116 results about "Colony-forming unit" patented technology

A method of determining the presence of soybean sudden death syndrome resistance in the soybean plant in a greenhouse setting, the method comprising the steps of: (a) inoculating soil with a low density inoculum of Fusarium solani; (b) planting a soybean plant in said inoculated soil; (c) growing said plant in said soil in a greenhouse; (d) isolating Fusarium solani-infected tissue from said plant; (e) culturing said infected tissue for a period of time sufficient to allow for fungal colony forming unit growth; (f) scoring at least one of disease severity and infection severity in said plant using the number of said fungal colony forming units; and (g) correlating at least one of said disease severity and said infection severity to at least one of disease severity and infection severity data from genetic markers associated with soybean sudden death syndrome resistance to identify a correlation, wherein a statistically significant correlation indicates presence of soybean sudden death syndrome resistance in said soybean plant. Also provided is a method of characterizing resistance to soybean sudden death syndrome in a soybean plant, the method comprising the steps of: (a) isolating roots from a soybean plant infected by Fusariurn solani; (b) culturing the root on a culture plate including a restrictive growth medium that provides for slow fungal growth and restricted bacterial growth; (c) determining root infection severity by statistically evaluating the number of Fusarium solani colony forming units on said culture plate; and (d) characterizing resistance to soybean sudden death syndrome in said soybean plant based on said determined root infection severity.

The invention discloses a straw organic fertilizer and a preparation method thereof. The preparation method is to use a microbial inoculum used for degrading straw to ferment straw so as to obtain thefertilizer; the active ingredients of the microbial inoculum for degrading straw comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratioof Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The fertilizer used for degrading straw can be used as a base fertilizer and can obviously improve the quality of vegetables.

The invention discloses a straw in-situ decomposition microbial agent. Active ingredients of the microbial agent are formed by mixing clostridium xylanolyticum, xylan monad cellulolyticus, pseudomonas and bacillus subtilis in a CFU (Colony Forming Unit) ratio of (25-35): (25-30):(20-30):(15-20). Experiments prove that the microbial agent provided by the invention has degradation rate for straws, which can reach 70%-80% in a culture medium, and can reach over 50% when the straws are returned to the field for 20 days. The microbial agent disclosed by the invention is low in cost and simple to prepare. The microbial agent disclosed by the invention breaks through the limit that natural cellulose cannot be efficiently decomposed by purifying bacteria and clastic enzymes thereof, provides a key technology for straw in-situ rapid decomposition, and has a wide application prospect in the field of returning straws to the field and decomposing a lignocelluloses material.

The invention relates to a silage inoculant and a preparation method and application of the silage inoculant. The silage inoculant is prepared by respectively fermenting lactobacillus plantarum, bacillus subtilis and lactococcus and mixing the obtained fermentation broth, wherein the ratio of numbers of colony-forming units of lactobacillus plantarum to bacillus subtilis to lactococcus lactis in the mixed fermentation broths is (90-96) to (3.5-6) to (0.5-4), and the total number of colony-forming units is 0.8*10<12>-1.2*10<12>CFU / ml. Various lactic acid bacteria in the fungicide have a high synergistic effect, and form a stable eco-system, and non-sterile natural raw materials can be quickly propagated and colonized to hold advantages, so that large amounts of lactic acid are quickly generated, the pH value of fermented materials is reduced, the fermentation quality of a fodder is improved, the nutritional value and the palatability of the fodder are improved, the application is simple and easy, and the cost is low.

The invention belongs to the field of biotechnology, and relates to a method for expanding the hematopoietic stem / progenitor cells from umbilical cord blood in vitro by an alginate three-dimensional culture system. The method combines SCF, TPO and FL cytokines to support the expansion of the hematopoietic stem / progenitor cells in a single karyocyte of human umbilical cord blood, does not need adding other cytokines, and cultures the single karyocyte of human umbilical cord blood packaged in an alginate microsphere in a three-dimensional stationary culture system and a three-dimensional rotary culture system to observe the expansion effect of the single karyocyte of human umbilical cord blood packaged in the alginate microsphere in the rotary culture system. Compared with the conventional two-dimensional system, the three-dimensional culture system improves the total cells and obviously increases CD34+ cells under the condition of culturing low-concentration cytokines; and colony forming unit-granulocytes and macrophages of cells expanded in the three-dimensional culture system are cloned to form obviously increased cells, and the single karyocyte of human umbilical cord blood in a transplanted NOD / SCID mouse has better engraftment and reconstructs hemopoiesis.

High pressure processing (HPP) uses isostatic pressure to evenly apply great pressures to foods on all sides of the foods. Because the pressure is isostatic, the food is not deformed, so long as there is no internal cavity in the food. The pressures used are preferably from about 100 MPa (about 14.5 ksi) to about 1000 MPa (about 145 ksi), and the temperature rise is preferably limited to about 3° C. (about 5° F.) per 100 MPa (about 14.5 ksi). The high pressure dramatically reduces pathogens in the food so processed, as much as a four or five-log reduction in counts of colony forming units (CFU). The process has been shown to be effective in achieving these reductions in E. Coli, listeria, and salmonella. Pieces or sticks of beef jerky or sausage may be processed via HPP and combined with cheese to form a food product with a long shelf life.

The invention provides pleurotus eryngii yoghurt and a production method thereof, and belongs to the technical field of food science. The pleurotus eryngii yoghurt is milk white in color, has uniform and exquisite texture, moderate viscosity, faint fragrance of pleurotus eryngii and special aroma of fermented milk, and moderate soursweet, and contains not less than 3.0% of protein, not less than 3.1% of fat, not greater than 70 thorner degrees of acidity, and not less than 1*10<6>CFU (Colony Forming Unit) / mL of lactobacilli count. The production method comprises the following steps: getting pleurotus eryngii and milk as the major raw materials; slicing and pre-cooking the pleurotus eryngii; pulping the pleurotus eryngii with equivalent water; adding milk, granulated sugar, a stabilizer and a sweetening agent in proportion; homogenizing; sterilizing; cooling; adding 2.5% of lactobacillus bulgaricus and streptococcus thermophilus in proportion of 1: 1.5; filling; fermenting at 42 DEG C until the titration acidity reaches 65 to 75 thorner degrees; and stopping to refrigerate and after-ripen for 24 hours at 4 to 6 DEG C to obtain the pleurotus eryngii yoghurt. The production method has the beneficial effects that the whole pleurotus eryngii is pulped and added, thus the variety and content of nutritional components in the yoghurt are increased, the healthcare function of the yoghurt is improved, and new taste is created. The production method is important for promoting the rational processing and utilizing of pleurotus eryngii resources, formation of an after-production processing industry chain and adjustment of an agriculture product processing industry structure.

The invention provides an additive having a function of regulating intestines and stomach of a pet as well as preparation method and application thereof, and belongs to the technical field of animal feed. The additive having the function of regulating the intestines and the stomach of the pet is prepared from the following components in parts by weight: 2 to 10 parts of lactobacillus acidophilus powder, 1 to 5 parts of fructus crataegi, 2 to 10 parts of rhizoma dioscoreae, 2 to 10 parts of poria cocos, 1 to 5 parts of liquorice root and 1 to 5 parts of pericarpium citri reticulatae, wherein the viable bacteria concentration of the lactobacillus acidophilus powder is 0.5*10<10> to 1.5*10<10> CFU (Colony Forming Unit) / g. The additive provided by the invention is capable of obviously increasing anti-stress capability of puppies in a weaning stage and regulating the gastrointestinal function, an experiment shows that compared with a control group, the prevalence rate, the appetite, the skin and hair glossiness, the activity, the daily gain and the intestinal flora composition of an experimental group are all remarkably improved, and compared with a rhizoma dioscoreae and poria cocos taste-lack group, the prevalence rate, the appetite, the skin and hair glossiness, the activity and the daily gain of the experimental group have remarkable difference.

The invention discloses a probiotic fermentation beverage capable of preventing and controlling diabetes mellitus, and a preparation method thereof, and belongs to the technical field of fermentationbeverage preparation. In order to solve the problem that personalized eating is difficult in realization since an existing health care type probiotic fermentation beverages has a complex fermentationtechnology, the invention utilizes optimal lactic acid bacteria cultures suitable for fruit and vegetable fermentation and functional cultures capable of lowering blood glucose, and a fermentation accelerant is taken as an auxiliary material to prepare a lactic acid bacteria fermentation bacteria preparation. Under a family environment, the active lactic acid bacteria content of the probiotic fresh brewing fermentation beverage which has a low carbohydrate content, is proper in sour and sweet taste and has a special flavor is prepared through anaerobic fermentation for 60-96h at a constant fermentation temperature of 35-38DEG C through a standard operation specification for homemade fresh brewing fermentation beverages is as high as 2.9*10<10>CFU (colony-forming unit) / 100mL. The product disclosed by the invention is high in safety, does not have a side and effect function, and has a good application value and market prospect.