185 results about "Probiotic bacterium" patented technology

This invention provides a *Lactobacillus plantarum* LN-Lp1 strain, its applications, products, and methods, belonging to the field of probiotic technology. The *Lactobacillus plantarum* LN-Lp1 strain has the accession number CGMCC 33158. This LN-Lp1 strain was deposited on December 23, 2024, at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Based on this strain, this invention also provides its uses and related products. This LN-Lp1 strain exhibits strong resistance to simulated gastric-intestinal stress and good intestinal adhesion and colonization potential. The *Lactobacillus plantarum* LN-Lp1 of this invention has excellent weight-loss effects, improving lipid metabolism and lowering blood lipid levels.

The present invention discloses an anti-inflammatory Lactobacillus acidophilus composition for regulating immune responses, belonging to the fields of microbial technology and biomedicine. The composition comprises live lyophilized powders of Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium longum, together with homologous postbiotic lyophilized powders of the same strains. By combining functionally complementary probiotics with their homologous postbiotics, a full-chain anti-inflammatory system is constructed, covering immune regulation, inflammation suppression, and intestinal barrier repair. The live bacteria enable long-term colonization and microecological regulation, while the postbiotics act rapidly, producing a synergistic effect. The composition is prepared solely from strain-derived fermentation products without exogenous additives, ensuring safety and purity, and provides a stable and efficient solution for inflammation relief and immune function optimization.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

This application relates to the field of feed processing technology and discloses a highly absorbable feed composition for nursery pigs containing compound probiotics. The composition includes the following ingredients: a basal diet, a live bacteria compound powder composed of freeze-dried *Lactobacillus plantarum* powder and freeze-dried *Bacillus coagulans* powder, medium-chain triglycerides, a PIT-responsive compound emulsifier composed of polysorbate-80 and sorbitan monooleate, anhydrous trehalose, glycerol, a micron-sized confinement carrier, which is a porous carrier obtained by high-temperature expansion and vacuum degassing of perlite powder, and fumaric acid. This invention employs anhydrous trehalose and glycerol to construct a high-permeability system, combined with medium-chain triglycerides and a compound emulsifier to form an oil-phase encapsulation, achieving the technical effect of effectively blocking external moisture penetration and improving the heat resistance of the bacteria.

The application discloses a use of a pharmaceutical composition of a phenolic acid compound and probiotics, and particularly relates to a combination of chlorogenic acid and lactobacillus reuteri. The combination of chlorogenic acid and lactobacillus reuteri can improve glycolipid metabolic disorder, improve cognitive dysfunction, emotional disorder and delay aging, and can be used for preparing a medicine for treating metabolic disorder combined with neuropsychiatric diseases.

The application discloses a Qingjiang Aurantii Fructus extract with soothing and repairing effects on atopic dermatitis and application thereof, and a preparation method of the Qingjiang Aurantii Fructus extract comprises the following steps: A1, crushing Qingjiang Aurantii Fructus, adding water to soak, and then sterilizing; A2, inoculating probiotics in the solution after sterilization in step A1 to perform fermentation; A3, filtering the obtained fermentation liquor after sterilization, and purifying to obtain the Qingjiang Aurantii Fructus extract; wherein the probiotics are at least one selected from lactic acid bacteria, bacillus and yeast fungi. The Qingjiang Aurantii Fructus extract obtained by fermenting Qingjiang Aurantii Fructus with specific probiotics has significant soothing, repairing and anti-inflammatory effects, and has the soothing and repairing effects on atopic dermatitis in particular.

This invention discloses a probiotic liquid core ball cultured under polysaccharide confinement, its preparation method, and its application in improving children's intestinal flora. Belonging to the field of probiotic preparation technology, it employs an extrusion method combined with calcium alginate cross-linking technology, using xanthan gum and other thickening polysaccharides to optimize the types and concentrations of polysaccharides to construct the confinement space. The effects of different oxygen conditions on the encapsulation of different proportions of *Lactobacillus rhamnosus* LRa05 and *Bifidobacterium animalis* BLa80 were investigated to obtain the optimal probiotic liquid core ball cultured under polysaccharide confinement. This process is simple, uses safe raw materials, and after confinement culture, the viable bacteria count is ≥10⁹ CFU / g. It can directionally increase the abundance of beneficial bacteria in children's intestines, inhibit opportunistic pathogens, enhance flora diversity and stability, and is suitable for children's dietary scenarios, providing a safe and efficient intestinal flora regulation solution.

This invention discloses the application of *Lactobacillus plantarum* TWK10 in the preparation of health foods or drugs that enhance exercise motivation. *Lactobacillus plantarum* TWK10, with its Latin name *Lactobacillus plantarum*, was deposited on June 27, 2022, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC NO. 25190. This invention fills a technological gap in the field of probiotics for sports psychology intervention, providing a new strategy for "physiological-psychological" dual-effect intervention in sports nutrition, and can be further applied to weight management.

This invention provides a probiotic composition for improving diarrhea and its uses. The probiotic composition of this invention comprises space-occupying bacteria, organic acid-producing bacteria, and oxygen-depleting bacteria, wherein the oxygen-depleting bacteria are *Weizmannii*, the organic acid-producing bacteria are *Lactobacillus* or *Lactobacillus lactis*, and the space-occupying bacteria are *Bifidobacterium*. The probiotic composition of this invention can effectively improve diarrhea and is therefore suitable for the prevention and / or treatment of diarrhea-related functional gastrointestinal disorders.

This invention discloses a fermented microbial preparation for inhibiting Campylobacter jejuni in calves, its preparation method, and its application. The fermented microbial preparation is a co-fermentation product of a mixture of Lygodium japonicum decoction and liquid culture medium, inoculated with Lactobacillus paracasei; the Lactobacillus paracasei is... Lacticaseibacillus paracasei KQNIU. Comparative experiments in this invention revealed that, compared to other types of traditional Chinese medicine, the inhibitory effect of fermented Lygodium japonicum on Campylobacter jejuni is significantly higher than that of other types of traditional Chinese medicine. Furthermore, the co-fermentation of the traditional Chinese medicine Lygodium japonicum and the probiotic Lactobacillus paracasei KQNIU achieves a synergistic effect between the two, overcoming the problem of limited efficacy when used alone.

The application discloses Lactobacillus reuteri and application thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, and relates to the technical field of biological medicine. The application provides application of Lactobacillus reuteri and / or a metabolite thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, wherein the Lactobacillus reuteri is Lactobacillus reuteri (HG001). Limosilactobacillus reuteri The Lactobacillus reuteri is separated from feces of healthy people and used for preparation of the medicine for treating diffuse large B-cell lymphoma, and it is found that the Lactobacillus reuteri is confirmed as a probiotic strain for enhancing the chemotherapy effect of diffuse large B-cell lymphoma and can improve side effects caused by cyclophosphamide treatment of diffuse large B-cell lymphoma; and the Lactobacillus reuteri and the cyclophosphamide have a synergistic effect in treatment of diffuse large B-cell lymphoma.

The application provides a probiotic preparation and application thereof. The probiotic preparation comprises active ingredients and adjuvants, wherein the active ingredients comprise oxygen scavenging bacteria, organic acid producing bacteria and space-occupying bacteria; the oxygen scavenging bacteria are Weizmannia bacteria, the organic acid producing bacteria are lactobacillus or lactobacillus plantarum, and the space-occupying bacteria are bifidobacterium; the water activity of the probiotic preparation is less than or equal to 0.3, preferably 0.04-0.3, further preferably 0.04-0.2, and more preferably 0.04-0.15. The probiotic preparation has high live bacteria stability when stored at room temperature. Moreover, the probiotic preparation can effectively improve functional gastrointestinal diseases and has bidirectional regulation function, and is suitable for different intestinal motility disorders, such as diarrhea and constipation.

This invention belongs to the field of vinegar brewing technology, and provides a multi-strain co-fermentation selenium-enriched active probiotic functional vinegar and its preparation method. Jujube enzymatic hydrolysate, malt juice, and ultrafine powder of Astragalus-Codonopsis compound traditional Chinese medicine are mixed in a certain proportion, and sodium selenite is added. Alcoholic fermentation is carried out using Saccharomyces cerevisiae CGMCC 34906. After alcoholic fermentation, acetic acid and lactic acid fermentation is carried out using a compound inoculum of Lactobacillus plantarum and Acetobacter pasteurization. After fermentation, the mixture is centrifuged, filtered, sterilized, post-ripened, blended, and sterilized. Active selenium-enriched Lactobacillus plantarum microcapsules are added, resulting in a double enhancement of selenium enrichment and active probiotics, yielding a selenium-enriched active probiotic functional vinegar. The vinegar is reddish-brown in color and has a complex aroma of jujube and malt fermentation, along with the aroma of traditional Chinese medicine. It has a mellow and pure sour taste with a long aftertaste. The flavor is rich and pleasant; total selenium ≥ 0.10 mg / L, and the number of viable lactic acid bacteria ≥ 10. 7 CFU / mL, polysaccharides ≥60.00 mg / 100 mL, saponins ≥2.50 mg / 100 mL.

This invention discloses a strain of *Pediococcus pentosaceus*, FREE-U AP01, and its applications. The strain, FREE-U AP01, has the accession number CGMCC No. 37267. This invention screened and obtained a strain of *Pediococcus pentosaceus*, FREE-U AP01, which, through its excellent temperature adaptability, high survival rate in acid and bile salt tolerance, rapid acid production capacity, and significant antibacterial activity, solves the problems of low survival rate and unstable antibacterial effect of existing probiotics, providing a powerful and high-quality strain resource for microecological preparations and animal feed additives.

This invention relates to *Lactobacillus paracasei* NEFU-5 and its applications, belonging to the field of biotechnology. To address the problems of limited functionality and poor direct degradation of metabolites in existing probiotic strains, this invention provides *Lactobacillus paracasei* NEFU-5 (accession number CCTCC NO: M 2026147). This *Lactobacillus paracasei* NEFU-5 can simultaneously and efficiently degrade uric acid, triglycerides, and cholesterol, and possesses excellent probiotic properties and safety. The *Lactobacillus paracasei* NEFU-5 provided by this invention can be used to prepare fermented products and can be used to prevent or improve hyperuricemia, hyperlipidemia, and other concurrent metabolic diseases, showing significant application prospects.

The application discloses a novel lactoferrin inducible soluble expression system and a construction method thereof, and belongs to the technical field of biotechnology and genetic engineering, and the specific scheme is that human lactoferrin (LF) is expressed by probiotic Escherichia coli Nissle1917, the protein is purified after the expression position is determined, and anti-inflammatory activity is determined. The LF expression system constructed by the application provides a construction method of a prokaryotic expression stable human lactoferrin expression vector, the vector is introduced into probiotic Escherichia coli Nissle1917, soluble target recombinant human lactoferrin is obtained after induction fermentation expression, two-step purification and identification, and the application has the characteristics of high safety, low cost, easy operation and the like.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The application provides a lactic acid bacteria with probiotic properties and application thereof, and the lactic acid bacteria is Lactobacillus plantarum (cc_Lp14) preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No. 37211 and a preservation date of December 26, 2025. Lactiplantibacillus plantarum The lactic acid bacteria screened by the application can tolerate high-sugar and high-acid environment of fruit and vegetable juice, has strong oxidation resistance, and can significantly improve the content of total phenols and flavonoids when applied to fruit and vegetable juice fermentation, thus having significant application potential in the field of fruit and vegetable juice fermentation and providing important strain resources for development of a new generation of probiotic fermentation agents.

This invention provides a *Lactobacillus reuteri* AUI2506 strain and its applications. The *Lactobacillus reuteri* AUI2506 strain of this invention is a probiotic that grows rapidly, requires simple fermentation conditions, is biosafe, and exhibits good acid and bile salt tolerance, auto-aggregation ability, and surface hydrophobicity. It survives for a long time and maintains high activity in the gastrointestinal tract, contributing to positive health effects. Furthermore, this strain possesses antioxidant, anti-inflammatory, weight-loss, blood sugar-lowering, and adjunctive blood sugar-lowering effects, filling a gap in existing technologies and providing a new and effective means to improve metabolic health.

The application discloses a preparation method and application of a composite strain of a sugar-controlling probiotic, and belongs to the technical field of microorganisms. The method comprises the steps of strain stock solution preparation, anaerobic culture, pH value determination and regulation, and composite strain proportioning. Through a dynamic shunting mechanism with pH value 4.3 as a threshold, the culture solution lower than the value is subjected to re-acidification treatment, and the culture solution higher than the value is subjected to differential dilution and coating operation. Finally, Lactobacillus gasseri, Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus paracasei, Bifidobacterium breve, Bifidobacterium lactis and Bifidobacterium longum are mixed at a preset ratio, and a sustained-release agent is added for storage. The method can significantly improve the screening efficiency of target probiotics, the stability of the composite preparation and the blood glucose regulation effect, and can be widely applied to the development of sugar-controlling probiotic products.

The application discloses a probiotic composition and a production process of a probiotic fermented bread crumb, and belongs to the technical field of bread crumb processing. The probiotic composition is a microcapsule structure containing malt dextrin, arabic gum and heat-denatured whey protein isolate as wall materials, and lactobacillus acidophilus and lactobacillus rhamnosus as core materials. The structure can ensure that a small amount of live bacteria and metabolic products are slowly released through the micropores of the wall materials in a normal temperature fermentation environment, so that the free lactic acid bacteria are prevented from rapidly proliferating, excessively producing acid and competing with yeast for carbon and nitrogen sources in the initial stage of fermentation. The probiotic composition can provide nutrition for the yeast in the fermentation process, promote cell division and metabolic activity of the yeast, and the polydextrose, inulin and arabic gum in the microcapsule wall materials are hydrophilic colloids, which can form a uniform hydration network in the dough.

This invention discloses the application of *Lactobacillus animalis* ATCC35046 in the preparation of anti-aging drugs. The *Lactobacillus animalis* ATCC35046 involved in this invention is colonized in dental plaque of primates and in the intestines of dogs and mice. It can significantly reduce the expression of P16 or P21 in brain, bone, muscle, and skin tissues, significantly downregulate the level of aging-related secretory phenotypes in serum, and improve spatial memory, motor function, and muscle function. This provides a theoretical reference and guidance for the development of multi-target, multifunctional anti-aging probiotic preparations.

This invention discloses a kidney-tonifying and liver-nourishing compound probiotic Cistanche fermented drink and its preparation method, comprising the following steps: mixing and sterilizing Cistanche deserticola, Poria cocos, malt, functional components, and natural selenium-strontium mineral water to obtain a fermentation substrate; activating *Lactobacillus casei*, *Lactobacillus acidophilus*, *Lactobacillus rhamnosus*, and *Lactobacillus plantarum* strains respectively, centrifuging and freeze-drying, and then mixing them to obtain a compound probiotic powder; culturing the compound probiotic powder in MRS medium to obtain a compound probiotic liquid; inoculating the compound probiotic liquid into the fermentation substrate for fermentation, sterilizing, and obtaining the kidney-tonifying and liver-nourishing compound probiotic Cistanche deserticola fermented drink. This invention, through the innovative integrated formulation technology of combining Cistanche deserticola, malt, and Poria cocos as medicinal and edible ingredients, multifunctional factor compounding, and compound probiotic fermentation, prepares a kidney-tonifying probiotic Cistanche deserticola fermented drink with high active ingredients, clear efficacy, and excellent flavor.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

This invention discloses a *Lactobacillus plantarum* bacterial powder, its preparation method, and its application. The wall material of the bacterial powder includes resistant starch, whey protein, and a heat protectant. The bacterial cells in the powder are *Lactobacillus plantarum* TH103. The mass percentages of resistant starch, whey protein, and the heat protectant are 7-23%, with the total mass percentage of resistant starch and whey protein being 3-15% and the mass percentage of the heat protectant being 4-8%. The viable count of the bacterial powder is (3.5-9.0) × 10⁻⁶. 9 The CFU / g and moisture content are 5-7%. This invention uses resistant starch and whey protein as protective carriers, supplemented with heat-resistant protective agents, to achieve the preparation of *Lactobacillus plantarum* powder by spray drying. The obtained *Lactobacillus plantarum* powder has a viable count and moisture content that meet the relevant requirements for probiotic foods, and has good powder properties. It can be directly brewed in powder form or applied to fermented foods such as yogurt.

The present invention relates to a Lactobacillus rhamnosus strain. In particular, the present invention relates to a composition comprising a Lactobacillus rhamnosus optionally in combination with a further lactobacilli and the use of the same in the treatment, prevention or alleviation of candidiasis.

A probiotic composition for treating or preventing metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma(MASLD-HCC) or colorectal cancer, comprises one or more microbiota selected from a group consisting of Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus GG, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis, Streptococcus thermophilus or any combination thereof. The probiotic composition demonstrates improved therapeutic effects when combined with low-dose Sorafenib for MASLD-HCC and outperforms 5-Fluorouracil in colorectal cancer models.