through a genome editing technology and modifying a non-coding region of the gene. Furthermore, the HPPD gene and / or HIS1 gene related to the herbicide resistance of the plant are / is modified by the method to regulate and control, so that the herbicide resistance of the plant is improved.

The invention relates to the technical field of gene editing, and provides a method for creating herbicide-resistant rice through gene editing of an intron region of an OsEPSPS gene, and particularly relates to a method for introducing single allelic deletion mutation of WT / 22bp or single allelic deletion insertion mutation of WT / 32 + 6bp into the intron region of the OsEPSPS gene, i.e., A in an initiation codon ATG of the OsEPSPS gene is 0 site, A in the initiation codon ATG of the OsEPSPS gene is 0 site, A in the initiation codon ATG of the OsEPSPS gene is 0 site, and A in the initiation codon ATG of the OsEPSPS gene is 0 site; on one allele, 22bp is deleted from the 771 site to the 792 site at the downstream of the allele or 32bp is deleted from the 775 site to the 806 site at the downstream of the allele, and 6bp is inserted in the interval, and the other allele is kept unchanged. It is found that by introducing the mutation, rice can obtain resistance to EPSPS inhibitor herbicides, the resistance is identified in T0-generation plants carrying the mutation and can be stably inherited to offspring plants of the T0-generation plants, and the application value for improving the use efficiency of the herbicides is extremely high.

This invention belongs to the field of biotechnology, specifically relating to a biocontrol agent for alfalfa fields resistant to the herbicide *Amaranthus retroflexus* and its application. The biocontrol agent contains a suspension of mycelium and / or spores of *Morchella* strain MXBP304 as its active ingredient. The microbial strain is MXBP304, belonging to the genus *Morchella*. Mortierella sp The fungus, accession number MXBP304, was deposited on December 22, 2021, at No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, with accession number CCTCC NO:M 20211660. The concentration was 2.8 × 10⁻⁶. 7 A CFU / mL spore suspension significantly inhibited seed germination of *Amaranthus retroflexus*, with a relative germination rate of 27.45% and a significant decrease in root length of 29.57%. Meanwhile, this treatment had no adverse effects on seed germination, growth, or major nutritional quality of alfalfa, demonstrating excellent selectivity and growth-promoting effects.

Provided are compositions and methods relating to gene and / or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant.

The invention discloses a KASP molecular marker for auxiliary screening of mesosulfuron-resistant character of wheat and application of the KASP molecular marker in identification or auxiliary identification of the mesosulfuron-resistant character of the wheat. The KASP molecular marker can be used for detecting the genotype of the A101G SNP site in a wheat genome, and can be used for rapidly and accurately screening out a wheat variety with high tolerance to mesosulfuron-methyl, so that the breeding process and efficiency of a new herbicide-resistant wheat variety are remarkably improved. The A101G SNP site is located at the 101 basic group from the 5'tail end of the sequence shown in SEQ ID NO: 1, and comprises two allele types, namely an AA homozygous type and a GG homozygous type. A large number of wheat tests prove that the tolerance of the wheat with the genotype of the A101G SNP site being a GG homozygous type to mesosulfuron-methyl is higher than that of the wheat with the genotype being a CC homozygous type. The invention provides a standardized and high-throughput molecular detection technical scheme for genetic improvement of wheat drug resistance, and is of great significance to guarantee safe use of wheat field mesosulfuron-methyl.

A method for increasing gene expression and improving herbicide resistance in plants. Specifically, the method involves performing modifications at upstream and downstream regions of a transcription start site of a gene of interest by means of a genome editing technique, so as to modify a non-coding region of the gene, thereby increasing the expression level of an endogenous polynucleotide or the gene of interest. Furthermore, the method is used to modify and regulate an HPPD gene and / or HIS1 gene associated with herbicide resistance in the plants, thereby improving herbicide resistance in the plants.

The invention belongs to the field of plant anti-herbicides, and discloses a plant anti-paraquat herbicide PUT mutant protein and application thereof. According to the invention, transmembrane structural domain analysis is carried out on proteins PUT1, PUT2 and PUT3 of paddy rice, so that potential mutation sites PUT1G458, PUT2G359 and PUT3G444 of the paddy rice OsPUT1 / 2 / 3 gene are predicted. According to the present invention, the OsPUT1 / 2 / 3 gene is subjected to precise mutation by using the guide editing system method, the conserved site is mutated into the positively charged PUT1G458R, PUT2G359R or PUT3G444R so as to successfully obtain the target mutant plant, and the paraquat resistance identification is performed to finally create the paraquat-resistant rice germplasm, such that the important significance is provided for the diversity of the rice germplasm resource.

The invention discloses a herbicide-resistant glycosyl transferase gene BsUGT74B1, a polypeptide, a plasmid and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a herbicide-resistant beckmannia syzigachne variety is firstly found, then the UGTs gene BsUGT74B1 is cloned by using the information of the sequence of the herbicide-resistant beckmannia syzigachne variety, and the herbicide resistance of the BsUGT74B1 gene and the application of the BsUGT74B1 gene in development of transgenic herbicide-resistant crops are further proved in rice. The invention aims to provide a novel molecular tool for breeding of herbicide-resistant crops and pesticide resistance management by identifying and utilizing the UGTs gene.