49 results about "Tissue engineering scaffold" patented technology

A method for preparing a magnesium alloy tissue engineering scaffold with a smooth inner surface by laser powder bed fusion (LPBF) is provided. The method includes: step S1: scanning a porous scaffold, and selecting the densest filling scan parameters, where the scanning includes a single-pass contour scan and a single-pass filling scan; step S2: optimizing a contour scan strategy according to the densest filling scan parameters; step S3: acquiring a corresponding melt pool dimension, and adjusting a spot compensation value based on the optimized contour scan strategy; and step S4: preparing a magnesium alloy tissue engineering scaffold based on the contour scan strategy and the adjusted spot compensation value. The method eliminates powder adhesion and sagging defects inside the complex porous structure that severely affect inner surface roughness and scaffold performance, thereby the pore connection, fatigue and corrosion resistance will be improved greatly.

The application provides a biocompatible biological aerogel material and a preparation method thereof, and the method comprises the following steps: cleaning and roughening pretreatment of a substrate; dissolving polyvinyl alcohol to prepare a precursor solution; introducing wear-resistant reinforcing materials into the precursor solution and uniformly dispersing the wear-resistant reinforcing materials; adding a crosslinking agent, glutaraldehyde, to perform a crosslinking reaction and construct a three-dimensional network structure; coating the system in the initial stage of crosslinking on the substrate, and performing low-temperature or freeze-drying and subsequent heat treatment to form a stable coating. The obtained material takes polyvinyl alcohol as a biocompatible skeleton, and has excellent porous structure, mechanical properties and wet stability through chemical crosslinking and reinforcing phase compounding. In-vitro cell experiments show that the material has no obvious toxicity to cells, and the cells adhere and spread well. The application solves the problem that the existing biological aerogel material is difficult to consider the biocompatibility, structural stability and process friendliness, and is suitable for the biomedical field such as tissue engineering scaffolds and wound dressings.

The invention provides PVDF / gelatin / CNF composite hydrogel capable of enhancing piezoelectric property based on an in-situ stretching effect and a preparation method and application thereof. According to the composite hydrogel, multi-directional constraining force is applied to a PVDF molecular chain through reaction among CN, multivalent metal ions in a cross-linking agent and PVDF; and the in-situ stretching polarization is realized by promoting the transformation from a non-piezoelectric alpha / gamma phase to a piezoelectric beta phase with an all-trans conformation. The composite hydrogel has good biocompatibility, degradability, high strength, high toughness and fatigue resistance, is mild in preparation condition and low in energy consumption, and has huge application potential in the fields of wearable stress / strain sensors, self-powered implantable medical equipment, high-performance energy collectors, bearing tissue engineering scaffolds and the like.

The invention provides an enzyme-residue-free extracellular matrix microsphere as well as a preparation method and application thereof. An elutable sacrificial phase material capable of being subjected to liquid-liquid phase separation with an extracellular matrix is introduced in an extracellular matrix pelletizing process, and transglutaminase is confined in a sacrificial phase, so that the extracellular matrix microsphere is prepared. And the transglutaminase and the extracellular matrix form a spatially separated structure in the balling process. In the gel forming stage, transglutaminase catalyzes protein in an extracellular matrix on a two-phase interface to generate a cross-linking reaction, and microspheres with a three-dimensional network gel structure are formed; after cross-linking is completed, transglutaminase is removed by eluting the sacrificial phase material, and meanwhile, a porous network with a communicated structure is formed in situ in the microsphere, so that the obtained extracellular matrix microsphere still keeps good structural integrity and mechanical property while realizing no residue of enzyme with catalytic activity; the scaffold can provide stable support for cell adhesion, migration and three-dimensional culture, and is applicable to cell delivery, organoid construction, tissue engineering scaffolds and the like.

The invention provides a porous tissue engineering scaffold with a drug controlled release function as well as preparation and application of the porous tissue engineering scaffold. The preparation method of the porous tissue engineering scaffold comprises the following steps: S1) preparing double-layer nanoparticles by PLGA-PEG; and S2) preparing a composite scaffold by a low-temperature 3D printing technology. The stent prepared by the method has efficient drug controlled release capability, excellent mechanical properties, good biocompatibility and controllable degradation. The optimized porous structure promotes cell proliferation and tissue regeneration, meanwhile, low-temperature printing keeps the stability and activity of materials and drugs, and the material is widely applied to the fields of bone tissue engineering, local drug delivery and the like and shows remarkable clinical application value and market potential.

This invention provides a biomimetic periodontal complex multilayer hydrogel tissue engineering scaffold, its preparation method, and its application, relating to the technical field of tissue engineering scaffolds. The biomimetic periodontal complex multilayer hydrogel tissue engineering scaffold comprises, from the inside out, a cementum repair layer, a periodontal ligament repair layer, and an alveolar bone repair layer; the cementum repair layer and the alveolar bone repair layer include a mineralized hydrogel layer; wherein the raw materials for preparing the mineralized hydrogel layer include modified hyaluronic acid, methacrylamide type I collagen, and hydroxyapatite; the alveolar bone repair layer includes a non-mineralized hydrogel layer; wherein the raw materials for preparing the non-mineralized hydrogel layer include modified hyaluronic acid, methacrylamide type I collagen, and fibrin I. This invention precisely matches the mechanical and biological requirements of each layer of the periodontal complex through a three-layer structure, a reversibly hydrazone-regulated viscoelastic gradient, and a differentiated biochemical microenvironment of nHAp / FBN1.

The invention discloses a protein controllable self-assembly-based mild universal hydrogel toughening method, which is characterized in that more rigid and hydrophobic beta-folded ordered stacking structures are formed by controlling the transformation of protein macromolecular structures, and the mechanical properties of protein-based hydrogel are enhanced by cooperating with ionic physical cross-linking immobilization; through the synergistic effect of a physical network and a chemical network, the toughness, the strength, the elasticity and the anti-fatigue property of the hydrogel are remarkably improved under a completely mild condition. The method does not need toxic cross-linking agents or violent reaction conditions, has wide selectivity, is suitable for various protein raw materials, and opens up a new way for preparing the protein-based tough hydrogel, and the prepared hydrogel has wide application prospects in the fields of flexible sensors, tissue engineering scaffolds, drug sustained-release carriers, wound dressings or environmental engineering and the like.

The invention belongs to the technical field of tissue engineering scaffolds, and relates to a micro-nano fiber patterned tissue engineering scaffold and a solution electrostatic spinning preparation method thereof, and the micro-nano fiber patterned tissue engineering scaffold is formed by randomly interlacing and compounding uniformly distributed micron-scale skeleton fibers and nano-scale filling fibers on a patterned receiving electrode, the diameter of the micron-scale framework fiber is 15-25 microns, and the diameter of the nano-scale filling fiber is 200-500 nm; the nano-scale filling fibers are distributed on the surfaces of the micron-scale skeleton fibers and among different micron-scale skeleton fibers, and the micron-scale skeleton fibers and the nano-scale filling fibers penetrate through each other to form a three-dimensional graded fiber network structure; the patterned receiving electrode is of a three-layer structure; during preparation, a spinning solution A and a spinning solution B are prepared respectively, the patterned receiving electrode is used as a receiving device, and the micro-nano fiber patterned tissue engineering scaffold is prepared by spraying the solutions through double nozzles for electrostatic spinning. The product provided by the invention can be better matched with the mechanical behavior of a target organization; the preparation method is simple.

The invention discloses a degradable macroporous composite tissue engineering scaffold, a structural performance regulation and control method and application, and belongs to the technical field of biomedical materials. Induced phase separation is realized through design of gel molecules and a solvent, so that a macroporous structure is formed; the pore structure is adjusted by controlling the proportion of the polyvinyl alcohol aqueous solution, the glycerol and the hydrophilic degradable biomacromolecule aqueous solution; the mechanical property of the stent is regulated and controlled by adding the degradable inorganic micro-nano particles; meanwhile, a pore structure and a scaffold material with spatial heterogeneity in mechanical property are constructed through hot melt interface integration. The problems that an existing tissue engineering scaffold is insufficient in controllability of a pore structure and mechanical properties and the like are solved, and the degradable macroporous composite tissue engineering scaffold material is suitable for being used as a degradable macroporous composite tissue engineering scaffold material for promoting collaborative injury repair of soft and hard tissues such as osteochondral tissues, muscular bones and tendon bones.

The application relates to the technical field of medical materials, in particular to an antioxidant and bacteriostatic chitosan hydrogel and a preparation method thereof. The method is characterized in that lipoic acid is first grafted on a chitosan molecular chain to prepare modified chitosan; then the modified chitosan is mixed with a functional additive, a photo irradiation is performed to initiate ring-opening crosslinking of a lipoic acid disulfide five-membered ring, meanwhile, a mercapto-dithiol exchange reaction occurs between mercapto groups on the surface of the functional additive and a disulfide bond, and a three-dimensional network hydrogel is rapidly formed; the functional additive is prepared through ultraviolet light reaction of mercapto titanium oxide microspheres, a synergistic bacteriostatic agent and a modifier, and a three-dimensional bacteriostatic network layer is formed on the surface of the titanium oxide microspheres; the chitosan hydrogel prepared by the method has excellent antibacterial performance and good antioxidant performance, is suitable for the biomedical field such as wound dressings, tissue engineering scaffolds and drug sustained-release carriers, and has a wide application prospect.

The present application relates to the field of biomimetic mineralization and tissue engineering scaffold materials, and particularly relates to a kind of biomimetic mineralization silk fibroin scaffold material and its preparation method and application.The preparation method comprises the following steps:1) silk fibroin is induced self-assembly, and silk fibroin fiber matrix is obtained;2) the silk fibroin fiber obtained in step 1) is crosslinked, and crosslinked silk fibroin fiber mineralization matrix is obtained;3) the crosslinked silk fibroin fiber mineralization matrix obtained in step 2) is mineralized in mineralization solution, and biomimetic mineralization silk fibroin scaffold material is obtained.The biomimetic mineralization silk fibroin scaffold material prepared by the preparation method is applied to bone tissue repair.In the present application, the mineralization mode in silk fibroin fiber more highly simulates the mineralization form of natural bone tissue, and the similar surface chemical properties and nano-scale structure characteristics of natural bone are reproduced.

The invention discloses a preparation method and application of IBFO (at) high-entropy alloy heterojunction piezoelectric hydrogel (IBHA-SAPS), and the preparation method comprises the following steps: preparing high-entropy alloy (HEA) powder through sol-gel and calcination, compounding the HEA powder with bismuth-doped bismuth ferrite (IBFO) to construct an IBFO (at) HEA heterojunction (IBHA), and finally carrying out cross-linking molding with a sodium alginate-phenylboronic acid polymer. Under ultrasonic stimulation, the piezoelectric effect of the hydrogel can synergistically enhance the sonodynamic performance and the nano-enzyme activity, and in an acidic microenvironment, efficient antibiosis and biological membrane removal can be achieved through high-intensity ultrasound; in a neutral physiological microenvironment, low-intensity ultrasound can trigger the composite material to remove active oxygen so as to relieve oxidative stress and generate an electric signal to promote osteoblast differentiation, and the composite material has good biocompatibility, injectability and intelligent responsiveness and has wide application prospects in the fields of sonodynamic therapy, tissue engineering scaffolds, infectious bone defect repair and the like.

The embodiment of the application discloses a preparation method of humanized extracellular matrix, and belongs to the technical field of biomedical materials and tissue engineering. The method comprises the following steps: preparing human decellularized dermal matrix; preparing a concentrated solution of a condition medium rich in human active factors; constructing RGD-PLGA / BMP-2 nanocomposites; loading the concentrated solution of the condition medium into the human decellularized dermal matrix through vacuum negative pressure perfusion, and then anchoring the RGD-PLGA / BMP-2 nanocomposites, and cross-linking them twice through genipin to obtain the humanized extracellular matrix. Through the four-element strategy of decellularized matrix-active factor-nano-sustained release-two-stage cross-linking, the unity of low immunogenicity, high biological activity and spatiotemporal controllable release function is realized, and the humanized extracellular matrix can be applied to the fields of tissue engineering scaffolds, wound repair and bone defect filling.

The invention belongs to the field of biomedical engineering materials, and aims to provide a biodegradable bone repair material capable of 3D printing and a preparation method thereof. Biological 3D printing is an emerging subject of additive manufacturing and biomedicine cross fusion, and the tissue engineering scaffold obtained through biological 3D printing has good biocompatibility and excellent biological activity. The degradable bone repair material and 3D printing are combined. Degradable polylactic acid and an inorganic biological material are fused, polycaprolactone-ZnO is subjected to electrostatic spinning to enhance the supporting strength of the material, and meanwhile hydroxyapatite and trace metal elements needed by bone tissue growth are added. In clinical application, the bone repair material plays a supporting role in cell division and new tissue generation. In clinical application, the bone repair material plays a supporting role in cell division and new tissue generation. Along with gradual degradation of the repair material in a living body, the degradation product does not generate host reaction to the living body, and meanwhile, secondary trauma and medical cost caused by the fact that a patient needs to carry out a secondary operation to take out an implant material after bone repair are reduced.

The application discloses a preparation method of a multi-phase "core-shell" biomimetic tissue engineering support, and constructs a multi-phase "core-shell" in-situ biomimetic support to promote the reconstruction of a rotator cuff tendon-bone interface; the support is made of PCL / PEO biological materials and mixed with a plurality of bioactive factor microspheres, the support fiber is a "core-shell" structure, the "shell" contains SDF-1 chitosan slow-release microspheres, the "inner core" is a bone tissue layer, a fibrocartilage layer and a tendon layer, the layers are continuously printed, the spatial distribution of various factors is fine and controllable, the tendon-bone interface is reconstructed in a biomimetic manner, the sequential controllable release of SDF-1 in the outer layer and various differentiation induction factors in the inner core is realized by using the "core-shell" structure fiber, the endogenous MSCs recruitment and the ordered directional differentiation of each layer are promoted, and the application develops an electrohydrodynamic coaxial 3D printing technology on the basis of the electrohydrodynamic printing technology, and constructs a multi-phase "core-shell" in-situ biomimetic support to promote the reconstruction of the rotator cuff tendon-bone interface.

This application relates to an in-situ printing device and method for tissue engineering scaffolds. The device includes a main unit, a handheld end, and connecting cables. The main unit includes a housing, a display screen, a control system, and a high-voltage generator. The handheld end includes a body, a propulsion mechanism, and a long nozzle tube. The long nozzle tube is installed at the front end of the main unit, and the high-voltage generator is used to generate a high-voltage electric field at the front end of the long nozzle tube. The propulsion mechanism is installed inside the main unit, and a material tube for storing bio-ink is installed inside the main unit. The propulsion mechanism is connected to both the long nozzle tube and the material tube. The control system controls the propulsion mechanism to deliver the bio-ink to the long nozzle tube, and under the action of the high-voltage electric field, the bio-ink forms fibers and deposits on the area to be repaired to construct a tissue engineering scaffold. This application combines the handheld end with the main unit, and through the main unit controlling the long nozzle tube, directly forms a tissue engineering scaffold in situ inside the body, making the morphology and structure of the scaffold highly match the damaged tissue, reducing surgical risks and patient suffering.

The invention relates to photocuring bio-ink as well as a preparation method and application thereof, and belongs to the technical field of biological materials and tissue engineering. The photocuring bio-ink comprises methacrylated fish scale gelatin and a photoinitiator, the methacrylated fish scale gelatin is prepared from fish scale gelatin and methacrylic anhydride through an acylation reaction, and the substitution degree of the methacrylated fish scale gelatin is 30%-55%. The photocuring bio-ink has the advantages of fast photocuring, low immunogenicity, low fat content and excellent biocompatibility, and is especially suitable for 3D biological printing construction of fine tissue engineering scaffolds and organoids such as cartilage, skin and blood vessels.

The invention discloses a three-layer artificial soft tissue repair membrane with progressively-changed porosity in the field of biomedical tissue engineering, and the repair membrane sequentially comprises an ordered fiber layer, a spiral disordered fiber layer and an ordered fiber layer from inside to outside to form a structure with progressively-increased porosity. Each layer is made of biodegradable materials which can be selected from various natural or synthetic polymers. The diameter of the fiber is in a range from nanoscale to micron scale, and the distance between the fibers of the ordered fiber layer is adjustable and is gradually increased from inside to outside. The membrane can load various bioactive substances and drugs, and the surface of the membrane can also be modified by growth factors or nano materials and the like. The preparation method mainly adopts a near-field direct-writing 3D printing process and is realized by regulating and controlling printing parameters. The obtained repair membrane is unique in pore structure, can simulate natural tissues and promote cell ordered growth and tissue repair, and is suitable for the field of tissue engineering scaffolds.

According to the hydroxyethyl chitosan-polyethylene glycol-laponite composite hydrogel provided by the invention, polyethylene glycol, polyethylene glycol and laponite (LAPONITE) are introduced to form a mixed solution, and the prepared composite hydrogel is uniform and has good stability and mechanical properties; the compound can be clinically used as a tissue filling agent, a tissue engineering scaffold, a drug carrier, a sealing agent or an embolization agent.

The application relates to a preparation method of a tissue-adhesive hydrogel and application thereof, and belongs to the technical field of biological medicines, and solves the technical problems of poor biocompatibility, complex preparation process and difficult preservation of the tissue-adhesive hydrogel, and the solution is as follows: gelatin and casein are crosslinked by a glutamine transferase to form a hydrogel; a layer of chitosan solution is coated on an ex vivo pigskin, and then the hydrogel is covered on the ex vivo pigskin, so that the hydrogel and the ex vivo pigskin are finally adhered together. The tissue-adhesive hydrogel prepared by the application has good biocompatibility and degradability, and has the performances of transparency, water retention, anti-freezing, drug loading and local adhesion with tissues. The natural hydrogel with excellent performances has practical significance and can be applied to the fields of tissue engineering scaffolds, skin dressings, biosensors and the like.

The application discloses a composition, a preparation method and application thereof. The application provides a preparation method of the composition, the composition prepared through the preparation method can be applied to preparation of a nerve repair material, the application also provides application of the composition in reducing a hydrolysis speed of the nerve repair material and improving antioxidant capacity of the nerve repair material, the application further provides a preparation method of a tissue engineering scaffold based on the composition, the prepared tissue engineering scaffold and the aforementioned composition can be applied to a nerve repair medical device, and the application has a wide development and application prospect.

The application discloses an anisotropic three-dimensional polymer with magnetic driving form adaptability and a preparation method and application thereof. The three-dimensional polymer network is embedded with pre-prepared magnetic microfibers with high length-diameter ratio and anisotropic characteristics. Under the action of a dynamic external magnetic field such as an alternating or rotating magnetic field, the internal magnetic microfiber network can generate high-efficiency mechanical torque and anisotropic driving force, and endow the macroscopic polymer with extremely sensitive motion deformation capability and local mechanical force output. The application breaks the mechanical isotropy limitation caused by the random dispersion of nanoparticles in traditional magnetic polymers, and can prepare macroscopic gel patches, injectable microfluidic microspheres and flexible elastomers across scales, and has extremely high commercial and scientific research conversion value in the fields of micro-nano soft robot driving, biomimetic dynamic tissue engineering scaffolds, cell mechanical culture matrix and intelligent drug delivery carriers.

The invention discloses a calcium stearate modified PGA fiber for bone healing and a preparation method and application thereof, and belongs to the technical field of medical biomaterials.The preparation method comprises the steps that firstly, a silane coupling agent is used for conducting surface modification on nano-hydroxyapatite, then the nano-hydroxyapatite, PGA slices, calcium stearate powder and an optional coloring agent are mixed and dried, and the PGA fiber is obtained; finally, the composite fiber is prepared through melt spinning; wherein the calcium stearate simultaneously plays a triple synergistic role of the internal lubricant, the hydrophobic modifier and the dispersing aid, the inherent defects that the PGA melt is poor in fluidity and easy to hydrolyze are remarkably improved, the obtained fiber is excellent in processability, high in mechanical strength and controllable in-vitro degradation rate, calcium and phosphorus ions can be continuously released to promote osteogenesis, and the preparation method is simple in process, easy to operate and suitable for industrial production. The method is suitable for industrial production, and the prepared fiber can be used for bone repair materials, absorbable suture lines and tissue engineering scaffolds.

The invention discloses a three-layer artificial soft tissue composite repair membrane with progressive variable porosity and a preparation method and application of the three-layer artificial soft tissue composite repair membrane in the field of biomedical tissue engineering.The repair membrane is composed of three composite layers, and the first composite layer comprises a spiral disordered fiber layer and a first ordered fiber layer; the second composite layer comprises a spiral disordered fiber layer and a second ordered fiber layer, and the third composite layer is a middle disordered fiber layer. The middle disordered fiber layer is formed by stacking fibers which are spirally and disorderly arranged, and the ordered fiber layers on the two sides are formed by stacking fibers which are linearly arranged. And each layer of material is a biodegradable natural or synthetic polymer. The membrane can be loaded with active substances or subjected to surface modification. The preparation method adopts a thermal method and a near-field direct-writing printing process, and is realized by respectively printing each composite layer and then carrying out thermal bonding. The membrane is unique in structure, can effectively guide cell growth and promote tissue integration and regeneration, and is suitable for the field of tissue engineering scaffolds.

The present application relates to the technical field of tissue engineering scaffold, and especially relates to a tissue engineering tubular scaffold and a manufacturing method thereof, which comprises: a tubular support framework, a plurality of holes are arranged on the tube wall of the tubular support framework, the tubular support framework is etched and surface modified by low-temperature plasma; a hydrogel coating layer, which comprises an axially extending hydrogel coating inner layer and a hydrogel coating outer layer, the tubular support framework is arranged between the hydrogel coating inner layer and the hydrogel coating outer layer, and the hydrogel coating layer completely covers the tubular support framework; and the tubular support framework is used for supporting the hydrogel coating inner layer and the hydrogel coating outer layer. By being arranged in this way, the tissue engineering tubular scaffold of the present application is beneficial to the culture of tubular tissues in vitro and meets the requirements of biological performance and mechanical performance after being implanted into the body, and solves the problems of poor form retention ability, insufficient mechanical performance and poor bonding strength of different materials of the hydrogel 3D printing tissue engineering tubular scaffold.

The invention belongs to the technical field of tissue engineering scaffolds, and relates to a degradable micro-nano fiber composite tissue engineering scaffold and a preparation method thereof.The degradable micro-nano fiber composite tissue engineering scaffold is composed of evenly-distributed micron-sized skeleton fibers and evenly-distributed bioactive nano-sized filling fibers, the diameter of the micron-sized skeleton fibers is 15-25 microns, and the diameter of the bioactive nano-sized filling fibers is 10-20 microns. The diameter difference between the micron-sized skeleton fibers and the bioactive nano-sized filling fibers is more than 50 times; the bioactive nanoscale filling fibers are distributed on the surfaces of the micron-sized skeleton fibers and among different micron-sized skeleton fibers, and the micron-sized skeleton fibers and the bioactive nanoscale filling fibers penetrate through each other to form a three-dimensional graded fiber network structure; during preparation, a spinning solution A and a spinning solution B are prepared respectively, and the degradable micro-nano fiber composite tissue engineering scaffold is prepared by adopting double nozzles to spray the solutions for electrostatic spinning. The degradable micro-nano fiber composite tissue engineering scaffold disclosed by the invention has a three-dimensional interpenetrating hierarchical network structure, long-acting mechanical supporting capability and good cell compatibility.

The invention discloses a skull tissue engineering scaffold prepared by adopting a dual cold extraction freeze-drying process and a preparation method thereof, the dual cold extraction freeze-drying process is creatively proposed for the first time to realize a mild treatment process of printing, drying, crosslinking and re-drying, and while the morphology precision of the scaffold is kept, the skull tissue engineering scaffold prepared by adopting the dual cold extraction freeze-drying process is prepared. The mechanical property and the structural stability are remarkably enhanced, a brand new thought and technical support are provided for clinical conversion of 3D printing bone tissue engineering materials, and the method has a wide application prospect in the field of clinical bone repair.

The invention relates to printing ink and a preparation method and application thereof, the printing ink is composed of liquid acrylate poly (4-methyl-epsilon-caprolactone), thiol functionalized polyethylene glycol and an ultraviolet light initiator, the molar ratio of the liquid acrylate poly (4-methyl-epsilon-caprolactone) to the thiol functionalized polyethylene glycol is 1: (0.25-1.0), and the ultraviolet light initiator is a solvent. The mass of the ultraviolet light initiator is (0.95 to 1.05) percent of the mass of the liquid acrylate poly (4-methyl-epsilon-caprolactone). The invention also provides a preparation method of the main components of the ink, a preparation method of the ink and a method for preparing a 3D biodegradable elastic component by using the ink through DIW printing. The printing ink has good printability and rapid ultraviolet light curing capacity, can form an elastic component which is stable in structure, biodegradable and good in biocompatibility, and is particularly suitable for the fields of tissue engineering and the like, and the printing ink and the printing method have wide application prospects in the fields of preparation of tissue engineering scaffolds and the like.

The application provides a hydrogel prepared by cross-linking of methacrylated cross-linking monomers containing functionalized cells; the surface of the functionalized cells is coated with a first coating layer and a second coating layer; the first coating layer is an aminoated positive high molecular material or a fibronectin coating layer; the second coating layer is a 4-amine-2,2,6,6-tetramethylpiperidyl modified high molecular material coating layer; or the surface of the functionalized cells is grafted with 4-amine-2,2,6,6-tetramethylpiperidyl. The functional high molecular material grafted with 4-amine-2,2,6,6-tetramethylpiperidyl is coated on the surface of cells through electrostatic adsorption or layer-by-layer self-assembly with other high molecular materials, and the coated cells are planted into the hydrogel cross-linked by free radical chain reaction for three-dimensional culture of the cells, the polymerization of the cross-linking reaction of the hydrogel is blocked, and the microenvironment of the tissue engineering scaffold around the cells is micro-regulated.

This invention discloses a 3D printing bio-ink, a composite tissue engineering scaffold, and their 3D printing methods and applications. The 3D printing bio-ink comprises component A and component B; component A includes 500-1500 g / L β-tricalcium phosphate, 0.5-5 g / L collagen, 0-10 g / L graphene oxide, and 0.1-5 g / L chitosan and acetic acid; component B includes 100-400 g / L cellulose nanocrystals and water; the volume ratio of components A to B is (0.5-2):1. The 3D printing method for the composite tissue engineering scaffold includes the following steps: loading the 3D printing bio-ink into a 3D printer; designing and importing preset scaffold model parameters; setting the printhead specifications according to the ink properties; correcting the printing offset; determining the printing pressure and speed; setting the printhead temperature and printing platform temperature; forming the preset structure using direct-write printing; pre-drying in an oven followed by supercritical drying to obtain the composite tissue engineering scaffold. The composite tissue engineering scaffold of this invention has excellent biocompatibility and structural stability and can be used in the repair of jawbone defects and other oral tissue regeneration fields.