The invention provides an artificial non-coding
RNA (Ribonucleic Acid) molecule,
a DNA (
Deoxyribose Nucleic Acid) molecule, a biological material and application of the artificial non-coding
RNA molecule, the
DNA molecule and the biological material in improving the carbon and
nitrogen metabolism capability of
rhizobium, and belongs to the technical field of
genetic engineering, the
nucleotide sequence of the artificial non-coding
RNA molecule is as shown in SEQ ID NO.1, and the
nucleotide sequence of the
DNA molecule transcribing the artificial non-coding
RNA molecule is as shown in SEQ ID NO.2; the invention also provides a recombinant vector and recombinant
rhizobium with efficient carbon and
nitrogen metabolism capability. According to the artificial non-coding
RNA molecule and the
recombinant expression vector constructed by using the
RNA molecule, the utilization capacity of
malic acid of the
rhizobium can be remarkably improved, then the symbiotic
nitrogen fixation capacity of the rhizobium is enhanced, the rhizobium and leguminous crops are symbiotic, and the yield and quality of the leguminous crops can be remarkably improved.