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This invention discloses a composite conductive organic hydrogel, prepared by the following method: First, acrylamide, carboxylated cellulose nanofibers, and carboxymethyl chitosan are copolymerized in an aqueous glycerol solution to obtain a pregel. Then, pyrrole is incorporated into the pregel, and subsequently, pyrrole is oxidized using a ferric chloride solution, causing it to polymerize in situ into polypyrrole. The hydrogel of this invention uses polyacrylamide as the network framework, incorporating carboxylated cellulose nanofibers and carboxymethyl chitosan, and polymerizing polypyrrole in situ to form a multi-layered cross-linked network. The hydrogel of this invention possesses excellent mechanical properties, strong adhesion and easy peeling, high electrical conductivity, excellent freeze resistance, and biocompatibility. It can be used as a high-performance flexible strain sensor for human motion monitoring or as a bioelectrode for electrocardiogram signal acquisition, showing broad application prospects in wearable electronics and health monitoring.