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116 results about "Colony-forming unit" patented technology

In microbiology, a colony-forming unit (CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells in a sample. Viable is defined as the ability to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all cells, living or dead. The visual appearance of a colony in a cell culture requires significant growth, and when counting colonies it is uncertain if the colony arose from one cell or a group of cells. Expressing results as colony-forming units reflects this uncertainty.

Probiotic food, process for its preparation and dietary regimen

InactiveUS20070160589A1Easy maintenanceStrong feelingBiocideMetabolism disorderWater activityDietary regimen
A probiotic food item containing a beneficial amount of dry active probiotic cultures is provided. The food item also contains a substantial continuous fat-based coating with an effectively low water activity level. The food item is packaged in a substantially moisture impermeable package marked with a use by or sell by date, so as to ensure a desired minimal amount of probiotic colony forming units (CFUs) on the use by or sell by date. Methods for manufacturing the probiotic food product are also provided.
Owner:ATTUNE FOODS

Novel compound microorganism living bacteria agent, method for preparing same and applications

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu) / gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.
Owner:SHANGHAI ECO WELL BIOSCI

Microbial deodorant and preparation method thereof

InactiveCN102258796AStrong fermentation and decomposition abilityGrowth inhibitionFungiBacteriaCfu - colony-forming unitSaccharomyces cerevisiae
The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit) / ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.
Owner:黄小娃

Method of determining soybean sudden death syndrome resistance in a soybean plant

InactiveUS7288386B2Solves the problem quickly and cheaply selecting resistant cultivarsImprove selection for SDS and SCN resistanceMicrobiological testing/measurementFermentationCell culture mediaBacterial growth
A method of determining the presence of soybean sudden death syndrome resistance in the soybean plant in a greenhouse setting, the method comprising the steps of: (a) inoculating soil with a low density inoculum of Fusarium solani; (b) planting a soybean plant in said inoculated soil; (c) growing said plant in said soil in a greenhouse; (d) isolating Fusarium solani-infected tissue from said plant; (e) culturing said infected tissue for a period of time sufficient to allow for fungal colony forming unit growth; (f) scoring at least one of disease severity and infection severity in said plant using the number of said fungal colony forming units; and (g) correlating at least one of said disease severity and said infection severity to at least one of disease severity and infection severity data from genetic markers associated with soybean sudden death syndrome resistance to identify a correlation, wherein a statistically significant correlation indicates presence of soybean sudden death syndrome resistance in said soybean plant. Also provided is a method of characterizing resistance to soybean sudden death syndrome in a soybean plant, the method comprising the steps of: (a) isolating roots from a soybean plant infected by Fusariurn solani; (b) culturing the root on a culture plate including a restrictive growth medium that provides for slow fungal growth and restricted bacterial growth; (c) determining root infection severity by statistically evaluating the number of Fusarium solani colony forming units on said culture plate; and (d) characterizing resistance to soybean sudden death syndrome in said soybean plant based on said determined root infection severity.
Owner:SOUTHERN ILLINOIS UNIVERSITY

Biofertilizer for improving saline and alkaline lands and preparation method and application of biofertilizer

The invention relates to biofertilizer for improving saline and alkaline lands. The biofertilizer comprises the following components in parts by weight: 200 to 250 parts of fermented mushroom residue, 150 to 200 parts of ammonium sulfate, 50 to 100 parts of superphosphate, 50 to 100 parts of complex improver, 15 to 21 parts of EDTA (ethylene diamine tetraacetic acid)-metallo-chelate, 0.5 to 1 parts of ammonium molybdate and 5 to 10 parts of borax, and also comprises 20 to 30 parts of compound micro-organism, so that the living bacteria count of the biofertilizer is not less than 5.0 * 108CFU (colony-forming unit) per gram. The invention also relates to a preparation method for the biofertilizer and the application of the biofertilizer in crop planting. The biofertilizer for improving the saline and alkaline lands, disclosed by the invention, has important functions of improving the soil conditions of the saline and alkaline lands, improving the soil fertility, increasing crop yield and preventing the soil from secondary salinization.
Owner:烟台瑞腾生物技术研究院有限公司

Method for recovering cadmium polluted soil by combining compound microorganism bacterium agent with houttuynia cordata

InactiveCN103071672AImprove the effect of bioremediationImprove repair effectContaminated soil reclamationHouttuyniaCombined method
The invention discloses a method for recovering cadmium polluted soil by combining a compound microorganism bacterium agent with houttuynia cordata. The compound microorganism bacterium agent comprises the following components by count: 1*10<9>-9*10<9>CFU (colony forming unit) / m<3> pseudomonas fluorescens, 1*10<8>-6*10<8>CFU / m<3> actinomyces ruminicola, and 1*10<9>-9*10<9>CFU / m<3> glomus mosseae. The method for recovering the soil comprises the following steps that the houttuynia cordata is planted in cadmium soil and the compound microorganism bacterium agent is inoculated into the soil, or the houttuynia cordata is soaked in the compound microorganism bacterium agent and then planted into the cadmium soil; when branches and leaves of the houttuynia cordata are luxuriant, the branches and the leaves of the houttuynia cordata are harvested; and cadmium in the soil can be removed. The compound microorganism bacterium agent can better improve the tolerance of the houttuynia cordata, promote growth of a plant in the high-cadmium-content soil, improve the absorbility of the plant to cadmium, and enhance a soil heavy metal removal effect. The method for recovering the soil is a safe, environment-friendly, high-efficiency, economical and practical plant-microorganism combined method for recovering the heavy metal polluted soil, and has a significant application value.
Owner:SICHUAN UNIV

Additive for predigestion fermented feed for piglet

The invention discloses an additive for predigestion fermented feed for piglets, relating to pannage additive. The additive for predigestion fermented feed for piglets can stably improve the digestibility of feed and maintain the microecological balance of intestinal tracts. Each ton of perfect compound feed comprises 800-1000g of complex enzyme preparation, 300-500g of probiotic bacteria and 3000-5000g of lactic acid bacteria liquid, wherein 1g of complex enzyme preparation contains 800-1200U of acid protease, 80-120U of amylase, 1800-2200U of saccharifying enzyme, 12000-18000U of xylanase, 4000-5000U of beta-glucanase, 12000-16000U of cellulose, 6000-9000U of pectinase and 400-600U of phytase; 1g of probiotic bacteria contains 1.0*1010-2.0*1010CFU (colony-forming unit) of Bacillus licheniforms endogenous spore and 1.0*1010-2.0*1010CFU of yeast cell; and 1ml of lactic acid bacteria liquid contains 1.0*109-2.0*109CFU of lactic acid bacteria.
Owner:沈阳爱特杰牧业有限公司

Microbial inoculum for degrading straw

The invention discloses a microbial inoculum for degrading straw and the active ingredients of the microbial inoculum comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratio of Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The microbial inoculumfor degrading straw of the invention can mature straw fast to prepare organic fertilizer, and the prepared organic fertilizer can be uses as a base fertilizer and can obviously improve the quality ofvegetables.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Straw organic fertilizer and preparation method thereof

The invention discloses a straw organic fertilizer and a preparation method thereof. The preparation method is to use a microbial inoculum used for degrading straw to ferment straw so as to obtain thefertilizer; the active ingredients of the microbial inoculum for degrading straw comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratioof Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The fertilizer used for degrading straw can be used as a base fertilizer and can obviously improve the quality of vegetables.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Production method of microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes

The invention discloses a production method of a microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The production method is characterized by comprising the following steps: carrying out symbiotic assemblage screening on lactic acid bacteria and saccharomycetes separated from an Inner Mongolia dairy product kumiss to select a set of enterococcus faecium HE5 and brewer yeast J-4 with mutual promotion, wherein the viable count of the lactic acid bacteria reaches 2.88*10<9>CFU (Colony-Forming Unit) / mL when enterococcus faecium HE5 and brewer yeast J-4 screened are cultivated on an optimized skimmed milk culture medium, the viable count of the saccharomycetes reaches 3.3*10<8>CFU / mL, and double bacteria culture is better to promote the growing effect of lactic acid bacteria; and freezing and drying the cultivated and harvested double bacteria to obtain the microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The microbial preparation is good in symbiotic effect and strong in acid-producing capacity.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Straw in-situ decomposition microbial agent and application thereof

The invention discloses a straw in-situ decomposition microbial agent. Active ingredients of the microbial agent are formed by mixing clostridium xylanolyticum, xylan monad cellulolyticus, pseudomonas and bacillus subtilis in a CFU (Colony Forming Unit) ratio of (25-35): (25-30):(20-30):(15-20). Experiments prove that the microbial agent provided by the invention has degradation rate for straws, which can reach 70%-80% in a culture medium, and can reach over 50% when the straws are returned to the field for 20 days. The microbial agent disclosed by the invention is low in cost and simple to prepare. The microbial agent disclosed by the invention breaks through the limit that natural cellulose cannot be efficiently decomposed by purifying bacteria and clastic enzymes thereof, provides a key technology for straw in-situ rapid decomposition, and has a wide application prospect in the field of returning straws to the field and decomposing a lignocelluloses material.
Owner:MOUNT EMEI GREEN LAND ECOLOGICAL AGRI DEV LIMITED

Silage inoculant and application thereof

The invention relates to a silage inoculant and a preparation method and application of the silage inoculant. The silage inoculant is prepared by respectively fermenting lactobacillus plantarum, bacillus subtilis and lactococcus and mixing the obtained fermentation broth, wherein the ratio of numbers of colony-forming units of lactobacillus plantarum to bacillus subtilis to lactococcus lactis in the mixed fermentation broths is (90-96) to (3.5-6) to (0.5-4), and the total number of colony-forming units is 0.8*10<12>-1.2*10<12>CFU / ml. Various lactic acid bacteria in the fungicide have a high synergistic effect, and form a stable eco-system, and non-sterile natural raw materials can be quickly propagated and colonized to hold advantages, so that large amounts of lactic acid are quickly generated, the pH value of fermented materials is reduced, the fermentation quality of a fodder is improved, the nutritional value and the palatability of the fodder are improved, the application is simple and easy, and the cost is low.
Owner:CHINA AGRI UNIV

Bushi lactobacillus prepn., prepn. method and use thereof

The invention discloses a Bu lactobacillus preparation that contains 1*109-1*1011 Bu lactobacillus colony forming unit. It also discloses the manufacture method and the usage. It could used to improve the quality of corn silage.
Owner:SOUTH CHINA AGRI UNIV

Method for culturing hematopoietic stem/progenitor cells

InactiveCN101597594AA large amountGood ability to rebuild bloodTissue cultureProgenitorBiotechnology
The invention belongs to the field of biotechnology, and relates to a method for expanding the hematopoietic stem / progenitor cells from umbilical cord blood in vitro by an alginate three-dimensional culture system. The method combines SCF, TPO and FL cytokines to support the expansion of the hematopoietic stem / progenitor cells in a single karyocyte of human umbilical cord blood, does not need adding other cytokines, and cultures the single karyocyte of human umbilical cord blood packaged in an alginate microsphere in a three-dimensional stationary culture system and a three-dimensional rotary culture system to observe the expansion effect of the single karyocyte of human umbilical cord blood packaged in the alginate microsphere in the rotary culture system. Compared with the conventional two-dimensional system, the three-dimensional culture system improves the total cells and obviously increases CD34+ cells under the condition of culturing low-concentration cytokines; and colony forming unit-granulocytes and macrophages of cells expanded in the three-dimensional culture system are cloned to form obviously increased cells, and the single karyocyte of human umbilical cord blood in a transplanted NOD / SCID mouse has better engraftment and reconstructs hemopoiesis.
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV

Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis

The invention relates to the field of microorganism application, in particular to Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparations and application of the Bacillus licheniformis. The invention provides Bacillus licheniformis CRVAB001 for producing lactic acid at high yield after fermentation in the collection number of CGMCC No.4850. The inventionalso provides a microecological liquid preparation comprising the Bacillus licheniformis, a preparation method for the microecological liquid preparation, microecological powder comprising the Bacillus licheniformis, and a preparation method for the microecological powder. The Bacillus licheniformis can be efficiently and quickly fermented to produce the lactic acid, and has great industrial application prospect. The viable content of the liquid preparation is more than 1*10<10> colony-forming units (CFU) / mL, the viable content of the powder is more than 1*10<11>CFU / g, and the effectiveness of the Bacillus licheniformis preparations is fully guaranteed.
Owner:北京科为博生物科技有限公司

High pressure processing of foods

High pressure processing (HPP) uses isostatic pressure to evenly apply great pressures to foods on all sides of the foods. Because the pressure is isostatic, the food is not deformed, so long as there is no internal cavity in the food. The pressures used are preferably from about 100 MPa (about 14.5 ksi) to about 1000 MPa (about 145 ksi), and the temperature rise is preferably limited to about 3° C. (about 5° F.) per 100 MPa (about 14.5 ksi). The high pressure dramatically reduces pathogens in the food so processed, as much as a four or five-log reduction in counts of colony forming units (CFU). The process has been shown to be effective in achieving these reductions in E. Coli, listeria, and salmonella. Pieces or sticks of beef jerky or sausage may be processed via HPP and combined with cheese to form a food product with a long shelf life.
Owner:SNACK PATROL

Polycyclic aromatic hydrocarbon antibacterial agent of composite carrier and preparation method of polycyclic aromatic hydrocarbon antibacterial agent

The invention discloses a polycyclic aromatic hydrocarbon antibacterial agent of a composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent comprises a Mycobacterium vanbaalenii. PYR-1 bacterium suspension and a carrier, wherein the volume / mass ratio of the bacterium suspension to the carrier is (20-50)ml: 15g; (5.0-6.0)*10<8>CFU (Colony-Forming Unit) of the Mycobacterium vanbaalenii. PYR-1 thallus is contained in the bacterium suspension per ml; the carrier comprises black charcoal and humus in the mass ratio of (1.8-2.2):1. The invention also discloses a preparation method of the polycyclic aromatic hydrocarbon antibacterial agent of the composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent is capable of effectively reducing the content of the polycyclic aromatic hydrocarbon in the polluted environment.
Owner:ZHEJIANG UNIV

Bacterial cultivation system for growth of substrate specific micro-organisms for use in industrial wastewater remediation

A waste treatment method includes the concentration of selected strains of bacteria in a selected medium in the presence of nutrients and water, under aerobic conditions. This concentrated batch is thereafter discharged for downstream applications in wastewater remediation. The present invention employs a cultivation chamber having inlet ports and a circular vent port that allows for adequate air introduction and heat release. Aeration is achieved by recirculation of the fluid medium from the top of the apparatus through a pipe that runs the length of the inner wall and is specially configured at the top to minimize cell damage. Fluid can be routed tangentially in both the clockwise and counterclockwise directions within the chamber. The conical bottom also has an orifice allowing for recirculation of the fluid medium tangentially to the sidewalls. Upon completion of the batch cultivation, the medium and bacteria are discharged for downstream applications in wastewater remediation of paper mill, chemical plant, oil refinery, and other industrial effluents. The aeration and circulation of the fluids is designed to limit cells shearing and damage yet achieve critical cell mass (e.g. between 107 and 1010 cfu (colony forming units) per milliliter (ml)) and dissolved oxygen uptake rates (e.g. between about 50 and 1500) during cultivation.
Owner:ENVIRONMENTAL BUSINESS SPECIALISTS

Formula and preparation method of yeast liquid biocontrol bactericide

The invention discloses a formula and a preparation method of a yeast liquid biocontrol bactericide. The formula comprises yeasts, sucrose, trehalose, galactose, ascorbic acid and a 0.05 mol / L phosphate buffer solution. The method comprises the following steps: activating a yeast strain 4-4A by an NYDB (nutrient yeast dextrose broth) liquid culture medium and inoculating two rings into 50 ml of a seed culture medium; putting a cultured yeast fermentation solution into 50 ml of a centrifugal pipe for conducting a centrifuging process at a speed of 4000 r / min for 15 minutes, dumping supernatant liquor, washing by aseptic water for two times, and adding obtained sediments into the 0.05 mol / L phosphate buffer solution to prepare a bacterial suspension solution; selecting the sucrose, the galactose and the trehalose as protecting agents for preparing a solution by the phosphate buffer solution; adding 21.6 ml of the solution into 2.4 ml of a yeast mother solution with 109 CFU / ml (colony forming unit / ml) of the yeasts, fully and uniformly mixing, and sub-packaging a mixture into 1.5 ml of the centrifugal pipe; measuring the number of living yeasts in a yeast preparation. According to the yeast liquid biocontrol bactericide, the occurrence rate of anthracnose after mangoes are harvested is effectively reduced, the yeast liquid biocontrol bactericide is applied for 4-6 times from an early flowering period to a harvest period to prevent and control mango anthracnose, the morbidity and the severity are reduced by 64%-67% and 89%-90% respectively, and the disease prevention and control effect is improved.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI

Stabilizing methods for coating seeds with biological materials

The present invention provides a method for coating seeds with biological materials such as bacteria, fungi (e.g., yeasts and molds), parasites, recombinant vectors, and viruses. The method comprises (a) applying a moistening liquid to seeds to moisten seeds, wherein the moistening liquid comprises a moistening polymer, and (b) coating the moistened seeds with an effective amount of a dry composition, wherein the dry composition comprises biological materials, one or more disaccharides, one or more oligosaccharides, one or more polysaccharides, one or more carboxylic acid salts, and one or more hydrolyzed proteins. The coated seeds may have an initial water activity (Aw) below 0.4, and the microorganisms on the coated seeds have initial viability of at least 5 logs of colony forming units per gram of seeds (CFU / g seed). Also provided are coated seeds.
Owner:ADVANCED BIONUTRITION CORP

Pleurotus eryngii yoghurt and production method thereof

InactiveCN103444869AIncrease health functionPromoting rational processing and utilizationMilk preparationAdditive ingredientProduct processing
The invention provides pleurotus eryngii yoghurt and a production method thereof, and belongs to the technical field of food science. The pleurotus eryngii yoghurt is milk white in color, has uniform and exquisite texture, moderate viscosity, faint fragrance of pleurotus eryngii and special aroma of fermented milk, and moderate soursweet, and contains not less than 3.0% of protein, not less than 3.1% of fat, not greater than 70 thorner degrees of acidity, and not less than 1*10<6>CFU (Colony Forming Unit) / mL of lactobacilli count. The production method comprises the following steps: getting pleurotus eryngii and milk as the major raw materials; slicing and pre-cooking the pleurotus eryngii; pulping the pleurotus eryngii with equivalent water; adding milk, granulated sugar, a stabilizer and a sweetening agent in proportion; homogenizing; sterilizing; cooling; adding 2.5% of lactobacillus bulgaricus and streptococcus thermophilus in proportion of 1: 1.5; filling; fermenting at 42 DEG C until the titration acidity reaches 65 to 75 thorner degrees; and stopping to refrigerate and after-ripen for 24 hours at 4 to 6 DEG C to obtain the pleurotus eryngii yoghurt. The production method has the beneficial effects that the whole pleurotus eryngii is pulped and added, thus the variety and content of nutritional components in the yoghurt are increased, the healthcare function of the yoghurt is improved, and new taste is created. The production method is important for promoting the rational processing and utilizing of pleurotus eryngii resources, formation of an after-production processing industry chain and adjustment of an agriculture product processing industry structure.
Owner:HEBEI AGRICULTURAL UNIV.

Additive having function of regulating intestines and stomach of pet as well as preparation method and application thereof

The invention provides an additive having a function of regulating intestines and stomach of a pet as well as preparation method and application thereof, and belongs to the technical field of animal feed. The additive having the function of regulating the intestines and the stomach of the pet is prepared from the following components in parts by weight: 2 to 10 parts of lactobacillus acidophilus powder, 1 to 5 parts of fructus crataegi, 2 to 10 parts of rhizoma dioscoreae, 2 to 10 parts of poria cocos, 1 to 5 parts of liquorice root and 1 to 5 parts of pericarpium citri reticulatae, wherein the viable bacteria concentration of the lactobacillus acidophilus powder is 0.5*10<10> to 1.5*10<10> CFU (Colony Forming Unit) / g. The additive provided by the invention is capable of obviously increasing anti-stress capability of puppies in a weaning stage and regulating the gastrointestinal function, an experiment shows that compared with a control group, the prevalence rate, the appetite, the skin and hair glossiness, the activity, the daily gain and the intestinal flora composition of an experimental group are all remarkably improved, and compared with a rhizoma dioscoreae and poria cocos taste-lack group, the prevalence rate, the appetite, the skin and hair glossiness, the activity and the daily gain of the experimental group have remarkable difference.
Owner:JILIN UNIV

Ripening fungicide for corn stalk substrate generation and application

The invention provides a ripening fungicide for corn stalk substrate generation. Active ingredients of the ripening fungicide are clostridium thermocellum, xylanimonas cellulosilytica, bacillus cereus and bacillus licheniformis. The ratio of colony forming units of the four fungi is (4-5):(2-3):(2-3):(2-3), and the effective viable organism number of the fungicide is 109 / mL or above. The invention further provides application of the ripening fungicide to decomposing fermentation for corn stalk substrate generation. The ripening fungicide has a high corn stalk degradation rate, 40-70% of corn stalks can be decomposed within 20 days during large-scale ripening fermentation, an important technical means is provided for corn stalk substrate generation, and the ripening fungicide has broad application prospects in the field of organic culture substrate production.
Owner:大庆市春田绿土农业科技有限公司

Methods for co-culturing cord blood derived cells with menstrual stem cells

Methods are provided for obtaining expanded human cord blood cells expressing CD34. The methods involve seeding a sufficient amount of cord blood cells with a sufficient amount of menstrual cells under co-culture conditions suitable to promote expansion of the cord blood cells, and co-culturing the cord blood cells with the menstrual cells under culture conditions that support at least two or more population doublings of the cord blood cells. Methods are also provided for growing expanded human cord blood cells to give rise to any one of colony forming units, colony forming unit granulocyte macrophages (CFU-GM), burst forming unit erythroids (BFU-E), and colony forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU-GEMM) blood lineage precursor cells. The expanded cells may express CD34, SSEA-4, and HLA-II. Compositions of the expanded cells are also provided.
Owner:CRYO CELL INTERNATIONAL INC

C. novyi for the treatment of solid tumors in humans

The present invention provides, inter alia, methods for treating or ameliorating an effect of a solid tumor present in a human. These methods include administering intratumorally to the human a unit dose of C. novyi, preferably C. novyi NT, colony forming units (CFUs), which contains about 1×103-1×107 CFUs suspended in a pharmaceutically acceptable carrier or solution. Methods for debulking a solid tumor present in a human, methods for ablating a solid tumor present in a human, a method for microscopically precise excision of tumor cells in a human, methods for treating or ameliorating an effect of a solid tumor that has metastasized to one or more sites in a human, unit doses of C. novyi, preferably C. novyi NT, CFUs, and kits for treating or ameliorating an effect of a solid tumor present in a human are also provided.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE +1

Microbial powder, infant yoghourt and preparation method thereof

The invention discloses a microbial powder, an infant yoghourt and a preparation method thereof. The microbial powder comprises a compound microbial powder of streptococcus thermophilus STI-13 and streptococcus thermophilus Body-3 at a ratio of 1:1 to 1:4 by colony forming unit quantity. The infant yoghourt obtained by fermentation by using the microbial powder is free of D-lactic acid, and the protein component, the fat structure component and the fatty acid component are all similar to those of breast milk, therefore the infant yoghourt can be favorably digested and assimilated by infants.
Owner:BRIGHT DAIRY & FOOD

Vibrio parahaemolyticus standard substance containing fish substrate

The invention belongs to the technical field of food microorganisms, and particularly relates to a vibrio parahaemolyticus standard substance containing fish substrates. Concretely, a finished productwith the bacterium number being 100 to 350 CFU (colony forming unit) per bottle is prepared through storage liquid preparation, substrate pretreatment, suspension preparation, standard substance preparation, strain identification, uniformity verification, constant value obtaining and stability inspection. The obtained vibrio parahaemolyticus standard substance containing fish substrates has gooduniformity; the problem that the fish substrates in the existing standard substance are difficult to dissolve in water can be solved; the quality is stable; the quality is controllable; the vibrio parahaemolyticus standard substance is applicable to efficient and accurate quantitative detection of microorganisms.
Owner:INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Probiotic fermentation beverage capable of preventing and controlling diabetes mellitus, and preparation method thereof

InactiveCN110558463AUnique flavorHigh content of active lactic acid bacteriaFood ingredient functionsEffect functionCulture fungus
The invention discloses a probiotic fermentation beverage capable of preventing and controlling diabetes mellitus, and a preparation method thereof, and belongs to the technical field of fermentationbeverage preparation. In order to solve the problem that personalized eating is difficult in realization since an existing health care type probiotic fermentation beverages has a complex fermentationtechnology, the invention utilizes optimal lactic acid bacteria cultures suitable for fruit and vegetable fermentation and functional cultures capable of lowering blood glucose, and a fermentation accelerant is taken as an auxiliary material to prepare a lactic acid bacteria fermentation bacteria preparation. Under a family environment, the active lactic acid bacteria content of the probiotic fresh brewing fermentation beverage which has a low carbohydrate content, is proper in sour and sweet taste and has a special flavor is prepared through anaerobic fermentation for 60-96h at a constant fermentation temperature of 35-38DEG C through a standard operation specification for homemade fresh brewing fermentation beverages is as high as 2.9*10<10>CFU (colony-forming unit) / 100mL. The product disclosed by the invention is high in safety, does not have a side and effect function, and has a good application value and market prospect.
Owner:哈尔滨酵养集生物技术有限公司

Prawn feed containing rhodobacter capsulatus strain and application thereof

The invention discloses prawn feed containing rhodobacter capsulatus strain. The prawn feed containing rhodobacter capsulatus strain is characterized by being prepared from prawn feed, rhodobacter capsulatus strain fermentation liquor and sodium alginate, wherein 10g of odium alginate and 20mL of rhodobacter capsulatus strain fermentation liquor are added into each 100g of prawn feed until concentration is 3-6*107CFU (Colony Forming Unit) / g; the rhodobacter capsulatus strain is a (i) Rhodobacter capsulatus( / i) R3 strain and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No:11753. The rhodobacter capsulatus is used as a feed additive to manufacture the prawn edible feed, a feeding result shows that the growth speed of the prawn which eats the feed in which the rhodobacter capsulatus is added is greatly improved compared with the prawn which eats common feed, and immunity is obviously improved. Since the rhodobacter capsulatus is used for manufacturing the feed, the production period is short, the production cost is low, a production condition can be easily controlled, the antimicrobial activity is high, the immunity can be obviously enhanced and the like, and the prawn feed has the potential of industrial production and has a good production application prospect.
Owner:TIANJIN NORMAL UNIVERSITY

Application of resveratrol in preparing medicament for preventing and treating radiation induced depigmentation skin disease

The invention discloses application of resveratrol in preparing a medicament for preventing and treating radiation induced depigmentation skin disease. A path for treating occurrence and development of ionizing radiation induced leucoderma is established by treating a radiation induced IRM-2 mouse leucoderma model with resveratrol. Mice irradiated by 6Gy after 30 days orally take the medicament for 15 days, and then the result shows that the depigmentation inhibiting rate of the medicament administration group reaches 40% compared with the control group, so the medicament has remarkable effect. Pathological detection results show that hair follicles of the pathological change part of the mice of the resveratrol administration group are distributed more densely, and the distribution of melanin is obviously wider than that of an irradiation group and a normal group. The immune indexes of the mice of the resveratrol administration group, such as peripheral blood leukocyte number, femoral nucleated cell number, CFU-S (colony forming unit-spleen), CFU-GM (colony forming unit-granulocyte and monocyte) and the like, are slightly higher than those of the irradiation group, so the resveratrol has certain protection effect on hematopoiesis and immunologic function of the irradiated mice. The results show that the resveratrol has positive prevention and treatment effect on the occurrence and development of the radiation induced mice leucoderma.
Owner:INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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