276 results about "Carboxysome" patented technology

The present invention provides compounds useful as inhibitors of Acetyl CoA Carboxylase (ACC), compositions thereof, and methods of using the same.

In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.

The invention relates to a foliar fertilizer used for enhancing the photosynthesis of plants, and its preparation method. The foliar fertilizer which is a composition comprises a part A and a part B, the part A comprises 1-3% of chitosan, 1-3% of ribulose-1,5-bisphosphate carboxylase, 5% of a surfactant, 1-5% of an antifreeze agent, 5-10% of acetic acid, 10% of ethanol, 0.1-0.2% of a stabilizer, and the balance water, and the pH value of the part A is 4-6; the part B comprises 5-5% of sodium bisulfate, 5-10% of biochemical fulvic acid, 1-5% of the antifreeze agent, 5% of the surfactant, 5-10% of potassium dihydrogen phosphate, 5-10% of urea, 1-3% of magnesium sulfate, 0.1-0.2% of a stabilizer, and the balance water, and the pH value of the part B is 7-8; a ratio of the part A to the part B is 1:1; and materials of the part A and materials of the part B are uniformly stirred and mixed at normal temperature to form the composition, and the composition is diluted to 1000-2000 times aqueous solution spray when the composition is used. The foliar fertilizer can effectively solve the problems of bad color and low quality caused by possible-meeting of bad natural environment after too late bag removal of bagged apples.

The invention discloses a corynebacterium glutamicum strain for production of 5-aminolevulinic acid and construction and application of corynebacterium glutamicum strain. A construction method includes: (1) deleting a lactic dehydrogenase coding gene 1dhA and acetic acid generation genes pta-ackA, pqo and cat in corynebacterium glutamicum to obtain a strain named CB4; inserting a strong sod promoter in front of a phosphoenolpyruvate carboxylase coding gene ppc in the strain CB4 to obtain a strain CB5; deleting a gene pck in the strain CB5 to obtain a strain CB6; (2) transferring plasmid pXA and plasmid pEP2<tuf>-rhtA into the strain CB6. The strain constructed according to the method is capable of generating 2.78g/L 5-aminolevulinic acid in a culture medium with 10g/L glucose serving as a carbon source, which lays a foundation for subsequent continuous feeding of a fermentation tank to increase yield of the 5-aminolevulinic acid.

The invention discloses a rice ACCase mutant gene and an application thereof in herbicide resistance of plants. The amino acid sequence of the encoding protein of the ACCase mutant gene has the following mutation: the 1731st siteor/and 2276th site of the amino acid sequence corresponding to the wild type rice ACCase is subjected to mutation. The ACCase mutant gene is introduced into herbicide-sensitive rice genome by a transgenic method and is expressed; and receiver plants can have resistance to acetyl-CoA carboxylase inhibitor herbicide. The ACCase mutant gene can be widely applied to the breeding of plants with the herbicide resistance.

The invention rice photosynthesis key enzyme-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large-subunit antigen epitope, a polyclonal antibody resisting rice Rubisco large-subunit, and a preparation method and applications of the antibody. The amino acid sequence of the rice Rubisco large-subunit specific antigen epitope is as shown in SEQ ID NO:1. The preparation method comprises the following steps: synthesizing a polypeptide; coupling the polypeptide with carrier protein; preparing antiserum from an immune animal; and performing affinity chromatography for purification, thereby obtaining the polyclonal antibody resisting rice Rubisco large-subunit. The polyclonal antibody is high in titer, remarkable in affinity, excellent in specificity, low in preparation cost and high in yield, and can generate specific binding reaction with rice Rubisco large-subunit; an important tool is supplied for the fundamental research on the rice Rubisco large-subunit as well as the research on the relevant physiological functions of the rice Rubisco large-subunit.

The invention discloses a method for knocking out pckA to promote synthesis of acetylglucosamine through bacillus subtilis and belongs to the field of genetic engineering. The method comprises the steps that BSGNK-PxylA-glmS-P43-GNA1 serves as original strains, phosphoenolpyruvate carboxylase (pckA) genes are knocked out through homologous recombination, the reaction that enolphosphopyruvate is converted into oxaloacetic acid in host bacterium cells is blocked, the carbon flux flowing to a tricarboxylic acid cycle is reduced, and accumulation of acetylglucosamine is promoted. In the process that a composite culture medium is used for fermentation, the acetylglucosamine yield of recombination bacillus subtilis with pckA knocked out reaches 29.27 g/L and is increased by 28.1% compared with that of control strains. The method lays a foundation for transforming bacillus subtilis to produce acetylglucosamine in metabolic engineering.

The invention discloses construction and applications of a corynebacterium glutamicum mutant strain for producing L-homoserine, and belongs to the technical field of fermentation engineering. Corynebacterium glutamicum ATCC 13032 is taken as a starting strain to knock out regulatory protein McbR, homoserine kinase, transport protein MetD, phosphoenolpyruvate carboxykinase; the expression of isocitrate dehydrogenase is down regulated; transport protein BrnFE, aspartic semialdehyde dehydrogenase and homoserine dehydrogenase are overexpressed; and the expression of aspartate kinase, pyruvate carboxylase and the aspartate kinase I derived from escherichia coli are enhanced. Shake flask culture can be performed on the mutant strain for 48 h, and the yield of L-homoserine can reach 8.8 g/L.

The invention relates to a new fragmentation chytrid variant. Especially, the invention relates to a fragmentation chytrid variant with improved DHA (docosahexaenoic acid) content and/or rapid growth speed and a method for producing DHA by utilizing the variant. Besides, the invention also relates to a method for producing the variant, comprising the steps of carrying out mutagenesis on fragmentation chytrid by adopting ultraviolet rays and screening a strain with rapid growth speed and high DHA content under selective pressure of an inhibitor (such as quizalofop) for fat synthesis key enzyme (acetyl coenzyme A carboxylase).

The present invention provides for compositions and methods for producing crop plants that are resistant to herbicides. In particular, the present invention provides for wheat plants, plant tissues and plant seeds that contain altered acetyl-CoA carboxylase (ACCase) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ACCase protein.

The invention discloses a genetically engineered bacterium for directly producing L-aspartic acid through fermentation. The classification naming of the genetically engineered bacterium is Escherichia coli CM-AS-115, and the preservation number of the genetically engineered bacterium is CCTCC NO: M 2016457. The bacterial strain relates to inactivation of multiple genes, evolution, metabolism and domestication are simultaneously carried out on the bacterial strain which knockouts the multiple genes, and a mutant strain, namely, CM-AS-105, which has a lower respiratory quotient under the aerobic condition and of which the highest dry cell weight is 60-70% of dry weight of an original strain W1485 is obtained; meanwhile, the bacterial strain further relates to over expressions of two genes, wherein the two genes comprise an enol phosphate type pyruvate carboxylase encoding gene (ppc) and an aspartase encoding gene (aspA), and the obtained bacterial strain is CM-AS-115. The genetically engineered bacterium for directly producing L-aspartic acid through fermentation achieves a way of completely adopting renewable biomass resources such as starch and cellulose as raw materials to ferment and prepare the L-aspartic acid, and the way is green and environmentally friendly.

The invention in particular relates to a plant expression vector pH2-35S-PrbcS-AMDH for improving the aluminum toxicity resistance of plants, a construction method and application thereof, which belong to the field of plant gene engineering. The special vector pH2-35S-PrbcS-AMDH for improving the aluminum toxicity resistance of the plants is the plant expression vector containing a photoinducible promoter (PrbcS) of a rubulose-1, 5-bisphosphate carboxylase (RubIsco) small subunit gene and an arabidopsis thaliana cytosolic malate dehydrogenase gene (AMDH). The AMDH gene is cloned from arabidopsis thaliana, the photoinducible promoter is used to control the overexpression of the AMDH gene in tobacco, malic acid is synthesized, and the malic acid is secreted out of cells so as to strengthen the resistance of the plants on aluminum toxicity in acid soil. Experimental results show that the activity of malate dehydrogenase of trans-AMDH genic tobacco leaves is 1.4 times of that of wild tobacco. Under the stress of 30 mu M of aluminum toxicity, trans-AMDH genic tobacco can secrete more organic acid, and has better root system growth; and the growth condition under the stress of the aluminum toxicity shows that the plant height and the green leaf number of the trans-AMDH genic tobacco are higher than those of the wild tobacco.

The invention discloses a corynebacterium glutamicum engineering bacterium for anaerobic conversion to produce succinic acid, and a building method and application thereof, and belongs to the field of genetic engineering. A pyruvate carboxylase gene of the corynebacterium glutamicum and phosphoenolpyruvate carboxylase from escherichia coli are cloned to corynebacterium glutamicum (ATCC13032); a lactate dehydrogenase gene of the corynebacterium glutamicum is knocked out in a homologous recombination manner. By adopting the lactic dehydrogenase-defective corynebacterium glutamicum for coexpression of a carboxylase gene, anaerobic production of succinic acid is carried out in a cell reutilization manner, so that the yield of succinic acid can be greatly improved; the yield can be up to 75g/L; the conversion rate of saccharic acid is 75%; the corynebacterium glutamicum engineering bacterium has a good application prospect; a fermentation model, especially a fermentation model for cell reutilization is built according to the optimum condition for biological transformation of succinic acid; the acid-production performance in repeated batch transformation process of cells can be basically kept stable.

The invention provides a genetic engineering bacterium strain for producing succinic acid. The genetic engineering bacterium strain is named as Escherichia coli BA016, the preserving number registration number CCTCC NO is M 2012350. The invention further provides a construction method of the strain and a method for producing succinic acid by fermentation, recombinant escherichia coli can grow by glucose metabolism through joint excessive expression of exogenous pyruvic carboxylase and nicotinic acid ribose phosphate transferase, generation of by-product pyruvic acid is reduced, and accordingly the yield and production intensity of succinic acid are greatly improved.

The invention belongs to the field of biology engineering technology, and relates to a genetic engineering strain for producing succinic acid by utilizing glucose and an acidogenic fermentation method of the genetic engineering strain. The genetic engineering strain for producing succinic acid by utilizing glucose is named as Escherichia coli BA205 and the preservation number is registered as CCTCC No.M2011447. In the construction process, Escherichia coli which is short of lactic dehydrogenase (LDH) gene and Pyruvate formate-lyase (PFL) gene activity is mainly used as an original strain; phosphoenolpyruvate carboxylase (PPC) gene is removed by utilizing a homologous recombination technology; and phosphoenolpyruvate carboxylase and nicotinic acid phosphoribosyl transferase are excessively co-expressed; therefore the synthesis efficiency of succinic acid is greatly increased. In the fermentation method, a two-stage fermentation manner is adopted, the biomass is improved in an aerobic stage and the acidogenic fermentation is carried out in an anaerobic stage.

The invention discloses rice ACCase mutant protein. An amino acid sequence of the ACCase mutant protein has one or more types of mutations corresponding to mutations of the 1796th locus, the 1797th locus, the 1835th locus, the 1875th locus, the 2010th locus, the 2050th locus, the 2060th locus, the 2070th locus and the 2106th locus of a wild-type rice ACCase amino acid sequence. The invention further discloses a nucleic acid or a gene which codes the protein. By a transgenic method, the mutant gene is expressed in herbicide-sensitive rice, and plants containing the mutant protein are resistantto acetyl coenzyme A carboxylase herbicides. After application of 3mL quizalofop-ethyl/L water (twice of recommended concentration) to three-leaf rice seedlings containing the rice ACCase mutant protein, the plants still grow, develop and fruit normally.

The invention relates to a plant expression vector pH2-35S-PrbcS-adh of arabidopsis glutathione-dependent formaldehyde dehydrogenase gene, which is the plant expression vector of light-induced promoter (PrbcS) containing tomato Rubisco 3C small subunit gene and the arabidopsis glutathione-dependent formaldehyde dehydrogenase (FALDH) gene adh. The gene adh is amplified from arabidopsis, and the over expression of the gene adh in tobacco lamina cytoplasm is controlled by the light-induced promoter, so that the efficiency of decomposing formaldehyde by tobacco cell can be improved, thus enhancing the capability of absorbing and enduring exogenous formaldehyde by plant. The experiment proves that enzyme activity of adh transgenic tobacco FALDH is increased by about 3-5 times compared with that of wild type; after being cultured for 30 days on an MS solid culture medium containing 10mmol/L of formaldehyde, the growing situation of the adh transgenic tobacco is obviously superior to that of wild type. In addition, when 2mM formaldehyde liquid is used for treating the plant, the formaldehyde absorption rate of the adh transgenic tobacco is obviously higher than that of wild type.