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Ripening fungicide for corn stalk substrate generation and application

A technology of corn stalks and antibacterial agents, which is applied in the field of resource utilization of agricultural waste, can solve the problems of limited substrate resources for seedling cultivation, rock wool is difficult to handle, and cannot be regenerated, and achieves accelerated decomposition, accelerated maturation progress, and degradation rate high effect

Inactive Publication Date: 2016-11-09
大庆市春田绿土农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, with the rapid development of industrial seedling raising technology and soilless cultivation technology, the demand for cultivation substrates is increasing rapidly. Common seedling cultivation substrates such as peat and vermiculite have limited resources and cannot be regenerated in a short time. Rock wool, etc. It is difficult to handle after use, and it is easy to cause pollution to the environment. It is urgent to develop new alternative cultivation substrates

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0035] Example 1-3 Preparation of mature bacterial agent for corn stalk matrix

[0036] The ripening microbial agent prepared in embodiment 1-3, its active ingredient is Clostridium thermocellum (Clostridiumthermocellum) preservation number DSM No.1237, cellulolytic xylanomonas (Xylanimonascellulosilytica) preservation number DSM No.15894, wax Bacillus cereus (Bacillus cereus) deposit number ACCC No. 03315 and Bacillus licheniformis (Bacillus licheniformis) deposit number ACCC No. 01958.

[0037] 1. Preparation of seed solution:

[0038] Inoculate Clostridium thermocellum preservation number DSM No.1237 in peptone cellulose (PCS) modified medium, culture it statically at 50°C for 2 days, and collect the fermented liquid as seed liquid;

[0039]Inoculate Xylanomonas cellulolyticum preservation number DSM No. 15894 in peptone cellulose-improved medium, culture it statically at 50°C for 2 days, and collect the fermented liquid as seed liquid;

[0040] Inoculate Bacillus cereus ...

Embodiment 4

[0046] Example 4 Application of maturing bacterial agent in decomposing fermentation of corn stalk matrix

[0047] Experimental group 1-experimental group 3: Mix corn stalks, urea and calcium carbonate at a mass ratio of 98:1:0.1, add water with a total weight of 60% of corn stalks, urea and calcium carbonate, and obtain mature fermentation raw materials with Embodiment 1-3 (embodiment 1, embodiment 2 and embodiment 3 correspond to experiment group 1, experiment group 2 and experiment group 3 respectively) the ripening bacterial agent prepared by 100:0.5 mass ratio, piles up into wide The 3m, 1.3m high, and 5m long cuboids are decomposed and fermented. The fermentation period is 20 days. When the temperature of the pile body exceeds 50°C during the fermentation process, the pile is turned once a day until the fermentation is completed.

[0048] Control group: without adding the mature bacterial agent prepared in Examples 1-3, the mature fermentation raw materials were directly...

Embodiment 5

[0053] Example 5 Application of Soilless Culture Substrate in Rice Seedling Cultivation

[0054] The fermentation product obtained in Example 4 was used as a soilless culture substrate for rice seedling cultivation.

[0055] Experimental group 1-3: Take the matrix of the experimental group obtained in Example 4, with a total dry weight of about 300g, and evenly spread it on a special thin plastic tray with a length, width, and height of 60×30×4cm. The thickness of the matrix is ​​about 3cm. After pressing, water the sulfuric acid water with a pH of 5.5, sow 100g (dry weight basis) germinated rice seeds per tray, cover the topsoil with a thickness of 0.5cm, and grow seedlings in a greenhouse for 30 days. The temperature simulates the spring conditions in Northeast China, 25°C during the day and 10-15°C at night. . Three repetitions were set up in the experiment, and the growth status of rice seedlings was measured after 30 days.

[0056] Control group: referring to the operat...

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Abstract

The invention provides a ripening fungicide for corn stalk substrate generation. Active ingredients of the ripening fungicide are clostridium thermocellum, xylanimonas cellulosilytica, bacillus cereus and bacillus licheniformis. The ratio of colony forming units of the four fungi is (4-5):(2-3):(2-3):(2-3), and the effective viable organism number of the fungicide is 109 / mL or above. The invention further provides application of the ripening fungicide to decomposing fermentation for corn stalk substrate generation. The ripening fungicide has a high corn stalk degradation rate, 40-70% of corn stalks can be decomposed within 20 days during large-scale ripening fermentation, an important technical means is provided for corn stalk substrate generation, and the ripening fungicide has broad application prospects in the field of organic culture substrate production.

Description

technical field [0001] The invention relates to the field of resource utilization of agricultural waste, in particular to a maturing bacterial agent for matrixing corn stalks and its application. Background technique [0002] In recent years, with the rapid development of industrial seedling raising technology and soilless cultivation technology, the demand for cultivation substrates is increasing rapidly. Common seedling cultivation substrates such as peat and vermiculite have limited resources and cannot be regenerated in a short time. Rock wool, etc. It is difficult to handle after use, and it is easy to cause pollution to the environment. It is urgent to develop new alternative cultivation substrates. my country is a large agricultural country, which produces a large amount of crop stalks every year, especially the huge output of corn stalks. If these corn stalks are matured and fermented at high temperature to achieve harmless treatment, a large amount of commercial cul...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C05G1/00C12R1/145C12R1/085C12R1/10C12R1/01
CPCC05C9/00C05F17/00C12N1/20C05D3/02C05F11/00C05F11/08Y02W30/40
Inventor 方春光
Owner 大庆市春田绿土农业科技有限公司
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