1523 results about "Probiotic bacterium" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

This invention relates to a composition suitable for use in the prevention of opportunistic infections in immune-compromised individuals comprising a probiotic Bifidobacterium lactis, Bifidobacterium infantis, Bifidobacterium breve or Bifidobacterium longum and a fucosylated oligosaccharide selected from the group comprising 2′-fucosyllactose, 3′fucosyllactose, difucosyllactose, lacto-N-fucopentaose, lacto-N-fucohexaose, fucosyllacto-N-hexaose and fucosyllacto-N-neohexaose. The invention further extends to the use of such a composition in the prevention of opportunistic infections in immune-compromised individuals.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.

The invention relates to a technology for high-density fermentation of Lactobacillus casei (Lactobacillus casei Zhang) with prebiotic efficacy, which comprises optimal controls on a proliferation culture medium and the condition of a fermentation process, and a technology for preparing high-vitality freeze-dried powder of the strain. With the technology, the freeze-dried powder of the Lactobacillus casei with more than 10<11> cfu per milliliter of viable bacteria can be obtained, and the freeze-dried powder can be directly applied to the preparation and the application of a probiotic hametsz and a probiotic preparation of the strain.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The invention provides a lactobacillus casei strain and a probiotic composition for lowering blood uric acid and application thereof, and belongs to the technical field of food medicines. The lactobacillus casei strain ZM15 for lowering the blood uric acid has the collection number of CGMCC No.13980. The lactobacillus casei strain ZM15 has the rates of degrading adenylic acid, guanylic acid, adenosine and guanosine as high as 100%; a degraded product does not contain uric acid and allantoin; in addition, the degraded product has a low purine base proportion of only 0.10%, so that the product can be prevented from being further decomposed into the uric acid, and the human blood uric acid content can be effectively reduced. The probiotic composition provided by the invention is obtained by compounding four strains of ZM15, ZM18, ZM122 and ZM05; through mutual coordination of the four strains, the blood uric acid lowering effect of the probiotic composition is significantly better than that during independent application of ZM15, ZM18, ZM122 and ZM05 strains, so that the blood uric acid lowering effect can be further improved.

The invention discloses a lactobacillus plantarum and a preparation method thereof for high-density culture and freeze-drying bacteria powder, and has the technical scheme that the lactobacillus plantarum (Lactobacillus plantarum P8) is probiotics separated from the traditional fermentation milk product (yoghurt) of the Urat Middle Banner of Inner Mongolia Bayan Nur. The probiotics has the characteristics of artificial gastric juice and artificial digestive juice tolerance, cholate tolerance, intestinal agglutination action and common pathogenic entero bacteria inhibition. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5468 on 18th, Nov., 2011.

A method for producing probiotic feed for animals by fermenting high-fiber agricultural and sideline products with complex bacteria. , and / or 0-1.0% calcium chloride and / or 0-1.0% magnesium sulfate) are mixed in proportion and then steamed with water; after the steaming is completed, cool to 20-40°C and inoculate Neurostria fortis and Lactobacillus plantarum is solid-state fermented at 20-40°C for 2-5 days; the fermented feed is dried at 40°C-70°C and crushed to obtain a probiotic feed product rich in carotenoids. The method has the advantages of high production efficiency, low cost and good benefit, and while solving the problem of environmental pollution, it also relieves the pressure of serious shortage of energy and protein feed sources.

Chimeric proteins are expressed, secreted or released by a bacterium to immunize against or treat a parasite, infectious disease or malignancy. The delivery vector may also be attenuated, non-pathogenic, low pathogenic, or a probiotic bacterium. The chimeric proteins include chimeras of, e.g., phage coat and / or colicin proteins, bacterial toxins and / or enzymes, autotransporter peptides, lytic peptides, multimerization domains, and / or membrane transducing (ferry) peptides. The active portion of the immunogenic chimeric proteins can include antigens against a wide range of parasites and infectious agents, cancers, Alzheimer's and Huntington's diseases, and have enhanced activity when secreted or released by the bacteria, and / or have direct anti-parasite or infectious agent activity. The activity of the secreted proteins is further increased by co-expression of a protease inhibitor that prevents degradation of the effector peptides. Addition of an antibody binding or antibody-degrading protein further prevents the premature elimination of the vector and enhances the immune response.

The invention relates to a stirring type cereal sour milk and a method of preparing the same. The cereal sour milk is made through lactobacillus and probiotics ferment by using raw materials with the following weight components: raw fresh milk of 700-875 parts, cereal powder 1-30 parts; additive agent for dairy food of 5-44 parts; lactobacillus strain / functionality probiotics strain of 0.2-0.8 parts; corrective of 48-82 parts; stabilizing thickening agent of 3-10 parts; homogeneous pourable dense quasi-fluid which is the external appearance showing the color of added cereal powder with suspended corresponding cereal powder; the tissue is fine, smooth and uniform and a few whey is allowed to be precipitated; organoleptic attribute, nutritive index, health indicator and content of lactobacillus series of the cereal sour milk are all better than that in the GB2746 cultured milk quality index. The present invention is characterized in that the cereal powder that is formed by pulverizing and swelling processes reserves the nutrient of cereal to the utmost extent and improves the hot water leaching rate and absorption rate of its nutrient components; the technique is more compact and the operation is easy; the production efficiency is high and the consumption human populations of the products are widened; the cereal sour milk is easy to digested and absorbed, and the taste is better.

The invention discloses fermented fruit and vegetable composition containing probiotics and traditional Chinese medicine extract and a preparation method of the fermented fruit and vegetable composition. The fermented fruit and vegetable composition comprises fermented fruit and vegetable, fermented or non-fermented traditional Chinese medicine or the traditional Chinese medicine extract, the prebiotics, probiotics and an auxiliary, wherein the prebiotics are formed by one or more oligosaccharides and the probiotics comprise one or more of lactobacillus, bifidobacterium and streptococcus; the fruit and vegetable comprise one or combination of pumpkins, white gourds, kiwi fruits, soybeans, papayas, purple sweet potatoes, carrots, Chinese chestnuts, Chinese yam, maca fruit, pearl barley, Wolfiporia extensa, Angelica sinensis, kudzu vine roots and lotus seeds; the traditional Chinese medicine or the traditional Chinese medicine extract is one or more of Astragalus, olive leaves, grape seeds, ginger, gardenias, saw palmetto, pumpkin seeds, pomegranate seeds, pomegranate bark, raspberries, Cistanche deserticola, black cohosh, red clover or extract thereof. The fermented fruit and vegetable composition is food, a medicine or a health care product with the efficacy of regulating intestinal flora, boosting immunity, improving sleeping function and the like.

The invention provides a composition. The composition is prepared from the following components in parts by weight: 20 to 80 parts of collagen peptide, 2 to 40 parts of acerola cherry powder, and 0.1to 20 parts of lacid bacteria compound powder. The composition has the advantages that by comprising the collagen peptide, the acerola cherry powder and prebiotics according to a special ratio, the components can reach the synergistic function, the skin condition is fundamentally improved, and the beautiful face is restored; the regeneration of skin cells is accelerated, the water and oil balanceof the skin is regulated, and the problems of skin pigmentation, coarse pores, fine lines, red blood silk, facial infection, and uneven skin color are relieved; the face-beautifying and oxidizing-resistant characteristics of the collagen peptide and acerola cherry powder are fully realized, the collagen peptide and acerola cherry powder are cooperated with the prebiotics strain beneficial for theprivate part health of special women to reach the effect of regulating micro-ecological balance and hormone balance of women, and the private part health is improved.

The present disclosure generally relates to pediatric nutritional compositions including a prebiotic mixture of galacto-oligosaccharide and / or polydextrose, a probiotic, such as Lactobacillus rhamnosus GG, and human milk oligosaccharides. More particularly, the present disclosure relates to a nutritional composition having: (i) a protein source, (ii) a lipid source, (iii) a carbohydrate source, (iv) a human milk oligosaccharide or a precursor thereof, (v) polydextrose and / or galacto-oligosaccharides, and (vi) a probiotic. The disclosed nutritional compositions advantageously promote the gut-brain axis.

The present invention provides one kind of feed level microbe additive and its preparation and application. The microbe additive includes mainly probiotics part and nutritious liquid part. The probiotics part contains acidophilic lactobacillus, fecal enterococcin and butynic clostridium; and the nutritious liquid part contains acetic acid, propanoic acid and butyric acid. The feed level microbe additive is taken by chickling in 24 hr after hatching, and this can reduce the use amount of antiseptic medicine obviously, raise daily weight gain, lower the feed / meat ratio and raise economic utility obviously. In addition, it has simple preparation and low cost.

The invention discloses a fermented ginger and a manufacturing method thereof; the manufacturing method comprises the following steps that ginger is adopted as raw material, washed, cut into pieces or block, dried and sterilized; probiotic fermenting liquor is accessed into the ginger raw material, heat-preserved and fermented at the temperature of 28 DEG C to 39 DEG C; the used probiotics is lactobacillus, mould or bacillus spp, which can produce cellulase, amylase and protease, etc. and degrade cellulose, starch, protein and other macromolecular substances in the ginger; the invention adopts the probiotics as a zymogenic agent, and due to the existence of the probiotics, the growth of competitor is restrained in the processing, the storage period and the safety of the ginger is improved, the flavor of the ginger is improved, the nutrition of the ginger is strengthened, the bioactivity, the health and the utilization rate of the ginger are improved; the fermented ginger can be used as general seasoning, can also be used as the development of serial health foods with simple manufacturing process and stable result and is suitable for industrial production.

The invention relates to the preparation of a Lactobacillus casei Zhang strain and an application of the strain at the aspect of antioxidant products. The Lactobacillus casei Zhang strain is an acid- and bile acid-resistant probiotics strain separated from Lactobacillus casei Zhang of fermented mare milk which is the traditional dairy product of the inner Mongolia nationality; and the strain has been stored in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the Collection No. is: CGMCC No.1697. Besides the beneficial function to body health possessed by common lactobacillus, the Lactobacillus casei Zhang of the invention can also be used for the production of anti-oxidation functional products, such as: the production of various dairy products with antioxidant activity; and the Lactobacillus casei Zhang can be present in specific products by mixing with other materials, and can also be produced in the form of live bacteria preparation.

The invention discloses a method for preparing a probiotic brown lactobacillus beverage product with large amount of bacteria, which comprises the steps of Maillard reaction, probiotic fermentation and post treatment, wherein the probiotic fermentation step comprises: hydrating and sterilizing defatted milk powder or defatted fresh milk, reacting the defatted milk powder or the defatted fresh milk for 2.5 hours at the temperature of between 95 and 98 DEG C till the milky yellow becomes brown at the end point of the reaction, cooling the reaction product to between 37 and 38 DEG C, inoculating the reaction product to a Lactobacillus casei strain, statically fermenting the strain for 48 to 72 hours at the temperature of between 37 and 38 DEG C till the pH reaches 3.8 to 4.0, and cooling the fermentation product to between 2 and 6 DEG C; and the post treatment steps comprises: preparing base solution by using sugar, high fructose corn syrup, pectin and purified water, wherein the weight ratio of the base solution to the probiotic fermentation solution is 1 to 3; and the pH is adjusted to between 3.5 and 3.6; homogenizing the base solution, cooling the base solution to between 2 and 6 DEG C, canning products after passing detection, maturing the products for 4 to 8 hours at the temperature of between 2 and 6 DEG C, and delivery the products from a storage. The number of the living bacteria in the shelf life of the product can reach 1*108 to 1*1,010cfu / ml; and the product has zero fat and super clear mouthfeel, has no viscosity, has high probiotic detection rate, and can effectively keep or recover the intestinal microbial balance.

The invention relates to a method for preparing selenium-rich low-cholesterol egg and the relevant product, belonging to health-care functional egg product production field, which comprises following steps: employing lactobacillus acidophilus and candida as bacteria, the culture medium is 0.5-2% yeast concrete and 0.5-2% glucose, inoculating bacteria in sodium selenite containing culture medium, culturing for 30-40hours, getting culturing solution containing selenium-rich probiotics, adding culturing solution in basic corn bean meal type ration , feeding chicken with it, and getting selenium-rich low-cholesterol egg products. The selenium content in egg products is 0.358-0.508 mg / kg (wet weight), cholesterol in egg yellow content is 11.37-12.17 mg / g (wet weight). The egg product is characterized by abundant nutrition, selenium supplement, low-cholesterol content, health-care function and effect of prevention of cardiovascular disease.

The invention relates to fermented chenopodium quinoa willd milk, flavored fermented chenopodium quinoa willd milk, a chenopodium quinoa willd sour milk beverage, and a preparation method of products, and belongs to the technical field of food industry. Chenopodium quinoa willd is used as a raw material for pulping, the protein content is not less than 2.9g / 100g, a yoghourt fermenting agent is inoculated, fermentation is performed until acidity reaches to 70DEG T or above so that the fermented chenopodium quinoa willd milk is obtained. The yoghourt fermenting agent comprises a mixed bacterium agent which is obtained by mixing lactobacillus bulgaricus with streptococcus thermophilus in the mass ratio of the lactobacillus bulgaricus to the streptococcus thermophilus being 1 to 1 and of which the inoculum concentration is 20-25g / T, a mixed bacterium agent in which the mass ratio of lactobacillus acidophilus to bifidobacteria and to lactobacillus casei is 1 to 1 to 1 and of which the inoculum concentration is 12-15g / T, and lactobacillus plantarum of which the preservation number is CGMCC No.11763 and of which the inoculum concentration is 60-80ml / T. The chenopodium quinoa willd is used as the raw material, composite enzyme hydrolysis and composite active probiotic fermentation are used, and active probiotic fermented chenopodium quinoa willd milk and series products are prepared, so that industry blank is filled.

A pet food including a mixture of at least one probiotic micro-organism and oil, wherein the pet food is vacuum coated with said mixture, a production plant for making the same, and methods of making and using the same are provided.

Variant of the bacterium Lactobacillus fermentum and its use in treating gastrointestinal disorders, disorders of the mucosal surfaces or disturbances in the immune system or status of a subject.

The invention discloses a method for producing a microorganism feed additive by utilizing probiotics and mixed meal. The method comprises the following steps: adding water into fermentation substrates respectively, stirring materials, and then cooking and sterilizing; adding a mixed bacterial liquid into the sterilized fermentation substrates and stirring uniformly after the temperature is lowered to 28-35 DEG C, and carrying out aerobic fermentation under constant temperature; introducing a lactic acid bacterium after the fermentation substrates are fermented and mixed, and then carrying out secondary fermentation; and drying materials after fermentation at a lower temperature, and crushing so as to obtain a microorganism feed additive finished product. The fermentation substrates comprise germ meal, corn spray slurry fibers, rapeseed meal and rice bran meal; the rice mixed bacterial liquid is obtained through mixing bacterial liquids of candida utilis, bacillus licheniformis and cryytococcus neoformans; and the lactic acid bacterium is lactobacillus plantarum, and the inoculum size is 5-10% of the weight of the fermentation substrates.

The invention relates to a microcapsule embedding preparation method of a Lactobacillus casei Zhang microcapsule Zhang CGMCC No.1697, comprising the following steps: activating Lactobacillus casei strain, preparing culture medium, preparing strain fermentation liquor, preparing microcapsule wall material, preparing microcapsule embedding solution and microcapsule.Compared with Lactobacillus casei which is not embedded, the Lactobacillus casei microcapsule prepared by the method of the invention, after being processed in artificial digestive juice, can have viable count increased by one magnitude order.After being preserved for 30 days at 37 DEG C, the Lactobacillus casei microcapsule can have viable count increased by more than one magnitude order.Therefore, the Lactobacillus casei microcapsule prepared by the method of the invention can greatly improve efficacy of probiotics in the process of processing, storage and transportation.

The invention discloses a method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation and belongs to the technical field of microbial feed. The method comprises the following concrete steps that: firstly, protein feed is subjected to impurity removal and crushing, nutrition substances are added, mixed and stirred with clean water, and the moisture content is regulated to 60 to 70 percent; then, yeast, bacillus licheniformis and bacillus subtilis strain liquid is inoculated, high-moisture-content aerobic fermentation is carried out for 12 to 96 hours, and the fermentation temperature is 35 to 45 DEG C; then, energy feed is added, mixing and stirring are carried out, and the moisture content is regulated to 25 to 35 percent; next, lactobacillus lactis, enterococcus faecium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus plantarum strain liquid is added, complex enzyme is added, the low-moisture-content anaerobic fermentation is carried out for 24 to 120 hours, and the fermentation temperature is 25 to 35 DEG C; and finally, the complete feed finished product is prepared through rolling tube drying, mixing and stirring, granulation and package. The method has the beneficial effect that the production efficiency and the feed nutrition value are improved.

The invention relates to a kind of Se-enhanced amino acid yoghourt manufacture method and the product hemogenization the mixture of the fresh milk (ewes' milk or remade milk) and the sugar, then high temperature sterilization, cooling down, adding the the Se amino acid transform and the probiotics growth accelerant, then inoculating the Bifidobacterium, Strptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus bulgaricus, fermentation and cool storage. The cfu of the Bifidobacterium, Strptococcus thermophilus, Lactobacillus acidophilus , Lactobacillus bulgaricus will be up to 109 / mL,107 / mL,108 / mL,108 / mLand 108 / mL the Se concentration will be up to 0.1-0.32mg / L, among this, 50% is from the Se-amino acid. Such product is safety and integrated multi-function contained with the yoghourt, probiotics and the organic Se. It will do benefit to our body entironment, anti-pathogeny microorganism. It can enhance the body immunity function and illness resistance.