281 results about "Anthriscus caucalis" patented technology

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention discloses a Bacillius subtilis strain, and the culture preservation code for the Bacillius subtilis at China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.1982. Dry-powder microbial inoculum prepared by the strain can be applied to prevention and cure of wheat scab, rice blast, corn southern leaf blight, sclerotium disease germ of garlic, anthracnose germ of cucumber and other germs that cause diseases to agricultural crops and vegetables; the microbial inoculum can also be applied to preservation of fresh fruits such as peaches, apples, grapes, pears, etc. Rice and corn leaves processed with suspension liquid of Bacillus subtilis strain can prevent the corresponding lesion disease; the cucumber seeds or seedlings processed by mixing the seeds with powder, burying the powder around the root or spraying suspension liquid of Bacillus subtilis strain can not only prevent cucumber anthracnose, but also accelerate the growth of the plants. The production of safe and pollution-free vegetables is capable. Fresh peaches processed with suspension liquid of Bacillus subtilis strain are rot-resistance, and the preservation time is prolonged. The preparation process for the microbial inoculum is simple, applicable to commercial production.

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention relates to a bacillus subtilis preparation for preventing and controlling soil-borne diseases, as well as a preparation method thereof. The bacillus subtilis preparation is prepared by taking the thalli and metabolites of bacillus subtilis B579 with preservation number of CGMCC No.2270 as an effective active constituent which are matched with agriculturally acceptable carrier and a heat-resistant protective agent; the thalli content of the effective active constituent is 8 to 80 billion thalli per gram; and the mass content of the heat-resistant protective agent is 3 to 5 percent. The bacillus subtilis preparation can effectively prevent and control cucumber drooping diseases, cucumber wilt, eggplant root rot, eggplant damping-off, pepper blight, tomato basal stalk rot, grape botrytis cinerea and banana anthracnose. The bacillus subtilis preparation has a control efficiency more than 70 percent, has obvious effect of promoting plant growth, and is characterized in high efficiency, wide antimicrobial spectrum, simple use, low cost, no environmental pollution and improvement on the micro-ecological environment of soil.

An application of the benzothiabiazole as the disease resistance activating agent for preventing and treating the hoja blanca and blast of rice, the powdery mildew of melon, the necrosis host of Nicotiana tabacum L. var. Xanthi-NC, etc is disclosed.

The invention discloses a Hami melon planting method, and belongs to the technical field of melon fruit planting. The method includes the following steps of field preparation, wherein loam or sandy loam facilitating water drainage is selected, a melon filed with the soil pH value of 7-8 is selected; soil preparation and frame erecting, wherein a shed frame is erected in a melon ditch over ridges, base fertilizers are applied to the inside of the melon ditch to form a melon ridge, and holes are dug in the melon ridge; seed selection and sowing, wherein seeds are sown in the holes in the step B after sterilization and insect prevention; planting, wherein when melon seedlings grow to have four true leaves, the melon seedlings are transplanted to the melon ditch in the step C for planting; melon field management, wherein melon vines grow upward along the shed frame, lateral branches of the melon vines are cut timely to guarantee that each melon vine only bears one fruit, and when setting fruits of the melon vines are as big as eggs, the fruits are supported through a string bag; harvest of proper ripe fruits. By means of the method, the problems that cracked melons and rotten melons are prone to being generated during hami melon planting, the melon seedings are prone to being infected by bacteria, gummy stem blight and anthracnose are caused, and quality of the melons is reduced can be solved.

The invention discloses a strain of Paenibacillus jejuni for inhibiting anthrax and Fusarium and an application thereof. A strain of Paenibacillus jamilae is isolated from the deep sea sediment, the strain was deposited at the General Microbial Center of the China Microbial Culture Collection Management Committee under accession number CGMCC No.16231 on August 8, 2018. The strain has broad-spectrum resistance to many plant pathogens, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by Colletotrichum gloeosporioides and vegetable wilt caused by Fusarium oxysporum.

The invention belongs to agricultural use antiseptic field. It discloses N-(2- substituted benzene group) N-methoxy methyl carbamate compound, its preparation and application. The compound has good fungicidal property, can be used to prevent and cure wheat powdery mildew, apple powdery mildew, wheat rust, green smut, corn southern leaf blight, and so on.

The invention relates to a Bacillus methylotrophicus Sanju-04 and its application. The above strain is preserved at China Center for Type Culture Collection (CCTCC) in Wuhan University, has a preservation date of May, 16, 2012, and has a preservation number of CCTCC No:M2012169. The Bacillus methylotrophicus Sanju-04 has very good capsicum bacterial wilt and orange anthracnose control efficacies, and has the advantages of stability, no toxic chemical substance residual, no pollution to agricultural products or the environment, high cost performance, and application convenience. The Bacillus methylotrophicus Sanju-04 plays an important role in the control of the bacterial wilt and the anthracnose of plants, and has a wide application prospect.

The invention discloses a method for preserving litchi by using plant essential oil. The method comprises the following steps: selecting the litchi fruit which is harvested in sunny day, 80%-90% matured, and free from mechanical damage and diseases and insect pests; soaking the litchi fruit in a 25-500 mu L / L of citral or clove leaf oil solution for 0.5-5 minutes; and then precooling, packing andstoring the soaked litchi fruit, wherein after harvesting, the litchi fruit disease mainly comprises fugus diseases such as peronophythora litchi, sour rot, anthracnose and the like, and enzymatic browning of the phenolic compounds and the degradation / discoloration of the anthocyanin are one of the main reasons causing the peel browning. In the method, the litchi fruit which is harvested in proper time and good in state is soaked in the citral or clove leaf oil solution so as to effectively inhibit the fruit rot and the peel browning of the litchi fruit and maintain the quality of the fruit; and in a low temperature condition, the litchi fruit treated by the method disclosed by the invention in completely good condition is more than 95% after 30 days, the control to the peel browning is good, and the original quality of the fruit can be basically maintained.

The invention discloses a Chinese yam cultivation method, which relates to the field of crop cultivation technology. The method includes: variety selection and breeding; soil preparation and plotting: each plot is sloped, the slope of each plot is 10 to 15 degrees, the width of the plot surface is 150cm, and the height of the higher side of the plot surface is 28cm to 31cm; directional trough planting and support erection; vine leading and training, cultivation and banking; fertilizer and water management: additional fertilizer is applied, and water is poured; disease and insect pest control: mainly, stress is laid on the control of soil insects and anthracnose; seedling control; harvesting. Compared with the prior art, the Chinese yam cultivation method utilizes directional troughs to guide the tubers of Chinese yams to transversely grow in the shallow soil layer, thus solving the problem of great harvesting labor intensity.

A control method of grape diseases and insect pests comprises step 1, performing control before germination; step 2, controlling the anthrachose, the gray mold and gall mites during inflorescence phase separation in March; step 3, controlling the anthrachose, the gray mold, the anthracnose and the downy mildew during flowering period in April; step 4, controlling the anthrachose, the white rot, the gray mold, the anthracnose and spider mites during flower and fruit protection and expanding period in May; step 5, performing control during grape bagging in early June; step 6, controlling the anthracnose, the white rot and the downy mildew during fruit coloring and mature period in the middle of June to late June; step 7, giving priority to copper agents and spraying twice after the harvest. The control method of the grape diseases and insect pests is low in cost, free of pollution, high in fruit quality and high in yield.

The invention provides a comprehensive prevention and control method for anthracnose of potted sword-leaved cymbidium, belonging to the field of agricultural plant disease control. By aiming at the four growth stages including a leaf-bud budding stage, a bud and young leave growth stage, a flowering stage and a dormancy recovery stage of the potted sword-leaved cymbidium, five prevention and control measures including agricultural prevention and control, plant quarantine, illness monitoring, biological prevention and control, and chemical agent prevention and control are integrally assembled, so that the comprehensive prevention and control measures suitable for each growth stage are provided. The comprehensive prevention and control method for the anthracnose of the potted sword-leaved cymbidium can efficiently control the anthracnose of the cymbidium, obviously reduces number of use and usage amount of pesticide, and has great significance for improving the ornamental value and the economic value of the potted sword-leaved cymbidium and protecting the ecologic environment.

The invention provides an antagonistic bacterium NZ-4 for wilt of potatoes. The antagonistic bacterium NZ-4 is Bacillus velezensis, with an accession number of CGMCC No. 13606. The invention also provides the application of the antagonistic bacterium NZ-4 to prevention and treatment of soil-borne diseases of crops. Pot experiment results show that the antagonistic bacterium NZ-4 has significant prevention and treatment effect on wilt of potatoes, with prevention and treatment effect being 62.14%. In addition, the strain NZ-4 also has strong antagonistic effect on pathogens leading to anthracnose of scab potatoes, root rot of wheat, etc. The application of the fermentation broth of the antagonistic bacterium NZ-4 can significantly promote the growth of potato plants. The antagonistic bacterium NZ-4 and microbial agents thereof are of great significance for prevention and treatment of the soil-borne diseases and continuous cropping obstacles of farmland in China, the sustainable yield increase of grain and the protection of farmland ecological environment.

The invention provides a natural bactericide for controlling peach tree anthracnose. The natural bactericide is composed of balsam pears, moxa leaves, honeysuckle flowers, anise, small centipeda herb, asarum herb, isatis roots, pogostemon cablin, mangnolia officinalis and persicaria hydropiper. The natural bactericide has rich source of raw materials and is low-cost. The natural bactericide is prepared from pure natural components, nontoxic and nuisanceless. The natural bactericide also can control the peach tree anthracnose effectively.

The invention discloses a method for promoting orchid to grow and preventing diseases, and relates to a method for preventing the diseases and promoting orchid branches to grow and prolonging a flowering phase. The method disclosed by the invention comprises the following steps of uniformly mixing a solid state culture and a culture medium into a basin according to the mass ratio of 1 to 50 in orchid plant transplantation, wherein the solid state culture has the spore concentration s 1*10<7>-1*10<8> spore / g and prepared from Trichoderma harzianum T216, and planting an orchid plant, wherein the solid state culture can induce the resistance of the orchid plant to the fungoid diseases such as southern blight, rhizoctonia solani and anthracnose and bacterial soft rot caused by Erwinia carotovara during orchid growth, the plant morbidity is reduced, the disease index is reduced, the orchid is promoted to grow in advance, and the number of blades of the orchid are increased. In addition, the method disclosed by the invention has the advantages that a liquid state culture of the Trichoderma harzianum T216 is regulated into 1*10<5>-1*10<7> spore / mL when the orchid peeps a bud, and the spray treatment is carried out on the orchid plant, so that the branch number of the orchid is increased, and the flowering phase of the orchid is prolonged.

The invention discloses baciIllus polymyxa KN-03 capable of simultaneously preventing and treating various crop diseases, and the preservation number is CCTCC NO:M2012077. The invention also discloses a culturing method and use of the baciIllus polymyxa. The baciIllus polymyxa can effectively simultaneously prevent and treat crop bacterial wilt, fusarium wilt, verticillium wilt, sclerotinia disease, take-all disease, early blight, gray mold and anthracnose, and has strong sporulation ability, the fermentation liquid spore number can reach 30 billion CFU / ml, at the same time, the baciIllus polymyxa KN-03 also has obvious growth promoting effect, and can significantly increase crop yield.

The invention discloses a pesticide / bactericide for tree daubing. The pesticide / bactericide is prepared from the following components in mass: 1-10% of albendazole, 60-70% of Chinese herbal extract and 20-39% of assistants. A preparation method comprises the following steps of: firstly, mixing the Chinese herbal medicine materials of stemona tubers, celastrus angulatus maxim and melia toosendan according to the mass ratio of 1:1:1, and extracting the mixture with 45% ethanol to obtain an extract for later use; adding the albendazole, the Chinese herbal extract and the assistants according to the formulation into a mixer, and mixing and stirring for 30 min; and treating by a colloid mill, shearing for 50 min, sanding twice, filtering, bottling and packaging to prepare the medicinal product. The pesticide / bactericide has the characteristics of simple and convenient medicine application, easy operation, high medicine utilization ratio and significant medicine application cost and medicine cost. The pesticide / bactericide has obvious effect and long effect acting on aphids, scale insects, red spiders and other pests and anthracnose, canker and other diseases of peach trees, pear trees, waxberry trees, apple trees, orange trees and loquats. The pesticide / bactericide is applicable to tree cultivation.

The invention discloses a disease resistance intensity identifying method for resistance to tobacco anthracnose. The method comprises the following steps: step (I), planting a to-be-identified tobacco variety, a known anthracnose-resistant variety, a moderate anthracnose-resistant variety and a known anthracnose sensing variety in a tobacco field; step (II), transplanting indoors; when true leaves grow on the tobacco seedling, inoculating anthracnose spores; and after the inoculation, growing under the environmental conditions that the temperature is 26-30 DEG C and the relative humidity is over 80% to induce attack; and step (III), detecting the disease resistance intensity of resistance to each variety anthracnose. The invention provides a disease resistance intensity identifying method for resistance to tobacco anthracnose, which can effectively identify the disease resistance intensity of anthracnose to a tobacco variety.

The invention belongs to the technical field of fertilizers of clausena lansium and particularly relates to a compound fertilizer for preventing and treating the anthracnose of clausena lansium trees and a use method of the compound fertilizer. The compound fertilizer comprises the following components in parts by weight: 45-50 parts of straws, 20-35 parts of livestock manure, 1-5 parts of slaked lime, 15-23 parts of urea, 20-24 parts of monoammonium phosphate, 31-36 parts of potassium sulfate, 2-5 parts of borax, 2-4 parts of zinc sulfate, 1-3 parts of magnesium sulfate, 2-6 parts of ferrous sulfate, 5-10 parts of fulvic acid, 3-5 parts of thiophanate methyl wettable powder, 0.2-0.5 part of an EM fungicide and 11-15 parts of a Chinese herbal medicine extracting solution. By utilizing a fertilization manner according to the combination of the growth habit of the clausena lansium trees and the disease period of the anthracnose, the growth of the clausena lansium can be remarkably promoted; and by adding the thiophanate methyl wettable powder, the EM fungicide and the Chinese herbal medicine extracting solution, the anthracnose can be effectively prevented and treated, so that the compound fertilizer has a relatively high promotional value.

The invention belongs to the technical field of cotton planting, and particularly relates to a cotton seedling culture method. The specific method includes the steps of (1) seed selection and treatment, wherein cotton seeds are aired and then put into a rotating machine for rotating treatment, and the cotton seeds are put into a seed soaking agent for soaking and then aired till the moisture is 19%-21%; (2) sowing and after-sowing management, wherein the cotton seeds are sown in a seedling culture medium in a seedling culture shed, light treatment with the light intensity of 44-48 Lx is conducted at 22:00-4:00 every day after emergence, ventilation treatment is conducted at 10:00-15:00 every day after the seedlings send forth one true leaf, and root application of a nutrient solution is carried out 8-9 days before transplanting. By means of the method, the cotton emergence time is shortened to 7.9-8.2 days, the emergence rate reaches 98.41%-98.77%, the blight rate is reduced to 1.45%-1.56%, the anthracnose rate is reduced to 1.23%-1.32%, and the transplanting survival rate reaches 98.5%-98.8%.

The invention discloses a formula and a preparation method of a yeast liquid biocontrol bactericide. The formula comprises yeasts, sucrose, trehalose, galactose, ascorbic acid and a 0.05 mol / L phosphate buffer solution. The method comprises the following steps: activating a yeast strain 4-4A by an NYDB (nutrient yeast dextrose broth) liquid culture medium and inoculating two rings into 50 ml of a seed culture medium; putting a cultured yeast fermentation solution into 50 ml of a centrifugal pipe for conducting a centrifuging process at a speed of 4000 r / min for 15 minutes, dumping supernatant liquor, washing by aseptic water for two times, and adding obtained sediments into the 0.05 mol / L phosphate buffer solution to prepare a bacterial suspension solution; selecting the sucrose, the galactose and the trehalose as protecting agents for preparing a solution by the phosphate buffer solution; adding 21.6 ml of the solution into 2.4 ml of a yeast mother solution with 109 CFU / ml (colony forming unit / ml) of the yeasts, fully and uniformly mixing, and sub-packaging a mixture into 1.5 ml of the centrifugal pipe; measuring the number of living yeasts in a yeast preparation. According to the yeast liquid biocontrol bactericide, the occurrence rate of anthracnose after mangoes are harvested is effectively reduced, the yeast liquid biocontrol bactericide is applied for 4-6 times from an early flowering period to a harvest period to prevent and control mango anthracnose, the morbidity and the severity are reduced by 64%-67% and 89%-90% respectively, and the disease prevention and control effect is improved.

The invention discloses a preparation method of botanical pesticide for preventing cotton anthracnose, which comprises tea seed cakes, rhizoma coptidis, tobacco, pagoda tree leaves, bead leaves, gingers and one-clove garlic. The botanical pesticide has the advantages that the formulation and the preparation method are simple, the cost of raw material is low, the compatibility is simple, the prevention effect is good, and various beneficial microorganism and organic fertilizer contained in the botanical pesticide can also achieve the effect of fertilizer increasing, promote cotton to be full and have heavy weight. The botanical pesticide not only has the effect of increasing the output, but also effectively solves the defect that the residual pesticide ingredient in cotton is too high.

The invention discloses a degraded soil improving method with stylosanthes guianensis as ecological forage. The JinshaRiver dry-hot valley degraded soil is chosen to be treated with the method. According to the method, thoroughly decomposed farmyard manure and calcium superphosphate are applied during soil preparation, stylosanthes guianensis seedlings are transplanted in the rainy season, urea is further applied for one to two times when the stylosanthes guianensis seedlings germinate, timely drainage is guaranteed in the rainy season and the soil water content is limited below 15%, timely watering is guaranteed in the arid season and the soil water content is kept above 3%, 50% carbendazim wettable powder is used every year to prevent the occurrence of anthracnose, and 20% acetamiprid powder 3000 times liquid is used to kill aphids or armyworms in a spraying mode. According to the ecological characteristic of the Jinsha River dry-hot valley degraded soil, the method fully utilizes characteristics, such as drought-enduring, growing fast, cutting-enduring, strong nitrogen fixing capacity, strong adaptability and extensive management, of the stylosanthes guianensis, soil organic matter, the total nitrogen and available phosphorus content, and soil water contents in soil layers of 0-20 cm and 20-40 cm in the arid season are greatly improved.

The invention discloses a plant source pesticide for preventing and treating apple tree rot and belongs to the technical field of prevention and treatment of fruit tree diseases and insect pests. The pesticide comprises the following raw materials: Chinese prickly ash, garlic, aloe, clove, radix isatidis, an antifreezing agent, a wound protective agent and an anti-corrosive agent. The plant source pesticide is simple in prescription and process, as long as two years in preservation period, low in cost and good in prevention and treatment effect, has the characteristics of being free of residues, free of drug resistance (tolerance) and free of pollution, has a remarkable prevention and treatment effect on the apple tree rot, is above 95% in prevention rate, also has a certain prevention and treatment effect on fungous diseases such as anthracnose, ring spot, powdery mildew and dark spot which are harmful to apple trees, and also can be used for quickening healing of scab wound of the apple trees while preventing and treating the apple tree rot.

The invention discloses a planting method for dendrobium officinale. The planting method includes the following steps that firstly, a seedling growing foundation is prepared; secondly, a seedling pot is prepared; thirdly, field planting is conducted; fourthly, later-period management is conducted, wherein the later-period management includes watering, fertilizing and disease and pest prevention; fifthly, harvesting is conducted. According to disease and pest prevention, an isolation ditch is formed in the periphery of a seedbed for surrounding the seedbed, the width of the isolation ditch is 30-50 cm, and the isolation ditch is filled with water or oil; carbendazim diluted 1000-fold liquid with the volume concentration of 50 percent is used for the dendrobium black spot disease happening from March to May, and spraying is conducted 2-3 times a month; carbendazim diluted 1000-fold liquid with the volume concentration of 50 percent or thiophanate methyl 1000-fold liquid with the volume concentration of 50 percent is used for the dendrobium anthracnose happening from January to May, and spraying is conducted 2-3 times a month. According to the planting method for the dendrobium officinale, the survival rate of the dendrobium officinale is increased, the content of various nutrition components, dendrobine, polysaccharide and coarse fibers of the dendrobium officinale planted through the method is high, in addition, diseases and pests in the planting process are obviously reduced, the use amount of pesticide is reduced, and the edible safety of the dendrobium officinale is improved.

The invention discloses a substituted five-membered heterocyclic compound containing pyrimidine rings. The structure is shown in a formula I: (the formula I is shown in the description), and the definition of each substation group is shown in the description. The substituted six-membered heterocyclic compound has the advantages that the broad-spectrum bacteria-killing and insect-killing activity is realized; the good preventing and controlling effect is realized for cucumber downy mildew, wheat powdery mildew, puccinia polysora, rice blast, cucumber anthracnose and the like.