71 results about "Pathogen resistance" patented technology

The invention discloses a chelerythrine-containing compound microbial agent and application thereof, and belongs to the technical field of biological control. The invention provides a chelerythrine-containing compound microbial agent to solve the technical problems that in the prior art, only a single microbial agent and a plant extract are used, the biological control effect is poor, the stability is low, and the target spot is single. The compound microbial agent is prepared from a mortierella alpina extract, a trichoderma hairy extract and chelerythrine according to a specific compounding ratio. The compound microbial agent provided by the invention can delay the development of drug resistance of pathogenic bacteria through a multi-target effect, has better stability and stronger control effect, reduces the use amount of chemical pesticides, reduces the harm to the environment and human body, can be applied to tobacco planting production, can effectively reduce the control cost of tobacco black shank, and has broad application prospects. The economic benefit of tobacco planting is improved.

The invention discloses a method and system for detecting respiratory tract pathogens, pathogen drug-resistant genes and pathogen virulence genes, and relates to the technical field of gene detection.The method comprises the steps that a respiratory tract sample of a patient is collected, DNA and RNA are extracted, and total nucleic acid is obtained; carrying out first-round PCR amplification on the total nucleic acid, purifying a first-round PCR product, carrying out second-round PCR amplification, and sorting the product to obtain a library building product; performing high-throughput sequencing on the library building product to obtain sequencing data, and detecting pathogens, drug-resistant genes and virulence genes; according to the method and system for detecting the respiratory tract pathogens, the pathogen drug-resistant genes and the pathogen virulence genes, by optimizing library preparation and direct library building of nucleic acid, the process is simplified, and efficiency and accuracy are improved; the balance reagent design guarantees library quality; the kit adapts to pathogen detection requirements in Yunnan and the like, provides efficient diagnosis, shortens detection time, improves treatment effect, regularly updates pathogen information, and keeps technical frontier.

The present invention relates to novel tomato plants having improved resistance to lesion-forming pathogens caused by a reduced level, activity, or expression of Pub17 protein. The level, activity, or expression of a Pub17 protein can be reduced by modification of the Pub17 allele to contain an SNP identified to associate with increased resistance to lesion-forming pathogens. The present invention further relates to plant parts and seeds derived from said tomato plants, and to methods of making said tomato plants or increasing resistance to lesion-forming pathogens in a tomato plant, as well as use of the SNP as a marker and associated kits.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust and / or powdery mildew) in a plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

Methods and compositions for identifying, selecting and / or producing a pathogen resistant plant, plant cell or seed (e. g,, a maize plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and / or pathogen resistance (e.g., to Fusarium stalk rot) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

Synthetic Southern Phaseoloviridae satellite RNA molecules and satellite particles containing the same are disclosed. Synthetic Southern Phaseoloviridae satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon are also disclosed. Also disclosed are methods of using Southern Phaseoloviridae satellite RNA molecules and satellite particles containing the same to alter plant phenotypes, improve stress resistance in plants, and improve pest and pathogen resistance in plants.

The disclosure relates to a plant that is tolerant or resistant to species of Ca. Liberibacter. Specifically exemplified are Citrus and solanaceous plants. Provided by the disclosure is a modified Citrus or solanaceous plant that is resistant or tolerant to Sec-dependent effectors secreted by bacteria. Also provided by the disclosure are methods of modifying a plant genome plant to provide tolerance or resistance to species of Ca. Liberibacter. Still further provided by the disclosure are methods conferring a population of plants with tolerance or resistance to species of Ca. Liberibacter and screening that population for the plants that are tolerant or resistant to species of Ca. Liberibacter.

Methods and compositions for identifying, selecting and / or producing a pathogen resistant plant, plant cell or seed (e.g., a legume or soybean plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and / or pathogen resistance (e.g., to Asian Soy Rust) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.

Disclosed are synthetic Marivirus eye satellite RNA molecules and satellite particles containing the same. Also disclosed are synthetic mateur virus satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon. Also disclosed are methods of altering plant phenotypes, improving stress resistance in plants, and improving pest and pathogen resistance in plants using the Marivirus eye satellite RNA molecules and satellite particles containing the same.

The invention relates to the technical field of pesticide compounding, and discloses a bactericidal compound composition containing quinolone compounds and application thereof. The composition comprises effective active components, the effective active components comprise a compound I and a compound II, the compound I is 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester, and the compound II is other compounds with bacterial killing activity, and the compound I and the compound II are selected from the group consisting of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester and 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester. The bactericidal compound composition disclosed by the invention has the following advantages: (1) the bactericidal compound composition has a synergistic effect and can improve the prevention and treatment effect; (2) the bactericidal spectrum is expanded, field diseases are mixed, and the effect on bacterial diseases is stronger; (3) the two effective components are mixed for use, so that the drug resistance of pathogenic bacteria can be delayed; and (4) the application amount is reduced, the use frequency is reduced, and the use cost is reduced.

This invention relates to a method for risk assessment of multiple ecological factors in lakes based on DOM (Dissipative Oxygen Species), nutrients, and the coupling of functional bacteria and pathogen resistance. The invention aims to address the technical problems of current lake risk assessment methods, which primarily focus on evaluating single resistance risks, neglect the ecological buffering role of functional bacteria, and fail to reflect the coupled risks of multiple ecological factors in lakes. This invention obtains DOM parameters, nutrient parameters, functional bacteria indicators, pathogenic bacteria indicators, and drug resistance transmission factor indicators; calculates a comprehensive risk value R based on the environmental stress index E, ecological buffering index B, transmission hazard index H, and DOM-mediated transmission triggering coefficient K, and performs risk classification and spatial zoning assessment. This invention incorporates DOM activity, nutrient imbalance, the ecological buffering capacity of functional bacteria, and the drug resistance transmission potential of pathogenic bacteria into a unified evaluation framework, applicable to comprehensive ecological security assessment, risk early warning, and zoning management of lakes such as Poyang Lake.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The utility model relates to the technical field of fire-fighting boots, in particular to a high-comfort fire-fighting protective boot for firefighters, which comprises a boot sole, a toe cap, a vamp, a heel and a boot leg which are integrally arranged, a waterproof sock is jointly attached to the inner surface layers of the toe cap, the vamp and the boot leg, and the boot leg comprises a leg body, a tibia protective layer, an ankle protective layer and an ankle joint bending layer. By means of the arrangement of the waterproof socks on the inner surface layers of the boot vamps and the boot legs, it is guaranteed that when a fireman works in the environments such as wading, the feet cannot be soaked by water, and the waterproof socks further have the functions of high breathability, chemical protection and blood transmission and viral pathogen resistance; meanwhile, the tibia protection layer, the ankle protection layer and the ankle joint bending layer are arranged, and the design fully considers the protection requirements of different parts of the feet of the firefighter when the firefighter executes a task, so that the firefighter can quickly respond in an emergency, and the timeliness and the accuracy of rescue actions are improved.

The present invention relates generally to a plant cell-surface immune receptor engineered to modulate an immune response in a plant, cell or seed, as well as nucleic acid molecules encoding the engineered receptor, and methods of use. In particular, the present invention relates to a plant cell-surface immune receptor into which a coiled-coil (CC) motif is integrated to enhance or modify the plant's immune response, with utility in reducing pathogen infection and improving pathogen resistance.

This invention belongs to the field of microbial technology and discloses a strain of *Citrobacter freundii* bacteriophage PRF337, its bacteriophage composition, and its applications. The *Citrobacter freundii* bacteriophage PRF337 was deposited on May 26, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46489. The bacteriophage PRF337 exhibits broad-spectrum bactericidal activity against *Citrobacter freundii*, capable of lysing *Citrobacter freundii* strains containing different virulence genes and from different sources. It also possesses certain temperature and pH stability and can be used as an active ingredient in pharmaceutical preparations, environmental disinfectants, and water purifiers. It can effectively control *Citrobacter freundii* infection in aquatic animals such as fish and shrimp in aquaculture environments and can also be used for the purification of pathogens in aquatic environments. It is safe to use and effectively avoids the problems of antibiotic residues and pathogen resistance caused by antibiotic use.

The disclosure relates to a plant that is tolerant or resistant to species of Ca. Liberibacter. Specifically exemplified are Citrus and solanaceous plants. Provided by the disclosure is a modified Citrus or solanaceous plant that is resistant or tolerant to Sec-dependent effectors secreted by bacteria. Also provided by the disclosure are methods of modifying a plant genome plant to provide tolerance or resistance to species of Ca. Liberibacter. Still further provided by the disclosure are methods conferring a population of plants with tolerance or resistance to species of Ca. Liberibacter and screening that population for the plants that are tolerant or resistant to species of Ca. Liberibacter.

Synthetic Tombusviridae satellite RNA molecules and satellite particles containing the same are disclosed. Also disclosed are synthetic Tombusviridae satellite RNA molecules containing internal heterologous RNA virus (HRV) amplicons. Methods of using the Tombusviridae satellite RNA molecules and satellite particles containing the same to alter plant phenotype, improve stress resistance of plants, and improve pest and pathogen resistance of plants are also disclosed.

Synthetic turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same are disclosed. Synthetic turnip yellow mosaic virus satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon are also disclosed. Also disclosed are methods of altering plant phenotypes, improving stress resistance in plants, and improving pest and pathogen resistance in plants using turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same.

The application belongs to the technical field of pesticides, and discloses a fungicidal composition and application thereof. The fungicidal composition comprises active ingredient A and active ingredient B. The active ingredient A is a compound of formula I. The active ingredient B is selected from any one of isoflucypram, fluoroimide and fluoroamide. The mass ratio of the active ingredient A to the active ingredient B is 1:35-32:1. The fungicidal composition has significant synergistic effect on various plant diseases in agriculture, is fast-acting, has long persistence, can delay the generation of pathogen resistance, and is suitable for comprehensive management of various plant diseases in agriculture.

The present application belongs to the technical field of fungicides, and particularly relates to a fungicidal composition for preventing and treating rust of figs. The fungicidal composition is used for preventing and treating rust of figs, and is composed of triforine and flusilazole or diclomezine. The present application compounding triforine and flusilazole or diclomezine in a certain mass ratio, and the compounding combination shows a synergistic effect on the rust pathogen of figs, Pucciniastrum ambrosiae, in a certain mass ratio range, and compared with single components, can improve the prevention and treatment effect on the rust of figs, delay the generation and development of the pathogen resistance, and reduce the resistance risk of the fungicidal composition. Secondly, the fungicidal composition can broaden the compounding combination of triforine, and provide support for fully developing the compounding medicament of triforine.

The present application relates to a kind of difficult culture pathogen resistance identification method and its application, belong to the field of agriculture and forestry science, step 1: the culture screening of toxic source plant, plant to be identified and indicator plant;Step 2: grafting transmission, the toxic source plant screened only with difficult culture pathogen, the plant to be identified without difficult culture pathogen and indicator plant are arranged in position, and transmission is carried out using grafting method;Step 3: observation and detection, according to the disease cycle of difficult culture pathogen, the disease phenotype of plant to be identified and indicator plant in step 2 is observed, and the colonization, propagation of difficult culture pathogen is detected by PCR method;Step 4: disease resistance and pathogen running speed evaluation.Effectively solve the problem that the identification result is inaccurate due to the low inoculation rate of difficult culture pathogen using transmission medium, the inconsistency of inoculation source pathogen and other reasons, the error is larger in resistance identification work, etc.

The invention discloses an application of a black spot resistant gene PuSAP3 in plant disease-resistant genetic breeding. The gene PuSAP3 containing the A20 / AN1 type zinc finger transcription factor is separated and cloned from a disease-resistant germplasm, namely pyrus ussuriensis, and the sequence of the gene PuSAP3 is as shown in SEQ ID NO. 1. The gene is used for respectively constructing an over-expression vector and a silent vector, and the over-expression vector and the silent vector are introduced into pear seedlings through an agrobacterium tumefaciens-mediated genetic transformation method to respectively obtain a stably expressed over-expression strain and a TRV-mediated silent strain for disease-resistant function analysis. The result shows that the PuSAP3 transcription factor gene obviously improves the pathogenic bacterium resistance of the plant. Exploration of the gene provides important potential gene resources for plant disease-resistant molecular breeding, provides new insight for disease-resistant breeding of fruit trees and even other crops, and provides assistance for implementation of green agriculture in China.

This invention provides the application of Evodia rutaecarpa root extract in the preparation of antifungal agents, belonging to the field of agricultural fungal control technology. The active ingredient of the Evodia rutaecarpa root extract is naturally derived and easily obtained; its unique chemical structure and novel mechanism of action effectively avoid the problem of pathogen resistance caused by the concentrated mechanisms of action of existing fungicides, filling the market gap for quinolone-based plant-derived pesticides. The Evodia rutaecarpa root extract exhibits a precise antifungal spectrum and significant inhibitory effect, showing obvious inhibitory activity against soil-borne and interplant-borne plant fungi that are serious threats in agricultural production, such as Phytophthora tobaccoii, Fusarium equisetifolium, Colletotrichum gloeosporioides, and Fusarium moniliforme.

Provided is a method for achieving high expression of a target substance in a plant. Also provided are a method of creating a plant with suppressed resistance to a pathogen, wherein the method comprises suppression or disruption of expression of a gene associated with a resistance mechanism in the plant, and a method of creating a plant with suppressed resistance to a pathogen wherein the method comprises overexpression of a gene related to degradation of salicylic acid (SA) in plants; as well as a transgenic plant or genome-edited plant obtained by the method; and a method for producing a target protein using the plant.

Methods and compositions for identifying, selecting and / or producing pathogen resistant plants, plant cells or seeds (e.g., legume or soybean plants, plant cells or seeds) are provided. Polynucleotides and polypeptides are provided that when expressed in a plant increase disease and / or pathogen resistance (e.g., resistance to Asian soybean rust) of the plant. Plants, plant cells, and seeds comprising such polypeptides and polynucleotides are provided, as well as methods of their use.

This invention belongs to the field of genetic engineering technology, specifically relating to the application of the AtHIR4 gene and / or its encoded protein in regulating plant pathogen resistance. The amino acid sequence of the protein encoded by the AtHIR4 gene described in this invention is shown in SEQ ID No. 1. This invention created transgenic Arabidopsis and rapeseed expressing hypersensitivity response-inducible protein 4 (AtHIR4). In the AtHIR4 transgenic Arabidopsis, the chitin-activated ROS burst and phosphorylation of MAPKs were significantly enhanced. Disease resistance test results showed that the AtHIR4 transgenic plants not only exhibited enhanced resistance to the necrotrophic pathogens *Sclerotinia sclerotiorum* and *Botrytis cinerea*, but also significantly increased resistance to the semi-vitrotrophic pathogen *Pseudomonas syringae*. The AtHIR4 transgenic rapeseed also showed significantly enhanced resistance to *Sclerotinia sclerotiorum* and *Botrytis cinerea*.

Synthetic endogenous ribonucleic acid virus satellite RNA molecules and satellite particles containing the same are disclosed. A synthetic endogenous ribonucleic acid virus satellite RNA molecule containing an endogenous heterologous TNA virus (HRV) amplicon is also disclosed. Also disclosed are methods of using endogenous ribonucleic acid virus satellite RNA molecules and satellite particles containing the same to alter plant phenotypes, improve stress resistance in plants, and improve pest and pathogen resistance in plants.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant comprising a quantitative trait locus (QTL) or a causative polymorphism associated with pathogen resistance, and to Cannabis spp. plants having a pathogen resistance trait of interest comprising defined allelic states of polymorphisms defining the QTL. Also provided are plants with a pathogen resistance trait of interest identified by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods for obtaining plants having a pathogen resistance trait of interest, as well as to methods of producing Cannabis spp. plants with the pathogen resistance trait of interest and plants produced by these methods, based on the allelic state of the QTLs or the causative polymorphism.

The invention discloses a feed additive for improving pathogen resistance and intestinal injury of high-fat fed fishes and application of the feed additive, and belongs to the technical field of fish feed. The feed additive is indole-3-propionic acid, and the application of the feed additive is that IPA is added into a high-fat feed according to the addition amount of 20-160 mg / kg. Experiments find that IPA with different concentrations can significantly improve the aeromonas hydrophila infection resistance of tilapia fed with high fat, enhance the intestinal immune enzyme activity, improve the intestinal permeability, enhance the intestinal antioxidant ability and relieve intestinal inflammation. The IPA disclosed by the invention can be used as a novel feed additive, can effectively relieve the problems of pathogen resistance reduction and intestinal injury of high-fat fed fishes, and has a good application prospect.