132 results about "Tissue engineering scaffold" patented technology

The invention relates to a protein-based phenylboronic acid ester dynamic crosslinking hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A, namely a phenylboronic acid modified protein polymer derivative, in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; and mixing the solution A and the solution B with a certain concentration for a certain time to obtain the protein-based phenylboronic acid ester dynamic crosslinking hydrogel material. The boric acid ester dynamic cross-linking bond belongs to reversible chemical cross-linking reaction, and the prepared protein-based hydrogel material has enough mechanical strength and cheap operability. The protein-based hydrogel material disclosed by the invention can provide the effects of cell adhesion, proliferation and differentiation promotion, tissue regeneration and the like, can load and slowly release beneficial components for promoting tissue repair, is an ideal tissue engineering scaffold material, and can be applied to defect regeneration and repair of soft tissues, cartilages, bones and the like.

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The embodiment of the invention discloses a protein-based biological material as well as a preparation method and application thereof. The method comprises the following steps: constructing a recombinant vector containing a coding collagen-like protein gene and a hedgehog protein gene, and performing expression and purification to obtain a recombinant protein; and reacting the recombinant protein with cholesterol in the presence of a TBS buffer solution to obtain the protein-based biological material. The mixed biological material with the functional cholesterol modified polypeptide shows programmable self-assembly on the nanoscale, has great market value potential in biomedical raw materials for treating diseases such as diabetes mellitus, tissue repair, tissue engineering scaffolds and treatment, drug delivery and the like, and has wide application prospects. And contributions are made to the development of biomedical materials in the future.

The invention relates to the technical field of biological materials, in particular to a PDRN porous microsphere as well as a preparation method and application thereof. The PDRN porous microspheres are prepared from the following raw materials: PDRN, an oil phase matrix, a curing agent and metal salt, the curing agent is at least one of polyethylene glycol acrylate and polylysine; the metal salt is at least one of calcium chloride, zinc chloride, magnesium chloride and ferric chloride. The PDRN porous microsphere has the advantages of being controllable in size, excellent in disintegration resistance and excellent in swelling resistance, is high in moist heat sterilization tolerance and strength, can be used as a filling material for medical beauty or an ideal candidate material of a tissue engineering scaffold, and has potential application value.

The invention relates to a chitosan-collagen synergistic self-assembly fiber material and a preparation method thereof, and belongs to the technical field of biological fiber materials. According to the method, the mass ratio of chitosan to collagen is controlled, co-assembly is induced through hydrogen bonds and electrostatic interaction in a low-temperature PBS solution, and then glutaraldehyde crosslinking, gradient dehydration and supercritical drying are performed to prepare the freeze-dried fiber. The obtained material completely retains the triple helix conformation of collagen, and has excellent conformation stability and enzymolysis resistance. The mechanical strength of the composite fiber is remarkably improved, and the composite fiber has excellent antibacterial property and cell affinity. The process does not need a toxic cross-linking agent, the operation is simple and convenient, and the prepared fiber is suitable for the high-end biomedical fields of tissue engineering scaffolds, intelligent antibacterial dressings, degradable nerve conduits and the like.

The invention relates to an E7 peptide grafted methacrylamide karaya harlequin tree hydrogel scaffold and a preparation method thereof, the hydrogel scaffold is a comprehensive bone tissue engineering scaffold, the diameter is 140-680 nm, and the hydrogel scaffold includes but is not limited to regeneration and repair of bone defects clinically; the methacrylamide and karaya karaya are mainly used as main base materials and grafted with E7 peptide, and the finally obtained methacrylamide karaya karaya hydrogel scaffold grafted with E7 peptide has a plurality of uniformly distributed pores and has a good intercommunication microstructure. The method is simple and clear in design, the shape and the microstructure of the scaffold can be accurately controlled, the mechanical property can be adjusted according to needs, the cost is relatively low, the method is suitable for tissue repair, and a preliminary research foundation is laid for exploring a novel tissue engineering scaffold.

The invention discloses a method for preparing PDLLA porous microspheres with high porosity and uniform particle size distribution in batches, and belongs to the technical field of polymer biomedical material preparation. According to the method, an ultrasonic-assisted double-emulsification system is combined with an inner water phase (an ammonium bicarbonate solution), an oil phase and an outer water phase (a polyvinyl alcohol solution), and a staged curing process is adopted to realize accurate regulation and control of a pore structure and a particle size. Petroleum ether is introduced as a poor solvent to optimize the precipitation uniformity, ultrasonic and mechanical stirring synergistically improve the emulsification uniformity, and staged curing ensures that the solvent evaporation rate is matched with pore formation. The porosity of the prepared PDLLA microspheres reaches 75%-87%, the SPAN value is as low as 0.512, the pore size distribution is uniform, the process expandability is high, and the method is suitable for the biomedical fields of drug carriers, tissue engineering scaffolds and the like. The technical problems of low porosity, wide particle size distribution and complex process are solved, and the method has a remarkable application prospect.

An acellular matrix woven material, a preparation method and an application thereof are provided. The acellular matrix woven material is prepared by combining 3D printing with weaving. At the same time, also provided are the acellular matrix woven material prepared by the preparation method and its application in preparing tissue engineering scaffold materials.

A method for preparing a magnesium alloy tissue engineering scaffold with a smooth inner surface by laser powder bed fusion (LPBF) is provided. The method includes: step S1: scanning a porous scaffold, and selecting the densest filling scan parameters, where the scanning includes a single-pass contour scan and a single-pass filling scan; step S2: optimizing a contour scan strategy according to the densest filling scan parameters; step S3: acquiring a corresponding melt pool dimension, and adjusting a spot compensation value based on the optimized contour scan strategy; and step S4: preparing a magnesium alloy tissue engineering scaffold based on the contour scan strategy and the adjusted spot compensation value. The method eliminates powder adhesion and sagging defects inside the complex porous structure that severely affect inner surface roughness and scaffold performance, thereby the pore connection, fatigue and corrosion resistance will be improved greatly.

The application provides a preparation method of a long-size, high-consistency and high-strength hydrogel, adopts a preparation process of directional constant-speed freezing combined with freezing cross-linking, first injects a raw material solution into a mold, then vertically immerses the mold into a cold source at a constant speed to realize directional growth of ice crystals, controls consistency and stability of the ice crystal structure, puts the directional ice crystal template into a low-temperature cross-linking system after freezing is completed, uniformly heats the system in the cross-linking process to further fix the channel structure, and finally obtains a hydrogel product with good mechanical properties and an anisotropic structure. The preparation method provided by the application can be suitable for various polymer systems, can be extended to preparation of large-size tissue engineering scaffolds and other materials, and has excellent popularization value.

The application provides a biocompatible biological aerogel material and a preparation method thereof, and the method comprises the following steps: cleaning and roughening pretreatment of a substrate; dissolving polyvinyl alcohol to prepare a precursor solution; introducing wear-resistant reinforcing materials into the precursor solution and uniformly dispersing the wear-resistant reinforcing materials; adding a crosslinking agent, glutaraldehyde, to perform a crosslinking reaction and construct a three-dimensional network structure; coating the system in the initial stage of crosslinking on the substrate, and performing low-temperature or freeze-drying and subsequent heat treatment to form a stable coating. The obtained material takes polyvinyl alcohol as a biocompatible skeleton, and has excellent porous structure, mechanical properties and wet stability through chemical crosslinking and reinforcing phase compounding. In-vitro cell experiments show that the material has no obvious toxicity to cells, and the cells adhere and spread well. The application solves the problem that the existing biological aerogel material is difficult to consider the biocompatibility, structural stability and process friendliness, and is suitable for the biomedical field such as tissue engineering scaffolds and wound dressings.

The invention provides PVDF / gelatin / CNF composite hydrogel capable of enhancing piezoelectric property based on an in-situ stretching effect and a preparation method and application thereof. According to the composite hydrogel, multi-directional constraining force is applied to a PVDF molecular chain through reaction among CN, multivalent metal ions in a cross-linking agent and PVDF; and the in-situ stretching polarization is realized by promoting the transformation from a non-piezoelectric alpha / gamma phase to a piezoelectric beta phase with an all-trans conformation. The composite hydrogel has good biocompatibility, degradability, high strength, high toughness and fatigue resistance, is mild in preparation condition and low in energy consumption, and has huge application potential in the fields of wearable stress / strain sensors, self-powered implantable medical equipment, high-performance energy collectors, bearing tissue engineering scaffolds and the like.

The present invention discloses a polydioxanone and its preparation method and application, in particular its application in medical materials (e.g., sutures). The polydioxanone has a specific molecular weight and molecular weight distribution, is highly stable, has a long shelf life, is advantageous for storage and use, and has good processing properties. The polymer fiber prepared using the polydioxanone is easily formed, has high breaking strength, and has good performance in use. The polydioxanone is suitable for preparing surgical sutures and for preparing medical dressings, anti-adhesion materials, orthopedic repair materials, tissue engineering scaffolds, and other medical materials by weaving the fibers. The polydioxanone can also be used to prepare products such as drug carriers, and has good application value. The preparation method of the polydioxanone provided by the present invention is simple to operate, has readily available raw materials, uses a small amount of catalyst, has low cost, is suitable for industrial production, and in particular can obtain polydioxanone with a more regular and narrower molecular weight distribution.

The invention belongs to the technical field of biomedical engineering materials, and provides a fibroin microsphere for nerve repair as well as a preparation method and application of the fibroin microsphere. Degummed silk is dissolved and dialyzed, and a silk protein solution is obtained; preparing a film from the silk protein solution and a film-forming agent, and dissolving in an ethanol solution to obtain silk protein microspheres; preparing a film from the silk protein microspheres and a dispersing agent; clamping between two electrode plates, and electrifying for polarization to obtain a polarized film; and dissolving the polarized film in water to obtain the fibroin piezoelectric microspheres. The fibroin piezoelectric microsphere can be used for three-dimensional culture of cells, can also be used as a tissue engineering scaffold, and is applied to induction of neural stem cell differentiation, restoration of nerve injury and treatment of neurodegenerative diseases.

The invention discloses a preparation method of a nanofiber yarn 3D printing composite scaffold, and the preparation method comprises the following steps: S1, dissolving a polymer and / or an inorganic source required by spinning in a corresponding solvent to obtain a spinning precursor solution; s2, spinning by using the spinning precursor solution in the step S1 through nanofiber spinning equipment to obtain nanofiber yarns; s3, printing by utilizing a 3D printing technology to obtain a polymer framework; and S4, weaving the nanofiber yarns in the step S2 in the polymer framework in the step S3 to obtain the nanofiber yarn 3D printing composite scaffold. The scaffold prepared by the preparation method not only has a 3D printed geometric structure, but also has high specific surface area and biological activity of nanofibers, can better simulate the microenvironment of natural tissues, can overcome the limitation of a traditional scaffold, and provides a more ideal tissue engineering scaffold.

The invention provides an enzyme-residue-free extracellular matrix microsphere as well as a preparation method and application thereof. An elutable sacrificial phase material capable of being subjected to liquid-liquid phase separation with an extracellular matrix is introduced in an extracellular matrix pelletizing process, and transglutaminase is confined in a sacrificial phase, so that the extracellular matrix microsphere is prepared. And the transglutaminase and the extracellular matrix form a spatially separated structure in the balling process. In the gel forming stage, transglutaminase catalyzes protein in an extracellular matrix on a two-phase interface to generate a cross-linking reaction, and microspheres with a three-dimensional network gel structure are formed; after cross-linking is completed, transglutaminase is removed by eluting the sacrificial phase material, and meanwhile, a porous network with a communicated structure is formed in situ in the microsphere, so that the obtained extracellular matrix microsphere still keeps good structural integrity and mechanical property while realizing no residue of enzyme with catalytic activity; the scaffold can provide stable support for cell adhesion, migration and three-dimensional culture, and is applicable to cell delivery, organoid construction, tissue engineering scaffolds and the like.

The invention discloses a macrophage hybrid scaffold as well as a construction method and application thereof, and belongs to the technical field of biological materials. The construction method comprises the following steps: extracting peripheral blood mononuclear cells, inducing the peripheral blood mononuclear cells into mature macrophages, loading the mature macrophages on a tissue engineering scaffold, and adding an immunoregulation factor to regulate the phenotype of the mature macrophages to obtain the M2 type macrophage hybrid scaffold. According to the invention, the macrophages with an immunoregulation effect are introduced into the tissue engineering scaffold, the macrophage hybrid scaffold is constructed together, and the macrophage hybrid scaffold can be used for repairing tissue defects, such as defect tissues such as skin, mucous membrane, viscera, cartilage or hard bone and the like; the macrophage hybrid scaffold can meet various requirements of providing mechanical support and bioremediation functions for tissue defect areas.

The invention provides a porous tissue engineering scaffold with a drug controlled release function as well as preparation and application of the porous tissue engineering scaffold. The preparation method of the porous tissue engineering scaffold comprises the following steps: S1) preparing double-layer nanoparticles by PLGA-PEG; and S2) preparing a composite scaffold by a low-temperature 3D printing technology. The stent prepared by the method has efficient drug controlled release capability, excellent mechanical properties, good biocompatibility and controllable degradation. The optimized porous structure promotes cell proliferation and tissue regeneration, meanwhile, low-temperature printing keeps the stability and activity of materials and drugs, and the material is widely applied to the fields of bone tissue engineering, local drug delivery and the like and shows remarkable clinical application value and market potential.

This invention provides a biomimetic periodontal complex multilayer hydrogel tissue engineering scaffold, its preparation method, and its application, relating to the technical field of tissue engineering scaffolds. The biomimetic periodontal complex multilayer hydrogel tissue engineering scaffold comprises, from the inside out, a cementum repair layer, a periodontal ligament repair layer, and an alveolar bone repair layer; the cementum repair layer and the alveolar bone repair layer include a mineralized hydrogel layer; wherein the raw materials for preparing the mineralized hydrogel layer include modified hyaluronic acid, methacrylamide type I collagen, and hydroxyapatite; the alveolar bone repair layer includes a non-mineralized hydrogel layer; wherein the raw materials for preparing the non-mineralized hydrogel layer include modified hyaluronic acid, methacrylamide type I collagen, and fibrin I. This invention precisely matches the mechanical and biological requirements of each layer of the periodontal complex through a three-layer structure, a reversibly hydrazone-regulated viscoelastic gradient, and a differentiated biochemical microenvironment of nHAp / FBN1.

The temperature-sensitive hydrogel of the present invention includes a temperature-sensitive polymer at a specific concentration. Thus, since the hydrogel may be useful for phase transition control at room temperature, allow gelation temperature and time control depending on a hydrophilicity ratio and a concentration change, and also, grow cells without toxicity, it may be applicable to various biomedical fields such as a tissue engineering scaffold.

This invention relates to the field of biomedical materials technology, and more particularly to a method for preparing a hydroxyapatite bone tissue engineering scaffold, comprising the following steps: preparing acetylated or alkylated modified cellulose nanocrystals; using the modified cellulose nanocrystals as a biodispersant to synthesize hydroxyapatite; and using the synthesized hydroxyapatite to prepare a bone tissue engineering scaffold. The preparation method of this invention is simple, requires minimal experimental conditions, and does not require expensive equipment; it also does not introduce organic surfactants, and the dispersant used is essentially harmless to the environment and organisms; the prepared HA exhibits good dispersibility; and the hydroxyapatite bone tissue engineering scaffold has good biocompatibility, which is beneficial for osteoblast proliferation and differentiation, and for the structural remodeling and construction of bone defect tissue.

The invention discloses a hydrogel material as well as a preparation method and application thereof, and belongs to the field of preparation of tissue engineering scaffolds. According to the technical scheme, the preparation method comprises the following steps: putting alginate hydrogel into a dopamine solution to prepare PDA-SA hydrogel, and putting the PDA-SA hydrogel into an expanded graphene dispersion to prepare the GR-PDA-SA hydrogel. The preparation method is applied to the aspect of conductive heart patches, and has the characteristics that the photothermal effect and the conductivity are achieved, the shape of the stent can be changed from a flat structure to an arch structure under near-infrared irradiation, and the adhesion of the stent on heart scar tissues can be well promoted.

The invention discloses a protein controllable self-assembly-based mild universal hydrogel toughening method, which is characterized in that more rigid and hydrophobic beta-folded ordered stacking structures are formed by controlling the transformation of protein macromolecular structures, and the mechanical properties of protein-based hydrogel are enhanced by cooperating with ionic physical cross-linking immobilization; through the synergistic effect of a physical network and a chemical network, the toughness, the strength, the elasticity and the anti-fatigue property of the hydrogel are remarkably improved under a completely mild condition. The method does not need toxic cross-linking agents or violent reaction conditions, has wide selectivity, is suitable for various protein raw materials, and opens up a new way for preparing the protein-based tough hydrogel, and the prepared hydrogel has wide application prospects in the fields of flexible sensors, tissue engineering scaffolds, drug sustained-release carriers, wound dressings or environmental engineering and the like.

The invention belongs to the technical field of tissue engineering scaffolds, and relates to a micro-nano fiber patterned tissue engineering scaffold and a solution electrostatic spinning preparation method thereof, and the micro-nano fiber patterned tissue engineering scaffold is formed by randomly interlacing and compounding uniformly distributed micron-scale skeleton fibers and nano-scale filling fibers on a patterned receiving electrode, the diameter of the micron-scale framework fiber is 15-25 microns, and the diameter of the nano-scale filling fiber is 200-500 nm; the nano-scale filling fibers are distributed on the surfaces of the micron-scale skeleton fibers and among different micron-scale skeleton fibers, and the micron-scale skeleton fibers and the nano-scale filling fibers penetrate through each other to form a three-dimensional graded fiber network structure; the patterned receiving electrode is of a three-layer structure; during preparation, a spinning solution A and a spinning solution B are prepared respectively, the patterned receiving electrode is used as a receiving device, and the micro-nano fiber patterned tissue engineering scaffold is prepared by spraying the solutions through double nozzles for electrostatic spinning. The product provided by the invention can be better matched with the mechanical behavior of a target organization; the preparation method is simple.

The invention discloses an electrostatic spinning nanofiber membrane for promoting jawbone defect osteogenesis and a preparation method, relates to the technical field of medical tissue engineering materials, and aims to solve the problem that a tissue engineering scaffold applied to periodontal tissue regeneration is mostly soaked in a solution, and the physical loading mode is not beneficial to long-term stable release of growth factors. In the prior art, metal ions are often used for activating osteoblasts, but the activation effect is limited, so that the metal ions are difficult to promote the regeneration of periodontal tissues because the metal ions are difficult to fully mobilize the initiative of the cells. The invention provides an electrostatic spinning nanofiber membrane for promoting jaw defect osteogenesis and a preparation method thereof. The preparation method comprises the following steps: preparing freeze-dried CGF powder; preparing a PVA / SA / nHA solution, and adding the freeze-dried CGF powder to obtain a spinning solution; and collecting the spinning solution on an aluminum foil roller to obtain the PVA / SA / CGF / nHA electrostatic spinning nanofiber membrane. Concentrated growth factors can be continuously and stably released, and osteogenic differentiation of peripheral jawbones is promoted.

The invention discloses a degradable macroporous composite tissue engineering scaffold, a structural performance regulation and control method and application, and belongs to the technical field of biomedical materials. Induced phase separation is realized through design of gel molecules and a solvent, so that a macroporous structure is formed; the pore structure is adjusted by controlling the proportion of the polyvinyl alcohol aqueous solution, the glycerol and the hydrophilic degradable biomacromolecule aqueous solution; the mechanical property of the stent is regulated and controlled by adding the degradable inorganic micro-nano particles; meanwhile, a pore structure and a scaffold material with spatial heterogeneity in mechanical property are constructed through hot melt interface integration. The problems that an existing tissue engineering scaffold is insufficient in controllability of a pore structure and mechanical properties and the like are solved, and the degradable macroporous composite tissue engineering scaffold material is suitable for being used as a degradable macroporous composite tissue engineering scaffold material for promoting collaborative injury repair of soft and hard tissues such as osteochondral tissues, muscular bones and tendon bones.

The application relates to the technical field of medical materials, in particular to an antioxidant and bacteriostatic chitosan hydrogel and a preparation method thereof. The method is characterized in that lipoic acid is first grafted on a chitosan molecular chain to prepare modified chitosan; then the modified chitosan is mixed with a functional additive, a photo irradiation is performed to initiate ring-opening crosslinking of a lipoic acid disulfide five-membered ring, meanwhile, a mercapto-dithiol exchange reaction occurs between mercapto groups on the surface of the functional additive and a disulfide bond, and a three-dimensional network hydrogel is rapidly formed; the functional additive is prepared through ultraviolet light reaction of mercapto titanium oxide microspheres, a synergistic bacteriostatic agent and a modifier, and a three-dimensional bacteriostatic network layer is formed on the surface of the titanium oxide microspheres; the chitosan hydrogel prepared by the method has excellent antibacterial performance and good antioxidant performance, is suitable for the biomedical field such as wound dressings, tissue engineering scaffolds and drug sustained-release carriers, and has a wide application prospect.

The present invention provides graphene oxide-based liquid crystals, binary liquid crystal systems, 3D sponge-like scaffold materials, liquid crystal gels, and biological tissue engineering scaffolds, relating to the technical field of repair medical materials. The present invention grafts cholesterol-polyethylene glycol-amino groups onto graphene oxide to prepare a novel liquid crystal composite material, a graphene oxide-based liquid crystal composite. Its aqueous solution exhibits birefringence, and the liquid crystal gel has an anisotropic structure. It exhibits good osteogenic activity, printability, biocompatibility, degradability, mechanical properties highly similar to bone tissue, and biomimetic properties. It is a novel multifunctional integrated nanostructure platform. The graphene oxide-based liquid crystal composite can be compounded with biomedical materials, photoinitiators, growth factors, and stem cell chemotactic factors to obtain tissue engineering scaffolds through 3D printing-photocrosslinking, showing promising application prospects in bone repair, cartilage repair, vascular repair, tendon repair, or myocardial repair.

The present invention discloses a bone tissue engineering scaffold, including a hollow grid-shaped scaffold and a porous zinc-based metal ball filled therein, the porous zinc-based metal ball can be a solid ball with holes and grooves on the surface or a fluffy ball rolled by metal wire; an internal support column can also be arranged in the scaffold to provide more mechanical properties; β-TCP particles can be filled to enrich bone marrow mesenchymal stem cells. The present invention combines a grid scaffold prepared by titanium or zinc-based metal and its internal support column to prepare a bone tissue engineering scaffold with excellent mechanical properties, wherein the porous zinc-based metal ball can promote osteogenic differentiation and inhibit osteoclast differentiation, while providing support, promote the regeneration and ingrowth of bone tissue, and then gradually be replaced by bone tissue during the degradation process, and β-TCP particles can enrich mesenchymal stem cells and promote bone repair. The present invention can be applied to large bone defects in load-bearing parts, promote bone tissue regeneration on the basis of mechanical support, and achieve the therapeutic effect of repairing large bone defects.

The invention discloses a regulatable and degradable succinamide ester bond hydrogel as well as a preparation method and application thereof, and belongs to the field of biomedical materials. The hydrogel is formed by covalent cross-linking of a succinate bonded polymer and a high-molecular polymer containing a plurality of amino groups through a succinamide ester bond, the hydrogel utilizes a special cyclization degradation mechanism of a succinamide ester bond, steric hindrance can be adjusted through side group modification, and the amide nitrogen deprotonation rate can be adjusted through modification of proton donor and proton acceptor groups, so that accurate control of the degradation time of the hydrogel is realized, and the degradation time can be from several hours to several months; the hydrogel can be widely applied to the biomedical fields of tissue engineering scaffolds, wound dressings / drug controlled release systems and the like.