The present invention relates to the production of a non-transgenic
plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g.,
glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired
mutation in the chromosomal or episomal sequences of a
plant in the
gene encoding for 5-
enol pyruvylshikimate-3-
phosphate synthase (EPSPS). The
mutated protein, which substantially maintains the catalytic activity of the wild-type
protein, allows for increased resistance or tolerance of the
plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially
normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic
plant cell in which the EPSPS
gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS
gene. The amino acids at the positions 126, 177, 207, 438, 479, 480 and / or 505 are changed tp produce a
mutant EPSPS
gene product.